AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0935060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0935060 MtrunA17_Chr3g0111971 87.379 103 11 1 36 138 27 127 1.90e-54 185
Msa0935060 MtrunA17_Chr3g0111971 82.178 101 16 1 124 222 186 286 1.87e-38 141
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0935060 AT3G12380.1 64.844 128 42 2 11 138 3 127 3.45e-48 168
Msa0935060 AT3G12380.1 66.990 103 28 2 124 222 186 286 3.53e-40 146
Msa0935060 AT3G12380.2 61.940 134 42 3 11 138 3 133 7.23e-46 162
Msa0935060 AT3G12380.2 66.990 103 28 2 124 222 192 292 3.65e-40 146

Find 94 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTACCGGTCATATTTAT+TGG 0.189709 6_3:-5425870 Msa0935060:three_prime_UTR
CGACAGCTTCCTTTATAAAT+AGG 0.192795 6_3:+5427105 None:intergenic
CAAAATTAAGCATCCTTAAA+TGG 0.211174 6_3:+5425711 None:intergenic
CCAAGGGAGCTGCCACATTT+TGG 0.253326 6_3:+5426723 None:intergenic
TACTTTGATTGTACTCGTTC+TGG 0.294611 6_3:-5429056 Msa0935060:CDS
GCGCTTCCTATTTCCGTATT+GGG 0.301969 6_3:-5429499 Msa0935060:intron
CGTGTCGGGGCTCACCGTGC+TGG 0.313800 6_3:-5429527 Msa0935060:CDS
GGCGCTTCCTATTTCCGTAT+TGG 0.315333 6_3:-5429500 Msa0935060:CDS
GGAAAATGAATTACGTGGTT+TGG 0.322105 6_3:-5426567 Msa0935060:three_prime_UTR
AAAGTACCCAATACGGAAAT+AGG 0.324181 6_3:+5429493 None:intergenic
GAAAAGAAAGGCAGCAATAA+GGG 0.331676 6_3:-5426654 Msa0935060:exon
CGAATGGGTGCAACAGGATC+AGG 0.331988 6_3:-5428310 Msa0935060:CDS
AGAAAAGAAAGGCAGCAATA+AGG 0.339186 6_3:-5426655 Msa0935060:exon
CCAAAATGTGGCAGCTCCCT+TGG 0.342410 6_3:-5426723 Msa0935060:CDS
GGGGCTCACCGTGCTGGGGC+TGG 0.355011 6_3:-5429521 Msa0935060:CDS
GACGGGGAACCTATTTATAA+AGG 0.363787 6_3:-5427114 Msa0935060:CDS
GGAAGCTGTCGCACTAATAT+TGG 0.366748 6_3:-5427093 Msa0935060:CDS
GAAGTGCTATGAAGAGGAAT+TGG 0.378574 6_3:-5426087 Msa0935060:three_prime_UTR
TAGTTTAGTAAATGAATTAC+CGG 0.380279 6_3:-5425883 Msa0935060:three_prime_UTR
TCAGAAGGAGGCTCCTCAAC+TGG 0.380356 6_3:+5426691 None:intergenic
CAGTCCTTGCGGAAACAAAA+AGG 0.381377 6_3:-5426431 Msa0935060:three_prime_UTR
ACAACCCACCCGATCGTGTC+GGG 0.384348 6_3:-5429541 Msa0935060:CDS
ATAACAATCTTTCCATCTTC+AGG 0.387111 6_3:+5426891 None:intergenic
TTTGATCGAATGGGTGCAAC+AGG 0.394008 6_3:-5428316 Msa0935060:CDS
TTTGATAGCTTGTTGAAAAG+AGG 0.403014 6_3:-5426131 Msa0935060:intron
AGGTCAAAGGTTGCGAGAAA+TGG 0.404209 6_3:-5426624 Msa0935060:three_prime_UTR
GGAGAAACTGTTACCATTGT+TGG 0.413207 6_3:-5429104 Msa0935060:CDS
TCTGCCGTCAGCATGTCATC+AGG 0.414265 6_3:+5426344 None:intergenic
AGAGAGAGAGATTTAGCAGT+TGG 0.416915 6_3:-5425925 Msa0935060:three_prime_UTR
TAAATCGGTTTGTACTTTGT+AGG 0.443447 6_3:+5429600 None:intergenic
TTCTCAGTAGACGCAGCGTC+AGG 0.462710 6_3:+5426386 None:intergenic
CTTGCAATCTCTTCCTCAGA+AGG 0.464435 6_3:+5426676 None:intergenic
GGCTAGATTTACATGGGAAA+AGG 0.468160 6_3:-5426921 Msa0935060:CDS
AAATCGGTTTGTACTTTGTA+GGG 0.470081 6_3:+5429601 None:intergenic
CAAAATGTGGCAGCTCCCTT+GGG 0.477303 6_3:-5426722 Msa0935060:CDS
CGGTGAGCCCCGACACGATC+GGG 0.487843 6_3:+5429533 None:intergenic
TCAAAGTACTTCAACAATGC+TGG 0.488489 6_3:+5429071 None:intergenic
TTTCATACTCACGTATGATA+AGG 0.501112 6_3:+5427021 None:intergenic
ATAGCCAGTCTCTGCTAAGA+AGG 0.501279 6_3:+5426496 None:intergenic
TCAACTGGAGGTGGCACCCA+AGG 0.504173 6_3:+5426706 None:intergenic
GAAAGATTGTTATGTTGCAC+AGG 0.505276 6_3:-5426881 Msa0935060:CDS
GCTATGAAGAGGAATTGGTA+AGG 0.508107 6_3:-5426082 Msa0935060:three_prime_UTR
AAGAGGAATTGGTAAGGGAG+AGG 0.512761 6_3:-5426076 Msa0935060:three_prime_UTR
TTGGGTGCCACCTCCAGTTG+AGG 0.515845 6_3:-5426704 Msa0935060:CDS
CGATCCTTCTTAGCAGAGAC+TGG 0.517740 6_3:-5426500 Msa0935060:three_prime_UTR
AATACAGGGCTAGATTTACA+TGG 0.523587 6_3:-5426928 Msa0935060:intron
ACTTCAACAATGCTGGATCA+TGG 0.526588 6_3:+5429078 None:intergenic
AATTGAAGCATGGAACATAC+AGG 0.529482 6_3:+5426027 None:intergenic
CAGAGACTGGCTATGTCTCT+AGG 0.531810 6_3:-5426487 Msa0935060:three_prime_UTR
AAAAGAGAAGTGCTATGAAG+AGG 0.534586 6_3:-5426093 Msa0935060:three_prime_UTR
GACTGGCTATGTCTCTAGGC+AGG 0.538143 6_3:-5426483 Msa0935060:three_prime_UTR
AAAGGTTGAAGACCTGAAGA+TGG 0.539338 6_3:-5426903 Msa0935060:CDS
AGGACTGCACAACTTTACTA+CGG 0.540370 6_3:+5426447 None:intergenic
GGAGGCTCCTCAACTGGAGG+TGG 0.543167 6_3:+5426697 None:intergenic
AGCTGTCGCACTAATATTGG+TGG 0.543175 6_3:-5427090 Msa0935060:CDS
TGACATGCTGACGGCAGAGC+AGG 0.546133 6_3:-5426339 Msa0935060:intron
AAGAGATTGCAAGAAAAGAA+AGG 0.547623 6_3:-5426666 Msa0935060:CDS
ACGGTGAGCCCCGACACGAT+CGG 0.558422 6_3:+5429532 None:intergenic
CTTGTTCCAATAAATATGAC+CGG 0.563798 6_3:+5425864 None:intergenic
TAGGTTGGAGGATATAATTG+TGG 0.569810 6_3:+5425788 None:intergenic
CTATGAAGAGGAATTGGTAA+GGG 0.573363 6_3:-5426081 Msa0935060:three_prime_UTR
TGAGCCCCGACACGATCGGG+TGG 0.575680 6_3:+5429536 None:intergenic
CAACTGGAGGTGGCACCCAA+GGG 0.576342 6_3:+5426707 None:intergenic
GTTTGAGAAAGTACCCAATA+CGG 0.582666 6_3:+5429486 None:intergenic
AGTTGAGGAGCCTCCTTCTG+AGG 0.582751 6_3:-5426689 Msa0935060:CDS
GAATGGGTGCAACAGGATCA+GGG 0.585195 6_3:-5428309 Msa0935060:CDS
AACAACCCACCCGATCGTGT+CGG 0.591970 6_3:-5429542 Msa0935060:CDS
TGTCGGGGCTCACCGTGCTG+GGG 0.593810 6_3:-5429525 Msa0935060:CDS
GGTCTTGCAGAAAGGAGCTA+AGG 0.595543 6_3:-5426761 Msa0935060:intron
AGCTTGAATAAATTGAAGCA+TGG 0.599376 6_3:+5426017 None:intergenic
TAAGGGAGAAACAAGGTCAA+AGG 0.600031 6_3:-5426637 Msa0935060:three_prime_UTR
GGACTGCACAACTTTACTAC+GGG 0.602389 6_3:+5426448 None:intergenic
CTGCCGTCAGCATGTCATCA+GGG 0.602597 6_3:+5426345 None:intergenic
CAACCCACCCGATCGTGTCG+GGG 0.604755 6_3:-5429540 Msa0935060:CDS
AAAGGAGCTAAGGAGGCCAA+AGG 0.605406 6_3:-5426751 Msa0935060:CDS
TGGATCATGGTCACCAACAA+TGG 0.605508 6_3:+5429091 None:intergenic
CTTGCAGAAAGGAGCTAAGG+AGG 0.614499 6_3:-5426758 Msa0935060:intron
GCAATCTCTTCCTCAGAAGG+AGG 0.622040 6_3:+5426679 None:intergenic
AGGAAGCGCCAGCCCCAGCA+CGG 0.622653 6_3:+5429513 None:intergenic
CGGGCGCGACATAAAGCCAC+AGG 0.623244 6_3:-5429278 Msa0935060:intron
GGAGAGGAAACAACAGCTAG+AGG 0.635531 6_3:-5426060 Msa0935060:three_prime_UTR
GAGGAAACAACAGCTAGAGG+AGG 0.641074 6_3:-5426057 Msa0935060:three_prime_UTR
GAAGGAGGCTCCTCAACTGG+AGG 0.645561 6_3:+5426694 None:intergenic
GAGAAATGGCTGAGGCGAAG+AGG 0.646132 6_3:-5426610 Msa0935060:three_prime_UTR
GCTGAAAATAATCAGTCACA+TGG 0.648180 6_3:+5427064 None:intergenic
AAGGTTGCGAGAAATGGCTG+AGG 0.651418 6_3:-5426618 Msa0935060:three_prime_UTR
GAGCCCCGACACGATCGGGT+GGG 0.652167 6_3:+5429537 None:intergenic
GTAAAGTTGTGCAGTCCTTG+CGG 0.652419 6_3:-5426442 Msa0935060:three_prime_UTR
AATGGGTGCAACAGGATCAG+GGG 0.654606 6_3:-5428308 Msa0935060:intron
ATACAGGGCTAGATTTACAT+GGG 0.656838 6_3:-5426927 Msa0935060:intron
TCGCTTTCAAATGCTGATCG+TGG 0.660583 6_3:+5429032 None:intergenic
CATCCCTGATGACATGCTGA+CGG 0.663450 6_3:-5426348 Msa0935060:three_prime_UTR
GCAGCAATAAGGGAGAAACA+AGG 0.705579 6_3:-5426644 Msa0935060:exon
GAGCTGGAAAATGAATTACG+TGG 0.728785 6_3:-5426572 Msa0935060:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATAAATTTTTAAATAAAT+AGG - chr6_3:5428054-5428073 Msa0935060:intron 0.0%
!! AAAAACTAAACAATAAAAAA+TGG - chr6_3:5428022-5428041 Msa0935060:intron 10.0%
!!! ATTTTTTTGAGATGAAATTA+GGG + chr6_3:5425869-5425888 None:intergenic 15.0%
!!! AATTTTTTTGAGATGAAATT+AGG + chr6_3:5425870-5425889 None:intergenic 15.0%
!!! TTTTTATTTGAATTGTTGTT+GGG - chr6_3:5426160-5426179 Msa0935060:intron 15.0%
!! AATATGAATAATTTCTAACT+AGG - chr6_3:5426575-5426594 Msa0935060:three_prime_UTR 15.0%
!! ATATGAATAATTTCTAACTA+GGG - chr6_3:5426576-5426595 Msa0935060:three_prime_UTR 15.0%
!! CAATATTCAAAGAAATTATT+TGG + chr6_3:5429133-5429152 None:intergenic 15.0%
!!! TTTTGTTGACAAAGTTAAAT+CGG + chr6_3:5425748-5425767 None:intergenic 20.0%
!!! TTTAACTTTGTCAACAAAAA+AGG - chr6_3:5425749-5425768 Msa0935060:three_prime_UTR 20.0%
!!! AATTTTGAATTTTCTCATCA+TGG - chr6_3:5425932-5425951 Msa0935060:three_prime_UTR 20.0%
!!! ATTTTGAATTTTCTCATCAT+GGG - chr6_3:5425933-5425952 Msa0935060:three_prime_UTR 20.0%
!!! TTTTTGGTGAATTTTTAGAT+GGG - chr6_3:5425985-5426004 Msa0935060:three_prime_UTR 20.0%
!!! GTTTTTATTTGAATTGTTGT+TGG - chr6_3:5426159-5426178 Msa0935060:intron 20.0%
!! TTGGGATATTGAATAATTTA+TGG - chr6_3:5426178-5426197 Msa0935060:intron 20.0%
!!! TGTTTTTCAGTTTGAAATAA+AGG - chr6_3:5426324-5426343 Msa0935060:intron 20.0%
!! GAAATTATTCATATTACTAG+TGG + chr6_3:5426570-5426589 None:intergenic 20.0%
!! TACTTGTTCATACTATAAAA+AGG + chr6_3:5426718-5426737 None:intergenic 20.0%
!! TTATAGTATGAACAAGTAAA+TGG - chr6_3:5426720-5426739 Msa0935060:CDS 20.0%
!! TCATTCATATATTTCATACA+AGG + chr6_3:5426758-5426777 None:intergenic 20.0%
!! TGAAATATATGAATGAATTG+AGG - chr6_3:5426762-5426781 Msa0935060:intron 20.0%
!! TGTACCATATTACATATTAT+GGG - chr6_3:5426878-5426897 Msa0935060:CDS 20.0%
!! TATTCCCATAATATGTAATA+TGG + chr6_3:5426885-5426904 None:intergenic 20.0%
!! TATTACATATTATGGGAATA+TGG - chr6_3:5426885-5426904 Msa0935060:CDS 20.0%
!! TCAGTAATATTAAACTCTAA+TGG - chr6_3:5427118-5427137 Msa0935060:CDS 20.0%
!! TAATATTAAACTCTAATGGT+AGG - chr6_3:5427122-5427141 Msa0935060:CDS 20.0%
!!! TTATATTTTCAGTTTGTTGA+CGG - chr6_3:5428198-5428217 Msa0935060:intron 20.0%
!! TCTTATTAAATTCTCAATAC+AGG - chr6_3:5428387-5428406 Msa0935060:intron 20.0%
!! CTTATTAAATTCTCAATACA+GGG - chr6_3:5428388-5428407 Msa0935060:intron 20.0%
!! TAGTTTAGTAAATGAATTAC+CGG - chr6_3:5429447-5429466 Msa0935060:intron 20.0%
! CATCTAAAAATTCACCAAAA+AGG + chr6_3:5425986-5426005 None:intergenic 25.0%
!!! CTTTTTGGTGAATTTTTAGA+TGG - chr6_3:5425984-5426003 Msa0935060:three_prime_UTR 25.0%
!! TTTTTAACATTATGTGACAC+AGG - chr6_3:5426205-5426224 Msa0935060:intron 25.0%
! TATACTCACATAATTGTAAC+AGG + chr6_3:5426364-5426383 None:intergenic 25.0%
! TGTTACAATTATGTGAGTAT+AGG - chr6_3:5426363-5426382 Msa0935060:three_prime_UTR 25.0%
!!! ATAGGAGGAATAGTTTTTTT+AGG - chr6_3:5426381-5426400 Msa0935060:three_prime_UTR 25.0%
! GATCATTAACTAAAAAACAC+AGG + chr6_3:5426531-5426550 None:intergenic 25.0%
! TAATTTCTAACTAGGGAAAT+AGG - chr6_3:5426583-5426602 Msa0935060:three_prime_UTR 25.0%
! AATATGTAAATCCTAAGTAC+AGG + chr6_3:5426644-5426663 None:intergenic 25.0%
!!! CATTTTAGTGTGATAGTTTT+AGG + chr6_3:5426667-5426686 None:intergenic 25.0%
! TAAGTTCATAACAACTAGTT+AGG - chr6_3:5426851-5426870 Msa0935060:intron 25.0%
! AAGTTCATAACAACTAGTTA+GGG - chr6_3:5426852-5426871 Msa0935060:intron 25.0%
! GTGTACCATATTACATATTA+TGG - chr6_3:5426877-5426896 Msa0935060:CDS 25.0%
! ATATGGTCATTAATGTACAT+TGG - chr6_3:5426902-5426921 Msa0935060:CDS 25.0%
!!! CATTAATGTACATTGGTTTA+AGG - chr6_3:5426909-5426928 Msa0935060:CDS 25.0%
! ATAAAAAACCACTTTACTCA+TGG + chr6_3:5427053-5427072 None:intergenic 25.0%
! ATGATATAAGCTGCAAATTA+TGG + chr6_3:5427165-5427184 None:intergenic 25.0%
!!! GAACTTTTTTTTGAGACTTA+TGG - chr6_3:5427650-5427669 Msa0935060:intron 25.0%
!! AAAGTATTAGTACCTATAGA+TGG + chr6_3:5427680-5427699 None:intergenic 25.0%
!!! TAATACTTTTTAACCCACAA+TGG - chr6_3:5427691-5427710 Msa0935060:intron 25.0%
! AAACATTGTTAAAGAAACTG+TGG - chr6_3:5427981-5428000 Msa0935060:intron 25.0%
! AACATTGTTAAAGAAACTGT+GGG - chr6_3:5427982-5428001 Msa0935060:intron 25.0%
! TTATTAACACTTGACACATT+TGG + chr6_3:5428145-5428164 None:intergenic 25.0%
!!! TATATTTTCAGTTTGTTGAC+GGG - chr6_3:5428199-5428218 Msa0935060:intron 25.0%
! GTAAGAAATAGTACGTATTT+AGG + chr6_3:5428366-5428385 None:intergenic 25.0%
!! GAATTTCTTTTGAATCAACT+TGG - chr6_3:5428785-5428804 Msa0935060:intron 25.0%
! ATTAAGACTATAAGCACATA+TGG + chr6_3:5429108-5429127 None:intergenic 25.0%
! TCTTTGAATATTGTTTACTG+TGG - chr6_3:5429140-5429159 Msa0935060:intron 25.0%
!!! CTTGTTTTATTTTTGTAGGT+TGG + chr6_3:5429560-5429579 None:intergenic 25.0%
! CAACCTACAAAAATAAAACA+AGG - chr6_3:5429558-5429577 Msa0935060:CDS 25.0%
!!! AGTCCTTGTTTTATTTTTGT+AGG + chr6_3:5429564-5429583 None:intergenic 25.0%
!! AAATCGGTTTGTACTTTGTA+GGG + chr6_3:5425732-5425751 None:intergenic 30.0%
!! TAAATCGGTTTGTACTTTGT+AGG + chr6_3:5425733-5425752 None:intergenic 30.0%
!!! CTTTAAAGTTCGTTCCTTTT+TGG - chr6_3:5425969-5425988 Msa0935060:three_prime_UTR 30.0%
! TTTTTCGCAACATTGTTCAA+CGG - chr6_3:5426032-5426051 Msa0935060:three_prime_UTR 30.0%
TACAATTATGTGAGTATAGG+AGG - chr6_3:5426366-5426385 Msa0935060:three_prime_UTR 30.0%
!! GATATAGTGACCTATTTTGA+TGG - chr6_3:5426413-5426432 Msa0935060:three_prime_UTR 30.0%
GTTATGAACTTATGAGCATA+TGG + chr6_3:5426843-5426862 None:intergenic 30.0%
ATTATGGTGTTTATCCAAAC+AGG + chr6_3:5427149-5427168 None:intergenic 30.0%
GAACTTATGAAAACAGCTTA+TGG + chr6_3:5427343-5427362 None:intergenic 30.0%
AAACATGTATTGCAAAACCA+AGG + chr6_3:5427493-5427512 None:intergenic 30.0%
! TTTTACTTCGAGATTGAACT+GGG + chr6_3:5427625-5427644 None:intergenic 30.0%
! ATTTTACTTCGAGATTGAAC+TGG + chr6_3:5427626-5427645 None:intergenic 30.0%
AGTTCAATCTCGAAGTAAAA+TGG - chr6_3:5427625-5427644 Msa0935060:intron 30.0%
! TTTTCACTTAAGAATAGACC+TGG + chr6_3:5427801-5427820 None:intergenic 30.0%
TCTTGTGACATAGTTATTAC+TGG - chr6_3:5427860-5427879 Msa0935060:intron 30.0%
! TGACATAGTTATTACTGGTT+TGG - chr6_3:5427865-5427884 Msa0935060:intron 30.0%
TTTAACAATGTTTGTCTCTG+AGG + chr6_3:5427974-5427993 None:intergenic 30.0%
ACATTGTTAAAGAAACTGTG+GGG - chr6_3:5427983-5428002 Msa0935060:intron 30.0%
! ATAGGATGAATCCTTTTCAA+TGG - chr6_3:5428072-5428091 Msa0935060:intron 30.0%
!!! ATATTTTCAGTTTGTTGACG+GGG - chr6_3:5428200-5428219 Msa0935060:intron 30.0%
TTTCATACTCACGTATGATA+AGG + chr6_3:5428312-5428331 None:intergenic 30.0%
ATAACAATCTTTCCATCTTC+AGG + chr6_3:5428442-5428461 None:intergenic 30.0%
!! GATTGTTTCTGGTAAGAAAT+AGG - chr6_3:5428490-5428509 Msa0935060:intron 30.0%
AAACAGTAGACAAACTTAGA+CGG + chr6_3:5428526-5428545 None:intergenic 30.0%
AAGACTTATACGCCATTTAA+TGG - chr6_3:5428548-5428567 Msa0935060:intron 30.0%
AAGAGATTGCAAGAAAAGAA+AGG - chr6_3:5428664-5428683 Msa0935060:intron 30.0%
GTCATCAAAAATAAATGAGC+TGG - chr6_3:5428742-5428761 Msa0935060:intron 30.0%
CAAAGTTGACAATGTTTGTT+GGG - chr6_3:5429167-5429186 Msa0935060:intron 30.0%
!! TTTGATAGCTTGTTGAAAAG+AGG - chr6_3:5429199-5429218 Msa0935060:intron 30.0%
AGCTTGAATAAATTGAAGCA+TGG + chr6_3:5429316-5429335 None:intergenic 30.0%
GAATTACCGGTCATATTTAT+TGG - chr6_3:5429460-5429479 Msa0935060:intron 30.0%
CTTGTTCCAATAAATATGAC+CGG + chr6_3:5429469-5429488 None:intergenic 30.0%
!!! GTTTTATTTTTGTAGGTTGG+AGG + chr6_3:5429557-5429576 None:intergenic 30.0%
! AAAGTACCCAATACGGAAAT+AGG + chr6_3:5425840-5425859 None:intergenic 35.0%
!! GTTTGAGAAAGTACCCAATA+CGG + chr6_3:5425847-5425866 None:intergenic 35.0%
!!! TTTTTTTACTTAACTCCGCG+CGG - chr6_3:5425901-5425920 Msa0935060:three_prime_UTR 35.0%
! TTTTCGCAACATTGTTCAAC+GGG - chr6_3:5426033-5426052 Msa0935060:three_prime_UTR 35.0%
! TCAAAGTACTTCAACAATGC+TGG + chr6_3:5426262-5426281 None:intergenic 35.0%
TACTTTGATTGTACTCGTTC+TGG - chr6_3:5426274-5426293 Msa0935060:intron 35.0%
TGCATTTGTGTGAAAAGAGA+TGG - chr6_3:5426457-5426476 Msa0935060:three_prime_UTR 35.0%
ATTTGTGTGAAAAGAGATGG+TGG - chr6_3:5426460-5426479 Msa0935060:three_prime_UTR 35.0%
! ATTACTAGTGGCGTTTGTTT+TGG + chr6_3:5426558-5426577 None:intergenic 35.0%
TGAATTGAGGTGAGACAATA+AGG - chr6_3:5426775-5426794 Msa0935060:intron 35.0%
TTTGGTAGGACACACTTTAA+AGG - chr6_3:5426798-5426817 Msa0935060:intron 35.0%
TTCATAACAACTAGTTAGGG+AGG - chr6_3:5426855-5426874 Msa0935060:intron 35.0%
! GTTGTTTCATCTTCAGATTC+TGG - chr6_3:5426983-5427002 Msa0935060:intron 35.0%
!! GACTTTGCTTTTGATCGAAT+GGG - chr6_3:5427005-5427024 Msa0935060:intron 35.0%
ACTTATGGAGTTCCATCTAT+AGG - chr6_3:5427665-5427684 Msa0935060:intron 35.0%
AAGGCTCTTGTAAAAGATCA+AGG + chr6_3:5427723-5427742 None:intergenic 35.0%
ATGTCACAAGATGTCAAACA+TGG + chr6_3:5427851-5427870 None:intergenic 35.0%
CATTGTTAAAGAAACTGTGG+GGG - chr6_3:5427984-5428003 Msa0935060:intron 35.0%
ATTGTTAAAGAAACTGTGGG+GGG - chr6_3:5427985-5428004 Msa0935060:intron 35.0%
CTACAAGTAACTCTGCAAAA+CGG + chr6_3:5428107-5428126 None:intergenic 35.0%
CGACAGCTTCCTTTATAAAT+AGG + chr6_3:5428228-5428247 None:intergenic 35.0%
GCTGAAAATAATCAGTCACA+TGG + chr6_3:5428269-5428288 None:intergenic 35.0%
TACGTATTTAGGAGTTAGCT+TGG + chr6_3:5428355-5428374 None:intergenic 35.0%
AATACAGGGCTAGATTTACA+TGG - chr6_3:5428402-5428421 Msa0935060:intron 35.0%
ATACAGGGCTAGATTTACAT+GGG - chr6_3:5428403-5428422 Msa0935060:intron 35.0%
GAAAGATTGTTATGTTGCAC+AGG - chr6_3:5428449-5428468 Msa0935060:intron 35.0%
AACAGTAGACAAACTTAGAC+GGG + chr6_3:5428525-5428544 None:intergenic 35.0%
CTTTCTGCAAGACCATTAAA+TGG + chr6_3:5428563-5428582 None:intergenic 35.0%
! CATTTAATGGTCTTGCAGAA+AGG - chr6_3:5428561-5428580 Msa0935060:intron 35.0%
!!! CCACATTTTGGTTTTTCCTT+TGG + chr6_3:5428598-5428617 None:intergenic 35.0%
CCAAAGGAAAAACCAAAATG+TGG - chr6_3:5428595-5428614 Msa0935060:intron 35.0%
AGAAAAGAAAGGCAGCAATA+AGG - chr6_3:5428675-5428694 Msa0935060:intron 35.0%
GAAAAGAAAGGCAGCAATAA+GGG - chr6_3:5428676-5428695 Msa0935060:intron 35.0%
GGAAAATGAATTACGTGGTT+TGG - chr6_3:5428763-5428782 Msa0935060:intron 35.0%
TGGATCCAAGCATATTTGAA+GGG + chr6_3:5429088-5429107 None:intergenic 35.0%
ATGGATCCAAGCATATTTGA+AGG + chr6_3:5429089-5429108 None:intergenic 35.0%
GCAAAGTTGACAATGTTTGT+TGG - chr6_3:5429166-5429185 Msa0935060:intron 35.0%
!! AAAAGAGAAGTGCTATGAAG+AGG - chr6_3:5429237-5429256 Msa0935060:intron 35.0%
CTATGAAGAGGAATTGGTAA+GGG - chr6_3:5429249-5429268 Msa0935060:intron 35.0%
AATTGAAGCATGGAACATAC+AGG + chr6_3:5429306-5429325 None:intergenic 35.0%
! TAGGTTGGAGGATATAATTG+TGG + chr6_3:5429545-5429564 None:intergenic 35.0%
CAAAATTCAAAGAAACCGCG+CGG + chr6_3:5425919-5425938 None:intergenic 40.0%
!! TGGTGAATTTTTAGATGGGC+TGG - chr6_3:5425989-5426008 Msa0935060:three_prime_UTR 40.0%
ATGTTGCGAAAAACAACACG+AGG + chr6_3:5426025-5426044 None:intergenic 40.0%
GATGATGAAGAAGATACCTG+TGG + chr6_3:5426071-5426090 None:intergenic 40.0%
GGAGAAACTGTTACCATTGT+TGG - chr6_3:5426226-5426245 Msa0935060:intron 40.0%
ACTTCAACAATGCTGGATCA+TGG + chr6_3:5426255-5426274 None:intergenic 40.0%
TGACATGCAGCCATCAAAAT+AGG + chr6_3:5426426-5426445 None:intergenic 40.0%
CCTATCTATAGCACCAAAAC+AGG + chr6_3:5426491-5426510 None:intergenic 40.0%
!!! CCTGTTTTGGTGCTATAGAT+AGG - chr6_3:5426488-5426507 Msa0935060:three_prime_UTR 40.0%
ACAGGACTCATAGCAAAGTT+TGG + chr6_3:5426626-5426645 None:intergenic 40.0%
TTGCTATGAGTCCTGTACTT+AGG - chr6_3:5426630-5426649 Msa0935060:three_prime_UTR 40.0%
TGAGGTGAGACAATAAGGTT+TGG - chr6_3:5426780-5426799 Msa0935060:intron 40.0%
! GTGAGACAATAAGGTTTGGT+AGG - chr6_3:5426784-5426803 Msa0935060:intron 40.0%
! CACACTTTAAAGGTCACAGT+TGG - chr6_3:5426808-5426827 Msa0935060:intron 40.0%
!! GGACTTTGCTTTTGATCGAA+TGG - chr6_3:5427004-5427023 Msa0935060:intron 40.0%
TCAAATAAGCCCATCCAAAC+AGG - chr6_3:5427402-5427421 Msa0935060:intron 40.0%
ATAGGATGATCAATCTGCTC+TGG + chr6_3:5427578-5427597 None:intergenic 40.0%
ACACATTCTGTGATCAGGAT+AGG + chr6_3:5427596-5427615 None:intergenic 40.0%
TGCATACACATTCTGTGATC+AGG + chr6_3:5427601-5427620 None:intergenic 40.0%
! ATCAAGGCTTTTGCCATTGT+GGG + chr6_3:5427707-5427726 None:intergenic 40.0%
!! CGCTTCTGTTTCTACTACTA+AGG + chr6_3:5427742-5427761 None:intergenic 40.0%
TCACTTAAGAATAGACCTGG+TGG + chr6_3:5427798-5427817 None:intergenic 40.0%
! CGTTTTGCAGAGTTACTTGT+AGG - chr6_3:5428105-5428124 Msa0935060:intron 40.0%
TAACACTTGACACATTTGGC+AGG + chr6_3:5428141-5428160 None:intergenic 40.0%
GACGGGGAACCTATTTATAA+AGG - chr6_3:5428216-5428235 Msa0935060:intron 40.0%
! AGCTTGGTAATAGCAAAAGC+AGG + chr6_3:5428339-5428358 None:intergenic 40.0%
GGCTAGATTTACATGGGAAA+AGG - chr6_3:5428409-5428428 Msa0935060:intron 40.0%
AAAGGTTGAAGACCTGAAGA+TGG - chr6_3:5428427-5428446 Msa0935060:intron 40.0%
TTCAGAAGCTCGATTGTTTC+TGG - chr6_3:5428479-5428498 Msa0935060:intron 40.0%
ACAGTAGACAAACTTAGACG+GGG + chr6_3:5428524-5428543 None:intergenic 40.0%
TAAGGGAGAAACAAGGTCAA+AGG - chr6_3:5428693-5428712 Msa0935060:intron 40.0%
GAGCTGGAAAATGAATTACG+TGG - chr6_3:5428758-5428777 Msa0935060:intron 40.0%
AGGACTGCACAACTTTACTA+CGG + chr6_3:5428886-5428905 None:intergenic 40.0%
TCACACCCTTCAAATATGCT+TGG - chr6_3:5429080-5429099 Msa0935060:CDS 40.0%
! GAAGTGCTATGAAGAGGAAT+TGG - chr6_3:5429243-5429262 Msa0935060:intron 40.0%
GCTATGAAGAGGAATTGGTA+AGG - chr6_3:5429248-5429267 Msa0935060:intron 40.0%
AGAGAGAGAGATTTAGCAGT+TGG - chr6_3:5429405-5429424 Msa0935060:intron 40.0%
GCGCTTCCTATTTCCGTATT+GGG - chr6_3:5425831-5425850 Msa0935060:three_prime_UTR 45.0%
TGGATCATGGTCACCAACAA+TGG + chr6_3:5426242-5426261 None:intergenic 45.0%
TCGCTTTCAAATGCTGATCG+TGG + chr6_3:5426301-5426320 None:intergenic 45.0%
TGATGGCTGCATGTCAGAAT+TGG - chr6_3:5426430-5426449 Msa0935060:three_prime_UTR 45.0%
TTTGATCGAATGGGTGCAAC+AGG - chr6_3:5427014-5427033 Msa0935060:intron 45.0%
CTCTAATGGTAGGTCCTGTT+TGG - chr6_3:5427132-5427151 Msa0935060:intron 45.0%
AAGTTGGAGCCTGTTTGGAT+GGG + chr6_3:5427414-5427433 None:intergenic 45.0%
AAAGTTGGAGCCTGTTTGGA+TGG + chr6_3:5427415-5427434 None:intergenic 45.0%
ATCCAAACAGGCTCCAACTT+TGG - chr6_3:5427414-5427433 Msa0935060:intron 45.0%
GTTTAGCAGCACACCAAAGT+TGG + chr6_3:5427430-5427449 None:intergenic 45.0%
! TGGTGTGCTGCTAAACTTAG+TGG - chr6_3:5427434-5427453 Msa0935060:intron 45.0%
! GCTAAACTTAGTGGATCGTG+TGG - chr6_3:5427443-5427462 Msa0935060:intron 45.0%
!! ATCGTGTGGTGTTGTCATCT+TGG - chr6_3:5427457-5427476 Msa0935060:intron 45.0%
!! TGGTGTTGTCATCTTGGACT+TGG - chr6_3:5427463-5427482 Msa0935060:intron 45.0%
ATCTTGGACTTGGAGTTCCT+TGG - chr6_3:5427473-5427492 Msa0935060:intron 45.0%
! GATCAAGGCTTTTGCCATTG+TGG + chr6_3:5427708-5427727 None:intergenic 45.0%
AAGATTGGGCATAAGGCAAC+AGG + chr6_3:5427773-5427792 None:intergenic 45.0%
! TAGACCTGGTGGTGAAGATT+GGG + chr6_3:5427787-5427806 None:intergenic 45.0%
! ATAGACCTGGTGGTGAAGAT+TGG + chr6_3:5427788-5427807 None:intergenic 45.0%
GGCAGAACTGACCATTGAAA+AGG + chr6_3:5428086-5428105 None:intergenic 45.0%
GGAAGCTGTCGCACTAATAT+TGG - chr6_3:5428237-5428256 Msa0935060:intron 45.0%
AGCTGTCGCACTAATATTGG+TGG - chr6_3:5428240-5428259 Msa0935060:intron 45.0%
CTTGCAATCTCTTCCTCAGA+AGG + chr6_3:5428657-5428676 None:intergenic 45.0%
GCAGCAATAAGGGAGAAACA+AGG - chr6_3:5428686-5428705 Msa0935060:intron 45.0%
AGGTCAAAGGTTGCGAGAAA+TGG - chr6_3:5428706-5428725 Msa0935060:intron 45.0%
ATAGCCAGTCTCTGCTAAGA+AGG + chr6_3:5428837-5428856 None:intergenic 45.0%
GGACTGCACAACTTTACTAC+GGG + chr6_3:5428885-5428904 None:intergenic 45.0%
GTAAAGTTGTGCAGTCCTTG+CGG - chr6_3:5428888-5428907 Msa0935060:intron 45.0%
CAGTCCTTGCGGAAACAAAA+AGG - chr6_3:5428899-5428918 Msa0935060:intron 45.0%
!! CTCACCTTTTTGTTTCCGCA+AGG + chr6_3:5428906-5428925 None:intergenic 45.0%
! TTGAAGGGTGTGATGTGACA+GGG + chr6_3:5429073-5429092 None:intergenic 45.0%
! TTTGAAGGGTGTGATGTGAC+AGG + chr6_3:5429074-5429093 None:intergenic 45.0%
AAGAGGAATTGGTAAGGGAG+AGG - chr6_3:5429254-5429273 Msa0935060:intron 45.0%
GGCGCTTCCTATTTCCGTAT+TGG - chr6_3:5425830-5425849 Msa0935060:three_prime_UTR 50.0%
!! GATGGTGGTGCTTCCTGTTT+TGG - chr6_3:5426475-5426494 Msa0935060:three_prime_UTR 50.0%
GAATGGGTGCAACAGGATCA+GGG - chr6_3:5427021-5427040 Msa0935060:intron 50.0%
AATGGGTGCAACAGGATCAG+GGG - chr6_3:5427022-5427041 Msa0935060:intron 50.0%
GGGTATGGCCATGAGTAAAG+TGG - chr6_3:5427042-5427061 Msa0935060:CDS 50.0%
CACCAAAGTTGGAGCCTGTT+TGG + chr6_3:5427419-5427438 None:intergenic 50.0%
GGTGGTGAAGATTGGGCATA+AGG + chr6_3:5427780-5427799 None:intergenic 50.0%
TATGCCCAATCTTCACCACC+AGG - chr6_3:5427780-5427799 Msa0935060:intron 50.0%
GGTCTTGCAGAAAGGAGCTA+AGG - chr6_3:5428569-5428588 Msa0935060:intron 50.0%
CTTGCAGAAAGGAGCTAAGG+AGG - chr6_3:5428572-5428591 Msa0935060:intron 50.0%
AAAGGAGCTAAGGAGGCCAA+AGG - chr6_3:5428579-5428598 Msa0935060:intron 50.0%
CAAAATGTGGCAGCTCCCTT+GGG - chr6_3:5428608-5428627 Msa0935060:intron 50.0%
GCAATCTCTTCCTCAGAAGG+AGG + chr6_3:5428654-5428673 None:intergenic 50.0%
AAGGTTGCGAGAAATGGCTG+AGG - chr6_3:5428712-5428731 Msa0935060:intron 50.0%
CGATCCTTCTTAGCAGAGAC+TGG - chr6_3:5428830-5428849 Msa0935060:intron 50.0%
! CAGAGACTGGCTATGTCTCT+AGG - chr6_3:5428843-5428862 Msa0935060:intron 50.0%
CATCCCTGATGACATGCTGA+CGG - chr6_3:5428982-5429001 Msa0935060:intron 50.0%
GGAGAGGAAACAACAGCTAG+AGG - chr6_3:5429270-5429289 Msa0935060:intron 50.0%
GAGGAAACAACAGCTAGAGG+AGG - chr6_3:5429273-5429292 Msa0935060:intron 50.0%
AACAACCCACCCGATCGTGT+CGG - chr6_3:5425788-5425807 Msa0935060:three_prime_UTR 55.0%
CGAATGGGTGCAACAGGATC+AGG - chr6_3:5427020-5427039 Msa0935060:intron 55.0%
GTGCAACAGGATCAGGGGTA+TGG - chr6_3:5427027-5427046 Msa0935060:intron 55.0%
! CCAAGGGAGCTGCCACATTT+TGG + chr6_3:5428610-5428629 None:intergenic 55.0%
CCAAAATGTGGCAGCTCCCT+TGG - chr6_3:5428607-5428626 Msa0935060:intron 55.0%
TCAGAAGGAGGCTCCTCAAC+TGG + chr6_3:5428642-5428661 None:intergenic 55.0%
AGTTGAGGAGCCTCCTTCTG+AGG - chr6_3:5428641-5428660 Msa0935060:intron 55.0%
GAGAAATGGCTGAGGCGAAG+AGG - chr6_3:5428720-5428739 Msa0935060:intron 55.0%
! GACTGGCTATGTCTCTAGGC+AGG - chr6_3:5428847-5428866 Msa0935060:intron 55.0%
TTCTCAGTAGACGCAGCGTC+AGG + chr6_3:5428947-5428966 None:intergenic 55.0%
CTGCCGTCAGCATGTCATCA+GGG + chr6_3:5428988-5429007 None:intergenic 55.0%
TCTGCCGTCAGCATGTCATC+AGG + chr6_3:5428989-5429008 None:intergenic 55.0%
ACAACCCACCCGATCGTGTC+GGG - chr6_3:5425789-5425808 Msa0935060:three_prime_UTR 60.0%
CAACTGGAGGTGGCACCCAA+GGG + chr6_3:5428626-5428645 None:intergenic 60.0%
TCAACTGGAGGTGGCACCCA+AGG + chr6_3:5428627-5428646 None:intergenic 60.0%
! TTGGGTGCCACCTCCAGTTG+AGG - chr6_3:5428626-5428645 Msa0935060:intron 60.0%
GAAGGAGGCTCCTCAACTGG+AGG + chr6_3:5428639-5428658 None:intergenic 60.0%
TGACATGCTGACGGCAGAGC+AGG - chr6_3:5428991-5429010 Msa0935060:intron 60.0%
ATGCTGACGGCAGAGCAGGT+GGG - chr6_3:5428995-5429014 Msa0935060:intron 60.0%
CAACCCACCCGATCGTGTCG+GGG - chr6_3:5425790-5425809 Msa0935060:three_prime_UTR 65.0%
ACGGTGAGCCCCGACACGAT+CGG + chr6_3:5425801-5425820 None:intergenic 65.0%
CGGGCGCGACATAAAGCCAC+AGG - chr6_3:5426052-5426071 Msa0935060:three_prime_UTR 65.0%
GGAGGCTCCTCAACTGGAGG+TGG + chr6_3:5428636-5428655 None:intergenic 65.0%
CATGCTGACGGCAGAGCAGG+TGG - chr6_3:5428994-5429013 Msa0935060:intron 65.0%
GAGCCCCGACACGATCGGGT+GGG + chr6_3:5425796-5425815 None:intergenic 70.0%
TGAGCCCCGACACGATCGGG+TGG + chr6_3:5425797-5425816 None:intergenic 70.0%
CGGTGAGCCCCGACACGATC+GGG + chr6_3:5425800-5425819 None:intergenic 70.0%
GTGTCGGGGCTCACCGTGCT+GGG - chr6_3:5425804-5425823 Msa0935060:three_prime_UTR 70.0%
TGTCGGGGCTCACCGTGCTG+GGG - chr6_3:5425805-5425824 Msa0935060:three_prime_UTR 70.0%
!! AGGAAGCGCCAGCCCCAGCA+CGG + chr6_3:5425820-5425839 None:intergenic 70.0%
CGTGTCGGGGCTCACCGTGC+TGG - chr6_3:5425803-5425822 Msa0935060:three_prime_UTR 75.0%
! GGGGCTCACCGTGCTGGGGC+TGG - chr6_3:5425809-5425828 Msa0935060:three_prime_UTR 80.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 5425725 5429627 5425725 ID=Msa0935060;Name=Msa0935060
chr6_3 mRNA 5425725 5429627 5425725 ID=Msa0935060-mRNA-1;Parent=Msa0935060;Name=Msa0935060-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0.71|0.75|1|0.85|0.75|8|738|226
chr6_3 exon 5425725 5426145 5425725 ID=Msa0935060-mRNA-1:exon:1658;Parent=Msa0935060-mRNA-1
chr6_3 exon 5426340 5426773 5426340 ID=Msa0935060-mRNA-1:exon:1657;Parent=Msa0935060-mRNA-1
chr6_3 exon 5426859 5426943 5426859 ID=Msa0935060-mRNA-1:exon:1656;Parent=Msa0935060-mRNA-1
chr6_3 exon 5427033 5427142 5427033 ID=Msa0935060-mRNA-1:exon:1655;Parent=Msa0935060-mRNA-1
chr6_3 exon 5428309 5428353 5428309 ID=Msa0935060-mRNA-1:exon:1654;Parent=Msa0935060-mRNA-1
chr6_3 exon 5429001 5429125 5429001 ID=Msa0935060-mRNA-1:exon:1653;Parent=Msa0935060-mRNA-1
chr6_3 exon 5429279 5429349 5429279 ID=Msa0935060-mRNA-1:exon:1652;Parent=Msa0935060-mRNA-1
chr6_3 exon 5429500 5429627 5429500 ID=Msa0935060-mRNA-1:exon:1651;Parent=Msa0935060-mRNA-1
chr6_3 CDS 5429500 5429627 5429500 ID=Msa0935060-mRNA-1:cds;Parent=Msa0935060-mRNA-1
chr6_3 CDS 5429279 5429349 5429279 ID=Msa0935060-mRNA-1:cds;Parent=Msa0935060-mRNA-1
chr6_3 CDS 5429001 5429125 5429001 ID=Msa0935060-mRNA-1:cds;Parent=Msa0935060-mRNA-1
chr6_3 CDS 5428309 5428353 5428309 ID=Msa0935060-mRNA-1:cds;Parent=Msa0935060-mRNA-1
chr6_3 CDS 5427033 5427142 5427033 ID=Msa0935060-mRNA-1:cds;Parent=Msa0935060-mRNA-1
chr6_3 CDS 5426859 5426943 5426859 ID=Msa0935060-mRNA-1:cds;Parent=Msa0935060-mRNA-1
chr6_3 CDS 5426657 5426773 5426657 ID=Msa0935060-mRNA-1:cds;Parent=Msa0935060-mRNA-1
chr6_3 three_prime_UTR 5426340 5426656 5426340 ID=Msa0935060-mRNA-1:three_prime_utr;Parent=Msa0935060-mRNA-1
chr6_3 three_prime_UTR 5425725 5426145 5425725 ID=Msa0935060-mRNA-1:three_prime_utr;Parent=Msa0935060-mRNA-1
Gene Sequence

>Msa0935060

ATGTCCCTACAAAGTACAAACCGATTTAACTTTGTCAACAAAAAAGGAACATTAGTTGACTTCAACAACCCACCCGATCGTGTCGGGGCTCACCGTGCTGGGGCTGGCGCTTCCTATTTCCGTATTGGATGGGCTGGTGAGTCTGAACCTCGTGTTGTTTTTCGCAACATTGTTCAACGGGCGCGACATAAAGCCACAGGAGAAACTGTTACCATTGTTGGTGACCATGATCCAGCATTGTTGAAGTACTTTGATTGTACTCGTTCTGGACCACGATCAGCATTTGAAAGCGATGTTGTTTTTCAGTTTGAAATAAAGGAATATATTCTGGACTTTGCTTTTGATCGAATGGGTGCAACAGGATCAGGGTTTGTTGACGGGGAACCTATTTATAAAGGAAGCTGTCGCACTAATATTGGTGGATTCCATGTGACTGATTATTTTCAGCGACTTCTTTCACTTAAATATCCTTATCATACGGCTAGATTTACATGGGAAAAGGTTGAAGACCTGAAGATGGAAAGATTGTTATGTTGCACAGGACTATGTTTCAGAAGCTCGATTAAAGGAGCTAAGGAGGCCAAAGGAAAAACCAAAATGTGGCAGCTCCCTTGGGTGCCACCTCCAGTTGAGGAGCCTCCTTCTGAGGAAGAGATTGCAAGAAAAGAAAGGCAGCAATAA
Protein sequence

>Msa0935060

MSLQSTNRFNFVNKKGTLVDFNNPPDRVGAHRAGAGASYFRIGWAGESEPRVVFRNIVQRARHKATGETVTIVGDHDPALLKYFDCTRSGPRSAFESDVVFQFEIKEYILDFAFDRMGATGSGFVDGEPIYKGSCRTNIGGFHVTDYFQRLLSLKYPYHTARFTWEKVEDLKMERLLCCTGLCFRSSIKGAKEAKGKTKMWQLPWVPPPVEEPPSEEEIARKERQQ*