Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0937250 | sp|Q9LJW5|MIF2_ARATH | 59.596 | 99 | 25 | 3 | 1 | 84 | 1 | 99 | 5.99e-30 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0937250 | G7KKH9 | 94.118 | 85 | 1 | 1 | 1 | 85 | 1 | 81 | 1.38e-49 | 160 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0937250 | TF | zf-HD |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0937250 | Msa0974550 | 0.907009 | 8.043537e-81 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0937250 | MtrunA17_Chr6g0453221 | 92.941 | 85 | 2 | 1 | 1 | 85 | 1 | 81 | 1.50e-52 | 158 |
| Msa0937250 | MtrunA17_Chr3g0092371 | 68.675 | 83 | 23 | 2 | 1 | 81 | 1 | 82 | 9.29e-36 | 116 |
| Msa0937250 | MtrunA17_Chr1g0188441 | 58.209 | 67 | 28 | 0 | 15 | 81 | 3 | 69 | 8.83e-23 | 86.3 |
| Msa0937250 | MtrunA17_Chr4g0060731 | 65.333 | 75 | 19 | 3 | 1 | 71 | 1 | 72 | 1.36e-22 | 82.8 |
| Msa0937250 | MtrunA17_Chr3g0133181 | 60.000 | 55 | 22 | 0 | 18 | 72 | 77 | 131 | 3.18e-20 | 81.6 |
| Msa0937250 | MtrunA17_Chr2g0315941 | 56.923 | 65 | 26 | 1 | 21 | 83 | 68 | 132 | 4.23e-20 | 80.9 |
| Msa0937250 | MtrunA17_Chr8g0367691 | 56.061 | 66 | 27 | 1 | 20 | 83 | 68 | 133 | 6.56e-20 | 80.5 |
| Msa0937250 | MtrunA17_Chr4g0076821 | 63.158 | 57 | 21 | 0 | 20 | 76 | 112 | 168 | 3.35e-19 | 79.7 |
| Msa0937250 | MtrunA17_Chr8g0361671 | 65.455 | 55 | 17 | 1 | 18 | 70 | 61 | 115 | 1.24e-18 | 78.2 |
| Msa0937250 | MtrunA17_Chr7g0217231 | 57.627 | 59 | 23 | 1 | 16 | 72 | 83 | 141 | 1.68e-18 | 77.4 |
| Msa0937250 | MtrunA17_Chr5g0427051 | 56.667 | 60 | 26 | 0 | 19 | 78 | 56 | 115 | 2.70e-18 | 76.3 |
| Msa0937250 | MtrunA17_Chr5g0412501 | 52.459 | 61 | 29 | 0 | 20 | 80 | 77 | 137 | 4.73e-18 | 75.5 |
| Msa0937250 | MtrunA17_Chr5g0404271 | 59.615 | 52 | 21 | 0 | 21 | 72 | 99 | 150 | 5.03e-18 | 75.9 |
| Msa0937250 | MtrunA17_Chr6g0453231 | 59.259 | 54 | 20 | 1 | 21 | 72 | 91 | 144 | 3.82e-16 | 70.9 |
| Msa0937250 | MtrunA17_Chr6g0479901 | 50.000 | 60 | 29 | 1 | 21 | 79 | 19 | 78 | 9.72e-15 | 65.5 |
| Msa0937250 | MtrunA17_Chr8g0357721 | 50.000 | 38 | 19 | 0 | 30 | 67 | 14 | 51 | 1.61e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0937250 | AT3G28917.1 | 59.596 | 99 | 25 | 3 | 1 | 84 | 1 | 99 | 6.09e-31 | 104 |
| Msa0937250 | AT1G18835.1 | 60.000 | 90 | 26 | 3 | 1 | 83 | 1 | 87 | 2.03e-29 | 100 |
| Msa0937250 | AT1G74660.1 | 50.495 | 101 | 31 | 2 | 1 | 82 | 2 | 102 | 3.56e-27 | 95.5 |
| Msa0937250 | AT1G74660.2 | 50.562 | 89 | 27 | 1 | 1 | 72 | 2 | 90 | 2.14e-25 | 90.9 |
| Msa0937250 | AT4G24660.1 | 66.071 | 56 | 19 | 0 | 19 | 74 | 47 | 102 | 1.15e-22 | 87.0 |
| Msa0937250 | AT4G24660.2 | 66.071 | 56 | 19 | 0 | 19 | 74 | 47 | 102 | 1.34e-22 | 87.0 |
| Msa0937250 | AT2G02540.2 | 55.224 | 67 | 30 | 0 | 6 | 72 | 72 | 138 | 5.63e-22 | 87.0 |
| Msa0937250 | AT2G02540.1 | 55.224 | 67 | 30 | 0 | 6 | 72 | 72 | 138 | 5.63e-22 | 87.0 |
| Msa0937250 | AT1G14440.1 | 55.072 | 69 | 29 | 1 | 15 | 81 | 84 | 152 | 1.92e-21 | 85.5 |
| Msa0937250 | AT1G14440.2 | 55.072 | 69 | 29 | 1 | 15 | 81 | 84 | 152 | 1.92e-21 | 85.5 |
| Msa0937250 | AT5G15210.1 | 59.375 | 64 | 24 | 1 | 17 | 78 | 52 | 115 | 6.54e-20 | 80.9 |
| Msa0937250 | AT3G50890.1 | 54.412 | 68 | 28 | 1 | 7 | 71 | 43 | 110 | 8.13e-20 | 80.1 |
| Msa0937250 | AT1G75240.1 | 59.677 | 62 | 25 | 0 | 16 | 77 | 71 | 132 | 1.01e-19 | 80.9 |
| Msa0937250 | AT5G65410.1 | 60.000 | 55 | 22 | 0 | 17 | 71 | 71 | 125 | 4.51e-19 | 79.0 |
| Msa0937250 | AT2G18350.1 | 56.716 | 67 | 26 | 1 | 19 | 82 | 80 | 146 | 6.36e-19 | 78.2 |
| Msa0937250 | AT1G69600.1 | 63.462 | 52 | 17 | 1 | 21 | 70 | 31 | 82 | 1.62e-17 | 73.9 |
| Msa0937250 | AT3G28920.1 | 57.812 | 64 | 25 | 1 | 19 | 80 | 50 | 113 | 9.26e-16 | 70.1 |
| Msa0937250 | AT5G60480.1 | 57.407 | 54 | 21 | 1 | 18 | 69 | 34 | 87 | 3.33e-15 | 67.8 |
| Msa0937250 | AT5G39760.1 | 48.000 | 75 | 37 | 1 | 6 | 78 | 41 | 115 | 9.18e-15 | 67.8 |
| Msa0937250 | AT5G42780.1 | 58.824 | 51 | 19 | 1 | 21 | 71 | 64 | 112 | 1.51e-14 | 66.2 |
| Msa0937250 | AT1G14687.1 | 50.769 | 65 | 23 | 4 | 21 | 85 | 7 | 62 | 2.97e-14 | 64.3 |
Find 11 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAGAAGTGTTGTGAAATA+TGG | 0.276912 | 6_3:+8417200 | Msa0937250:CDS |
| GTTGGAGGTTATGCTGTTGA+TGG | 0.403038 | 6_3:+8417251 | Msa0937250:CDS |
| TTTGTACCTTGTTCACTACT+TGG | 0.415225 | 6_3:-8417290 | None:intergenic |
| TATTTCACAACACTTCTTTG+TGG | 0.421230 | 6_3:-8417197 | None:intergenic |
| AAGAATCATGCAGCTAATGT+TGG | 0.502606 | 6_3:+8417233 | Msa0937250:CDS |
| AGTAGTGAACAAGGTACAAA+TGG | 0.504202 | 6_3:+8417293 | Msa0937250:CDS |
| AGTTCTTCACCTCACTCAAA+TGG | 0.507969 | 6_3:+8417386 | Msa0937250:CDS |
| TGTCTCACTTGTGCTGCTTG+TGG | 0.516521 | 6_3:+8417317 | Msa0937250:CDS |
| AATCATGCAGCTAATGTTGG+AGG | 0.567933 | 6_3:+8417236 | Msa0937250:CDS |
| TTTCAAGTACCATTTGAGTG+AGG | 0.635538 | 6_3:-8417395 | None:intergenic |
| TTCATGCCAAGTAGTGAACA+AGG | 0.658987 | 6_3:+8417284 | Msa0937250:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTTCACAACACTTCTTTG+TGG | - | chr6_3:8417200-8417219 | None:intergenic | 30.0% | |
| !! | CAAAGAAGTGTTGTGAAATA+TGG | + | chr6_3:8417200-8417219 | Msa0937250:CDS | 30.0% |
| AAGAATCATGCAGCTAATGT+TGG | + | chr6_3:8417233-8417252 | Msa0937250:CDS | 35.0% | |
| TTTGTACCTTGTTCACTACT+TGG | - | chr6_3:8417293-8417312 | None:intergenic | 35.0% | |
| AGTAGTGAACAAGGTACAAA+TGG | + | chr6_3:8417293-8417312 | Msa0937250:CDS | 35.0% | |
| AATCATGCAGCTAATGTTGG+AGG | + | chr6_3:8417236-8417255 | Msa0937250:CDS | 40.0% | |
| TTCATGCCAAGTAGTGAACA+AGG | + | chr6_3:8417284-8417303 | Msa0937250:CDS | 40.0% | |
| AGTTCTTCACCTCACTCAAA+TGG | + | chr6_3:8417386-8417405 | Msa0937250:CDS | 40.0% | |
| GTTGGAGGTTATGCTGTTGA+TGG | + | chr6_3:8417251-8417270 | Msa0937250:CDS | 45.0% | |
| !! | TGTCTCACTTGTGCTGCTTG+TGG | + | chr6_3:8417317-8417336 | Msa0937250:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 8417158 | 8417415 | 8417158 | ID=Msa0937250;Name=Msa0937250 |
| chr6_3 | mRNA | 8417158 | 8417415 | 8417158 | ID=Msa0937250-mRNA-1;Parent=Msa0937250;Name=Msa0937250-mRNA-1;_AED=0.27;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|85 |
| chr6_3 | exon | 8417158 | 8417415 | 8417158 | ID=Msa0937250-mRNA-1:exon:2740;Parent=Msa0937250-mRNA-1 |
| chr6_3 | CDS | 8417158 | 8417415 | 8417158 | ID=Msa0937250-mRNA-1:cds;Parent=Msa0937250-mRNA-1 |
| Gene Sequence |
| Protein sequence |
