Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0927440 | RHN52877.1 | 95.000 | 60 | 3 | 0 | 1 | 60 | 4 | 63 | 2.01e-32 | 116 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0927440 | sp|Q9FK16|AGP22_ARATH | 50.000 | 56 | 23 | 1 | 5 | 60 | 13 | 63 | 6.61e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0899400 | Msa0927440 | 0.887564 | 1.276982e-72 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0927440 | MtrunA17_Chr6g0485321 | 95.000 | 60 | 3 | 0 | 1 | 60 | 4 | 63 | 1.85e-36 | 116 |
| Msa0927440 | MtrunA17_Chr6g0485341 | 91.667 | 60 | 5 | 0 | 1 | 60 | 4 | 63 | 2.22e-34 | 110 |
| Msa0927440 | MtrunA17_Chr6g0485351 | 76.667 | 60 | 14 | 0 | 1 | 60 | 5 | 64 | 4.44e-29 | 97.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0927440 | AT5G53250.1 | 50.000 | 56 | 23 | 1 | 5 | 60 | 13 | 63 | 6.72e-12 | 54.7 |
| Msa0927440 | AT3G61640.1 | 45.763 | 59 | 30 | 1 | 2 | 60 | 6 | 62 | 1.51e-11 | 53.9 |
| Msa0927440 | AT2G46330.1 | 49.091 | 55 | 26 | 1 | 6 | 60 | 10 | 62 | 1.83e-11 | 53.9 |
Find 12 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACTAGTGATGCTCAGATTC+TGG | 0.273706 | 6_2:+71819356 | Msa0927440:CDS |
| TTCTCTTATGTTGGTGGCGC+TGG | 0.309056 | 6_2:+71819395 | Msa0927440:CDS |
| TGCTTATTCTCTTATGTTGG+TGG | 0.427507 | 6_2:+71819389 | Msa0927440:CDS |
| GTTCTTGGCAATTTCTCACA+TGG | 0.459379 | 6_2:+71819293 | Msa0927440:CDS |
| AAGAACAAAATAGTAAGAAG+TGG | 0.472674 | 6_2:-71819276 | None:intergenic |
| AATTGCTTATTCTCTTATGT+TGG | 0.478265 | 6_2:+71819386 | Msa0927440:CDS |
| AGAATCTGAGCATCACTAGT+AGG | 0.516211 | 6_2:-71819354 | None:intergenic |
| TTCTTGGCAATTTCTCACAT+GGG | 0.525548 | 6_2:+71819294 | Msa0927440:CDS |
| TCACTAGTAGGAGTAGGTGC+TGG | 0.561134 | 6_2:-71819342 | None:intergenic |
| AGAACAAAATAGTAAGAAGT+GGG | 0.592067 | 6_2:-71819275 | None:intergenic |
| GATGCTCAGATTCTGGATCA+AGG | 0.592593 | 6_2:+71819363 | Msa0927440:CDS |
| TGAGCATCACTAGTAGGAGT+AGG | 0.610713 | 6_2:-71819348 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAACAAAATAGTAAGAAGT+GGG | - | chr6_2:71819278-71819297 | None:intergenic | 25.0% |
| ! | AAGAACAAAATAGTAAGAAG+TGG | - | chr6_2:71819279-71819298 | None:intergenic | 25.0% |
| !! | ACTTCTTACTATTTTGTTCT+TGG | + | chr6_2:71819278-71819297 | Msa0927440:CDS | 25.0% |
| ! | AATTGCTTATTCTCTTATGT+TGG | + | chr6_2:71819386-71819405 | Msa0927440:CDS | 25.0% |
| TTCTTGGCAATTTCTCACAT+GGG | + | chr6_2:71819294-71819313 | Msa0927440:CDS | 35.0% | |
| TGCTTATTCTCTTATGTTGG+TGG | + | chr6_2:71819389-71819408 | Msa0927440:CDS | 35.0% | |
| GTTCTTGGCAATTTCTCACA+TGG | + | chr6_2:71819293-71819312 | Msa0927440:CDS | 40.0% | |
| AGAATCTGAGCATCACTAGT+AGG | - | chr6_2:71819357-71819376 | None:intergenic | 40.0% | |
| TACTAGTGATGCTCAGATTC+TGG | + | chr6_2:71819356-71819375 | Msa0927440:CDS | 40.0% | |
| TGAGCATCACTAGTAGGAGT+AGG | - | chr6_2:71819351-71819370 | None:intergenic | 45.0% | |
| GATGCTCAGATTCTGGATCA+AGG | + | chr6_2:71819363-71819382 | Msa0927440:CDS | 45.0% | |
| TCACTAGTAGGAGTAGGTGC+TGG | - | chr6_2:71819345-71819364 | None:intergenic | 50.0% | |
| !! | TTCTCTTATGTTGGTGGCGC+TGG | + | chr6_2:71819395-71819414 | Msa0927440:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 71819258 | 71819440 | 71819258 | ID=Msa0927440;Name=Msa0927440 |
| chr6_2 | mRNA | 71819258 | 71819440 | 71819258 | ID=Msa0927440-mRNA-1;Parent=Msa0927440;Name=Msa0927440-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|60 |
| chr6_2 | exon | 71819258 | 71819440 | 71819258 | ID=Msa0927440-mRNA-1:exon:12052;Parent=Msa0927440-mRNA-1 |
| chr6_2 | CDS | 71819258 | 71819440 | 71819258 | ID=Msa0927440-mRNA-1:cds;Parent=Msa0927440-mRNA-1 |
| Gene Sequence |
| Protein sequence |
