AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0952540


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0952540 MtrunA17_Chr6g0469571 95.161 124 6 0 1 124 1 124 8.23e-75 218
Msa0952540 MtrunA17_Chr6g0469551 93.548 124 8 0 1 124 1 124 2.97e-74 216
Msa0952540 MtrunA17_Chr6g0469591 93.548 124 8 0 1 124 1 124 1.34e-73 214
Msa0952540 MtrunA17_Chr7g0233231 57.407 108 44 1 1 108 1 106 1.27e-38 125
Msa0952540 MtrunA17_Chr6g0469681 59.690 129 45 3 1 124 1 127 2.69e-38 125
Msa0952540 MtrunA17_Chr7g0233211 57.009 107 44 1 1 107 1 105 2.03e-37 122
Msa0952540 MtrunA17_Chr1g0202541 56.296 135 47 2 1 123 1 135 5.57e-37 122
Msa0952540 MtrunA17_Chr7g0233251 57.018 114 44 2 1 111 1 112 5.87e-37 121
Msa0952540 MtrunA17_Chr1g0202561 52.899 138 50 2 1 123 1 138 1.24e-36 122
Msa0952540 MtrunA17_Chr7g0233191 57.009 107 44 1 1 107 1 105 2.20e-36 120
Msa0952540 MtrunA17_Chr7g0233201 57.009 107 44 1 1 107 1 105 4.89e-36 119
Msa0952540 MtrunA17_Chr6g0469541 59.813 107 41 2 1 107 1 105 9.94e-36 118
Msa0952540 MtrunA17_Chr6g0469651 55.224 134 49 2 1 124 1 133 1.04e-35 119
Msa0952540 MtrunA17_Chr7g0233181 55.660 106 45 1 1 106 1 104 3.77e-35 117
Msa0952540 MtrunA17_Chr1g0202581 55.556 135 44 3 1 123 1 131 3.07e-34 115
Msa0952540 MtrunA17_Chr6g0469671 78.571 70 15 0 1 70 1 70 4.38e-34 114
Msa0952540 MtrunA17_Chr8g0387471 49.618 131 55 3 1 124 1 127 2.96e-33 112
Msa0952540 MtrunA17_Chr1g0202501 50.000 134 53 3 1 124 1 130 7.32e-33 112
Msa0952540 MtrunA17_Chr1g0204291 51.852 135 49 4 1 123 1 131 1.14e-32 111
Msa0952540 MtrunA17_Chr7g0248671 61.765 68 26 0 1 68 1 68 6.35e-24 90.5
Msa0952540 MtrunA17_Chr8g0348361 39.844 128 52 1 22 124 22 149 5.36e-15 67.0
Msa0952540 MtrunA17_Chr3g0120541 44.444 81 41 4 3 79 5 85 6.00e-14 63.2
Msa0952540 MtrunA17_Chr5g0404641 31.373 153 73 4 3 123 4 156 2.04e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0952540 AT1G01490.1 66.197 71 23 1 1 70 1 71 7.12e-25 93.6
Msa0952540 AT1G01490.2 66.197 71 23 1 1 70 1 71 7.12e-25 93.6
Msa0952540 AT1G01490.4 65.714 70 23 1 2 70 29 98 2.49e-24 92.8
Msa0952540 AT5G52740.1 55.714 70 29 1 1 70 1 68 7.66e-19 76.3
Msa0952540 AT5G52760.1 41.176 102 54 3 5 105 7 103 2.21e-15 67.8
Msa0952540 AT5G52750.1 45.455 66 36 0 2 67 4 69 5.14e-13 62.0
Msa0952540 AT1G63950.2 28.000 125 80 2 1 124 1 116 4.57e-11 56.2

Find 30 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACAGTCTCTGGCCTTTC+AGG 0.146342 6_3:-47129865 Msa0952540:intron
AACCAACAATTCTGATTTAT+TGG 0.192934 6_3:-47130063 Msa0952540:five_prime_UTR
AGACTTCAAGTAATTGAATT+TGG 0.223914 6_3:+47129315 None:intergenic
GACAAGAAATTAACCTTAAC+TGG 0.272704 6_3:-47129461 Msa0952540:CDS
TTGAAGATGAAAAGAGTTAG+AGG 0.293857 6_3:+47130126 None:intergenic
TGTATTTCTTATACTTTCAA+TGG 0.370006 6_3:+47129043 None:intergenic
ATTTGGTCCTTCTTCACATC+AGG 0.375669 6_3:+47129332 None:intergenic
GACCAATAAATCAGAATTGT+TGG 0.392701 6_3:+47130061 None:intergenic
ATATTCCTCTGTTTCAAGAA+TGG 0.394512 6_3:-47129252 Msa0952540:CDS
TAAAAGAAGAGCTAGGGTAT+TGG 0.399065 6_3:+47130092 None:intergenic
AGTTTGCTTACTACATGAAC+TGG 0.417883 6_3:+47129428 None:intergenic
TGGATCTGTGTCTCCAGTTA+AGG 0.425183 6_3:+47129448 None:intergenic
AGCTTTATTGAAATTAATGA+TGG 0.446473 6_3:-47130031 Msa0952540:intron
TAGTAAGCAAACTAAGGAAA+TGG 0.458647 6_3:-47129418 Msa0952540:CDS
ATGCTGAAATAGTTTCTGTT+GGG 0.476513 6_3:-47129391 Msa0952540:CDS
AAAGCTATGAAGACAGTCTC+TGG 0.487579 6_3:-47129874 Msa0952540:CDS
TACAATTTAAAAGAAGAGCT+AGG 0.497295 6_3:+47130085 None:intergenic
CATGCTGAAATAGTTTCTGT+TGG 0.500770 6_3:-47129392 Msa0952540:CDS
ATCTAAGCCTGATGTGAAGA+AGG 0.503469 6_3:-47129339 Msa0952540:CDS
TTCATGTAGTAAGCAAACTA+AGG 0.510687 6_3:-47129424 Msa0952540:CDS
CAGCATATGACACATCCAAC+AGG 0.518380 6_3:+47129221 None:intergenic
GAGGAATATTGAATATATTG+TGG 0.535752 6_3:+47129266 None:intergenic
ACAATTTAAAAGAAGAGCTA+GGG 0.536290 6_3:+47130086 None:intergenic
TTGTTCCATTCTTGAAACAG+AGG 0.577364 6_3:+47129247 None:intergenic
TGTTCAATTGTGCAAACAGA+GGG 0.585753 6_3:-47129088 Msa0952540:three_prime_UTR
ATTATTTGCAGGGGTAGAGT+CGG 0.592213 6_3:-47129501 Msa0952540:intron
ACATGTCGACATTACCTGAA+AGG 0.624790 6_3:+47129851 None:intergenic
AGAATGGAACAAGATCCTGT+TGG 0.628668 6_3:-47129236 Msa0952540:CDS
TGTTGCATATGATAATAATG+AGG 0.657518 6_3:+47129290 None:intergenic
GTGTTCAATTGTGCAAACAG+AGG 0.663444 6_3:-47129089 Msa0952540:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTATTTTCATATTATTTAT+TGG - chr6_3:47130010-47130029 Msa0952540:intron 10.0%
!!! TGTATTTTCATATTATTTAT+TGG - chr6_3:47130010-47130029 Msa0952540:intron 10.0%
!! AAAAAACTAATAGTAAAAGA+GGG + chr6_3:47129201-47129220 None:intergenic 15.0%
!!! TTATTTTGCTAAAATTGTAT+TGG - chr6_3:47129656-47129675 Msa0952540:intron 15.0%
!!! TTTTTATATTGTTTTTTGAG+TGG - chr6_3:47130048-47130067 Msa0952540:five_prime_UTR 15.0%
!! AAAAAACTAATAGTAAAAGA+GGG + chr6_3:47129201-47129220 None:intergenic 15.0%
!!! TTATTTTGCTAAAATTGTAT+TGG - chr6_3:47129656-47129675 Msa0952540:intron 15.0%
!!! TTTTTATATTGTTTTTTGAG+TGG - chr6_3:47130048-47130067 Msa0952540:five_prime_UTR 15.0%
!!! AGCTTTATTGAAATTAATGA+TGG - chr6_3:47129152-47129171 Msa0952540:three_prime_UTR 20.0%
!! GAAAAAACTAATAGTAAAAG+AGG + chr6_3:47129202-47129221 None:intergenic 20.0%
!! TTCACATTATTTAATCTTGA+GGG - chr6_3:47129461-47129480 Msa0952540:CDS 20.0%
!! TCAAATTATGAGGTTAAAAA+GGG + chr6_3:47129503-47129522 None:intergenic 20.0%
!! TTCAAATTATGAGGTTAAAA+AGG + chr6_3:47129504-47129523 None:intergenic 20.0%
!! TAAATGATGTTCAAATTATG+AGG + chr6_3:47129513-47129532 None:intergenic 20.0%
!! TGTATTTCTTATACTTTCAA+TGG + chr6_3:47130143-47130162 None:intergenic 20.0%
!!! AGCTTTATTGAAATTAATGA+TGG - chr6_3:47129152-47129171 Msa0952540:three_prime_UTR 20.0%
!! GAAAAAACTAATAGTAAAAG+AGG + chr6_3:47129202-47129221 None:intergenic 20.0%
!! TTCACATTATTTAATCTTGA+GGG - chr6_3:47129461-47129480 Msa0952540:CDS 20.0%
!! TCAAATTATGAGGTTAAAAA+GGG + chr6_3:47129503-47129522 None:intergenic 20.0%
!! TTCAAATTATGAGGTTAAAA+AGG + chr6_3:47129504-47129523 None:intergenic 20.0%
!! TAAATGATGTTCAAATTATG+AGG + chr6_3:47129513-47129532 None:intergenic 20.0%
!! TGTATTTCTTATACTTTCAA+TGG + chr6_3:47130143-47130162 None:intergenic 20.0%
! ACAATTTAAAAGAAGAGCTA+GGG + chr6_3:47129100-47129119 None:intergenic 25.0%
! TACAATTTAAAAGAAGAGCT+AGG + chr6_3:47129101-47129120 None:intergenic 25.0%
! AACCAACAATTCTGATTTAT+TGG - chr6_3:47129120-47129139 Msa0952540:three_prime_UTR 25.0%
!!! ACATGTCTAGTTTCAATTTT+TGG - chr6_3:47129349-47129368 Msa0952540:CDS 25.0%
! CTCAAGATTAAATAATGTGA+AGG + chr6_3:47129462-47129481 None:intergenic 25.0%
! CTTCACATTATTTAATCTTG+AGG - chr6_3:47129460-47129479 Msa0952540:CDS 25.0%
!! TGTATTGGTATATTATTTGC+AGG - chr6_3:47129671-47129690 Msa0952540:intron 25.0%
!! GTATTGGTATATTATTTGCA+GGG - chr6_3:47129672-47129691 Msa0952540:intron 25.0%
! TATTGGTATATTATTTGCAG+GGG - chr6_3:47129673-47129692 Msa0952540:intron 25.0%
! AGACTTCAAGTAATTGAATT+TGG + chr6_3:47129871-47129890 None:intergenic 25.0%
! TGTTGCATATGATAATAATG+AGG + chr6_3:47129896-47129915 None:intergenic 25.0%
! GAGGAATATTGAATATATTG+TGG + chr6_3:47129920-47129939 None:intergenic 25.0%
! ACAATTTAAAAGAAGAGCTA+GGG + chr6_3:47129100-47129119 None:intergenic 25.0%
! TACAATTTAAAAGAAGAGCT+AGG + chr6_3:47129101-47129120 None:intergenic 25.0%
! AACCAACAATTCTGATTTAT+TGG - chr6_3:47129120-47129139 Msa0952540:three_prime_UTR 25.0%
!!! ACATGTCTAGTTTCAATTTT+TGG - chr6_3:47129349-47129368 Msa0952540:CDS 25.0%
! CTCAAGATTAAATAATGTGA+AGG + chr6_3:47129462-47129481 None:intergenic 25.0%
! CTTCACATTATTTAATCTTG+AGG - chr6_3:47129460-47129479 Msa0952540:CDS 25.0%
!! TGTATTGGTATATTATTTGC+AGG - chr6_3:47129671-47129690 Msa0952540:intron 25.0%
!! GTATTGGTATATTATTTGCA+GGG - chr6_3:47129672-47129691 Msa0952540:intron 25.0%
! TATTGGTATATTATTTGCAG+GGG - chr6_3:47129673-47129692 Msa0952540:intron 25.0%
! AGACTTCAAGTAATTGAATT+TGG + chr6_3:47129871-47129890 None:intergenic 25.0%
! TGTTGCATATGATAATAATG+AGG + chr6_3:47129896-47129915 None:intergenic 25.0%
! GAGGAATATTGAATATATTG+TGG + chr6_3:47129920-47129939 None:intergenic 25.0%
TTGAAGATGAAAAGAGTTAG+AGG + chr6_3:47129060-47129079 None:intergenic 30.0%
GACCAATAAATCAGAATTGT+TGG + chr6_3:47129125-47129144 None:intergenic 30.0%
!!! GTTACAGAAACTAGTTTTGA+AGG - chr6_3:47129257-47129276 Msa0952540:CDS 30.0%
GACAAGAAATTAACCTTAAC+TGG - chr6_3:47129722-47129741 Msa0952540:intron 30.0%
TTCATGTAGTAAGCAAACTA+AGG - chr6_3:47129759-47129778 Msa0952540:intron 30.0%
TAGTAAGCAAACTAAGGAAA+TGG - chr6_3:47129765-47129784 Msa0952540:intron 30.0%
ATGCTGAAATAGTTTCTGTT+GGG - chr6_3:47129792-47129811 Msa0952540:intron 30.0%
! TTCTTCTTTTCATCTTTTGC+AGG + chr6_3:47129818-47129837 None:intergenic 30.0%
ATATTCCTCTGTTTCAAGAA+TGG - chr6_3:47129931-47129950 Msa0952540:intron 30.0%
TTGAAGATGAAAAGAGTTAG+AGG + chr6_3:47129060-47129079 None:intergenic 30.0%
GACCAATAAATCAGAATTGT+TGG + chr6_3:47129125-47129144 None:intergenic 30.0%
!!! GTTACAGAAACTAGTTTTGA+AGG - chr6_3:47129257-47129276 Msa0952540:CDS 30.0%
GACAAGAAATTAACCTTAAC+TGG - chr6_3:47129722-47129741 Msa0952540:intron 30.0%
TTCATGTAGTAAGCAAACTA+AGG - chr6_3:47129759-47129778 Msa0952540:intron 30.0%
TAGTAAGCAAACTAAGGAAA+TGG - chr6_3:47129765-47129784 Msa0952540:intron 30.0%
ATGCTGAAATAGTTTCTGTT+GGG - chr6_3:47129792-47129811 Msa0952540:intron 30.0%
! TTCTTCTTTTCATCTTTTGC+AGG + chr6_3:47129818-47129837 None:intergenic 30.0%
ATATTCCTCTGTTTCAAGAA+TGG - chr6_3:47129931-47129950 Msa0952540:intron 30.0%
TAAAAGAAGAGCTAGGGTAT+TGG + chr6_3:47129094-47129113 None:intergenic 35.0%
AGTAAAAGAGGGAATTCACA+AGG + chr6_3:47129190-47129209 None:intergenic 35.0%
!!! ACAGAAACTAGTTTTGAAGG+TGG - chr6_3:47129260-47129279 Msa0952540:CDS 35.0%
! TGTCTTCATAGCTTTTTGCT+TGG + chr6_3:47129304-47129323 None:intergenic 35.0%
! AGTTTGCTTACTACATGAAC+TGG + chr6_3:47129758-47129777 None:intergenic 35.0%
CATGCTGAAATAGTTTCTGT+TGG - chr6_3:47129791-47129810 Msa0952540:intron 35.0%
TTGTTCCATTCTTGAAACAG+AGG + chr6_3:47129939-47129958 None:intergenic 35.0%
TGTTCAATTGTGCAAACAGA+GGG - chr6_3:47130095-47130114 Msa0952540:five_prime_UTR 35.0%
TAAAAGAAGAGCTAGGGTAT+TGG + chr6_3:47129094-47129113 None:intergenic 35.0%
AGTAAAAGAGGGAATTCACA+AGG + chr6_3:47129190-47129209 None:intergenic 35.0%
!!! ACAGAAACTAGTTTTGAAGG+TGG - chr6_3:47129260-47129279 Msa0952540:CDS 35.0%
! TGTCTTCATAGCTTTTTGCT+TGG + chr6_3:47129304-47129323 None:intergenic 35.0%
! AGTTTGCTTACTACATGAAC+TGG + chr6_3:47129758-47129777 None:intergenic 35.0%
CATGCTGAAATAGTTTCTGT+TGG - chr6_3:47129791-47129810 Msa0952540:intron 35.0%
TTGTTCCATTCTTGAAACAG+AGG + chr6_3:47129939-47129958 None:intergenic 35.0%
TGTTCAATTGTGCAAACAGA+GGG - chr6_3:47130095-47130114 Msa0952540:five_prime_UTR 35.0%
AAAGCTATGAAGACAGTCTC+TGG - chr6_3:47129309-47129328 Msa0952540:CDS 40.0%
ACATGTCGACATTACCTGAA+AGG + chr6_3:47129335-47129354 None:intergenic 40.0%
ATTATTTGCAGGGGTAGAGT+CGG - chr6_3:47129682-47129701 Msa0952540:intron 40.0%
ATCTAAGCCTGATGTGAAGA+AGG - chr6_3:47129844-47129863 Msa0952540:intron 40.0%
ATTTGGTCCTTCTTCACATC+AGG + chr6_3:47129854-47129873 None:intergenic 40.0%
AGAATGGAACAAGATCCTGT+TGG - chr6_3:47129947-47129966 Msa0952540:intron 40.0%
GTGTTCAATTGTGCAAACAG+AGG - chr6_3:47130094-47130113 Msa0952540:five_prime_UTR 40.0%
AAAGCTATGAAGACAGTCTC+TGG - chr6_3:47129309-47129328 Msa0952540:CDS 40.0%
ACATGTCGACATTACCTGAA+AGG + chr6_3:47129335-47129354 None:intergenic 40.0%
ATTATTTGCAGGGGTAGAGT+CGG - chr6_3:47129682-47129701 Msa0952540:intron 40.0%
ATCTAAGCCTGATGTGAAGA+AGG - chr6_3:47129844-47129863 Msa0952540:intron 40.0%
ATTTGGTCCTTCTTCACATC+AGG + chr6_3:47129854-47129873 None:intergenic 40.0%
AGAATGGAACAAGATCCTGT+TGG - chr6_3:47129947-47129966 Msa0952540:intron 40.0%
GTGTTCAATTGTGCAAACAG+AGG - chr6_3:47130094-47130113 Msa0952540:five_prime_UTR 40.0%
GAGGGAATTCACAAGGATCT+AGG + chr6_3:47129183-47129202 None:intergenic 45.0%
TGGATCTGTGTCTCCAGTTA+AGG + chr6_3:47129738-47129757 None:intergenic 45.0%
CAGCATATGACACATCCAAC+AGG + chr6_3:47129965-47129984 None:intergenic 45.0%
GAGGGAATTCACAAGGATCT+AGG + chr6_3:47129183-47129202 None:intergenic 45.0%
TGGATCTGTGTCTCCAGTTA+AGG + chr6_3:47129738-47129757 None:intergenic 45.0%
CAGCATATGACACATCCAAC+AGG + chr6_3:47129965-47129984 None:intergenic 45.0%
!!! TTTATTTTTAAATTGAAAAA+TGG + chr6_3:47129562-47129581 None:intergenic 5.0%
!!! TTTATTTTTAAATTGAAAAA+TGG + chr6_3:47129562-47129581 None:intergenic 5.0%
! AAGACAGTCTCTGGCCTTTC+AGG - chr6_3:47129318-47129337 Msa0952540:CDS 50.0%
! AAGACAGTCTCTGGCCTTTC+AGG - chr6_3:47129318-47129337 Msa0952540:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 47129034 47130171 47129034 ID=Msa0952540;Name=Msa0952540
chr6_3 mRNA 47129034 47130171 47129034 ID=Msa0952540-mRNA-1;Parent=Msa0952540;Name=Msa0952540-mRNA-1;_AED=0.34;_eAED=0.34;_QI=134|1|1|1|1|1|3|186|124
chr6_3 exon 47129034 47129512 47129034 ID=Msa0952540-mRNA-1:exon:10401;Parent=Msa0952540-mRNA-1
chr6_3 exon 47129866 47129941 47129866 ID=Msa0952540-mRNA-1:exon:10400;Parent=Msa0952540-mRNA-1
chr6_3 exon 47130032 47130171 47130032 ID=Msa0952540-mRNA-1:exon:10399;Parent=Msa0952540-mRNA-1
chr6_3 five_prime_UTR 47130038 47130171 47130038 ID=Msa0952540-mRNA-1:five_prime_utr;Parent=Msa0952540-mRNA-1
chr6_3 CDS 47130032 47130037 47130032 ID=Msa0952540-mRNA-1:cds;Parent=Msa0952540-mRNA-1
chr6_3 CDS 47129866 47129941 47129866 ID=Msa0952540-mRNA-1:cds;Parent=Msa0952540-mRNA-1
chr6_3 CDS 47129220 47129512 47129220 ID=Msa0952540-mRNA-1:cds;Parent=Msa0952540-mRNA-1
chr6_3 three_prime_UTR 47129034 47129219 47129034 ID=Msa0952540-mRNA-1:three_prime_utr;Parent=Msa0952540-mRNA-1
Gene Sequence

>Msa0952540

ATGATGAAACTAGTTTTGAAGGTGGATTTACATGATGATAGAACCAAGCAAAAAGCTATGAAGACAGTCTCTGGCCTTTCAGGGGTAGAGTCGGTTTCTATAGACATGAAAGACAAGAAATTAACCTTAACTGGAGACACAGATCCAGTTCATGTAGTAAGCAAACTAAGGAAATGGTGTCATGCTGAAATAGTTTCTGTTGGGCCTGCAAAAGATGAAAAGAAGAAAGAAGAATCTAAGCCTGATGTGAAGAAGGACCAAATTCAATTACTTGAAGTCTATCCTCATTATTATCATATGCAACAACCACAATATATTCAATATTCCTCTGTTTCAAGAATGGAACAAGATCCTGTTGGATGTGTCATATGCTGA

Protein sequence

>Msa0952540

MMKLVLKVDLHDDRTKQKAMKTVSGLSGVESVSIDMKDKKLTLTGDTDPVHVVSKLRKWCHAEIVSVGPAKDEKKKEESKPDVKKDQIQLLEVYPHYYHMQQPQYIQYSSVSRMEQDPVGCVIC*