AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0962440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0962440 MtrunA17_Chr6g0482261 93.827 243 15 0 20 262 1 243 1.57e-171 473
Msa0962440 MtrunA17_Chr6g0482111 48.571 245 116 2 25 260 1231 1474 2.88e-62 211
Msa0962440 MtrunA17_Chr6g0482091 36.364 242 137 5 27 255 172 409 1.54e-35 131
Msa0962440 MtrunA17_Chr3g0081531 32.500 240 151 2 27 256 1155 1393 2.64e-29 116
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCATTTCTCTTCCTTTC+TGG 0.034051 6_3:+85708378 None:intergenic
TTATAACAAATTCGTGTTAT+TGG 0.204130 6_3:-85708076 Msa0962440:three_prime_UTR
TATTGTATTCCGTTCTAAAT+TGG 0.228037 6_3:-85707975 Msa0962440:three_prime_UTR
TTGTCGTTAATAATTGAGTA+TGG 0.273310 6_3:-85708040 Msa0962440:three_prime_UTR
AATAACACGAATTTGTTATA+AGG 0.274106 6_3:+85708078 None:intergenic
ATTGTGTTTGTCAAATAAAT+TGG 0.275884 6_3:-85708516 Msa0962440:CDS
AAATAAATTGGTTTGCTTGC+AGG 0.329157 6_3:-85708504 Msa0962440:CDS
TTGTAATATTACGTGGTTCA+TGG 0.361309 6_3:+85709848 None:intergenic
GCTGAAAACTGAGCAGGATT+TGG 0.376883 6_3:-85708321 Msa0962440:CDS
GAGAGTTTCTTTCAGCTGTA+TGG 0.383707 6_3:+85708754 None:intergenic
ACACTTTAATATCAGCATCT+AGG 0.389508 6_3:+85708447 None:intergenic
CACTTTAATATCAGCATCTA+GGG 0.397286 6_3:+85708448 None:intergenic
GAATGATACAACATAGGAAT+AGG 0.421689 6_3:+85708135 None:intergenic
AATGAATTGTTCTCGAAGCT+TGG 0.428695 6_3:+85708262 None:intergenic
AAGTACTTTCCCGCAGGAAT+CGG 0.451888 6_3:+85708177 None:intergenic
GAACAATTCATTCGAAGTAC+TGG 0.454128 6_3:-85708251 Msa0962440:CDS
TATTTACCGGCACAGGTGCG+GGG 0.470289 6_3:-85709004 Msa0962440:intron
TGTAATATTACGTGGTTCAT+GGG 0.482200 6_3:+85709849 None:intergenic
CTCAACTTGCCACAAAATGA+AGG 0.486414 6_3:-85708722 Msa0962440:CDS
TTGGAGTATCACAATGCATC+AGG 0.489446 6_3:-85708638 Msa0962440:CDS
TAACAACAAAATCAGAAATA+CGG 0.490640 6_3:-85708691 Msa0962440:CDS
GTTGATTCTCCGATTCCTGC+GGG 0.491224 6_3:-85708186 Msa0962440:three_prime_UTR
ATTAACGACAAATTAATTCG+AGG 0.492087 6_3:+85708052 None:intergenic
CATGTTTCGTAAGAGAAAGA+TGG 0.506791 6_3:-85708408 Msa0962440:CDS
TGAAACATGGAGATTAATAC+AGG 0.511596 6_3:+85708102 None:intergenic
TAAATCTTGGAGAATATGAA+TGG 0.512242 6_3:+85708817 None:intergenic
GAAAACTGAGCAGGATTTGG+TGG 0.523634 6_3:-85708318 Msa0962440:CDS
TTCAGCTGTATGGAACGTCC+TGG 0.551931 6_3:+85708764 None:intergenic
ATTTACCGGCACAGGTGCGG+GGG 0.552133 6_3:-85709003 Msa0962440:intron
TCCAAGGCTGAAAACTGAGC+AGG 0.553538 6_3:-85708327 Msa0962440:CDS
GACTATTCAAGAGCTAAAGA+TGG 0.553841 6_3:-85708960 Msa0962440:CDS
TTCAAAGATACTGCATGTGA+TGG 0.554419 6_3:+85708863 None:intergenic
TGTTGATTCTCCGATTCCTG+CGG 0.557812 6_3:-85708187 Msa0962440:three_prime_UTR
AAGATGGAATTGTACCAGAA+AGG 0.561211 6_3:-85708392 Msa0962440:CDS
ATTCATTCACCAATTTAGAA+CGG 0.561522 6_3:+85707966 None:intergenic
ATAGGAATAGGATAAAACTT+AGG 0.562179 6_3:+85708147 None:intergenic
TTACTAAAGTACTTTCCCGC+AGG 0.576905 6_3:+85708171 None:intergenic
AACACGTGATAATATTGAAG+CGG 0.591614 6_3:-85708291 Msa0962440:CDS
AGAGTTTCATAATATCGAGA+AGG 0.593089 6_3:-85708543 Msa0962440:CDS
ATATAAGAATGATACAACAT+AGG 0.594801 6_3:+85708129 None:intergenic
ATTTGATGATCAATGTTCCA+AGG 0.609761 6_3:-85708343 Msa0962440:CDS
TTTACCGGCACAGGTGCGGG+GGG 0.627096 6_3:-85709002 Msa0962440:intron
AAAACTGAGCAGGATTTGGT+GGG 0.633162 6_3:-85708317 Msa0962440:CDS
GATGGAGAAGTTGTGTCTGA+TGG 0.633487 6_3:+85708881 None:intergenic
TTTGTTATAAGGATGAAACA+TGG 0.655866 6_3:+85708089 None:intergenic
GTGATAATATTGAAGCGGAG+TGG 0.721680 6_3:-85708286 Msa0962440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATACTTATTATAATAATT+AGG - chr6_3:85708251-85708270 Msa0962440:CDS 10.0%
!! AATGATAAAAAAATTAGTAT+CGG + chr6_3:85708415-85708434 None:intergenic 10.0%
!! GTATACTTATTATAATAATT+AGG - chr6_3:85708251-85708270 Msa0962440:CDS 10.0%
!! AATGATAAAAAAATTAGTAT+CGG + chr6_3:85708415-85708434 None:intergenic 10.0%
!!! TTTTTATAGTCTAAATCTTT+AGG - chr6_3:85708126-85708145 Msa0962440:three_prime_UTR 15.0%
!!! TTTTTATAGTCTAAATCTTT+AGG - chr6_3:85708126-85708145 Msa0962440:three_prime_UTR 15.0%
!!! ATCATTATTATTGTTGATAG+AGG - chr6_3:85708327-85708346 Msa0962440:CDS 20.0%
!! ATTCTTCTTAGTAAACATTA+GGG + chr6_3:85708509-85708528 None:intergenic 20.0%
!! TATTCTTCTTAGTAAACATT+AGG + chr6_3:85708510-85708529 None:intergenic 20.0%
!!! AATCTTTTGAGAATAAATCT+TGG + chr6_3:85708998-85709017 None:intergenic 20.0%
!! TAACAACAAAATCAGAAATA+CGG - chr6_3:85709108-85709127 Msa0962440:intron 20.0%
!! ATTGTGTTTGTCAAATAAAT+TGG - chr6_3:85709283-85709302 Msa0962440:intron 20.0%
!! ATATAAGAATGATACAACAT+AGG + chr6_3:85709673-85709692 None:intergenic 20.0%
!!! AATAACACGAATTTGTTATA+AGG + chr6_3:85709724-85709743 None:intergenic 20.0%
!! TTATAACAAATTCGTGTTAT+TGG - chr6_3:85709723-85709742 Msa0962440:intron 20.0%
!!! ATCATTATTATTGTTGATAG+AGG - chr6_3:85708327-85708346 Msa0962440:CDS 20.0%
!! ATTCTTCTTAGTAAACATTA+GGG + chr6_3:85708509-85708528 None:intergenic 20.0%
!! TATTCTTCTTAGTAAACATT+AGG + chr6_3:85708510-85708529 None:intergenic 20.0%
!!! AATCTTTTGAGAATAAATCT+TGG + chr6_3:85708998-85709017 None:intergenic 20.0%
!! TAACAACAAAATCAGAAATA+CGG - chr6_3:85709108-85709127 Msa0962440:intron 20.0%
!! ATTGTGTTTGTCAAATAAAT+TGG - chr6_3:85709283-85709302 Msa0962440:intron 20.0%
!! ATATAAGAATGATACAACAT+AGG + chr6_3:85709673-85709692 None:intergenic 20.0%
!!! AATAACACGAATTTGTTATA+AGG + chr6_3:85709724-85709743 None:intergenic 20.0%
!! TTATAACAAATTCGTGTTAT+TGG - chr6_3:85709723-85709742 Msa0962440:intron 20.0%
! TACTCAAATTGTAGCAAAAA+TGG + chr6_3:85708082-85708101 None:intergenic 25.0%
! ATTCTTCCTAGTAAACATTA+GGG + chr6_3:85708228-85708247 None:intergenic 25.0%
! TATTCTTCCTAGTAAACATT+AGG + chr6_3:85708229-85708248 None:intergenic 25.0%
!!! TTTTATCATTGTCATTTGCT+CGG - chr6_3:85708425-85708444 Msa0962440:CDS 25.0%
! TAAATCTTGGAGAATATGAA+TGG + chr6_3:85708985-85709004 None:intergenic 25.0%
!! AAACAAAATCCTTCATTTTG+TGG + chr6_3:85709089-85709108 None:intergenic 25.0%
! ATAGGAATAGGATAAAACTT+AGG + chr6_3:85709655-85709674 None:intergenic 25.0%
!! TTTGTTATAAGGATGAAACA+TGG + chr6_3:85709713-85709732 None:intergenic 25.0%
! ATTAACGACAAATTAATTCG+AGG + chr6_3:85709750-85709769 None:intergenic 25.0%
! TTGTCGTTAATAATTGAGTA+TGG - chr6_3:85709759-85709778 Msa0962440:intron 25.0%
!! TATTGTATTCCGTTCTAAAT+TGG - chr6_3:85709824-85709843 Msa0962440:intron 25.0%
! ATTCATTCACCAATTTAGAA+CGG + chr6_3:85709836-85709855 None:intergenic 25.0%
! TACTCAAATTGTAGCAAAAA+TGG + chr6_3:85708082-85708101 None:intergenic 25.0%
! ATTCTTCCTAGTAAACATTA+GGG + chr6_3:85708228-85708247 None:intergenic 25.0%
! TATTCTTCCTAGTAAACATT+AGG + chr6_3:85708229-85708248 None:intergenic 25.0%
!!! TTTTATCATTGTCATTTGCT+CGG - chr6_3:85708425-85708444 Msa0962440:CDS 25.0%
! TAAATCTTGGAGAATATGAA+TGG + chr6_3:85708985-85709004 None:intergenic 25.0%
!! AAACAAAATCCTTCATTTTG+TGG + chr6_3:85709089-85709108 None:intergenic 25.0%
! ATAGGAATAGGATAAAACTT+AGG + chr6_3:85709655-85709674 None:intergenic 25.0%
!! TTTGTTATAAGGATGAAACA+TGG + chr6_3:85709713-85709732 None:intergenic 25.0%
! ATTAACGACAAATTAATTCG+AGG + chr6_3:85709750-85709769 None:intergenic 25.0%
! TTGTCGTTAATAATTGAGTA+TGG - chr6_3:85709759-85709778 Msa0962440:intron 25.0%
!! TATTGTATTCCGTTCTAAAT+TGG - chr6_3:85709824-85709843 Msa0962440:intron 25.0%
! ATTCATTCACCAATTTAGAA+CGG + chr6_3:85709836-85709855 None:intergenic 25.0%
GTTGTTCTTGTAATATTACG+TGG + chr6_3:85707961-85707980 None:intergenic 30.0%
AACAATTCTTCTTCTCTCTT+TGG + chr6_3:85708023-85708042 None:intergenic 30.0%
AAAGAGAGAAGAAGAATTGT+TGG - chr6_3:85708022-85708041 Msa0962440:three_prime_UTR 30.0%
AAGAGAGAAGAAGAATTGTT+GGG - chr6_3:85708023-85708042 Msa0962440:three_prime_UTR 30.0%
TTTGCTACAATTTGAGTATG+TGG - chr6_3:85708084-85708103 Msa0962440:three_prime_UTR 30.0%
!! GTCTAAATCTTTAGGTTGTT+TGG - chr6_3:85708134-85708153 Msa0962440:three_prime_UTR 30.0%
ACAATAAAAATCACGTGACT+TGG + chr6_3:85708623-85708642 None:intergenic 30.0%
!! TTTGTGATTGTTATTTGCTC+TGG - chr6_3:85708699-85708718 Msa0962440:CDS 30.0%
!! ATTGTTATTTGCTCTGGAAT+TGG - chr6_3:85708705-85708724 Msa0962440:CDS 30.0%
TTGTTATATCGATCTTGAGT+TGG - chr6_3:85708757-85708776 Msa0962440:CDS 30.0%
!!! GAGAATATGAATGGCTTTTT+TGG + chr6_3:85708976-85708995 None:intergenic 30.0%
!! AACTGTTGCAATGTTTTGAA+CGG - chr6_3:85709132-85709151 Msa0962440:intron 30.0%
AGAGTTTCATAATATCGAGA+AGG - chr6_3:85709256-85709275 Msa0962440:intron 30.0%
!! AAATAAATTGGTTTGCTTGC+AGG - chr6_3:85709295-85709314 Msa0962440:intron 30.0%
CACTTTAATATCAGCATCTA+GGG + chr6_3:85709354-85709373 None:intergenic 30.0%
ACACTTTAATATCAGCATCT+AGG + chr6_3:85709355-85709374 None:intergenic 30.0%
! ATTTGATGATCAATGTTCCA+AGG - chr6_3:85709456-85709475 Msa0962440:intron 30.0%
AACACGTGATAATATTGAAG+CGG - chr6_3:85709508-85709527 Msa0962440:intron 30.0%
GAATGATACAACATAGGAAT+AGG + chr6_3:85709667-85709686 None:intergenic 30.0%
TGAAACATGGAGATTAATAC+AGG + chr6_3:85709700-85709719 None:intergenic 30.0%
TTGTAATATTACGTGGTTCA+TGG + chr6_3:85707954-85707973 None:intergenic 30.0%
GTTGTTCTTGTAATATTACG+TGG + chr6_3:85707961-85707980 None:intergenic 30.0%
AACAATTCTTCTTCTCTCTT+TGG + chr6_3:85708023-85708042 None:intergenic 30.0%
AAAGAGAGAAGAAGAATTGT+TGG - chr6_3:85708022-85708041 Msa0962440:three_prime_UTR 30.0%
AAGAGAGAAGAAGAATTGTT+GGG - chr6_3:85708023-85708042 Msa0962440:three_prime_UTR 30.0%
TTTGCTACAATTTGAGTATG+TGG - chr6_3:85708084-85708103 Msa0962440:three_prime_UTR 30.0%
!! GTCTAAATCTTTAGGTTGTT+TGG - chr6_3:85708134-85708153 Msa0962440:three_prime_UTR 30.0%
ACAATAAAAATCACGTGACT+TGG + chr6_3:85708623-85708642 None:intergenic 30.0%
!! TTTGTGATTGTTATTTGCTC+TGG - chr6_3:85708699-85708718 Msa0962440:CDS 30.0%
!! ATTGTTATTTGCTCTGGAAT+TGG - chr6_3:85708705-85708724 Msa0962440:CDS 30.0%
TTGTTATATCGATCTTGAGT+TGG - chr6_3:85708757-85708776 Msa0962440:CDS 30.0%
!!! GAGAATATGAATGGCTTTTT+TGG + chr6_3:85708976-85708995 None:intergenic 30.0%
!! AACTGTTGCAATGTTTTGAA+CGG - chr6_3:85709132-85709151 Msa0962440:intron 30.0%
AGAGTTTCATAATATCGAGA+AGG - chr6_3:85709256-85709275 Msa0962440:intron 30.0%
!! AAATAAATTGGTTTGCTTGC+AGG - chr6_3:85709295-85709314 Msa0962440:intron 30.0%
CACTTTAATATCAGCATCTA+GGG + chr6_3:85709354-85709373 None:intergenic 30.0%
ACACTTTAATATCAGCATCT+AGG + chr6_3:85709355-85709374 None:intergenic 30.0%
! ATTTGATGATCAATGTTCCA+AGG - chr6_3:85709456-85709475 Msa0962440:intron 30.0%
AACACGTGATAATATTGAAG+CGG - chr6_3:85709508-85709527 Msa0962440:intron 30.0%
GAATGATACAACATAGGAAT+AGG + chr6_3:85709667-85709686 None:intergenic 30.0%
TGAAACATGGAGATTAATAC+AGG + chr6_3:85709700-85709719 None:intergenic 30.0%
TAAATGCACATTCTATGAGC+AGG - chr6_3:85708473-85708492 Msa0962440:CDS 35.0%
! AAAAATCACGTGACTTGGAT+CGG + chr6_3:85708618-85708637 None:intergenic 35.0%
TCACACACAGAATGTTAAAG+TGG + chr6_3:85708666-85708685 None:intergenic 35.0%
GACTATTCAAGAGCTAAAGA+TGG - chr6_3:85708839-85708858 Msa0962440:CDS 35.0%
TTCAAAGATACTGCATGTGA+TGG + chr6_3:85708939-85708958 None:intergenic 35.0%
CATGTTTCGTAAGAGAAAGA+TGG - chr6_3:85709391-85709410 Msa0962440:intron 35.0%
AAGATGGAATTGTACCAGAA+AGG - chr6_3:85709407-85709426 Msa0962440:intron 35.0%
CTTTCATTTCTCTTCCTTTC+TGG + chr6_3:85709424-85709443 None:intergenic 35.0%
AATGAATTGTTCTCGAAGCT+TGG + chr6_3:85709540-85709559 None:intergenic 35.0%
GAACAATTCATTCGAAGTAC+TGG - chr6_3:85709548-85709567 Msa0962440:intron 35.0%
TAAATGCACATTCTATGAGC+AGG - chr6_3:85708473-85708492 Msa0962440:CDS 35.0%
! AAAAATCACGTGACTTGGAT+CGG + chr6_3:85708618-85708637 None:intergenic 35.0%
TCACACACAGAATGTTAAAG+TGG + chr6_3:85708666-85708685 None:intergenic 35.0%
GACTATTCAAGAGCTAAAGA+TGG - chr6_3:85708839-85708858 Msa0962440:CDS 35.0%
TTCAAAGATACTGCATGTGA+TGG + chr6_3:85708939-85708958 None:intergenic 35.0%
CATGTTTCGTAAGAGAAAGA+TGG - chr6_3:85709391-85709410 Msa0962440:intron 35.0%
AAGATGGAATTGTACCAGAA+AGG - chr6_3:85709407-85709426 Msa0962440:intron 35.0%
CTTTCATTTCTCTTCCTTTC+TGG + chr6_3:85709424-85709443 None:intergenic 35.0%
AATGAATTGTTCTCGAAGCT+TGG + chr6_3:85709540-85709559 None:intergenic 35.0%
GAACAATTCATTCGAAGTAC+TGG - chr6_3:85709548-85709567 Msa0962440:intron 35.0%
! ACTACGTATGAAAGAAGCAC+AGG - chr6_3:85707979-85707998 Msa0962440:three_prime_UTR 40.0%
! AAGAAGCACAGGCACATTAA+CGG - chr6_3:85707990-85708009 Msa0962440:three_prime_UTR 40.0%
!! TGTTGGGTTGAAGTAGTAGA+TGG - chr6_3:85708039-85708058 Msa0962440:three_prime_UTR 40.0%
GCAACAAACATCATCAAAGG+TGG + chr6_3:85708204-85708223 None:intergenic 40.0%
GGAGCAACAAACATCATCAA+AGG + chr6_3:85708207-85708226 None:intergenic 40.0%
GTTGCTCCCTAATGTTTACT+AGG - chr6_3:85708219-85708238 Msa0962440:exon 40.0%
GTGATTCTTATTCTCTCACG+TGG - chr6_3:85708357-85708376 Msa0962440:CDS 40.0%
! CATTGTCATTTGCTCGGAAT+TGG - chr6_3:85708431-85708450 Msa0962440:CDS 40.0%
!! TGCTATTGTTGATAGTGACG+TGG - chr6_3:85708566-85708585 Msa0962440:CDS 40.0%
! GTGATTTTTATTGTCGCACG+TGG - chr6_3:85708629-85708648 Msa0962440:CDS 40.0%
! CTGACTGGTTGTCTATTTAC+CGG - chr6_3:85708782-85708801 Msa0962440:CDS 40.0%
!! TTCGCTCTTTTGATTCATCC+AGG - chr6_3:85709017-85709036 Msa0962440:intron 40.0%
GAGAGTTTCTTTCAGCTGTA+TGG + chr6_3:85709048-85709067 None:intergenic 40.0%
! CTTCATTTTGTGGCAAGTTG+AGG + chr6_3:85709079-85709098 None:intergenic 40.0%
CTCAACTTGCCACAAAATGA+AGG - chr6_3:85709077-85709096 Msa0962440:intron 40.0%
!! TGTTGCAATGTTTTGAACGG+TGG - chr6_3:85709135-85709154 Msa0962440:intron 40.0%
!! ATGTTTTGAACGGTGGAGTT+TGG - chr6_3:85709142-85709161 Msa0962440:intron 40.0%
! TTGGAGTATCACAATGCATC+AGG - chr6_3:85709161-85709180 Msa0962440:intron 40.0%
! AAAACTGAGCAGGATTTGGT+GGG - chr6_3:85709482-85709501 Msa0962440:intron 40.0%
GTGATAATATTGAAGCGGAG+TGG - chr6_3:85709513-85709532 Msa0962440:intron 40.0%
! TTACTAAAGTACTTTCCCGC+AGG + chr6_3:85709631-85709650 None:intergenic 40.0%
! ACTACGTATGAAAGAAGCAC+AGG - chr6_3:85707979-85707998 Msa0962440:three_prime_UTR 40.0%
! AAGAAGCACAGGCACATTAA+CGG - chr6_3:85707990-85708009 Msa0962440:three_prime_UTR 40.0%
!! TGTTGGGTTGAAGTAGTAGA+TGG - chr6_3:85708039-85708058 Msa0962440:three_prime_UTR 40.0%
GCAACAAACATCATCAAAGG+TGG + chr6_3:85708204-85708223 None:intergenic 40.0%
GGAGCAACAAACATCATCAA+AGG + chr6_3:85708207-85708226 None:intergenic 40.0%
GTTGCTCCCTAATGTTTACT+AGG - chr6_3:85708219-85708238 Msa0962440:exon 40.0%
GTGATTCTTATTCTCTCACG+TGG - chr6_3:85708357-85708376 Msa0962440:CDS 40.0%
! CATTGTCATTTGCTCGGAAT+TGG - chr6_3:85708431-85708450 Msa0962440:CDS 40.0%
!! TGCTATTGTTGATAGTGACG+TGG - chr6_3:85708566-85708585 Msa0962440:CDS 40.0%
! GTGATTTTTATTGTCGCACG+TGG - chr6_3:85708629-85708648 Msa0962440:CDS 40.0%
! CTGACTGGTTGTCTATTTAC+CGG - chr6_3:85708782-85708801 Msa0962440:CDS 40.0%
!! TTCGCTCTTTTGATTCATCC+AGG - chr6_3:85709017-85709036 Msa0962440:intron 40.0%
GAGAGTTTCTTTCAGCTGTA+TGG + chr6_3:85709048-85709067 None:intergenic 40.0%
! CTTCATTTTGTGGCAAGTTG+AGG + chr6_3:85709079-85709098 None:intergenic 40.0%
CTCAACTTGCCACAAAATGA+AGG - chr6_3:85709077-85709096 Msa0962440:intron 40.0%
!! TGTTGCAATGTTTTGAACGG+TGG - chr6_3:85709135-85709154 Msa0962440:intron 40.0%
!! ATGTTTTGAACGGTGGAGTT+TGG - chr6_3:85709142-85709161 Msa0962440:intron 40.0%
! TTGGAGTATCACAATGCATC+AGG - chr6_3:85709161-85709180 Msa0962440:intron 40.0%
! AAAACTGAGCAGGATTTGGT+GGG - chr6_3:85709482-85709501 Msa0962440:intron 40.0%
GTGATAATATTGAAGCGGAG+TGG - chr6_3:85709513-85709532 Msa0962440:intron 40.0%
! TTACTAAAGTACTTTCCCGC+AGG + chr6_3:85709631-85709650 None:intergenic 40.0%
!! GATCTTGAGTTGGTTCTGAC+TGG - chr6_3:85708767-85708786 Msa0962440:CDS 45.0%
GATGGAGAAGTTGTGTCTGA+TGG + chr6_3:85708921-85708940 None:intergenic 45.0%
!! CATTTTGTGGCAAGTTGAGG+AGG + chr6_3:85709076-85709095 None:intergenic 45.0%
GCTGAAAACTGAGCAGGATT+TGG - chr6_3:85709478-85709497 Msa0962440:intron 45.0%
GAAAACTGAGCAGGATTTGG+TGG - chr6_3:85709481-85709500 Msa0962440:intron 45.0%
!! TGTTGATTCTCCGATTCCTG+CGG - chr6_3:85709612-85709631 Msa0962440:intron 45.0%
AAGTACTTTCCCGCAGGAAT+CGG + chr6_3:85709625-85709644 None:intergenic 45.0%
!! GATCTTGAGTTGGTTCTGAC+TGG - chr6_3:85708767-85708786 Msa0962440:CDS 45.0%
GATGGAGAAGTTGTGTCTGA+TGG + chr6_3:85708921-85708940 None:intergenic 45.0%
!! CATTTTGTGGCAAGTTGAGG+AGG + chr6_3:85709076-85709095 None:intergenic 45.0%
GCTGAAAACTGAGCAGGATT+TGG - chr6_3:85709478-85709497 Msa0962440:intron 45.0%
GAAAACTGAGCAGGATTTGG+TGG - chr6_3:85709481-85709500 Msa0962440:intron 45.0%
!! TGTTGATTCTCCGATTCCTG+CGG - chr6_3:85709612-85709631 Msa0962440:intron 45.0%
AAGTACTTTCCCGCAGGAAT+CGG + chr6_3:85709625-85709644 None:intergenic 45.0%
!! TATACTTATTATAATAATTA+GGG - chr6_3:85708252-85708271 Msa0962440:CDS 5.0%
!! AATAATGATAATAATTATTA+GGG + chr6_3:85708316-85708335 None:intergenic 5.0%
!! TAATAATGATAATAATTATT+AGG + chr6_3:85708317-85708336 None:intergenic 5.0%
!!! ATTTTTTTTTTTTTTAAACT+TGG - chr6_3:85708589-85708608 Msa0962440:CDS 5.0%
!! TATACTTATTATAATAATTA+GGG - chr6_3:85708252-85708271 Msa0962440:CDS 5.0%
!! AATAATGATAATAATTATTA+GGG + chr6_3:85708316-85708335 None:intergenic 5.0%
!! TAATAATGATAATAATTATT+AGG + chr6_3:85708317-85708336 None:intergenic 5.0%
!!! ATTTTTTTTTTTTTTAAACT+TGG - chr6_3:85708589-85708608 Msa0962440:CDS 5.0%
AAGCACAGGCACATTAACGG+CGG - chr6_3:85707993-85708012 Msa0962440:three_prime_UTR 50.0%
!! GCTCTGGAATTGGTTCTGAC+TGG - chr6_3:85708715-85708734 Msa0962440:CDS 50.0%
GGTTGTCTATTTACCGGCAC+AGG - chr6_3:85708788-85708807 Msa0962440:CDS 50.0%
TCTATTTACCGGCACAGGTG+CGG - chr6_3:85708793-85708812 Msa0962440:CDS 50.0%
TTCAGCTGTATGGAACGTCC+TGG + chr6_3:85709038-85709057 None:intergenic 50.0%
TCCAAGGCTGAAAACTGAGC+AGG - chr6_3:85709472-85709491 Msa0962440:intron 50.0%
! TCCTGCTCAGTTTTCAGCCT+TGG + chr6_3:85709476-85709495 None:intergenic 50.0%
! GTTGATTCTCCGATTCCTGC+GGG - chr6_3:85709613-85709632 Msa0962440:intron 50.0%
AAGCACAGGCACATTAACGG+CGG - chr6_3:85707993-85708012 Msa0962440:three_prime_UTR 50.0%
!! GCTCTGGAATTGGTTCTGAC+TGG - chr6_3:85708715-85708734 Msa0962440:CDS 50.0%
GGTTGTCTATTTACCGGCAC+AGG - chr6_3:85708788-85708807 Msa0962440:CDS 50.0%
TCTATTTACCGGCACAGGTG+CGG - chr6_3:85708793-85708812 Msa0962440:CDS 50.0%
TTCAGCTGTATGGAACGTCC+TGG + chr6_3:85709038-85709057 None:intergenic 50.0%
TCCAAGGCTGAAAACTGAGC+AGG - chr6_3:85709472-85709491 Msa0962440:intron 50.0%
! TCCTGCTCAGTTTTCAGCCT+TGG + chr6_3:85709476-85709495 None:intergenic 50.0%
! GTTGATTCTCCGATTCCTGC+GGG - chr6_3:85709613-85709632 Msa0962440:intron 50.0%
CTATTTACCGGCACAGGTGC+GGG - chr6_3:85708794-85708813 Msa0962440:CDS 55.0%
TATTTACCGGCACAGGTGCG+GGG - chr6_3:85708795-85708814 Msa0962440:CDS 55.0%
CTATTTACCGGCACAGGTGC+GGG - chr6_3:85708794-85708813 Msa0962440:CDS 55.0%
TATTTACCGGCACAGGTGCG+GGG - chr6_3:85708795-85708814 Msa0962440:CDS 55.0%
ATTTACCGGCACAGGTGCGG+GGG - chr6_3:85708796-85708815 Msa0962440:CDS 60.0%
ATTTACCGGCACAGGTGCGG+GGG - chr6_3:85708796-85708815 Msa0962440:CDS 60.0%
TTTACCGGCACAGGTGCGGG+GGG - chr6_3:85708797-85708816 Msa0962440:CDS 65.0%
ATTTCCCCCCGCACCTGTGC+CGG + chr6_3:85708804-85708823 None:intergenic 65.0%
TTTACCGGCACAGGTGCGGG+GGG - chr6_3:85708797-85708816 Msa0962440:CDS 65.0%
ATTTCCCCCCGCACCTGTGC+CGG + chr6_3:85708804-85708823 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 85707953 85709868 85707953 ID=Msa0962440;Name=Msa0962440
chr6_3 mRNA 85707953 85709868 85707953 ID=Msa0962440-mRNA-1;Parent=Msa0962440;Name=Msa0962440-mRNA-1;_AED=0.20;_eAED=0.21;_QI=0|0|0.5|1|0|0.5|2|279|262
chr6_3 exon 85707953 85709011 85707953 ID=Msa0962440-mRNA-1:exon:15432;Parent=Msa0962440-mRNA-1
chr6_3 exon 85709860 85709868 85709860 ID=Msa0962440-mRNA-1:exon:15431;Parent=Msa0962440-mRNA-1
chr6_3 CDS 85709860 85709868 85709860 ID=Msa0962440-mRNA-1:cds;Parent=Msa0962440-mRNA-1
chr6_3 CDS 85708232 85709011 85708232 ID=Msa0962440-mRNA-1:cds;Parent=Msa0962440-mRNA-1
chr6_3 three_prime_UTR 85707953 85708231 85707953 ID=Msa0962440-mRNA-1:three_prime_utr;Parent=Msa0962440-mRNA-1
Gene Sequence

>Msa0962440

ATGAACCACGTGCGGGGGGAAATATTTCGCATTCAAGAGACTATTCAAGAGCTAAAGATGGTAAATCACGACACGCAAAATGAAACTGATATGAGAGCATTTTATGCATCTCTAAAACCATCAGACACAACTTCTCCATCACATGCAGTATCTTTGAAAAGTAGTGAAAACACCAAAAAAGCCATTCATATTCTCCAAGATTTATTCTCAAAAGATTTCGCTCTTTTGATTCATCCAGGACGTTCCATACAGCTGAAAGAAACTCTCAGATACCTCCTCAACTTGCCACAAAATGAAGGATTTTGTTTAACAACAAAATCAGAAATACGGAAACTGTTGCAATGTTTTGAACGGTGGAGTTTGGAGTATCACAATGCATCAGGTCTTTCAGCAGCTGCAGAAACTGAATTATCAAAAGCAAGTGAAGTGATGAATGATCTCGACGCTAATGTTCAAGAGTTTCATAATATCGAGAAGGAAGAATTGTGTTTGTCAAATAAATTGGTTTGCTTGCAGGAAGAGAAGAGAATGCTTGAAGAGAAAATTAAAACCCTAGATGCTGATATTAAAGTGTCTACAAAAAGAAGAGACATGTTTCGTAAGAGAAAGATGGAATTGTACCAGAAAGGAAGAGAAATGAAAGCAAAAAGAGATGATTTGATGATCAATGTTCCAAGGCTGAAAACTGAGCAGGATTTGGTGGGTAAAACACGTGATAATATTGAAGCGGAGTGGTCCAAGCTTCGAGAACAATTCATTCGAAGTACTGGTATTAAAGAATTGATCTAA
Protein sequence

>Msa0962440

MNHVRGEIFRIQETIQELKMVNHDTQNETDMRAFYASLKPSDTTSPSHAVSLKSSENTKKAIHILQDLFSKDFALLIHPGRSIQLKETLRYLLNLPQNEGFCLTTKSEIRKLLQCFERWSLEYHNASGLSAAAETELSKASEVMNDLDANVQEFHNIEKEELCLSNKLVCLQEEKRMLEEKIKTLDADIKVSTKRRDMFRKRKMELYQKGREMKAKRDDLMINVPRLKTEQDLVGKTRDNIEAEWSKLREQFIRSTGIKELI*