Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0962100 | XP_003620175.1 | 94.393 | 214 | 11 | 1 | 1 | 214 | 1 | 213 | 6.96e-132 | 379 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0962100 | sp|Q9LMU2|KTI5_ARATH | 43.578 | 218 | 96 | 8 | 1 | 214 | 1 | 195 | 1.59e-47 | 157 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0962100 | Q6ISX8 | 94.393 | 214 | 11 | 1 | 1 | 214 | 1 | 213 | 3.33e-132 | 379 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0390420 | Msa0962100 | 0.863893 | 1.797642e-64 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0962100 | MtrunA17_Chr6g0481961 | 94.393 | 214 | 11 | 1 | 1 | 214 | 1 | 213 | 6.40e-136 | 379 |
| Msa0962100 | MtrunA17_Chr6g0481951 | 93.925 | 214 | 12 | 1 | 1 | 214 | 1 | 213 | 9.30e-135 | 376 |
| Msa0962100 | MtrunA17_Chr6g0482041 | 58.605 | 215 | 85 | 4 | 1 | 214 | 1 | 212 | 3.94e-81 | 241 |
| Msa0962100 | MtrunA17_Chr6g0482031 | 61.000 | 200 | 71 | 4 | 18 | 214 | 17 | 212 | 6.73e-81 | 240 |
| Msa0962100 | MtrunA17_Chr6g0482011 | 56.122 | 196 | 77 | 2 | 16 | 211 | 15 | 201 | 1.04e-71 | 216 |
| Msa0962100 | MtrunA17_Chr6g0482021 | 47.706 | 218 | 91 | 6 | 1 | 214 | 1 | 199 | 3.63e-57 | 179 |
| Msa0962100 | MtrunA17_Chr7g0228581 | 48.663 | 187 | 84 | 4 | 29 | 214 | 27 | 202 | 1.37e-55 | 176 |
| Msa0962100 | MtrunA17_Chr6g0481911 | 40.359 | 223 | 104 | 10 | 1 | 214 | 1 | 203 | 2.22e-40 | 137 |
| Msa0962100 | MtrunA17_Chr6g0481941 | 40.265 | 226 | 102 | 11 | 1 | 214 | 1 | 205 | 2.24e-39 | 134 |
| Msa0962100 | MtrunA17_Chr3g0137441 | 29.545 | 220 | 123 | 7 | 6 | 214 | 1 | 199 | 1.66e-20 | 85.5 |
| Msa0962100 | MtrunA17_Chr3g0137421 | 31.390 | 223 | 113 | 9 | 6 | 214 | 1 | 197 | 1.43e-18 | 80.5 |
| Msa0962100 | MtrunA17_Chr3g0137411 | 32.589 | 224 | 114 | 11 | 1 | 213 | 1 | 198 | 2.63e-16 | 74.3 |
| Msa0962100 | MtrunA17_Chr2g0314441 | 32.589 | 224 | 115 | 11 | 1 | 214 | 1 | 198 | 9.16e-16 | 72.8 |
| Msa0962100 | MtrunA17_Chr8g0359111 | 29.384 | 211 | 127 | 8 | 10 | 213 | 6 | 201 | 2.21e-14 | 68.9 |
| Msa0962100 | MtrunA17_Chr6g0476921 | 30.612 | 196 | 111 | 11 | 26 | 213 | 23 | 201 | 4.26e-14 | 68.6 |
| Msa0962100 | MtrunA17_Chr2g0314431 | 31.308 | 214 | 121 | 10 | 5 | 214 | 8 | 199 | 1.08e-13 | 67.0 |
| Msa0962100 | MtrunA17_Chr6g0452521 | 30.244 | 205 | 118 | 8 | 6 | 205 | 41 | 225 | 2.10e-12 | 64.3 |
| Msa0962100 | MtrunA17_Chr6g0476961 | 28.959 | 221 | 123 | 11 | 1 | 214 | 1 | 194 | 3.03e-12 | 63.2 |
| Msa0962100 | MtrunA17_Chr8g0359161 | 28.421 | 190 | 114 | 8 | 31 | 213 | 29 | 203 | 8.90e-12 | 62.0 |
| Msa0962100 | MtrunA17_Chr6g0452491 | 31.220 | 205 | 115 | 9 | 6 | 205 | 9 | 192 | 1.29e-11 | 61.6 |
| Msa0962100 | MtrunA17_Chr6g0476951 | 28.507 | 221 | 125 | 10 | 1 | 214 | 1 | 195 | 4.51e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0962100 | AT1G17860.1 | 43.578 | 218 | 96 | 8 | 1 | 214 | 1 | 195 | 1.62e-48 | 157 |
| Msa0962100 | AT1G73260.1 | 36.000 | 200 | 110 | 6 | 21 | 214 | 18 | 205 | 1.36e-28 | 107 |
| Msa0962100 | AT1G73325.1 | 33.636 | 220 | 123 | 9 | 1 | 214 | 1 | 203 | 3.16e-25 | 98.6 |
Find 52 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTTGAACCAATTATTAA+TGG | 0.190104 | 6_3:-84939214 | None:intergenic |
| ACACGAATAACACCCTTCTT+AGG | 0.207105 | 6_3:-84939048 | None:intergenic |
| TTGAGCACACAACCACTTCT+TGG | 0.247520 | 6_3:+84938793 | Msa0962100:CDS |
| GATGGGAAACCATTAATAAT+TGG | 0.274442 | 6_3:+84939205 | Msa0962100:CDS |
| GGAGTGTTTGTTGATGAAAA+TGG | 0.277827 | 6_3:+84939315 | Msa0962100:CDS |
| CACCTGTGAATGGGTATAAC+CGG | 0.336556 | 6_3:-84938895 | None:intergenic |
| AGACAATGGTTTATTACTAT+TGG | 0.339490 | 6_3:+84939162 | Msa0962100:CDS |
| TGTTGAAATTTAACTTTAAG+AGG | 0.341900 | 6_3:-84939366 | None:intergenic |
| ACACCCTTCTTAGGGTTAAC+AGG | 0.353495 | 6_3:-84939039 | None:intergenic |
| ATTCACAGGTGTGGACCTTA+TGG | 0.370571 | 6_3:+84938907 | Msa0962100:CDS |
| TGAAAATGGGAATAAACGTT+TGG | 0.383026 | 6_3:+84939329 | Msa0962100:CDS |
| TGGTTCAAGATTGAGAAGTA+TGG | 0.396705 | 6_3:+84939225 | Msa0962100:CDS |
| TGGTGGTGTTGTTGGAAATC+CGG | 0.404788 | 6_3:+84939182 | Msa0962100:CDS |
| GAGTGTTTGTTGATGAAAAT+GGG | 0.410318 | 6_3:+84939316 | Msa0962100:CDS |
| ATTACTATTGGTGGTGTTGT+TGG | 0.423355 | 6_3:+84939174 | Msa0962100:CDS |
| GAACAAGTGGTTGACACATT+AGG | 0.434007 | 6_3:+84938835 | Msa0962100:CDS |
| TATGCCTGTTAACCCTAAGA+AGG | 0.446168 | 6_3:+84939035 | Msa0962100:CDS |
| CACGAATAACACCCTTCTTA+GGG | 0.454923 | 6_3:-84939047 | None:intergenic |
| CTACTTCTACATTTACCATA+AGG | 0.458135 | 6_3:-84938922 | None:intergenic |
| TTGAAAGACTGCACCACACT+AGG | 0.459638 | 6_3:-84939273 | None:intergenic |
| AGTCTTGCCCTAGCAAGTAT+TGG | 0.463122 | 6_3:+84938952 | Msa0962100:CDS |
| GTCTTTCAAGCATATGTGTA+AGG | 0.466167 | 6_3:+84939287 | Msa0962100:CDS |
| AACTTGCCCTCTTGATGTTG+TGG | 0.473079 | 6_3:+84938978 | Msa0962100:CDS |
| CAATGGTTTATTACTATTGG+TGG | 0.475905 | 6_3:+84939165 | Msa0962100:CDS |
| TTATTAATGGTTTCCCATCC+CGG | 0.501459 | 6_3:-84939201 | None:intergenic |
| AAGCATATGTGTAAGGATGT+TGG | 0.504444 | 6_3:+84939294 | Msa0962100:CDS |
| ATAAGGTCCACACCTGTGAA+TGG | 0.514083 | 6_3:-84938905 | None:intergenic |
| TAAGGTCCACACCTGTGAAT+GGG | 0.519817 | 6_3:-84938904 | None:intergenic |
| TGCTAATTACTATATTATCC+CGG | 0.540548 | 6_3:+84938876 | Msa0962100:CDS |
| GGTGGTGTTGTTGGAAATCC+GGG | 0.542963 | 6_3:+84939183 | Msa0962100:CDS |
| GCTTCAGCTGCTCCAAGAAG+TGG | 0.551993 | 6_3:-84938805 | None:intergenic |
| TCCCGGTTATACCCATTCAC+AGG | 0.554778 | 6_3:+84938893 | Msa0962100:CDS |
| ATGCCTGTTAACCCTAAGAA+GGG | 0.557642 | 6_3:+84939036 | Msa0962100:CDS |
| GTGTTGTTGGAAATCCGGGA+TGG | 0.564391 | 6_3:+84939187 | Msa0962100:CDS |
| TGTTGTTGGAAATCCGGGAT+GGG | 0.569448 | 6_3:+84939188 | Msa0962100:CDS |
| TGAAAGACTGCACCACACTA+GGG | 0.577137 | 6_3:-84939272 | None:intergenic |
| TTAACAGGCATAAAAGTAAG+TGG | 0.579627 | 6_3:-84939024 | None:intergenic |
| TATGGTAAATGTAGAAGTAG+TGG | 0.581114 | 6_3:+84938925 | Msa0962100:CDS |
| CAAGCTTCCACACCATGGAA+TGG | 0.584005 | 6_3:-84939119 | None:intergenic |
| TGAAGCTTCAAATGAACAAG+TGG | 0.587694 | 6_3:+84938822 | Msa0962100:CDS |
| AAGTGGTTGTGTGCTCAAGA+AGG | 0.588756 | 6_3:-84938788 | None:intergenic |
| GTCTACACCATTCCATGGTG+TGG | 0.596900 | 6_3:+84939112 | Msa0962100:CDS |
| ACCTGTGAATGGGTATAACC+GGG | 0.598899 | 6_3:-84938894 | None:intergenic |
| AACTTGTCTACACCATTCCA+TGG | 0.601053 | 6_3:+84939107 | Msa0962100:CDS |
| TTAATGTATCTAAGAGACAA+TGG | 0.603832 | 6_3:+84939148 | Msa0962100:CDS |
| TCAACAACCACAACATCAAG+AGG | 0.610607 | 6_3:-84938985 | None:intergenic |
| TGCTCAAGAAGGCAAAGAGA+AGG | 0.611541 | 6_3:-84938777 | None:intergenic |
| GTATGGTGATGATTATAAGT+TGG | 0.617624 | 6_3:+84939242 | Msa0962100:CDS |
| TATAGTAATTAGCATCAGCT+CGG | 0.621190 | 6_3:-84938867 | None:intergenic |
| GTTATACCCATTCACAGGTG+TGG | 0.621916 | 6_3:+84938898 | Msa0962100:CDS |
| ACGATCAAGCTTCCACACCA+TGG | 0.629046 | 6_3:-84939124 | None:intergenic |
| CAACAACCACAACATCAAGA+GGG | 0.678487 | 6_3:-84938984 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCTTGAACCAATTATTAA+TGG | - | chr6_3:84939217-84939236 | None:intergenic | 20.0% |
| !!! | TGTTGAAATTTAACTTTAAG+AGG | - | chr6_3:84939369-84939388 | None:intergenic | 20.0% |
| ! | TGCTAATTACTATATTATCC+CGG | + | chr6_3:84938876-84938895 | Msa0962100:CDS | 25.0% |
| ! | TTAATGTATCTAAGAGACAA+TGG | + | chr6_3:84939148-84939167 | Msa0962100:CDS | 25.0% |
| ! | AGACAATGGTTTATTACTAT+TGG | + | chr6_3:84939162-84939181 | Msa0962100:CDS | 25.0% |
| TATAGTAATTAGCATCAGCT+CGG | - | chr6_3:84938870-84938889 | None:intergenic | 30.0% | |
| CTACTTCTACATTTACCATA+AGG | - | chr6_3:84938925-84938944 | None:intergenic | 30.0% | |
| TATGGTAAATGTAGAAGTAG+TGG | + | chr6_3:84938925-84938944 | Msa0962100:CDS | 30.0% | |
| !! | AGTTTTTCCAATACTTGCTA+GGG | - | chr6_3:84938962-84938981 | None:intergenic | 30.0% |
| ! | AAGTTTTTCCAATACTTGCT+AGG | - | chr6_3:84938963-84938982 | None:intergenic | 30.0% |
| TTAACAGGCATAAAAGTAAG+TGG | - | chr6_3:84939027-84939046 | None:intergenic | 30.0% | |
| CAATGGTTTATTACTATTGG+TGG | + | chr6_3:84939165-84939184 | Msa0962100:CDS | 30.0% | |
| GATGGGAAACCATTAATAAT+TGG | + | chr6_3:84939205-84939224 | Msa0962100:CDS | 30.0% | |
| ! | GTATGGTGATGATTATAAGT+TGG | + | chr6_3:84939242-84939261 | Msa0962100:CDS | 30.0% |
| ! | GAGTGTTTGTTGATGAAAAT+GGG | + | chr6_3:84939316-84939335 | Msa0962100:CDS | 30.0% |
| TGAAAATGGGAATAAACGTT+TGG | + | chr6_3:84939329-84939348 | Msa0962100:CDS | 30.0% | |
| TGAAGCTTCAAATGAACAAG+TGG | + | chr6_3:84938822-84938841 | Msa0962100:CDS | 35.0% | |
| !! | ATTACTATTGGTGGTGTTGT+TGG | + | chr6_3:84939174-84939193 | Msa0962100:CDS | 35.0% |
| TTATTAATGGTTTCCCATCC+CGG | - | chr6_3:84939204-84939223 | None:intergenic | 35.0% | |
| TGGTTCAAGATTGAGAAGTA+TGG | + | chr6_3:84939225-84939244 | Msa0962100:CDS | 35.0% | |
| GTCTTTCAAGCATATGTGTA+AGG | + | chr6_3:84939287-84939306 | Msa0962100:CDS | 35.0% | |
| AAGCATATGTGTAAGGATGT+TGG | + | chr6_3:84939294-84939313 | Msa0962100:CDS | 35.0% | |
| ! | GGAGTGTTTGTTGATGAAAA+TGG | + | chr6_3:84939315-84939334 | Msa0962100:CDS | 35.0% |
| GAACAAGTGGTTGACACATT+AGG | + | chr6_3:84938835-84938854 | Msa0962100:CDS | 40.0% | |
| CAACAACCACAACATCAAGA+GGG | - | chr6_3:84938987-84939006 | None:intergenic | 40.0% | |
| TCAACAACCACAACATCAAG+AGG | - | chr6_3:84938988-84939007 | None:intergenic | 40.0% | |
| TATGCCTGTTAACCCTAAGA+AGG | + | chr6_3:84939035-84939054 | Msa0962100:CDS | 40.0% | |
| ATGCCTGTTAACCCTAAGAA+GGG | + | chr6_3:84939036-84939055 | Msa0962100:CDS | 40.0% | |
| CACGAATAACACCCTTCTTA+GGG | - | chr6_3:84939050-84939069 | None:intergenic | 40.0% | |
| ACACGAATAACACCCTTCTT+AGG | - | chr6_3:84939051-84939070 | None:intergenic | 40.0% | |
| AACTTGTCTACACCATTCCA+TGG | + | chr6_3:84939107-84939126 | Msa0962100:CDS | 40.0% | |
| TGCTCAAGAAGGCAAAGAGA+AGG | - | chr6_3:84938780-84938799 | None:intergenic | 45.0% | |
| AAGTGGTTGTGTGCTCAAGA+AGG | - | chr6_3:84938791-84938810 | None:intergenic | 45.0% | |
| TTGAGCACACAACCACTTCT+TGG | + | chr6_3:84938793-84938812 | Msa0962100:CDS | 45.0% | |
| ACCTGTGAATGGGTATAACC+GGG | - | chr6_3:84938897-84938916 | None:intergenic | 45.0% | |
| CACCTGTGAATGGGTATAAC+CGG | - | chr6_3:84938898-84938917 | None:intergenic | 45.0% | |
| GTTATACCCATTCACAGGTG+TGG | + | chr6_3:84938898-84938917 | Msa0962100:CDS | 45.0% | |
| TAAGGTCCACACCTGTGAAT+GGG | - | chr6_3:84938907-84938926 | None:intergenic | 45.0% | |
| ATAAGGTCCACACCTGTGAA+TGG | - | chr6_3:84938908-84938927 | None:intergenic | 45.0% | |
| ATTCACAGGTGTGGACCTTA+TGG | + | chr6_3:84938907-84938926 | Msa0962100:CDS | 45.0% | |
| AGTCTTGCCCTAGCAAGTAT+TGG | + | chr6_3:84938952-84938971 | Msa0962100:CDS | 45.0% | |
| AACTTGCCCTCTTGATGTTG+TGG | + | chr6_3:84938978-84938997 | Msa0962100:CDS | 45.0% | |
| ACACCCTTCTTAGGGTTAAC+AGG | - | chr6_3:84939042-84939061 | None:intergenic | 45.0% | |
| !! | TGGTGGTGTTGTTGGAAATC+CGG | + | chr6_3:84939182-84939201 | Msa0962100:CDS | 45.0% |
| TGTTGTTGGAAATCCGGGAT+GGG | + | chr6_3:84939188-84939207 | Msa0962100:CDS | 45.0% | |
| TGAAAGACTGCACCACACTA+GGG | - | chr6_3:84939275-84939294 | None:intergenic | 45.0% | |
| TTGAAAGACTGCACCACACT+AGG | - | chr6_3:84939276-84939295 | None:intergenic | 45.0% | |
| TCCCGGTTATACCCATTCAC+AGG | + | chr6_3:84938893-84938912 | Msa0962100:CDS | 50.0% | |
| GTCTACACCATTCCATGGTG+TGG | + | chr6_3:84939112-84939131 | Msa0962100:CDS | 50.0% | |
| CAAGCTTCCACACCATGGAA+TGG | - | chr6_3:84939122-84939141 | None:intergenic | 50.0% | |
| ACGATCAAGCTTCCACACCA+TGG | - | chr6_3:84939127-84939146 | None:intergenic | 50.0% | |
| !! | GGTGGTGTTGTTGGAAATCC+GGG | + | chr6_3:84939183-84939202 | Msa0962100:CDS | 50.0% |
| ! | GTGTTGTTGGAAATCCGGGA+TGG | + | chr6_3:84939187-84939206 | Msa0962100:CDS | 50.0% |
| !! | GTTGGTGTTTTGCCCTAGTG+TGG | + | chr6_3:84939260-84939279 | Msa0962100:CDS | 50.0% |
| GCTTCAGCTGCTCCAAGAAG+TGG | - | chr6_3:84938808-84938827 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 84938751 | 84939395 | 84938751 | ID=Msa0962100;Name=Msa0962100 |
| chr6_3 | mRNA | 84938751 | 84939395 | 84938751 | ID=Msa0962100-mRNA-1;Parent=Msa0962100;Name=Msa0962100-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|214 |
| chr6_3 | exon | 84938751 | 84939395 | 84938751 | ID=Msa0962100-mRNA-1:exon:15232;Parent=Msa0962100-mRNA-1 |
| chr6_3 | CDS | 84938751 | 84939395 | 84938751 | ID=Msa0962100-mRNA-1:cds;Parent=Msa0962100-mRNA-1 |
| Gene Sequence |
| Protein sequence |
