AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0952560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0952560 MtrunA17_Chr6g0469571 95.161 124 6 0 1 124 1 124 8.23e-75 218
Msa0952560 MtrunA17_Chr6g0469551 93.548 124 8 0 1 124 1 124 2.97e-74 216
Msa0952560 MtrunA17_Chr6g0469591 93.548 124 8 0 1 124 1 124 1.34e-73 214
Msa0952560 MtrunA17_Chr7g0233231 57.407 108 44 1 1 108 1 106 1.27e-38 125
Msa0952560 MtrunA17_Chr6g0469681 59.690 129 45 3 1 124 1 127 2.69e-38 125
Msa0952560 MtrunA17_Chr7g0233211 57.009 107 44 1 1 107 1 105 2.03e-37 122
Msa0952560 MtrunA17_Chr1g0202541 56.296 135 47 2 1 123 1 135 5.57e-37 122
Msa0952560 MtrunA17_Chr7g0233251 57.018 114 44 2 1 111 1 112 5.87e-37 121
Msa0952560 MtrunA17_Chr1g0202561 52.899 138 50 2 1 123 1 138 1.24e-36 122
Msa0952560 MtrunA17_Chr7g0233191 57.009 107 44 1 1 107 1 105 2.20e-36 120
Msa0952560 MtrunA17_Chr7g0233201 57.009 107 44 1 1 107 1 105 4.89e-36 119
Msa0952560 MtrunA17_Chr6g0469541 59.813 107 41 2 1 107 1 105 9.94e-36 118
Msa0952560 MtrunA17_Chr6g0469651 55.224 134 49 2 1 124 1 133 1.04e-35 119
Msa0952560 MtrunA17_Chr7g0233181 55.660 106 45 1 1 106 1 104 3.77e-35 117
Msa0952560 MtrunA17_Chr1g0202581 55.556 135 44 3 1 123 1 131 3.07e-34 115
Msa0952560 MtrunA17_Chr6g0469671 78.571 70 15 0 1 70 1 70 4.38e-34 114
Msa0952560 MtrunA17_Chr8g0387471 49.618 131 55 3 1 124 1 127 2.96e-33 112
Msa0952560 MtrunA17_Chr1g0202501 50.000 134 53 3 1 124 1 130 7.32e-33 112
Msa0952560 MtrunA17_Chr1g0204291 51.852 135 49 4 1 123 1 131 1.14e-32 111
Msa0952560 MtrunA17_Chr7g0248671 61.765 68 26 0 1 68 1 68 6.35e-24 90.5
Msa0952560 MtrunA17_Chr8g0348361 39.844 128 52 1 22 124 22 149 5.36e-15 67.0
Msa0952560 MtrunA17_Chr3g0120541 44.444 81 41 4 3 79 5 85 6.00e-14 63.2
Msa0952560 MtrunA17_Chr5g0404641 31.373 153 73 4 3 123 4 156 2.04e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0952560 AT1G01490.1 66.197 71 23 1 1 70 1 71 7.12e-25 93.6
Msa0952560 AT1G01490.2 66.197 71 23 1 1 70 1 71 7.12e-25 93.6
Msa0952560 AT1G01490.4 65.714 70 23 1 2 70 29 98 2.49e-24 92.8
Msa0952560 AT5G52740.1 55.714 70 29 1 1 70 1 68 7.66e-19 76.3
Msa0952560 AT5G52760.1 41.176 102 54 3 5 105 7 103 2.21e-15 67.8
Msa0952560 AT5G52750.1 45.455 66 36 0 2 67 4 69 5.14e-13 62.0
Msa0952560 AT1G63950.2 28.000 125 80 2 1 124 1 116 4.57e-11 56.2

Find 30 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACAGTCTCTGGCCTTTC+AGG 0.146342 6_3:-47140488 Msa0952560:intron
AACCAACAATTCTGATTTAT+TGG 0.192934 6_3:-47140686 Msa0952560:five_prime_UTR
AGACTTCAAGTAATTGAATT+TGG 0.223914 6_3:+47139938 None:intergenic
GACAAGAAATTAACCTTAAC+TGG 0.272704 6_3:-47140084 Msa0952560:CDS
TTGAAGATGAAAAGAGTTAG+AGG 0.293857 6_3:+47140749 None:intergenic
TGTATTTCTTATACTTTCAA+TGG 0.370006 6_3:+47139666 None:intergenic
ATTTGGTCCTTCTTCACATC+AGG 0.375669 6_3:+47139955 None:intergenic
GACCAATAAATCAGAATTGT+TGG 0.392701 6_3:+47140684 None:intergenic
ATATTCCTCTGTTTCAAGAA+TGG 0.394512 6_3:-47139875 Msa0952560:CDS
TAAAAGAAGAGCTAGGGTAT+TGG 0.399065 6_3:+47140715 None:intergenic
AGTTTGCTTACTACATGAAC+TGG 0.417883 6_3:+47140051 None:intergenic
TGGATCTGTGTCTCCAGTTA+AGG 0.425183 6_3:+47140071 None:intergenic
AGCTTTATTGAAATTAATGA+TGG 0.446473 6_3:-47140654 Msa0952560:intron
TAGTAAGCAAACTAAGGAAA+TGG 0.458647 6_3:-47140041 Msa0952560:CDS
ATGCTGAAATAGTTTCTGTT+GGG 0.476513 6_3:-47140014 Msa0952560:CDS
AAAGCTATGAAGACAGTCTC+TGG 0.487579 6_3:-47140497 Msa0952560:CDS
TACAATTTAAAAGAAGAGCT+AGG 0.497295 6_3:+47140708 None:intergenic
CATGCTGAAATAGTTTCTGT+TGG 0.500770 6_3:-47140015 Msa0952560:CDS
ATCTAAGCCTGATGTGAAGA+AGG 0.503469 6_3:-47139962 Msa0952560:CDS
TTCATGTAGTAAGCAAACTA+AGG 0.510687 6_3:-47140047 Msa0952560:CDS
CAGCATATGACACATCCAAC+AGG 0.518380 6_3:+47139844 None:intergenic
GAGGAATATTGAATATATTG+TGG 0.535752 6_3:+47139889 None:intergenic
ACAATTTAAAAGAAGAGCTA+GGG 0.536290 6_3:+47140709 None:intergenic
TTGTTCCATTCTTGAAACAG+AGG 0.577364 6_3:+47139870 None:intergenic
TGTTCAATTGTGCAAACAGA+GGG 0.585753 6_3:-47139711 Msa0952560:three_prime_UTR
ATTATTTGCAGGGGTAGAGT+CGG 0.592213 6_3:-47140124 Msa0952560:intron
ACATGTCGACATTACCTGAA+AGG 0.624790 6_3:+47140474 None:intergenic
AGAATGGAACAAGATCCTGT+TGG 0.628668 6_3:-47139859 Msa0952560:CDS
TGTTGCATATGATAATAATG+AGG 0.657518 6_3:+47139913 None:intergenic
GTGTTCAATTGTGCAAACAG+AGG 0.663444 6_3:-47139712 Msa0952560:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTATTTTCATATTATTTAT+TGG - chr6_3:47140633-47140652 Msa0952560:intron 10.0%
!!! TGTATTTTCATATTATTTAT+TGG - chr6_3:47140633-47140652 Msa0952560:intron 10.0%
!! AAAAAACTAATAGTAAAAGA+GGG + chr6_3:47139824-47139843 None:intergenic 15.0%
!!! TTATTTTGCTAAAATTGTAT+TGG - chr6_3:47140279-47140298 Msa0952560:intron 15.0%
!!! TTTTTATATTGTTTTTTGAG+TGG - chr6_3:47140671-47140690 Msa0952560:five_prime_UTR 15.0%
!! AAAAAACTAATAGTAAAAGA+GGG + chr6_3:47139824-47139843 None:intergenic 15.0%
!!! TTATTTTGCTAAAATTGTAT+TGG - chr6_3:47140279-47140298 Msa0952560:intron 15.0%
!!! TTTTTATATTGTTTTTTGAG+TGG - chr6_3:47140671-47140690 Msa0952560:five_prime_UTR 15.0%
!!! AGCTTTATTGAAATTAATGA+TGG - chr6_3:47139775-47139794 Msa0952560:three_prime_UTR 20.0%
!! GAAAAAACTAATAGTAAAAG+AGG + chr6_3:47139825-47139844 None:intergenic 20.0%
!! TTCACATTATTTAATCTTGA+GGG - chr6_3:47140084-47140103 Msa0952560:CDS 20.0%
!! TCAAATTATGAGGTTAAAAA+GGG + chr6_3:47140126-47140145 None:intergenic 20.0%
!! TTCAAATTATGAGGTTAAAA+AGG + chr6_3:47140127-47140146 None:intergenic 20.0%
!! TAAATGATGTTCAAATTATG+AGG + chr6_3:47140136-47140155 None:intergenic 20.0%
!! TGTATTTCTTATACTTTCAA+TGG + chr6_3:47140766-47140785 None:intergenic 20.0%
!!! AGCTTTATTGAAATTAATGA+TGG - chr6_3:47139775-47139794 Msa0952560:three_prime_UTR 20.0%
!! GAAAAAACTAATAGTAAAAG+AGG + chr6_3:47139825-47139844 None:intergenic 20.0%
!! TTCACATTATTTAATCTTGA+GGG - chr6_3:47140084-47140103 Msa0952560:CDS 20.0%
!! TCAAATTATGAGGTTAAAAA+GGG + chr6_3:47140126-47140145 None:intergenic 20.0%
!! TTCAAATTATGAGGTTAAAA+AGG + chr6_3:47140127-47140146 None:intergenic 20.0%
!! TAAATGATGTTCAAATTATG+AGG + chr6_3:47140136-47140155 None:intergenic 20.0%
!! TGTATTTCTTATACTTTCAA+TGG + chr6_3:47140766-47140785 None:intergenic 20.0%
! ACAATTTAAAAGAAGAGCTA+GGG + chr6_3:47139723-47139742 None:intergenic 25.0%
! TACAATTTAAAAGAAGAGCT+AGG + chr6_3:47139724-47139743 None:intergenic 25.0%
! AACCAACAATTCTGATTTAT+TGG - chr6_3:47139743-47139762 Msa0952560:three_prime_UTR 25.0%
!!! ACATGTCTAGTTTCAATTTT+TGG - chr6_3:47139972-47139991 Msa0952560:CDS 25.0%
! CTCAAGATTAAATAATGTGA+AGG + chr6_3:47140085-47140104 None:intergenic 25.0%
! CTTCACATTATTTAATCTTG+AGG - chr6_3:47140083-47140102 Msa0952560:CDS 25.0%
!! TGTATTGGTATATTATTTGC+AGG - chr6_3:47140294-47140313 Msa0952560:intron 25.0%
!! GTATTGGTATATTATTTGCA+GGG - chr6_3:47140295-47140314 Msa0952560:intron 25.0%
! TATTGGTATATTATTTGCAG+GGG - chr6_3:47140296-47140315 Msa0952560:intron 25.0%
! AGACTTCAAGTAATTGAATT+TGG + chr6_3:47140494-47140513 None:intergenic 25.0%
! TGTTGCATATGATAATAATG+AGG + chr6_3:47140519-47140538 None:intergenic 25.0%
! GAGGAATATTGAATATATTG+TGG + chr6_3:47140543-47140562 None:intergenic 25.0%
! ACAATTTAAAAGAAGAGCTA+GGG + chr6_3:47139723-47139742 None:intergenic 25.0%
! TACAATTTAAAAGAAGAGCT+AGG + chr6_3:47139724-47139743 None:intergenic 25.0%
! AACCAACAATTCTGATTTAT+TGG - chr6_3:47139743-47139762 Msa0952560:three_prime_UTR 25.0%
!!! ACATGTCTAGTTTCAATTTT+TGG - chr6_3:47139972-47139991 Msa0952560:CDS 25.0%
! CTCAAGATTAAATAATGTGA+AGG + chr6_3:47140085-47140104 None:intergenic 25.0%
! CTTCACATTATTTAATCTTG+AGG - chr6_3:47140083-47140102 Msa0952560:CDS 25.0%
!! TGTATTGGTATATTATTTGC+AGG - chr6_3:47140294-47140313 Msa0952560:intron 25.0%
!! GTATTGGTATATTATTTGCA+GGG - chr6_3:47140295-47140314 Msa0952560:intron 25.0%
! TATTGGTATATTATTTGCAG+GGG - chr6_3:47140296-47140315 Msa0952560:intron 25.0%
! AGACTTCAAGTAATTGAATT+TGG + chr6_3:47140494-47140513 None:intergenic 25.0%
! TGTTGCATATGATAATAATG+AGG + chr6_3:47140519-47140538 None:intergenic 25.0%
! GAGGAATATTGAATATATTG+TGG + chr6_3:47140543-47140562 None:intergenic 25.0%
TTGAAGATGAAAAGAGTTAG+AGG + chr6_3:47139683-47139702 None:intergenic 30.0%
GACCAATAAATCAGAATTGT+TGG + chr6_3:47139748-47139767 None:intergenic 30.0%
!!! GTTACAGAAACTAGTTTTGA+AGG - chr6_3:47139880-47139899 Msa0952560:CDS 30.0%
GACAAGAAATTAACCTTAAC+TGG - chr6_3:47140345-47140364 Msa0952560:intron 30.0%
TTCATGTAGTAAGCAAACTA+AGG - chr6_3:47140382-47140401 Msa0952560:intron 30.0%
TAGTAAGCAAACTAAGGAAA+TGG - chr6_3:47140388-47140407 Msa0952560:intron 30.0%
ATGCTGAAATAGTTTCTGTT+GGG - chr6_3:47140415-47140434 Msa0952560:intron 30.0%
! TTCTTCTTTTCATCTTTTGC+AGG + chr6_3:47140441-47140460 None:intergenic 30.0%
ATATTCCTCTGTTTCAAGAA+TGG - chr6_3:47140554-47140573 Msa0952560:intron 30.0%
TTGAAGATGAAAAGAGTTAG+AGG + chr6_3:47139683-47139702 None:intergenic 30.0%
GACCAATAAATCAGAATTGT+TGG + chr6_3:47139748-47139767 None:intergenic 30.0%
!!! GTTACAGAAACTAGTTTTGA+AGG - chr6_3:47139880-47139899 Msa0952560:CDS 30.0%
GACAAGAAATTAACCTTAAC+TGG - chr6_3:47140345-47140364 Msa0952560:intron 30.0%
TTCATGTAGTAAGCAAACTA+AGG - chr6_3:47140382-47140401 Msa0952560:intron 30.0%
TAGTAAGCAAACTAAGGAAA+TGG - chr6_3:47140388-47140407 Msa0952560:intron 30.0%
ATGCTGAAATAGTTTCTGTT+GGG - chr6_3:47140415-47140434 Msa0952560:intron 30.0%
! TTCTTCTTTTCATCTTTTGC+AGG + chr6_3:47140441-47140460 None:intergenic 30.0%
ATATTCCTCTGTTTCAAGAA+TGG - chr6_3:47140554-47140573 Msa0952560:intron 30.0%
TAAAAGAAGAGCTAGGGTAT+TGG + chr6_3:47139717-47139736 None:intergenic 35.0%
AGTAAAAGAGGGAATTCACA+AGG + chr6_3:47139813-47139832 None:intergenic 35.0%
!!! ACAGAAACTAGTTTTGAAGG+TGG - chr6_3:47139883-47139902 Msa0952560:CDS 35.0%
! TGTCTTCATAGCTTTTTGCT+TGG + chr6_3:47139927-47139946 None:intergenic 35.0%
! AGTTTGCTTACTACATGAAC+TGG + chr6_3:47140381-47140400 None:intergenic 35.0%
CATGCTGAAATAGTTTCTGT+TGG - chr6_3:47140414-47140433 Msa0952560:intron 35.0%
TTGTTCCATTCTTGAAACAG+AGG + chr6_3:47140562-47140581 None:intergenic 35.0%
TGTTCAATTGTGCAAACAGA+GGG - chr6_3:47140718-47140737 Msa0952560:five_prime_UTR 35.0%
TAAAAGAAGAGCTAGGGTAT+TGG + chr6_3:47139717-47139736 None:intergenic 35.0%
AGTAAAAGAGGGAATTCACA+AGG + chr6_3:47139813-47139832 None:intergenic 35.0%
!!! ACAGAAACTAGTTTTGAAGG+TGG - chr6_3:47139883-47139902 Msa0952560:CDS 35.0%
! TGTCTTCATAGCTTTTTGCT+TGG + chr6_3:47139927-47139946 None:intergenic 35.0%
! AGTTTGCTTACTACATGAAC+TGG + chr6_3:47140381-47140400 None:intergenic 35.0%
CATGCTGAAATAGTTTCTGT+TGG - chr6_3:47140414-47140433 Msa0952560:intron 35.0%
TTGTTCCATTCTTGAAACAG+AGG + chr6_3:47140562-47140581 None:intergenic 35.0%
TGTTCAATTGTGCAAACAGA+GGG - chr6_3:47140718-47140737 Msa0952560:five_prime_UTR 35.0%
AAAGCTATGAAGACAGTCTC+TGG - chr6_3:47139932-47139951 Msa0952560:CDS 40.0%
ACATGTCGACATTACCTGAA+AGG + chr6_3:47139958-47139977 None:intergenic 40.0%
ATTATTTGCAGGGGTAGAGT+CGG - chr6_3:47140305-47140324 Msa0952560:intron 40.0%
ATCTAAGCCTGATGTGAAGA+AGG - chr6_3:47140467-47140486 Msa0952560:intron 40.0%
ATTTGGTCCTTCTTCACATC+AGG + chr6_3:47140477-47140496 None:intergenic 40.0%
AGAATGGAACAAGATCCTGT+TGG - chr6_3:47140570-47140589 Msa0952560:intron 40.0%
GTGTTCAATTGTGCAAACAG+AGG - chr6_3:47140717-47140736 Msa0952560:five_prime_UTR 40.0%
AAAGCTATGAAGACAGTCTC+TGG - chr6_3:47139932-47139951 Msa0952560:CDS 40.0%
ACATGTCGACATTACCTGAA+AGG + chr6_3:47139958-47139977 None:intergenic 40.0%
ATTATTTGCAGGGGTAGAGT+CGG - chr6_3:47140305-47140324 Msa0952560:intron 40.0%
ATCTAAGCCTGATGTGAAGA+AGG - chr6_3:47140467-47140486 Msa0952560:intron 40.0%
ATTTGGTCCTTCTTCACATC+AGG + chr6_3:47140477-47140496 None:intergenic 40.0%
AGAATGGAACAAGATCCTGT+TGG - chr6_3:47140570-47140589 Msa0952560:intron 40.0%
GTGTTCAATTGTGCAAACAG+AGG - chr6_3:47140717-47140736 Msa0952560:five_prime_UTR 40.0%
GAGGGAATTCACAAGGATCT+AGG + chr6_3:47139806-47139825 None:intergenic 45.0%
TGGATCTGTGTCTCCAGTTA+AGG + chr6_3:47140361-47140380 None:intergenic 45.0%
CAGCATATGACACATCCAAC+AGG + chr6_3:47140588-47140607 None:intergenic 45.0%
GAGGGAATTCACAAGGATCT+AGG + chr6_3:47139806-47139825 None:intergenic 45.0%
TGGATCTGTGTCTCCAGTTA+AGG + chr6_3:47140361-47140380 None:intergenic 45.0%
CAGCATATGACACATCCAAC+AGG + chr6_3:47140588-47140607 None:intergenic 45.0%
!!! TTTATTTTTAAATTGAAAAA+TGG + chr6_3:47140185-47140204 None:intergenic 5.0%
!!! TTTATTTTTAAATTGAAAAA+TGG + chr6_3:47140185-47140204 None:intergenic 5.0%
! AAGACAGTCTCTGGCCTTTC+AGG - chr6_3:47139941-47139960 Msa0952560:CDS 50.0%
! AAGACAGTCTCTGGCCTTTC+AGG - chr6_3:47139941-47139960 Msa0952560:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 47139657 47140794 47139657 ID=Msa0952560;Name=Msa0952560
chr6_3 mRNA 47139657 47140794 47139657 ID=Msa0952560-mRNA-1;Parent=Msa0952560;Name=Msa0952560-mRNA-1;_AED=0.34;_eAED=0.34;_QI=134|1|1|1|1|1|3|186|124
chr6_3 exon 47139657 47140135 47139657 ID=Msa0952560-mRNA-1:exon:10404;Parent=Msa0952560-mRNA-1
chr6_3 exon 47140489 47140564 47140489 ID=Msa0952560-mRNA-1:exon:10403;Parent=Msa0952560-mRNA-1
chr6_3 exon 47140655 47140794 47140655 ID=Msa0952560-mRNA-1:exon:10402;Parent=Msa0952560-mRNA-1
chr6_3 five_prime_UTR 47140661 47140794 47140661 ID=Msa0952560-mRNA-1:five_prime_utr;Parent=Msa0952560-mRNA-1
chr6_3 CDS 47140655 47140660 47140655 ID=Msa0952560-mRNA-1:cds;Parent=Msa0952560-mRNA-1
chr6_3 CDS 47140489 47140564 47140489 ID=Msa0952560-mRNA-1:cds;Parent=Msa0952560-mRNA-1
chr6_3 CDS 47139843 47140135 47139843 ID=Msa0952560-mRNA-1:cds;Parent=Msa0952560-mRNA-1
chr6_3 three_prime_UTR 47139657 47139842 47139657 ID=Msa0952560-mRNA-1:three_prime_utr;Parent=Msa0952560-mRNA-1
Gene Sequence

>Msa0952560

ATGATGAAACTAGTTTTGAAGGTGGATTTACATGATGATAGAACCAAGCAAAAAGCTATGAAGACAGTCTCTGGCCTTTCAGGGGTAGAGTCGGTTTCTATAGACATGAAAGACAAGAAATTAACCTTAACTGGAGACACAGATCCAGTTCATGTAGTAAGCAAACTAAGGAAATGGTGTCATGCTGAAATAGTTTCTGTTGGGCCTGCAAAAGATGAAAAGAAGAAAGAAGAATCTAAGCCTGATGTGAAGAAGGACCAAATTCAATTACTTGAAGTCTATCCTCATTATTATCATATGCAACAACCACAATATATTCAATATTCCTCTGTTTCAAGAATGGAACAAGATCCTGTTGGATGTGTCATATGCTGA

Protein sequence

>Msa0952560

MMKLVLKVDLHDDRTKQKAMKTVSGLSGVESVSIDMKDKKLTLTGDTDPVHVVSKLRKWCHAEIVSVGPAKDEKKKEESKPDVKKDQIQLLEVYPHYYHMQQPQYIQYSSVSRMEQDPVGCVIC*