AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0954490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0954490 MtrunA17_Chr6g0486641 98.842 259 3 0 1 259 229 487 0.0 537
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0954490 AT4G28910.3 44.922 256 71 8 1 241 214 414 1.01e-53 179
Msa0954490 AT4G28910.1 44.922 256 71 8 1 241 214 414 1.01e-53 179
Msa0954490 AT4G28910.2 44.922 256 71 8 1 241 214 414 1.01e-53 179

Find 99 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTCCTTTCACGTCTTC+AGG 0.217298 6_3:-55153729 None:intergenic
TTCAGATAGCAATTCGGTTT+TGG 0.226700 6_3:+55154034 Msa0954490:CDS
CAGTTTCATCCTTCCTTTGC+AGG 0.256187 6_3:+55153229 Msa0954490:CDS
CCTTCACCTTTCGACTGTTC+TGG 0.269268 6_3:-55153777 None:intergenic
TTCACTTGTTGAGCAGAAAA+AGG 0.274328 6_3:-55152992 None:intergenic
GCAAGTAATTAAAGTGAAAT+AGG 0.279259 6_3:-55154225 None:intergenic
GTGGATACCCAAGGCAGATT+TGG 0.307968 6_3:-55153873 None:intergenic
AACCGAATTGCTATCTGAAT+TGG 0.313360 6_3:-55154030 None:intergenic
AAACATGCAAACATGACTTA+TGG 0.318828 6_3:+55152965 Msa0954490:exon
ATTGTAGTTGTATTTAATAA+TGG 0.334890 6_3:+55154264 Msa0954490:three_prime_UTR
ACTCCAGGTATTTGAGGGCT+TGG 0.360299 6_3:-55153067 None:intergenic
TCAAATGGAAGGACAATATC+AGG 0.363492 6_3:+55153903 Msa0954490:CDS
CAATATCAGGTGTTACTTAC+AGG 0.371934 6_3:+55153916 Msa0954490:CDS
ACGCACAGACGATTCTGATT+TGG 0.379964 6_3:-55153950 None:intergenic
ACGCTGTTTCCCATCACCTT+TGG 0.386142 6_3:-55153679 None:intergenic
GGAAGCTTGCCGATGATGTT+TGG 0.388261 6_3:+55153148 Msa0954490:CDS
CAAAAGAGTCAAACTCTGTT+GGG 0.393194 6_3:+55153041 Msa0954490:CDS
TAGTTCTCAACAAGAGAATC+TGG 0.398865 6_3:+55154141 Msa0954490:three_prime_UTR
CATCCTTCCTTTGCAGGGAG+AGG 0.404447 6_3:+55153235 Msa0954490:CDS
GCCTTCATACGGCATGGCCT+CGG 0.418342 6_3:-55153640 None:intergenic
TGGATACCCAAGGCAGATTT+GGG 0.424790 6_3:-55153872 None:intergenic
AATGGGCAGAAGAGATTTAC+CGG 0.429122 6_3:+55152923 Msa0954490:five_prime_UTR
TTCCTACCCAAATCTGCCTT+GGG 0.439337 6_3:+55153866 Msa0954490:CDS
TCACAATTACCTGAGTTAGC+TGG 0.441376 6_3:-55153259 None:intergenic
AGCTTGCCGAATGGTAATCT+TGG 0.447111 6_3:+55154059 Msa0954490:CDS
TGCATCACTCCAGGTATTTG+AGG 0.452843 6_3:-55153073 None:intergenic
CATATGATGCCAACTACAAC+CGG 0.452935 6_3:+55153094 Msa0954490:CDS
TTGTGAAGAGGAGCCTTCTT+CGG 0.455235 6_3:-55153706 None:intergenic
GTCGCTGACTCAAGCGCCAA+TGG 0.463056 6_3:+55152905 Msa0954490:five_prime_UTR
AACCAATTCAGATAGCAATT+CGG 0.466685 6_3:+55154028 Msa0954490:CDS
TTCACATCCGCGGTAAGCCC+TGG 0.469941 6_3:-55153828 None:intergenic
GTATCCACCACAAGCTCAAA+TGG 0.471490 6_3:+55153888 Msa0954490:CDS
GCACGTTCACCGGTTGTAGT+TGG 0.472212 6_3:-55153103 None:intergenic
CACAATTACCTGAGTTAGCT+GGG 0.474611 6_3:-55153258 None:intergenic
TTCTTCTGATGCTTCGAAAG+AGG 0.478218 6_3:+55152874 Msa0954490:five_prime_UTR
TTGAGCTCCTGCACGTTCAC+CGG 0.483475 6_3:-55153113 None:intergenic
CTTACCGCGGATGTGAAATT+CGG 0.486162 6_3:+55153834 Msa0954490:CDS
AAGGAAGCAGCACAAACCTC+AGG 0.489023 6_3:+55153742 Msa0954490:CDS
GTTCCTACCCAAATCTGCCT+TGG 0.490143 6_3:+55153865 Msa0954490:CDS
GCAAAAGAGTCAAACTCTGT+TGG 0.492235 6_3:+55153040 Msa0954490:CDS
GCATCACTCCAGGTATTTGA+GGG 0.494358 6_3:-55153072 None:intergenic
GGTAAATCTCTTCTGCCCAT+TGG 0.495423 6_3:-55152921 None:intergenic
AAACAGCGTGTCACCGAAGA+AGG 0.502399 6_3:+55153693 Msa0954490:CDS
GTATGTTCAGCTTCCTATGT+TGG 0.509167 6_3:+55153177 Msa0954490:CDS
TGTCCTTCCATTTGAGCTTG+TGG 0.512711 6_3:-55153895 None:intergenic
GATGTGAAATTCGGAGGAAG+TGG 0.522473 6_3:+55153843 Msa0954490:CDS
TCCGAGGCCATGCCGTATGA+AGG 0.523566 6_3:+55153639 Msa0954490:CDS
GCACCAAGCCCTCAAATACC+TGG 0.525712 6_3:+55153064 Msa0954490:CDS
ATTACTATCATCTACCCTCC+AGG 0.526129 6_3:+55154101 Msa0954490:three_prime_UTR
AGGCCATGCCGTATGAAGGC+AGG 0.527342 6_3:+55153643 Msa0954490:CDS
TCGCTGACTCAAGCGCCAAT+GGG 0.528288 6_3:+55152906 Msa0954490:five_prime_UTR
GGGCCTCTCCCTGCAAAGGA+AGG 0.535109 6_3:-55153238 None:intergenic
CGGCCTGCCTTCATACGGCA+TGG 0.542332 6_3:-55153646 None:intergenic
CACGTCTTCAGGTTGTGAAG+AGG 0.545832 6_3:-55153718 None:intergenic
AGTTTCATCCTTCCTTTGCA+GGG 0.548370 6_3:+55153230 Msa0954490:CDS
CTGGTGCATCTTTGCCCCTG+AGG 0.550568 6_3:-55153758 None:intergenic
CTAGAACGAACCAAAGGTGA+TGG 0.556734 6_3:+55153669 Msa0954490:CDS
CCTTCCATTTGAGCTTGTGG+TGG 0.558546 6_3:-55153892 None:intergenic
GCATGACGAACAAAATCCTC+AGG 0.559639 6_3:-55153993 None:intergenic
GATGCTTCGAAAGAGGTTCG+TGG 0.563001 6_3:+55152881 Msa0954490:five_prime_UTR
GGAGCTCAATCTGTGAGTAA+TGG 0.566490 6_3:+55153127 Msa0954490:CDS
TCACAACCTGAAGACGTGAA+AGG 0.567779 6_3:+55153723 Msa0954490:CDS
AACATAGGAAGCTGAACATA+CGG 0.571663 6_3:-55153175 None:intergenic
AGCTGGGCCTCTCCCTGCAA+AGG 0.572843 6_3:-55153242 None:intergenic
GGCATCATATGCATCACTCC+AGG 0.573713 6_3:-55153082 None:intergenic
TCACCTTTGGTTCGTTCTAG+CGG 0.576196 6_3:-55153666 None:intergenic
AGGAAGCAGCACAAACCTCA+GGG 0.577639 6_3:+55153743 Msa0954490:CDS
GAGCTATCCTTGTCCAACAT+AGG 0.579094 6_3:-55153190 None:intergenic
GATGAACCAAGATTACCATT+CGG 0.580457 6_3:-55154065 None:intergenic
TGTTGGACAAGGATAGCTCA+TGG 0.582770 6_3:+55153194 Msa0954490:CDS
GAGCTTGTGGTGGATACCCA+AGG 0.584613 6_3:-55153882 None:intergenic
AGAATCGTCTGTGCGTGTCA+TGG 0.586566 6_3:+55153957 Msa0954490:CDS
ACTACAACCGGTGAACGTGC+AGG 0.587532 6_3:+55153106 Msa0954490:CDS
TAGAACGAACCAAAGGTGAT+GGG 0.588598 6_3:+55153670 Msa0954490:CDS
CCAGAACAGTCGAAAGGTGA+AGG 0.589987 6_3:+55153777 Msa0954490:CDS
AAGGAAGGATGAAACTGTTG+AGG 0.591748 6_3:-55153223 None:intergenic
GGGAGAGGCCCAGCTAACTC+AGG 0.593652 6_3:+55153250 Msa0954490:CDS
AGGAAGGATGAAACTGTTGA+GGG 0.596334 6_3:-55153222 None:intergenic
GTTGGACAAGGATAGCTCAT+GGG 0.597490 6_3:+55153195 Msa0954490:CDS
AAAAGAGTCAAACTCTGTTG+GGG 0.602879 6_3:+55153042 Msa0954490:CDS
TACGGATGTCCAAACATCAT+CGG 0.603516 6_3:-55153157 None:intergenic
TCTAGCGGCCTGCCTTCATA+CGG 0.615919 6_3:-55153651 None:intergenic
TCAGCTTCCTATGTTGGACA+AGG 0.624986 6_3:+55153183 Msa0954490:CDS
CCACCACAAGCTCAAATGGA+AGG 0.636715 6_3:+55153892 Msa0954490:CDS
ACCGCGGATGTGAAATTCGG+AGG 0.643018 6_3:+55153837 Msa0954490:CDS
TCCTCCGAATTTCACATCCG+CGG 0.647146 6_3:-55153838 None:intergenic
TTGGACAAGGATAGCTCATG+GGG 0.649802 6_3:+55153196 Msa0954490:CDS
TATATAGAGTACAACCTGGA+GGG 0.659464 6_3:-55154115 None:intergenic
TGGTTCTCACATGACTCCTG+AGG 0.661496 6_3:+55153977 Msa0954490:CDS
TACCCAAGGCAGATTTGGGT+AGG 0.663102 6_3:-55153868 None:intergenic
ACATCTAAACAATATATCCG+AGG 0.671648 6_3:+55153623 Msa0954490:CDS
GATGCACCAGAACAGTCGAA+AGG 0.674903 6_3:+55153771 Msa0954490:CDS
GATGAGCGGACCTTACACTT+CGG 0.678313 6_3:+55153018 Msa0954490:CDS
AGGCCGCTAGAACGAACCAA+AGG 0.687826 6_3:+55153663 Msa0954490:CDS
ACAATATATAGAGTACAACC+TGG 0.689288 6_3:-55154119 None:intergenic
CATTAAGCCAGGGCTTACCG+CGG 0.690110 6_3:+55153821 Msa0954490:CDS
GGAAGCAGCACAAACCTCAG+GGG 0.704276 6_3:+55153744 Msa0954490:CDS
CAACAAGTGAATATGATGAG+CGG 0.706230 6_3:+55153004 Msa0954490:CDS
ATATATAGAGTACAACCTGG+AGG 0.721270 6_3:-55154116 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTGTTAGTTAAAATGTTT+TGG + chr6_3:55153355-55153374 Msa0954490:intron 15.0%
!! CAAATAGAAAGAAAAAAAAA+TGG - chr6_3:55154205-55154224 None:intergenic 15.0%
!! ATTGTAGTTGTATTTAATAA+TGG + chr6_3:55154264-55154283 Msa0954490:three_prime_UTR 15.0%
!!! AATTGTTAGTTAAAATGTTT+TGG + chr6_3:55153355-55153374 Msa0954490:intron 15.0%
!! CAAATAGAAAGAAAAAAAAA+TGG - chr6_3:55154205-55154224 None:intergenic 15.0%
!! ATTGTAGTTGTATTTAATAA+TGG + chr6_3:55154264-55154283 Msa0954490:three_prime_UTR 15.0%
!! AACAATTTATGAATTTGACT+TGG - chr6_3:55153342-55153361 None:intergenic 20.0%
!!! TGAACCAAATATTCTAAAAT+TGG + chr6_3:55153425-55153444 Msa0954490:intron 20.0%
!! TGTAATTTCCAAAAAAAACT+CGG - chr6_3:55153571-55153590 None:intergenic 20.0%
!! CTTAATGTTTGAAAAATCAA+TGG - chr6_3:55153808-55153827 None:intergenic 20.0%
!! AACAATTTATGAATTTGACT+TGG - chr6_3:55153342-55153361 None:intergenic 20.0%
!!! TGAACCAAATATTCTAAAAT+TGG + chr6_3:55153425-55153444 Msa0954490:intron 20.0%
!! TGTAATTTCCAAAAAAAACT+CGG - chr6_3:55153571-55153590 None:intergenic 20.0%
!! CTTAATGTTTGAAAAATCAA+TGG - chr6_3:55153808-55153827 None:intergenic 20.0%
!!! ATGTTTGAAATCTTTTTCTG+TGG - chr6_3:55152951-55152970 None:intergenic 25.0%
!!! CCAACCAATTTTAGAATATT+TGG - chr6_3:55153432-55153451 None:intergenic 25.0%
!!! CCAAATATTCTAAAATTGGT+TGG + chr6_3:55153429-55153448 Msa0954490:intron 25.0%
! AATGTTTGAAAAATCAATGG+TGG - chr6_3:55153805-55153824 None:intergenic 25.0%
!!! ATTTTTCAAACATTAAGCCA+GGG + chr6_3:55153811-55153830 Msa0954490:CDS 25.0%
!! TGGAATTTCCATAATTTTTG+TGG + chr6_3:55154161-55154180 Msa0954490:three_prime_UTR 25.0%
! ATTCATCTCCACAAAAATTA+TGG - chr6_3:55154172-55154191 None:intergenic 25.0%
! GCAAGTAATTAAAGTGAAAT+AGG - chr6_3:55154228-55154247 None:intergenic 25.0%
!!! ATGTTTGAAATCTTTTTCTG+TGG - chr6_3:55152951-55152970 None:intergenic 25.0%
!!! CCAACCAATTTTAGAATATT+TGG - chr6_3:55153432-55153451 None:intergenic 25.0%
!!! CCAAATATTCTAAAATTGGT+TGG + chr6_3:55153429-55153448 Msa0954490:intron 25.0%
! AATGTTTGAAAAATCAATGG+TGG - chr6_3:55153805-55153824 None:intergenic 25.0%
!!! ATTTTTCAAACATTAAGCCA+GGG + chr6_3:55153811-55153830 Msa0954490:CDS 25.0%
!! TGGAATTTCCATAATTTTTG+TGG + chr6_3:55154161-55154180 Msa0954490:three_prime_UTR 25.0%
! ATTCATCTCCACAAAAATTA+TGG - chr6_3:55154172-55154191 None:intergenic 25.0%
! GCAAGTAATTAAAGTGAAAT+AGG - chr6_3:55154228-55154247 None:intergenic 25.0%
AAACATGCAAACATGACTTA+TGG + chr6_3:55152965-55152984 Msa0954490:exon 30.0%
TCAGGTAATTGTGATTTGAA+TGG + chr6_3:55153268-55153287 Msa0954490:intron 30.0%
!!! GATACTTACCGAGTTTTTTT+TGG + chr6_3:55153560-55153579 Msa0954490:intron 30.0%
ACATCTAAACAATATATCCG+AGG + chr6_3:55153623-55153642 Msa0954490:CDS 30.0%
!! GATTTTTCAAACATTAAGCC+AGG + chr6_3:55153810-55153829 Msa0954490:CDS 30.0%
AACCAATTCAGATAGCAATT+CGG + chr6_3:55154028-55154047 Msa0954490:CDS 30.0%
ACAATATATAGAGTACAACC+TGG - chr6_3:55154122-55154141 None:intergenic 30.0%
AAACATGCAAACATGACTTA+TGG + chr6_3:55152965-55152984 Msa0954490:exon 30.0%
TCAGGTAATTGTGATTTGAA+TGG + chr6_3:55153268-55153287 Msa0954490:intron 30.0%
!!! GATACTTACCGAGTTTTTTT+TGG + chr6_3:55153560-55153579 Msa0954490:intron 30.0%
ACATCTAAACAATATATCCG+AGG + chr6_3:55153623-55153642 Msa0954490:CDS 30.0%
!! GATTTTTCAAACATTAAGCC+AGG + chr6_3:55153810-55153829 Msa0954490:CDS 30.0%
AACCAATTCAGATAGCAATT+CGG + chr6_3:55154028-55154047 Msa0954490:CDS 30.0%
ACAATATATAGAGTACAACC+TGG - chr6_3:55154122-55154141 None:intergenic 30.0%
! GAAATCTTTTTCTGTGGATC+CGG - chr6_3:55152945-55152964 None:intergenic 35.0%
! TTCACTTGTTGAGCAGAAAA+AGG - chr6_3:55152995-55153014 None:intergenic 35.0%
CAACAAGTGAATATGATGAG+CGG + chr6_3:55153004-55153023 Msa0954490:CDS 35.0%
CAAAAGAGTCAAACTCTGTT+GGG + chr6_3:55153041-55153060 Msa0954490:CDS 35.0%
AAAAGAGTCAAACTCTGTTG+GGG + chr6_3:55153042-55153061 Msa0954490:CDS 35.0%
AACATAGGAAGCTGAACATA+CGG - chr6_3:55153178-55153197 None:intergenic 35.0%
ATATGTTCCTGACTGCATTA+TGG + chr6_3:55153385-55153404 Msa0954490:intron 35.0%
GGTTCAGTTTACAAAACTTG+CGG - chr6_3:55153411-55153430 None:intergenic 35.0%
! TCTAAAATTGGTTGGAAGAC+TGG + chr6_3:55153437-55153456 Msa0954490:intron 35.0%
GAAGACTGGCATATTGAATT+GGG + chr6_3:55153451-55153470 Msa0954490:intron 35.0%
TCAAATGGAAGGACAATATC+AGG + chr6_3:55153903-55153922 Msa0954490:CDS 35.0%
!! CAATATCAGGTGTTACTTAC+AGG + chr6_3:55153916-55153935 Msa0954490:CDS 35.0%
AACCGAATTGCTATCTGAAT+TGG - chr6_3:55154033-55154052 None:intergenic 35.0%
! TTCAGATAGCAATTCGGTTT+TGG + chr6_3:55154034-55154053 Msa0954490:CDS 35.0%
! TCAGATAGCAATTCGGTTTT+GGG + chr6_3:55154035-55154054 Msa0954490:CDS 35.0%
GATGAACCAAGATTACCATT+CGG - chr6_3:55154068-55154087 None:intergenic 35.0%
TATATAGAGTACAACCTGGA+GGG - chr6_3:55154118-55154137 None:intergenic 35.0%
ATATATAGAGTACAACCTGG+AGG - chr6_3:55154119-55154138 None:intergenic 35.0%
TAGTTCTCAACAAGAGAATC+TGG + chr6_3:55154141-55154160 Msa0954490:three_prime_UTR 35.0%
! GAAATCTTTTTCTGTGGATC+CGG - chr6_3:55152945-55152964 None:intergenic 35.0%
! TTCACTTGTTGAGCAGAAAA+AGG - chr6_3:55152995-55153014 None:intergenic 35.0%
CAACAAGTGAATATGATGAG+CGG + chr6_3:55153004-55153023 Msa0954490:CDS 35.0%
CAAAAGAGTCAAACTCTGTT+GGG + chr6_3:55153041-55153060 Msa0954490:CDS 35.0%
AAAAGAGTCAAACTCTGTTG+GGG + chr6_3:55153042-55153061 Msa0954490:CDS 35.0%
AACATAGGAAGCTGAACATA+CGG - chr6_3:55153178-55153197 None:intergenic 35.0%
ATATGTTCCTGACTGCATTA+TGG + chr6_3:55153385-55153404 Msa0954490:intron 35.0%
GGTTCAGTTTACAAAACTTG+CGG - chr6_3:55153411-55153430 None:intergenic 35.0%
! TCTAAAATTGGTTGGAAGAC+TGG + chr6_3:55153437-55153456 Msa0954490:intron 35.0%
GAAGACTGGCATATTGAATT+GGG + chr6_3:55153451-55153470 Msa0954490:intron 35.0%
TCAAATGGAAGGACAATATC+AGG + chr6_3:55153903-55153922 Msa0954490:CDS 35.0%
!! CAATATCAGGTGTTACTTAC+AGG + chr6_3:55153916-55153935 Msa0954490:CDS 35.0%
AACCGAATTGCTATCTGAAT+TGG - chr6_3:55154033-55154052 None:intergenic 35.0%
! TTCAGATAGCAATTCGGTTT+TGG + chr6_3:55154034-55154053 Msa0954490:CDS 35.0%
! TCAGATAGCAATTCGGTTTT+GGG + chr6_3:55154035-55154054 Msa0954490:CDS 35.0%
GATGAACCAAGATTACCATT+CGG - chr6_3:55154068-55154087 None:intergenic 35.0%
TATATAGAGTACAACCTGGA+GGG - chr6_3:55154118-55154137 None:intergenic 35.0%
ATATATAGAGTACAACCTGG+AGG - chr6_3:55154119-55154138 None:intergenic 35.0%
TAGTTCTCAACAAGAGAATC+TGG + chr6_3:55154141-55154160 Msa0954490:three_prime_UTR 35.0%
TTCTTCTGATGCTTCGAAAG+AGG + chr6_3:55152874-55152893 Msa0954490:five_prime_UTR 40.0%
AATGGGCAGAAGAGATTTAC+CGG + chr6_3:55152923-55152942 Msa0954490:five_prime_UTR 40.0%
GCAAAAGAGTCAAACTCTGT+TGG + chr6_3:55153040-55153059 Msa0954490:CDS 40.0%
CATATGATGCCAACTACAAC+CGG + chr6_3:55153094-55153113 Msa0954490:CDS 40.0%
TACGGATGTCCAAACATCAT+CGG - chr6_3:55153160-55153179 None:intergenic 40.0%
GTATGTTCAGCTTCCTATGT+TGG + chr6_3:55153177-55153196 Msa0954490:CDS 40.0%
AGGAAGGATGAAACTGTTGA+GGG - chr6_3:55153225-55153244 None:intergenic 40.0%
AAGGAAGGATGAAACTGTTG+AGG - chr6_3:55153226-55153245 None:intergenic 40.0%
AGTTTCATCCTTCCTTTGCA+GGG + chr6_3:55153230-55153249 Msa0954490:CDS 40.0%
CACAATTACCTGAGTTAGCT+GGG - chr6_3:55153261-55153280 None:intergenic 40.0%
TCACAATTACCTGAGTTAGC+TGG - chr6_3:55153262-55153281 None:intergenic 40.0%
!! AAGTTCTGAGTCGGGAATAT+TGG - chr6_3:55153312-55153331 None:intergenic 40.0%
!! GATCTTTGAAGTTCTGAGTC+GGG - chr6_3:55153320-55153339 None:intergenic 40.0%
!! GGATCTTTGAAGTTCTGAGT+CGG - chr6_3:55153321-55153340 None:intergenic 40.0%
GGAAGACTGGCATATTGAAT+TGG + chr6_3:55153450-55153469 Msa0954490:intron 40.0%
ACTGGCATATTGAATTGGGT+TGG + chr6_3:55153455-55153474 Msa0954490:intron 40.0%
TAGAACGAACCAAAGGTGAT+GGG + chr6_3:55153670-55153689 Msa0954490:CDS 40.0%
ATTACTATCATCTACCCTCC+AGG + chr6_3:55154101-55154120 Msa0954490:three_prime_UTR 40.0%
TTCTTCTGATGCTTCGAAAG+AGG + chr6_3:55152874-55152893 Msa0954490:five_prime_UTR 40.0%
AATGGGCAGAAGAGATTTAC+CGG + chr6_3:55152923-55152942 Msa0954490:five_prime_UTR 40.0%
GCAAAAGAGTCAAACTCTGT+TGG + chr6_3:55153040-55153059 Msa0954490:CDS 40.0%
CATATGATGCCAACTACAAC+CGG + chr6_3:55153094-55153113 Msa0954490:CDS 40.0%
TACGGATGTCCAAACATCAT+CGG - chr6_3:55153160-55153179 None:intergenic 40.0%
GTATGTTCAGCTTCCTATGT+TGG + chr6_3:55153177-55153196 Msa0954490:CDS 40.0%
AGGAAGGATGAAACTGTTGA+GGG - chr6_3:55153225-55153244 None:intergenic 40.0%
AAGGAAGGATGAAACTGTTG+AGG - chr6_3:55153226-55153245 None:intergenic 40.0%
AGTTTCATCCTTCCTTTGCA+GGG + chr6_3:55153230-55153249 Msa0954490:CDS 40.0%
CACAATTACCTGAGTTAGCT+GGG - chr6_3:55153261-55153280 None:intergenic 40.0%
TCACAATTACCTGAGTTAGC+TGG - chr6_3:55153262-55153281 None:intergenic 40.0%
!! AAGTTCTGAGTCGGGAATAT+TGG - chr6_3:55153312-55153331 None:intergenic 40.0%
!! GATCTTTGAAGTTCTGAGTC+GGG - chr6_3:55153320-55153339 None:intergenic 40.0%
!! GGATCTTTGAAGTTCTGAGT+CGG - chr6_3:55153321-55153340 None:intergenic 40.0%
GGAAGACTGGCATATTGAAT+TGG + chr6_3:55153450-55153469 Msa0954490:intron 40.0%
ACTGGCATATTGAATTGGGT+TGG + chr6_3:55153455-55153474 Msa0954490:intron 40.0%
TAGAACGAACCAAAGGTGAT+GGG + chr6_3:55153670-55153689 Msa0954490:CDS 40.0%
ATTACTATCATCTACCCTCC+AGG + chr6_3:55154101-55154120 Msa0954490:three_prime_UTR 40.0%
GGTAAATCTCTTCTGCCCAT+TGG - chr6_3:55152924-55152943 None:intergenic 45.0%
! GACTCTTTTGCCGAAGTGTA+AGG - chr6_3:55153031-55153050 None:intergenic 45.0%
GCATCACTCCAGGTATTTGA+GGG - chr6_3:55153075-55153094 None:intergenic 45.0%
TGCATCACTCCAGGTATTTG+AGG - chr6_3:55153076-55153095 None:intergenic 45.0%
GGAGCTCAATCTGTGAGTAA+TGG + chr6_3:55153127-55153146 Msa0954490:CDS 45.0%
TCAGCTTCCTATGTTGGACA+AGG + chr6_3:55153183-55153202 Msa0954490:CDS 45.0%
GAGCTATCCTTGTCCAACAT+AGG - chr6_3:55153193-55153212 None:intergenic 45.0%
TGTTGGACAAGGATAGCTCA+TGG + chr6_3:55153194-55153213 Msa0954490:CDS 45.0%
GTTGGACAAGGATAGCTCAT+GGG + chr6_3:55153195-55153214 Msa0954490:CDS 45.0%
TTGGACAAGGATAGCTCATG+GGG + chr6_3:55153196-55153215 Msa0954490:CDS 45.0%
CAGTTTCATCCTTCCTTTGC+AGG + chr6_3:55153229-55153248 Msa0954490:CDS 45.0%
! TCACCTTTGGTTCGTTCTAG+CGG - chr6_3:55153669-55153688 None:intergenic 45.0%
CTAGAACGAACCAAAGGTGA+TGG + chr6_3:55153669-55153688 Msa0954490:CDS 45.0%
TTGTGAAGAGGAGCCTTCTT+CGG - chr6_3:55153709-55153728 None:intergenic 45.0%
TCACAACCTGAAGACGTGAA+AGG + chr6_3:55153723-55153742 Msa0954490:CDS 45.0%
CTTACCGCGGATGTGAAATT+CGG + chr6_3:55153834-55153853 Msa0954490:CDS 45.0%
GATGTGAAATTCGGAGGAAG+TGG + chr6_3:55153843-55153862 Msa0954490:CDS 45.0%
TTCCTACCCAAATCTGCCTT+GGG + chr6_3:55153866-55153885 Msa0954490:CDS 45.0%
TGGATACCCAAGGCAGATTT+GGG - chr6_3:55153875-55153894 None:intergenic 45.0%
GTATCCACCACAAGCTCAAA+TGG + chr6_3:55153888-55153907 Msa0954490:CDS 45.0%
TGTCCTTCCATTTGAGCTTG+TGG - chr6_3:55153898-55153917 None:intergenic 45.0%
ACGCACAGACGATTCTGATT+TGG - chr6_3:55153953-55153972 None:intergenic 45.0%
GCATGACGAACAAAATCCTC+AGG - chr6_3:55153996-55154015 None:intergenic 45.0%
AGCTTGCCGAATGGTAATCT+TGG + chr6_3:55154059-55154078 Msa0954490:CDS 45.0%
GGTAAATCTCTTCTGCCCAT+TGG - chr6_3:55152924-55152943 None:intergenic 45.0%
! GACTCTTTTGCCGAAGTGTA+AGG - chr6_3:55153031-55153050 None:intergenic 45.0%
GCATCACTCCAGGTATTTGA+GGG - chr6_3:55153075-55153094 None:intergenic 45.0%
TGCATCACTCCAGGTATTTG+AGG - chr6_3:55153076-55153095 None:intergenic 45.0%
GGAGCTCAATCTGTGAGTAA+TGG + chr6_3:55153127-55153146 Msa0954490:CDS 45.0%
TCAGCTTCCTATGTTGGACA+AGG + chr6_3:55153183-55153202 Msa0954490:CDS 45.0%
GAGCTATCCTTGTCCAACAT+AGG - chr6_3:55153193-55153212 None:intergenic 45.0%
TGTTGGACAAGGATAGCTCA+TGG + chr6_3:55153194-55153213 Msa0954490:CDS 45.0%
GTTGGACAAGGATAGCTCAT+GGG + chr6_3:55153195-55153214 Msa0954490:CDS 45.0%
TTGGACAAGGATAGCTCATG+GGG + chr6_3:55153196-55153215 Msa0954490:CDS 45.0%
CAGTTTCATCCTTCCTTTGC+AGG + chr6_3:55153229-55153248 Msa0954490:CDS 45.0%
! TCACCTTTGGTTCGTTCTAG+CGG - chr6_3:55153669-55153688 None:intergenic 45.0%
CTAGAACGAACCAAAGGTGA+TGG + chr6_3:55153669-55153688 Msa0954490:CDS 45.0%
TTGTGAAGAGGAGCCTTCTT+CGG - chr6_3:55153709-55153728 None:intergenic 45.0%
TCACAACCTGAAGACGTGAA+AGG + chr6_3:55153723-55153742 Msa0954490:CDS 45.0%
CTTACCGCGGATGTGAAATT+CGG + chr6_3:55153834-55153853 Msa0954490:CDS 45.0%
GATGTGAAATTCGGAGGAAG+TGG + chr6_3:55153843-55153862 Msa0954490:CDS 45.0%
TTCCTACCCAAATCTGCCTT+GGG + chr6_3:55153866-55153885 Msa0954490:CDS 45.0%
TGGATACCCAAGGCAGATTT+GGG - chr6_3:55153875-55153894 None:intergenic 45.0%
GTATCCACCACAAGCTCAAA+TGG + chr6_3:55153888-55153907 Msa0954490:CDS 45.0%
TGTCCTTCCATTTGAGCTTG+TGG - chr6_3:55153898-55153917 None:intergenic 45.0%
ACGCACAGACGATTCTGATT+TGG - chr6_3:55153953-55153972 None:intergenic 45.0%
GCATGACGAACAAAATCCTC+AGG - chr6_3:55153996-55154015 None:intergenic 45.0%
AGCTTGCCGAATGGTAATCT+TGG + chr6_3:55154059-55154078 Msa0954490:CDS 45.0%
GATGCTTCGAAAGAGGTTCG+TGG + chr6_3:55152881-55152900 Msa0954490:five_prime_UTR 50.0%
GATGAGCGGACCTTACACTT+CGG + chr6_3:55153018-55153037 Msa0954490:CDS 50.0%
ACTCCAGGTATTTGAGGGCT+TGG - chr6_3:55153070-55153089 None:intergenic 50.0%
GGCATCATATGCATCACTCC+AGG - chr6_3:55153085-55153104 None:intergenic 50.0%
GGAAGCTTGCCGATGATGTT+TGG + chr6_3:55153148-55153167 Msa0954490:CDS 50.0%
! ACGCTGTTTCCCATCACCTT+TGG - chr6_3:55153682-55153701 None:intergenic 50.0%
AAACAGCGTGTCACCGAAGA+AGG + chr6_3:55153693-55153712 Msa0954490:CDS 50.0%
!! CACGTCTTCAGGTTGTGAAG+AGG - chr6_3:55153721-55153740 None:intergenic 50.0%
CTGCTTCCTTTCACGTCTTC+AGG - chr6_3:55153732-55153751 None:intergenic 50.0%
AAGGAAGCAGCACAAACCTC+AGG + chr6_3:55153742-55153761 Msa0954490:CDS 50.0%
AGGAAGCAGCACAAACCTCA+GGG + chr6_3:55153743-55153762 Msa0954490:CDS 50.0%
GATGCACCAGAACAGTCGAA+AGG + chr6_3:55153771-55153790 Msa0954490:CDS 50.0%
CCTTCACCTTTCGACTGTTC+TGG - chr6_3:55153780-55153799 None:intergenic 50.0%
CCAGAACAGTCGAAAGGTGA+AGG + chr6_3:55153777-55153796 Msa0954490:CDS 50.0%
TCCTCCGAATTTCACATCCG+CGG - chr6_3:55153841-55153860 None:intergenic 50.0%
GTTCCTACCCAAATCTGCCT+TGG + chr6_3:55153865-55153884 Msa0954490:CDS 50.0%
TACCCAAGGCAGATTTGGGT+AGG - chr6_3:55153871-55153890 None:intergenic 50.0%
GTGGATACCCAAGGCAGATT+TGG - chr6_3:55153876-55153895 None:intergenic 50.0%
CCTTCCATTTGAGCTTGTGG+TGG - chr6_3:55153895-55153914 None:intergenic 50.0%
CCACCACAAGCTCAAATGGA+AGG + chr6_3:55153892-55153911 Msa0954490:CDS 50.0%
AGAATCGTCTGTGCGTGTCA+TGG + chr6_3:55153957-55153976 Msa0954490:CDS 50.0%
TGGTTCTCACATGACTCCTG+AGG + chr6_3:55153977-55153996 Msa0954490:CDS 50.0%
!!! GTTTTGGGAAGCTTGCCGAA+TGG + chr6_3:55154050-55154069 Msa0954490:CDS 50.0%
GATGCTTCGAAAGAGGTTCG+TGG + chr6_3:55152881-55152900 Msa0954490:five_prime_UTR 50.0%
GATGAGCGGACCTTACACTT+CGG + chr6_3:55153018-55153037 Msa0954490:CDS 50.0%
ACTCCAGGTATTTGAGGGCT+TGG - chr6_3:55153070-55153089 None:intergenic 50.0%
GGCATCATATGCATCACTCC+AGG - chr6_3:55153085-55153104 None:intergenic 50.0%
GGAAGCTTGCCGATGATGTT+TGG + chr6_3:55153148-55153167 Msa0954490:CDS 50.0%
! ACGCTGTTTCCCATCACCTT+TGG - chr6_3:55153682-55153701 None:intergenic 50.0%
AAACAGCGTGTCACCGAAGA+AGG + chr6_3:55153693-55153712 Msa0954490:CDS 50.0%
!! CACGTCTTCAGGTTGTGAAG+AGG - chr6_3:55153721-55153740 None:intergenic 50.0%
CTGCTTCCTTTCACGTCTTC+AGG - chr6_3:55153732-55153751 None:intergenic 50.0%
AAGGAAGCAGCACAAACCTC+AGG + chr6_3:55153742-55153761 Msa0954490:CDS 50.0%
AGGAAGCAGCACAAACCTCA+GGG + chr6_3:55153743-55153762 Msa0954490:CDS 50.0%
GATGCACCAGAACAGTCGAA+AGG + chr6_3:55153771-55153790 Msa0954490:CDS 50.0%
CCTTCACCTTTCGACTGTTC+TGG - chr6_3:55153780-55153799 None:intergenic 50.0%
CCAGAACAGTCGAAAGGTGA+AGG + chr6_3:55153777-55153796 Msa0954490:CDS 50.0%
TCCTCCGAATTTCACATCCG+CGG - chr6_3:55153841-55153860 None:intergenic 50.0%
GTTCCTACCCAAATCTGCCT+TGG + chr6_3:55153865-55153884 Msa0954490:CDS 50.0%
TACCCAAGGCAGATTTGGGT+AGG - chr6_3:55153871-55153890 None:intergenic 50.0%
GTGGATACCCAAGGCAGATT+TGG - chr6_3:55153876-55153895 None:intergenic 50.0%
CCTTCCATTTGAGCTTGTGG+TGG - chr6_3:55153895-55153914 None:intergenic 50.0%
CCACCACAAGCTCAAATGGA+AGG + chr6_3:55153892-55153911 Msa0954490:CDS 50.0%
AGAATCGTCTGTGCGTGTCA+TGG + chr6_3:55153957-55153976 Msa0954490:CDS 50.0%
TGGTTCTCACATGACTCCTG+AGG + chr6_3:55153977-55153996 Msa0954490:CDS 50.0%
!!! GTTTTGGGAAGCTTGCCGAA+TGG + chr6_3:55154050-55154069 Msa0954490:CDS 50.0%
!! TCGCTGACTCAAGCGCCAAT+GGG + chr6_3:55152906-55152925 Msa0954490:five_prime_UTR 55.0%
GCACCAAGCCCTCAAATACC+TGG + chr6_3:55153064-55153083 Msa0954490:CDS 55.0%
! GCACGTTCACCGGTTGTAGT+TGG - chr6_3:55153106-55153125 None:intergenic 55.0%
ACTACAACCGGTGAACGTGC+AGG + chr6_3:55153106-55153125 Msa0954490:CDS 55.0%
TTGAGCTCCTGCACGTTCAC+CGG - chr6_3:55153116-55153135 None:intergenic 55.0%
CATCCTTCCTTTGCAGGGAG+AGG + chr6_3:55153235-55153254 Msa0954490:CDS 55.0%
CTTGCGGCCATAATGCAGTC+AGG - chr6_3:55153395-55153414 None:intergenic 55.0%
TCTAGCGGCCTGCCTTCATA+CGG - chr6_3:55153654-55153673 None:intergenic 55.0%
AGGCCGCTAGAACGAACCAA+AGG + chr6_3:55153663-55153682 Msa0954490:CDS 55.0%
GGAAGCAGCACAAACCTCAG+GGG + chr6_3:55153744-55153763 Msa0954490:CDS 55.0%
CATTAAGCCAGGGCTTACCG+CGG + chr6_3:55153821-55153840 Msa0954490:CDS 55.0%
ACCGCGGATGTGAAATTCGG+AGG + chr6_3:55153837-55153856 Msa0954490:CDS 55.0%
!! GAGCTTGTGGTGGATACCCA+AGG - chr6_3:55153885-55153904 None:intergenic 55.0%
!! TCGCTGACTCAAGCGCCAAT+GGG + chr6_3:55152906-55152925 Msa0954490:five_prime_UTR 55.0%
GCACCAAGCCCTCAAATACC+TGG + chr6_3:55153064-55153083 Msa0954490:CDS 55.0%
! GCACGTTCACCGGTTGTAGT+TGG - chr6_3:55153106-55153125 None:intergenic 55.0%
ACTACAACCGGTGAACGTGC+AGG + chr6_3:55153106-55153125 Msa0954490:CDS 55.0%
TTGAGCTCCTGCACGTTCAC+CGG - chr6_3:55153116-55153135 None:intergenic 55.0%
CATCCTTCCTTTGCAGGGAG+AGG + chr6_3:55153235-55153254 Msa0954490:CDS 55.0%
CTTGCGGCCATAATGCAGTC+AGG - chr6_3:55153395-55153414 None:intergenic 55.0%
TCTAGCGGCCTGCCTTCATA+CGG - chr6_3:55153654-55153673 None:intergenic 55.0%
AGGCCGCTAGAACGAACCAA+AGG + chr6_3:55153663-55153682 Msa0954490:CDS 55.0%
GGAAGCAGCACAAACCTCAG+GGG + chr6_3:55153744-55153763 Msa0954490:CDS 55.0%
CATTAAGCCAGGGCTTACCG+CGG + chr6_3:55153821-55153840 Msa0954490:CDS 55.0%
ACCGCGGATGTGAAATTCGG+AGG + chr6_3:55153837-55153856 Msa0954490:CDS 55.0%
!! GAGCTTGTGGTGGATACCCA+AGG - chr6_3:55153885-55153904 None:intergenic 55.0%
!! GTCGCTGACTCAAGCGCCAA+TGG + chr6_3:55152905-55152924 Msa0954490:five_prime_UTR 60.0%
TCCGAGGCCATGCCGTATGA+AGG + chr6_3:55153639-55153658 Msa0954490:CDS 60.0%
GCCTTCATACGGCATGGCCT+CGG - chr6_3:55153643-55153662 None:intergenic 60.0%
AGGCCATGCCGTATGAAGGC+AGG + chr6_3:55153643-55153662 Msa0954490:CDS 60.0%
CTGGTGCATCTTTGCCCCTG+AGG - chr6_3:55153761-55153780 None:intergenic 60.0%
TTCACATCCGCGGTAAGCCC+TGG - chr6_3:55153831-55153850 None:intergenic 60.0%
!! GTCGCTGACTCAAGCGCCAA+TGG + chr6_3:55152905-55152924 Msa0954490:five_prime_UTR 60.0%
TCCGAGGCCATGCCGTATGA+AGG + chr6_3:55153639-55153658 Msa0954490:CDS 60.0%
GCCTTCATACGGCATGGCCT+CGG - chr6_3:55153643-55153662 None:intergenic 60.0%
AGGCCATGCCGTATGAAGGC+AGG + chr6_3:55153643-55153662 Msa0954490:CDS 60.0%
CTGGTGCATCTTTGCCCCTG+AGG - chr6_3:55153761-55153780 None:intergenic 60.0%
TTCACATCCGCGGTAAGCCC+TGG - chr6_3:55153831-55153850 None:intergenic 60.0%
GGGCCTCTCCCTGCAAAGGA+AGG - chr6_3:55153241-55153260 None:intergenic 65.0%
AGCTGGGCCTCTCCCTGCAA+AGG - chr6_3:55153245-55153264 None:intergenic 65.0%
GGGAGAGGCCCAGCTAACTC+AGG + chr6_3:55153250-55153269 Msa0954490:CDS 65.0%
CGGCCTGCCTTCATACGGCA+TGG - chr6_3:55153649-55153668 None:intergenic 65.0%
GGGCCTCTCCCTGCAAAGGA+AGG - chr6_3:55153241-55153260 None:intergenic 65.0%
AGCTGGGCCTCTCCCTGCAA+AGG - chr6_3:55153245-55153264 None:intergenic 65.0%
GGGAGAGGCCCAGCTAACTC+AGG + chr6_3:55153250-55153269 Msa0954490:CDS 65.0%
CGGCCTGCCTTCATACGGCA+TGG - chr6_3:55153649-55153668 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 55152850 55154343 55152850 ID=Msa0954490;Name=Msa0954490
chr6_3 mRNA 55152850 55154343 55152850 ID=Msa0954490-mRNA-1;Parent=Msa0954490;Name=Msa0954490-mRNA-1;_AED=0.01;_eAED=0.01;_QI=127|1|1|1|1|1|2|243|259
chr6_3 exon 55152850 55153271 55152850 ID=Msa0954490-mRNA-1:exon:11341;Parent=Msa0954490-mRNA-1
chr6_3 exon 55153616 55154343 55153616 ID=Msa0954490-mRNA-1:exon:11342;Parent=Msa0954490-mRNA-1
chr6_3 five_prime_UTR 55152850 55152976 55152850 ID=Msa0954490-mRNA-1:five_prime_utr;Parent=Msa0954490-mRNA-1
chr6_3 CDS 55152977 55153271 55152977 ID=Msa0954490-mRNA-1:cds;Parent=Msa0954490-mRNA-1
chr6_3 CDS 55153616 55154100 55153616 ID=Msa0954490-mRNA-1:cds;Parent=Msa0954490-mRNA-1
chr6_3 three_prime_UTR 55154101 55154343 55154101 ID=Msa0954490-mRNA-1:three_prime_utr;Parent=Msa0954490-mRNA-1
Gene Sequence

>Msa0954490

ATGACTTATGGTGCTCCTTTTTCTGCTCAACAAGTGAATATGATGAGCGGACCTTACACTTCGGCAAAAGAGTCAAACTCTGTTGGGGCACCAAGCCCTCAAATACCTGGAGTGATGCATATGATGCCAACTACAACCGGTGAACGTGCAGGAGCTCAATCTGTGAGTAATGGAAGCTTGCCGATGATGTTTGGACATCCGTATGTTCAGCTTCCTATGTTGGACAAGGATAGCTCATGGGGACGCCCTCAACAGTTTCATCCTTCCTTTGCAGGGAGAGGCCCAGCTAACTCAGCTGCGTTACATCTAAACAATATATCCGAGGCCATGCCGTATGAAGGCAGGCCGCTAGAACGAACCAAAGGTGATGGGAAACAGCGTGTCACCGAAGAAGGCTCCTCTTCACAACCTGAAGACGTGAAAGGAAGCAGCACAAACCTCAGGGGCAAAGATGCACCAGAACAGTCGAAAGGTGAAGGTTCCACCATTGATTTTTCAAACATTAAGCCAGGGCTTACCGCGGATGTGAAATTCGGAGGAAGTGGTTCCTACCCAAATCTGCCTTGGGTATCCACCACAAGCTCAAATGGAAGGACAATATCAGGTGTTACTTACAGGTACAACACTAACCAAATCAGAATCGTCTGTGCGTGTCATGGTTCTCACATGACTCCTGAGGATTTTGTTCGTCATGCAAATGACGAACAAACCAATTCAGATAGCAATTCGGTTTTGGGAAGCTTGCCGAATGGTAATCTTGGTTCATCTTCTCACAGTTAG
Protein sequence

>Msa0954490

MTYGAPFSAQQVNMMSGPYTSAKESNSVGAPSPQIPGVMHMMPTTTGERAGAQSVSNGSLPMMFGHPYVQLPMLDKDSSWGRPQQFHPSFAGRGPANSAALHLNNISEAMPYEGRPLERTKGDGKQRVTEEGSSSQPEDVKGSSTNLRGKDAPEQSKGEGSTIDFSNIKPGLTADVKFGGSGSYPNLPWVSTTSSNGRTISGVTYRYNTNQIRIVCACHGSHMTPEDFVRHANDEQTNSDSNSVLGSLPNGNLGSSSHS*