AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0929910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0929910 MtrunA17_Chr8g0392931 98.174 219 2 2 1 218 1 218 1.88e-156 432
Msa0929910 MtrunA17_Chr6g0482721 77.725 211 47 0 8 218 5 215 5.68e-122 344
Msa0929910 MtrunA17_Chr4g0046261 58.019 212 85 2 9 218 7 216 1.25e-84 250
Msa0929910 MtrunA17_Chr2g0283131 57.075 212 87 2 9 218 7 216 6.52e-84 248
Msa0929910 MtrunA17_Chr4g0040781 57.075 212 87 2 9 218 7 216 7.68e-84 248
Msa0929910 MtrunA17_Chr2g0325091 56.398 211 89 2 9 218 5 213 2.92e-82 244
Msa0929910 MtrunA17_Chr2g0315551 55.714 210 93 0 9 218 7 216 5.46e-81 241
Msa0929910 MtrunA17_Chr3g0112681 53.810 210 97 0 9 218 6 215 1.23e-79 237
Msa0929910 MtrunA17_Chr2g0276941 54.460 213 96 1 7 218 7 219 1.65e-79 237
Msa0929910 MtrunA17_Chr4g0032201 54.286 210 95 1 9 218 6 214 6.68e-79 235
Msa0929910 MtrunA17_Chr3g0123821 52.804 214 94 3 9 217 10 221 2.11e-76 229
Msa0929910 MtrunA17_Chr2g0319951 53.555 211 97 1 9 218 7 217 2.63e-76 229
Msa0929910 MtrunA17_Chr8g0381841 53.241 216 91 4 9 217 11 223 1.71e-74 224
Msa0929910 MtrunA17_Chr7g0231131 49.528 212 105 1 8 217 8 219 6.42e-73 220
Msa0929910 MtrunA17_Chr7g0275991 51.174 213 100 1 10 218 15 227 6.98e-73 220
Msa0929910 MtrunA17_Chr1g0174281 47.907 215 107 2 8 217 8 222 3.93e-72 218
Msa0929910 MtrunA17_Chr1g0189361 53.052 213 92 3 13 218 12 223 5.62e-72 218
Msa0929910 MtrunA17_Chr7g0214241 52.153 209 94 2 14 218 14 220 7.81e-71 215
Msa0929910 MtrunA17_Chr8g0346421 48.214 224 104 3 3 218 12 231 5.26e-69 211
Msa0929910 MtrunA17_Chr7g0234201 52.113 213 85 3 14 218 12 215 4.42e-67 205
Msa0929910 MtrunA17_Chr8g0351861 46.903 226 106 4 1 218 8 227 2.37e-65 201
Msa0929910 MtrunA17_Chr7g0250531 46.919 211 102 2 8 218 1 201 2.19e-59 185
Msa0929910 MtrunA17_Chr4g0055651 47.642 212 99 4 8 218 1 201 1.51e-57 181
Msa0929910 MtrunA17_Chr2g0290891 50.847 177 79 1 14 182 5 181 5.44e-56 177
Msa0929910 MtrunA17_Chr6g0458081 44.712 208 113 1 13 218 4 211 6.99e-56 177
Msa0929910 MtrunA17_Chr2g0276931 51.807 166 80 0 11 176 5 170 1.87e-54 172
Msa0929910 MtrunA17_Chr8g0335471 45.755 212 104 3 8 218 1 202 8.12e-54 171
Msa0929910 MtrunA17_Chr8g0349711 48.603 179 91 1 14 192 5 182 9.57e-54 171
Msa0929910 MtrunA17_Chr1g0212521 43.204 206 112 2 13 217 13 214 6.35e-52 167
Msa0929910 MtrunA17_Chr1g0182321 43.689 206 111 2 13 217 13 214 6.40e-52 167
Msa0929910 MtrunA17_Chr1g0182301 43.204 206 112 2 13 217 13 214 1.81e-51 166
Msa0929910 MtrunA17_Chr8g0349681 51.829 164 79 0 14 177 383 546 2.49e-51 174
Msa0929910 MtrunA17_Chr8g0349681 44.693 179 98 1 14 192 174 351 7.15e-47 162
Msa0929910 MtrunA17_Chr8g0349681 48.428 159 82 0 14 172 12 170 3.48e-45 158
Msa0929910 MtrunA17_Chr4g0030911 44.175 206 109 3 13 217 13 213 1.26e-50 163
Msa0929910 MtrunA17_Chr7g0263071 44.175 206 110 2 13 217 13 214 1.33e-50 163
Msa0929910 MtrunA17_Chr2g0327911 41.232 211 115 5 9 217 9 212 4.28e-47 154
Msa0929910 MtrunA17_Chr8g0384311 45.614 171 90 3 10 178 8 177 1.26e-45 150
Msa0929910 MtrunA17_Chr1g0159481 47.059 170 87 3 11 178 13 181 1.55e-45 150
Msa0929910 MtrunA17_Chr3g0109721 39.604 202 109 2 17 218 12 200 1.17e-44 147
Msa0929910 MtrunA17_Chr5g0433561 38.614 202 111 2 17 218 12 200 2.10e-43 144
Msa0929910 MtrunA17_Chr3g0130271 44.767 172 91 4 10 178 7 177 4.08e-42 141
Msa0929910 MtrunA17_Chr1g0197331 42.473 186 104 3 1 183 1 186 3.66e-41 139
Msa0929910 MtrunA17_Chr3g0123971 36.364 176 109 1 16 191 10 182 1.26e-40 137
Msa0929910 MtrunA17_Chr8g0382221 35.912 181 115 1 16 196 10 189 1.81e-40 137
Msa0929910 MtrunA17_Chr7g0265001 41.346 208 113 4 13 218 14 214 2.37e-40 137
Msa0929910 MtrunA17_Chr1g0199331 45.455 154 83 1 17 169 33 186 2.86e-40 136
Msa0929910 MtrunA17_Chr4g0055081 36.943 157 99 0 16 172 10 166 4.45e-38 131
Msa0929910 MtrunA17_Chr5g0400131 37.662 154 96 0 16 169 10 163 7.35e-38 130
Msa0929910 MtrunA17_Chr1g0180291 43.275 171 94 3 13 181 12 181 2.51e-37 129
Msa0929910 MtrunA17_Chr6g0466301 36.022 186 110 4 15 193 8 191 2.19e-36 127
Msa0929910 MtrunA17_Chr6g0465491 36.022 186 110 4 15 193 8 191 2.19e-36 127
Msa0929910 MtrunA17_Chr8g0390561 35.838 173 111 0 17 189 11 183 9.16e-36 125
Msa0929910 MtrunA17_Chr4g0034871 37.654 162 94 3 15 169 8 169 3.02e-35 124
Msa0929910 MtrunA17_Chr2g0331691 38.889 162 92 3 15 169 10 171 8.89e-35 122
Msa0929910 MtrunA17_Chr1g0201121 35.484 186 111 4 15 193 8 191 4.08e-34 120
Msa0929910 MtrunA17_Chr4g0004661 38.272 162 93 3 15 169 64 225 8.33e-34 121
Msa0929910 MtrunA17_Chr8g0383261 37.423 163 95 3 15 170 12 174 1.89e-33 119
Msa0929910 MtrunA17_Chr6g0466161 33.696 184 112 5 30 205 5 186 2.73e-26 100
Msa0929910 MtrunA17_Chr6g0465471 35.404 161 95 4 30 183 5 163 1.01e-25 99.4
Msa0929910 MtrunA17_Chr3g0138101 30.198 202 134 5 16 214 14 211 2.44e-24 95.9
Msa0929910 MtrunA17_Chr3g0138121 34.395 157 100 3 16 172 14 167 3.14e-24 95.9
Msa0929910 MtrunA17_Chr3g0138111 34.395 157 100 3 16 172 14 167 3.49e-24 95.5
Msa0929910 MtrunA17_Chr2g0325101 31.737 167 100 5 15 169 6 170 5.62e-21 86.7
Msa0929910 MtrunA17_Chr4g0037021 30.108 186 116 5 15 188 6 189 8.06e-20 83.6
Msa0929910 MtrunA17_Chr6g0486041 31.016 187 113 6 15 188 6 189 9.10e-20 83.6
Msa0929910 MtrunA17_Chr8g0371501 31.138 167 101 5 15 169 6 170 2.55e-19 82.0
Msa0929910 MtrunA17_Chr5g0405791 30.539 167 102 5 15 169 6 170 2.55e-19 82.0
Msa0929910 MtrunA17_Chr4g0046211 33.065 124 80 3 15 137 6 127 4.44e-18 77.8
Msa0929910 MtrunA17_Chr3g0117931 32.335 167 99 5 15 169 8 172 9.76e-18 78.2
Msa0929910 MtrunA17_Chr6g0466431 35.644 101 56 1 15 115 8 99 1.38e-16 72.4
Msa0929910 MtrunA17_Chr6g0465611 35.644 101 56 1 15 115 8 99 1.38e-16 72.4
Msa0929910 MtrunA17_Chr1g0178291 36.000 150 89 4 20 169 433 575 1.13e-15 75.1
Msa0929910 MtrunA17_Chr2g0331841 46.552 58 31 0 84 141 2 59 9.47e-13 62.0
Msa0929910 MtrunA17_Chr5g0443631 24.194 186 135 2 16 196 105 289 8.11e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0929910 AT1G18200.1 65.766 222 64 3 8 217 6 227 6.04e-98 285
Msa0929910 AT1G73640.1 64.159 226 65 4 8 217 6 231 4.00e-96 280
Msa0929910 AT5G60860.1 57.075 212 87 2 9 218 7 216 2.22e-84 249
Msa0929910 AT4G18430.1 58.019 212 85 2 9 218 7 216 3.29e-84 249
Msa0929910 AT5G45750.1 59.048 210 85 1 9 218 7 215 5.26e-84 249
Msa0929910 AT1G09630.1 54.762 210 95 0 9 218 6 215 3.20e-83 246
Msa0929910 AT1G16920.1 57.143 210 89 1 9 218 7 215 8.54e-83 246
Msa0929910 AT4G18800.1 57.619 210 86 2 9 218 7 213 2.78e-82 244
Msa0929910 AT2G33870.1 55.660 212 91 2 9 218 7 217 1.53e-81 243
Msa0929910 AT3G15060.1 56.132 212 89 2 9 218 7 216 1.65e-81 242
Msa0929910 AT1G06400.1 57.143 210 89 1 9 218 7 215 3.87e-81 241
Msa0929910 AT1G28550.1 54.717 212 93 2 9 218 7 217 8.16e-80 238
Msa0929910 AT1G07410.1 55.189 212 91 3 8 218 5 213 1.84e-78 234
Msa0929910 AT5G65270.1 53.488 215 93 3 10 218 12 225 1.88e-76 230
Msa0929910 AT3G12160.1 51.887 212 100 1 8 217 8 219 3.47e-76 229
Msa0929910 AT5G47520.1 54.930 213 93 2 9 218 8 220 6.67e-76 228
Msa0929910 AT4G39990.1 53.555 211 94 2 11 218 13 222 6.82e-76 228
Msa0929910 AT5G47960.1 53.052 213 97 1 8 217 8 220 6.18e-75 226
Msa0929910 AT3G46830.1 53.555 211 97 1 9 218 6 216 2.30e-73 222
Msa0929910 AT3G46830.2 53.555 211 97 1 9 218 6 216 2.30e-73 222
Msa0929910 AT2G31680.1 52.632 209 95 2 13 218 10 217 5.20e-72 218
Msa0929910 AT5G59150.1 52.358 212 98 2 9 218 6 216 6.91e-72 218
Msa0929910 AT1G05810.1 53.081 211 90 3 13 218 10 216 7.21e-71 215
Msa0929910 AT1G05810.2 53.081 211 90 3 13 218 56 262 1.83e-70 216
Msa0929910 AT2G43130.1 51.942 206 97 1 13 218 10 213 1.22e-68 209
Msa0929910 AT3G07410.1 52.174 207 98 1 13 218 10 216 1.81e-67 207
Msa0929910 AT1G01200.1 48.165 218 100 4 10 218 23 236 1.17e-64 200
Msa0929910 AT4G35860.1 44.231 208 114 1 13 218 4 211 2.22e-56 178
Msa0929910 AT4G17170.1 48.387 186 96 0 14 199 5 190 3.74e-56 177
Msa0929910 AT4G17530.1 45.498 211 105 2 8 218 1 201 4.25e-56 177
Msa0929910 AT1G02130.1 46.226 212 103 3 8 218 1 202 4.43e-56 177
Msa0929910 AT4G17160.1 49.438 178 86 1 14 191 5 178 1.63e-54 173
Msa0929910 AT5G47200.1 44.550 211 107 2 8 218 1 201 5.03e-54 172
Msa0929910 AT5G59840.1 44.660 206 109 2 13 217 13 214 3.63e-53 170
Msa0929910 AT3G53610.2 44.660 206 109 2 13 217 13 214 5.25e-53 170
Msa0929910 AT3G53610.3 44.660 206 109 2 13 217 13 214 5.25e-53 170
Msa0929910 AT3G53610.1 44.660 206 109 2 13 217 13 214 5.25e-53 170
Msa0929910 AT3G46060.2 44.660 206 109 2 13 217 13 214 4.00e-52 167
Msa0929910 AT3G46060.3 44.660 206 109 2 13 217 13 214 4.00e-52 167
Msa0929910 AT3G46060.1 44.660 206 109 2 13 217 13 214 4.00e-52 167
Msa0929910 AT5G03520.1 45.894 207 106 3 13 218 13 214 3.91e-51 165
Msa0929910 AT3G11730.1 52.469 162 77 0 11 172 4 165 4.58e-51 164
Msa0929910 AT3G09900.1 44.231 208 110 3 13 218 13 216 4.09e-50 162
Msa0929910 AT5G59150.2 48.171 164 82 2 57 218 3 165 1.92e-46 151
Msa0929910 AT4G19640.1 44.872 156 86 0 17 172 12 167 4.80e-45 149
Msa0929910 AT1G43890.3 46.471 170 88 3 11 178 9 177 1.26e-44 148
Msa0929910 AT1G43890.1 46.471 170 88 3 11 178 9 177 1.26e-44 148
Msa0929910 AT1G43890.2 46.471 170 88 3 11 178 9 177 1.26e-44 148
Msa0929910 AT5G45130.1 45.513 156 85 0 17 172 12 167 3.88e-44 147
Msa0929910 AT5G45130.2 45.455 154 84 0 19 172 22 175 2.15e-43 145
Msa0929910 AT3G54840.2 46.753 154 81 1 17 169 35 188 9.19e-42 140
Msa0929910 AT3G54840.1 46.753 154 81 1 17 169 35 188 1.51e-41 140
Msa0929910 AT3G54840.3 46.711 152 80 1 19 169 3 154 1.54e-41 139
Msa0929910 AT4G39890.1 36.723 177 110 2 16 191 10 185 1.11e-40 138
Msa0929910 AT5G03530.2 41.827 208 111 5 13 218 11 210 2.08e-40 137
Msa0929910 AT5G03530.1 41.827 208 111 5 13 218 11 210 2.08e-40 137
Msa0929910 AT4G35860.2 41.718 163 93 1 57 217 2 164 4.17e-40 135
Msa0929910 AT2G44610.1 34.483 203 128 2 16 218 10 207 3.28e-39 134
Msa0929910 AT5G10260.1 37.580 157 98 0 16 172 10 166 4.75e-39 134
Msa0929910 AT2G22290.1 37.580 157 98 0 16 172 10 166 8.54e-38 130
Msa0929910 AT5G03520.2 42.353 170 92 3 50 218 40 204 9.45e-38 130
Msa0929910 AT5G64990.1 32.836 201 131 2 16 216 8 204 1.25e-36 127
Msa0929910 AT4G19640.3 45.038 131 72 0 17 147 12 142 1.91e-36 127
Msa0929910 AT4G19640.2 45.238 126 69 0 17 142 12 137 1.34e-35 123
Msa0929910 AT1G22740.1 39.106 179 100 5 15 186 8 184 2.06e-35 124
Msa0929910 AT3G18820.1 38.889 162 92 3 15 169 8 169 4.00e-35 124
Msa0929910 AT1G49300.2 37.725 167 97 3 15 174 8 174 1.01e-34 122
Msa0929910 AT1G49300.1 37.725 167 97 3 15 174 8 174 1.01e-34 122
Msa0929910 AT5G64990.2 31.731 208 131 3 16 216 8 211 7.75e-34 120
Msa0929910 AT1G52280.1 37.037 162 95 3 15 169 8 169 8.71e-34 120
Msa0929910 AT3G09910.3 42.105 171 96 3 13 181 11 180 4.29e-33 118
Msa0929910 AT3G09910.1 42.105 171 96 3 13 181 11 180 4.29e-33 118
Msa0929910 AT3G09910.2 42.105 171 96 3 13 181 11 180 4.29e-33 118
Msa0929910 AT4G09720.1 34.375 192 113 4 15 193 8 199 8.38e-33 118
Msa0929910 AT2G21880.1 35.758 165 99 3 15 172 9 173 9.99e-33 117
Msa0929910 AT3G16100.1 37.037 162 95 3 15 169 8 169 1.30e-32 117
Msa0929910 AT3G09910.5 43.827 162 88 3 13 172 11 171 2.42e-32 116
Msa0929910 AT3G09910.4 43.827 162 88 3 13 172 11 171 2.42e-32 116
Msa0929910 AT4G09720.4 34.010 197 112 5 15 193 8 204 1.18e-31 115
Msa0929910 AT4G09720.3 32.512 203 113 4 15 193 8 210 2.39e-31 114
Msa0929910 AT5G39620.1 35.955 178 105 4 16 186 6 181 5.18e-31 113
Msa0929910 AT5G46025.1 47.664 107 54 2 77 181 6 112 2.85e-27 100
Msa0929910 AT5G55080.1 31.677 161 107 3 16 176 14 171 1.04e-25 100
Msa0929910 AT2G21880.4 33.108 148 92 3 32 172 1 148 2.17e-24 95.5
Msa0929910 AT2G21880.3 33.108 148 92 3 32 172 1 148 2.17e-24 95.5
Msa0929910 AT5G20010.1 32.292 192 122 4 16 207 14 197 2.35e-24 96.3
Msa0929910 AT5G20020.1 34.395 157 100 3 16 172 14 167 4.34e-24 95.9
Msa0929910 AT5G55190.1 34.395 157 100 3 16 172 14 167 4.62e-24 95.5
Msa0929910 AT2G21880.2 30.909 165 99 4 15 172 9 165 5.54e-23 92.4
Msa0929910 AT4G09720.6 33.951 162 94 4 45 193 4 165 6.67e-23 91.3
Msa0929910 AT4G09720.5 33.951 162 94 4 45 193 4 165 6.67e-23 91.3
Msa0929910 AT4G09720.2 33.951 162 94 4 45 193 4 165 6.67e-23 91.3
Msa0929910 AT4G35020.1 32.571 175 104 5 15 177 6 178 1.91e-20 85.5
Msa0929910 AT4G35020.2 32.571 175 104 5 15 177 6 178 1.91e-20 85.5
Msa0929910 AT4G35020.3 32.571 175 104 5 15 177 6 178 1.91e-20 85.5
Msa0929910 AT2G44690.1 30.286 175 108 4 15 177 18 190 1.87e-19 83.2
Msa0929910 AT4G28950.1 27.933 179 117 4 15 183 6 182 1.19e-18 81.3
Msa0929910 AT3G51300.1 30.303 165 101 5 17 169 8 170 2.78e-18 79.7
Msa0929910 AT2G17800.2 29.940 167 103 5 15 169 6 170 3.38e-18 79.7
Msa0929910 AT2G17800.1 29.940 167 103 5 15 169 6 170 3.38e-18 79.7
Msa0929910 AT4G35950.1 30.769 169 99 5 15 169 6 170 3.79e-18 79.3
Msa0929910 AT1G75840.1 29.143 175 110 5 15 177 6 178 4.98e-18 79.0
Msa0929910 AT5G45970.1 29.940 167 103 5 15 169 6 170 5.01e-18 79.3
Msa0929910 AT1G20090.1 28.571 175 111 5 15 177 5 177 7.88e-18 78.6
Msa0929910 AT3G48040.2 35.484 124 77 3 15 137 8 129 1.35e-17 77.4
Msa0929910 AT3G48040.1 35.484 124 77 3 15 137 8 129 1.62e-17 78.2
Msa0929910 AT5G62880.2 34.677 124 78 3 15 137 8 129 5.46e-17 76.3
Msa0929910 AT5G62880.1 30.539 167 102 5 15 169 8 172 6.15e-17 76.6
Msa0929910 AT4G35020.4 37.719 114 68 3 17 129 8 119 4.16e-16 72.8
Msa0929910 AT4G35020.5 37.719 114 68 3 17 129 8 119 4.16e-16 72.8

Find 51 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGGTGTCTCATCCATTT+TGG 0.207851 6_2:-78727872 None:intergenic
AACTCATCAAAGCTCAAATA+TGG 0.208455 6_2:+78725813 Msa0929910:CDS
TTCCAAAGAGAAGGTTATTC+TGG 0.244361 6_2:-78725553 None:intergenic
TTCCCGTTAGATAGATTAAT+TGG 0.245574 6_2:-78727889 None:intergenic
GGAAGGGAAGTTACCTTTCT+TGG 0.267002 6_2:-78725847 None:intergenic
GGTTTGCAAAAGATGAATTC+AGG 0.331846 6_2:+78725729 Msa0929910:CDS
GAGAAGTTTGGATGCCAAAA+TGG 0.334979 6_2:+78727858 Msa0929910:CDS
GTCCAGAATAACCTTCTCTT+TGG 0.334985 6_2:+78725551 Msa0929910:five_prime_UTR
GCAGTGCTGATTGGTGATTC+TGG 0.349436 6_2:+78725680 Msa0929910:CDS
GGAAATCAAATCTGCTTTCA+AGG 0.357132 6_2:+78725708 Msa0929910:CDS
CATCACAAACCAGAGAAGTT+TGG 0.359508 6_2:+78727846 Msa0929910:CDS
TCATGATCGATGAGAGGTTT+TGG 0.386540 6_2:+78727964 Msa0929910:exon
TGAAGTTACCGCAACTAAAC+AGG 0.414506 6_2:+78727930 Msa0929910:CDS
TTCGTATTATAGAGGAGCCT+TGG 0.423636 6_2:+78727558 Msa0929910:CDS
GCAAATTCAACTCCTATAGT+TGG 0.428034 6_2:-78725764 None:intergenic
GAGATGGTAGTTATTCTAGT+TGG 0.430677 6_2:+78727670 Msa0929910:CDS
TTAGTGGAGCTACGAGAGTT+TGG 0.450057 6_2:+78727640 Msa0929910:CDS
CACCAATTAATCTATCTAAC+GGG 0.454761 6_2:+78727887 Msa0929910:CDS
TTGGATTCAAAGCCAACTAT+AGG 0.457514 6_2:+78725752 Msa0929910:CDS
AGAGTGTGATTACTTGTTCA+AGG 0.462038 6_2:+78725658 Msa0929910:CDS
GAGTGTAGGCAGATGGTTAG+TGG 0.473378 6_2:+78727624 Msa0929910:CDS
CTGATTGGTGATTCTGGAGT+TGG 0.474745 6_2:+78725686 Msa0929910:CDS
TTGAGGAAGAAGAAGCGAAA+GGG 0.477892 6_2:+78727725 Msa0929910:CDS
GTTGGAAACAAATGTGATCT+TGG 0.485814 6_2:+78727688 Msa0929910:CDS
TCGTATTATAGAGGAGCCTT+GGG 0.495142 6_2:+78727559 Msa0929910:CDS
ATCACAAGTTCGTATTATAG+AGG 0.496020 6_2:+78727550 Msa0929910:CDS
ACTCATCAAAGCTCAAATAT+GGG 0.497816 6_2:+78725814 Msa0929910:CDS
AAAGGGTTTGCAGAGGCAGA+AGG 0.511447 6_2:+78727742 Msa0929910:CDS
TACAATAAGTTCCAAAGAGA+AGG 0.512569 6_2:-78725562 None:intergenic
TGATTGGTGATTCTGGAGTT+GGG 0.520899 6_2:+78725687 Msa0929910:CDS
GGGACACTGCCGGCCAAGAA+AGG 0.528856 6_2:+78725834 Msa0929910:CDS
TTGTTCAAGGCAGTGCTGAT+TGG 0.534857 6_2:+78725671 Msa0929910:CDS
TGCAAAAGATGAATTCAGGT+TGG 0.536108 6_2:+78725733 Msa0929910:CDS
AGAGTTTGGTGGCGAAGAGA+TGG 0.552353 6_2:+78727654 Msa0929910:CDS
GGGAAGTTACCTTTCTTGGC+CGG 0.553345 6_2:-78725843 None:intergenic
ACACCAATTAATCTATCTAA+CGG 0.555332 6_2:+78727886 Msa0929910:CDS
ATACACTAGCAATGCTCCCA+AGG 0.558289 6_2:-78727575 None:intergenic
GTGGAGCTACGAGAGTTTGG+TGG 0.561282 6_2:+78727643 Msa0929910:CDS
TGGTGAATCAAGAGAAGTTG+AGG 0.568427 6_2:+78727708 Msa0929910:CDS
ATTTGCATACAGAAACATCA+AGG 0.574857 6_2:+78725781 Msa0929910:CDS
GTTGAGGAAGAAGAAGCGAA+AGG 0.577995 6_2:+78727724 Msa0929910:CDS
GCTCAAATATGGGACACTGC+CGG 0.581270 6_2:+78725824 Msa0929910:CDS
GTTCAGATTCTTCAAAGCAG+AGG 0.583572 6_2:-78727782 None:intergenic
TGTTGTTCATGATCGATGAG+AGG 0.584982 6_2:+78727958 Msa0929910:exon
CGATCAAGTTATGAGAGTGT+AGG 0.595817 6_2:+78727610 Msa0929910:CDS
GTTATGAGAGTGTAGGCAGA+TGG 0.605062 6_2:+78727617 Msa0929910:CDS
ACAAGGAGCCTGTTTAGTTG+CGG 0.617033 6_2:-78727938 None:intergenic
AAGGGTTTGCAGAGGCAGAA+GGG 0.631053 6_2:+78727743 Msa0929910:CDS
AGAAGCGAAAGGGTTTGCAG+AGG 0.645160 6_2:+78727735 Msa0929910:CDS
CTCATCGATCATGAACAACA+AGG 0.664580 6_2:-78727955 None:intergenic
TGCTAGTGTATGACATAACG+AGG 0.677719 6_2:+78727587 Msa0929910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTATTCATAACTTTA+TGG - chr6_2:78725943-78725962 None:intergenic 10.0%
!!! TTTGTTTTTTAACTTGTTAA+CGG + chr6_2:78726443-78726462 Msa0929910:intron 15.0%
!!! TAAGCTTTCTTTTTATAAAT+CGG + chr6_2:78726595-78726614 Msa0929910:intron 15.0%
!!! AATAGTGCAATTATTTTTAT+GGG - chr6_2:78726765-78726784 None:intergenic 15.0%
!!! AAATAGTGCAATTATTTTTA+TGG - chr6_2:78726766-78726785 None:intergenic 15.0%
!! TTACATTATTATTTACATGA+TGG - chr6_2:78726978-78726997 None:intergenic 15.0%
!! TTTCTAATCATATAAAACTT+AGG + chr6_2:78727165-78727184 Msa0929910:intron 15.0%
!! TGATTAATTAATTAATTGTG+TGG + chr6_2:78727508-78727527 Msa0929910:intron 15.0%
!!! CTAATTTTGTCAAATTCAAT+TGG - chr6_2:78726334-78726353 None:intergenic 20.0%
!! GAAAAGAAAACTAAAGATAA+AGG - chr6_2:78726817-78726836 None:intergenic 20.0%
!! CTACAAAAATATACTACTAT+AGG - chr6_2:78727254-78727273 None:intergenic 20.0%
!! ACTATAACAAATATATGGAT+GGG + chr6_2:78727476-78727495 Msa0929910:intron 20.0%
!!! TTTTGTTTTCCAATTCAAAG+TGG + chr6_2:78726060-78726079 Msa0929910:intron 25.0%
!!! TTTGTTTTCCAATTCAAAGT+GGG + chr6_2:78726061-78726080 Msa0929910:intron 25.0%
! AAGATAAGATATCATCATCA+CGG - chr6_2:78726284-78726303 None:intergenic 25.0%
! TTAAAAAACAAAACCACAGT+TGG - chr6_2:78726435-78726454 None:intergenic 25.0%
! GAATTGCTTAATCTCTTTAT+TGG + chr6_2:78726487-78726506 Msa0929910:intron 25.0%
! CTTAATCTCTTTATTGGAAT+TGG + chr6_2:78726493-78726512 Msa0929910:intron 25.0%
! AAGCTTAAAAGTAGAAAGTT+GGG - chr6_2:78726582-78726601 None:intergenic 25.0%
! AAAGCTTAAAAGTAGAAAGT+TGG - chr6_2:78726583-78726602 None:intergenic 25.0%
! TAAATGAATAGCGTAACTAT+TGG + chr6_2:78727006-78727025 Msa0929910:intron 25.0%
! AAATGAATAGCGTAACTATT+GGG + chr6_2:78727007-78727026 Msa0929910:intron 25.0%
! GATTAGAAAGATGAAATTGT+TGG - chr6_2:78727154-78727173 None:intergenic 25.0%
! TATAAAACTTAGGTATTCCA+TGG + chr6_2:78727175-78727194 Msa0929910:intron 25.0%
!! ATGAAATATTTGTTAGACCT+CGG + chr6_2:78727337-78727356 Msa0929910:intron 25.0%
! ATAAAACATATTGAGAGCAA+TGG - chr6_2:78727438-78727457 None:intergenic 25.0%
! GACTCACTATAACAAATATA+TGG + chr6_2:78727471-78727490 Msa0929910:intron 25.0%
! CACTATAACAAATATATGGA+TGG + chr6_2:78727475-78727494 Msa0929910:intron 25.0%
! CTATAACAAATATATGGATG+GGG + chr6_2:78727477-78727496 Msa0929910:intron 25.0%
! TATAACAAATATATGGATGG+GGG + chr6_2:78727478-78727497 Msa0929910:intron 25.0%
! ACACCAATTAATCTATCTAA+CGG + chr6_2:78727886-78727905 Msa0929910:CDS 25.0%
GAGCGAAGAAAGTAAAAATA+GGG - chr6_2:78725520-78725539 None:intergenic 30.0%
AGAGCGAAGAAAGTAAAAAT+AGG - chr6_2:78725521-78725540 None:intergenic 30.0%
TACAATAAGTTCCAAAGAGA+AGG - chr6_2:78725565-78725584 None:intergenic 30.0%
ATTTGCATACAGAAACATCA+AGG + chr6_2:78725781-78725800 Msa0929910:CDS 30.0%
AACTCATCAAAGCTCAAATA+TGG + chr6_2:78725813-78725832 Msa0929910:CDS 30.0%
ACTCATCAAAGCTCAAATAT+GGG + chr6_2:78725814-78725833 Msa0929910:CDS 30.0%
! AGAAAGACTGATTCTTTGAT+TGG - chr6_2:78725871-78725890 None:intergenic 30.0%
TTATGGATTTCGTTCATCAT+TGG - chr6_2:78725926-78725945 None:intergenic 30.0%
! TTGTTTTCCAATTCAAAGTG+GGG + chr6_2:78726062-78726081 Msa0929910:intron 30.0%
TAACTAGAATTCAATCAGAG+TGG + chr6_2:78726368-78726387 Msa0929910:intron 30.0%
AACTAGAATTCAATCAGAGT+GGG + chr6_2:78726369-78726388 Msa0929910:intron 30.0%
TTATGATCTTAGTGTCTTCA+GGG - chr6_2:78726396-78726415 None:intergenic 30.0%
ATTATGATCTTAGTGTCTTC+AGG - chr6_2:78726397-78726416 None:intergenic 30.0%
TCTCTTTATTGGAATTGGAA+TGG + chr6_2:78726498-78726517 Msa0929910:intron 30.0%
AGCTTAAAAGTAGAAAGTTG+GGG - chr6_2:78726581-78726600 None:intergenic 30.0%
!! TCTCTAGAATAAGTTGAACA+AGG - chr6_2:78727054-78727073 None:intergenic 30.0%
ATACCTCATACTTAGTCTAA+AGG + chr6_2:78727292-78727311 Msa0929910:intron 30.0%
ATTAATTAATTGTGTGGCAC+TGG + chr6_2:78727514-78727533 Msa0929910:intron 30.0%
ATCACAAGTTCGTATTATAG+AGG + chr6_2:78727550-78727569 Msa0929910:CDS 30.0%
CACCAATTAATCTATCTAAC+GGG + chr6_2:78727887-78727906 Msa0929910:CDS 30.0%
TTCCCGTTAGATAGATTAAT+TGG - chr6_2:78727892-78727911 None:intergenic 30.0%
TTCCAAAGAGAAGGTTATTC+TGG - chr6_2:78725556-78725575 None:intergenic 35.0%
AGAGTGTGATTACTTGTTCA+AGG + chr6_2:78725658-78725677 Msa0929910:CDS 35.0%
GGAAATCAAATCTGCTTTCA+AGG + chr6_2:78725708-78725727 Msa0929910:CDS 35.0%
GGTTTGCAAAAGATGAATTC+AGG + chr6_2:78725729-78725748 Msa0929910:CDS 35.0%
TGCAAAAGATGAATTCAGGT+TGG + chr6_2:78725733-78725752 Msa0929910:CDS 35.0%
TTGGATTCAAAGCCAACTAT+AGG + chr6_2:78725752-78725771 Msa0929910:CDS 35.0%
GCAAATTCAACTCCTATAGT+TGG - chr6_2:78725767-78725786 None:intergenic 35.0%
! GACTGATTCTTTGATTGGAA+GGG - chr6_2:78725866-78725885 None:intergenic 35.0%
! AGACTGATTCTTTGATTGGA+AGG - chr6_2:78725867-78725886 None:intergenic 35.0%
ATATTCATTCGCAAACCCAT+TGG - chr6_2:78726089-78726108 None:intergenic 35.0%
CACAGTTGGAATTCAATTCA+CGG - chr6_2:78726421-78726440 None:intergenic 35.0%
ACGGAATGTAATACTGCTTA+AGG + chr6_2:78726462-78726481 Msa0929910:intron 35.0%
CGGAATGTAATACTGCTTAA+GGG + chr6_2:78726463-78726482 Msa0929910:intron 35.0%
ATTGGAATTGGAATGGAAAG+TGG + chr6_2:78726505-78726524 Msa0929910:intron 35.0%
AAGTAGAAAGTTGGGGAAAA+AGG - chr6_2:78726574-78726593 None:intergenic 35.0%
! TTTCTCATGATGAAAGTGCA+AGG - chr6_2:78726839-78726858 None:intergenic 35.0%
ATGATGTGAGTTCAAAGAGT+AGG + chr6_2:78726900-78726919 Msa0929910:intron 35.0%
AAGAGCAGATAGACAATCAA+TGG + chr6_2:78727102-78727121 Msa0929910:intron 35.0%
ATAGACAATCAATGGATGTG+AGG + chr6_2:78727110-78727129 Msa0929910:intron 35.0%
AGTCCTTTAGACTAAGTATG+AGG - chr6_2:78727298-78727317 None:intergenic 35.0%
! AGATTTCAGTCCTTTTGATC+TGG - chr6_2:78727364-78727383 None:intergenic 35.0%
AAAGGACTGAAATCTCATGT+CGG + chr6_2:78727369-78727388 Msa0929910:intron 35.0%
ATTGAGAGCAATGGTTTGAT+GGG - chr6_2:78727429-78727448 None:intergenic 35.0%
TATTGAGAGCAATGGTTTGA+TGG - chr6_2:78727430-78727449 None:intergenic 35.0%
GAGATGGTAGTTATTCTAGT+TGG + chr6_2:78727670-78727689 Msa0929910:CDS 35.0%
GTTGGAAACAAATGTGATCT+TGG + chr6_2:78727688-78727707 Msa0929910:CDS 35.0%
!! AATTGGTGTCTCATCCATTT+TGG - chr6_2:78727875-78727894 None:intergenic 35.0%
GTCCAGAATAACCTTCTCTT+TGG + chr6_2:78725551-78725570 Msa0929910:five_prime_UTR 40.0%
!! TGATTGGTGATTCTGGAGTT+GGG + chr6_2:78725687-78725706 Msa0929910:CDS 40.0%
AATTCAAAGTGGGGTCCAAT+GGG + chr6_2:78726071-78726090 Msa0929910:intron 40.0%
CGTGAATTGAATTCCAACTG+TGG + chr6_2:78726419-78726438 Msa0929910:intron 40.0%
TTTAATCACTCCGAGTGATG+TGG + chr6_2:78726649-78726668 Msa0929910:intron 40.0%
CAAAGAGTAGGATTCACTCT+TGG + chr6_2:78726912-78726931 Msa0929910:intron 40.0%
TAGGTATTCCATGGAGTTAG+AGG + chr6_2:78727184-78727203 Msa0929910:intron 40.0%
! CTGAGTATATTGGTTATGGC+TGG - chr6_2:78727406-78727425 None:intergenic 40.0%
! TGGGCTGAGTATATTGGTTA+TGG - chr6_2:78727410-78727429 None:intergenic 40.0%
!! GTTTGATGGGCTGAGTATAT+TGG - chr6_2:78727416-78727435 None:intergenic 40.0%
TTCGTATTATAGAGGAGCCT+TGG + chr6_2:78727558-78727577 Msa0929910:CDS 40.0%
TCGTATTATAGAGGAGCCTT+GGG + chr6_2:78727559-78727578 Msa0929910:CDS 40.0%
TGCTAGTGTATGACATAACG+AGG + chr6_2:78727587-78727606 Msa0929910:CDS 40.0%
! CGATCAAGTTATGAGAGTGT+AGG + chr6_2:78727610-78727629 Msa0929910:CDS 40.0%
TGGTGAATCAAGAGAAGTTG+AGG + chr6_2:78727708-78727727 Msa0929910:CDS 40.0%
TTGAGGAAGAAGAAGCGAAA+GGG + chr6_2:78727725-78727744 Msa0929910:CDS 40.0%
GTTCAGATTCTTCAAAGCAG+AGG - chr6_2:78727785-78727804 None:intergenic 40.0%
CATCACAAACCAGAGAAGTT+TGG + chr6_2:78727846-78727865 Msa0929910:CDS 40.0%
! TTTTGGCATCCAAACTTCTC+TGG - chr6_2:78727858-78727877 None:intergenic 40.0%
GAGAAGTTTGGATGCCAAAA+TGG + chr6_2:78727858-78727877 Msa0929910:CDS 40.0%
TGAAGTTACCGCAACTAAAC+AGG + chr6_2:78727930-78727949 Msa0929910:CDS 40.0%
CTCATCGATCATGAACAACA+AGG - chr6_2:78727958-78727977 None:intergenic 40.0%
TGTTGTTCATGATCGATGAG+AGG + chr6_2:78727958-78727977 Msa0929910:exon 40.0%
! TCATGATCGATGAGAGGTTT+TGG + chr6_2:78727964-78727983 Msa0929910:exon 40.0%
!! TTGTTCAAGGCAGTGCTGAT+TGG + chr6_2:78725671-78725690 Msa0929910:CDS 45.0%
!! CTGATTGGTGATTCTGGAGT+TGG + chr6_2:78725686-78725705 Msa0929910:CDS 45.0%
GGAAGGGAAGTTACCTTTCT+TGG - chr6_2:78725850-78725869 None:intergenic 45.0%
CATTGGACCCCACTTTGAAT+TGG - chr6_2:78726072-78726091 None:intergenic 45.0%
CAATTCAAAGTGGGGTCCAA+TGG + chr6_2:78726070-78726089 Msa0929910:intron 45.0%
CACAGCAAAGGAGGAAATCA+CGG - chr6_2:78726538-78726557 None:intergenic 45.0%
! GTGATTTCCTCCTTTGCTGT+GGG + chr6_2:78726537-78726556 Msa0929910:intron 45.0%
AAGGGTTCATCCACATCACT+CGG - chr6_2:78726662-78726681 None:intergenic 45.0%
AGCGTAACTATTGGGAGAAG+CGG + chr6_2:78727015-78727034 Msa0929910:intron 45.0%
AGTTGAACAAGGCCATGTCA+TGG - chr6_2:78727043-78727062 None:intergenic 45.0%
GACAATCAATGGATGTGAGG+TGG + chr6_2:78727113-78727132 Msa0929910:intron 45.0%
! ATGTGAGGTGGTGAAGGTTT+TGG + chr6_2:78727125-78727144 Msa0929910:intron 45.0%
!! ATTAATTGTGTGGCACTGGC+AGG + chr6_2:78727518-78727537 Msa0929910:intron 45.0%
ATACACTAGCAATGCTCCCA+AGG - chr6_2:78727578-78727597 None:intergenic 45.0%
! GTTATGAGAGTGTAGGCAGA+TGG + chr6_2:78727617-78727636 Msa0929910:CDS 45.0%
TTAGTGGAGCTACGAGAGTT+TGG + chr6_2:78727640-78727659 Msa0929910:CDS 45.0%
GTTGAGGAAGAAGAAGCGAA+AGG + chr6_2:78727724-78727743 Msa0929910:CDS 45.0%
! GGCAGAAGGGTTGTGTTTTA+TGG + chr6_2:78727756-78727775 Msa0929910:CDS 45.0%
ACAAGGAGCCTGTTTAGTTG+CGG - chr6_2:78727941-78727960 None:intergenic 45.0%
!! TGATCGATGAGAGGTTTTGG+TGG + chr6_2:78727967-78727986 Msa0929910:exon 45.0%
!! GATCGATGAGAGGTTTTGGT+GGG + chr6_2:78727968-78727987 Msa0929910:exon 45.0%
!! ATCGATGAGAGGTTTTGGTG+GGG + chr6_2:78727969-78727988 Msa0929910:exon 45.0%
!! GATATATATATATATATTAA+TGG + chr6_2:78725610-78725629 Msa0929910:exon 5.0%
!! AATATAAAATTATCTTAAAA+GGG - chr6_2:78726680-78726699 None:intergenic 5.0%
!! AAATATAAAATTATCTTAAA+AGG - chr6_2:78726681-78726700 None:intergenic 5.0%
!! GCAGTGCTGATTGGTGATTC+TGG + chr6_2:78725680-78725699 Msa0929910:CDS 50.0%
GCTCAAATATGGGACACTGC+CGG + chr6_2:78725824-78725843 Msa0929910:CDS 50.0%
GGGAAGTTACCTTTCTTGGC+CGG - chr6_2:78725846-78725865 None:intergenic 50.0%
CGTGATTTCCTCCTTTGCTG+TGG + chr6_2:78726536-78726555 Msa0929910:intron 50.0%
!! ACAAGAAGCACCCACAGCAA+AGG - chr6_2:78726550-78726569 None:intergenic 50.0%
GCGTAACTATTGGGAGAAGC+GGG + chr6_2:78727016-78727035 Msa0929910:intron 50.0%
CAATGGATGTGAGGTGGTGA+AGG + chr6_2:78727119-78727138 Msa0929910:intron 50.0%
GAGACACACCTCTAACTCCA+TGG - chr6_2:78727195-78727214 None:intergenic 50.0%
AGACCTCGGACCAGATCAAA+AGG + chr6_2:78727351-78727370 Msa0929910:intron 50.0%
! AGTCCTTTTGATCTGGTCCG+AGG - chr6_2:78727357-78727376 None:intergenic 50.0%
GAGTGTAGGCAGATGGTTAG+TGG + chr6_2:78727624-78727643 Msa0929910:CDS 50.0%
!! AGAGTTTGGTGGCGAAGAGA+TGG + chr6_2:78727654-78727673 Msa0929910:CDS 50.0%
AGAAGCGAAAGGGTTTGCAG+AGG + chr6_2:78727735-78727754 Msa0929910:CDS 50.0%
AAAGGGTTTGCAGAGGCAGA+AGG + chr6_2:78727742-78727761 Msa0929910:CDS 50.0%
AAGGGTTTGCAGAGGCAGAA+GGG + chr6_2:78727743-78727762 Msa0929910:CDS 50.0%
!! AGAAGCACCCACAGCAAAGG+AGG - chr6_2:78726547-78726566 None:intergenic 55.0%
GTGGAGCTACGAGAGTTTGG+TGG + chr6_2:78727643-78727662 Msa0929910:CDS 55.0%
GGAGAAGCGGGTCCATGACA+TGG + chr6_2:78727028-78727047 Msa0929910:intron 60.0%
GGGACACTGCCGGCCAAGAA+AGG + chr6_2:78725834-78725853 Msa0929910:CDS 65.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 78725510 78728047 78725510 ID=Msa0929910;Name=Msa0929910
chr6_2 mRNA 78725510 78728047 78725510 ID=Msa0929910-mRNA-1;Parent=Msa0929910;Name=Msa0929910-mRNA-1;_AED=0.08;_eAED=0.08;_QI=119|1|1|1|1|1|2|78|218
chr6_2 exon 78725510 78725855 78725510 ID=Msa0929910-mRNA-1:exon:13318;Parent=Msa0929910-mRNA-1
chr6_2 exon 78727540 78728047 78727540 ID=Msa0929910-mRNA-1:exon:13319;Parent=Msa0929910-mRNA-1
chr6_2 five_prime_UTR 78725510 78725628 78725510 ID=Msa0929910-mRNA-1:five_prime_utr;Parent=Msa0929910-mRNA-1
chr6_2 CDS 78725629 78725855 78725629 ID=Msa0929910-mRNA-1:cds;Parent=Msa0929910-mRNA-1
chr6_2 CDS 78727540 78727969 78727540 ID=Msa0929910-mRNA-1:cds;Parent=Msa0929910-mRNA-1
chr6_2 three_prime_UTR 78727970 78728047 78727970 ID=Msa0929910-mRNA-1:three_prime_utr;Parent=Msa0929910-mRNA-1
Gene Sequence

>Msa0929910

ATGGATCATGATGCAATTATATTTGATGAAGAGTGTGATTACTTGTTCAAGGCAGTGCTGATTGGTGATTCTGGAGTTGGGAAATCAAATCTGCTTTCAAGGTTTGCAAAAGATGAATTCAGGTTGGATTCAAAGCCAACTATAGGAGTTGAATTTGCATACAGAAACATCAAGGTCAGAGACAAACTCATCAAAGCTCAAATATGGGACACTGCCGGCCAAGAAAGGTTTAGAGCTATCACAAGTTCGTATTATAGAGGAGCCTTGGGAGCATTGCTAGTGTATGACATAACGAGGCGATCAAGTTATGAGAGTGTAGGCAGATGGTTAGTGGAGCTACGAGAGTTTGGTGGCGAAGAGATGGTAGTTATTCTAGTTGGAAACAAATGTGATCTTGGTGAATCAAGAGAAGTTGAGGAAGAAGAAGCGAAAGGGTTTGCAGAGGCAGAAGGGTTGTGTTTTATGGAAACCTCTGCTTTGAAGAATCTGAACGTTGAGCAAGTGTTCTTACAAATGATCACAACTATTTTTGACATCACAAACCAGAGAAGTTTGGATGCCAAAATGGATGAGACACCAATTAATCTATCTAACGGGAAAGAGATTCATATAGCTGATGAAGTTACCGCAACTAAACAGGCTCCTTGTTGTTCATGA
Protein sequence

>Msa0929910

MDHDAIIFDEECDYLFKAVLIGDSGVGKSNLLSRFAKDEFRLDSKPTIGVEFAYRNIKVRDKLIKAQIWDTAGQERFRAITSSYYRGALGALLVYDITRRSSYESVGRWLVELREFGGEEMVVILVGNKCDLGESREVEEEEAKGFAEAEGLCFMETSALKNLNVEQVFLQMITTIFDITNQRSLDAKMDETPINLSNGKEIHIADEVTATKQAPCCS*