AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0938180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0938180 MtrunA17_Chr6g0454071 93.007 143 10 0 19 161 813 955 3.07e-87 275
Msa0938180 MtrunA17_Chr7g0218421 81.875 160 29 0 19 178 817 976 2.52e-84 267
Msa0938180 MtrunA17_Chr3g0137071 55.474 137 61 0 27 163 834 970 3.90e-46 161
Msa0938180 MtrunA17_Chr3g0131351 38.806 134 77 2 17 150 820 948 7.30e-24 97.8
Msa0938180 MtrunA17_Chr4g0061371 36.364 132 83 1 11 141 722 853 2.15e-23 96.3
Msa0938180 MtrunA17_Chr7g0225161 32.857 140 93 1 2 141 714 852 6.44e-22 92.0
Msa0938180 MtrunA17_Chr3g0131711 36.765 136 83 1 23 155 779 914 1.76e-21 90.9
Msa0938180 MtrunA17_Chr3g0134631 37.692 130 78 1 23 149 784 913 4.06e-21 89.7
Msa0938180 MtrunA17_Chr8g0385811 37.405 131 79 1 23 150 783 913 4.10e-21 89.7
Msa0938180 MtrunA17_Chr3g0102771 38.053 113 70 0 31 143 663 775 1.36e-18 82.4
Msa0938180 MtrunA17_Chr2g0277771 30.827 133 86 2 11 142 3 130 1.55e-18 78.6
Msa0938180 MtrunA17_Chr2g0329561 25.735 136 101 0 5 140 312 447 2.20e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0938180 AT5G15450.1 71.698 159 41 2 11 165 798 956 1.24e-72 236
Msa0938180 AT2G25140.1 53.020 149 64 2 19 167 817 959 1.50e-44 157
Msa0938180 AT5G51070.1 38.596 114 70 0 26 139 804 917 3.41e-24 99.0
Msa0938180 AT1G74310.1 33.571 140 93 0 2 141 711 850 3.27e-22 93.2
Msa0938180 AT5G50920.1 38.168 131 77 1 23 149 781 911 7.22e-22 92.4
Msa0938180 AT3G48870.2 37.500 120 75 0 23 142 771 890 2.06e-20 88.2
Msa0938180 AT3G48870.1 37.500 120 75 0 23 142 802 921 2.10e-20 88.2
Msa0938180 AT3G48870.4 37.500 120 75 0 23 142 802 921 2.10e-20 88.2
Msa0938180 AT3G48870.3 37.500 120 75 0 23 142 802 921 2.10e-20 88.2
Msa0938180 AT1G74310.2 31.373 102 70 0 2 103 711 812 2.69e-11 61.6

Find 45 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTCACAATGCTAATTATT+TGG 0.181666 6_3:+9896877 None:intergenic
TCATTTCAAATACTATTTCT+AGG 0.203814 6_3:+9896060 None:intergenic
ATCCAACTTCTTGGAAGTTT+AGG 0.235052 6_3:-9896532 Msa0938180:CDS
TATTCTTCGAGGAGAATTTA+AGG 0.278524 6_3:-9896434 Msa0938180:CDS
AATTAGCATTGTGAGGTTAC+AGG 0.286083 6_3:-9896871 Msa0938180:intron
ATCTATTACACGCTGCTTCA+TGG 0.290601 6_3:+9897064 None:intergenic
TCCAACTTCTTGGAAGTTTA+GGG 0.290774 6_3:-9896531 Msa0938180:CDS
GAACCTGCTATCCAACTTCT+TGG 0.317322 6_3:-9896541 Msa0938180:CDS
AACTTGGCATGGAAGATAAA+AGG 0.330056 6_3:+9896248 None:intergenic
ATCGTTCTGTTATTATAGAT+TGG 0.336034 6_3:-9896205 Msa0938180:three_prime_UTR
ATCCATTACACGCTGCTTCA+TGG 0.342692 6_3:+9896977 None:intergenic
GTTTCATATGCCAAATCTTT+CGG 0.360088 6_3:+9896999 None:intergenic
GAATAGTGCACCGAAAGATT+TGG 0.388532 6_3:-9897009 Msa0938180:CDS
ACCCTAAACTTCCAAGAAGT+TGG 0.420686 6_3:+9896530 None:intergenic
GTTATTATAGATTGGAACTC+TGG 0.429182 6_3:-9896197 Msa0938180:three_prime_UTR
GATGCTGCAAGATCCATCTT+TGG 0.436829 6_3:-9897044 Msa0938180:CDS
TCTCAAAATTCGTGTAAACT+TGG 0.442353 6_3:+9896232 None:intergenic
CTTCCAAGAAGTTGGATAGC+AGG 0.453391 6_3:+9896538 None:intergenic
ATCAACTCTGTTCATGAACT+CGG 0.481373 6_3:+9896932 None:intergenic
CATGAACTCAGGGCCAAAGA+TGG 0.482412 6_3:+9897031 None:intergenic
TCGAGGAGAATTTAAGGAAG+AGG 0.483569 6_3:-9896428 Msa0938180:CDS
TGTTGAATCACTCGCTTCAC+CGG 0.489454 6_3:+9896484 None:intergenic
GGATAGCAGGTTCTGTTACT+CGG 0.501544 6_3:+9896551 None:intergenic
TCAACTCTGTTCATGAACTC+GGG 0.501732 6_3:+9896933 None:intergenic
ACCGGTCTAGCACCATAGCT+TGG 0.505665 6_3:+9896502 None:intergenic
TTGCTGAGGACGTTGACCAT+TGG 0.509376 6_3:+9896369 None:intergenic
AATCTACTGCTAAAGACAGC+AGG 0.516797 6_3:-9896312 Msa0938180:CDS
GGTGCACTATTCATGAACTC+AGG 0.517076 6_3:+9897020 None:intergenic
AAATTTCTTCATAGAATCTG+TGG 0.517735 6_3:+9896280 None:intergenic
CTTGCAAAGGGTATTCTTCG+AGG 0.524988 6_3:-9896445 Msa0938180:CDS
AACCATGAAGCAGCGTGTAA+TGG 0.525366 6_3:-9896979 Msa0938180:CDS
TCCAAGCTATGGTGCTAGAC+CGG 0.530798 6_3:-9896503 Msa0938180:CDS
GTGGAAAATGAACTTGCAAA+GGG 0.543223 6_3:-9896457 Msa0938180:CDS
TGTGGAAAATGAACTTGCAA+AGG 0.545576 6_3:-9896458 Msa0938180:CDS
CAATGCTAATTATTTGGTGA+CGG 0.561942 6_3:+9896883 None:intergenic
GCGAGTGATTCAACAAAATG+TGG 0.563340 6_3:-9896476 Msa0938180:CDS
ACCAAATAATTAGCATTGTG+AGG 0.566209 6_3:-9896878 Msa0938180:CDS
AAATTCGTGTAAACTTGGCA+TGG 0.589380 6_3:+9896237 None:intergenic
TTAGGGTACGATCCAAGCTA+TGG 0.593819 6_3:-9896514 Msa0938180:CDS
ATCTACTGCTAAAGACAGCA+GGG 0.595696 6_3:-9896311 Msa0938180:CDS
ATTATTTGGTGACGGTCAAG+AGG 0.598093 6_3:+9896891 None:intergenic
CAACTCTGTTCATGAACTCG+GGG 0.614737 6_3:+9896934 None:intergenic
GTGCACTATTCATGAACTCA+GGG 0.621416 6_3:+9897021 None:intergenic
GAAGTCACAATACTCGCCAA+TGG 0.657171 6_3:-9896385 Msa0938180:CDS
AGTATATGCAAGCTATACAC+AGG 0.666070 6_3:+9896173 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGAAATATAAATTAGAA+TGG + chr6_3:9896260-9896279 None:intergenic 10.0%
!!! GTTTATATTTTACTTTATAT+TGG - chr6_3:9896940-9896959 Msa0938180:CDS 10.0%
!! AAATGAAATATAAATTAGAA+TGG + chr6_3:9896260-9896279 None:intergenic 10.0%
!!! GTTTATATTTTACTTTATAT+TGG - chr6_3:9896940-9896959 Msa0938180:CDS 10.0%
!!! TTGATGAGTATATTGTTTTT+CGG - chr6_3:9896168-9896187 Msa0938180:three_prime_UTR 20.0%
!!! TTTATGTTTGTTATTACAGT+TGG - chr6_3:9896468-9896487 Msa0938180:CDS 20.0%
!!! TCATTTCAAATACTATTTCT+AGG + chr6_3:9897025-9897044 None:intergenic 20.0%
!!! TTGATGAGTATATTGTTTTT+CGG - chr6_3:9896168-9896187 Msa0938180:three_prime_UTR 20.0%
!!! TTTATGTTTGTTATTACAGT+TGG - chr6_3:9896468-9896487 Msa0938180:CDS 20.0%
!!! TCATTTCAAATACTATTTCT+AGG + chr6_3:9897025-9897044 None:intergenic 20.0%
! CAAAGAACAAAGTATACTAT+AGG + chr6_3:9896303-9896322 None:intergenic 25.0%
! CAATCAGTATTTGTATGATA+AGG - chr6_3:9896354-9896373 Msa0938180:CDS 25.0%
! AAATTTCTTCATAGAATCTG+TGG + chr6_3:9896805-9896824 None:intergenic 25.0%
! ATCGTTCTGTTATTATAGAT+TGG - chr6_3:9896877-9896896 Msa0938180:CDS 25.0%
! CAAAGAACAAAGTATACTAT+AGG + chr6_3:9896303-9896322 None:intergenic 25.0%
! CAATCAGTATTTGTATGATA+AGG - chr6_3:9896354-9896373 Msa0938180:CDS 25.0%
! AAATTTCTTCATAGAATCTG+TGG + chr6_3:9896805-9896824 None:intergenic 25.0%
! ATCGTTCTGTTATTATAGAT+TGG - chr6_3:9896877-9896896 Msa0938180:CDS 25.0%
GTTTCATATGCCAAATCTTT+CGG + chr6_3:9896086-9896105 None:intergenic 30.0%
! CAATGCTAATTATTTGGTGA+CGG + chr6_3:9896202-9896221 None:intergenic 30.0%
ACCAAATAATTAGCATTGTG+AGG - chr6_3:9896204-9896223 Msa0938180:three_prime_UTR 30.0%
ACCTCACAATGCTAATTATT+TGG + chr6_3:9896208-9896227 None:intergenic 30.0%
CTATAGGAACTCAAAAAAAG+AGG + chr6_3:9896287-9896306 None:intergenic 30.0%
TATTCTTCGAGGAGAATTTA+AGG - chr6_3:9896648-9896667 Msa0938180:intron 30.0%
TCTCAAAATTCGTGTAAACT+TGG + chr6_3:9896853-9896872 None:intergenic 30.0%
GTTATTATAGATTGGAACTC+TGG - chr6_3:9896885-9896904 Msa0938180:CDS 30.0%
GTTTCATATGCCAAATCTTT+CGG + chr6_3:9896086-9896105 None:intergenic 30.0%
! CAATGCTAATTATTTGGTGA+CGG + chr6_3:9896202-9896221 None:intergenic 30.0%
ACCAAATAATTAGCATTGTG+AGG - chr6_3:9896204-9896223 Msa0938180:three_prime_UTR 30.0%
ACCTCACAATGCTAATTATT+TGG + chr6_3:9896208-9896227 None:intergenic 30.0%
CTATAGGAACTCAAAAAAAG+AGG + chr6_3:9896287-9896306 None:intergenic 30.0%
TATTCTTCGAGGAGAATTTA+AGG - chr6_3:9896648-9896667 Msa0938180:intron 30.0%
TCTCAAAATTCGTGTAAACT+TGG + chr6_3:9896853-9896872 None:intergenic 30.0%
GTTATTATAGATTGGAACTC+TGG - chr6_3:9896885-9896904 Msa0938180:CDS 30.0%
ATCAACTCTGTTCATGAACT+CGG + chr6_3:9896153-9896172 None:intergenic 35.0%
AATTAGCATTGTGAGGTTAC+AGG - chr6_3:9896211-9896230 Msa0938180:three_prime_UTR 35.0%
TTAGAATGGATCGATTAGTG+AGG + chr6_3:9896246-9896265 None:intergenic 35.0%
! TTACTCGGATTTTCATCTTG+CGG + chr6_3:9896519-9896538 None:intergenic 35.0%
ATCCAACTTCTTGGAAGTTT+AGG - chr6_3:9896550-9896569 Msa0938180:CDS 35.0%
TCCAACTTCTTGGAAGTTTA+GGG - chr6_3:9896551-9896570 Msa0938180:CDS 35.0%
TGTGGAAAATGAACTTGCAA+AGG - chr6_3:9896624-9896643 Msa0938180:intron 35.0%
GTGGAAAATGAACTTGCAAA+GGG - chr6_3:9896625-9896644 Msa0938180:intron 35.0%
!!! CTAAAAACTAGCTTTTGCTG+AGG + chr6_3:9896730-9896749 None:intergenic 35.0%
!!! CTCAGCAAAAGCTAGTTTTT+AGG - chr6_3:9896728-9896747 Msa0938180:intron 35.0%
!!! AGCAAAAGCTAGTTTTTAGG+AGG - chr6_3:9896731-9896750 Msa0938180:intron 35.0%
!!! GCAAAAGCTAGTTTTTAGGA+GGG - chr6_3:9896732-9896751 Msa0938180:intron 35.0%
AACTTGGCATGGAAGATAAA+AGG + chr6_3:9896837-9896856 None:intergenic 35.0%
AAATTCGTGTAAACTTGGCA+TGG + chr6_3:9896848-9896867 None:intergenic 35.0%
AGTATATGCAAGCTATACAC+AGG + chr6_3:9896912-9896931 None:intergenic 35.0%
ATCAACTCTGTTCATGAACT+CGG + chr6_3:9896153-9896172 None:intergenic 35.0%
AATTAGCATTGTGAGGTTAC+AGG - chr6_3:9896211-9896230 Msa0938180:three_prime_UTR 35.0%
TTAGAATGGATCGATTAGTG+AGG + chr6_3:9896246-9896265 None:intergenic 35.0%
! TTACTCGGATTTTCATCTTG+CGG + chr6_3:9896519-9896538 None:intergenic 35.0%
ATCCAACTTCTTGGAAGTTT+AGG - chr6_3:9896550-9896569 Msa0938180:CDS 35.0%
TCCAACTTCTTGGAAGTTTA+GGG - chr6_3:9896551-9896570 Msa0938180:CDS 35.0%
TGTGGAAAATGAACTTGCAA+AGG - chr6_3:9896624-9896643 Msa0938180:intron 35.0%
GTGGAAAATGAACTTGCAAA+GGG - chr6_3:9896625-9896644 Msa0938180:intron 35.0%
!!! CTAAAAACTAGCTTTTGCTG+AGG + chr6_3:9896730-9896749 None:intergenic 35.0%
!!! CTCAGCAAAAGCTAGTTTTT+AGG - chr6_3:9896728-9896747 Msa0938180:intron 35.0%
!!! AGCAAAAGCTAGTTTTTAGG+AGG - chr6_3:9896731-9896750 Msa0938180:intron 35.0%
!!! GCAAAAGCTAGTTTTTAGGA+GGG - chr6_3:9896732-9896751 Msa0938180:intron 35.0%
AACTTGGCATGGAAGATAAA+AGG + chr6_3:9896837-9896856 None:intergenic 35.0%
AAATTCGTGTAAACTTGGCA+TGG + chr6_3:9896848-9896867 None:intergenic 35.0%
AGTATATGCAAGCTATACAC+AGG + chr6_3:9896912-9896931 None:intergenic 35.0%
GTGCACTATTCATGAACTCA+GGG + chr6_3:9896064-9896083 None:intergenic 40.0%
GAATAGTGCACCGAAAGATT+TGG - chr6_3:9896073-9896092 Msa0938180:three_prime_UTR 40.0%
TCAACTCTGTTCATGAACTC+GGG + chr6_3:9896152-9896171 None:intergenic 40.0%
!! ATTATTTGGTGACGGTCAAG+AGG + chr6_3:9896194-9896213 None:intergenic 40.0%
ACCCTAAACTTCCAAGAAGT+TGG + chr6_3:9896555-9896574 None:intergenic 40.0%
GCGAGTGATTCAACAAAATG+TGG - chr6_3:9896606-9896625 Msa0938180:intron 40.0%
TCGAGGAGAATTTAAGGAAG+AGG - chr6_3:9896654-9896673 Msa0938180:intron 40.0%
AATCTACTGCTAAAGACAGC+AGG - chr6_3:9896770-9896789 Msa0938180:intron 40.0%
ATCTACTGCTAAAGACAGCA+GGG - chr6_3:9896771-9896790 Msa0938180:intron 40.0%
GTGCACTATTCATGAACTCA+GGG + chr6_3:9896064-9896083 None:intergenic 40.0%
GAATAGTGCACCGAAAGATT+TGG - chr6_3:9896073-9896092 Msa0938180:three_prime_UTR 40.0%
TCAACTCTGTTCATGAACTC+GGG + chr6_3:9896152-9896171 None:intergenic 40.0%
!! ATTATTTGGTGACGGTCAAG+AGG + chr6_3:9896194-9896213 None:intergenic 40.0%
ACCCTAAACTTCCAAGAAGT+TGG + chr6_3:9896555-9896574 None:intergenic 40.0%
GCGAGTGATTCAACAAAATG+TGG - chr6_3:9896606-9896625 Msa0938180:intron 40.0%
TCGAGGAGAATTTAAGGAAG+AGG - chr6_3:9896654-9896673 Msa0938180:intron 40.0%
AATCTACTGCTAAAGACAGC+AGG - chr6_3:9896770-9896789 Msa0938180:intron 40.0%
ATCTACTGCTAAAGACAGCA+GGG - chr6_3:9896771-9896790 Msa0938180:intron 40.0%
GATGCTGCAAGATCCATCTT+TGG - chr6_3:9896038-9896057 Msa0938180:three_prime_UTR 45.0%
GGTGCACTATTCATGAACTC+AGG + chr6_3:9896065-9896084 None:intergenic 45.0%
AACCATGAAGCAGCGTGTAA+TGG - chr6_3:9896103-9896122 Msa0938180:three_prime_UTR 45.0%
ATCCATTACACGCTGCTTCA+TGG + chr6_3:9896108-9896127 None:intergenic 45.0%
CAACTCTGTTCATGAACTCG+GGG + chr6_3:9896151-9896170 None:intergenic 45.0%
GGATAGCAGGTTCTGTTACT+CGG + chr6_3:9896534-9896553 None:intergenic 45.0%
GAACCTGCTATCCAACTTCT+TGG - chr6_3:9896541-9896560 Msa0938180:CDS 45.0%
CTTCCAAGAAGTTGGATAGC+AGG + chr6_3:9896547-9896566 None:intergenic 45.0%
TTAGGGTACGATCCAAGCTA+TGG - chr6_3:9896568-9896587 Msa0938180:CDS 45.0%
TGTTGAATCACTCGCTTCAC+CGG + chr6_3:9896601-9896620 None:intergenic 45.0%
!! CTTGCAAAGGGTATTCTTCG+AGG - chr6_3:9896637-9896656 Msa0938180:intron 45.0%
GAAGTCACAATACTCGCCAA+TGG - chr6_3:9896697-9896716 Msa0938180:intron 45.0%
GATGCTGCAAGATCCATCTT+TGG - chr6_3:9896038-9896057 Msa0938180:three_prime_UTR 45.0%
GGTGCACTATTCATGAACTC+AGG + chr6_3:9896065-9896084 None:intergenic 45.0%
AACCATGAAGCAGCGTGTAA+TGG - chr6_3:9896103-9896122 Msa0938180:three_prime_UTR 45.0%
ATCCATTACACGCTGCTTCA+TGG + chr6_3:9896108-9896127 None:intergenic 45.0%
CAACTCTGTTCATGAACTCG+GGG + chr6_3:9896151-9896170 None:intergenic 45.0%
GGATAGCAGGTTCTGTTACT+CGG + chr6_3:9896534-9896553 None:intergenic 45.0%
GAACCTGCTATCCAACTTCT+TGG - chr6_3:9896541-9896560 Msa0938180:CDS 45.0%
CTTCCAAGAAGTTGGATAGC+AGG + chr6_3:9896547-9896566 None:intergenic 45.0%
TTAGGGTACGATCCAAGCTA+TGG - chr6_3:9896568-9896587 Msa0938180:CDS 45.0%
TGTTGAATCACTCGCTTCAC+CGG + chr6_3:9896601-9896620 None:intergenic 45.0%
!! CTTGCAAAGGGTATTCTTCG+AGG - chr6_3:9896637-9896656 Msa0938180:intron 45.0%
GAAGTCACAATACTCGCCAA+TGG - chr6_3:9896697-9896716 Msa0938180:intron 45.0%
CATGAACTCAGGGCCAAAGA+TGG + chr6_3:9896054-9896073 None:intergenic 50.0%
! TCCAAGCTATGGTGCTAGAC+CGG - chr6_3:9896579-9896598 Msa0938180:CDS 50.0%
TTGCTGAGGACGTTGACCAT+TGG + chr6_3:9896716-9896735 None:intergenic 50.0%
CATGAACTCAGGGCCAAAGA+TGG + chr6_3:9896054-9896073 None:intergenic 50.0%
! TCCAAGCTATGGTGCTAGAC+CGG - chr6_3:9896579-9896598 Msa0938180:CDS 50.0%
TTGCTGAGGACGTTGACCAT+TGG + chr6_3:9896716-9896735 None:intergenic 50.0%
ACCGGTCTAGCACCATAGCT+TGG + chr6_3:9896583-9896602 None:intergenic 55.0%
ACCGGTCTAGCACCATAGCT+TGG + chr6_3:9896583-9896602 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 9896020 9897084 9896020 ID=Msa0938180;Name=Msa0938180
chr6_3 mRNA 9896020 9897084 9896020 ID=Msa0938180-mRNA-1;Parent=Msa0938180;Name=Msa0938180-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|1|0.5|1|1|0.5|2|268|180
chr6_3 exon 9896020 9896617 9896020 ID=Msa0938180-mRNA-1:exon:3170;Parent=Msa0938180-mRNA-1
chr6_3 exon 9896872 9897084 9896872 ID=Msa0938180-mRNA-1:exon:3169;Parent=Msa0938180-mRNA-1
chr6_3 CDS 9896872 9897084 9896872 ID=Msa0938180-mRNA-1:cds;Parent=Msa0938180-mRNA-1
chr6_3 CDS 9896288 9896617 9896288 ID=Msa0938180-mRNA-1:cds;Parent=Msa0938180-mRNA-1
chr6_3 three_prime_UTR 9896020 9896287 9896020 ID=Msa0938180-mRNA-1:three_prime_utr;Parent=Msa0938180-mRNA-1
Gene Sequence

>Msa0938180

ATGAAGCAGCGTGTAATAGATGCTGCAAGATCCATCTTTGGCCCTGAGTTCATGAATAGTGCACCGAAAGATTTGGCATATGAAACCATGAAGCAGCGTGTAATGGATGCTGCAAGATTCATCTTTCGCCCCGAGTTCATGAACAGAGTTGATGAGTATATTGTTTTTCGGCCTCTTGACCGTCACCAAATAATTAGCATTGTGAGGTTACAGTTGGAGCGCGTGCAGAAGAGAATTGCTGACCGCAAGATGAAAATCCGAGTAACAGAACCTGCTATCCAACTTCTTGGAAGTTTAGGGTACGATCCAAGCTATGGTGCTAGACCGGTGAAGCGAGTGATTCAACAAAATGTGGAAAATGAACTTGCAAAGGGTATTCTTCGAGGAGAATTTAAGGAAGAGGACACAATTTTAGTCGACACAGAAGTCACAATACTCGCCAATGGTCAACGTCCTCAGCAAAAGCTAGTTTTTAGGAGGGTTGAAGCTGATTCAGAATCTACTGCTAAAGACAGCAGGGAAAGTTTTCCACAGATTCTATGA
Protein sequence

>Msa0938180

MKQRVIDAARSIFGPEFMNSAPKDLAYETMKQRVMDAARFIFRPEFMNRVDEYIVFRPLDRHQIISIVRLQLERVQKRIADRKMKIRVTEPAIQLLGSLGYDPSYGARPVKRVIQQNVENELAKGILRGEFKEEDTILVDTEVTILANGQRPQQKLVFRRVEADSESTAKDSRESFPQIL*