AlfalfaGEDB | Msa0974550

M. sativa cultivar zhongmu-4 / Msa0974550

KEGG: -
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009908 GO:0010022 GO:0010073 GO:0010582 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046983 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048507 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0097159 GO:0099402 GO:1901363
PFAM: PF04770.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0974550	XP_039682709.1	94.118	85	1	1	1	85	1	81	2.88e-49	160

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0974550	sp\|Q9LJW5\|MIF2_ARATH	59.596	99	25	3	1	84	1	99	5.99e-30	104

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0974550	G7KKH9	94.118	85	1	1	1	85	1	81	1.38e-49	160

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TFs/TRs:

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Gene ID	Type	Classification
Msa0974550	TF	zf-HD

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0937250	Msa0974550	0.907009	8.043537e-81	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0974550	MtrunA17_Chr6g0453221	92.941	85	2	1	1	85	1	81	1.50e-52	158
Msa0974550	MtrunA17_Chr3g0092371	68.675	83	23	2	1	81	1	82	9.29e-36	116
Msa0974550	MtrunA17_Chr1g0188441	58.209	67	28	0	15	81	3	69	8.83e-23	86.3
Msa0974550	MtrunA17_Chr4g0060731	65.333	75	19	3	1	71	1	72	1.36e-22	82.8
Msa0974550	MtrunA17_Chr3g0133181	60.000	55	22	0	18	72	77	131	3.18e-20	81.6
Msa0974550	MtrunA17_Chr2g0315941	56.923	65	26	1	21	83	68	132	4.23e-20	80.9
Msa0974550	MtrunA17_Chr8g0367691	56.061	66	27	1	20	83	68	133	6.56e-20	80.5
Msa0974550	MtrunA17_Chr4g0076821	63.158	57	21	0	20	76	112	168	3.35e-19	79.7
Msa0974550	MtrunA17_Chr8g0361671	65.455	55	17	1	18	70	61	115	1.24e-18	78.2
Msa0974550	MtrunA17_Chr7g0217231	57.627	59	23	1	16	72	83	141	1.68e-18	77.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0974550	AT3G28917.1	59.596	99	25	3	1	84	1	99	6.09e-31	104
Msa0974550	AT1G18835.1	60.000	90	26	3	1	83	1	87	2.03e-29	100
Msa0974550	AT1G74660.1	50.495	101	31	2	1	82	2	102	3.56e-27	95.5
Msa0974550	AT1G74660.2	50.562	89	27	1	1	72	2	90	2.14e-25	90.9
Msa0974550	AT4G24660.1	66.071	56	19	0	19	74	47	102	1.15e-22	87.0
Msa0974550	AT4G24660.2	66.071	56	19	0	19	74	47	102	1.34e-22	87.0
Msa0974550	AT2G02540.2	55.224	67	30	0	6	72	72	138	5.63e-22	87.0
Msa0974550	AT2G02540.1	55.224	67	30	0	6	72	72	138	5.63e-22	87.0
Msa0974550	AT1G14440.1	55.072	69	29	1	15	81	84	152	1.92e-21	85.5
Msa0974550	AT1G14440.2	55.072	69	29	1	15	81	84	152	1.92e-21	85.5

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Find 11 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAATCATGCAGCTAATGT+TGG	0.502606	6_4:+7643931	Msa0974550:CDS
AATCATGCAGCTAATGTTGG+AGG	0.567933	6_4:+7643934	Msa0974550:CDS
AGTAGTGAACAAGGTACAAA+TGG	0.504202	6_4:+7643991	Msa0974550:CDS
AGTTCTTCACCTCACTCAAA+TGG	0.507969	6_4:+7644084	Msa0974550:CDS
CAAAGAAGTGTTGTGAAATA+TGG	0.276912	6_4:+7643898	Msa0974550:CDS
GTTGGAGGTTATGCTGTTGA+TGG	0.403038	6_4:+7643949	Msa0974550:CDS
TATTTCACAACACTTCTTTG+TGG	0.421230	6_4:-7643895	None:intergenic
TGTCTCACTTGTGCTGCTTG+TGG	0.516521	6_4:+7644015	Msa0974550:CDS
TTCATGCCAAGTAGTGAACA+AGG	0.658987	6_4:+7643982	Msa0974550:CDS
TTTCAAGTACCATTTGAGTG+AGG	0.635538	6_4:-7644093	None:intergenic

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TATTTCACAACACTTCTTTG+TGG	-	chr6_4:7643898-7643917	None:intergenic	30.0%
	AAGAATCATGCAGCTAATGT+TGG	+	chr6_4:7643931-7643950	Msa0974550:CDS	35.0%
	TTTGTACCTTGTTCACTACT+TGG	-	chr6_4:7643991-7644010	None:intergenic	35.0%
	AGTAGTGAACAAGGTACAAA+TGG	+	chr6_4:7643991-7644010	Msa0974550:CDS	35.0%
	AATCATGCAGCTAATGTTGG+AGG	+	chr6_4:7643934-7643953	Msa0974550:CDS	40.0%
	TTCATGCCAAGTAGTGAACA+AGG	+	chr6_4:7643982-7644001	Msa0974550:CDS	40.0%
	AGTTCTTCACCTCACTCAAA+TGG	+	chr6_4:7644084-7644103	Msa0974550:CDS	40.0%
	GTTGGAGGTTATGCTGTTGA+TGG	+	chr6_4:7643949-7643968	Msa0974550:CDS	45.0%
!!	CAAAGAAGTGTTGTGAAATA+TGG	+	chr6_4:7643898-7643917	Msa0974550:CDS	30.0%
!!	TGTCTCACTTGTGCTGCTTG+TGG	+	chr6_4:7644015-7644034	Msa0974550:CDS	50.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	7643856	7644113	7643856	ID=Msa0974550;Name=Msa0974550
chr6_4	mRNA	7643856	7644113	7643856	ID=Msa0974550-mRNA-1;Parent=Msa0974550;Name=Msa0974550-mRNA-1;_AED=0.27;_eAED=0.28;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|85
chr6_4	exon	7643856	7644113	7643856	ID=Msa0974550-mRNA-1:exon:2937;Parent=Msa0974550-mRNA-1
chr6_4	CDS	7643856	7644113	7643856	ID=Msa0974550-mRNA-1:cds;Parent=Msa0974550-mRNA-1

Gene Sequence	>Msa0974550 ATGAGGAAGAGACAAGTAGTTGTGAGAAGATCAGAAGATCCACAAAGAAGTGTTGTGAAATATGGAGAGTGTCAAAAGAATCATGCAGCTAATGTTGGAGGTTATGCTGTTGATGGTTGTAGAGAGTTCATGCCAAGTAGTGAACAAGGTACAAATGGTTGTCTCACTTGTGCTGCTTGTGGTTGTCACAGAAACTTTCACAAAAGAGAAGTTGAAGTTGTTTCTGAGAGTTCTTCACCTCACTCAAATGGTACTTGA
Protein sequence	>Msa0974550 MRKRQVVVRRSEDPQRSVVKYGECQKNHAANVGGYAVDGCREFMPSSEQGTNGCLTCAACGCHRNFHKREVEVVSESSSPHSNGT*