Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0942760 | sp|O82081|UCC1_ARATH | 36.735 | 147 | 91 | 2 | 4 | 149 | 5 | 150 | 3.96e-31 | 116 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0942760 | G7KNP1 | 86.592 | 179 | 23 | 1 | 1 | 178 | 1 | 179 | 5.27e-85 | 257 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0942760 | Msa1369030 | 0.804236 | 2.379871e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0942760 | MtrunA17_Chr6g0459441 | 86.592 | 179 | 23 | 1 | 1 | 178 | 1 | 179 | 1.01e-88 | 257 |
| Msa0942760 | MtrunA17_Chr2g0323021 | 41.549 | 142 | 77 | 3 | 1 | 137 | 1 | 141 | 8.68e-32 | 114 |
| Msa0942760 | MtrunA17_Chr6g0481491 | 39.474 | 114 | 68 | 1 | 7 | 120 | 10 | 122 | 1.30e-30 | 110 |
| Msa0942760 | MtrunA17_Chr5g0394321 | 35.754 | 179 | 104 | 6 | 4 | 173 | 6 | 182 | 9.35e-28 | 102 |
| Msa0942760 | MtrunA17_Chr2g0289741 | 31.176 | 170 | 107 | 4 | 7 | 171 | 7 | 171 | 1.14e-26 | 99.4 |
| Msa0942760 | MtrunA17_Chr8g0335531 | 38.202 | 178 | 88 | 6 | 8 | 166 | 9 | 183 | 5.32e-26 | 98.2 |
| Msa0942760 | MtrunA17_Chr3g0132431 | 36.420 | 162 | 85 | 3 | 6 | 150 | 7 | 167 | 2.46e-25 | 96.3 |
| Msa0942760 | MtrunA17_Chr8g0335571 | 46.087 | 115 | 57 | 3 | 8 | 120 | 9 | 120 | 5.23e-25 | 95.9 |
| Msa0942760 | MtrunA17_Chr3g0132441 | 40.870 | 115 | 67 | 1 | 6 | 120 | 7 | 120 | 1.41e-23 | 92.0 |
| Msa0942760 | MtrunA17_Chr1g0196801 | 29.936 | 157 | 107 | 2 | 22 | 178 | 20 | 173 | 6.06e-22 | 87.4 |
| Msa0942760 | MtrunA17_Chr3g0132411 | 36.800 | 125 | 78 | 1 | 6 | 130 | 46 | 169 | 7.13e-22 | 87.0 |
| Msa0942760 | MtrunA17_Chr1g0177341 | 34.483 | 116 | 73 | 3 | 7 | 120 | 7 | 121 | 1.51e-20 | 83.2 |
| Msa0942760 | MtrunA17_Chr1g0177331 | 34.483 | 116 | 73 | 3 | 7 | 120 | 7 | 121 | 1.51e-20 | 83.2 |
| Msa0942760 | MtrunA17_Chr1g0205701 | 28.025 | 157 | 104 | 4 | 22 | 170 | 22 | 177 | 1.58e-20 | 84.0 |
| Msa0942760 | MtrunA17_Chr7g0253701 | 32.773 | 119 | 77 | 3 | 4 | 120 | 5 | 122 | 2.23e-20 | 82.8 |
| Msa0942760 | MtrunA17_Chr7g0253671 | 32.773 | 119 | 77 | 3 | 4 | 120 | 5 | 122 | 2.23e-20 | 82.8 |
| Msa0942760 | MtrunA17_Chr2g0331181 | 35.915 | 142 | 82 | 5 | 6 | 142 | 10 | 147 | 5.36e-20 | 82.8 |
| Msa0942760 | MtrunA17_Chr7g0253731 | 31.967 | 122 | 80 | 3 | 1 | 120 | 1 | 121 | 1.11e-19 | 81.3 |
| Msa0942760 | MtrunA17_Chr4g0039721 | 32.857 | 140 | 84 | 3 | 23 | 154 | 30 | 167 | 1.49e-19 | 81.3 |
| Msa0942760 | MtrunA17_Chr4g0064041 | 34.815 | 135 | 84 | 1 | 6 | 136 | 10 | 144 | 2.44e-19 | 84.0 |
| Msa0942760 | MtrunA17_Chr4g0064041 | 37.624 | 101 | 57 | 3 | 25 | 120 | 191 | 290 | 5.94e-17 | 77.0 |
| Msa0942760 | MtrunA17_Chr4g0001571 | 35.354 | 99 | 63 | 1 | 22 | 120 | 26 | 123 | 2.63e-19 | 80.5 |
| Msa0942760 | MtrunA17_Chr4g0033131 | 35.294 | 136 | 74 | 5 | 6 | 134 | 9 | 137 | 3.46e-19 | 80.1 |
| Msa0942760 | MtrunA17_Chr4g0033531 | 35.294 | 136 | 74 | 5 | 6 | 134 | 9 | 137 | 3.46e-19 | 80.1 |
| Msa0942760 | MtrunA17_Chr3g0132701 | 36.441 | 118 | 74 | 1 | 4 | 120 | 5 | 122 | 3.77e-19 | 81.3 |
| Msa0942760 | MtrunA17_Chr3g0132511 | 35.833 | 120 | 76 | 1 | 4 | 122 | 5 | 124 | 7.17e-19 | 80.5 |
| Msa0942760 | MtrunA17_Chr7g0253681 | 35.922 | 103 | 63 | 3 | 20 | 120 | 22 | 123 | 2.33e-18 | 77.8 |
| Msa0942760 | MtrunA17_Chr2g0306461 | 35.593 | 118 | 72 | 3 | 6 | 120 | 9 | 125 | 4.70e-18 | 77.8 |
| Msa0942760 | MtrunA17_Chr1g0211101 | 31.724 | 145 | 90 | 5 | 9 | 145 | 17 | 160 | 3.51e-17 | 75.1 |
| Msa0942760 | MtrunA17_Chr8g0380711 | 43.269 | 104 | 49 | 6 | 20 | 119 | 23 | 120 | 3.62e-17 | 73.6 |
| Msa0942760 | MtrunA17_Chr4g0062341 | 31.724 | 145 | 91 | 3 | 23 | 161 | 30 | 172 | 4.82e-17 | 74.7 |
| Msa0942760 | MtrunA17_Chr4g0062311 | 31.724 | 145 | 91 | 3 | 23 | 161 | 30 | 172 | 4.82e-17 | 74.7 |
| Msa0942760 | MtrunA17_Chr5g0440711 | 28.966 | 145 | 94 | 4 | 4 | 145 | 8 | 146 | 1.18e-16 | 72.8 |
| Msa0942760 | MtrunA17_Chr8g0360371 | 33.099 | 142 | 85 | 5 | 1 | 139 | 1 | 135 | 1.18e-16 | 75.9 |
| Msa0942760 | MtrunA17_Chr7g0253641 | 29.060 | 117 | 83 | 0 | 4 | 120 | 5 | 121 | 1.42e-16 | 74.3 |
| Msa0942760 | MtrunA17_Chr2g0301431 | 35.345 | 116 | 70 | 3 | 6 | 119 | 11 | 123 | 2.51e-16 | 71.2 |
| Msa0942760 | MtrunA17_Chr3g0125791 | 40.816 | 98 | 53 | 4 | 22 | 118 | 30 | 123 | 2.80e-16 | 71.2 |
| Msa0942760 | MtrunA17_Chr3g0125761 | 37.069 | 116 | 67 | 5 | 6 | 119 | 13 | 124 | 2.83e-16 | 71.2 |
| Msa0942760 | MtrunA17_Chr7g0253631 | 31.405 | 121 | 82 | 1 | 1 | 120 | 1 | 121 | 2.96e-16 | 73.9 |
| Msa0942760 | MtrunA17_Chr6g0453921 | 30.405 | 148 | 98 | 4 | 6 | 149 | 17 | 163 | 3.27e-16 | 72.4 |
| Msa0942760 | MtrunA17_Chr3g0136871 | 33.333 | 153 | 92 | 4 | 22 | 169 | 24 | 171 | 7.96e-16 | 71.6 |
| Msa0942760 | MtrunA17_Chr6g0453791 | 34.028 | 144 | 91 | 4 | 25 | 166 | 31 | 172 | 1.33e-15 | 70.9 |
| Msa0942760 | MtrunA17_Chr8g0385021 | 31.356 | 118 | 76 | 2 | 8 | 120 | 5 | 122 | 2.36e-15 | 72.0 |
| Msa0942760 | MtrunA17_Chr8g0385031 | 30.508 | 118 | 77 | 2 | 8 | 120 | 5 | 122 | 3.15e-15 | 71.6 |
| Msa0942760 | MtrunA17_Chr2g0324711 | 33.582 | 134 | 79 | 5 | 6 | 131 | 7 | 138 | 5.45e-15 | 71.6 |
| Msa0942760 | MtrunA17_Chr1g0150131 | 32.867 | 143 | 84 | 6 | 6 | 143 | 11 | 146 | 5.53e-15 | 69.3 |
| Msa0942760 | MtrunA17_Chr2g0324721 | 27.273 | 165 | 106 | 3 | 6 | 156 | 8 | 172 | 5.78e-15 | 69.7 |
| Msa0942760 | MtrunA17_Chr4g0040291 | 28.481 | 158 | 100 | 3 | 22 | 166 | 22 | 179 | 5.78e-15 | 69.3 |
| Msa0942760 | MtrunA17_Chr7g0253761 | 28.926 | 121 | 85 | 1 | 1 | 120 | 1 | 121 | 2.93e-14 | 67.4 |
| Msa0942760 | MtrunA17_Chr6g0458331 | 35.345 | 116 | 66 | 5 | 20 | 128 | 32 | 145 | 5.01e-14 | 66.2 |
| Msa0942760 | MtrunA17_Chr8g0387804 | 37.864 | 103 | 59 | 4 | 22 | 120 | 26 | 127 | 2.05e-13 | 63.9 |
| Msa0942760 | MtrunA17_Chr4g0041811 | 34.694 | 98 | 60 | 1 | 27 | 120 | 34 | 131 | 2.18e-13 | 65.9 |
| Msa0942760 | MtrunA17_Chr2g0319611 | 35.000 | 100 | 59 | 3 | 27 | 120 | 28 | 127 | 2.31e-13 | 66.2 |
| Msa0942760 | MtrunA17_Chr1g0188981 | 33.036 | 112 | 68 | 4 | 16 | 121 | 20 | 130 | 1.19e-12 | 63.2 |
| Msa0942760 | MtrunA17_Chr2g0317231 | 33.333 | 96 | 62 | 1 | 27 | 120 | 27 | 122 | 4.97e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0942760 | AT3G27200.1 | 62.609 | 115 | 43 | 0 | 6 | 120 | 7 | 121 | 1.21e-51 | 163 |
| Msa0942760 | AT2G32300.1 | 36.735 | 147 | 91 | 2 | 4 | 149 | 5 | 150 | 4.02e-32 | 116 |
| Msa0942760 | AT2G32300.2 | 37.241 | 145 | 89 | 2 | 6 | 149 | 37 | 180 | 9.88e-32 | 115 |
| Msa0942760 | AT5G07475.1 | 37.086 | 151 | 92 | 3 | 11 | 159 | 16 | 165 | 4.00e-30 | 109 |
| Msa0942760 | AT2G31050.1 | 41.600 | 125 | 70 | 3 | 9 | 131 | 13 | 136 | 7.54e-25 | 95.9 |
| Msa0942760 | AT2G26720.1 | 38.636 | 132 | 76 | 4 | 4 | 131 | 6 | 136 | 5.89e-24 | 93.6 |
| Msa0942760 | AT4G31840.1 | 36.000 | 175 | 99 | 8 | 7 | 175 | 10 | 177 | 9.35e-21 | 84.7 |
| Msa0942760 | AT5G53870.2 | 32.298 | 161 | 103 | 3 | 3 | 158 | 9 | 168 | 1.25e-20 | 87.8 |
| Msa0942760 | AT5G53870.1 | 32.298 | 161 | 103 | 3 | 3 | 158 | 9 | 168 | 1.68e-20 | 87.4 |
| Msa0942760 | AT1G72230.1 | 32.432 | 148 | 89 | 2 | 5 | 152 | 3 | 139 | 2.00e-19 | 81.3 |
| Msa0942760 | AT1G48940.1 | 33.333 | 186 | 105 | 6 | 1 | 177 | 1 | 176 | 3.84e-19 | 80.5 |
| Msa0942760 | AT2G27035.1 | 28.659 | 164 | 104 | 7 | 6 | 165 | 9 | 163 | 1.37e-18 | 78.6 |
| Msa0942760 | AT3G60270.1 | 40.594 | 101 | 59 | 1 | 20 | 120 | 21 | 120 | 2.20e-18 | 78.6 |
| Msa0942760 | AT1G45063.2 | 36.441 | 118 | 70 | 4 | 19 | 131 | 141 | 258 | 3.40e-18 | 79.7 |
| Msa0942760 | AT1G45063.2 | 31.304 | 115 | 77 | 2 | 8 | 120 | 12 | 126 | 2.07e-11 | 61.2 |
| Msa0942760 | AT1G22480.1 | 41.489 | 94 | 54 | 1 | 33 | 126 | 27 | 119 | 3.58e-18 | 77.8 |
| Msa0942760 | AT5G26330.1 | 33.613 | 119 | 73 | 4 | 7 | 120 | 4 | 121 | 3.69e-18 | 78.2 |
| Msa0942760 | AT3G18590.1 | 37.363 | 182 | 96 | 6 | 4 | 169 | 5 | 184 | 5.01e-18 | 77.8 |
| Msa0942760 | AT1G45063.1 | 36.441 | 118 | 70 | 4 | 19 | 131 | 141 | 258 | 5.61e-18 | 80.5 |
| Msa0942760 | AT1G45063.1 | 31.304 | 115 | 77 | 2 | 8 | 120 | 12 | 126 | 2.45e-11 | 61.6 |
| Msa0942760 | AT3G17675.1 | 35.593 | 118 | 71 | 4 | 6 | 120 | 12 | 127 | 1.00e-17 | 75.5 |
| Msa0942760 | AT3G60280.1 | 38.614 | 101 | 61 | 1 | 20 | 120 | 19 | 118 | 1.81e-17 | 77.0 |
| Msa0942760 | AT2G25060.1 | 40.496 | 121 | 58 | 6 | 6 | 120 | 19 | 131 | 6.93e-17 | 74.7 |
| Msa0942760 | AT5G20230.1 | 27.419 | 124 | 87 | 1 | 24 | 144 | 24 | 147 | 2.62e-16 | 73.6 |
| Msa0942760 | AT2G44790.1 | 40.000 | 95 | 54 | 2 | 33 | 125 | 36 | 129 | 2.83e-16 | 73.6 |
| Msa0942760 | AT4G12880.1 | 32.479 | 117 | 74 | 4 | 7 | 120 | 11 | 125 | 1.17e-15 | 70.5 |
| Msa0942760 | AT4G28365.1 | 38.129 | 139 | 76 | 6 | 27 | 158 | 31 | 166 | 6.04e-15 | 69.7 |
| Msa0942760 | AT4G27520.1 | 32.414 | 145 | 87 | 5 | 7 | 146 | 11 | 149 | 1.60e-14 | 70.5 |
| Msa0942760 | AT5G14345.1 | 34.074 | 135 | 75 | 6 | 7 | 133 | 1 | 129 | 2.42e-14 | 67.0 |
| Msa0942760 | AT4G32490.1 | 40.206 | 97 | 54 | 3 | 27 | 120 | 33 | 128 | 3.49e-14 | 68.2 |
| Msa0942760 | AT2G23990.1 | 39.216 | 102 | 54 | 5 | 26 | 120 | 28 | 128 | 4.22e-14 | 67.8 |
| Msa0942760 | AT5G57920.3 | 31.967 | 122 | 71 | 5 | 18 | 132 | 53 | 169 | 8.53e-14 | 67.0 |
| Msa0942760 | AT5G57920.1 | 32.927 | 82 | 55 | 0 | 39 | 120 | 42 | 123 | 8.87e-14 | 66.6 |
| Msa0942760 | AT3G53330.1 | 35.354 | 99 | 61 | 2 | 25 | 120 | 188 | 286 | 9.95e-14 | 68.2 |
| Msa0942760 | AT4G30590.1 | 36.000 | 100 | 57 | 4 | 27 | 120 | 30 | 128 | 1.14e-13 | 66.2 |
| Msa0942760 | AT5G57920.2 | 31.250 | 96 | 64 | 1 | 39 | 132 | 41 | 136 | 1.38e-13 | 65.9 |
| Msa0942760 | AT1G08500.1 | 30.723 | 166 | 91 | 5 | 3 | 145 | 8 | 172 | 2.06e-13 | 66.2 |
| Msa0942760 | AT1G17800.1 | 31.858 | 113 | 75 | 2 | 7 | 118 | 23 | 134 | 2.92e-13 | 63.9 |
| Msa0942760 | AT5G25090.1 | 31.667 | 120 | 72 | 3 | 6 | 120 | 12 | 126 | 8.72e-13 | 63.9 |
| Msa0942760 | AT2G02850.1 | 32.773 | 119 | 73 | 5 | 4 | 119 | 13 | 127 | 1.61e-12 | 62.0 |
| Msa0942760 | AT1G64640.1 | 31.967 | 122 | 77 | 4 | 19 | 135 | 27 | 147 | 2.62e-12 | 62.8 |
| Msa0942760 | AT3G20570.1 | 29.012 | 162 | 100 | 4 | 8 | 154 | 13 | 174 | 1.09e-11 | 61.2 |
Find 54 sgRNAs with CRISPR-Local
Find 152 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACATTCACTTACAATATA+TGG | 0.232970 | 6_3:-18103484 | None:intergenic |
| TTAATTGATTATATGAAGTT+TGG | 0.286852 | 6_3:+18103309 | Msa0942760:exon |
| TGGAAAAGATGTTGTGAAAT+TGG | 0.298026 | 6_3:+18103036 | Msa0942760:CDS |
| GACTTTGATTCATGGAGTTC+AGG | 0.309655 | 6_3:+18102357 | Msa0942760:CDS |
| GTACTTGTCTATAAAGAAAA+AGG | 0.309903 | 6_3:-18103560 | None:intergenic |
| CATCCTCAGACTTTGATTCA+TGG | 0.350091 | 6_3:+18102349 | Msa0942760:CDS |
| CAATACCTCCTTTGTGATCA+AGG | 0.383649 | 6_3:-18102286 | None:intergenic |
| CCAGTGCTTAAGGAGTTTAG+TGG | 0.413304 | 6_3:-18103016 | None:intergenic |
| GTAAAGATCACAGTAGGAAA+TGG | 0.422825 | 6_3:+18103121 | Msa0942760:CDS |
| CTTGATCACAAAGGAGGTAT+TGG | 0.426661 | 6_3:+18102287 | Msa0942760:CDS |
| CAGTGCTTAAGGAGTTTAGT+GGG | 0.442231 | 6_3:-18103015 | None:intergenic |
| CTGAAGATGTATCAGATGTA+GGG | 0.476612 | 6_3:-18103219 | None:intergenic |
| CGGGGAGGATGCAGAGGAAG+AGG | 0.479314 | 6_3:-18103146 | None:intergenic |
| GCTGAAGATGTATCAGATGT+AGG | 0.484922 | 6_3:-18103220 | None:intergenic |
| TACTTCACATGTGGTACCTT+AGG | 0.496106 | 6_3:+18103076 | Msa0942760:CDS |
| TCAGGTCAAACATTCAAAGT+TGG | 0.497486 | 6_3:+18102375 | Msa0942760:CDS |
| CAAAGAACAAATTGAACCAT+GGG | 0.498609 | 6_3:+18102228 | Msa0942760:exon |
| CATAATGTTGGTGGAAGTCA+AGG | 0.516548 | 6_3:+18102318 | Msa0942760:CDS |
| TTCAAAGTTGGAGATCAACT+TGG | 0.523708 | 6_3:+18102387 | Msa0942760:CDS |
| CCACTAAACTCCTTAAGCAC+TGG | 0.524379 | 6_3:+18103016 | Msa0942760:CDS |
| ACCTTAGGTCATTGTGGTCA+AGG | 0.528788 | 6_3:+18103091 | Msa0942760:CDS |
| ACCTTGACCACAATGACCTA+AGG | 0.541983 | 6_3:-18103092 | None:intergenic |
| TGTTGGTGGAAGTCAAGGTT+GGG | 0.547956 | 6_3:+18102323 | Msa0942760:CDS |
| ATGGTGATGATGAAGACGAC+GGG | 0.553747 | 6_3:-18103165 | None:intergenic |
| TCATTGTGGTCAAGGTATGA+AGG | 0.554331 | 6_3:+18103099 | Msa0942760:CDS |
| AATTATGTTCTTGCACCCCA+TGG | 0.558852 | 6_3:-18102244 | None:intergenic |
| CATGAATCAAAGTCTGAGGA+TGG | 0.566327 | 6_3:-18102348 | None:intergenic |
| CATGTGAAGTACCTTGTACC+TGG | 0.571913 | 6_3:-18103064 | None:intergenic |
| ATGTTGGTGGAAGTCAAGGT+TGG | 0.572733 | 6_3:+18102322 | Msa0942760:CDS |
| GCAACACAACATAATGTTGG+TGG | 0.579218 | 6_3:+18102309 | Msa0942760:CDS |
| ACTAGAGAGGTACAGTGGAA+TGG | 0.585410 | 6_3:+18103366 | Msa0942760:three_prime_UTR |
| ATGAAGGTAAAGATCACAGT+AGG | 0.591873 | 6_3:+18103115 | Msa0942760:CDS |
| CATATTTGCTGAATATGAAG+AGG | 0.592143 | 6_3:-18103402 | None:intergenic |
| ACAAAGAACAAATTGAACCA+TGG | 0.598455 | 6_3:+18102227 | Msa0942760:exon |
| AGACGACGGGGAGGATGCAG+AGG | 0.606454 | 6_3:-18103152 | None:intergenic |
| CAAAACAATCTCTAAAAGAG+TGG | 0.607452 | 6_3:+18102174 | None:intergenic |
| TTGGCAACACAACATAATGT+TGG | 0.615720 | 6_3:+18102306 | Msa0942760:CDS |
| AATTGGACAAGCCAGGTACA+AGG | 0.622027 | 6_3:+18103053 | Msa0942760:CDS |
| AGTGCTTAAGGAGTTTAGTG+GGG | 0.622685 | 6_3:-18103014 | None:intergenic |
| ACTCCATGAATCAAAGTCTG+AGG | 0.627608 | 6_3:-18102352 | None:intergenic |
| TGAAGATGTATCAGATGTAG+GGG | 0.632402 | 6_3:-18103218 | None:intergenic |
| TGTGGTACCTTAGGTCATTG+TGG | 0.638088 | 6_3:+18103085 | Msa0942760:CDS |
| GTTGTGAAATTGGACAAGCC+AGG | 0.645162 | 6_3:+18103046 | Msa0942760:CDS |
| TACATATACATACACTAGAG+AGG | 0.651560 | 6_3:+18103353 | Msa0942760:three_prime_UTR |
| AAGAGAAGCAAAGTATTGTG+AGG | 0.658665 | 6_3:-18103242 | None:intergenic |
| AGCTACCTTGATCACAAAGG+AGG | 0.662032 | 6_3:+18102281 | Msa0942760:CDS |
| GTTAGCTACCTTGATCACAA+AGG | 0.669540 | 6_3:+18102278 | Msa0942760:CDS |
| GTACACATCTATGCATAGTG+TGG | 0.679339 | 6_3:+18102946 | Msa0942760:CDS |
| CATACACTAGAGAGGTACAG+TGG | 0.685119 | 6_3:+18103361 | Msa0942760:three_prime_UTR |
| GGTACAAGGTACTTCACATG+TGG | 0.691210 | 6_3:+18103067 | Msa0942760:CDS |
| GATGGTGATGATGAAGACGA+CGG | 0.700742 | 6_3:-18103166 | None:intergenic |
| TGATGATGAAGACGACGGGG+AGG | 0.727873 | 6_3:-18103161 | None:intergenic |
| TGGTGATGATGAAGACGACG+GGG | 0.752589 | 6_3:-18103164 | None:intergenic |
| AAAGAACAAATTGAACCATG+GGG | 0.757129 | 6_3:+18102229 | Msa0942760:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTACATAATTATTTTTACT+TGG | + | chr6_3:18102497-18102516 | Msa0942760:intron | 10.0% |
| !!! | TTTACATAATTATTTTTACT+TGG | + | chr6_3:18102497-18102516 | Msa0942760:intron | 10.0% |
| !! | TTCTTATCAAAAAAATTTGT+TGG | + | chr6_3:18102415-18102434 | Msa0942760:intron | 15.0% |
| !!! | ATTTTTCTACTTTCTATTAT+AGG | - | chr6_3:18102451-18102470 | None:intergenic | 15.0% |
| !! | TTAATTGATTATATGAAGTT+TGG | + | chr6_3:18103309-18103328 | Msa0942760:exon | 15.0% |
| !! | TTCTTATCAAAAAAATTTGT+TGG | + | chr6_3:18102415-18102434 | Msa0942760:intron | 15.0% |
| !!! | ATTTTTCTACTTTCTATTAT+AGG | - | chr6_3:18102451-18102470 | None:intergenic | 15.0% |
| !! | TTAATTGATTATATGAAGTT+TGG | + | chr6_3:18103309-18103328 | Msa0942760:exon | 15.0% |
| !! | ATCAATCAAATATTGCTTAA+CGG | - | chr6_3:18102793-18102812 | None:intergenic | 20.0% |
| !! | ATCAATCAAATATTGCTTAA+CGG | - | chr6_3:18102793-18102812 | None:intergenic | 20.0% |
| !! | TTGTTTAAAACTTTGATTGG+TGG | - | chr6_3:18102576-18102595 | None:intergenic | 25.0% |
| !! | CCATTGTTTAAAACTTTGAT+TGG | - | chr6_3:18102579-18102598 | None:intergenic | 25.0% |
| !!! | CCAATCAAAGTTTTAAACAA+TGG | + | chr6_3:18102576-18102595 | Msa0942760:intron | 25.0% |
| ! | TTAAACAATGGTATTGTAGA+TGG | + | chr6_3:18102588-18102607 | Msa0942760:intron | 25.0% |
| ! | TAAACAATGGTATTGTAGAT+GGG | + | chr6_3:18102589-18102608 | Msa0942760:intron | 25.0% |
| ! | GTTAGTATATTCTCCTTAAT+CGG | - | chr6_3:18102728-18102747 | None:intergenic | 25.0% |
| ! | ACATCAAAGGTTATAAATTG+TGG | - | chr6_3:18102835-18102854 | None:intergenic | 25.0% |
| ! | TATAACCTTTGATGTGATTA+TGG | + | chr6_3:18102840-18102859 | Msa0942760:intron | 25.0% |
| ! | TATTTCCATAATCACATCAA+AGG | - | chr6_3:18102848-18102867 | None:intergenic | 25.0% |
| !!! | GCTAATATCACATTTTTTGT+AGG | - | chr6_3:18102993-18103012 | None:intergenic | 25.0% |
| ! | ACACATTCACTTACAATATA+TGG | - | chr6_3:18103487-18103506 | None:intergenic | 25.0% |
| !! | TTGTTTAAAACTTTGATTGG+TGG | - | chr6_3:18102576-18102595 | None:intergenic | 25.0% |
| !! | CCATTGTTTAAAACTTTGAT+TGG | - | chr6_3:18102579-18102598 | None:intergenic | 25.0% |
| !!! | CCAATCAAAGTTTTAAACAA+TGG | + | chr6_3:18102576-18102595 | Msa0942760:intron | 25.0% |
| ! | TTAAACAATGGTATTGTAGA+TGG | + | chr6_3:18102588-18102607 | Msa0942760:intron | 25.0% |
| ! | TAAACAATGGTATTGTAGAT+GGG | + | chr6_3:18102589-18102608 | Msa0942760:intron | 25.0% |
| ! | GTTAGTATATTCTCCTTAAT+CGG | - | chr6_3:18102728-18102747 | None:intergenic | 25.0% |
| ! | ACATCAAAGGTTATAAATTG+TGG | - | chr6_3:18102835-18102854 | None:intergenic | 25.0% |
| ! | TATAACCTTTGATGTGATTA+TGG | + | chr6_3:18102840-18102859 | Msa0942760:intron | 25.0% |
| ! | TATTTCCATAATCACATCAA+AGG | - | chr6_3:18102848-18102867 | None:intergenic | 25.0% |
| !!! | GCTAATATCACATTTTTTGT+AGG | - | chr6_3:18102993-18103012 | None:intergenic | 25.0% |
| ! | ACACATTCACTTACAATATA+TGG | - | chr6_3:18103487-18103506 | None:intergenic | 25.0% |
| ACAAAGAACAAATTGAACCA+TGG | + | chr6_3:18102227-18102246 | Msa0942760:exon | 30.0% | |
| CAAAGAACAAATTGAACCAT+GGG | + | chr6_3:18102228-18102247 | Msa0942760:exon | 30.0% | |
| AAAGAACAAATTGAACCATG+GGG | + | chr6_3:18102229-18102248 | Msa0942760:exon | 30.0% | |
| ! | GTGCAAGAACATAATTTTCT+TGG | + | chr6_3:18102251-18102270 | Msa0942760:CDS | 30.0% |
| ! | ATATTGCTACAATTGAGTTG+TGG | - | chr6_3:18102642-18102661 | None:intergenic | 30.0% |
| ACAACTCAATTGTAGCAATA+TGG | + | chr6_3:18102641-18102660 | Msa0942760:intron | 30.0% | |
| ! | AACTCAACTAGTTTGAACTT+AGG | + | chr6_3:18102670-18102689 | Msa0942760:intron | 30.0% |
| ! | ACTCAACTAGTTTGAACTTA+GGG | + | chr6_3:18102671-18102690 | Msa0942760:intron | 30.0% |
| ACGTCTATCCAAAAAAATTG+TGG | + | chr6_3:18102762-18102781 | Msa0942760:intron | 30.0% | |
| TGGAAAAGATGTTGTGAAAT+TGG | + | chr6_3:18103036-18103055 | Msa0942760:CDS | 30.0% | |
| TACATATACATACACTAGAG+AGG | + | chr6_3:18103353-18103372 | Msa0942760:three_prime_UTR | 30.0% | |
| ! | CATATTTGCTGAATATGAAG+AGG | - | chr6_3:18103405-18103424 | None:intergenic | 30.0% |
| ACAAAGAACAAATTGAACCA+TGG | + | chr6_3:18102227-18102246 | Msa0942760:exon | 30.0% | |
| CAAAGAACAAATTGAACCAT+GGG | + | chr6_3:18102228-18102247 | Msa0942760:exon | 30.0% | |
| AAAGAACAAATTGAACCATG+GGG | + | chr6_3:18102229-18102248 | Msa0942760:exon | 30.0% | |
| ! | GTGCAAGAACATAATTTTCT+TGG | + | chr6_3:18102251-18102270 | Msa0942760:CDS | 30.0% |
| ! | ATATTGCTACAATTGAGTTG+TGG | - | chr6_3:18102642-18102661 | None:intergenic | 30.0% |
| ACAACTCAATTGTAGCAATA+TGG | + | chr6_3:18102641-18102660 | Msa0942760:intron | 30.0% | |
| ! | AACTCAACTAGTTTGAACTT+AGG | + | chr6_3:18102670-18102689 | Msa0942760:intron | 30.0% |
| ! | ACTCAACTAGTTTGAACTTA+GGG | + | chr6_3:18102671-18102690 | Msa0942760:intron | 30.0% |
| ACGTCTATCCAAAAAAATTG+TGG | + | chr6_3:18102762-18102781 | Msa0942760:intron | 30.0% | |
| TGGAAAAGATGTTGTGAAAT+TGG | + | chr6_3:18103036-18103055 | Msa0942760:CDS | 30.0% | |
| TACATATACATACACTAGAG+AGG | + | chr6_3:18103353-18103372 | Msa0942760:three_prime_UTR | 30.0% | |
| ! | CATATTTGCTGAATATGAAG+AGG | - | chr6_3:18103405-18103424 | None:intergenic | 30.0% |
| TTGGCAACACAACATAATGT+TGG | + | chr6_3:18102306-18102325 | Msa0942760:CDS | 35.0% | |
| TCAGGTCAAACATTCAAAGT+TGG | + | chr6_3:18102375-18102394 | Msa0942760:CDS | 35.0% | |
| TTCAAAGTTGGAGATCAACT+TGG | + | chr6_3:18102387-18102406 | Msa0942760:CDS | 35.0% | |
| !! | TTGAGTTGAAACTCCGATTA+AGG | + | chr6_3:18102712-18102731 | Msa0942760:intron | 35.0% |
| ! | AACATCTTTTCCAGTGCTTA+AGG | - | chr6_3:18103029-18103048 | None:intergenic | 35.0% |
| ATGAAGGTAAAGATCACAGT+AGG | + | chr6_3:18103115-18103134 | Msa0942760:CDS | 35.0% | |
| GTAAAGATCACAGTAGGAAA+TGG | + | chr6_3:18103121-18103140 | Msa0942760:CDS | 35.0% | |
| TGAAGATGTATCAGATGTAG+GGG | - | chr6_3:18103221-18103240 | None:intergenic | 35.0% | |
| CTGAAGATGTATCAGATGTA+GGG | - | chr6_3:18103222-18103241 | None:intergenic | 35.0% | |
| !! | AAGAGAAGCAAAGTATTGTG+AGG | - | chr6_3:18103245-18103264 | None:intergenic | 35.0% |
| TTGGCAACACAACATAATGT+TGG | + | chr6_3:18102306-18102325 | Msa0942760:CDS | 35.0% | |
| TCAGGTCAAACATTCAAAGT+TGG | + | chr6_3:18102375-18102394 | Msa0942760:CDS | 35.0% | |
| TTCAAAGTTGGAGATCAACT+TGG | + | chr6_3:18102387-18102406 | Msa0942760:CDS | 35.0% | |
| !! | TTGAGTTGAAACTCCGATTA+AGG | + | chr6_3:18102712-18102731 | Msa0942760:intron | 35.0% |
| ! | AACATCTTTTCCAGTGCTTA+AGG | - | chr6_3:18103029-18103048 | None:intergenic | 35.0% |
| ATGAAGGTAAAGATCACAGT+AGG | + | chr6_3:18103115-18103134 | Msa0942760:CDS | 35.0% | |
| GTAAAGATCACAGTAGGAAA+TGG | + | chr6_3:18103121-18103140 | Msa0942760:CDS | 35.0% | |
| TGAAGATGTATCAGATGTAG+GGG | - | chr6_3:18103221-18103240 | None:intergenic | 35.0% | |
| CTGAAGATGTATCAGATGTA+GGG | - | chr6_3:18103222-18103241 | None:intergenic | 35.0% | |
| !! | AAGAGAAGCAAAGTATTGTG+AGG | - | chr6_3:18103245-18103264 | None:intergenic | 35.0% |
| AATTATGTTCTTGCACCCCA+TGG | - | chr6_3:18102247-18102266 | None:intergenic | 40.0% | |
| GTTAGCTACCTTGATCACAA+AGG | + | chr6_3:18102278-18102297 | Msa0942760:CDS | 40.0% | |
| CAATACCTCCTTTGTGATCA+AGG | - | chr6_3:18102289-18102308 | None:intergenic | 40.0% | |
| ! | CTTGATCACAAAGGAGGTAT+TGG | + | chr6_3:18102287-18102306 | Msa0942760:CDS | 40.0% |
| GCAACACAACATAATGTTGG+TGG | + | chr6_3:18102309-18102328 | Msa0942760:CDS | 40.0% | |
| ! | CATAATGTTGGTGGAAGTCA+AGG | + | chr6_3:18102318-18102337 | Msa0942760:CDS | 40.0% |
| CATGAATCAAAGTCTGAGGA+TGG | - | chr6_3:18102351-18102370 | None:intergenic | 40.0% | |
| CATCCTCAGACTTTGATTCA+TGG | + | chr6_3:18102349-18102368 | Msa0942760:CDS | 40.0% | |
| ACTCCATGAATCAAAGTCTG+AGG | - | chr6_3:18102355-18102374 | None:intergenic | 40.0% | |
| GACTTTGATTCATGGAGTTC+AGG | + | chr6_3:18102357-18102376 | Msa0942760:CDS | 40.0% | |
| GTACACATCTATGCATAGTG+TGG | + | chr6_3:18102946-18102965 | Msa0942760:CDS | 40.0% | |
| AGTGCTTAAGGAGTTTAGTG+GGG | - | chr6_3:18103017-18103036 | None:intergenic | 40.0% | |
| CAGTGCTTAAGGAGTTTAGT+GGG | - | chr6_3:18103018-18103037 | None:intergenic | 40.0% | |
| TACTTCACATGTGGTACCTT+AGG | + | chr6_3:18103076-18103095 | Msa0942760:CDS | 40.0% | |
| TCATTGTGGTCAAGGTATGA+AGG | + | chr6_3:18103099-18103118 | Msa0942760:CDS | 40.0% | |
| GCTGAAGATGTATCAGATGT+AGG | - | chr6_3:18103223-18103242 | None:intergenic | 40.0% | |
| AATTATGTTCTTGCACCCCA+TGG | - | chr6_3:18102247-18102266 | None:intergenic | 40.0% | |
| GTTAGCTACCTTGATCACAA+AGG | + | chr6_3:18102278-18102297 | Msa0942760:CDS | 40.0% | |
| CAATACCTCCTTTGTGATCA+AGG | - | chr6_3:18102289-18102308 | None:intergenic | 40.0% | |
| ! | CTTGATCACAAAGGAGGTAT+TGG | + | chr6_3:18102287-18102306 | Msa0942760:CDS | 40.0% |
| GCAACACAACATAATGTTGG+TGG | + | chr6_3:18102309-18102328 | Msa0942760:CDS | 40.0% | |
| ! | CATAATGTTGGTGGAAGTCA+AGG | + | chr6_3:18102318-18102337 | Msa0942760:CDS | 40.0% |
| CATGAATCAAAGTCTGAGGA+TGG | - | chr6_3:18102351-18102370 | None:intergenic | 40.0% | |
| CATCCTCAGACTTTGATTCA+TGG | + | chr6_3:18102349-18102368 | Msa0942760:CDS | 40.0% | |
| ACTCCATGAATCAAAGTCTG+AGG | - | chr6_3:18102355-18102374 | None:intergenic | 40.0% | |
| GACTTTGATTCATGGAGTTC+AGG | + | chr6_3:18102357-18102376 | Msa0942760:CDS | 40.0% | |
| GTACACATCTATGCATAGTG+TGG | + | chr6_3:18102946-18102965 | Msa0942760:CDS | 40.0% | |
| AGTGCTTAAGGAGTTTAGTG+GGG | - | chr6_3:18103017-18103036 | None:intergenic | 40.0% | |
| CAGTGCTTAAGGAGTTTAGT+GGG | - | chr6_3:18103018-18103037 | None:intergenic | 40.0% | |
| TACTTCACATGTGGTACCTT+AGG | + | chr6_3:18103076-18103095 | Msa0942760:CDS | 40.0% | |
| TCATTGTGGTCAAGGTATGA+AGG | + | chr6_3:18103099-18103118 | Msa0942760:CDS | 40.0% | |
| GCTGAAGATGTATCAGATGT+AGG | - | chr6_3:18103223-18103242 | None:intergenic | 40.0% | |
| AGCTACCTTGATCACAAAGG+AGG | + | chr6_3:18102281-18102300 | Msa0942760:CDS | 45.0% | |
| ! | ATGTTGGTGGAAGTCAAGGT+TGG | + | chr6_3:18102322-18102341 | Msa0942760:CDS | 45.0% |
| ! | TGTTGGTGGAAGTCAAGGTT+GGG | + | chr6_3:18102323-18102342 | Msa0942760:CDS | 45.0% |
| !!! | CGGCACTGCCACAATTTTTT+TGG | - | chr6_3:18102773-18102792 | None:intergenic | 45.0% |
| CCAGTGCTTAAGGAGTTTAG+TGG | - | chr6_3:18103019-18103038 | None:intergenic | 45.0% | |
| CCACTAAACTCCTTAAGCAC+TGG | + | chr6_3:18103016-18103035 | Msa0942760:CDS | 45.0% | |
| GTTGTGAAATTGGACAAGCC+AGG | + | chr6_3:18103046-18103065 | Msa0942760:CDS | 45.0% | |
| AATTGGACAAGCCAGGTACA+AGG | + | chr6_3:18103053-18103072 | Msa0942760:CDS | 45.0% | |
| ! | CATGTGAAGTACCTTGTACC+TGG | - | chr6_3:18103067-18103086 | None:intergenic | 45.0% |
| ! | GGTACAAGGTACTTCACATG+TGG | + | chr6_3:18103067-18103086 | Msa0942760:CDS | 45.0% |
| TGTGGTACCTTAGGTCATTG+TGG | + | chr6_3:18103085-18103104 | Msa0942760:CDS | 45.0% | |
| ACCTTAGGTCATTGTGGTCA+AGG | + | chr6_3:18103091-18103110 | Msa0942760:CDS | 45.0% | |
| ACCTTGACCACAATGACCTA+AGG | - | chr6_3:18103095-18103114 | None:intergenic | 45.0% | |
| ! | ATGGTGATGATGAAGACGAC+GGG | - | chr6_3:18103168-18103187 | None:intergenic | 45.0% |
| ! | GATGGTGATGATGAAGACGA+CGG | - | chr6_3:18103169-18103188 | None:intergenic | 45.0% |
| ! | CATACACTAGAGAGGTACAG+TGG | + | chr6_3:18103361-18103380 | Msa0942760:three_prime_UTR | 45.0% |
| ! | ACTAGAGAGGTACAGTGGAA+TGG | + | chr6_3:18103366-18103385 | Msa0942760:three_prime_UTR | 45.0% |
| AGCTACCTTGATCACAAAGG+AGG | + | chr6_3:18102281-18102300 | Msa0942760:CDS | 45.0% | |
| ! | ATGTTGGTGGAAGTCAAGGT+TGG | + | chr6_3:18102322-18102341 | Msa0942760:CDS | 45.0% |
| ! | TGTTGGTGGAAGTCAAGGTT+GGG | + | chr6_3:18102323-18102342 | Msa0942760:CDS | 45.0% |
| !!! | CGGCACTGCCACAATTTTTT+TGG | - | chr6_3:18102773-18102792 | None:intergenic | 45.0% |
| CCAGTGCTTAAGGAGTTTAG+TGG | - | chr6_3:18103019-18103038 | None:intergenic | 45.0% | |
| CCACTAAACTCCTTAAGCAC+TGG | + | chr6_3:18103016-18103035 | Msa0942760:CDS | 45.0% | |
| GTTGTGAAATTGGACAAGCC+AGG | + | chr6_3:18103046-18103065 | Msa0942760:CDS | 45.0% | |
| AATTGGACAAGCCAGGTACA+AGG | + | chr6_3:18103053-18103072 | Msa0942760:CDS | 45.0% | |
| ! | CATGTGAAGTACCTTGTACC+TGG | - | chr6_3:18103067-18103086 | None:intergenic | 45.0% |
| ! | GGTACAAGGTACTTCACATG+TGG | + | chr6_3:18103067-18103086 | Msa0942760:CDS | 45.0% |
| TGTGGTACCTTAGGTCATTG+TGG | + | chr6_3:18103085-18103104 | Msa0942760:CDS | 45.0% | |
| ACCTTAGGTCATTGTGGTCA+AGG | + | chr6_3:18103091-18103110 | Msa0942760:CDS | 45.0% | |
| ACCTTGACCACAATGACCTA+AGG | - | chr6_3:18103095-18103114 | None:intergenic | 45.0% | |
| ! | ATGGTGATGATGAAGACGAC+GGG | - | chr6_3:18103168-18103187 | None:intergenic | 45.0% |
| ! | GATGGTGATGATGAAGACGA+CGG | - | chr6_3:18103169-18103188 | None:intergenic | 45.0% |
| ! | CATACACTAGAGAGGTACAG+TGG | + | chr6_3:18103361-18103380 | Msa0942760:three_prime_UTR | 45.0% |
| ! | ACTAGAGAGGTACAGTGGAA+TGG | + | chr6_3:18103366-18103385 | Msa0942760:three_prime_UTR | 45.0% |
| ! | TGGTGATGATGAAGACGACG+GGG | - | chr6_3:18103167-18103186 | None:intergenic | 50.0% |
| ! | TGGTGATGATGAAGACGACG+GGG | - | chr6_3:18103167-18103186 | None:intergenic | 50.0% |
| ! | TGATGATGAAGACGACGGGG+AGG | - | chr6_3:18103164-18103183 | None:intergenic | 55.0% |
| ! | TGATGATGAAGACGACGGGG+AGG | - | chr6_3:18103164-18103183 | None:intergenic | 55.0% |
| GATGACGACGACGACGACGA+TGG | - | chr6_3:18103187-18103206 | None:intergenic | 60.0% | |
| GATGACGACGACGACGACGA+TGG | - | chr6_3:18103187-18103206 | None:intergenic | 60.0% | |
| CGGGGAGGATGCAGAGGAAG+AGG | - | chr6_3:18103149-18103168 | None:intergenic | 65.0% | |
| AGACGACGGGGAGGATGCAG+AGG | - | chr6_3:18103155-18103174 | None:intergenic | 65.0% | |
| CGGGGAGGATGCAGAGGAAG+AGG | - | chr6_3:18103149-18103168 | None:intergenic | 65.0% | |
| AGACGACGGGGAGGATGCAG+AGG | - | chr6_3:18103155-18103174 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 18102181 | 18103579 | 18102181 | ID=Msa0942760;Name=Msa0942760 |
| chr6_3 | mRNA | 18102181 | 18103579 | 18102181 | ID=Msa0942760-mRNA-1;Parent=Msa0942760;Name=Msa0942760-mRNA-1;_AED=0.00;_eAED=0.00;_QI=65|1|1|1|1|1|2|267|178 |
| chr6_3 | exon | 18102181 | 18102408 | 18102181 | ID=Msa0942760-mRNA-1:exon:5400;Parent=Msa0942760-mRNA-1 |
| chr6_3 | exon | 18102939 | 18103579 | 18102939 | ID=Msa0942760-mRNA-1:exon:5401;Parent=Msa0942760-mRNA-1 |
| chr6_3 | five_prime_UTR | 18102181 | 18102245 | 18102181 | ID=Msa0942760-mRNA-1:five_prime_utr;Parent=Msa0942760-mRNA-1 |
| chr6_3 | CDS | 18102246 | 18102408 | 18102246 | ID=Msa0942760-mRNA-1:cds;Parent=Msa0942760-mRNA-1 |
| chr6_3 | CDS | 18102939 | 18103312 | 18102939 | ID=Msa0942760-mRNA-1:cds;Parent=Msa0942760-mRNA-1 |
| chr6_3 | three_prime_UTR | 18103313 | 18103579 | 18103313 | ID=Msa0942760-mRNA-1:three_prime_utr;Parent=Msa0942760-mRNA-1 |
| Gene Sequence |
| Protein sequence |
