AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0952140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0952140 MtrunA17_Chr6g0457151 48.630 146 18 3 35 123 111 256 3.23e-30 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0952140 AT5G38660.1 43.151 146 26 3 35 123 107 252 8.81e-26 100
Msa0952140 AT5G38660.2 39.634 164 42 3 35 141 107 270 1.91e-25 101

Find 65 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGGGTTTAATAACAATTT+TGG 0.060975 6_3:+45442584 None:intergenic
TTCTCGTTCATTTAATTAAA+AGG 0.122256 6_3:-45442185 Msa0952140:five_prime_UTR
AATGCGATTTGGAGCGTTTA+AGG 0.179410 6_3:+45442566 None:intergenic
TGAAAAGTTATGAGCTTATA+TGG 0.212630 6_3:-45442240 Msa0952140:five_prime_UTR
GGTTTAGGAACAACACAAAT+TGG 0.234037 6_3:+45442621 None:intergenic
TGCTTTATGTTTCAGGGAAA+TGG 0.263031 6_3:-45440177 Msa0952140:intron
GAAATCACGTTTGTGTGTTT+TGG 0.268769 6_3:+45442768 None:intergenic
TAAATACACAGCTATGGTTT+TGG 0.282611 6_3:-45441229 Msa0952140:intron
CTCTTGCAATGGAGGAATTC+AGG 0.306076 6_3:-45439789 Msa0952140:CDS
AGAATGGGTGCACTTCTTCT+AGG 0.314080 6_3:+45440150 None:intergenic
ATCTAAACCATAAAAGAAAA+AGG 0.327781 6_3:+45442445 None:intergenic
TGTTCTATTTCTTCTCAAAC+TGG 0.355330 6_3:-45442493 Msa0952140:five_prime_UTR
CTTACCTCTTCATGAGCATA+AGG 0.356653 6_3:-45439830 Msa0952140:CDS
TGGAAAATATTGTTTGGTCT+TGG 0.379387 6_3:-45439959 Msa0952140:CDS
TTTCAAACATTAGATGCATA+TGG 0.382951 6_3:-45441941 Msa0952140:CDS
CAATCGGCGAGAATGCGATT+TGG 0.391598 6_3:+45442555 None:intergenic
GGTTTCCCTCTTGCGATCAT+TGG 0.394311 6_3:-45441151 Msa0952140:CDS
TATGAGCATGCTTATTTATG+AGG 0.394684 6_3:-45441994 Msa0952140:five_prime_UTR
TTTCATGGTTTGAGAGGAAA+GGG 0.412422 6_3:+45442745 None:intergenic
ATGCGATTTGGAGCGTTTAA+GGG 0.415777 6_3:+45442567 None:intergenic
TTCACGAACCATTTGTAGAA+TGG 0.424557 6_3:+45440134 None:intergenic
TCTCGTTCATTTAATTAAAA+GGG 0.425959 6_3:-45442184 Msa0952140:five_prime_UTR
GTTTAGGAACAACACAAATT+GGG 0.431832 6_3:+45442622 None:intergenic
ACAAGATGGAAAATATTGTT+TGG 0.433342 6_3:-45439965 Msa0952140:CDS
AAAGCTTCACAGCAGATTTG+AGG 0.451804 6_3:+45442520 None:intergenic
TCAGCTATAATGTTAACATA+TGG 0.454586 6_3:-45441172 Msa0952140:CDS
CCCTCTTGCGATCATTGGCA+TGG 0.456323 6_3:-45441146 Msa0952140:CDS
AGAATTGATTCAGAGTTTCA+TGG 0.457026 6_3:+45442730 None:intergenic
TCACGAACCATTTGTAGAAT+GGG 0.457217 6_3:+45440135 None:intergenic
GAGGAATCGAAGGCAGCAAT+CGG 0.475493 6_3:+45442539 None:intergenic
GAATGGGTGCACTTCTTCTA+GGG 0.489478 6_3:+45440151 None:intergenic
AAGTGCACCCATTCTACAAA+TGG 0.495758 6_3:-45440142 Msa0952140:CDS
AGCATACATTAATATTCTCC+CGG 0.500422 6_3:-45441206 Msa0952140:CDS
TTGTTTGTGAATGGGTTTGG+TGG 0.501523 6_3:-45442057 Msa0952140:five_prime_UTR
TAACATTATAGCTGACCATT+CGG 0.507124 6_3:+45441180 None:intergenic
ATAGCTGACCATTCGGATCC+CGG 0.513087 6_3:+45441187 None:intergenic
GAAGCTTTAGAGAATCAATC+AGG 0.514653 6_3:-45439757 Msa0952140:CDS
TAATTATATCACTCTTGCAA+TGG 0.525678 6_3:-45439800 Msa0952140:CDS
TCAGAGTTTCATGGTTTGAG+AGG 0.530265 6_3:+45442739 None:intergenic
TTATATCACTCTTGCAATGG+AGG 0.531910 6_3:-45439797 Msa0952140:CDS
TAGCTGACCATTCGGATCCC+GGG 0.532144 6_3:+45441188 None:intergenic
GTTTCATGGTTTGAGAGGAA+AGG 0.533230 6_3:+45442744 None:intergenic
TGAGAAGTGATTACGAGTGA+TGG 0.538683 6_3:+45442668 None:intergenic
GATCATTGGCATGGCACTCA+AGG 0.551544 6_3:-45441137 Msa0952140:intron
ACATTAGATGCATATGGAGA+TGG 0.555943 6_3:-45441935 Msa0952140:CDS
GCTAGGCAGTTCAAGTACTC+TGG 0.556030 6_3:-45441910 Msa0952140:CDS
TCTGATTGATCATCAAGAGC+AGG 0.560143 6_3:+45439679 None:intergenic
GAGAAGTGATTACGAGTGAT+GGG 0.563607 6_3:+45442669 None:intergenic
TCTACAAATGGTTCGTGAAG+AGG 0.564336 6_3:-45440130 Msa0952140:intron
TACCTCAAGAGGCAATCTCT+AGG 0.570502 6_3:+45441858 None:intergenic
CAGCAGATTTGAGGAATCGA+AGG 0.575247 6_3:+45442529 None:intergenic
GTTTCCTTATGCTCATGAAG+AGG 0.577885 6_3:+45439826 None:intergenic
ACCAAAGGGCTTACCTCAAG+AGG 0.578707 6_3:+45441847 None:intergenic
CACCTAGAGATTGCCTCTTG+AGG 0.579526 6_3:-45441860 Msa0952140:intron
TTAGAGAATCAATCAGGCAG+TGG 0.586617 6_3:-45439751 Msa0952140:CDS
TGCTCTTGATGATCAATCAG+AGG 0.598343 6_3:-45439677 Msa0952140:CDS
GGAGGAATTCAGGAAGCGCA+TGG 0.599565 6_3:-45439779 Msa0952140:CDS
GCATACATTAATATTCTCCC+GGG 0.600186 6_3:-45441205 Msa0952140:CDS
TTCATGGTTTGAGAGGAAAG+GGG 0.622906 6_3:+45442746 None:intergenic
TGCATATGGAGATGGATGCT+AGG 0.625604 6_3:-45441927 Msa0952140:CDS
GCCATGCCAATGATCGCAAG+AGG 0.627559 6_3:+45441145 None:intergenic
ATATTCTCCCGGGATCCGAA+TGG 0.638716 6_3:-45441195 Msa0952140:CDS
TCAAGAGCAGGATCATAATG+TGG 0.646262 6_3:+45439691 None:intergenic
CCATGCCAATGATCGCAAGA+GGG 0.652724 6_3:+45441146 None:intergenic
ACACCAACAAACATTAGGTG+AGG 0.663720 6_3:+45439650 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATATTTTGTTTATTGTT+TGG - chr6_3:45440074-45440093 Msa0952140:intron 10.0%
!! TCAATAAAAAAAAATAATTG+AGG + chr6_3:45441936-45441955 None:intergenic 10.0%
!!! GTGTATTTAAAACAAATAAA+AGG + chr6_3:45441211-45441230 None:intergenic 15.0%
!! TTAATGTTAATCAAGTTAAA+AGG - chr6_3:45441352-45441371 Msa0952140:intron 15.0%
!! AAGTAATTAAAAATGTATTC+AGG + chr6_3:45442582-45442601 None:intergenic 15.0%
!!! AAGGGTTTAATAACAATTTT+GGG + chr6_3:45439869-45439888 None:intergenic 20.0%
!!! TAAGGGTTTAATAACAATTT+TGG + chr6_3:45439870-45439889 None:intergenic 20.0%
!! ATCTAAACCATAAAAGAAAA+AGG + chr6_3:45440009-45440028 None:intergenic 20.0%
!! TTCTCGTTCATTTAATTAAA+AGG - chr6_3:45440266-45440285 Msa0952140:intron 20.0%
!! TCTCGTTCATTTAATTAAAA+GGG - chr6_3:45440267-45440286 Msa0952140:intron 20.0%
!!! TTGATATTTTTGTTTGTGTT+TGG - chr6_3:45440767-45440786 Msa0952140:intron 20.0%
!! ATAAAATTTCATAACTAGTC+AGG - chr6_3:45441052-45441071 Msa0952140:intron 20.0%
!!! TAAATTTTGCAGATTTCATT+TGG - chr6_3:45441082-45441101 Msa0952140:intron 20.0%
!! ATAAAAGGAAAACAAACATA+AGG + chr6_3:45441196-45441215 None:intergenic 20.0%
!! TAAATCTATATCTTTAGTGT+TGG + chr6_3:45441665-45441684 None:intergenic 20.0%
!! AGAAGAAAGATTGAATATAT+TGG + chr6_3:45441775-45441794 None:intergenic 20.0%
!!! TAATAATATGCCTATGTTTT+AGG - chr6_3:45442459-45442478 Msa0952140:five_prime_UTR 20.0%
!!! ATTTTGAGAAGTGATTTTGA+GGG + chr6_3:45439749-45439768 None:intergenic 25.0%
!!! ATTTTGGGATTGATTTTCTT+AGG + chr6_3:45439854-45439873 None:intergenic 25.0%
! AAACCATAAAAGAAAAAGGA+CGG + chr6_3:45440005-45440024 None:intergenic 25.0%
! TGAAAAGTTATGAGCTTATA+TGG - chr6_3:45440211-45440230 Msa0952140:intron 25.0%
!!! AGGCATTTTGTTTGATATTT+TGG - chr6_3:45440365-45440384 Msa0952140:intron 25.0%
!!! GGCATTTTGTTTGATATTTT+GGG - chr6_3:45440366-45440385 Msa0952140:intron 25.0%
!!! GCATTTTGTTTGATATTTTG+GGG - chr6_3:45440367-45440386 Msa0952140:intron 25.0%
! TTTCAAACATTAGATGCATA+TGG - chr6_3:45440510-45440529 Msa0952140:intron 25.0%
!!! GATTAGCTTAGGAAATAAAT+CGG + chr6_3:45440911-45440930 None:intergenic 25.0%
! CTATAATGATATATCGATGA+AGG + chr6_3:45441166-45441185 None:intergenic 25.0%
!!! TTGTTTTAAATACACAGCTA+TGG - chr6_3:45441216-45441235 Msa0952140:CDS 25.0%
! TCAGCTATAATGTTAACATA+TGG - chr6_3:45441279-45441298 Msa0952140:intron 25.0%
! ATGTTAATCAAGTTAAAAGG+TGG - chr6_3:45441355-45441374 Msa0952140:intron 25.0%
! TTTCTTCTAATCACTTAAAG+AGG - chr6_3:45441787-45441806 Msa0952140:intron 25.0%
! TATAACTTACTAGACCATAT+TGG + chr6_3:45441903-45441922 None:intergenic 25.0%
! TTATTAAAGCTTACCTACTA+GGG + chr6_3:45442115-45442134 None:intergenic 25.0%
! TATTATCTGCTTTATGTTTC+AGG - chr6_3:45442267-45442286 Msa0952140:five_prime_UTR 25.0%
! ATTATCTGCTTTATGTTTCA+GGG - chr6_3:45442268-45442287 Msa0952140:five_prime_UTR 25.0%
! AAACATAGGCATATTATTAG+AGG + chr6_3:45442458-45442477 None:intergenic 25.0%
! ACAAGATGGAAAATATTGTT+TGG - chr6_3:45442486-45442505 Msa0952140:five_prime_UTR 25.0%
! TAATTATATCACTCTTGCAA+TGG - chr6_3:45442651-45442670 Msa0952140:five_prime_UTR 25.0%
! AGAATTGATTCAGAGTTTCA+TGG + chr6_3:45439724-45439743 None:intergenic 30.0%
!! GATTTTGAGAAGTGATTTTG+AGG + chr6_3:45439750-45439769 None:intergenic 30.0%
! CAACACAAATTGGGAATTTT+AGG + chr6_3:45439823-45439842 None:intergenic 30.0%
GTTTAGGAACAACACAAATT+GGG + chr6_3:45439832-45439851 None:intergenic 30.0%
!! GGATTGATTTTCTTAGGTTT+AGG + chr6_3:45439848-45439867 None:intergenic 30.0%
! TGTTCTATTTCTTCTCAAAC+TGG - chr6_3:45439958-45439977 Msa0952140:CDS 30.0%
! GATTTGTGAAGATGTTTTAC+TGG - chr6_3:45440026-45440045 Msa0952140:intron 30.0%
!! ATTTTGTTTGTGAATGGGTT+TGG - chr6_3:45440391-45440410 Msa0952140:intron 30.0%
TATGAGCATGCTTATTTATG+AGG - chr6_3:45440457-45440476 Msa0952140:intron 30.0%
!! AATACACACAAGATTAGCTT+AGG + chr6_3:45440922-45440941 None:intergenic 30.0%
! AATTTTATGCAAAGACAGCA+AGG + chr6_3:45440984-45441003 None:intergenic 30.0%
CAGATTTCATTTGGTACAAT+TGG - chr6_3:45441091-45441110 Msa0952140:intron 30.0%
! TAAATACACAGCTATGGTTT+TGG - chr6_3:45441222-45441241 Msa0952140:intron 30.0%
AGCATACATTAATATTCTCC+CGG - chr6_3:45441245-45441264 Msa0952140:intron 30.0%
TAACATTATAGCTGACCATT+CGG + chr6_3:45441274-45441293 None:intergenic 30.0%
! ATATTGTGACACTCTTTTCT+TGG - chr6_3:45441694-45441713 Msa0952140:intron 30.0%
TGGGGATGAATAACATTTAT+AGG - chr6_3:45441858-45441877 Msa0952140:intron 30.0%
GGATGAATAACATTTATAGG+AGG - chr6_3:45441861-45441880 Msa0952140:CDS 30.0%
!! TTGAAACGGATTTTCCAATA+TGG - chr6_3:45441886-45441905 Msa0952140:CDS 30.0%
ACTTATTAGAGCTTATTTGC+TGG + chr6_3:45441992-45442011 None:intergenic 30.0%
ATTCATCTTAAAGGTCTGAT+TGG + chr6_3:45442019-45442038 None:intergenic 30.0%
!! ATCAGACCTTTAAGATGAAT+TGG - chr6_3:45442019-45442038 Msa0952140:five_prime_UTR 30.0%
CTAGAACCAATTCATCTTAA+AGG + chr6_3:45442028-45442047 None:intergenic 30.0%
CTTATTAAAGCTTACCTACT+AGG + chr6_3:45442116-45442135 None:intergenic 30.0%
TCCTAAAGGTCTATTTGAAT+TGG + chr6_3:45442139-45442158 None:intergenic 30.0%
GAAGATATCAGAAAGTAGTA+AGG + chr6_3:45442180-45442199 None:intergenic 30.0%
GTCAACATTAGAGATAGATT+TGG - chr6_3:45442378-45442397 Msa0952140:five_prime_UTR 30.0%
! AAGTCTTATCCAACTAAGTT+GGG + chr6_3:45442414-45442433 None:intergenic 30.0%
TGGAAAATATTGTTTGGTCT+TGG - chr6_3:45442492-45442511 Msa0952140:five_prime_UTR 30.0%
! AAAGTTGTCAGATTTTGAAC+AGG - chr6_3:45442534-45442553 Msa0952140:five_prime_UTR 30.0%
! AAGTTGTCAGATTTTGAACA+GGG - chr6_3:45442535-45442554 Msa0952140:five_prime_UTR 30.0%
! GAAATCACGTTTGTGTGTTT+TGG + chr6_3:45439686-45439705 None:intergenic 35.0%
TTTCATGGTTTGAGAGGAAA+GGG + chr6_3:45439709-45439728 None:intergenic 35.0%
GGTTTAGGAACAACACAAAT+TGG + chr6_3:45439833-45439852 None:intergenic 35.0%
! CAACCGTCCTTTTTCTTTTA+TGG - chr6_3:45439999-45440018 Msa0952140:intron 35.0%
ACATTAGATGCATATGGAGA+TGG - chr6_3:45440516-45440535 Msa0952140:intron 35.0%
!!! CTTATTTTACGAGTGGAGTT+GGG - chr6_3:45440568-45440587 Msa0952140:intron 35.0%
!! TTTTTGTTTGTGTTTGGCTG+TGG - chr6_3:45440773-45440792 Msa0952140:intron 35.0%
!! TTTTGTTTGTGTTTGGCTGT+GGG - chr6_3:45440774-45440793 Msa0952140:intron 35.0%
! TAAGCTATGGGTGTGAATTT+TGG - chr6_3:45440807-45440826 Msa0952140:intron 35.0%
GCATACATTAATATTCTCCC+GGG - chr6_3:45441246-45441265 Msa0952140:intron 35.0%
TTTGAGTGTACAAATCGTGT+CGG + chr6_3:45441512-45441531 None:intergenic 35.0%
! TTCTTTGTGGTTCTATGCAT+TGG - chr6_3:45441579-45441598 Msa0952140:intron 35.0%
! AGGCTCTTTTGTGCTTTTAT+AGG - chr6_3:45441807-45441826 Msa0952140:intron 35.0%
! CTTTTGTGCTTTTATAGGTG+AGG - chr6_3:45441812-45441831 Msa0952140:intron 35.0%
ATGTTACCAGATATCGCTAT+GGG - chr6_3:45441839-45441858 Msa0952140:intron 35.0%
!! ATTTATAGGAGGCGTTGAAA+CGG - chr6_3:45441872-45441891 Msa0952140:CDS 35.0%
!!! TAAGCACTTTTGAGACGATT+TGG + chr6_3:45441967-45441986 None:intergenic 35.0%
!! TAAGCACTTTTTAGACCATC+TGG + chr6_3:45442092-45442111 None:intergenic 35.0%
!! TAAAAAGTGCTTACCCTAGT+AGG - chr6_3:45442099-45442118 Msa0952140:five_prime_UTR 35.0%
GCCAATTCAAATAGACCTTT+AGG - chr6_3:45442135-45442154 Msa0952140:five_prime_UTR 35.0%
!! CAAATAGACCTTTAGGATGA+AGG - chr6_3:45442142-45442161 Msa0952140:five_prime_UTR 35.0%
TGCTTTATGTTTCAGGGAAA+TGG - chr6_3:45442274-45442293 Msa0952140:five_prime_UTR 35.0%
TCACGAACCATTTGTAGAAT+GGG + chr6_3:45442319-45442338 None:intergenic 35.0%
TTCACGAACCATTTGTAGAA+TGG + chr6_3:45442320-45442339 None:intergenic 35.0%
! GAAGTCTTATCCAACTAAGT+TGG + chr6_3:45442415-45442434 None:intergenic 35.0%
TCTTGTGTGACCTAAAACAT+AGG + chr6_3:45442472-45442491 None:intergenic 35.0%
!! ATGTTTTAGGTCACACAAGA+TGG - chr6_3:45442472-45442491 Msa0952140:five_prime_UTR 35.0%
TTATATCACTCTTGCAATGG+AGG - chr6_3:45442654-45442673 Msa0952140:five_prime_UTR 35.0%
!! GAAGCTTTAGAGAATCAATC+AGG - chr6_3:45442694-45442713 Msa0952140:five_prime_UTR 35.0%
TTCATGGTTTGAGAGGAAAG+GGG + chr6_3:45439708-45439727 None:intergenic 40.0%
GTTTCATGGTTTGAGAGGAA+AGG + chr6_3:45439710-45439729 None:intergenic 40.0%
TCAGAGTTTCATGGTTTGAG+AGG + chr6_3:45439715-45439734 None:intergenic 40.0%
GAGAAGTGATTACGAGTGAT+GGG + chr6_3:45439785-45439804 None:intergenic 40.0%
TGAGAAGTGATTACGAGTGA+TGG + chr6_3:45439786-45439805 None:intergenic 40.0%
! ATGCGATTTGGAGCGTTTAA+GGG + chr6_3:45439887-45439906 None:intergenic 40.0%
! AATGCGATTTGGAGCGTTTA+AGG + chr6_3:45439888-45439907 None:intergenic 40.0%
AAAGCTTCACAGCAGATTTG+AGG + chr6_3:45439934-45439953 None:intergenic 40.0%
!! TGGGGCATTTTGTTTGTGAA+TGG - chr6_3:45440385-45440404 Msa0952140:intron 40.0%
!! GGGGCATTTTGTTTGTGAAT+GGG - chr6_3:45440386-45440405 Msa0952140:intron 40.0%
! TTGTTTGTGAATGGGTTTGG+TGG - chr6_3:45440394-45440413 Msa0952140:intron 40.0%
!!! GCTTATTTTACGAGTGGAGT+TGG - chr6_3:45440567-45440586 Msa0952140:intron 40.0%
!!! AGTGTTTTCTGGGACTGTTT+TGG - chr6_3:45440724-45440743 Msa0952140:intron 40.0%
GGTACAATTGGACTAAGTCT+TGG - chr6_3:45441103-45441122 Msa0952140:intron 40.0%
TGTCATTGCCATACAAGTGT+CGG - chr6_3:45441483-45441502 Msa0952140:intron 40.0%
! GCATCGACACTTCAAATTGA+AGG + chr6_3:45441540-45441559 None:intergenic 40.0%
!!! GTGGTTCTATGCATTGGTTT+TGG - chr6_3:45441585-45441604 Msa0952140:intron 40.0%
!!! GTTTTGGCTGATACTCTTCT+GGG - chr6_3:45441601-45441620 Msa0952140:intron 40.0%
GATGTTACCAGATATCGCTA+TGG - chr6_3:45441838-45441857 Msa0952140:intron 40.0%
TGTTACCAGATATCGCTATG+GGG - chr6_3:45441840-45441859 Msa0952140:intron 40.0%
TTTCCATAAGCTCTTCCAGA+TGG - chr6_3:45442074-45442093 Msa0952140:five_prime_UTR 40.0%
! CTTTTGCTCCTTCATCCTAA+AGG + chr6_3:45442153-45442172 None:intergenic 40.0%
GCTGCATTATCTAGACACTA+AGG - chr6_3:45442206-45442225 Msa0952140:five_prime_UTR 40.0%
! ATTGCAACAAGAGCACATAG+AGG + chr6_3:45442235-45442254 None:intergenic 40.0%
AAGTGCACCCATTCTACAAA+TGG - chr6_3:45442309-45442328 Msa0952140:five_prime_UTR 40.0%
TCTACAAATGGTTCGTGAAG+AGG - chr6_3:45442321-45442340 Msa0952140:five_prime_UTR 40.0%
AATAGACGACCCAACTTAGT+TGG - chr6_3:45442402-45442421 Msa0952140:five_prime_UTR 40.0%
!!! TTGTTTGGTCTTGGTTTTCG+AGG - chr6_3:45442501-45442520 Msa0952140:five_prime_UTR 40.0%
CTTACCTCTTCATGAGCATA+AGG - chr6_3:45442621-45442640 Msa0952140:five_prime_UTR 40.0%
GTTTCCTTATGCTCATGAAG+AGG + chr6_3:45442628-45442647 None:intergenic 40.0%
TTAGAGAATCAATCAGGCAG+TGG - chr6_3:45442700-45442719 Msa0952140:five_prime_UTR 40.0%
TCAAGAGCAGGATCATAATG+TGG + chr6_3:45442763-45442782 None:intergenic 40.0%
TCTGATTGATCATCAAGAGC+AGG + chr6_3:45442775-45442794 None:intergenic 40.0%
! TGCTCTTGATGATCAATCAG+AGG - chr6_3:45442774-45442793 Msa0952140:five_prime_UTR 40.0%
CAGCAGATTTGAGGAATCGA+AGG + chr6_3:45439925-45439944 None:intergenic 45.0%
TGCATATGGAGATGGATGCT+AGG - chr6_3:45440524-45440543 Msa0952140:intron 45.0%
!!! TGGCGTGCTTATTTTACGAG+TGG - chr6_3:45440561-45440580 Msa0952140:intron 45.0%
TACCTCAAGAGGCAATCTCT+AGG + chr6_3:45440596-45440615 None:intergenic 45.0%
!!! TTTTCTGGGACTGTTTTGGC+AGG - chr6_3:45440728-45440747 Msa0952140:intron 45.0%
!!! TTTCTGGGACTGTTTTGGCA+GGG - chr6_3:45440729-45440748 Msa0952140:intron 45.0%
GATGCTACAATGTGTTCGTG+CGG - chr6_3:45441555-45441574 Msa0952140:intron 45.0%
!!! GGTTTTGGCTGATACTCTTC+TGG - chr6_3:45441600-45441619 Msa0952140:intron 45.0%
TGGTACAGGTAAAGAGTCCA+TGG + chr6_3:45441627-45441646 None:intergenic 45.0%
TTCATCCCCATAGCGATATC+TGG + chr6_3:45441848-45441867 None:intergenic 45.0%
AGACCATCTGGAAGAGCTTA+TGG + chr6_3:45442080-45442099 None:intergenic 45.0%
GAATGGGTGCACTTCTTCTA+GGG + chr6_3:45442303-45442322 None:intergenic 45.0%
AGAATGGGTGCACTTCTTCT+AGG + chr6_3:45442304-45442323 None:intergenic 45.0%
! GTCAGATTTTGAACAGGGAC+AGG - chr6_3:45442540-45442559 Msa0952140:five_prime_UTR 45.0%
CTCTTGCAATGGAGGAATTC+AGG - chr6_3:45442662-45442681 Msa0952140:five_prime_UTR 45.0%
!!! TTTTTTTTTTATCTATTTTA+AGG - chr6_3:45440345-45440364 Msa0952140:intron 5.0%
CAATCGGCGAGAATGCGATT+TGG + chr6_3:45439899-45439918 None:intergenic 50.0%
GAGGAATCGAAGGCAGCAAT+CGG + chr6_3:45439915-45439934 None:intergenic 50.0%
GCTAGGCAGTTCAAGTACTC+TGG - chr6_3:45440541-45440560 Msa0952140:intron 50.0%
CACCTAGAGATTGCCTCTTG+AGG - chr6_3:45440591-45440610 Msa0952140:intron 50.0%
ACCAAAGGGCTTACCTCAAG+AGG + chr6_3:45440607-45440626 None:intergenic 50.0%
CAGACTCGCGAAACACCAAA+GGG + chr6_3:45440621-45440640 None:intergenic 50.0%
ACAGACTCGCGAAACACCAA+AGG + chr6_3:45440622-45440641 None:intergenic 50.0%
! TTGGTGTTTCGCGAGTCTGT+CGG - chr6_3:45440622-45440641 Msa0952140:intron 50.0%
!! TTTCGCGAGTCTGTCGGTTT+GGG - chr6_3:45440628-45440647 Msa0952140:intron 50.0%
! CCTGCTGTGCAGTGTTTTCT+GGG - chr6_3:45440714-45440733 Msa0952140:intron 50.0%
!!! TTCTGGGACTGTTTTGGCAG+GGG - chr6_3:45440730-45440749 Msa0952140:intron 50.0%
TGTTTGTGTTTGGCTGTGGG+AGG - chr6_3:45440777-45440796 Msa0952140:intron 50.0%
ATATTCTCCCGGGATCCGAA+TGG - chr6_3:45441256-45441275 Msa0952140:intron 50.0%
ATAGCTGACCATTCGGATCC+CGG + chr6_3:45441267-45441286 None:intergenic 50.0%
GGTTTCCCTCTTGCGATCAT+TGG - chr6_3:45441300-45441319 Msa0952140:intron 50.0%
CCATGCCAATGATCGCAAGA+GGG + chr6_3:45441308-45441327 None:intergenic 50.0%
GATCATTGGCATGGCACTCA+AGG - chr6_3:45441314-45441333 Msa0952140:intron 50.0%
GTGTTCGTGCGGTTTCTTTG+TGG - chr6_3:45441566-45441585 Msa0952140:intron 50.0%
! CACTGAAGTGTCAGTGGTAC+AGG + chr6_3:45441641-45441660 None:intergenic 50.0%
! GTTGGACACTGAAGTGTCAG+TGG + chr6_3:45441647-45441666 None:intergenic 50.0%
!! GGTCTTGGTTTTCGAGGCTA+AGG - chr6_3:45442507-45442526 Msa0952140:five_prime_UTR 50.0%
GCCTCTTGAGGTAAGCCCTT+TGG - chr6_3:45440603-45440622 Msa0952140:intron 55.0%
! GTTTCGCGAGTCTGTCGGTT+TGG - chr6_3:45440627-45440646 Msa0952140:intron 55.0%
!! TTCGCGAGTCTGTCGGTTTG+GGG - chr6_3:45440629-45440648 Msa0952140:intron 55.0%
GCTCGGTTATGACTGCTGTC+AGG - chr6_3:45440661-45440680 Msa0952140:intron 55.0%
! GCCTGCTGTGCAGTGTTTTC+TGG - chr6_3:45440713-45440732 Msa0952140:intron 55.0%
CCCAGAAAACACTGCACAGC+AGG + chr6_3:45440717-45440736 None:intergenic 55.0%
TTGTGTTTGGCTGTGGGAGG+TGG - chr6_3:45440780-45440799 Msa0952140:intron 55.0%
TGTGTTTGGCTGTGGGAGGT+GGG - chr6_3:45440781-45440800 Msa0952140:intron 55.0%
TAGCTGACCATTCGGATCCC+GGG + chr6_3:45441266-45441285 None:intergenic 55.0%
CCCTCTTGCGATCATTGGCA+TGG - chr6_3:45441305-45441324 Msa0952140:intron 55.0%
GCCATGCCAATGATCGCAAG+AGG + chr6_3:45441309-45441328 None:intergenic 55.0%
GTCGGTCTCCGACACTTGTA+TGG + chr6_3:45441494-45441513 None:intergenic 55.0%
GCTGATACTCTTCTGGGCCA+TGG - chr6_3:45441607-45441626 Msa0952140:intron 55.0%
! GGAGGAATTCAGGAAGCGCA+TGG - chr6_3:45442672-45442691 Msa0952140:five_prime_UTR 55.0%
!! TCGCGAGTCTGTCGGTTTGG+GGG - chr6_3:45440630-45440649 Msa0952140:intron 60.0%
GTGTTTGGCTGTGGGAGGTG+GGG - chr6_3:45440782-45440801 Msa0952140:intron 60.0%
TGTTTGGCTGTGGGAGGTGG+GGG - chr6_3:45440783-45440802 Msa0952140:intron 60.0%
GGAGGTGGGGGCTAAGCTAT+GGG - chr6_3:45440795-45440814 Msa0952140:intron 60.0%
!! CGCGAGTCTGTCGGTTTGGG+GGG - chr6_3:45440631-45440650 Msa0952140:intron 65.0%
!! TGTCGGTTTGGGGGGTCTGC+TGG - chr6_3:45440639-45440658 Msa0952140:intron 65.0%
! GTTTGGGGGGTCTGCTGGCT+CGG - chr6_3:45440644-45440663 Msa0952140:intron 65.0%
GGGAGGTGGGGGCTAAGCTA+TGG - chr6_3:45440794-45440813 Msa0952140:intron 65.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 45439663 45442810 45439663 ID=Msa0952140;Name=Msa0952140
chr6_3 mRNA 45439663 45442810 45439663 ID=Msa0952140-mRNA-1;Parent=Msa0952140;Name=Msa0952140-mRNA-1;_AED=0.18;_eAED=0.18;_QI=840|0.25|0.8|1|0.75|1|5|0|184
chr6_3 exon 45439663 45439869 45439663 ID=Msa0952140-mRNA-1:exon:10195;Parent=Msa0952140-mRNA-1
chr6_3 exon 45439912 45439992 45439912 ID=Msa0952140-mRNA-1:exon:10194;Parent=Msa0952140-mRNA-1
chr6_3 exon 45440131 45440184 45440131 ID=Msa0952140-mRNA-1:exon:10193;Parent=Msa0952140-mRNA-1
chr6_3 exon 45441138 45441240 45441138 ID=Msa0952140-mRNA-1:exon:10192;Parent=Msa0952140-mRNA-1
chr6_3 exon 45441861 45442810 45441861 ID=Msa0952140-mRNA-1:exon:10191;Parent=Msa0952140-mRNA-1
chr6_3 five_prime_UTR 45441971 45442810 45441971 ID=Msa0952140-mRNA-1:five_prime_utr;Parent=Msa0952140-mRNA-1
chr6_3 CDS 45441861 45441970 45441861 ID=Msa0952140-mRNA-1:cds;Parent=Msa0952140-mRNA-1
chr6_3 CDS 45441138 45441240 45441138 ID=Msa0952140-mRNA-1:cds;Parent=Msa0952140-mRNA-1
chr6_3 CDS 45440131 45440184 45440131 ID=Msa0952140-mRNA-1:cds;Parent=Msa0952140-mRNA-1
chr6_3 CDS 45439912 45439992 45439912 ID=Msa0952140-mRNA-1:cds;Parent=Msa0952140-mRNA-1
chr6_3 CDS 45439663 45439869 45439663 ID=Msa0952140-mRNA-1:cds;Parent=Msa0952140-mRNA-1
Gene Sequence

>Msa0952140

ATGAGTATTTCAAACATTAGATGCATATGGAGATGGATGCTAGGCAGTTCAAGTACTCTGGCGTGCTTATTTTACGAGTGGAGTTGGGCACCTAGAGATTGCCTCTTGAGCTATGGTTTTGGAGCATACATTAATATTCTCCCGGGATCCGAATGGTCAGCTATAATGTTAACATATGGTTTCCCTCTTGCGATCATTGGCATGGCACTCAAGGGAAATGGAATCCCTAGAAGAAGTGCACCCATTCTACAAATGGTTCGTGAAGAGGTCACACAAGATGGAAAATATTGTTTGGTCTTGGTTTTCGAGGCTAAGGCTCTAAAGTTGTCAGATTTTGAACAGGGACAGATTTTGTATTCATCTTTCTTACCTCTTCATGAGCATAAGGAAACTCTTAATTATATCACTCTTGCAATGGAGGAATTCAGGAAGCGCATGGAAGCTTTAGAGAATCAATCAGGCAGTGGTTCTTGTAGTACTATTCGATCTCGTCACACTCAACATCCACATTATGATCCTGCTCTTGATGATCAATCAGAGGATGACCTCACCTAA
Protein sequence

>Msa0952140

MSISNIRCIWRWMLGSSSTLACLFYEWSWAPRDCLLSYGFGAYINILPGSEWSAIMLTYGFPLAIIGMALKGNGIPRRSAPILQMVREEVTQDGKYCLVLVFEAKALKLSDFEQGQILYSSFLPLHEHKETLNYITLAMEEFRKRMEALENQSGSGSCSTIRSRHTQHPHYDPALDDQSEDDLT*