Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0920980 | PNX91063.1 | 91.803 | 122 | 10 | 0 | 1 | 122 | 1 | 122 | 9.72e-74 | 233 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0193560 | Msa0920980 | 0.807152 | 5.811898e-50 | -8.615850e-47 |
| Msa0314050 | Msa0920980 | 0.816081 | 6.644352e-52 | -8.615850e-47 |
| Msa0920980 | Msa0920990 | 0.910371 | 2.019746e-82 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0920980 | MtrunA17_Chr6g0474221 | 44.340 | 106 | 59 | 0 | 15 | 120 | 15 | 120 | 2.87e-29 | 108 |
| Msa0920980 | MtrunA17_Chr3g0083121 | 45.283 | 106 | 58 | 0 | 15 | 120 | 14 | 119 | 3.78e-29 | 109 |
| Msa0920980 | MtrunA17_Chr8g0343501 | 43.137 | 102 | 58 | 0 | 12 | 113 | 72 | 173 | 2.03e-28 | 102 |
| Msa0920980 | MtrunA17_Chr3g0145811 | 43.119 | 109 | 62 | 0 | 12 | 120 | 14 | 122 | 5.20e-28 | 103 |
| Msa0920980 | MtrunA17_Chr2g0302521 | 39.655 | 116 | 70 | 0 | 5 | 120 | 18 | 133 | 8.52e-27 | 102 |
| Msa0920980 | MtrunA17_Chr7g0264751 | 42.453 | 106 | 61 | 0 | 15 | 120 | 33 | 138 | 8.53e-26 | 100 |
| Msa0920980 | MtrunA17_Chr1g0172701 | 30.631 | 111 | 75 | 1 | 10 | 120 | 36 | 144 | 4.01e-17 | 75.5 |
| Msa0920980 | MtrunA17_Chr1g0179951 | 34.000 | 100 | 65 | 1 | 17 | 116 | 27 | 125 | 2.52e-15 | 70.5 |
| Msa0920980 | MtrunA17_Chr1g0163881 | 34.667 | 75 | 49 | 0 | 46 | 120 | 1 | 75 | 9.85e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0920980 | AT1G21280.1 | 31.481 | 108 | 72 | 2 | 11 | 116 | 12 | 119 | 1.48e-14 | 67.8 |
Find 27 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCTTGTTCTTAGAAATTA+AGG | 0.221569 | 6_2:-56916882 | None:intergenic |
| GGCTTTATCGATCCATAAAA+TGG | 0.240429 | 6_2:-56917029 | None:intergenic |
| TCATTAGGATGAAGGTAATA+AGG | 0.304075 | 6_2:-56916782 | None:intergenic |
| TCGATAAAGCCTCCTCTGTT+TGG | 0.349971 | 6_2:+56917041 | Msa0920980:CDS |
| AGAAATTAAGGCTACCTTCA+TGG | 0.400827 | 6_2:-56916870 | None:intergenic |
| TGAAGGTAATAAGGATTTGA+AGG | 0.442728 | 6_2:-56916773 | None:intergenic |
| AGAATTACTCCTCTTGGTCT+CGG | 0.457989 | 6_2:+56916846 | Msa0920980:CDS |
| CTCTTGGTCTCGGGCCATGA+AGG | 0.471034 | 6_2:+56916856 | Msa0920980:CDS |
| ACAGCAAGAATTACTCCTCT+TGG | 0.478145 | 6_2:+56916840 | Msa0920980:CDS |
| GAATTACTCCTCTTGGTCTC+GGG | 0.493728 | 6_2:+56916847 | Msa0920980:CDS |
| GTAATAAGGATTTGAAGGGT+TGG | 0.496861 | 6_2:-56916768 | None:intergenic |
| CGAATGTCTTGCACGAGCAA+TGG | 0.505923 | 6_2:+56916942 | Msa0920980:CDS |
| GGTGTAACAAGAATTAAAGA+GGG | 0.508292 | 6_2:-56916809 | None:intergenic |
| AGGTGTAACAAGAATTAAAG+AGG | 0.528340 | 6_2:-56916810 | None:intergenic |
| TGCAAGACATTCGTAGATGA+AGG | 0.540608 | 6_2:-56916932 | None:intergenic |
| ATAAGGATTTGAAGGGTTGG+TGG | 0.562633 | 6_2:-56916765 | None:intergenic |
| TAATTCTTGCTGTCAAGTAA+AGG | 0.567589 | 6_2:-56916830 | None:intergenic |
| GCAACAACATGGTTCTCTCA+TGG | 0.569862 | 6_2:+56916972 | Msa0920980:CDS |
| GAAGGTAATAAGGATTTGAA+GGG | 0.572675 | 6_2:-56916772 | None:intergenic |
| AAGATTTGTCCAAACAGAGG+AGG | 0.605431 | 6_2:-56917050 | None:intergenic |
| TGCAAAGATGTCGACAGCCA+TGG | 0.605635 | 6_2:-56916735 | None:intergenic |
| AGAGAACCATGTTGTTGCAG+CGG | 0.624350 | 6_2:-56916967 | None:intergenic |
| AACAAGCTTCGATTTGTCGA+CGG | 0.630294 | 6_2:+56916896 | Msa0920980:CDS |
| TTCAAGATTTGTCCAAACAG+AGG | 0.631317 | 6_2:-56917053 | None:intergenic |
| GTGTAACAAGAATTAAAGAG+GGG | 0.636196 | 6_2:-56916808 | None:intergenic |
| CTTCATGGCCCGAGACCAAG+AGG | 0.643481 | 6_2:-56916855 | None:intergenic |
| ATGGATCCGCTGCAACAACA+TGG | 0.644037 | 6_2:+56916961 | Msa0920980:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTATTTCTAAATCCATTTTA+TGG | + | chr6_2:56917017-56917036 | Msa0920980:CDS | 20.0% |
| !! | AAGCTTGTTCTTAGAAATTA+AGG | - | chr6_2:56916885-56916904 | None:intergenic | 25.0% |
| GAAGGTAATAAGGATTTGAA+GGG | - | chr6_2:56916775-56916794 | None:intergenic | 30.0% | |
| TGAAGGTAATAAGGATTTGA+AGG | - | chr6_2:56916776-56916795 | None:intergenic | 30.0% | |
| TCATTAGGATGAAGGTAATA+AGG | - | chr6_2:56916785-56916804 | None:intergenic | 30.0% | |
| ! | ATTAAAGAGGGGTTTTCATT+AGG | - | chr6_2:56916800-56916819 | None:intergenic | 30.0% |
| GTGTAACAAGAATTAAAGAG+GGG | - | chr6_2:56916811-56916830 | None:intergenic | 30.0% | |
| GGTGTAACAAGAATTAAAGA+GGG | - | chr6_2:56916812-56916831 | None:intergenic | 30.0% | |
| AGGTGTAACAAGAATTAAAG+AGG | - | chr6_2:56916813-56916832 | None:intergenic | 30.0% | |
| ! | TAATTCTTGCTGTCAAGTAA+AGG | - | chr6_2:56916833-56916852 | None:intergenic | 30.0% |
| !! | TTGAACTTCGTTTTTCACAA+GGG | + | chr6_2:56917071-56917090 | Msa0920980:CDS | 30.0% |
| GTAATAAGGATTTGAAGGGT+TGG | - | chr6_2:56916771-56916790 | None:intergenic | 35.0% | |
| AGAAATTAAGGCTACCTTCA+TGG | - | chr6_2:56916873-56916892 | None:intergenic | 35.0% | |
| ! | GGCTTTATCGATCCATAAAA+TGG | - | chr6_2:56917032-56917051 | None:intergenic | 35.0% |
| TTCAAGATTTGTCCAAACAG+AGG | - | chr6_2:56917056-56917075 | None:intergenic | 35.0% | |
| !! | CTTGAACTTCGTTTTTCACA+AGG | + | chr6_2:56917070-56917089 | Msa0920980:CDS | 35.0% |
| !! | TGAACTTCGTTTTTCACAAG+GGG | + | chr6_2:56917072-56917091 | Msa0920980:CDS | 35.0% |
| ATAAGGATTTGAAGGGTTGG+TGG | - | chr6_2:56916768-56916787 | None:intergenic | 40.0% | |
| ! | AGGGGTTTTCATTAGGATGA+AGG | - | chr6_2:56916793-56916812 | None:intergenic | 40.0% |
| ACAGCAAGAATTACTCCTCT+TGG | + | chr6_2:56916840-56916859 | Msa0920980:CDS | 40.0% | |
| AGAATTACTCCTCTTGGTCT+CGG | + | chr6_2:56916846-56916865 | Msa0920980:CDS | 40.0% | |
| ! | AACAAGCTTCGATTTGTCGA+CGG | + | chr6_2:56916896-56916915 | Msa0920980:CDS | 40.0% |
| TGCAAGACATTCGTAGATGA+AGG | - | chr6_2:56916935-56916954 | None:intergenic | 40.0% | |
| AAGATTTGTCCAAACAGAGG+AGG | - | chr6_2:56917053-56917072 | None:intergenic | 40.0% | |
| GAATTACTCCTCTTGGTCTC+GGG | + | chr6_2:56916847-56916866 | Msa0920980:CDS | 45.0% | |
| AGAGAACCATGTTGTTGCAG+CGG | - | chr6_2:56916970-56916989 | None:intergenic | 45.0% | |
| GCAACAACATGGTTCTCTCA+TGG | + | chr6_2:56916972-56916991 | Msa0920980:CDS | 45.0% | |
| TCGATAAAGCCTCCTCTGTT+TGG | + | chr6_2:56917041-56917060 | Msa0920980:CDS | 45.0% | |
| CGAATGTCTTGCACGAGCAA+TGG | + | chr6_2:56916942-56916961 | Msa0920980:CDS | 50.0% | |
| ATGGATCCGCTGCAACAACA+TGG | + | chr6_2:56916961-56916980 | Msa0920980:CDS | 50.0% | |
| CTTCATGGCCCGAGACCAAG+AGG | - | chr6_2:56916858-56916877 | None:intergenic | 60.0% | |
| CTCTTGGTCTCGGGCCATGA+AGG | + | chr6_2:56916856-56916875 | Msa0920980:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 56916737 | 56917105 | 56916737 | ID=Msa0920980;Name=Msa0920980 |
| chr6_2 | mRNA | 56916737 | 56917105 | 56916737 | ID=Msa0920980-mRNA-1;Parent=Msa0920980;Name=Msa0920980-mRNA-1;_AED=0.69;_eAED=0.69;_QI=0|-1|0|1|-1|1|1|0|122 |
| chr6_2 | exon | 56916737 | 56917105 | 56916737 | ID=Msa0920980-mRNA-1:exon:8543;Parent=Msa0920980-mRNA-1 |
| chr6_2 | CDS | 56916737 | 56917105 | 56916737 | ID=Msa0920980-mRNA-1:cds;Parent=Msa0920980-mRNA-1 |
| Gene Sequence |
| Protein sequence |
