AlfalfaGEDB | Msa0930710

M. sativa cultivar zhongmu-4 / Msa0930710

KEGG: K09419
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00447.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0930710	XP_024629287.1	97.574	371	5	1	1	367	1	371	0.0	747

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0930710	sp\|Q9S7U5\|HSFA8_ARATH	43.697	357	160	6	16	356	17	348	1.06e-88	275

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0930710	TF	HSF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001870	Msa0930710	0.874088	8.871937e-68	-8.615850e-47
Msa0576750	Msa0930710	0.877025	8.743507e-69	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0930710	MtrunA17_Chr8g0392001	97.574	371	5	1	1	367	1	371	0.0	747
Msa0930710	MtrunA17_Chr5g0410881	45.756	271	129	6	18	272	32	300	5.67e-67	219
Msa0930710	MtrunA17_Chr4g0039891	42.910	268	145	3	18	278	12	278	2.15e-66	218
Msa0930710	MtrunA17_Chr1g0204741	49.495	198	90	4	18	215	40	227	1.08e-55	185
Msa0930710	MtrunA17_Chr7g0260331	47.938	194	97	2	18	211	49	238	2.39e-53	180
Msa0930710	MtrunA17_Chr8g0379701	45.274	201	99	3	9	207	3	194	5.24e-50	172
Msa0930710	MtrunA17_Chr3g0135831	45.361	194	99	2	18	211	46	232	8.15e-50	171
Msa0930710	MtrunA17_Chr6g0467041	46.377	207	96	7	4	210	10	201	1.97e-48	166
Msa0930710	MtrunA17_Chr1g0185131	45.161	186	84	3	18	203	32	199	7.19e-47	162
Msa0930710	MtrunA17_Chr6g0485571	46.193	197	95	4	16	212	9	194	2.46e-45	159

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0930710	AT1G67970.1	43.697	357	160	6	16	356	17	348	1.08e-89	275
Msa0930710	AT1G32330.1	46.000	250	123	4	13	251	32	280	6.68e-63	209
Msa0930710	AT1G32330.2	46.000	250	123	4	13	251	32	280	6.72e-63	209
Msa0930710	AT5G16820.1	46.694	242	119	4	15	248	24	263	5.47e-60	201
Msa0930710	AT5G16820.2	46.694	242	119	4	15	248	24	263	5.47e-60	201
Msa0930710	AT4G17750.1	46.341	246	112	5	18	244	52	296	3.38e-59	199
Msa0930710	AT3G22830.1	49.261	203	101	2	18	219	61	262	6.34e-56	188
Msa0930710	AT2G26150.1	49.735	189	88	2	18	206	44	225	7.75e-56	186
Msa0930710	AT2G26150.4	49.735	189	88	2	18	206	44	225	7.75e-56	186
Msa0930710	AT3G02990.1	48.848	217	107	3	4	219	9	222	1.27e-55	189

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Find 125 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGCGTGGAGTCGAGGG+AGG	0.624843	6_2:-79775547	Msa0930710:five_prime_UTR
AAACAACTGCTGGAGGAAGA+TGG	0.498422	6_2:+79773301	None:intergenic
AAACGCGTGGAGTCGAGGGA+GGG	0.644599	6_2:-79775546	Msa0930710:five_prime_UTR
AACCCATCTGTAGAAACCTC+AGG	0.474952	6_2:+79773783	None:intergenic
AACCTGAGGTTTCTACAGAT+GGG	0.578276	6_2:-79773785	Msa0930710:CDS
AACGGAGAATGATTATCTAA+TGG	0.359885	6_2:+79773702	None:intergenic
AACTGTTAGTGACATGGAGA+TGG	0.556871	6_2:-79773577	Msa0930710:CDS
AAGAAACTGGTCTGTCATCT+TGG	0.448044	6_2:+79773898	None:intergenic
AAGCAGTAAATCATGGCCTT+TGG	0.439818	6_2:-79774178	Msa0930710:CDS
AATAATCAATTAACCTAAAG+AGG	0.447384	6_2:+79775370	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTACAAACAAAACAAAGACT+TGG	+	chr6_2:79772964-79772983	None:intergenic	30.0%
AAACTGATATAGGGTTAATG+AGG	+	chr6_2:79773433-79773452	None:intergenic	30.0%
TGTAGTAAAGGTTTCTCAAA+AGG	+	chr6_2:79773953-79773972	None:intergenic	30.0%
ACACTTTAAACAAATCGAGA+CGG	+	chr6_2:79774047-79774066	None:intergenic	30.0%
TGATGACTGTTGAATTTATC+AGG	-	chr6_2:79774088-79774107	Msa0930710:CDS	30.0%
GATGACTGTTGAATTTATCA+GGG	-	chr6_2:79774089-79774108	Msa0930710:CDS	30.0%
GCAAATGAAAACTTCATTAG+GGG	-	chr6_2:79774146-79774165	Msa0930710:CDS	30.0%
TTCACCCTAAGGAAAATAAT+TGG	-	chr6_2:79774495-79774514	Msa0930710:intron	30.0%
ACGGAGAATGATTATCTAAT+GGG	+	chr6_2:79774754-79774773	None:intergenic	30.0%
AACGGAGAATGATTATCTAA+TGG	+	chr6_2:79774755-79774774	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	79772903	79775573	79772903	ID=Msa0930710;Name=Msa0930710
chr6_2	mRNA	79772903	79775573	79772903	ID=Msa0930710-mRNA-1;Parent=Msa0930710;Name=Msa0930710-mRNA-1;_AED=0.03;_eAED=0.03;_QI=214\|1\|1\|1\|0.5\|0.33\|3\|488\|367
chr6_2	exon	79772903	79773174	79772903	ID=Msa0930710-mRNA-1:exon:13824;Parent=Msa0930710-mRNA-1
chr6_2	exon	79773281	79774366	79773281	ID=Msa0930710-mRNA-1:exon:13823;Parent=Msa0930710-mRNA-1
chr6_2	exon	79775126	79775573	79775126	ID=Msa0930710-mRNA-1:exon:13822;Parent=Msa0930710-mRNA-1
chr6_2	five_prime_UTR	79775360	79775573	79775360	ID=Msa0930710-mRNA-1:five_prime_utr;Parent=Msa0930710-mRNA-1
chr6_2	CDS	79775126	79775359	79775126	ID=Msa0930710-mRNA-1:cds;Parent=Msa0930710-mRNA-1
chr6_2	CDS	79773497	79774366	79773497	ID=Msa0930710-mRNA-1:cds;Parent=Msa0930710-mRNA-1
chr6_2	three_prime_UTR	79773281	79773496	79773281	ID=Msa0930710-mRNA-1:three_prime_utr;Parent=Msa0930710-mRNA-1
chr6_2	three_prime_UTR	79772903	79773174	79772903	ID=Msa0930710-mRNA-1:three_prime_utr;Parent=Msa0930710-mRNA-1

Gene Sequence

>Msa0930710

ATGGTGAAAGCAGCAACAGCGAGCGAGAAGGGTGGTTCTGGTTCGGTGGCACCTTTCTTGAATAAATGTTACGATATGGTTGAAGATCCTTCAACCGATTCCGTCATCTCTTGGTCCGCTGATGGTAACAATAGTTTCATCATCTCCAACGCTGATCAATTCTCACTCACTCTTCTCCCCAATTATTTTAAACACAATAATTTCTCCAGCTTCGTCAGACAACTCAACATCTATGGTTTCAGGAAAATTGATGCAGATCACTGGGAATTTGCAAATGAAAACTTCATTAGGGGTCAAAAGCATTTGCTGAAGAATATACGTAGAAGGAAACATCCACATGTTGCTGCAGATCAGCAAAAACCATTGCCGCCAAAAGACAATCGCGATGAACCATCACAAGAAGCAGTAAATCATGGCCTTTGGAGAGAAGTTGAGAATCTAAAATCGGATAGAAAAACCCTCACGCAGGAGTTGGTCAAACATAAGCAGCACCTTGAATCTTCTGAGAGTAAGCTGCTTCTACTGAGTGATCGCCTTGAAGGAATGGAGAAGCATCAGCAGCAGATGCTCTCATTCTTGGTCATGGTCGTGCAATGCCCTGGATTCTTGGTTCAGCTGCTTCACCCTAAGGAAAATAATTGGCGTTTCTCTGAAGCAGGGAATATGTGGGACCAAGGTAACCAAGATGACAGACCAGTTTCTTCTGATGGGATGATTATAAAGTATACACCACCTGTTGCTGAAAAACTAAAACCTGTTGTTCCACGATCTCCTGCCTTTAATAAGCAACCAGAACCTGAGGTTTCTACAGATGGGTTAAAAGACTTGTGCATCAGTTCTGAGTTCTTGAAATTGCTTCTAGATGAGAAATTGTGCCCATTAGATAATCATTCTCCGTTTCTTGTACCTGATTTACCGGATGACGGTTCGTGGGAACAACTATTTTTAGGCAGTCCTATCCTAAAAAATGTTGAAGATACTGATCGTGAAAACGAGGAGCAAACTGTTAGTGACATGGAGATGGATCACATCATATCTGAAGCTCTAAATGAAACATCTGATTTTGAAGCGATGATTATGGAAATGGAGAACACTCACGCATGA

Protein sequence

>Msa0930710

MVKAATASEKGGSGSVAPFLNKCYDMVEDPSTDSVISWSADGNNSFIISNADQFSLTLLPNYFKHNNFSSFVRQLNIYGFRKIDADHWEFANENFIRGQKHLLKNIRRRKHPHVAADQQKPLPPKDNRDEPSQEAVNHGLWREVENLKSDRKTLTQELVKHKQHLESSESKLLLLSDRLEGMEKHQQQMLSFLVMVVQCPGFLVQLLHPKENNWRFSEAGNMWDQGNQDDRPVSSDGMIIKYTPPVAEKLKPVVPRSPAFNKQPEPEVSTDGLKDLCISSEFLKLLLDEKLCPLDNHSPFLVPDLPDDGSWEQLFLGSPILKNVEDTDRENEEQTVSDMEMDHIISEALNETSDFEAMIMEMENTHA*