AlfalfaGEDB | Msa0921750

M. sativa cultivar zhongmu-4 / Msa0921750

KEGG: K04733
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0071944 GO:0098588 GO:0098805
PFAM: PF08263.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0921750	XP_013452655.1	97.647	680	12	1	1	680	1	676	0.0	1355

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0921750	sp\|Q9LP77\|Y1848_ARATH	57.846	650	246	11	22	669	32	655	0.0	707

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0895000	Msa0921750	0.858168	9.916677e-63	-8.615850e-47
Msa0921750	Msa0994540	0.808249	3.397678e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0921750	MtrunA17_Chr6g0478621	97.647	680	12	1	1	680	1	676	0.0	1355
Msa0921750	MtrunA17_Chr8g0393211	68.654	654	165	11	25	667	35	659	0.0	829
Msa0921750	MtrunA17_Chr5g0434551	53.461	679	266	11	1	676	1	632	0.0	670
Msa0921750	MtrunA17_Chr3g0108301	43.628	667	314	13	1	655	13	629	9.36e-174	511
Msa0921750	MtrunA17_Chr5g0432851	44.024	661	312	12	1	655	22	630	1.05e-169	500
Msa0921750	MtrunA17_Chr3g0128551	43.769	658	309	15	22	668	28	635	6.19e-164	485
Msa0921750	MtrunA17_Chr8g0387791	44.103	619	283	12	40	654	38	597	5.00e-157	468
Msa0921750	MtrunA17_Chr7g0245171	42.705	658	318	14	3	655	6	609	1.17e-149	447
Msa0921750	MtrunA17_Chr3g0126271	39.344	671	337	10	7	670	6	613	2.14e-148	444
Msa0921750	MtrunA17_Chr1g0209521	44.024	661	311	20	11	653	19	638	2.55e-145	438

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0921750	AT1G48480.1	57.846	650	246	11	22	669	32	655	0.0	707
Msa0921750	AT3G17840.1	57.519	645	230	11	22	655	30	641	0.0	680
Msa0921750	AT5G16590.1	51.064	658	275	14	5	660	9	621	0.0	606
Msa0921750	AT3G02880.1	51.818	660	267	13	2	656	6	619	0.0	603
Msa0921750	AT2G26730.1	47.424	660	307	10	9	667	11	631	1.16e-179	526
Msa0921750	AT3G08680.1	43.314	688	325	16	1	675	2	637	2.33e-166	492
Msa0921750	AT3G08680.2	43.314	688	325	16	1	675	2	637	2.33e-166	492
Msa0921750	AT5G05160.1	43.485	683	320	22	6	676	12	640	3.14e-151	453
Msa0921750	AT1G68400.1	43.818	639	316	19	25	653	32	637	5.95e-145	438
Msa0921750	AT5G24100.1	39.098	665	332	14	3	654	9	613	2.34e-136	414

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Find 251 sgRNAs with CRISPR-Local

Find 341 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTCGCCGGAAGTTCAC+CGG	0.507469	6_2:+59371635	None:intergenic
AAACCCCACCGGAATTTCAC+CGG	0.536957	6_2:+59371563	None:intergenic
AAACTAAAATTATTTGTAAT+TGG	0.109187	6_2:+59369033	None:intergenic
AAATGGTAATGGATATTCGT+CGG	0.657180	6_2:-59371022	Msa0921750:CDS
AAATTATTTAAAAGTTTAGG+AGG	0.508117	6_2:+59369151	None:intergenic
AACCCTTGTCCTAAGGAAGG+AGG	0.657143	6_2:-59371342	Msa0921750:CDS
AACGGAGGCTGAGAGTGTGG+AGG	0.705941	6_2:+59371742	None:intergenic
AACTAAAATTATTTGTAATT+GGG	0.316775	6_2:+59369034	None:intergenic
AAGAAAGGGAAATTATCTGG+TGG	0.682756	6_2:-59371216	Msa0921750:CDS
AAGAAGAAAGGGAAATTATC+TGG	0.385486	6_2:-59371219	Msa0921750:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGATTTGAGAGATAAAAAGA+AGG	+	chr6_2:59368838-59368857	None:intergenic	30.0%
CTTTGTATCTTCAAAACAAC+CGG	-	chr6_2:59369447-59369466	Msa0921750:CDS	30.0%
GTTTCAAACAACATGCTAAA+CGG	-	chr6_2:59369527-59369546	Msa0921750:CDS	30.0%
AAGAAGAAAGGGAAATTATC+TGG	-	chr6_2:59369746-59369765	Msa0921750:CDS	30.0%
CATAATCAGCTTATTGATGA+AGG	-	chr6_2:59369893-59369912	Msa0921750:CDS	30.0%
ATGAAAATTTAGCACCTCTT+AGG	-	chr6_2:59370257-59370276	Msa0921750:intron	30.0%
TGAAAATTTAGCACCTCTTA+GGG	-	chr6_2:59370258-59370277	Msa0921750:intron	30.0%
CTATAATAATAAGCCCTAAG+AGG	+	chr6_2:59370274-59370293	None:intergenic	30.0%
GAAACATGAACAAGCAAAAA+AGG	+	chr6_2:59370386-59370405	None:intergenic	30.0%
TCTTGCATTACATTTCCATT+TGG	-	chr6_2:59370611-59370630	Msa0921750:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	59368740	59372247	59368740	ID=Msa0921750;Name=Msa0921750
chr6_2	mRNA	59368740	59372247	59368740	ID=Msa0921750-mRNA-1;Parent=Msa0921750;Name=Msa0921750-mRNA-1;_AED=0.00;_eAED=0.00;_QI=214\|1\|1\|1\|1\|1\|2\|704\|680
chr6_2	exon	59368740	59370081	59368740	ID=Msa0921750-mRNA-1:exon:9048;Parent=Msa0921750-mRNA-1
chr6_2	exon	59370629	59372247	59370629	ID=Msa0921750-mRNA-1:exon:9047;Parent=Msa0921750-mRNA-1
chr6_2	five_prime_UTR	59372034	59372247	59372034	ID=Msa0921750-mRNA-1:five_prime_utr;Parent=Msa0921750-mRNA-1
chr6_2	CDS	59370629	59372033	59370629	ID=Msa0921750-mRNA-1:cds;Parent=Msa0921750-mRNA-1
chr6_2	CDS	59369444	59370081	59369444	ID=Msa0921750-mRNA-1:cds;Parent=Msa0921750-mRNA-1
chr6_2	three_prime_UTR	59368740	59369443	59368740	ID=Msa0921750-mRNA-1:three_prime_utr;Parent=Msa0921750-mRNA-1

Gene Sequence

>Msa0921750

ATGAAGTTTTATTTCTCATTCTTTTTCTTTTTTCAGACACTTTTTCTTCCGTCGGTAAAACCCGACCTCACTTCCGAACGCGCCGCTTTATTAACCCTACGCGCCTCCGTAACCGGCCGAACTCTACTCTGGAACACTACCTCCGCCTCTCCCTGCAACTGGGCCGGCGTCCATTGCAACCAAAACCACACTCACGTTGTCGAGCTTCATCTCCCCGCCGTCGCACTCTCCGGCAACCTCCCCACCGGAGTATTCTCCGAACTCCCTAACCTCCACACTCTCAGCCTCCGTTTCAACTCTCTCTCCGGTCCACTCCCCTCCGACCTTGCTTCTTGCACCTCCCTCAAAAACCTCTACCTTCAGCAGAATCTTCTCTCCGGTGAACTTCCGGCGACTTTTTTCAACCTCACCGGATTAGTTCGTCTGAACTTAGCTTCCAACAATTTCTCCGGTGAAATTCCGGTGGGGTTTGGTAACCTAACCCGGTTGAAAACTTTGTATCTTCAAAACAACCGGTTCACCGGTTCGTTAAGCGAGTTTGAGTTAAACCCGGTTCAACTCGCTCAGTTCAATGTTTCAAACAACATGCTAAACGGTTCTGTTCCTGAAAAGTTGCAAACTTTTGGTAAAGATTCATTTTTGGGTAATTTGTTATGTGGGAAACCACTTAACCCTTGTCCTAAGGAAGGAGGTGCAAATAGTGGAAAAGGGGGTGGAAATGGGGGTGCAAATAGTGGAAATGGGGGTAGAAATTCAAGTGTTGTTGATGAAAATAATGGGTTTGTGAGGAACAAGAAGAAAGGGAAATTATCTGGTGGTGCTATAGCTGGAATTGTTATTGGGTCAGTTGTGATTCTTTTGCTTGTGGTTTTTGCTTTGATTGTTTTATGTAGGAATAGAAATGGTGAAAAAATTGATGAAGTTGCAAGAACTTTGAAGCATAATCAGCTTATTGATGAAGGGATTCATGGTGAGAATGTTGGTCATGGAAATGGTAATGGATATTCGTCGGCCGCGGCTATGGCGGTTGCGGCGCCGGTGAATGGTGAGAGAGGTAATGAAGGTGTTGTGATTGGTGGTGGTGATAAAAAATTGGTATTTTTTAAGAATTGGGGTAGGGTGTTTGATTTAGAGGATTTGTTAAGGGCTTCTGCTGAGGTATTAGGGAAAGGGACATTTGGGACATCTTACAAGGCAGTTATGGAGGCTGGTCCTGTGGCTGTGGCTGTGAAGAGGTTAAGGGATGTAACAATTTCTGAGAGGGAGTTTAAGGAGAAGATTGAGAAGGTTGGAACAATGGTTCATGAAAATTTAGCACCTCTTAGGGCTTATTATTATAGTAGAGATGAGAAGCTTCTTGTTCATGATTATTTGCATATGGGAAGTTTATCTGCACTTTTGCATGGAAACAGAGGAGGGGGTAGGACACCATTGACTTGGGAAATGAGATCAGGCATTGCACTTGGAGCCGCTCGCGGCATTGAATACCTACATTCACAAGGTCCTAATGTTTCTCATGGAAACATAAAGTCATCTAACATCCTACTAACCAAGTCCTACGATGCAAGAGTGTCTGATTTCGGTCTTGCACATCTCGTTGGTCCTTCATCCACACCCAATAGGGTTGCTGGCTACCGTGCTCCCGAGGTAACCGATCCTCGGAAAATTTCTCAAAAGGCAGATGTATACAGCTTTGGTGTATTGCTCTTGGAACTTCTGACCGGAAAGGCTCCAACTCACGCCCTCTTGAACGACGAAGGTGTGGACCTTCCTCGATGGGTGCAATCGGTTGTTAAAGAAGAATGGACTTCTGAGGTCTTTGATCTTGAACTTCTTAGGTATCAAAATGTTGAAGAGGAAATGGTTCAGTTGTTGCAACTTGCCGTAGATTGTGCTGCTCCATACCCCGATAACCGCCCTTCCATGTCGCAGGTGAGACAGCACATAGAAGAGTTGCATAGGTCTAGCTTGAAAGAGGGTAGTCAAGACCAAATTCAACAACCTGATGTGATCAGTGACATAGATGATATCTCTTCTAGATGA

Protein sequence

>Msa0921750

MKFYFSFFFFFQTLFLPSVKPDLTSERAALLTLRASVTGRTLLWNTTSASPCNWAGVHCNQNHTHVVELHLPAVALSGNLPTGVFSELPNLHTLSLRFNSLSGPLPSDLASCTSLKNLYLQQNLLSGELPATFFNLTGLVRLNLASNNFSGEIPVGFGNLTRLKTLYLQNNRFTGSLSEFELNPVQLAQFNVSNNMLNGSVPEKLQTFGKDSFLGNLLCGKPLNPCPKEGGANSGKGGGNGGANSGNGGRNSSVVDENNGFVRNKKKGKLSGGAIAGIVIGSVVILLLVVFALIVLCRNRNGEKIDEVARTLKHNQLIDEGIHGENVGHGNGNGYSSAAAMAVAAPVNGERGNEGVVIGGGDKKLVFFKNWGRVFDLEDLLRASAEVLGKGTFGTSYKAVMEAGPVAVAVKRLRDVTISEREFKEKIEKVGTMVHENLAPLRAYYYSRDEKLLVHDYLHMGSLSALLHGNRGGGRTPLTWEMRSGIALGAARGIEYLHSQGPNVSHGNIKSSNILLTKSYDARVSDFGLAHLVGPSSTPNRVAGYRAPEVTDPRKISQKADVYSFGVLLLELLTGKAPTHALLNDEGVDLPRWVQSVVKEEWTSEVFDLELLRYQNVEEEMVQLLQLAVDCAAPYPDNRPSMSQVRQHIEELHRSSLKEGSQDQIQQPDVISDIDDISSR*