AlfalfaGEDB | Msa0906160

M. sativa cultivar zhongmu-4 / Msa0906160

KEGG: K03695
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0006996 GO:0008150 GO:0009266 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009657 GO:0009658 GO:0009987 GO:0016043 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0071840
PFAM: PF10431.12

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0906160	XP_003618609.1	97.248	109	3	0	3	111	866	974	1.50e-63	218

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0906160	sp\|Q9LF37\|CLPB3_ARATH	75.532	94	23	0	3	96	863	956	2.57e-44	156

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0063850	Msa0906160	0.825269	5.138411e-54	-8.615850e-47
Msa0065190	Msa0906160	0.819960	8.824985e-53	-8.615850e-47
Msa0110500	Msa0906160	0.820256	7.549011e-53	-8.615850e-47
Msa0118250	Msa0906160	0.809690	1.670314e-50	-8.615850e-47
Msa0626900	Msa0906160	0.808896	2.472244e-50	-8.615850e-47
Msa0675250	Msa0906160	0.829208	5.854377e-55	-8.615850e-47
Msa0747900	Msa0906160	0.806089	9.742286e-50	-8.615850e-47
Msa0749160	Msa0906160	0.821239	4.486703e-53	-8.615850e-47
Msa0752830	Msa0906160	0.826343	2.858291e-54	-8.615850e-47
Msa0175100	Msa0906160	0.824612	7.344180e-54	-8.615850e-47
Msa0463140	Msa0906160	0.803926	2.761429e-49	-8.615850e-47
Msa0349450	Msa0906160	0.815223	1.032115e-51	-8.615850e-47
Msa0565930	Msa0906160	0.815172	1.059043e-51	-8.615850e-47
Msa0901660	Msa0906160	0.824196	9.200684e-54	-8.615850e-47
Msa0906160	Msa0929820	0.814880	1.230010e-51	-8.615850e-47
Msa0906160	Msa0938180	0.890915	6.396845e-74	-8.615850e-47
Msa0906160	Msa0975270	0.803564	3.281804e-49	-8.615850e-47
Msa0906160	Msa1067910	0.821872	3.205076e-53	-8.615850e-47
Msa0906160	Msa1085370	0.801262	9.785266e-49	-8.615850e-47
Msa0906160	Msa1143820	0.813076	3.073503e-51	-8.615850e-47
Msa0906160	Msa1185030	0.853629	2.105555e-61	-8.615850e-47
Msa0906160	Msa1186010	0.813442	2.554884e-51	-8.615850e-47
Msa0906160	Msa1239000	0.830863	2.310554e-55	-8.615850e-47
Msa0906160	Msa1240240	0.809235	2.091330e-50	-8.615850e-47
Msa0906160	Msa1285870	0.824660	7.158055e-54	-8.615850e-47
Msa0906160	Msa1299900	0.803646	3.155957e-49	-8.615850e-47
Msa0906160	Msa1364420	0.845905	3.034198e-59	-8.615850e-47
Msa0906160	Msa1395580	0.813879	2.046918e-51	-8.615850e-47
Msa0906160	Msa1465950	0.803952	2.727133e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0906160	MtrunA17_Chr6g0454071	95.556	90	4	3	92	866	955	4.62e-54	180
Msa0906160	MtrunA17_Chr7g0218421	80.374	107	21	3	109	870	976	6.71e-52	174
Msa0906160	MtrunA17_Chr3g0137071	52.174	92	44	3	94	879	970	7.80e-27	103

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0906160	AT5G15450.1	75.532	94	23	0	3	96	863	956	2.62e-45	156
Msa0906160	AT2G25140.1	50.000	96	42	2	3	98	870	959	7.06e-24	95.1
Msa0906160	AT5G51070.1	37.143	70	44	0	1	70	848	917	1.93e-11	59.7

Find 28 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATTTCAAATACTATTTCT+AGG	0.203814	6_2:+4726422	None:intergenic
ATCCAACTTCTTGGAAGTTT+AGG	0.235052	6_2:-4726894	Msa0906160:CDS
TATTCTTCGAGGAGAATTTA+AGG	0.278524	6_2:-4726796	Msa0906160:CDS
TCCAACTTCTTGGAAGTTTA+GGG	0.290774	6_2:-4726893	Msa0906160:CDS
GAACCTGCTATCCAACTTCT+TGG	0.317322	6_2:-4726903	Msa0906160:CDS
AACTTGGCATGGAAGATAAA+AGG	0.330056	6_2:+4726610	None:intergenic
ATCGTTCTGTTATTATAGAT+TGG	0.336034	6_2:-4726567	Msa0906160:three_prime_UTR
ACCCTAAACTTCCAAGAAGT+TGG	0.420686	6_2:+4726892	None:intergenic
GTTATTATAGATTGGAACTC+TGG	0.429182	6_2:-4726559	Msa0906160:three_prime_UTR
TCTCAAAATTCGTGTAAACT+TGG	0.442353	6_2:+4726594	None:intergenic
CTTCCAAGAAGTTGGATAGC+AGG	0.453391	6_2:+4726900	None:intergenic
TCGAGGAGAATTTAAGGAAG+AGG	0.483569	6_2:-4726790	Msa0906160:CDS
TGTTGAATCACTCGCTTCAC+CGG	0.489454	6_2:+4726846	None:intergenic
GGATAGCAGGTTCTGTTACT+CGG	0.501544	6_2:+4726913	None:intergenic
ACCGGTCTAGCACCATAGCT+TGG	0.505665	6_2:+4726864	None:intergenic
TTGCTGAGGACGTTGACCAT+TGG	0.509376	6_2:+4726731	None:intergenic
AATCTACTGCTAAAGACAGC+AGG	0.516797	6_2:-4726674	Msa0906160:CDS
AAATTTCTTCATAGAATCTG+TGG	0.517735	6_2:+4726642	None:intergenic
CTTGCAAAGGGTATTCTTCG+AGG	0.524988	6_2:-4726807	Msa0906160:CDS
TCCAAGCTATGGTGCTAGAC+CGG	0.530798	6_2:-4726865	Msa0906160:CDS
GTGGAAAATGAACTTGCAAA+GGG	0.543223	6_2:-4726819	Msa0906160:CDS
TGTGGAAAATGAACTTGCAA+AGG	0.545576	6_2:-4726820	Msa0906160:CDS
GCGAGTGATTCAACAAAATG+TGG	0.563340	6_2:-4726838	Msa0906160:CDS
AAATTCGTGTAAACTTGGCA+TGG	0.589380	6_2:+4726599	None:intergenic
TTAGGGTACGATCCAAGCTA+TGG	0.593819	6_2:-4726876	Msa0906160:CDS
ATCTACTGCTAAAGACAGCA+GGG	0.595696	6_2:-4726673	Msa0906160:CDS
GAAGTCACAATACTCGCCAA+TGG	0.657171	6_2:-4726747	Msa0906160:CDS
AGTATATGCAAGCTATACAC+AGG	0.666070	6_2:+4726535	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GTTTATATTTTACTTTATAT+TGG	-	chr6_2:4726851-4726870	Msa0906160:CDS	10.0%
!!!	TCATTTCAAATACTATTTCT+AGG	+	chr6_2:4726936-4726955	None:intergenic	20.0%
!	AAATTTCTTCATAGAATCTG+TGG	+	chr6_2:4726716-4726735	None:intergenic	25.0%
!	ATCGTTCTGTTATTATAGAT+TGG	-	chr6_2:4726788-4726807	Msa0906160:CDS	25.0%
	TATTCTTCGAGGAGAATTTA+AGG	-	chr6_2:4726559-4726578	Msa0906160:three_prime_UTR	30.0%
	TCTCAAAATTCGTGTAAACT+TGG	+	chr6_2:4726764-4726783	None:intergenic	30.0%
	GTTATTATAGATTGGAACTC+TGG	-	chr6_2:4726796-4726815	Msa0906160:CDS	30.0%
!	TTACTCGGATTTTCATCTTG+CGG	+	chr6_2:4726430-4726449	None:intergenic	35.0%
	ATCCAACTTCTTGGAAGTTT+AGG	-	chr6_2:4726461-4726480	Msa0906160:three_prime_UTR	35.0%
	TCCAACTTCTTGGAAGTTTA+GGG	-	chr6_2:4726462-4726481	Msa0906160:three_prime_UTR	35.0%
	TGTGGAAAATGAACTTGCAA+AGG	-	chr6_2:4726535-4726554	Msa0906160:three_prime_UTR	35.0%
	GTGGAAAATGAACTTGCAAA+GGG	-	chr6_2:4726536-4726555	Msa0906160:three_prime_UTR	35.0%
!!!	CTAAAAACTAGCTTTTGCTG+AGG	+	chr6_2:4726641-4726660	None:intergenic	35.0%
!!!	CTCAGCAAAAGCTAGTTTTT+AGG	-	chr6_2:4726639-4726658	Msa0906160:exon	35.0%
!!!	AGCAAAAGCTAGTTTTTAGG+AGG	-	chr6_2:4726642-4726661	Msa0906160:exon	35.0%
!!!	GCAAAAGCTAGTTTTTAGGA+GGG	-	chr6_2:4726643-4726662	Msa0906160:exon	35.0%
	AACTTGGCATGGAAGATAAA+AGG	+	chr6_2:4726748-4726767	None:intergenic	35.0%
	AAATTCGTGTAAACTTGGCA+TGG	+	chr6_2:4726759-4726778	None:intergenic	35.0%
	AGTATATGCAAGCTATACAC+AGG	+	chr6_2:4726823-4726842	None:intergenic	35.0%
	ACCCTAAACTTCCAAGAAGT+TGG	+	chr6_2:4726466-4726485	None:intergenic	40.0%
	GCGAGTGATTCAACAAAATG+TGG	-	chr6_2:4726517-4726536	Msa0906160:three_prime_UTR	40.0%
	TCGAGGAGAATTTAAGGAAG+AGG	-	chr6_2:4726565-4726584	Msa0906160:three_prime_UTR	40.0%
	AATCTACTGCTAAAGACAGC+AGG	-	chr6_2:4726681-4726700	Msa0906160:CDS	40.0%
	ATCTACTGCTAAAGACAGCA+GGG	-	chr6_2:4726682-4726701	Msa0906160:CDS	40.0%
	GGATAGCAGGTTCTGTTACT+CGG	+	chr6_2:4726445-4726464	None:intergenic	45.0%
	GAACCTGCTATCCAACTTCT+TGG	-	chr6_2:4726452-4726471	Msa0906160:three_prime_UTR	45.0%
	CTTCCAAGAAGTTGGATAGC+AGG	+	chr6_2:4726458-4726477	None:intergenic	45.0%
	TTAGGGTACGATCCAAGCTA+TGG	-	chr6_2:4726479-4726498	Msa0906160:three_prime_UTR	45.0%
	TGTTGAATCACTCGCTTCAC+CGG	+	chr6_2:4726512-4726531	None:intergenic	45.0%
!!	CTTGCAAAGGGTATTCTTCG+AGG	-	chr6_2:4726548-4726567	Msa0906160:three_prime_UTR	45.0%
	GAAGTCACAATACTCGCCAA+TGG	-	chr6_2:4726608-4726627	Msa0906160:three_prime_UTR	45.0%
!	TCCAAGCTATGGTGCTAGAC+CGG	-	chr6_2:4726490-4726509	Msa0906160:three_prime_UTR	50.0%
	TTGCTGAGGACGTTGACCAT+TGG	+	chr6_2:4726627-4726646	None:intergenic	50.0%
	ACCGGTCTAGCACCATAGCT+TGG	+	chr6_2:4726494-4726513	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	4726382	4726995	4726382	ID=Msa0906160;Name=Msa0906160
chr6_2	mRNA	4726382	4726995	4726382	ID=Msa0906160-mRNA-1;Parent=Msa0906160;Name=Msa0906160-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0\|0\|0.5\|1\|1\|1\|2\|268\|111
chr6_2	exon	4726382	4726979	4726382	ID=Msa0906160-mRNA-1:exon:1390;Parent=Msa0906160-mRNA-1
chr6_2	exon	4726990	4726995	4726990	ID=Msa0906160-mRNA-1:exon:1389;Parent=Msa0906160-mRNA-1
chr6_2	CDS	4726990	4726995	4726990	ID=Msa0906160-mRNA-1:cds;Parent=Msa0906160-mRNA-1
chr6_2	CDS	4726650	4726979	4726650	ID=Msa0906160-mRNA-1:cds;Parent=Msa0906160-mRNA-1
chr6_2	three_prime_UTR	4726382	4726649	4726382	ID=Msa0906160-mRNA-1:three_prime_utr;Parent=Msa0906160-mRNA-1

Gene Sequence	>Msa0906160 ATGTTTTTGGAGCGCGTGCAGAAGAGAATTGCTGACCGCAAGATGAAAATCCGAGTAACAGAACCTGCTATCCAACTTCTTGGAAGTTTAGGGTACGATCCAAGCTATGGTGCTAGACCGGTGAAGCGAGTGATTCAACAAAATGTGGAAAATGAACTTGCAAAGGGTATTCTTCGAGGAGAATTTAAGGAAGAGGACACAATTTTAGTCGACACAGAAGTCACAATACTCGCCAATGGTCAACGTCCTCAGCAAAAGCTAGTTTTTAGGAGGGTTGAAGCTGATTCAGAATCTACTGCTAAAGACAGCAGGGAAAGTTTTCCACAGATTCTATGA
Protein sequence	>Msa0906160 MFLERVQKRIADRKMKIRVTEPAIQLLGSLGYDPSYGARPVKRVIQQNVENELAKGILRGEFKEEDTILVDTEVTILANGQRPQQKLVFRRVEADSESTAKDSRESFPQIL*