AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0921230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0921230 MtrunA17_Chr6g0486531 77.686 121 25 1 31 151 648 766 2.80e-56 189
Msa0921230 MtrunA17_Chr6g0486531 60.331 121 46 1 31 151 889 1007 7.86e-42 147
Msa0921230 MtrunA17_Chr6g0477831 86.364 88 12 0 29 116 3 90 5.52e-51 157
Msa0921230 MtrunA17_Chr6g0460441 57.522 113 25 2 31 143 52 141 4.35e-33 114
Msa0921230 MtrunA17_Chr2g0299581 50.862 116 42 2 37 151 43 144 1.61e-30 108
Msa0921230 MtrunA17_Chr6g0486301 74.390 82 11 1 54 135 1033 1104 1.44e-29 112
Msa0921230 MtrunA17_Chr6g0484381 42.975 121 38 2 31 151 44 133 1.31e-21 85.5
Msa0921230 MtrunA17_Chr6g0486461 50.820 61 30 0 91 151 8 68 2.02e-13 62.4
Msa0921230 MtrunA17_Chr6g0486491 41.463 82 34 1 37 118 27 94 3.47e-12 58.9
Msa0921230 MtrunA17_Chr6g0486281 59.574 47 19 0 89 135 1 47 3.83e-12 57.8
Msa0921230 MtrunA17_Chr6g0486321 36.036 111 41 2 41 151 87 167 7.89e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0921230 AT4G29060.1 66.116 121 39 1 31 151 558 676 2.47e-46 161
Msa0921230 AT4G29060.1 58.678 121 48 1 31 151 796 914 6.05e-38 137
Msa0921230 AT4G29060.2 57.025 121 44 2 31 151 558 670 3.84e-35 129

Find 43 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACTTTGGTTCAGTCTTCT+TGG 0.190585 6_2:-58187117 None:intergenic
GAGAAAATACGAAAGTTAAA+AGG 0.260205 6_2:+58186961 Msa0921230:three_prime_UTR
AAATTCCTCAGGAGTGTCTT+TGG 0.320407 6_2:-58186759 None:intergenic
GTTCAATCTCGGAGAGGGTT+TGG 0.344856 6_2:+58186992 Msa0921230:three_prime_UTR
AAGCAGACAATTGCAACTAT+TGG 0.347712 6_2:+58186939 Msa0921230:three_prime_UTR
GGTAACGACTCCAGTGAAAA+AGG 0.356740 6_2:+58187072 Msa0921230:three_prime_UTR
CTCTCTGAAAATTTCACCTT+GGG 0.410413 6_2:-58186678 None:intergenic
TGGTAACAACATTCTCTACT+TGG 0.432028 6_2:-58186739 None:intergenic
GGACTGTACAAGAGCTCCTT+AGG 0.433901 6_2:+58186551 Msa0921230:CDS
CAACTTCAGCAGCAAAATCC+TGG 0.440132 6_2:-58187025 None:intergenic
CAACACTTGTGAAGCAACTT+CGG 0.440747 6_2:+58186505 Msa0921230:CDS
TCTTTCTTCACAAATTCCTC+AGG 0.442126 6_2:-58186770 None:intergenic
GGTAACAACATTCTCTACTT+GGG 0.462914 6_2:-58186738 None:intergenic
AGAAATAGGTGCAGGAATGA+TGG 0.468499 6_2:+58188129 Msa0921230:intron
GGATTTAGCGTTGCTTGAGC+AGG 0.471273 6_2:+58186886 Msa0921230:CDS
CGAAGCAGAAGCTGGAAAGA+TGG 0.484065 6_2:+58186530 Msa0921230:CDS
CGAAGTTGCTTCACAAGTGT+TGG 0.484421 6_2:-58186504 None:intergenic
CTTCGGGACGAAGCAGAAGC+TGG 0.490090 6_2:+58186522 Msa0921230:CDS
CAGGTTGAAGACTTCCATGT+TGG 0.494557 6_2:+58184074 None:intergenic
GTTCTTACATTCATGACAGC+AGG 0.504575 6_2:+58186612 Msa0921230:CDS
AACACTTGTGAAGCAACTTC+GGG 0.511081 6_2:+58186506 Msa0921230:CDS
GGGACATGCCGCCACTTGCA+TGG 0.511736 6_2:-58186717 None:intergenic
AACCGAAACAAAATAGATCA+AGG 0.513980 6_2:+58186831 Msa0921230:CDS
AACGGAGATAGAAATGCAAA+AGG 0.524297 6_2:+58186793 Msa0921230:CDS
AATAGATCAAGGATTGTTGA+AGG 0.524316 6_2:+58186842 Msa0921230:CDS
CTTAAAGAGATCAGCCAACA+TGG 0.525967 6_2:-58184088 None:intergenic
GGAATTTGTGAAGAAAGAAA+CGG 0.529911 6_2:+58186775 Msa0921230:CDS
AACAGAAGGTGATCTTGAAA+AGG 0.530288 6_2:+58188174 Msa0921230:three_prime_UTR
TACATTCATGACAGCAGGAT+CGG 0.532555 6_2:+58186617 Msa0921230:CDS
GGGTTTGGAAAAGAAAAGCC+AGG 0.535470 6_2:+58187007 Msa0921230:three_prime_UTR
GTGAGGTTCAATCTCGGAGA+GGG 0.537237 6_2:+58186987 Msa0921230:three_prime_UTR
ACTCTCTGAAAATTTCACCT+TGG 0.543539 6_2:-58186679 None:intergenic
GTAACAACATTCTCTACTTG+GGG 0.553869 6_2:-58186737 None:intergenic
CGAAAGTTAAAAGGTTTGTG+AGG 0.557279 6_2:+58186970 Msa0921230:three_prime_UTR
AAAGCTCTTGCTGAAACAGA+AGG 0.590129 6_2:+58188160 Msa0921230:three_prime_UTR
AGGTTTGTGAGGTTCAATCT+CGG 0.617664 6_2:+58186981 Msa0921230:three_prime_UTR
TGATATAGCCATGCAAGTGG+CGG 0.622540 6_2:+58186709 Msa0921230:CDS
GAGACAGATTTCGTCTCCCA+AGG 0.623656 6_2:+58186662 Msa0921230:CDS
GTGCAGACAAGAAAACAGCT+AGG 0.648547 6_2:+58186590 Msa0921230:CDS
TGTGAGGTTCAATCTCGGAG+AGG 0.653160 6_2:+58186986 Msa0921230:three_prime_UTR
GGATTGTTGAAGGACAGATA+AGG 0.664515 6_2:+58186852 Msa0921230:CDS
TGCTGATATAGCCATGCAAG+TGG 0.679395 6_2:+58186706 Msa0921230:CDS
TGTTACCAAAGACACTCCTG+AGG 0.752881 6_2:+58186754 Msa0921230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAACATATATATAAGTA+TGG - chr6_2:58187795-58187814 None:intergenic 10.0%
!!! GTTATTTAATTACGAAATTT+TGG + chr6_2:58187177-58187196 Msa0921230:intron 15.0%
!! GAAACTTTAAATTAAGATAA+AGG - chr6_2:58187254-58187273 None:intergenic 15.0%
!! AAGAAAAATTGAGAAAAAAA+TGG + chr6_2:58188014-58188033 Msa0921230:intron 15.0%
!!! TTTACATTCTTTCATTTTCT+TGG + chr6_2:58184293-58184312 Msa0921230:intron 20.0%
!! TTTATGCAAATATTTCCTAT+TGG - chr6_2:58184526-58184545 None:intergenic 20.0%
!! TAAAAACTTTGGATAAACTT+TGG - chr6_2:58185183-58185202 None:intergenic 20.0%
!! TAAACTTTGGATAAAAACTT+TGG - chr6_2:58185194-58185213 None:intergenic 20.0%
!! AGTTGAAATGAAAGTTATAT+CGG - chr6_2:58185535-58185554 None:intergenic 20.0%
!!! AAGATTTTCTACAAATTAAC+TGG - chr6_2:58185668-58185687 None:intergenic 20.0%
!!! CTTCTTTTTTTTTTTTGCTA+AGG + chr6_2:58185821-58185840 Msa0921230:intron 20.0%
!!! ACAAAACAAAAGTTAATTTG+AGG - chr6_2:58186042-58186061 None:intergenic 20.0%
!!! TAAGGTAAAAACAACTTTTA+TGG - chr6_2:58186275-58186294 None:intergenic 20.0%
!! AACAATTAGAAATCTTATGT+AGG - chr6_2:58186441-58186460 None:intergenic 20.0%
!!! AAATTTTGGTAATTAGTTTC+TGG + chr6_2:58187191-58187210 Msa0921230:intron 20.0%
!!! TCTTAATTTAAAGTTTCAAC+AGG + chr6_2:58187257-58187276 Msa0921230:intron 20.0%
!!! TTAAAGGTACATTATATCTT+CGG + chr6_2:58187401-58187420 Msa0921230:intron 20.0%
!!! ATACTATGTCTTAATTTTGA+TGG - chr6_2:58187479-58187498 None:intergenic 20.0%
!! AACTATGGAAATGTTATAAT+GGG - chr6_2:58187536-58187555 None:intergenic 20.0%
!! TAACTATGGAAATGTTATAA+TGG - chr6_2:58187537-58187556 None:intergenic 20.0%
!!! TCACTTTTGAATTATACTAA+AGG + chr6_2:58187704-58187723 Msa0921230:intron 20.0%
!! AACATATATATAAGTATGGA+GGG - chr6_2:58187791-58187810 None:intergenic 20.0%
!! TAACATATATATAAGTATGG+AGG - chr6_2:58187792-58187811 None:intergenic 20.0%
!! AATATTATCTGAATTGTTCT+TGG + chr6_2:58187812-58187831 Msa0921230:intron 20.0%
! AAATGAAACAACATTTCTCT+AGG - chr6_2:58184172-58184191 None:intergenic 25.0%
!! TTTTACACTCCATAACTAAT+AGG - chr6_2:58184466-58184485 None:intergenic 25.0%
!!! TTTTTTATTATGCCACCAAT+AGG + chr6_2:58184508-58184527 Msa0921230:intron 25.0%
!! TTTTACACTCCATAACTAAT+AGG - chr6_2:58184466-58184485 None:intergenic 25.0%
! TTTATGCAAATATTTCCTAC+TGG - chr6_2:58184837-58184856 None:intergenic 25.0%
! TTTATGCAAATATTTCCTAC+TGG - chr6_2:58184837-58184856 None:intergenic 25.0%
! TTTATGCAAATATTTCCTAC+TGG - chr6_2:58184837-58184856 None:intergenic 25.0%
! AATGGTTATATACTTCAAAG+TGG - chr6_2:58185270-58185289 None:intergenic 25.0%
! ATTAAACTGGTAAGATTCAT+TGG - chr6_2:58185423-58185442 None:intergenic 25.0%
!! TTGATGTTGTTTGATTAAAC+TGG - chr6_2:58185436-58185455 None:intergenic 25.0%
! AACTTTCATTTCAACTTAAG+TGG + chr6_2:58185539-58185558 Msa0921230:intron 25.0%
! TCAACTTAAGTGGTAAAATT+TGG + chr6_2:58185549-58185568 Msa0921230:intron 25.0%
!!! TTTTTTTTTTGCTAAGGTTC+AGG + chr6_2:58185827-58185846 Msa0921230:intron 25.0%
!! TTGATGGTTATTTACTTTCA+TGG + chr6_2:58185860-58185879 Msa0921230:intron 25.0%
! TAATGCAACAAAAATCTTCT+TGG - chr6_2:58186149-58186168 None:intergenic 25.0%
! AAAAAAAACATTAGCCATGA+GGG - chr6_2:58186185-58186204 None:intergenic 25.0%
! AAAAAAAAACATTAGCCATG+AGG - chr6_2:58186186-58186205 None:intergenic 25.0%
! TTATGTAGGAATTCAAAGAT+TGG - chr6_2:58186427-58186446 None:intergenic 25.0%
!!! ATCCTTGATCTATTTTGTTT+CGG - chr6_2:58186836-58186855 None:intergenic 25.0%
! GAGAAAATACGAAAGTTAAA+AGG + chr6_2:58186961-58186980 Msa0921230:three_prime_UTR 25.0%
!!! GGTTGTTTTGTTTAAAATGT+AGG + chr6_2:58187329-58187348 Msa0921230:intron 25.0%
!! AAAATGATCAACTAGACTTT+TGG - chr6_2:58187382-58187401 None:intergenic 25.0%
!!! GTCTAGTTGATCATTTTTAA+AGG + chr6_2:58187385-58187404 Msa0921230:intron 25.0%
AGTACAATTCAAGTGATGAT+TGG + chr6_2:58184142-58184161 Msa0921230:intron 30.0%
TTTGTGTCTAATACTCTTCA+TGG - chr6_2:58184386-58184405 None:intergenic 30.0%
ATGCAAATATTTCCTATTGG+TGG - chr6_2:58184523-58184542 None:intergenic 30.0%
TGTGAATGAATGATAACTTC+GGG - chr6_2:58184631-58184650 None:intergenic 30.0%
CTGTGAATGAATGATAACTT+CGG - chr6_2:58184632-58184651 None:intergenic 30.0%
TTTGTGTCTAATACTCTTCA+CGG - chr6_2:58184386-58184405 None:intergenic 30.0%
!!! TTTTTTATTATGCCACCAGT+AGG + chr6_2:58184819-58184838 Msa0921230:intron 30.0%
!!! TTTTTTATTATGCCACCAGT+AGG + chr6_2:58184819-58184838 Msa0921230:intron 30.0%
!!! TTTTTTATTATGCCACCAGT+AGG + chr6_2:58184819-58184838 Msa0921230:intron 30.0%
TGTGAATGAATGATAACTTC+GGG - chr6_2:58184631-58184650 None:intergenic 30.0%
GTGTGAATGAATGATAACTT+CGG - chr6_2:58185219-58185238 None:intergenic 30.0%
AAAACTTCAAGAAGCTAGTA+GGG - chr6_2:58185335-58185354 None:intergenic 30.0%
ATCTTGTATGTTCTGTTGTA+TGG + chr6_2:58185783-58185802 Msa0921230:intron 30.0%
GTAGCAACACTTAAATCAAT+AGG + chr6_2:58185919-58185938 Msa0921230:intron 30.0%
AAAAGCCTGAGATATAAAGA+TGG - chr6_2:58185987-58186006 None:intergenic 30.0%
! CTTTTAGTGATTGTTGGATT+TGG + chr6_2:58186002-58186021 Msa0921230:intron 30.0%
AAAAAAACATTAGCCATGAG+GGG - chr6_2:58186184-58186203 None:intergenic 30.0%
CTGAACAACTTATGGAAATA+AGG - chr6_2:58186293-58186312 None:intergenic 30.0%
! AAGCTTTTCTGAACAACTTA+TGG - chr6_2:58186301-58186320 None:intergenic 30.0%
GGAATTTGTGAAGAAAGAAA+CGG + chr6_2:58186775-58186794 Msa0921230:CDS 30.0%
AACCGAAACAAAATAGATCA+AGG + chr6_2:58186831-58186850 Msa0921230:CDS 30.0%
AATAGATCAAGGATTGTTGA+AGG + chr6_2:58186842-58186861 Msa0921230:CDS 30.0%
!!! GTTTTTTTTTGCAGTTTGTG+CGG - chr6_2:58187051-58187070 None:intergenic 30.0%
CACAAACTGCAAAAAAAAAC+CGG + chr6_2:58187051-58187070 Msa0921230:three_prime_UTR 30.0%
ATTCGTAGAACTACTTACTT+TGG - chr6_2:58187134-58187153 None:intergenic 30.0%
CTCACTAATAACTAACAAGT+AGG - chr6_2:58187158-58187177 None:intergenic 30.0%
CTGGAAAACTACATTCTAAA+AGG + chr6_2:58187210-58187229 Msa0921230:intron 30.0%
TAAAAGGAAAAACTACAGCA+GGG + chr6_2:58187226-58187245 Msa0921230:intron 30.0%
TATATATAAGTATGGAGGGA+AGG - chr6_2:58187787-58187806 None:intergenic 30.0%
! GTCATACTTAATCACTGATT+AGG - chr6_2:58187854-58187873 None:intergenic 30.0%
AAATTGTAATGTTGTGTTGC+CGG + chr6_2:58188057-58188076 Msa0921230:intron 30.0%
TTGAATTGTACTCCCAATGA+GGG - chr6_2:58184134-58184153 None:intergenic 35.0%
!! TCAATGTTGCTTCTGACTTT+TGG + chr6_2:58184199-58184218 Msa0921230:intron 35.0%
CTTGGCAAATAAGATTGAGT+TGG + chr6_2:58184311-58184330 Msa0921230:intron 35.0%
!! AAACTGGGGTTCTAAAAATG+TGG + chr6_2:58184360-58184379 Msa0921230:intron 35.0%
! AGAGTATTAGACACAAACCA+TGG + chr6_2:58184389-58184408 Msa0921230:intron 35.0%
TCCATGTCATATTCTCATAC+TGG + chr6_2:58184433-58184452 Msa0921230:intron 35.0%
TCCAGTATGAGAATATGACA+TGG - chr6_2:58184437-58184456 None:intergenic 35.0%
GGATTTGCACCTATTAGTTA+TGG + chr6_2:58184454-58184473 Msa0921230:intron 35.0%
GATAACTTCGGGATAAACTT+TGG - chr6_2:58184620-58184639 None:intergenic 35.0%
TCATTCACAGATTAAGATGC+AGG + chr6_2:58184642-58184661 Msa0921230:intron 35.0%
CATTCACAGATTAAGATGCA+GGG + chr6_2:58184643-58184662 Msa0921230:intron 35.0%
!! AAACTGGGGTTCTAAAAATG+TGG + chr6_2:58184360-58184379 Msa0921230:intron 35.0%
! AGAGTATTAGACACAAACCA+TGG + chr6_2:58184389-58184408 Msa0921230:intron 35.0%
TCCAGTATGAGAATATGACA+CGG - chr6_2:58184437-58184456 None:intergenic 35.0%
GGATTTGCACCTATTAGTTA+TGG + chr6_2:58184454-58184473 Msa0921230:intron 35.0%
ATGCAAATATTTCCTACTGG+TGG - chr6_2:58184834-58184853 None:intergenic 35.0%
ATGCAAATATTTCCTACTGG+TGG - chr6_2:58184834-58184853 None:intergenic 35.0%
ATGCAAATATTTCCTACTGG+TGG - chr6_2:58184834-58184853 None:intergenic 35.0%
GATAACTTCGGGATAAACTT+TGG - chr6_2:58184620-58184639 None:intergenic 35.0%
CTGAAAGTAGTAGTAGACAA+TGG - chr6_2:58185288-58185307 None:intergenic 35.0%
CACTAAAAATAGTCCCACAT+CGG - chr6_2:58185311-58185330 None:intergenic 35.0%
AAACTTCAAGAAGCTAGTAG+GGG - chr6_2:58185334-58185353 None:intergenic 35.0%
GAAAACTTCAAGAAGCTAGT+AGG - chr6_2:58185336-58185355 None:intergenic 35.0%
GCAATGTTGTATGCTACAAA+GGG - chr6_2:58185496-58185515 None:intergenic 35.0%
CTTTGTAGCATACAACATTG+CGG + chr6_2:58185495-58185514 Msa0921230:intron 35.0%
TTTGTAGCATACAACATTGC+GGG + chr6_2:58185496-58185515 Msa0921230:intron 35.0%
! TTTTCCGCATAATTAGCGAT+TGG - chr6_2:58185584-58185603 None:intergenic 35.0%
ATGCGGAAAAGACATAATGT+AGG + chr6_2:58185594-58185613 Msa0921230:intron 35.0%
TGCGGAAAAGACATAATGTA+GGG + chr6_2:58185595-58185614 Msa0921230:intron 35.0%
AATGTAGGGGGTTAATGTTA+AGG + chr6_2:58185609-58185628 Msa0921230:intron 35.0%
!!! TTTTTTGCTAAGGTTCAGGT+AGG + chr6_2:58185831-58185850 Msa0921230:intron 35.0%
!! TGTTGCTACTTTCTGAATAG+AGG - chr6_2:58185908-58185927 None:intergenic 35.0%
CCTTTACTAAAGATGAAGCA+AGG - chr6_2:58185953-58185972 None:intergenic 35.0%
CCTTGCTTCATCTTTAGTAA+AGG + chr6_2:58185950-58185969 Msa0921230:intron 35.0%
TCAAGTGAAGCTATGAAACA+TGG + chr6_2:58186114-58186133 Msa0921230:intron 35.0%
TAAGCATTTGTGAGACTGTT+TGG + chr6_2:58186219-58186238 Msa0921230:intron 35.0%
! TTAAGTTGCTTATCCAAACG+AGG + chr6_2:58186379-58186398 Msa0921230:intron 35.0%
GAATTCAAAGATTGGTGAAC+AGG - chr6_2:58186419-58186438 None:intergenic 35.0%
! CTCTCTGAAAATTTCACCTT+GGG - chr6_2:58186681-58186700 None:intergenic 35.0%
! ACTCTCTGAAAATTTCACCT+TGG - chr6_2:58186682-58186701 None:intergenic 35.0%
GTAACAACATTCTCTACTTG+GGG - chr6_2:58186740-58186759 None:intergenic 35.0%
GGTAACAACATTCTCTACTT+GGG - chr6_2:58186741-58186760 None:intergenic 35.0%
TGGTAACAACATTCTCTACT+TGG - chr6_2:58186742-58186761 None:intergenic 35.0%
TCTTTCTTCACAAATTCCTC+AGG - chr6_2:58186773-58186792 None:intergenic 35.0%
AACGGAGATAGAAATGCAAA+AGG + chr6_2:58186793-58186812 Msa0921230:CDS 35.0%
ACAGATAAGGAAAAAACTCG+AGG + chr6_2:58186865-58186884 Msa0921230:CDS 35.0%
AAGCAGACAATTGCAACTAT+TGG + chr6_2:58186939-58186958 Msa0921230:three_prime_UTR 35.0%
CGAAAGTTAAAAGGTTTGTG+AGG + chr6_2:58186970-58186989 Msa0921230:three_prime_UTR 35.0%
! TTACTTTGGTTCAGTCTTCT+TGG - chr6_2:58187120-58187139 None:intergenic 35.0%
CTAAAAGGAAAAACTACAGC+AGG + chr6_2:58187225-58187244 Msa0921230:intron 35.0%
AAAAGGAAAAACTACAGCAG+GGG + chr6_2:58187227-58187246 Msa0921230:intron 35.0%
GTCATTTCTTCATGTCAAGT+TGG + chr6_2:58187308-58187327 Msa0921230:intron 35.0%
! ATTATATCTTCGGTTCGTCA+AGG + chr6_2:58187411-58187430 Msa0921230:intron 35.0%
TAATGGGAAAAACTACGACA+GGG - chr6_2:58187520-58187539 None:intergenic 35.0%
! ATAATGGGAAAAACTACGAC+AGG - chr6_2:58187521-58187540 None:intergenic 35.0%
GGTCATATTTGGCATAACTA+TGG - chr6_2:58187551-58187570 None:intergenic 35.0%
ATGCCAAATATGACCTTTCT+TGG + chr6_2:58187556-58187575 Msa0921230:intron 35.0%
! CAGAACTACAAGCTTTATCA+GGG + chr6_2:58187730-58187749 Msa0921230:intron 35.0%
GTAGTTGCTTAACCAAAAAG+AGG - chr6_2:58188105-58188124 None:intergenic 35.0%
AAGCAACTACGAGAAGAAAT+AGG + chr6_2:58188115-58188134 Msa0921230:intron 35.0%
AACAGAAGGTGATCTTGAAA+AGG + chr6_2:58188174-58188193 Msa0921230:three_prime_UTR 35.0%
CTTAAAGAGATCAGCCAACA+TGG - chr6_2:58184091-58184110 None:intergenic 40.0%
CTTGAATTGTACTCCCAATG+AGG - chr6_2:58184135-58184154 None:intergenic 40.0%
GTTGTTCCTTAGACAGACAA+AGG + chr6_2:58184234-58184253 Msa0921230:intron 40.0%
ACAAAGGCAGTGTTCATATC+AGG + chr6_2:58184250-58184269 Msa0921230:intron 40.0%
AGAATATGACATGGATCCCT+TGG - chr6_2:58184428-58184447 None:intergenic 40.0%
TCCGTGTCATATTCTCATAC+TGG + chr6_2:58184744-58184763 Msa0921230:intron 40.0%
!! TCAAAGTGGCTTTGAACGAT+AGG - chr6_2:58185256-58185275 None:intergenic 40.0%
AGTGAGTTTGATTCTCGTGT+TGG + chr6_2:58185455-58185474 Msa0921230:intron 40.0%
GTTGTATGCTACAAAGGGTT+GGG - chr6_2:58185491-58185510 None:intergenic 40.0%
TGTTGTATGCTACAAAGGGT+TGG - chr6_2:58185492-58185511 None:intergenic 40.0%
CGCAATGTTGTATGCTACAA+AGG - chr6_2:58185497-58185516 None:intergenic 40.0%
CATGCCAATCGCTAATTATG+CGG + chr6_2:58185577-58185596 Msa0921230:intron 40.0%
GCGGAAAAGACATAATGTAG+GGG + chr6_2:58185596-58185615 Msa0921230:intron 40.0%
CGGAAAAGACATAATGTAGG+GGG + chr6_2:58185597-58185616 Msa0921230:intron 40.0%
ACTCATTACGAGTCACAAAG+GGG - chr6_2:58185639-58185658 None:intergenic 40.0%
CACTCATTACGAGTCACAAA+GGG - chr6_2:58185640-58185659 None:intergenic 40.0%
ACACTCATTACGAGTCACAA+AGG - chr6_2:58185641-58185660 None:intergenic 40.0%
TTGAGCTTTACAGAACAGCA+AGG - chr6_2:58185733-58185752 None:intergenic 40.0%
CTGTTCTGTAAAGCTCAACA+GGG + chr6_2:58185735-58185754 Msa0921230:intron 40.0%
!! TTCAGGTAGGATTGGTTTGA+TGG + chr6_2:58185844-58185863 Msa0921230:intron 40.0%
CTGCTCCATCTTTATATCTC+AGG + chr6_2:58185979-58185998 Msa0921230:intron 40.0%
! CTCAGGCTTTTAGTGATTGT+TGG + chr6_2:58185996-58186015 Msa0921230:intron 40.0%
TGGAAAACTAAGACCTCGTT+TGG - chr6_2:58186395-58186414 None:intergenic 40.0%
TCAAAGATTGGTGAACAGGT+TGG - chr6_2:58186415-58186434 None:intergenic 40.0%
ATAGTTGCTGCAATCCAAAC+AGG - chr6_2:58186480-58186499 None:intergenic 40.0%
CAACACTTGTGAAGCAACTT+CGG + chr6_2:58186505-58186524 Msa0921230:CDS 40.0%
AACACTTGTGAAGCAACTTC+GGG + chr6_2:58186506-58186525 Msa0921230:CDS 40.0%
CAAGAGCTCCTTAGGAAAAA+AGG + chr6_2:58186559-58186578 Msa0921230:CDS 40.0%
!!! TTGCCAAGCCTTTTTTCCTA+AGG - chr6_2:58186570-58186589 None:intergenic 40.0%
GTTCTTACATTCATGACAGC+AGG + chr6_2:58186612-58186631 Msa0921230:CDS 40.0%
TACATTCATGACAGCAGGAT+CGG + chr6_2:58186617-58186636 Msa0921230:CDS 40.0%
!! AAATTCCTCAGGAGTGTCTT+TGG - chr6_2:58186762-58186781 None:intergenic 40.0%
GGATTGTTGAAGGACAGATA+AGG + chr6_2:58186852-58186871 Msa0921230:CDS 40.0%
AGGTTTGTGAGGTTCAATCT+CGG + chr6_2:58186981-58187000 Msa0921230:three_prime_UTR 40.0%
! TTTTTCACTGGAGTCGTTAC+CGG - chr6_2:58187073-58187092 None:intergenic 40.0%
AATGGGAAAAACTACGACAG+GGG - chr6_2:58187519-58187538 None:intergenic 40.0%
CCAAATATGACCTTTCTTGG+CGG + chr6_2:58187559-58187578 Msa0921230:intron 40.0%
TTTAATGCAACCGCCAAGAA+AGG - chr6_2:58187572-58187591 None:intergenic 40.0%
AATGCAACCCATTGCTCAAA+CGG - chr6_2:58187608-58187627 None:intergenic 40.0%
CCTGATAAAGCTTGTAGTTC+TGG - chr6_2:58187732-58187751 None:intergenic 40.0%
! CCAGAACTACAAGCTTTATC+AGG + chr6_2:58187729-58187748 Msa0921230:intron 40.0%
ATAAGTATGGAGGGAAGGTT+CGG - chr6_2:58187782-58187801 None:intergenic 40.0%
AGAAATAGGTGCAGGAATGA+TGG + chr6_2:58188129-58188148 Msa0921230:intron 40.0%
AAAGCTCTTGCTGAAACAGA+AGG + chr6_2:58188160-58188179 Msa0921230:three_prime_UTR 40.0%
GCAAGCATACCTCAGAAAAA+AGG + chr6_2:58188198-58188217 Msa0921230:three_prime_UTR 40.0%
AAGCTCCTTAATGCCCTCAT+TGG + chr6_2:58184118-58184137 Msa0921230:intron 45.0%
AGCTCCTTAATGCCCTCATT+GGG + chr6_2:58184119-58184138 Msa0921230:intron 45.0%
!! TACTCCCAATGAGGGCATTA+AGG - chr6_2:58184126-58184145 None:intergenic 45.0%
ACACTGCCTTTGTCTGTCTA+AGG - chr6_2:58184243-58184262 None:intergenic 45.0%
! TGGTACGTCGATTAAGATGC+AGG + chr6_2:58184331-58184350 Msa0921230:intron 45.0%
! GGTACGTCGATTAAGATGCA+GGG + chr6_2:58184332-58184351 Msa0921230:intron 45.0%
AAGATGCAGGGCTACAAAAC+TGG + chr6_2:58184344-58184363 Msa0921230:intron 45.0%
AGATGCAGGGCTACAAAACT+GGG + chr6_2:58184345-58184364 Msa0921230:intron 45.0%
TTGGTAAGTTTGGCACTCCA+TGG - chr6_2:58184409-58184428 None:intergenic 45.0%
! ATGGAGTGCCAAACTTACCA+AGG + chr6_2:58184408-58184427 Msa0921230:intron 45.0%
! TGGAGTGCCAAACTTACCAA+GGG + chr6_2:58184409-58184428 Msa0921230:intron 45.0%
CATGGATCCCTTGGTAAGTT+TGG - chr6_2:58184419-58184438 None:intergenic 45.0%
AAGATGCAGGGCTACAAAAC+TGG + chr6_2:58184344-58184363 Msa0921230:intron 45.0%
AGATGCAGGGCTACAAAACT+GGG + chr6_2:58184345-58184364 Msa0921230:intron 45.0%
TTGGTAAGTTTGGCACTCCA+TGG - chr6_2:58184409-58184428 None:intergenic 45.0%
! ATGGAGTGCCAAACTTACCA+AGG + chr6_2:58184408-58184427 Msa0921230:intron 45.0%
! TGGAGTGCCAAACTTACCAA+GGG + chr6_2:58184409-58184428 Msa0921230:intron 45.0%
AGAATATGACACGGATCCCT+TGG - chr6_2:58184739-58184758 None:intergenic 45.0%
ACTACTACTTTCAGCCGATG+TGG + chr6_2:58185294-58185313 Msa0921230:intron 45.0%
CTACTACTTTCAGCCGATGT+GGG + chr6_2:58185295-58185314 Msa0921230:intron 45.0%
GCTGTTCTGTAAAGCTCAAC+AGG + chr6_2:58185734-58185753 Msa0921230:intron 45.0%
TGCTAAGGTTCAGGTAGGAT+TGG + chr6_2:58185836-58185855 Msa0921230:intron 45.0%
! AACTTGCTTAGATCCCCTCA+TGG + chr6_2:58186168-58186187 Msa0921230:intron 45.0%
CGAAGTTGCTTCACAAGTGT+TGG - chr6_2:58186507-58186526 None:intergenic 45.0%
GCTCCTTAGGAAAAAAGGCT+TGG + chr6_2:58186564-58186583 Msa0921230:CDS 45.0%
GTGCAGACAAGAAAACAGCT+AGG + chr6_2:58186590-58186609 Msa0921230:CDS 45.0%
TGCTGATATAGCCATGCAAG+TGG + chr6_2:58186706-58186725 Msa0921230:CDS 45.0%
TGATATAGCCATGCAAGTGG+CGG + chr6_2:58186709-58186728 Msa0921230:CDS 45.0%
TGTTACCAAAGACACTCCTG+AGG + chr6_2:58186754-58186773 Msa0921230:CDS 45.0%
GGGTTTGGAAAAGAAAAGCC+AGG + chr6_2:58187007-58187026 Msa0921230:three_prime_UTR 45.0%
CAACTTCAGCAGCAAAATCC+TGG - chr6_2:58187028-58187047 None:intergenic 45.0%
GGTAACGACTCCAGTGAAAA+AGG + chr6_2:58187072-58187091 Msa0921230:three_prime_UTR 45.0%
ACTCCAGTGAAAAAGGAGCT+TGG + chr6_2:58187079-58187098 Msa0921230:three_prime_UTR 45.0%
! CGACAGGGGTCTTTAACTTA+AGG - chr6_2:58187505-58187524 None:intergenic 45.0%
CCGCCAAGAAAGGTCATATT+TGG - chr6_2:58187562-58187581 None:intergenic 45.0%
TGCTAACCCGTTTGAGCAAT+GGG + chr6_2:58187598-58187617 Msa0921230:intron 45.0%
ATGCAACCCATTGCTCAAAC+GGG - chr6_2:58187607-58187626 None:intergenic 45.0%
TAAGTATGGAGGGAAGGTTC+GGG - chr6_2:58187781-58187800 None:intergenic 45.0%
GAACATTTGACGCGACACTA+AGG + chr6_2:58187912-58187931 Msa0921230:intron 45.0%
ACTGAACGTAACCACATGTG+CGG + chr6_2:58187976-58187995 Msa0921230:intron 45.0%
GTTGTGTTGCCGGAAATCAA+CGG + chr6_2:58188067-58188086 Msa0921230:intron 45.0%
!! AGACCACTACCGTTGATTTC+CGG - chr6_2:58188079-58188098 None:intergenic 45.0%
! GAAATCAACGGTAGTGGTCT+CGG + chr6_2:58188079-58188098 Msa0921230:intron 45.0%
CTACGAGAAGAAATAGGTGC+AGG + chr6_2:58188121-58188140 Msa0921230:intron 45.0%
GATGCAGGGCTACAAAACTG+GGG + chr6_2:58184346-58184365 Msa0921230:intron 50.0%
GATGCAGGGCTACAAAACTG+GGG + chr6_2:58184346-58184365 Msa0921230:intron 50.0%
CACGGATCCCTTGGTAAGTT+TGG - chr6_2:58184730-58184749 None:intergenic 50.0%
GTATGCTACAAAGGGTTGGG+TGG - chr6_2:58185488-58185507 None:intergenic 50.0%
ACGTCTGTTCCACTCCTGTT+TGG + chr6_2:58186463-58186482 Msa0921230:intron 50.0%
TGCTGCAATCCAAACAGGAG+TGG - chr6_2:58186475-58186494 None:intergenic 50.0%
CGAAGCAGAAGCTGGAAAGA+TGG + chr6_2:58186530-58186549 Msa0921230:CDS 50.0%
GGACTGTACAAGAGCTCCTT+AGG + chr6_2:58186551-58186570 Msa0921230:CDS 50.0%
GAGACAGATTTCGTCTCCCA+AGG + chr6_2:58186662-58186681 Msa0921230:CDS 50.0%
GGATTTAGCGTTGCTTGAGC+AGG + chr6_2:58186886-58186905 Msa0921230:CDS 50.0%
TGTGAGGTTCAATCTCGGAG+AGG + chr6_2:58186986-58187005 Msa0921230:three_prime_UTR 50.0%
GTGAGGTTCAATCTCGGAGA+GGG + chr6_2:58186987-58187006 Msa0921230:three_prime_UTR 50.0%
GTTCAATCTCGGAGAGGGTT+TGG + chr6_2:58186992-58187011 Msa0921230:three_prime_UTR 50.0%
!! CAGCCAAGCTCCTTTTTCAC+TGG - chr6_2:58187085-58187104 None:intergenic 50.0%
GTGCTAACCCGTTTGAGCAA+TGG + chr6_2:58187597-58187616 Msa0921230:intron 50.0%
CTGAACGTAACCACATGTGC+GGG + chr6_2:58187977-58187996 Msa0921230:intron 50.0%
ACAACTAACACCCGCACATG+TGG - chr6_2:58187990-58188009 None:intergenic 50.0%
TTGCCGGAAATCAACGGTAG+TGG + chr6_2:58188073-58188092 Msa0921230:intron 50.0%
! TAGTGGTCTCGGCCTCTTTT+TGG + chr6_2:58188090-58188109 Msa0921230:intron 50.0%
CTTCGGGACGAAGCAGAAGC+TGG + chr6_2:58186522-58186541 Msa0921230:CDS 60.0%
GGGACATGCCGCCACTTGCA+TGG - chr6_2:58186720-58186739 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 58184078 58188221 58184078 ID=Msa0921230;Name=Msa0921230
chr6_2 mRNA 58184078 58188221 58184078 ID=Msa0921230-mRNA-1;Parent=Msa0921230;Name=Msa0921230-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|0.5|0.66|0.66|0.5|0|3|307|152
chr6_2 exon 58184078 58184115 58184078 ID=Msa0921230-mRNA-1:exon:8658;Parent=Msa0921230-mRNA-1
chr6_2 exon 58186493 58187135 58186493 ID=Msa0921230-mRNA-1:exon:8659;Parent=Msa0921230-mRNA-1
chr6_2 exon 58188137 58188221 58188137 ID=Msa0921230-mRNA-1:exon:8660;Parent=Msa0921230-mRNA-1
chr6_2 CDS 58184078 58184115 58184078 ID=Msa0921230-mRNA-1:cds;Parent=Msa0921230-mRNA-1
chr6_2 CDS 58186493 58186913 58186493 ID=Msa0921230-mRNA-1:cds;Parent=Msa0921230-mRNA-1
chr6_2 three_prime_UTR 58186914 58187135 58186914 ID=Msa0921230-mRNA-1:three_prime_utr;Parent=Msa0921230-mRNA-1
chr6_2 three_prime_UTR 58188137 58188221 58188137 ID=Msa0921230-mRNA-1:three_prime_utr;Parent=Msa0921230-mRNA-1
Gene Sequence

>Msa0921230

TTGAAGACTTCCATGTTGGCTGATCTCTTTAAGACAAACAACTATATCGCCAACACTTGTGAAGCAACTTCGGGACGAAGCAGAAGCTGGAAAGATGGACTGTACAAGAGCTCCTTAGGAAAAAAGGCTTGGCAAGTGCAGACAAGAAAACAGCTAGGTTCTTACATTCATGACAGCAGGATCGGTGTTTTAGTAGAAGTAAACTGCGAGACAGATTTCGTCTCCCAAGGTGAAATTTTCAGAGAGTGTGTTGCTGATATAGCCATGCAAGTGGCGGCATGTCCCCAAGTAGAGAATGTTGTTACCAAAGACACTCCTGAGGAATTTGTGAAGAAAGAAACGGAGATAGAAATGCAAAAGGAAGATCTTGCATCAAAACCGAAACAAAATAGATCAAGGATTGTTGAAGGACAGATAAGGAAAAAACTCGAGGATTTAGCGTTGCTTGAGCAGGATTAA
Protein sequence

>Msa0921230

LKTSMLADLFKTNNYIANTCEATSGRSRSWKDGLYKSSLGKKAWQVQTRKQLGSYIHDSRIGVLVEVNCETDFVSQGEIFRECVADIAMQVAACPQVENVVTKDTPEEFVKKETEIEMQKEDLASKPKQNRSRIVEGQIRKKLEDLALLEQD*