Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0970780 | XP_003618157.1 | 98.198 | 111 | 2 | 0 | 1 | 111 | 1 | 111 | 1.41e-72 | 221 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0970780 | sp|Q29Q81|SMR6_ARATH | 56.034 | 116 | 36 | 7 | 1 | 106 | 1 | 111 | 4.31e-23 | 89.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0970780 | G7KQ10 | 98.198 | 111 | 2 | 0 | 1 | 111 | 1 | 111 | 6.73e-73 | 221 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0795870 | Msa0970780 | 0.801524 | 8.646926e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0970780 | MtrunA17_Chr6g0449711 | 98.198 | 111 | 2 | 0 | 1 | 111 | 1 | 111 | 1.30e-76 | 221 |
| Msa0970780 | MtrunA17_Chr8g0368031 | 44.348 | 115 | 59 | 4 | 1 | 110 | 1 | 115 | 2.02e-20 | 79.3 |
| Msa0970780 | MtrunA17_Chr5g0412851 | 42.202 | 109 | 59 | 2 | 7 | 111 | 5 | 113 | 5.77e-17 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0970780 | AT5G40460.1 | 56.034 | 116 | 36 | 7 | 1 | 106 | 1 | 111 | 4.38e-24 | 89.0 |
| Msa0970780 | AT1G60783.1 | 48.624 | 109 | 48 | 5 | 1 | 106 | 1 | 104 | 6.64e-20 | 78.2 |
| Msa0970780 | AT1G10690.1 | 45.872 | 109 | 54 | 4 | 1 | 106 | 1 | 107 | 4.78e-18 | 73.6 |
Find 20 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGCTCATCTTTGTGTTTAT+AGG | 0.129879 | 6_4:-2429654 | None:intergenic |
| TTGAACACTGTTTCCAAATC+AGG | 0.345104 | 6_4:-2429840 | None:intergenic |
| AACACAAATGGAGAAGAGTT+TGG | 0.385335 | 6_4:+2429578 | Msa0970780:CDS |
| TGGAATCTGAAGCTAAGAAA+TGG | 0.392466 | 6_4:+2429598 | Msa0970780:CDS |
| GCTAAGAAATGGGTAATTGC+TGG | 0.408525 | 6_4:+2429609 | Msa0970780:CDS |
| TGATTTGGAAACAGTGTTCA+AGG | 0.433713 | 6_4:+2429842 | Msa0970780:CDS |
| TCAAAGTGTAACAATGTTGT+TGG | 0.464863 | 6_4:+2429795 | Msa0970780:CDS |
| TTCTTCTTCCTTGGTGCCGG+TGG | 0.474580 | 6_4:-2429768 | None:intergenic |
| GGAATCTGAAGCTAAGAAAT+GGG | 0.495322 | 6_4:+2429599 | Msa0970780:CDS |
| GAATTCGAAGAAAACACAAA+TGG | 0.515083 | 6_4:+2429566 | Msa0970780:CDS |
| TAATTGCTGGAATTTCCCTC+AGG | 0.537646 | 6_4:+2429622 | Msa0970780:CDS |
| TATAGGTTTCAATGACCTGA+GGG | 0.548525 | 6_4:-2429637 | None:intergenic |
| GGTGAACATGTCGTGTCCAC+CGG | 0.573845 | 6_4:+2429752 | Msa0970780:CDS |
| GGCAAAAGAAGCTAGGATAC+CGG | 0.578164 | 6_4:+2429731 | Msa0970780:CDS |
| GGTGGACACGACATGTTCAC+CGG | 0.588547 | 6_4:-2429750 | None:intergenic |
| AAGTGTAACAATGTTGTTGG+TGG | 0.601001 | 6_4:+2429798 | Msa0970780:CDS |
| CACCAACGGCAAAAGAAGCT+AGG | 0.614440 | 6_4:+2429724 | Msa0970780:CDS |
| AGAAAATTCAACTACACCAA+CGG | 0.629624 | 6_4:+2429710 | Msa0970780:CDS |
| TTATAGGTTTCAATGACCTG+AGG | 0.630083 | 6_4:-2429638 | None:intergenic |
| TGTCGTGTCCACCGGCACCA+AGG | 0.645896 | 6_4:+2429760 | Msa0970780:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTGAAATTTTCTTCTTCCT+TGG | - | chr6_4:2429780-2429799 | None:intergenic | 25.0% |
| GAATTCGAAGAAAACACAAA+TGG | + | chr6_4:2429566-2429585 | Msa0970780:CDS | 30.0% | |
| ACAAAGATGAGCACAAAAAA+CGG | + | chr6_4:2429663-2429682 | Msa0970780:CDS | 30.0% | |
| AGAAAATTCAACTACACCAA+CGG | + | chr6_4:2429710-2429729 | Msa0970780:CDS | 30.0% | |
| TCAAAGTGTAACAATGTTGT+TGG | + | chr6_4:2429795-2429814 | Msa0970780:CDS | 30.0% | |
| AACACAAATGGAGAAGAGTT+TGG | + | chr6_4:2429578-2429597 | Msa0970780:CDS | 35.0% | |
| ! | TGGAATCTGAAGCTAAGAAA+TGG | + | chr6_4:2429598-2429617 | Msa0970780:CDS | 35.0% |
| ! | GGAATCTGAAGCTAAGAAAT+GGG | + | chr6_4:2429599-2429618 | Msa0970780:CDS | 35.0% |
| TATAGGTTTCAATGACCTGA+GGG | - | chr6_4:2429640-2429659 | None:intergenic | 35.0% | |
| TTATAGGTTTCAATGACCTG+AGG | - | chr6_4:2429641-2429660 | None:intergenic | 35.0% | |
| GTGCTCATCTTTGTGTTTAT+AGG | - | chr6_4:2429657-2429676 | None:intergenic | 35.0% | |
| AAGTGTAACAATGTTGTTGG+TGG | + | chr6_4:2429798-2429817 | Msa0970780:CDS | 35.0% | |
| !!! | GTTTTTCAAGTGTCCTGATT+TGG | + | chr6_4:2429827-2429846 | Msa0970780:CDS | 35.0% |
| TTGAACACTGTTTCCAAATC+AGG | - | chr6_4:2429843-2429862 | None:intergenic | 35.0% | |
| ! | TGATTTGGAAACAGTGTTCA+AGG | + | chr6_4:2429842-2429861 | Msa0970780:CDS | 35.0% |
| GCTAAGAAATGGGTAATTGC+TGG | + | chr6_4:2429609-2429628 | Msa0970780:CDS | 40.0% | |
| TAATTGCTGGAATTTCCCTC+AGG | + | chr6_4:2429622-2429641 | Msa0970780:CDS | 40.0% | |
| !! | ATTTTCTTCTTCCTTGGTGC+CGG | - | chr6_4:2429774-2429793 | None:intergenic | 40.0% |
| ! | ATCCTAGCTTCTTTTGCCGT+TGG | - | chr6_4:2429729-2429748 | None:intergenic | 45.0% |
| GGCAAAAGAAGCTAGGATAC+CGG | + | chr6_4:2429731-2429750 | Msa0970780:CDS | 45.0% | |
| CACCAACGGCAAAAGAAGCT+AGG | + | chr6_4:2429724-2429743 | Msa0970780:CDS | 50.0% | |
| GGTGGACACGACATGTTCAC+CGG | - | chr6_4:2429753-2429772 | None:intergenic | 55.0% | |
| GGTGAACATGTCGTGTCCAC+CGG | + | chr6_4:2429752-2429771 | Msa0970780:CDS | 55.0% | |
| !! | TTCTTCTTCCTTGGTGCCGG+TGG | - | chr6_4:2429771-2429790 | None:intergenic | 55.0% |
| TGTCGTGTCCACCGGCACCA+AGG | + | chr6_4:2429760-2429779 | Msa0970780:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 2429552 | 2429887 | 2429552 | ID=Msa0970780;Name=Msa0970780 |
| chr6_4 | mRNA | 2429552 | 2429887 | 2429552 | ID=Msa0970780-mRNA-1;Parent=Msa0970780;Name=Msa0970780-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|111 |
| chr6_4 | exon | 2429552 | 2429887 | 2429552 | ID=Msa0970780-mRNA-1:exon:1145;Parent=Msa0970780-mRNA-1 |
| chr6_4 | CDS | 2429552 | 2429887 | 2429552 | ID=Msa0970780-mRNA-1:cds;Parent=Msa0970780-mRNA-1 |
| Gene Sequence |
| Protein sequence |
