AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0959870


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0959870 MtrunA17_Chr3g0131571 83.077 65 2 2 22 77 95 159 1.54e-19 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0959870 AT5G51010.1 56.522 69 21 2 17 76 85 153 1.44e-17 72.0

Find 16 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGGAGTTGTAGTTCTTGC+AGG 0.375454 6_3:+77576343 Msa0959870:CDS
ACTCCCACGGCAACTGCAAT+AGG 0.395621 6_3:-77576328 None:intergenic
TTTGTTGACATCTGTTGCAT+AGG 0.431914 6_3:-77576121 None:intergenic
TAGGGCTCAAACCTTCGTTT+AGG 0.438783 6_3:-77576102 None:intergenic
TTGTTGACATCTGTTGCATA+GGG 0.445845 6_3:-77576120 None:intergenic
CTTAGTTCATCGGGCTCCAT+CGG 0.496160 6_3:-77573392 None:intergenic
ACAACTGCTCTTAGTTCATC+GGG 0.500188 6_3:-77573401 None:intergenic
GACAACTGCTCTTAGTTCAT+CGG 0.519655 6_3:-77573402 None:intergenic
ACTTCCTATTGCAGTTGCCG+TGG 0.541016 6_3:+77576324 Msa0959870:CDS
CTTCCTATTGCAGTTGCCGT+GGG 0.547663 6_3:+77576325 Msa0959870:CDS
ATGTTAGAAAGGCGTTAGAA+AGG 0.548907 6_3:+77576160 Msa0959870:CDS
AAATGAAACAGATGTTAGAA+AGG 0.550334 6_3:+77576149 Msa0959870:CDS
GCCGAAAATGGTATCACCGA+TGG 0.562882 6_3:+77573376 None:intergenic
CATGAATGTCAAAATTCTGA+AGG 0.564210 6_3:-77573424 None:intergenic
TGCAAGAACTACAACTCCCA+CGG 0.635141 6_3:-77576341 None:intergenic
TTTGTGGTGCTCCTAAACGA+AGG 0.648339 6_3:+77576091 Msa0959870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGACTTTTGATTATTATATT+AGG + chr6_3:77573662-77573681 Msa0959870:intron 15.0%
!!! TGGTATTTTATTTTATGAAT+TGG + chr6_3:77573989-77574008 Msa0959870:intron 15.0%
!!! ATTTTTAAGGGATTTAATAT+CGG + chr6_3:77574089-77574108 Msa0959870:intron 15.0%
!!! AAATTTGTTATTGAAACAAT+TGG + chr6_3:77574347-77574366 Msa0959870:intron 15.0%
!! AATATATAGATAAATTGCAT+AGG + chr6_3:77574416-77574435 Msa0959870:intron 15.0%
!! AAAATAGCATAAACAAATAT+CGG + chr6_3:77574818-77574837 Msa0959870:intron 15.0%
!! TAGATTAAAACAAAAAGATA+TGG - chr6_3:77574853-77574872 None:intergenic 15.0%
!! TAATATTATACTGATAAGAA+AGG + chr6_3:77575985-77576004 Msa0959870:intron 15.0%
!!! TTACTTTTTTGTAGTTCTAA+AGG + chr6_3:77573470-77573489 Msa0959870:intron 20.0%
!! AAATACGATAAAAATAGATG+AGG - chr6_3:77573507-77573526 None:intergenic 20.0%
!!! TATCGTATTTCTTATTTTAC+AGG + chr6_3:77573517-77573536 Msa0959870:intron 20.0%
!! GATTATTATATTAGGTAACT+TGG + chr6_3:77573670-77573689 Msa0959870:intron 20.0%
!! AATTTACGATCATAAAATTG+TGG - chr6_3:77573916-77573935 None:intergenic 20.0%
!! TTATTTGATGTAACTATCAT+TGG + chr6_3:77573938-77573957 Msa0959870:intron 20.0%
!!! TTTATTTTATGAATTGGTCT+CGG + chr6_3:77573995-77574014 Msa0959870:intron 20.0%
!! GTCATAAATTAATTGCATTT+CGG + chr6_3:77574062-77574081 Msa0959870:intron 20.0%
!!! TTTCTGAAAATTTTGATTTG+CGG + chr6_3:77574161-77574180 Msa0959870:intron 20.0%
!! TTTGTGATTTAATATCCTTT+AGG + chr6_3:77574273-77574292 Msa0959870:intron 20.0%
!!! TGATTATTCTTTTATCACAT+TGG + chr6_3:77574706-77574725 Msa0959870:intron 20.0%
!! TAACTTTATCATATTTGTCA+AGG + chr6_3:77575237-77575256 Msa0959870:intron 20.0%
!! TGGTAAGATATATATTTAGT+TGG + chr6_3:77575646-77575665 Msa0959870:intron 20.0%
!!! TATATATTTAGTTGGTTTTG+TGG + chr6_3:77575654-77575673 Msa0959870:intron 20.0%
!! ATATTAGACCTTAATAAACT+TGG - chr6_3:77575971-77575990 None:intergenic 20.0%
!! ATGTCAAAAACAGAAATAAT+TGG - chr6_3:77576274-77576293 None:intergenic 20.0%
!!! GTATTTCTTATTTTACAGGT+AGG + chr6_3:77573521-77573540 Msa0959870:intron 25.0%
! TAAACTTCAAATATGACACA+TGG + chr6_3:77573567-77573586 Msa0959870:intron 25.0%
!! TCAACAACTTTTAAGCTTTT+GGG - chr6_3:77573600-77573619 None:intergenic 25.0%
!! TTCAACAACTTTTAAGCTTT+TGG - chr6_3:77573601-77573620 None:intergenic 25.0%
! AAGCTTAAAAGTTGTTGAAA+AGG + chr6_3:77573602-77573621 Msa0959870:intron 25.0%
! AGCAAATTTCTCACAAAATT+CGG - chr6_3:77573864-77573883 None:intergenic 25.0%
!!! ATTTTGTGAGAAATTTGCTA+TGG + chr6_3:77573865-77573884 Msa0959870:intron 25.0%
!!! TTTTGTGAGAAATTTGCTAT+GGG + chr6_3:77573866-77573885 Msa0959870:intron 25.0%
!!! AATGTTAGTAATGTTTTGAC+AGG + chr6_3:77573890-77573909 Msa0959870:intron 25.0%
! CACATAAAAAAAAATGTCAC+TGG + chr6_3:77574205-77574224 Msa0959870:intron 25.0%
!!! TATATTTTAAAAACCTCCGT+AGG - chr6_3:77574402-77574421 None:intergenic 25.0%
! TTATTCAATTCGTTTCCATT+AGG + chr6_3:77574630-77574649 Msa0959870:intron 25.0%
! TTTGATCAACTTAACTTTCT+CGG - chr6_3:77574679-77574698 None:intergenic 25.0%
! AAATTTGTCACAAAAGAGAA+AGG - chr6_3:77574774-77574793 None:intergenic 25.0%
! TATACAACAACACATACAAA+TGG - chr6_3:77574876-77574895 None:intergenic 25.0%
!! GTTTTGCTTAAGATGTTAAA+AGG + chr6_3:77574951-77574970 Msa0959870:intron 25.0%
! AAAAATCTGACACAAATATG+TGG - chr6_3:77575051-77575070 None:intergenic 25.0%
! ACAAACAAAGTTGTGTTTAA+AGG - chr6_3:77575084-77575103 None:intergenic 25.0%
!! TTTTAATATGCAGGTTACTT+TGG - chr6_3:77575120-77575139 None:intergenic 25.0%
!!! ATAAGCAAGTTTTAATATGC+AGG - chr6_3:77575129-77575148 None:intergenic 25.0%
!! CATATGAGTTTGTTTAATAC+TGG - chr6_3:77575174-77575193 None:intergenic 25.0%
! AGCATACATTTCAAACTTAT+CGG - chr6_3:77575283-77575302 None:intergenic 25.0%
! TTAATGTGTTACAATAATGC+TGG + chr6_3:77575362-77575381 Msa0959870:intron 25.0%
! AAAATGTATTGCCTAAATTG+TGG - chr6_3:77575858-77575877 None:intergenic 25.0%
! TTAGAGTTTCGATAAAATCA+TGG + chr6_3:77575876-77575895 Msa0959870:intron 25.0%
! GAAATGAGAATCTTTATGAT+TGG - chr6_3:77575940-77575959 None:intergenic 25.0%
! TTTCTCTACCAAGTTTATTA+AGG + chr6_3:77575960-77575979 Msa0959870:intron 25.0%
! TACTGATAAGAAAGGAAAAA+TGG + chr6_3:77575993-77576012 Msa0959870:intron 25.0%
! ACTGATAAGAAAGGAAAAAT+GGG + chr6_3:77575994-77576013 Msa0959870:intron 25.0%
! TTGTAAGAAGCAAAAGAAAT+AGG + chr6_3:77576022-77576041 Msa0959870:intron 25.0%
! TGTAAGAAGCAAAAGAAATA+GGG + chr6_3:77576023-77576042 Msa0959870:intron 25.0%
! AAATGAAACAGATGTTAGAA+AGG + chr6_3:77576149-77576168 Msa0959870:CDS 25.0%
! ATGCAAAAAGAAAATGACTA+TGG - chr6_3:77576232-77576251 None:intergenic 25.0%
!!! TTTATTATGCTGATTTCAAC+AGG + chr6_3:77576296-77576315 Msa0959870:intron 25.0%
CATGAATGTCAAAATTCTGA+AGG - chr6_3:77573427-77573446 None:intergenic 30.0%
!! CTTCAGAATTTTGACATTCA+TGG + chr6_3:77573425-77573444 Msa0959870:CDS 30.0%
!! GAATTTTGACATTCATGGTA+AGG + chr6_3:77573430-77573449 Msa0959870:intron 30.0%
! AAGTAAGTACGATTTTCACT+GGG - chr6_3:77573456-77573475 None:intergenic 30.0%
! AAAGTAAGTACGATTTTCAC+TGG - chr6_3:77573457-77573476 None:intergenic 30.0%
TTACAGGTAGGTGTTTATTA+AGG + chr6_3:77573533-77573552 Msa0959870:intron 30.0%
! AGTCTAACATTGATGATAAG+TGG - chr6_3:77573647-77573666 None:intergenic 30.0%
CATTGGAATCTTATATTGTG+TGG + chr6_3:77573955-77573974 Msa0959870:intron 30.0%
TATAAATGTTGCAGCTAGAT+TGG + chr6_3:77574040-77574059 Msa0959870:intron 30.0%
! CATTTCGGTTGCATTTTTAA+GGG + chr6_3:77574077-77574096 Msa0959870:intron 30.0%
!! ATTGCATAGGTTATTTCTAC+TGG + chr6_3:77574429-77574448 Msa0959870:intron 30.0%
ACACTACATTTAGACACATA+TGG - chr6_3:77574481-77574500 None:intergenic 30.0%
TTGCCAGACTAAAATAATAG+TGG + chr6_3:77574536-77574555 Msa0959870:intron 30.0%
!!! CATCCACTATTATTTTAGTC+TGG - chr6_3:77574542-77574561 None:intergenic 30.0%
TAAGATGTTAAAAGGTCCAT+AGG + chr6_3:77574959-77574978 Msa0959870:intron 30.0%
AAGATGTTAAAAGGTCCATA+GGG + chr6_3:77574960-77574979 Msa0959870:intron 30.0%
AAATCAGACATCTTACGTAT+TGG - chr6_3:77575214-77575233 None:intergenic 30.0%
AATACGTAAGATGTCTGATT+TGG + chr6_3:77575213-77575232 Msa0959870:intron 30.0%
CTCAATTTCATCAAGTCATT+AGG - chr6_3:77575410-77575429 None:intergenic 30.0%
!!! TCTGTTTCTATTCCGTTTTA+TGG + chr6_3:77575487-77575506 Msa0959870:intron 30.0%
! ATTAACTGTCATGGATTACT+TGG + chr6_3:77575745-77575764 Msa0959870:intron 30.0%
! TTAACTGTCATGGATTACTT+GGG + chr6_3:77575746-77575765 Msa0959870:intron 30.0%
TCAAATTCTAAGTGCATGTT+AGG + chr6_3:77575789-77575808 Msa0959870:intron 30.0%
CACAATTGTTACCACAATTT+AGG + chr6_3:77575844-77575863 Msa0959870:intron 30.0%
CTCTTAATTTCTGAATTTGG+TGG - chr6_3:77575904-77575923 None:intergenic 30.0%
CTGCTCTTAATTTCTGAATT+TGG - chr6_3:77575907-77575926 None:intergenic 30.0%
GTAAGAAGCAAAAGAAATAG+GGG + chr6_3:77576024-77576043 Msa0959870:intron 30.0%
GCATTATGATTGTCTGAAAA+TGG + chr6_3:77576058-77576077 Msa0959870:intron 30.0%
! CTAACATTGATGATAAGTGG+CGG - chr6_3:77573644-77573663 None:intergenic 35.0%
! GCATTTCGGTTGCATTTTTA+AGG + chr6_3:77574076-77574095 Msa0959870:intron 35.0%
TCTTGATTGGATCTTAGTCA+TGG + chr6_3:77574120-77574139 Msa0959870:intron 35.0%
ATCTTAGTCATGGAAGCTAA+TGG + chr6_3:77574130-77574149 Msa0959870:intron 35.0%
!!! AAATTTTGATTTGCGGCTGT+TGG + chr6_3:77574168-77574187 Msa0959870:intron 35.0%
CGAAGAAGACATAAACCTAA+AGG - chr6_3:77574291-77574310 None:intergenic 35.0%
CAATTGGCAAATCATCTACA+AGG + chr6_3:77574363-77574382 Msa0959870:intron 35.0%
AGGATAATGAACACAACCTA+CGG + chr6_3:77574383-77574402 Msa0959870:intron 35.0%
TCCATTAGGTACCTAACAAA+TGG + chr6_3:77574644-77574663 Msa0959870:intron 35.0%
! ACCATTTGTTAGGTACCTAA+TGG - chr6_3:77574648-77574667 None:intergenic 35.0%
GGAGAAGAATACCATTTGTT+AGG - chr6_3:77574658-77574677 None:intergenic 35.0%
GCATAAACAAATATCGGACA+TGG + chr6_3:77574824-77574843 Msa0959870:intron 35.0%
TTGTTGTATATTCCCTGCAT+TGG + chr6_3:77574886-77574905 Msa0959870:intron 35.0%
!! TGTGTCAGATTTTTGTTTGC+AGG + chr6_3:77575057-77575076 Msa0959870:intron 35.0%
AGACATCTTACGTATTGGAA+TGG - chr6_3:77575209-77575228 None:intergenic 35.0%
ATGTGTTACAATAATGCTGG+TGG + chr6_3:77575365-77575384 Msa0959870:intron 35.0%
TGTGTTACAATAATGCTGGT+GGG + chr6_3:77575366-77575385 Msa0959870:intron 35.0%
GAAATTGAGAACATTTCACG+TGG + chr6_3:77575421-77575440 Msa0959870:intron 35.0%
ATTGTGTGGTGAAACTTTGT+GGG - chr6_3:77575585-77575604 None:intergenic 35.0%
AATTGTGTGGTGAAACTTTG+TGG - chr6_3:77575586-77575605 None:intergenic 35.0%
GTTTCACCACACAATTGTTA+AGG + chr6_3:77575590-77575609 Msa0959870:intron 35.0%
TCAACTCCTTAACAATTGTG+TGG - chr6_3:77575599-77575618 None:intergenic 35.0%
!!! TGATGTGGTTTTTGGCAATT+TGG + chr6_3:77575626-77575645 Msa0959870:intron 35.0%
CGAAACAGAATTAACTGTCA+TGG + chr6_3:77575736-77575755 Msa0959870:intron 35.0%
! AAATGGTGTTTTGCAGTTTG+TGG + chr6_3:77576075-77576094 Msa0959870:intron 35.0%
TTGTTGACATCTGTTGCATA+GGG - chr6_3:77576123-77576142 None:intergenic 35.0%
TTTGTTGACATCTGTTGCAT+AGG - chr6_3:77576124-77576143 None:intergenic 35.0%
!! ATGTTAGAAAGGCGTTAGAA+AGG + chr6_3:77576160-77576179 Msa0959870:CDS 35.0%
TCCAAAGAGATGAAGCAATT+GGG + chr6_3:77576198-77576217 Msa0959870:intron 35.0%
ACAACTGCTCTTAGTTCATC+GGG - chr6_3:77573404-77573423 None:intergenic 40.0%
GACAACTGCTCTTAGTTCAT+CGG - chr6_3:77573405-77573424 None:intergenic 40.0%
ATTGTGTGGATCAGAGTCAT+TGG + chr6_3:77573969-77573988 Msa0959870:intron 40.0%
ATCGGCTAGAACTTCTTGAT+TGG + chr6_3:77574107-77574126 Msa0959870:intron 40.0%
ATAATGAACACAACCTACGG+AGG + chr6_3:77574386-77574405 Msa0959870:intron 40.0%
GCACAAAAACATCATCCCTA+TGG - chr6_3:77574978-77574997 None:intergenic 40.0%
!! TAGGGATGATGTTTTTGTGC+TGG + chr6_3:77574978-77574997 Msa0959870:intron 40.0%
TGGTGTAATGTGTGAGTAGA+GGG + chr6_3:77574998-77575017 Msa0959870:intron 40.0%
GTGTTACAATAATGCTGGTG+GGG + chr6_3:77575367-77575386 Msa0959870:intron 40.0%
GTGCTGCAAAAGCCATAAAA+CGG - chr6_3:77575502-77575521 None:intergenic 40.0%
!! AAGAGAGTAGAAGCACCATT+TGG - chr6_3:77575558-77575577 None:intergenic 40.0%
! ACAACCTCTGATGTGGTTTT+TGG + chr6_3:77575618-77575637 Msa0959870:intron 40.0%
ATTGCCAAAAACCACATCAG+AGG - chr6_3:77575625-77575644 None:intergenic 40.0%
ATGTCTTTCAGAAGCTGTCT+TGG + chr6_3:77575680-77575699 Msa0959870:intron 40.0%
TTTCAGAAGCTGTCTTGGTT+TGG + chr6_3:77575685-77575704 Msa0959870:intron 40.0%
GTTAATTCTGTTTCGTCAGC+AGG - chr6_3:77575731-77575750 None:intergenic 40.0%
GTTAGGTTCAGCGAATTTGA+TGG + chr6_3:77575806-77575825 Msa0959870:intron 40.0%
CTCCAAAGAGATGAAGCAAT+TGG + chr6_3:77576197-77576216 Msa0959870:intron 40.0%
ACCCAATTGCTTCATCTCTT+TGG - chr6_3:77576202-77576221 None:intergenic 40.0%
ATTTGCGGCTGTTGGCATAA+AGG + chr6_3:77574176-77574195 Msa0959870:intron 45.0%
ACTGGACTCTCAGACTTTGA+AGG + chr6_3:77574223-77574242 Msa0959870:intron 45.0%
CTGGTGTAATGTGTGAGTAG+AGG + chr6_3:77574997-77575016 Msa0959870:intron 45.0%
TTTGTGGTGCTCCTAAACGA+AGG + chr6_3:77576091-77576110 Msa0959870:CDS 45.0%
TAGGGCTCAAACCTTCGTTT+AGG - chr6_3:77576105-77576124 None:intergenic 45.0%
TGCAAGAACTACAACTCCCA+CGG - chr6_3:77576344-77576363 None:intergenic 45.0%
!!! ATATAATAAACATTAAATTT+TGG - chr6_3:77573801-77573820 None:intergenic 5.0%
CTTAGTTCATCGGGCTCCAT+CGG - chr6_3:77573395-77573414 None:intergenic 50.0%
CATTGGATGCAGCTCATGAG+CGG + chr6_3:77574723-77574742 Msa0959870:intron 50.0%
GAGCGGAGTTTGAACACGTA+AGG + chr6_3:77574740-77574759 Msa0959870:intron 50.0%
TAACATCAGCAGAGCAGCCA+TGG - chr6_3:77574915-77574934 None:intergenic 50.0%
ATTTGGACTGCTGGAGCAGT+CGG - chr6_3:77575541-77575560 None:intergenic 50.0%
!! AGAAGCACCATTTGGACTGC+TGG - chr6_3:77575550-77575569 None:intergenic 50.0%
GGAGTTGACAACCTCTGATG+TGG + chr6_3:77575611-77575630 Msa0959870:intron 50.0%
ACTTCCTATTGCAGTTGCCG+TGG + chr6_3:77576324-77576343 Msa0959870:CDS 50.0%
CTTCCTATTGCAGTTGCCGT+GGG + chr6_3:77576325-77576344 Msa0959870:CDS 50.0%
GTGGGAGTTGTAGTTCTTGC+AGG + chr6_3:77576343-77576362 Msa0959870:CDS 50.0%
!! ATTCCCTGCATTGGCTGCCA+TGG + chr6_3:77574895-77574914 Msa0959870:intron 55.0%
TGCTGGTGGGGTTGTAAGCA+AGG + chr6_3:77575379-77575398 Msa0959870:intron 55.0%
GACTGCTCCAGCAGTCCAAA+TGG + chr6_3:77575540-77575559 Msa0959870:intron 55.0%
ACTCCCACGGCAACTGCAAT+AGG - chr6_3:77576331-77576350 None:intergenic 55.0%
CAGCCATGGCAGCCAATGCA+GGG - chr6_3:77574901-77574920 None:intergenic 60.0%
GCAGCCATGGCAGCCAATGC+AGG - chr6_3:77574902-77574921 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 77573383 77576396 77573383 ID=Msa0959870;Name=Msa0959870
chr6_3 mRNA 77573383 77576396 77573383 ID=Msa0959870-mRNA-1;Parent=Msa0959870;Name=Msa0959870-mRNA-1;_AED=0.53;_eAED=0.85;_QI=0|0|0|0.66|1|1|3|0|77
chr6_3 exon 77573383 77573446 77573383 ID=Msa0959870-mRNA-1:exon:13888;Parent=Msa0959870-mRNA-1
chr6_3 exon 77576091 77576181 77576091 ID=Msa0959870-mRNA-1:exon:13889;Parent=Msa0959870-mRNA-1
chr6_3 exon 77576318 77576396 77576318 ID=Msa0959870-mRNA-1:exon:13890;Parent=Msa0959870-mRNA-1
chr6_3 CDS 77573383 77573446 77573383 ID=Msa0959870-mRNA-1:cds;Parent=Msa0959870-mRNA-1
chr6_3 CDS 77576091 77576181 77576091 ID=Msa0959870-mRNA-1:cds;Parent=Msa0959870-mRNA-1
chr6_3 CDS 77576318 77576396 77576318 ID=Msa0959870-mRNA-1:cds;Parent=Msa0959870-mRNA-1
Gene Sequence

>Msa0959870

ATGGTATCACCGATGGAGCCCGATGAACTAAGAGCAGTTGTCCTTCAGAATTTTGACATTCATGTTTGTGGTGCTCCTAAACGAAGGTTTGAGCCCTATGCAACAGATGTCAACAAAAAAGCAAATGAAACAGATGTTAGAAAGGCGTTAGAAAGGAAAGCACTTCCTATTGCAGTTGCCGTGGGAGTTGTAGTTCTTGCAGGATTATACTTCTATCTCAATAGCACATTTTAG

Protein sequence

>Msa0959870

MVSPMEPDELRAVVLQNFDIHVCGAPKRRFEPYATDVNKKANETDVRKALERKALPIAVAVGVVVLAGLYFYLNSTF*