AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0935140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0935140 MtrunA17_Chr3g0111971 87.379 103 11 1 36 138 27 127 1.90e-54 185
Msa0935140 MtrunA17_Chr3g0111971 82.178 101 16 1 124 222 186 286 1.87e-38 141
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0935140 AT3G12380.1 64.844 128 42 2 11 138 3 127 3.45e-48 168
Msa0935140 AT3G12380.1 66.990 103 28 2 124 222 186 286 3.53e-40 146
Msa0935140 AT3G12380.2 61.940 134 42 3 11 138 3 133 7.23e-46 162
Msa0935140 AT3G12380.2 66.990 103 28 2 124 222 192 292 3.65e-40 146

Find 94 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTACCGGTCATATTTAT+TGG 0.189709 6_3:-5596761 Msa0935140:three_prime_UTR
CGACAGCTTCCTTTATAAAT+AGG 0.192795 6_3:+5598000 None:intergenic
CAAAATTAAGCATCCTTAAA+TGG 0.211174 6_3:+5596602 None:intergenic
CCAAGGGAGCTGCCACATTT+TGG 0.253326 6_3:+5597618 None:intergenic
TACTTTGATTGTACTCGTTC+TGG 0.294611 6_3:-5599951 Msa0935140:CDS
GCGCTTCCTATTTCCGTATT+GGG 0.301969 6_3:-5600394 Msa0935140:intron
CGTGTCGGGGCTCACCGTGC+TGG 0.313800 6_3:-5600422 Msa0935140:CDS
GGCGCTTCCTATTTCCGTAT+TGG 0.315333 6_3:-5600395 Msa0935140:CDS
GGAAAATGAATTACGTGGTT+TGG 0.322105 6_3:-5597462 Msa0935140:three_prime_UTR
AAAGTACCCAATACGGAAAT+AGG 0.324181 6_3:+5600388 None:intergenic
GAAAAGAAAGGCAGCAATAA+GGG 0.331676 6_3:-5597549 Msa0935140:exon
CGAATGGGTGCAACAGGATC+AGG 0.331988 6_3:-5599205 Msa0935140:CDS
AGAAAAGAAAGGCAGCAATA+AGG 0.339186 6_3:-5597550 Msa0935140:exon
CCAAAATGTGGCAGCTCCCT+TGG 0.342410 6_3:-5597618 Msa0935140:CDS
GGGGCTCACCGTGCTGGGGC+TGG 0.355011 6_3:-5600416 Msa0935140:CDS
GACGGGGAACCTATTTATAA+AGG 0.363787 6_3:-5598009 Msa0935140:CDS
GGAAGCTGTCGCACTAATAT+TGG 0.366748 6_3:-5597988 Msa0935140:CDS
GAAGTGCTATGAAGAGGAAT+TGG 0.378574 6_3:-5596982 Msa0935140:three_prime_UTR
TAGTTTAGTAAATGAATTAC+CGG 0.380279 6_3:-5596774 Msa0935140:three_prime_UTR
TCAGAAGGAGGCTCCTCAAC+TGG 0.380356 6_3:+5597586 None:intergenic
CAGTCCTTGCGGAAACAAAA+AGG 0.381377 6_3:-5597326 Msa0935140:three_prime_UTR
ACAACCCACCCGATCGTGTC+GGG 0.384348 6_3:-5600436 Msa0935140:CDS
ATAACAATCTTTCCATCTTC+AGG 0.387111 6_3:+5597786 None:intergenic
TTTGATCGAATGGGTGCAAC+AGG 0.394008 6_3:-5599211 Msa0935140:CDS
TTTGATAGCTTGTTGAAAAG+AGG 0.403014 6_3:-5597026 Msa0935140:intron
AGGTCAAAGGTTGCGAGAAA+TGG 0.404209 6_3:-5597519 Msa0935140:three_prime_UTR
GGAGAAACTGTTACCATTGT+TGG 0.413207 6_3:-5599999 Msa0935140:CDS
TCTGCCGTCAGCATGTCATC+AGG 0.414265 6_3:+5597239 None:intergenic
AGAGAGAGAGATTTAGCAGT+TGG 0.416915 6_3:-5596816 Msa0935140:three_prime_UTR
TAAATCGGTTTGTACTTTGT+AGG 0.443447 6_3:+5600495 None:intergenic
TTCTCAGTAGACGCAGCGTC+AGG 0.462710 6_3:+5597281 None:intergenic
CTTGCAATCTCTTCCTCAGA+AGG 0.464435 6_3:+5597571 None:intergenic
GGCTAGATTTACATGGGAAA+AGG 0.468160 6_3:-5597816 Msa0935140:CDS
AAATCGGTTTGTACTTTGTA+GGG 0.470081 6_3:+5600496 None:intergenic
CAAAATGTGGCAGCTCCCTT+GGG 0.477303 6_3:-5597617 Msa0935140:CDS
CGGTGAGCCCCGACACGATC+GGG 0.487843 6_3:+5600428 None:intergenic
TCAAAGTACTTCAACAATGC+TGG 0.488489 6_3:+5599966 None:intergenic
TTTCATACTCACGTATGATA+AGG 0.501112 6_3:+5597916 None:intergenic
ATAGCCAGTCTCTGCTAAGA+AGG 0.501279 6_3:+5597391 None:intergenic
TCAACTGGAGGTGGCACCCA+AGG 0.504173 6_3:+5597601 None:intergenic
GAAAGATTGTTATGTTGCAC+AGG 0.505276 6_3:-5597776 Msa0935140:CDS
GCTATGAAGAGGAATTGGTA+AGG 0.508107 6_3:-5596977 Msa0935140:three_prime_UTR
AAGAGGAATTGGTAAGGGAG+AGG 0.512761 6_3:-5596971 Msa0935140:three_prime_UTR
TTGGGTGCCACCTCCAGTTG+AGG 0.515845 6_3:-5597599 Msa0935140:CDS
CGATCCTTCTTAGCAGAGAC+TGG 0.517740 6_3:-5597395 Msa0935140:three_prime_UTR
AATACAGGGCTAGATTTACA+TGG 0.523587 6_3:-5597823 Msa0935140:intron
ACTTCAACAATGCTGGATCA+TGG 0.526588 6_3:+5599973 None:intergenic
AATTGAAGCATGGAACATAC+AGG 0.529482 6_3:+5596922 None:intergenic
CAGAGACTGGCTATGTCTCT+AGG 0.531810 6_3:-5597382 Msa0935140:three_prime_UTR
AAAAGAGAAGTGCTATGAAG+AGG 0.534586 6_3:-5596988 Msa0935140:three_prime_UTR
GACTGGCTATGTCTCTAGGC+AGG 0.538143 6_3:-5597378 Msa0935140:three_prime_UTR
AAAGGTTGAAGACCTGAAGA+TGG 0.539338 6_3:-5597798 Msa0935140:CDS
AGGACTGCACAACTTTACTA+CGG 0.540370 6_3:+5597342 None:intergenic
GGAGGCTCCTCAACTGGAGG+TGG 0.543167 6_3:+5597592 None:intergenic
AGCTGTCGCACTAATATTGG+TGG 0.543175 6_3:-5597985 Msa0935140:CDS
TGACATGCTGACGGCAGAGC+AGG 0.546133 6_3:-5597234 Msa0935140:intron
AAGAGATTGCAAGAAAAGAA+AGG 0.547623 6_3:-5597561 Msa0935140:CDS
ACGGTGAGCCCCGACACGAT+CGG 0.558422 6_3:+5600427 None:intergenic
CTTGTTCCAATAAATATGAC+CGG 0.563798 6_3:+5596755 None:intergenic
TAGGTTGGAGGATATAATTG+TGG 0.569810 6_3:+5596679 None:intergenic
CTATGAAGAGGAATTGGTAA+GGG 0.573363 6_3:-5596976 Msa0935140:three_prime_UTR
TGAGCCCCGACACGATCGGG+TGG 0.575680 6_3:+5600431 None:intergenic
CAACTGGAGGTGGCACCCAA+GGG 0.576342 6_3:+5597602 None:intergenic
GTTTGAGAAAGTACCCAATA+CGG 0.582666 6_3:+5600381 None:intergenic
AGTTGAGGAGCCTCCTTCTG+AGG 0.582751 6_3:-5597584 Msa0935140:CDS
GAATGGGTGCAACAGGATCA+GGG 0.585195 6_3:-5599204 Msa0935140:CDS
AACAACCCACCCGATCGTGT+CGG 0.591970 6_3:-5600437 Msa0935140:CDS
TGTCGGGGCTCACCGTGCTG+GGG 0.593810 6_3:-5600420 Msa0935140:CDS
GGTCTTGCAGAAAGGAGCTA+AGG 0.595543 6_3:-5597656 Msa0935140:intron
AGCTTGAATAAATTGAAGCA+TGG 0.599376 6_3:+5596912 None:intergenic
TAAGGGAGAAACAAGGTCAA+AGG 0.600031 6_3:-5597532 Msa0935140:three_prime_UTR
GGACTGCACAACTTTACTAC+GGG 0.602389 6_3:+5597343 None:intergenic
CTGCCGTCAGCATGTCATCA+GGG 0.602597 6_3:+5597240 None:intergenic
CAACCCACCCGATCGTGTCG+GGG 0.604755 6_3:-5600435 Msa0935140:CDS
AAAGGAGCTAAGGAGGCCAA+AGG 0.605406 6_3:-5597646 Msa0935140:CDS
TGGATCATGGTCACCAACAA+TGG 0.605508 6_3:+5599986 None:intergenic
CTTGCAGAAAGGAGCTAAGG+AGG 0.614499 6_3:-5597653 Msa0935140:intron
GCAATCTCTTCCTCAGAAGG+AGG 0.622040 6_3:+5597574 None:intergenic
AGGAAGCGCCAGCCCCAGCA+CGG 0.622653 6_3:+5600408 None:intergenic
CGGGCGCGACATAAAGCCAC+AGG 0.623244 6_3:-5600173 Msa0935140:intron
GGAGAGGAAACAACAGCTAG+AGG 0.635531 6_3:-5596955 Msa0935140:three_prime_UTR
GAGGAAACAACAGCTAGAGG+AGG 0.641074 6_3:-5596952 Msa0935140:three_prime_UTR
GAAGGAGGCTCCTCAACTGG+AGG 0.645561 6_3:+5597589 None:intergenic
GAGAAATGGCTGAGGCGAAG+AGG 0.646132 6_3:-5597505 Msa0935140:three_prime_UTR
GCTGAAAATAATCAGTCACA+TGG 0.648180 6_3:+5597959 None:intergenic
AAGGTTGCGAGAAATGGCTG+AGG 0.651418 6_3:-5597513 Msa0935140:three_prime_UTR
GAGCCCCGACACGATCGGGT+GGG 0.652167 6_3:+5600432 None:intergenic
GTAAAGTTGTGCAGTCCTTG+CGG 0.652419 6_3:-5597337 Msa0935140:three_prime_UTR
AATGGGTGCAACAGGATCAG+GGG 0.654606 6_3:-5599203 Msa0935140:intron
ATACAGGGCTAGATTTACAT+GGG 0.656838 6_3:-5597822 Msa0935140:intron
TCGCTTTCAAATGCTGATCG+TGG 0.660583 6_3:+5599927 None:intergenic
CATCCCTGATGACATGCTGA+CGG 0.663450 6_3:-5597243 Msa0935140:three_prime_UTR
GCAGCAATAAGGGAGAAACA+AGG 0.705579 6_3:-5597539 Msa0935140:exon
GAGCTGGAAAATGAATTACG+TGG 0.728785 6_3:-5597467 Msa0935140:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATAAATTTTTAAATAAAT+AGG - chr6_3:5598945-5598964 Msa0935140:intron 0.0%
!! AAAAACTAAACAATAAAAAA+TGG - chr6_3:5598913-5598932 Msa0935140:intron 10.0%
!!! ATTTTTTTGAGATGAAATTA+GGG + chr6_3:5596760-5596779 None:intergenic 15.0%
!!! AATTTTTTTGAGATGAAATT+AGG + chr6_3:5596761-5596780 None:intergenic 15.0%
!!! TTTTTATTTGAATTGTTGTT+GGG - chr6_3:5597051-5597070 Msa0935140:intron 15.0%
!! AATATGAATAATTTCTAACT+AGG - chr6_3:5597466-5597485 Msa0935140:three_prime_UTR 15.0%
!! ATATGAATAATTTCTAACTA+GGG - chr6_3:5597467-5597486 Msa0935140:three_prime_UTR 15.0%
!! CAATATTCAAAGAAATTATT+TGG + chr6_3:5600024-5600043 None:intergenic 15.0%
!!! TTTTGTTGACAAAGTTAAAT+CGG + chr6_3:5596639-5596658 None:intergenic 20.0%
!!! TTTAACTTTGTCAACAAAAA+AGG - chr6_3:5596640-5596659 Msa0935140:three_prime_UTR 20.0%
!!! AATTTTGAATTTTCTCATCA+TGG - chr6_3:5596823-5596842 Msa0935140:three_prime_UTR 20.0%
!!! ATTTTGAATTTTCTCATCAT+GGG - chr6_3:5596824-5596843 Msa0935140:three_prime_UTR 20.0%
!!! TTTTTGGTGAATTTTTAGAT+GGG - chr6_3:5596876-5596895 Msa0935140:three_prime_UTR 20.0%
!!! GTTTTTATTTGAATTGTTGT+TGG - chr6_3:5597050-5597069 Msa0935140:intron 20.0%
!! TTGGGATATTGAATAATTTA+TGG - chr6_3:5597069-5597088 Msa0935140:intron 20.0%
!!! TGTTTTTCAGTTTGAAATAA+AGG - chr6_3:5597215-5597234 Msa0935140:intron 20.0%
!! GAAATTATTCATATTACTAG+TGG + chr6_3:5597461-5597480 None:intergenic 20.0%
!! TACTTGTTCATACTATAAAA+AGG + chr6_3:5597609-5597628 None:intergenic 20.0%
!! TTATAGTATGAACAAGTAAA+TGG - chr6_3:5597611-5597630 Msa0935140:CDS 20.0%
!! TCATTCATATATTTCATACA+AGG + chr6_3:5597649-5597668 None:intergenic 20.0%
!! TGAAATATATGAATGAATTG+AGG - chr6_3:5597653-5597672 Msa0935140:intron 20.0%
!! TGTACCATATTACATATTAT+GGG - chr6_3:5597769-5597788 Msa0935140:CDS 20.0%
!! TATTCCCATAATATGTAATA+TGG + chr6_3:5597776-5597795 None:intergenic 20.0%
!! TATTACATATTATGGGAATA+TGG - chr6_3:5597776-5597795 Msa0935140:CDS 20.0%
!! TCAGTAATATTAAACTCTAA+TGG - chr6_3:5598009-5598028 Msa0935140:CDS 20.0%
!! TAATATTAAACTCTAATGGT+AGG - chr6_3:5598013-5598032 Msa0935140:CDS 20.0%
!!! TTATATTTTCAGTTTGTTGA+CGG - chr6_3:5599089-5599108 Msa0935140:intron 20.0%
!! TCTTATTAAATTCTCAATAC+AGG - chr6_3:5599278-5599297 Msa0935140:intron 20.0%
!! CTTATTAAATTCTCAATACA+GGG - chr6_3:5599279-5599298 Msa0935140:intron 20.0%
!! TAGTTTAGTAAATGAATTAC+CGG - chr6_3:5600342-5600361 Msa0935140:intron 20.0%
! CATCTAAAAATTCACCAAAA+AGG + chr6_3:5596877-5596896 None:intergenic 25.0%
!!! CTTTTTGGTGAATTTTTAGA+TGG - chr6_3:5596875-5596894 Msa0935140:three_prime_UTR 25.0%
!! TTTTTAACATTATGTGACAC+AGG - chr6_3:5597096-5597115 Msa0935140:intron 25.0%
! TATACTCACATAATTGTAAC+AGG + chr6_3:5597255-5597274 None:intergenic 25.0%
! TGTTACAATTATGTGAGTAT+AGG - chr6_3:5597254-5597273 Msa0935140:three_prime_UTR 25.0%
!!! ATAGGAGGAATAGTTTTTTT+AGG - chr6_3:5597272-5597291 Msa0935140:three_prime_UTR 25.0%
! GATCATTAACTAAAAAACAC+AGG + chr6_3:5597422-5597441 None:intergenic 25.0%
! TAATTTCTAACTAGGGAAAT+AGG - chr6_3:5597474-5597493 Msa0935140:three_prime_UTR 25.0%
! AATATGTAAATCCTAAGTAC+AGG + chr6_3:5597535-5597554 None:intergenic 25.0%
!!! CATTTTAGTGTGATAGTTTT+AGG + chr6_3:5597558-5597577 None:intergenic 25.0%
! TAAGTTCATAACAACTAGTT+AGG - chr6_3:5597742-5597761 Msa0935140:intron 25.0%
! AAGTTCATAACAACTAGTTA+GGG - chr6_3:5597743-5597762 Msa0935140:intron 25.0%
! GTGTACCATATTACATATTA+TGG - chr6_3:5597768-5597787 Msa0935140:CDS 25.0%
! ATATGGTCATTAATGTACAT+TGG - chr6_3:5597793-5597812 Msa0935140:CDS 25.0%
!!! CATTAATGTACATTGGTTTA+AGG - chr6_3:5597800-5597819 Msa0935140:CDS 25.0%
! ATAAAAAACCACTTTACTCA+TGG + chr6_3:5597944-5597963 None:intergenic 25.0%
! ATGATATAAGCTGCAAATTA+TGG + chr6_3:5598056-5598075 None:intergenic 25.0%
!!! GAACTTTTTTTTGAGACTTA+TGG - chr6_3:5598541-5598560 Msa0935140:intron 25.0%
!! AAAGTATTAGTACCTATAGA+TGG + chr6_3:5598571-5598590 None:intergenic 25.0%
!!! TAATACTTTTTAACCCACAA+TGG - chr6_3:5598582-5598601 Msa0935140:intron 25.0%
! AAACATTGTTAAAGAAACTG+TGG - chr6_3:5598872-5598891 Msa0935140:intron 25.0%
! AACATTGTTAAAGAAACTGT+GGG - chr6_3:5598873-5598892 Msa0935140:intron 25.0%
! TTATTAACACTTGACACATT+TGG + chr6_3:5599036-5599055 None:intergenic 25.0%
!!! TATATTTTCAGTTTGTTGAC+GGG - chr6_3:5599090-5599109 Msa0935140:intron 25.0%
! GTAAGAAATAGTACGTATTT+AGG + chr6_3:5599257-5599276 None:intergenic 25.0%
!! GAATTTCTTTTGAATCAACT+TGG - chr6_3:5599676-5599695 Msa0935140:intron 25.0%
! ATTAAGACTATAAGCACATA+TGG + chr6_3:5599999-5600018 None:intergenic 25.0%
! TCTTTGAATATTGTTTACTG+TGG - chr6_3:5600031-5600050 Msa0935140:intron 25.0%
!!! CTTGTTTTATTTTTGTAGGT+TGG + chr6_3:5600455-5600474 None:intergenic 25.0%
! CAACCTACAAAAATAAAACA+AGG - chr6_3:5600453-5600472 Msa0935140:CDS 25.0%
!!! AGTCCTTGTTTTATTTTTGT+AGG + chr6_3:5600459-5600478 None:intergenic 25.0%
!! AAATCGGTTTGTACTTTGTA+GGG + chr6_3:5596623-5596642 None:intergenic 30.0%
!! TAAATCGGTTTGTACTTTGT+AGG + chr6_3:5596624-5596643 None:intergenic 30.0%
!!! CTTTAAAGTTCGTTCCTTTT+TGG - chr6_3:5596860-5596879 Msa0935140:three_prime_UTR 30.0%
! TTTTTCGCAACATTGTTCAA+CGG - chr6_3:5596923-5596942 Msa0935140:three_prime_UTR 30.0%
TACAATTATGTGAGTATAGG+AGG - chr6_3:5597257-5597276 Msa0935140:three_prime_UTR 30.0%
!! GATATAGTGACCTATTTTGA+TGG - chr6_3:5597304-5597323 Msa0935140:three_prime_UTR 30.0%
GTTATGAACTTATGAGCATA+TGG + chr6_3:5597734-5597753 None:intergenic 30.0%
ATTATGGTGTTTATCCAAAC+AGG + chr6_3:5598040-5598059 None:intergenic 30.0%
GAACTTATGAAAACAGCTTA+TGG + chr6_3:5598234-5598253 None:intergenic 30.0%
AAACATGTATTGCAAAACCA+AGG + chr6_3:5598384-5598403 None:intergenic 30.0%
! TTTTACTTCGAGATTGAACT+GGG + chr6_3:5598516-5598535 None:intergenic 30.0%
! ATTTTACTTCGAGATTGAAC+TGG + chr6_3:5598517-5598536 None:intergenic 30.0%
AGTTCAATCTCGAAGTAAAA+TGG - chr6_3:5598516-5598535 Msa0935140:intron 30.0%
! TTTTCACTTAAGAATAGACC+TGG + chr6_3:5598692-5598711 None:intergenic 30.0%
TCTTGTGACATAGTTATTAC+TGG - chr6_3:5598751-5598770 Msa0935140:intron 30.0%
! TGACATAGTTATTACTGGTT+TGG - chr6_3:5598756-5598775 Msa0935140:intron 30.0%
TTTAACAATGTTTGTCTCTG+AGG + chr6_3:5598865-5598884 None:intergenic 30.0%
ACATTGTTAAAGAAACTGTG+GGG - chr6_3:5598874-5598893 Msa0935140:intron 30.0%
! ATAGGATGAATCCTTTTCAA+TGG - chr6_3:5598963-5598982 Msa0935140:intron 30.0%
!!! ATATTTTCAGTTTGTTGACG+GGG - chr6_3:5599091-5599110 Msa0935140:intron 30.0%
TTTCATACTCACGTATGATA+AGG + chr6_3:5599203-5599222 None:intergenic 30.0%
ATAACAATCTTTCCATCTTC+AGG + chr6_3:5599333-5599352 None:intergenic 30.0%
!! GATTGTTTCTGGTAAGAAAT+AGG - chr6_3:5599381-5599400 Msa0935140:intron 30.0%
AAACAGTAGACAAACTTAGA+CGG + chr6_3:5599417-5599436 None:intergenic 30.0%
AAGACTTATACGCCATTTAA+TGG - chr6_3:5599439-5599458 Msa0935140:intron 30.0%
AAGAGATTGCAAGAAAAGAA+AGG - chr6_3:5599555-5599574 Msa0935140:intron 30.0%
GTCATCAAAAATAAATGAGC+TGG - chr6_3:5599633-5599652 Msa0935140:intron 30.0%
CAAAGTTGACAATGTTTGTT+GGG - chr6_3:5600058-5600077 Msa0935140:intron 30.0%
!! TTTGATAGCTTGTTGAAAAG+AGG - chr6_3:5600090-5600109 Msa0935140:intron 30.0%
AGCTTGAATAAATTGAAGCA+TGG + chr6_3:5600207-5600226 None:intergenic 30.0%
GAATTACCGGTCATATTTAT+TGG - chr6_3:5600355-5600374 Msa0935140:intron 30.0%
CTTGTTCCAATAAATATGAC+CGG + chr6_3:5600364-5600383 None:intergenic 30.0%
!!! GTTTTATTTTTGTAGGTTGG+AGG + chr6_3:5600452-5600471 None:intergenic 30.0%
! AAAGTACCCAATACGGAAAT+AGG + chr6_3:5596731-5596750 None:intergenic 35.0%
!! GTTTGAGAAAGTACCCAATA+CGG + chr6_3:5596738-5596757 None:intergenic 35.0%
!!! TTTTTTTACTTAACTCCGCG+CGG - chr6_3:5596792-5596811 Msa0935140:three_prime_UTR 35.0%
! TTTTCGCAACATTGTTCAAC+GGG - chr6_3:5596924-5596943 Msa0935140:three_prime_UTR 35.0%
! TCAAAGTACTTCAACAATGC+TGG + chr6_3:5597153-5597172 None:intergenic 35.0%
TACTTTGATTGTACTCGTTC+TGG - chr6_3:5597165-5597184 Msa0935140:intron 35.0%
TGCATTTGTGTGAAAAGAGA+TGG - chr6_3:5597348-5597367 Msa0935140:three_prime_UTR 35.0%
ATTTGTGTGAAAAGAGATGG+TGG - chr6_3:5597351-5597370 Msa0935140:three_prime_UTR 35.0%
! ATTACTAGTGGCGTTTGTTT+TGG + chr6_3:5597449-5597468 None:intergenic 35.0%
TGAATTGAGGTGAGACAATA+AGG - chr6_3:5597666-5597685 Msa0935140:intron 35.0%
TTTGGTAGGACACACTTTAA+AGG - chr6_3:5597689-5597708 Msa0935140:intron 35.0%
TTCATAACAACTAGTTAGGG+AGG - chr6_3:5597746-5597765 Msa0935140:intron 35.0%
! GTTGTTTCATCTTCAGATTC+TGG - chr6_3:5597874-5597893 Msa0935140:intron 35.0%
!! GACTTTGCTTTTGATCGAAT+GGG - chr6_3:5597896-5597915 Msa0935140:intron 35.0%
ACTTATGGAGTTCCATCTAT+AGG - chr6_3:5598556-5598575 Msa0935140:intron 35.0%
AAGGCTCTTGTAAAAGATCA+AGG + chr6_3:5598614-5598633 None:intergenic 35.0%
ATGTCACAAGATGTCAAACA+TGG + chr6_3:5598742-5598761 None:intergenic 35.0%
CATTGTTAAAGAAACTGTGG+GGG - chr6_3:5598875-5598894 Msa0935140:intron 35.0%
ATTGTTAAAGAAACTGTGGG+GGG - chr6_3:5598876-5598895 Msa0935140:intron 35.0%
CTACAAGTAACTCTGCAAAA+CGG + chr6_3:5598998-5599017 None:intergenic 35.0%
CGACAGCTTCCTTTATAAAT+AGG + chr6_3:5599119-5599138 None:intergenic 35.0%
GCTGAAAATAATCAGTCACA+TGG + chr6_3:5599160-5599179 None:intergenic 35.0%
TACGTATTTAGGAGTTAGCT+TGG + chr6_3:5599246-5599265 None:intergenic 35.0%
AATACAGGGCTAGATTTACA+TGG - chr6_3:5599293-5599312 Msa0935140:intron 35.0%
ATACAGGGCTAGATTTACAT+GGG - chr6_3:5599294-5599313 Msa0935140:intron 35.0%
GAAAGATTGTTATGTTGCAC+AGG - chr6_3:5599340-5599359 Msa0935140:intron 35.0%
AACAGTAGACAAACTTAGAC+GGG + chr6_3:5599416-5599435 None:intergenic 35.0%
CTTTCTGCAAGACCATTAAA+TGG + chr6_3:5599454-5599473 None:intergenic 35.0%
! CATTTAATGGTCTTGCAGAA+AGG - chr6_3:5599452-5599471 Msa0935140:intron 35.0%
!!! CCACATTTTGGTTTTTCCTT+TGG + chr6_3:5599489-5599508 None:intergenic 35.0%
CCAAAGGAAAAACCAAAATG+TGG - chr6_3:5599486-5599505 Msa0935140:intron 35.0%
AGAAAAGAAAGGCAGCAATA+AGG - chr6_3:5599566-5599585 Msa0935140:intron 35.0%
GAAAAGAAAGGCAGCAATAA+GGG - chr6_3:5599567-5599586 Msa0935140:intron 35.0%
GGAAAATGAATTACGTGGTT+TGG - chr6_3:5599654-5599673 Msa0935140:intron 35.0%
TGGATCCAAGCATATTTGAA+GGG + chr6_3:5599979-5599998 None:intergenic 35.0%
ATGGATCCAAGCATATTTGA+AGG + chr6_3:5599980-5599999 None:intergenic 35.0%
GCAAAGTTGACAATGTTTGT+TGG - chr6_3:5600057-5600076 Msa0935140:intron 35.0%
!! AAAAGAGAAGTGCTATGAAG+AGG - chr6_3:5600128-5600147 Msa0935140:intron 35.0%
CTATGAAGAGGAATTGGTAA+GGG - chr6_3:5600140-5600159 Msa0935140:intron 35.0%
AATTGAAGCATGGAACATAC+AGG + chr6_3:5600197-5600216 None:intergenic 35.0%
! TAGGTTGGAGGATATAATTG+TGG + chr6_3:5600440-5600459 None:intergenic 35.0%
CAAAATTCAAAGAAACCGCG+CGG + chr6_3:5596810-5596829 None:intergenic 40.0%
!! TGGTGAATTTTTAGATGGGC+TGG - chr6_3:5596880-5596899 Msa0935140:three_prime_UTR 40.0%
ATGTTGCGAAAAACAACACG+AGG + chr6_3:5596916-5596935 None:intergenic 40.0%
GATGATGAAGAAGATACCTG+TGG + chr6_3:5596962-5596981 None:intergenic 40.0%
GGAGAAACTGTTACCATTGT+TGG - chr6_3:5597117-5597136 Msa0935140:intron 40.0%
ACTTCAACAATGCTGGATCA+TGG + chr6_3:5597146-5597165 None:intergenic 40.0%
TGACATGCAGCCATCAAAAT+AGG + chr6_3:5597317-5597336 None:intergenic 40.0%
CCTATCTATAGCACCAAAAC+AGG + chr6_3:5597382-5597401 None:intergenic 40.0%
!!! CCTGTTTTGGTGCTATAGAT+AGG - chr6_3:5597379-5597398 Msa0935140:three_prime_UTR 40.0%
ACAGGACTCATAGCAAAGTT+TGG + chr6_3:5597517-5597536 None:intergenic 40.0%
TTGCTATGAGTCCTGTACTT+AGG - chr6_3:5597521-5597540 Msa0935140:three_prime_UTR 40.0%
TGAGGTGAGACAATAAGGTT+TGG - chr6_3:5597671-5597690 Msa0935140:intron 40.0%
! GTGAGACAATAAGGTTTGGT+AGG - chr6_3:5597675-5597694 Msa0935140:intron 40.0%
! CACACTTTAAAGGTCACAGT+TGG - chr6_3:5597699-5597718 Msa0935140:intron 40.0%
!! GGACTTTGCTTTTGATCGAA+TGG - chr6_3:5597895-5597914 Msa0935140:intron 40.0%
TCAAATAAGCCCATCCAAAC+AGG - chr6_3:5598293-5598312 Msa0935140:intron 40.0%
ATAGGATGATCAATCTGCTC+TGG + chr6_3:5598469-5598488 None:intergenic 40.0%
ACACATTCTGTGATCAGGAT+AGG + chr6_3:5598487-5598506 None:intergenic 40.0%
TGCATACACATTCTGTGATC+AGG + chr6_3:5598492-5598511 None:intergenic 40.0%
! ATCAAGGCTTTTGCCATTGT+GGG + chr6_3:5598598-5598617 None:intergenic 40.0%
!! CGCTTCTGTTTCTACTACTA+AGG + chr6_3:5598633-5598652 None:intergenic 40.0%
TCACTTAAGAATAGACCTGG+TGG + chr6_3:5598689-5598708 None:intergenic 40.0%
! CGTTTTGCAGAGTTACTTGT+AGG - chr6_3:5598996-5599015 Msa0935140:intron 40.0%
TAACACTTGACACATTTGGC+AGG + chr6_3:5599032-5599051 None:intergenic 40.0%
GACGGGGAACCTATTTATAA+AGG - chr6_3:5599107-5599126 Msa0935140:intron 40.0%
! AGCTTGGTAATAGCAAAAGC+AGG + chr6_3:5599230-5599249 None:intergenic 40.0%
GGCTAGATTTACATGGGAAA+AGG - chr6_3:5599300-5599319 Msa0935140:intron 40.0%
AAAGGTTGAAGACCTGAAGA+TGG - chr6_3:5599318-5599337 Msa0935140:intron 40.0%
TTCAGAAGCTCGATTGTTTC+TGG - chr6_3:5599370-5599389 Msa0935140:intron 40.0%
ACAGTAGACAAACTTAGACG+GGG + chr6_3:5599415-5599434 None:intergenic 40.0%
TAAGGGAGAAACAAGGTCAA+AGG - chr6_3:5599584-5599603 Msa0935140:intron 40.0%
GAGCTGGAAAATGAATTACG+TGG - chr6_3:5599649-5599668 Msa0935140:intron 40.0%
AGGACTGCACAACTTTACTA+CGG + chr6_3:5599777-5599796 None:intergenic 40.0%
TCACACCCTTCAAATATGCT+TGG - chr6_3:5599971-5599990 Msa0935140:CDS 40.0%
! GAAGTGCTATGAAGAGGAAT+TGG - chr6_3:5600134-5600153 Msa0935140:intron 40.0%
GCTATGAAGAGGAATTGGTA+AGG - chr6_3:5600139-5600158 Msa0935140:intron 40.0%
AGAGAGAGAGATTTAGCAGT+TGG - chr6_3:5600300-5600319 Msa0935140:intron 40.0%
GCGCTTCCTATTTCCGTATT+GGG - chr6_3:5596722-5596741 Msa0935140:three_prime_UTR 45.0%
TGGATCATGGTCACCAACAA+TGG + chr6_3:5597133-5597152 None:intergenic 45.0%
TCGCTTTCAAATGCTGATCG+TGG + chr6_3:5597192-5597211 None:intergenic 45.0%
TGATGGCTGCATGTCAGAAT+TGG - chr6_3:5597321-5597340 Msa0935140:three_prime_UTR 45.0%
TTTGATCGAATGGGTGCAAC+AGG - chr6_3:5597905-5597924 Msa0935140:intron 45.0%
CTCTAATGGTAGGTCCTGTT+TGG - chr6_3:5598023-5598042 Msa0935140:intron 45.0%
AAGTTGGAGCCTGTTTGGAT+GGG + chr6_3:5598305-5598324 None:intergenic 45.0%
AAAGTTGGAGCCTGTTTGGA+TGG + chr6_3:5598306-5598325 None:intergenic 45.0%
ATCCAAACAGGCTCCAACTT+TGG - chr6_3:5598305-5598324 Msa0935140:intron 45.0%
GTTTAGCAGCACACCAAAGT+TGG + chr6_3:5598321-5598340 None:intergenic 45.0%
! TGGTGTGCTGCTAAACTTAG+TGG - chr6_3:5598325-5598344 Msa0935140:intron 45.0%
! GCTAAACTTAGTGGATCGTG+TGG - chr6_3:5598334-5598353 Msa0935140:intron 45.0%
!! ATCGTGTGGTGTTGTCATCT+TGG - chr6_3:5598348-5598367 Msa0935140:intron 45.0%
!! TGGTGTTGTCATCTTGGACT+TGG - chr6_3:5598354-5598373 Msa0935140:intron 45.0%
ATCTTGGACTTGGAGTTCCT+TGG - chr6_3:5598364-5598383 Msa0935140:intron 45.0%
! GATCAAGGCTTTTGCCATTG+TGG + chr6_3:5598599-5598618 None:intergenic 45.0%
AAGATTGGGCATAAGGCAAC+AGG + chr6_3:5598664-5598683 None:intergenic 45.0%
! TAGACCTGGTGGTGAAGATT+GGG + chr6_3:5598678-5598697 None:intergenic 45.0%
! ATAGACCTGGTGGTGAAGAT+TGG + chr6_3:5598679-5598698 None:intergenic 45.0%
GGCAGAACTGACCATTGAAA+AGG + chr6_3:5598977-5598996 None:intergenic 45.0%
GGAAGCTGTCGCACTAATAT+TGG - chr6_3:5599128-5599147 Msa0935140:intron 45.0%
AGCTGTCGCACTAATATTGG+TGG - chr6_3:5599131-5599150 Msa0935140:intron 45.0%
CTTGCAATCTCTTCCTCAGA+AGG + chr6_3:5599548-5599567 None:intergenic 45.0%
GCAGCAATAAGGGAGAAACA+AGG - chr6_3:5599577-5599596 Msa0935140:intron 45.0%
AGGTCAAAGGTTGCGAGAAA+TGG - chr6_3:5599597-5599616 Msa0935140:intron 45.0%
ATAGCCAGTCTCTGCTAAGA+AGG + chr6_3:5599728-5599747 None:intergenic 45.0%
GGACTGCACAACTTTACTAC+GGG + chr6_3:5599776-5599795 None:intergenic 45.0%
GTAAAGTTGTGCAGTCCTTG+CGG - chr6_3:5599779-5599798 Msa0935140:intron 45.0%
CAGTCCTTGCGGAAACAAAA+AGG - chr6_3:5599790-5599809 Msa0935140:intron 45.0%
!! CTCACCTTTTTGTTTCCGCA+AGG + chr6_3:5599797-5599816 None:intergenic 45.0%
! TTGAAGGGTGTGATGTGACA+GGG + chr6_3:5599964-5599983 None:intergenic 45.0%
! TTTGAAGGGTGTGATGTGAC+AGG + chr6_3:5599965-5599984 None:intergenic 45.0%
AAGAGGAATTGGTAAGGGAG+AGG - chr6_3:5600145-5600164 Msa0935140:intron 45.0%
GGCGCTTCCTATTTCCGTAT+TGG - chr6_3:5596721-5596740 Msa0935140:three_prime_UTR 50.0%
!! GATGGTGGTGCTTCCTGTTT+TGG - chr6_3:5597366-5597385 Msa0935140:three_prime_UTR 50.0%
GAATGGGTGCAACAGGATCA+GGG - chr6_3:5597912-5597931 Msa0935140:intron 50.0%
AATGGGTGCAACAGGATCAG+GGG - chr6_3:5597913-5597932 Msa0935140:intron 50.0%
GGGTATGGCCATGAGTAAAG+TGG - chr6_3:5597933-5597952 Msa0935140:CDS 50.0%
CACCAAAGTTGGAGCCTGTT+TGG + chr6_3:5598310-5598329 None:intergenic 50.0%
GGTGGTGAAGATTGGGCATA+AGG + chr6_3:5598671-5598690 None:intergenic 50.0%
TATGCCCAATCTTCACCACC+AGG - chr6_3:5598671-5598690 Msa0935140:intron 50.0%
GGTCTTGCAGAAAGGAGCTA+AGG - chr6_3:5599460-5599479 Msa0935140:intron 50.0%
CTTGCAGAAAGGAGCTAAGG+AGG - chr6_3:5599463-5599482 Msa0935140:intron 50.0%
AAAGGAGCTAAGGAGGCCAA+AGG - chr6_3:5599470-5599489 Msa0935140:intron 50.0%
CAAAATGTGGCAGCTCCCTT+GGG - chr6_3:5599499-5599518 Msa0935140:intron 50.0%
GCAATCTCTTCCTCAGAAGG+AGG + chr6_3:5599545-5599564 None:intergenic 50.0%
AAGGTTGCGAGAAATGGCTG+AGG - chr6_3:5599603-5599622 Msa0935140:intron 50.0%
CGATCCTTCTTAGCAGAGAC+TGG - chr6_3:5599721-5599740 Msa0935140:intron 50.0%
! CAGAGACTGGCTATGTCTCT+AGG - chr6_3:5599734-5599753 Msa0935140:intron 50.0%
CATCCCTGATGACATGCTGA+CGG - chr6_3:5599873-5599892 Msa0935140:intron 50.0%
GGAGAGGAAACAACAGCTAG+AGG - chr6_3:5600161-5600180 Msa0935140:intron 50.0%
GAGGAAACAACAGCTAGAGG+AGG - chr6_3:5600164-5600183 Msa0935140:intron 50.0%
AACAACCCACCCGATCGTGT+CGG - chr6_3:5596679-5596698 Msa0935140:three_prime_UTR 55.0%
CGAATGGGTGCAACAGGATC+AGG - chr6_3:5597911-5597930 Msa0935140:intron 55.0%
GTGCAACAGGATCAGGGGTA+TGG - chr6_3:5597918-5597937 Msa0935140:intron 55.0%
! CCAAGGGAGCTGCCACATTT+TGG + chr6_3:5599501-5599520 None:intergenic 55.0%
CCAAAATGTGGCAGCTCCCT+TGG - chr6_3:5599498-5599517 Msa0935140:intron 55.0%
TCAGAAGGAGGCTCCTCAAC+TGG + chr6_3:5599533-5599552 None:intergenic 55.0%
AGTTGAGGAGCCTCCTTCTG+AGG - chr6_3:5599532-5599551 Msa0935140:intron 55.0%
GAGAAATGGCTGAGGCGAAG+AGG - chr6_3:5599611-5599630 Msa0935140:intron 55.0%
! GACTGGCTATGTCTCTAGGC+AGG - chr6_3:5599738-5599757 Msa0935140:intron 55.0%
TTCTCAGTAGACGCAGCGTC+AGG + chr6_3:5599838-5599857 None:intergenic 55.0%
CTGCCGTCAGCATGTCATCA+GGG + chr6_3:5599879-5599898 None:intergenic 55.0%
TCTGCCGTCAGCATGTCATC+AGG + chr6_3:5599880-5599899 None:intergenic 55.0%
ACAACCCACCCGATCGTGTC+GGG - chr6_3:5596680-5596699 Msa0935140:three_prime_UTR 60.0%
CAACTGGAGGTGGCACCCAA+GGG + chr6_3:5599517-5599536 None:intergenic 60.0%
TCAACTGGAGGTGGCACCCA+AGG + chr6_3:5599518-5599537 None:intergenic 60.0%
! TTGGGTGCCACCTCCAGTTG+AGG - chr6_3:5599517-5599536 Msa0935140:intron 60.0%
GAAGGAGGCTCCTCAACTGG+AGG + chr6_3:5599530-5599549 None:intergenic 60.0%
TGACATGCTGACGGCAGAGC+AGG - chr6_3:5599882-5599901 Msa0935140:intron 60.0%
ATGCTGACGGCAGAGCAGGT+GGG - chr6_3:5599886-5599905 Msa0935140:intron 60.0%
CAACCCACCCGATCGTGTCG+GGG - chr6_3:5596681-5596700 Msa0935140:three_prime_UTR 65.0%
ACGGTGAGCCCCGACACGAT+CGG + chr6_3:5596692-5596711 None:intergenic 65.0%
CGGGCGCGACATAAAGCCAC+AGG - chr6_3:5596943-5596962 Msa0935140:three_prime_UTR 65.0%
GGAGGCTCCTCAACTGGAGG+TGG + chr6_3:5599527-5599546 None:intergenic 65.0%
CATGCTGACGGCAGAGCAGG+TGG - chr6_3:5599885-5599904 Msa0935140:intron 65.0%
GAGCCCCGACACGATCGGGT+GGG + chr6_3:5596687-5596706 None:intergenic 70.0%
TGAGCCCCGACACGATCGGG+TGG + chr6_3:5596688-5596707 None:intergenic 70.0%
CGGTGAGCCCCGACACGATC+GGG + chr6_3:5596691-5596710 None:intergenic 70.0%
GTGTCGGGGCTCACCGTGCT+GGG - chr6_3:5596695-5596714 Msa0935140:three_prime_UTR 70.0%
TGTCGGGGCTCACCGTGCTG+GGG - chr6_3:5596696-5596715 Msa0935140:three_prime_UTR 70.0%
!! AGGAAGCGCCAGCCCCAGCA+CGG + chr6_3:5596711-5596730 None:intergenic 70.0%
CGTGTCGGGGCTCACCGTGC+TGG - chr6_3:5596694-5596713 Msa0935140:three_prime_UTR 75.0%
! GGGGCTCACCGTGCTGGGGC+TGG - chr6_3:5596700-5596719 Msa0935140:three_prime_UTR 80.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 5596616 5600522 5596616 ID=Msa0935140;Name=Msa0935140
chr6_3 mRNA 5596616 5600522 5596616 ID=Msa0935140-mRNA-1;Parent=Msa0935140;Name=Msa0935140-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0.71|0.75|1|0.85|0.75|8|742|226
chr6_3 exon 5596616 5597040 5596616 ID=Msa0935140-mRNA-1:exon:1704;Parent=Msa0935140-mRNA-1
chr6_3 exon 5597235 5597668 5597235 ID=Msa0935140-mRNA-1:exon:1703;Parent=Msa0935140-mRNA-1
chr6_3 exon 5597754 5597838 5597754 ID=Msa0935140-mRNA-1:exon:1702;Parent=Msa0935140-mRNA-1
chr6_3 exon 5597928 5598037 5597928 ID=Msa0935140-mRNA-1:exon:1701;Parent=Msa0935140-mRNA-1
chr6_3 exon 5599204 5599248 5599204 ID=Msa0935140-mRNA-1:exon:1700;Parent=Msa0935140-mRNA-1
chr6_3 exon 5599896 5600020 5599896 ID=Msa0935140-mRNA-1:exon:1699;Parent=Msa0935140-mRNA-1
chr6_3 exon 5600174 5600244 5600174 ID=Msa0935140-mRNA-1:exon:1698;Parent=Msa0935140-mRNA-1
chr6_3 exon 5600395 5600522 5600395 ID=Msa0935140-mRNA-1:exon:1697;Parent=Msa0935140-mRNA-1
chr6_3 CDS 5600395 5600522 5600395 ID=Msa0935140-mRNA-1:cds;Parent=Msa0935140-mRNA-1
chr6_3 CDS 5600174 5600244 5600174 ID=Msa0935140-mRNA-1:cds;Parent=Msa0935140-mRNA-1
chr6_3 CDS 5599896 5600020 5599896 ID=Msa0935140-mRNA-1:cds;Parent=Msa0935140-mRNA-1
chr6_3 CDS 5599204 5599248 5599204 ID=Msa0935140-mRNA-1:cds;Parent=Msa0935140-mRNA-1
chr6_3 CDS 5597928 5598037 5597928 ID=Msa0935140-mRNA-1:cds;Parent=Msa0935140-mRNA-1
chr6_3 CDS 5597754 5597838 5597754 ID=Msa0935140-mRNA-1:cds;Parent=Msa0935140-mRNA-1
chr6_3 CDS 5597552 5597668 5597552 ID=Msa0935140-mRNA-1:cds;Parent=Msa0935140-mRNA-1
chr6_3 three_prime_UTR 5597235 5597551 5597235 ID=Msa0935140-mRNA-1:three_prime_utr;Parent=Msa0935140-mRNA-1
chr6_3 three_prime_UTR 5596616 5597040 5596616 ID=Msa0935140-mRNA-1:three_prime_utr;Parent=Msa0935140-mRNA-1
Gene Sequence

>Msa0935140

ATGTCCCTACAAAGTACAAACCGATTTAACTTTGTCAACAAAAAAGGAACATTAGTTGACTTCAACAACCCACCCGATCGTGTCGGGGCTCACCGTGCTGGGGCTGGCGCTTCCTATTTCCGTATTGGATGGGCTGGTGAGTCTGAACCTCGTGTTGTTTTTCGCAACATTGTTCAACGGGCGCGACATAAAGCCACAGGAGAAACTGTTACCATTGTTGGTGACCATGATCCAGCATTGTTGAAGTACTTTGATTGTACTCGTTCTGGACCACGATCAGCATTTGAAAGCGATGTTGTTTTTCAGTTTGAAATAAAGGAATATATTCTGGACTTTGCTTTTGATCGAATGGGTGCAACAGGATCAGGGTTTGTTGACGGGGAACCTATTTATAAAGGAAGCTGTCGCACTAATATTGGTGGATTCCATGTGACTGATTATTTTCAGCGACTTCTTTCACTTAAATATCCTTATCATACGGCTAGATTTACATGGGAAAAGGTTGAAGACCTGAAGATGGAAAGATTGTTATGTTGCACAGGACTATGTTTCAGAAGCTCGATTAAAGGAGCTAAGGAGGCCAAAGGAAAAACCAAAATGTGGCAGCTCCCTTGGGTGCCACCTCCAGTTGAGGAGCCTCCTTCTGAGGAAGAGATTGCAAGAAAAGAAAGGCAGCAATAA
Protein sequence

>Msa0935140

MSLQSTNRFNFVNKKGTLVDFNNPPDRVGAHRAGAGASYFRIGWAGESEPRVVFRNIVQRARHKATGETVTIVGDHDPALLKYFDCTRSGPRSAFESDVVFQFEIKEYILDFAFDRMGATGSGFVDGEPIYKGSCRTNIGGFHVTDYFQRLLSLKYPYHTARFTWEKVEDLKMERLLCCTGLCFRSSIKGAKEAKGKTKMWQLPWVPPPVEEPPSEEEIARKERQQ*