Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0941370 | A0A072TUT6 | 96.809 | 94 | 3 | 0 | 1 | 94 | 378 | 471 | 4.09e-56 | 189 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0941370 | Msa1033560 | 0.800082 | 1.702769e-48 | -8.615850e-47 |
| Msa0941370 | Msa1073350 | 0.802792 | 4.741504e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0941370 | MtrunA17_Chr6g0457291 | 95.775 | 71 | 3 | 0 | 24 | 94 | 1 | 71 | 1.27e-46 | 144 |
| Msa0941370 | MtrunA17_Chr7g0217051 | 56.989 | 93 | 40 | 0 | 1 | 93 | 374 | 466 | 3.40e-32 | 116 |
| Msa0941370 | MtrunA17_Chr4g0040261 | 58.065 | 93 | 39 | 0 | 1 | 93 | 404 | 496 | 4.95e-30 | 110 |
| Msa0941370 | MtrunA17_Chr4g0040251 | 58.889 | 90 | 37 | 0 | 1 | 90 | 371 | 460 | 1.13e-29 | 109 |
| Msa0941370 | MtrunA17_Chr4g0040221 | 54.839 | 93 | 42 | 0 | 3 | 95 | 373 | 465 | 2.35e-29 | 108 |
| Msa0941370 | MtrunA17_Chr4g0040001 | 55.556 | 90 | 40 | 0 | 1 | 90 | 376 | 465 | 1.72e-26 | 100 |
| Msa0941370 | MtrunA17_Chr4g0040231 | 52.688 | 93 | 44 | 0 | 3 | 95 | 378 | 470 | 1.42e-25 | 98.6 |
| Msa0941370 | MtrunA17_Chr7g0248011 | 50.000 | 86 | 43 | 0 | 1 | 86 | 372 | 457 | 4.30e-25 | 97.1 |
| Msa0941370 | MtrunA17_Chr6g0457271 | 87.234 | 47 | 6 | 0 | 1 | 47 | 313 | 359 | 8.13e-24 | 92.8 |
| Msa0941370 | MtrunA17_Chr4g0040011 | 52.308 | 65 | 31 | 0 | 1 | 65 | 384 | 448 | 4.00e-17 | 74.7 |
| Msa0941370 | MtrunA17_Chr4g0040271 | 73.913 | 46 | 12 | 0 | 1 | 46 | 370 | 415 | 7.13e-17 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAGTGTTGTATACAAATT+TGG | 0.148234 | 6_3:-15184966 | None:intergenic |
| ACAACAATTTCCAACATAAT+TGG | 0.229434 | 6_3:+15185001 | Msa0941370:CDS |
| TTGGCTAATCATCCTGTTAA+AGG | 0.239724 | 6_3:+15185043 | Msa0941370:CDS |
| GGAAAATTAAGAATTGCATT+TGG | 0.368242 | 6_3:+15185733 | Msa0941370:CDS |
| ATAAAATATAGTCCTTTAAC+AGG | 0.381985 | 6_3:-15185055 | None:intergenic |
| CATGTAGCTCATGATGGTTA+TGG | 0.400565 | 6_3:-15185710 | None:intergenic |
| ATTAAGAATTGCATTTGGAT+TGG | 0.402978 | 6_3:+15185738 | Msa0941370:CDS |
| GCCAAAGCCATTTGCTCCAC+TGG | 0.416711 | 6_3:-15185025 | None:intergenic |
| ACCAGTGGAGCAAATGGCTT+TGG | 0.424194 | 6_3:+15185024 | Msa0941370:CDS |
| CTCCACTGGTCCAATTATGT+TGG | 0.445672 | 6_3:-15185011 | None:intergenic |
| AATTGGACCAGTGGAGCAAA+TGG | 0.483501 | 6_3:+15185018 | Msa0941370:CDS |
| CATAACCATCATGAGCTACA+TGG | 0.490689 | 6_3:+15185711 | Msa0941370:CDS |
| ATTTACATACTTACCTCTGG+TGG | 0.555522 | 6_3:-15185088 | None:intergenic |
| TTCCAACATAATTGGACCAG+TGG | 0.585156 | 6_3:+15185009 | Msa0941370:CDS |
| ATAACCATCATGAGCTACAT+GGG | 0.598244 | 6_3:+15185712 | Msa0941370:CDS |
| TATGGTTGTTGGTCCACCAG+AGG | 0.625962 | 6_3:+15185075 | Msa0941370:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTTTTTTTTAATAAAA+GGG | + | chr6_3:15185564-15185583 | Msa0941370:intron | 0.0% |
| !!! | TATTTTTTTTTTTAATAAAA+GGG | + | chr6_3:15185564-15185583 | Msa0941370:intron | 0.0% |
| !! | ATAATTAATAATCTATCTAA+TGG | - | chr6_3:15185200-15185219 | None:intergenic | 10.0% |
| !! | AGAAAAGAATATTATTAATA+TGG | - | chr6_3:15185328-15185347 | None:intergenic | 10.0% |
| !! | AACAAAAATTATATATTGTT+TGG | + | chr6_3:15185393-15185412 | Msa0941370:intron | 10.0% |
| !! | ATAATTAATAATCTATCTAA+TGG | - | chr6_3:15185200-15185219 | None:intergenic | 10.0% |
| !! | AGAAAAGAATATTATTAATA+TGG | - | chr6_3:15185328-15185347 | None:intergenic | 10.0% |
| !! | AACAAAAATTATATATTGTT+TGG | + | chr6_3:15185393-15185412 | Msa0941370:intron | 10.0% |
| !! | AATTGAATGTATATACTTTA+TGG | + | chr6_3:15185122-15185141 | Msa0941370:intron | 15.0% |
| !! | ATATAGTAGGTAAATTTATT+AGG | + | chr6_3:15185516-15185535 | Msa0941370:intron | 15.0% |
| !!! | TGTATGATATTATTTTTTGT+TGG | + | chr6_3:15185672-15185691 | Msa0941370:intron | 15.0% |
| !!! | ATGTTTTCTTTGATTTTATT+TGG | - | chr6_3:15185848-15185867 | None:intergenic | 15.0% |
| !! | AATTGAATGTATATACTTTA+TGG | + | chr6_3:15185122-15185141 | Msa0941370:intron | 15.0% |
| !! | ATATAGTAGGTAAATTTATT+AGG | + | chr6_3:15185516-15185535 | Msa0941370:intron | 15.0% |
| !!! | TGTATGATATTATTTTTTGT+TGG | + | chr6_3:15185672-15185691 | Msa0941370:intron | 15.0% |
| !!! | ATGTTTTCTTTGATTTTATT+TGG | - | chr6_3:15185848-15185867 | None:intergenic | 15.0% |
| !! | ATAAAATATAGTCCTTTAAC+AGG | - | chr6_3:15185058-15185077 | None:intergenic | 20.0% |
| !!! | TGTTAAAGGACTATATTTTA+TGG | + | chr6_3:15185057-15185076 | Msa0941370:CDS | 20.0% |
| !! | TGTATATACTTTATGGAAAT+AGG | + | chr6_3:15185129-15185148 | Msa0941370:intron | 20.0% |
| !! | CATATTCTCTTTAACATTAA+TGG | - | chr6_3:15185237-15185256 | None:intergenic | 20.0% |
| !! | CTACAGTATTATTTAGAAAA+AGG | - | chr6_3:15185490-15185509 | None:intergenic | 20.0% |
| !! | TATTCCAATAAATAATAGCA+TGG | + | chr6_3:15185628-15185647 | Msa0941370:intron | 20.0% |
| !! | AATTCCATGCTATTATTTAT+TGG | - | chr6_3:15185635-15185654 | None:intergenic | 20.0% |
| !! | ATAAAATATAGTCCTTTAAC+AGG | - | chr6_3:15185058-15185077 | None:intergenic | 20.0% |
| !!! | TGTTAAAGGACTATATTTTA+TGG | + | chr6_3:15185057-15185076 | Msa0941370:CDS | 20.0% |
| !! | TGTATATACTTTATGGAAAT+AGG | + | chr6_3:15185129-15185148 | Msa0941370:intron | 20.0% |
| !! | CATATTCTCTTTAACATTAA+TGG | - | chr6_3:15185237-15185256 | None:intergenic | 20.0% |
| !! | CTACAGTATTATTTAGAAAA+AGG | - | chr6_3:15185490-15185509 | None:intergenic | 20.0% |
| !! | TATTCCAATAAATAATAGCA+TGG | + | chr6_3:15185628-15185647 | Msa0941370:intron | 20.0% |
| !! | AATTCCATGCTATTATTTAT+TGG | - | chr6_3:15185635-15185654 | None:intergenic | 20.0% |
| !!! | CAAAGTGTTGTATACAAATT+TGG | - | chr6_3:15184969-15184988 | None:intergenic | 25.0% |
| ! | ACAACAATTTCCAACATAAT+TGG | + | chr6_3:15185001-15185020 | Msa0941370:CDS | 25.0% |
| !! | TACTTTGAGTTAAATTTGGT+GGG | - | chr6_3:15185461-15185480 | None:intergenic | 25.0% |
| !! | ATACTTTGAGTTAAATTTGG+TGG | - | chr6_3:15185462-15185481 | None:intergenic | 25.0% |
| !! | GTCATACTTTGAGTTAAATT+TGG | - | chr6_3:15185465-15185484 | None:intergenic | 25.0% |
| ! | CAGTATTATTTAGAAAAAGG+TGG | - | chr6_3:15185487-15185506 | None:intergenic | 25.0% |
| ! | ACTGTAGTTGTAAATATAGT+AGG | + | chr6_3:15185503-15185522 | Msa0941370:intron | 25.0% |
| ! | GTAGGTAAATTTATTAGGAA+AGG | + | chr6_3:15185521-15185540 | Msa0941370:intron | 25.0% |
| ! | GGAAAATTAAGAATTGCATT+TGG | + | chr6_3:15185733-15185752 | Msa0941370:CDS | 25.0% |
| ! | ATTAAGAATTGCATTTGGAT+TGG | + | chr6_3:15185738-15185757 | Msa0941370:CDS | 25.0% |
| !!! | CAAAGTGTTGTATACAAATT+TGG | - | chr6_3:15184969-15184988 | None:intergenic | 25.0% |
| ! | ACAACAATTTCCAACATAAT+TGG | + | chr6_3:15185001-15185020 | Msa0941370:CDS | 25.0% |
| !! | TACTTTGAGTTAAATTTGGT+GGG | - | chr6_3:15185461-15185480 | None:intergenic | 25.0% |
| !! | ATACTTTGAGTTAAATTTGG+TGG | - | chr6_3:15185462-15185481 | None:intergenic | 25.0% |
| !! | GTCATACTTTGAGTTAAATT+TGG | - | chr6_3:15185465-15185484 | None:intergenic | 25.0% |
| ! | CAGTATTATTTAGAAAAAGG+TGG | - | chr6_3:15185487-15185506 | None:intergenic | 25.0% |
| ! | ACTGTAGTTGTAAATATAGT+AGG | + | chr6_3:15185503-15185522 | Msa0941370:intron | 25.0% |
| ! | GTAGGTAAATTTATTAGGAA+AGG | + | chr6_3:15185521-15185540 | Msa0941370:intron | 25.0% |
| ! | GGAAAATTAAGAATTGCATT+TGG | + | chr6_3:15185733-15185752 | Msa0941370:CDS | 25.0% |
| ! | ATTAAGAATTGCATTTGGAT+TGG | + | chr6_3:15185738-15185757 | Msa0941370:CDS | 25.0% |
| ! | GGACTATATTTTATGGTTGT+TGG | + | chr6_3:15185064-15185083 | Msa0941370:CDS | 30.0% |
| ATCATTTACATACTTACCTC+TGG | - | chr6_3:15185094-15185113 | None:intergenic | 30.0% | |
| !!! | TTGTTTGGTTTTTTCTCCAA+TGG | + | chr6_3:15185408-15185427 | Msa0941370:intron | 30.0% |
| GCAAAAATTCATTTCATGCA+TGG | + | chr6_3:15185780-15185799 | Msa0941370:CDS | 30.0% | |
| ! | GGACTATATTTTATGGTTGT+TGG | + | chr6_3:15185064-15185083 | Msa0941370:CDS | 30.0% |
| ATCATTTACATACTTACCTC+TGG | - | chr6_3:15185094-15185113 | None:intergenic | 30.0% | |
| !!! | TTGTTTGGTTTTTTCTCCAA+TGG | + | chr6_3:15185408-15185427 | Msa0941370:intron | 30.0% |
| GCAAAAATTCATTTCATGCA+TGG | + | chr6_3:15185780-15185799 | Msa0941370:CDS | 30.0% | |
| TTGGCTAATCATCCTGTTAA+AGG | + | chr6_3:15185043-15185062 | Msa0941370:CDS | 35.0% | |
| ATTTACATACTTACCTCTGG+TGG | - | chr6_3:15185091-15185110 | None:intergenic | 35.0% | |
| !!! | TTTGGTTTTTTCTCCAATGG+TGG | + | chr6_3:15185411-15185430 | Msa0941370:intron | 35.0% |
| ATAACCATCATGAGCTACAT+GGG | + | chr6_3:15185712-15185731 | Msa0941370:CDS | 35.0% | |
| TTGGCTAATCATCCTGTTAA+AGG | + | chr6_3:15185043-15185062 | Msa0941370:CDS | 35.0% | |
| ATTTACATACTTACCTCTGG+TGG | - | chr6_3:15185091-15185110 | None:intergenic | 35.0% | |
| !!! | TTTGGTTTTTTCTCCAATGG+TGG | + | chr6_3:15185411-15185430 | Msa0941370:intron | 35.0% |
| ATAACCATCATGAGCTACAT+GGG | + | chr6_3:15185712-15185731 | Msa0941370:CDS | 35.0% | |
| TTCCAACATAATTGGACCAG+TGG | + | chr6_3:15185009-15185028 | Msa0941370:CDS | 40.0% | |
| ! | ATCCATCATTTTCACCATGC+TGG | + | chr6_3:15185157-15185176 | Msa0941370:intron | 40.0% |
| !! | TTAAATTTGGTGGGCTCAAG+TGG | - | chr6_3:15185452-15185471 | None:intergenic | 40.0% |
| CATGTAGCTCATGATGGTTA+TGG | - | chr6_3:15185713-15185732 | None:intergenic | 40.0% | |
| CATAACCATCATGAGCTACA+TGG | + | chr6_3:15185711-15185730 | Msa0941370:CDS | 40.0% | |
| ! | TTTTCCCATGTAGCTCATGA+TGG | - | chr6_3:15185719-15185738 | None:intergenic | 40.0% |
| TTCCAACATAATTGGACCAG+TGG | + | chr6_3:15185009-15185028 | Msa0941370:CDS | 40.0% | |
| ! | ATCCATCATTTTCACCATGC+TGG | + | chr6_3:15185157-15185176 | Msa0941370:intron | 40.0% |
| !! | TTAAATTTGGTGGGCTCAAG+TGG | - | chr6_3:15185452-15185471 | None:intergenic | 40.0% |
| CATGTAGCTCATGATGGTTA+TGG | - | chr6_3:15185713-15185732 | None:intergenic | 40.0% | |
| CATAACCATCATGAGCTACA+TGG | + | chr6_3:15185711-15185730 | Msa0941370:CDS | 40.0% | |
| ! | TTTTCCCATGTAGCTCATGA+TGG | - | chr6_3:15185719-15185738 | None:intergenic | 40.0% |
| CTCCACTGGTCCAATTATGT+TGG | - | chr6_3:15185014-15185033 | None:intergenic | 45.0% | |
| AATTGGACCAGTGGAGCAAA+TGG | + | chr6_3:15185018-15185037 | Msa0941370:CDS | 45.0% | |
| CACCAGCATGGTGAAAATGA+TGG | - | chr6_3:15185162-15185181 | None:intergenic | 45.0% | |
| !! | AGAAGCATCTTTCACCAGCA+TGG | - | chr6_3:15185174-15185193 | None:intergenic | 45.0% |
| !!! | GGTTTTTTCTCCAATGGTGG+TGG | + | chr6_3:15185414-15185433 | Msa0941370:intron | 45.0% |
| GGCTCAAGTGGTATCCAAAA+AGG | - | chr6_3:15185440-15185459 | None:intergenic | 45.0% | |
| TGGTTATGGTTAGACTCTGC+AGG | - | chr6_3:15185699-15185718 | None:intergenic | 45.0% | |
| CTCCACTGGTCCAATTATGT+TGG | - | chr6_3:15185014-15185033 | None:intergenic | 45.0% | |
| AATTGGACCAGTGGAGCAAA+TGG | + | chr6_3:15185018-15185037 | Msa0941370:CDS | 45.0% | |
| CACCAGCATGGTGAAAATGA+TGG | - | chr6_3:15185162-15185181 | None:intergenic | 45.0% | |
| !! | AGAAGCATCTTTCACCAGCA+TGG | - | chr6_3:15185174-15185193 | None:intergenic | 45.0% |
| !!! | GGTTTTTTCTCCAATGGTGG+TGG | + | chr6_3:15185414-15185433 | Msa0941370:intron | 45.0% |
| GGCTCAAGTGGTATCCAAAA+AGG | - | chr6_3:15185440-15185459 | None:intergenic | 45.0% | |
| TGGTTATGGTTAGACTCTGC+AGG | - | chr6_3:15185699-15185718 | None:intergenic | 45.0% | |
| !!! | GTATTTTTTTTTTTAATAAA+AGG | + | chr6_3:15185563-15185582 | Msa0941370:intron | 5.0% |
| !!! | GTATTTTTTTTTTTAATAAA+AGG | + | chr6_3:15185563-15185582 | Msa0941370:intron | 5.0% |
| ACCAGTGGAGCAAATGGCTT+TGG | + | chr6_3:15185024-15185043 | Msa0941370:CDS | 50.0% | |
| TATGGTTGTTGGTCCACCAG+AGG | + | chr6_3:15185075-15185094 | Msa0941370:CDS | 50.0% | |
| !!! | TCCAATGGTGGTGGCCTTTT+TGG | + | chr6_3:15185423-15185442 | Msa0941370:intron | 50.0% |
| TCCAAAAAGGCCACCACCAT+TGG | - | chr6_3:15185427-15185446 | None:intergenic | 50.0% | |
| ACCAGTGGAGCAAATGGCTT+TGG | + | chr6_3:15185024-15185043 | Msa0941370:CDS | 50.0% | |
| TATGGTTGTTGGTCCACCAG+AGG | + | chr6_3:15185075-15185094 | Msa0941370:CDS | 50.0% | |
| !!! | TCCAATGGTGGTGGCCTTTT+TGG | + | chr6_3:15185423-15185442 | Msa0941370:intron | 50.0% |
| TCCAAAAAGGCCACCACCAT+TGG | - | chr6_3:15185427-15185446 | None:intergenic | 50.0% | |
| GCCAAAGCCATTTGCTCCAC+TGG | - | chr6_3:15185028-15185047 | None:intergenic | 55.0% | |
| GCCAAAGCCATTTGCTCCAC+TGG | - | chr6_3:15185028-15185047 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 15184959 | 15185875 | 15184959 | ID=Msa0941370;Name=Msa0941370 |
| chr6_3 | mRNA | 15184959 | 15185875 | 15184959 | ID=Msa0941370-mRNA-1;Parent=Msa0941370;Name=Msa0941370-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0.5|1|1|1|2|23|95 |
| chr6_3 | exon | 15184959 | 15185096 | 15184959 | ID=Msa0941370-mRNA-1:exon:4592;Parent=Msa0941370-mRNA-1 |
| chr6_3 | exon | 15185703 | 15185875 | 15185703 | ID=Msa0941370-mRNA-1:exon:4593;Parent=Msa0941370-mRNA-1 |
| chr6_3 | CDS | 15184959 | 15185096 | 15184959 | ID=Msa0941370-mRNA-1:cds;Parent=Msa0941370-mRNA-1 |
| chr6_3 | CDS | 15185703 | 15185852 | 15185703 | ID=Msa0941370-mRNA-1:cds;Parent=Msa0941370-mRNA-1 |
| chr6_3 | three_prime_UTR | 15185853 | 15185875 | 15185853 | ID=Msa0941370-mRNA-1:three_prime_utr;Parent=Msa0941370-mRNA-1 |
| Gene Sequence |
| Protein sequence |
