AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0972570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0972570 MtrunA17_Chr6g0451441 53.571 140 49 2 1 124 319 458 1.78e-43 147
Msa0972570 MtrunA17_Chr6g0451421 51.402 107 52 0 11 117 23 129 2.57e-34 116
Msa0972570 MtrunA17_Chr2g0325301 44.037 109 61 0 12 120 26 134 1.85e-31 108
Msa0972570 MtrunA17_Chr6g0451411 44.915 118 59 1 1 112 175 292 2.19e-29 108
Msa0972570 MtrunA17_Chr7g0264421 40.566 106 63 0 12 117 220 325 2.89e-21 86.7
Msa0972570 MtrunA17_Chr2g0329701 40.566 106 63 0 12 117 250 355 5.64e-21 86.3
Msa0972570 MtrunA17_Chr6g0451451 50.000 68 34 0 50 117 1 68 1.59e-18 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0972570 AT3G01410.2 46.154 117 57 2 2 112 154 270 1.97e-24 95.1
Msa0972570 AT3G01410.1 46.154 117 57 2 2 112 154 270 1.97e-24 95.1
Msa0972570 AT1G24090.1 43.564 101 57 0 12 112 232 332 8.16e-19 80.5
Msa0972570 AT5G51080.3 39.604 101 61 0 12 112 138 238 1.37e-17 76.3
Msa0972570 AT5G51080.1 39.604 101 61 0 12 112 201 301 2.58e-17 76.3
Msa0972570 AT5G51080.2 39.604 101 61 0 12 112 201 301 2.58e-17 76.3

Find 44 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAGCCCCTAAGCTAATTT+TGG 0.153721 6_4:-4859858 None:intergenic
CTTTCTTGTACCGTTGTATT+TGG 0.214150 6_4:+4857867 Msa0972570:CDS
CTTTGCAGTACTATGGATTC+TGG 0.252287 6_4:+4858020 Msa0972570:intron
TATGGATTCTGGCAAGGATT+TGG 0.278405 6_4:+4858031 Msa0972570:CDS
CTCTGAGTTTGTTGTCAATC+AGG 0.282564 6_4:+4858150 Msa0972570:CDS
TATGTTTCTTTGCAGTACTA+TGG 0.304270 6_4:+4858013 Msa0972570:intron
ATGGATTCTGGCAAGGATTT+GGG 0.335466 6_4:+4858032 Msa0972570:CDS
TTTGAACTTGCCACGACATA+TGG 0.342170 6_4:+4859831 Msa0972570:three_prime_UTR
GTGTGTGTCGTTGGTCTGCC+AGG 0.395874 6_4:-4858494 None:intergenic
GCTTTACTGCTTGCTGATGA+TGG 0.411891 6_4:+4857912 Msa0972570:CDS
TGGATGCCCAAAATTAGCTT+AGG 0.436390 6_4:+4859851 Msa0972570:three_prime_UTR
CTTTACTGCTTGCTGATGAT+GGG 0.465599 6_4:+4857913 Msa0972570:CDS
ATGATTTATATTCCAAACTT+CGG 0.468125 6_4:-4859793 None:intergenic
AAACTCAGAGTCTCCTTTGA+CGG 0.469224 6_4:-4858137 None:intergenic
GCTTGCTGATGATGGGAGTC+AGG 0.473864 6_4:+4857920 Msa0972570:CDS
GGATGCCCAAAATTAGCTTA+GGG 0.478567 6_4:+4859852 Msa0972570:three_prime_UTR
ACACTTAAGGAGCTTATGTC+TGG 0.479927 6_4:+4858513 Msa0972570:CDS
AGACCAACGACACACACTTA+AGG 0.491634 6_4:+4858500 Msa0972570:CDS
TCTCCATCCAGCACATTCCT+CGG 0.496932 6_4:+4858569 Msa0972570:CDS
TTTAAACACGTTACCGTCAA+AGG 0.509214 6_4:+4858124 Msa0972570:CDS
CAGTACTATGGATTCTGGCA+AGG 0.514595 6_4:+4858025 Msa0972570:intron
ATGTCGTGGCAAGTTCAAAT+CGG 0.520480 6_4:-4859827 None:intergenic
CTCCATCCAGCACATTCCTC+GGG 0.523432 6_4:+4858570 Msa0972570:CDS
CAATTACCCGAGGAATGTGC+TGG 0.523567 6_4:-4858576 None:intergenic
AGTAAAGCTCCTGCACCAGC+TGG 0.540297 6_4:-4857897 None:intergenic
TCCAACAGTTTGGGGAACCC+TGG 0.542372 6_4:+4858476 Msa0972570:intron
ATGAGACAGGCGATCATGAA+AGG 0.548157 6_4:+4858100 Msa0972570:CDS
GGTGCTGAACCAGCTGGTGC+AGG 0.551244 6_4:+4857888 Msa0972570:CDS
GTATTTGGTGCTGAACCAGC+TGG 0.553076 6_4:+4857882 Msa0972570:CDS
GTCTGGAGGCTATGAAGTTG+AGG 0.555604 6_4:+4858530 Msa0972570:CDS
GCTCCTTAAGTGTGTGTCGT+TGG 0.557850 6_4:-4858503 None:intergenic
CCACCACATTATATCACGAC+TGG 0.577743 6_4:+4859937 Msa0972570:three_prime_UTR
TACCCGAGGAATGTGCTGGA+TGG 0.580365 6_4:-4858572 None:intergenic
GATGCCCAAAATTAGCTTAG+GGG 0.582782 6_4:+4859853 Msa0972570:three_prime_UTR
TGTGTGTCGTTGGTCTGCCA+GGG 0.596653 6_4:-4858493 None:intergenic
ATTTGGGCATCAAACAAAAG+AGG 0.600961 6_4:+4858048 Msa0972570:CDS
TTTGGGCATCAAACAAAAGA+GGG 0.614208 6_4:+4858049 Msa0972570:CDS
TCGCCAGTCGTGATATAATG+TGG 0.632791 6_4:-4859940 None:intergenic
CTTAAGGAGCTTATGTCTGG+AGG 0.659047 6_4:+4858516 Msa0972570:CDS
CCAGTCGTGATATAATGTGG+TGG 0.682461 6_4:-4859937 None:intergenic
TGGGCATCAAACAAAAGAGG+GGG 0.687228 6_4:+4858051 Msa0972570:CDS
ACAATAACATCAAACATCTG+CGG 0.712173 6_4:-4859963 None:intergenic
TTGGGCATCAAACAAAAGAG+GGG 0.719635 6_4:+4858050 Msa0972570:CDS
TGGTTCAGCACCAAATACAA+CGG 0.727062 6_4:-4857877 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTAAATATTCATATAATT+AGG - chr6_4:4859191-4859210 None:intergenic 10.0%
!! ATGTAAATATTCATATAATT+AGG - chr6_4:4859191-4859210 None:intergenic 10.0%
!!! TTTTTTTTATCTCTTCAATT+TGG + chr6_4:4859222-4859241 Msa0972570:intron 15.0%
!! ATTAGAAAAAGAAATGTTAT+TGG - chr6_4:4859282-4859301 None:intergenic 15.0%
!!! ATTTCTTTTTCTAATGTTTA+AGG + chr6_4:4859287-4859306 Msa0972570:intron 15.0%
!!! TTTCTTTTTCTAATGTTTAA+GGG + chr6_4:4859288-4859307 Msa0972570:intron 15.0%
!!! TTTTTTTTATCTCTTCAATT+TGG + chr6_4:4859222-4859241 Msa0972570:intron 15.0%
!! ATTAGAAAAAGAAATGTTAT+TGG - chr6_4:4859282-4859301 None:intergenic 15.0%
!!! ATTTCTTTTTCTAATGTTTA+AGG + chr6_4:4859287-4859306 Msa0972570:intron 15.0%
!!! TTTCTTTTTCTAATGTTTAA+GGG + chr6_4:4859288-4859307 Msa0972570:intron 15.0%
!! TTCTTTACTTCATCTTAAAA+TGG + chr6_4:4858216-4858235 Msa0972570:intron 20.0%
!!! GTAGAAAGTATTTAATTACT+TGG - chr6_4:4858288-4858307 None:intergenic 20.0%
!!! CTTTGTTATTTTTATAAAGG+GGG - chr6_4:4858338-4858357 None:intergenic 20.0%
!!! CCTTTGTTATTTTTATAAAG+GGG - chr6_4:4858339-4858358 None:intergenic 20.0%
!!! GCCTTTGTTATTTTTATAAA+GGG - chr6_4:4858340-4858359 None:intergenic 20.0%
!!! AGCCTTTGTTATTTTTATAA+AGG - chr6_4:4858341-4858360 None:intergenic 20.0%
!! AAACAATCTTTACATGAATT+TGG - chr6_4:4858729-4858748 None:intergenic 20.0%
!! AAACAATCTTTCCATAAATT+TGG + chr6_4:4858891-4858910 Msa0972570:intron 20.0%
!! TATAGGAATTCTCATTTATT+AGG - chr6_4:4859417-4859436 None:intergenic 20.0%
!! TTTAAACCAACAAGTATATT+TGG + chr6_4:4859456-4859475 Msa0972570:intron 20.0%
!! ATTGAAAGTAATATTGTGAA+AGG + chr6_4:4859523-4859542 Msa0972570:intron 20.0%
!!! GGATATGAAAATAAATTTTG+AGG - chr6_4:4859598-4859617 None:intergenic 20.0%
!! TTATAATCCTGTATCAAATA+GGG - chr6_4:4859619-4859638 None:intergenic 20.0%
!! TTTATAATCCTGTATCAAAT+AGG - chr6_4:4859620-4859639 None:intergenic 20.0%
!! TACAGGATTATAAAATCTAA+TGG + chr6_4:4859626-4859645 Msa0972570:intron 20.0%
!!! TTTTGTTATACTTTTGTAGA+TGG + chr6_4:4859653-4859672 Msa0972570:intron 20.0%
!!! CATTTCTTGTTTTAGTATAT+TGG + chr6_4:4859753-4859772 Msa0972570:three_prime_UTR 20.0%
!! ATGATTTATATTCCAAACTT+CGG - chr6_4:4859796-4859815 None:intergenic 20.0%
!! TTCTTTACTTCATCTTAAAA+TGG + chr6_4:4858216-4858235 Msa0972570:intron 20.0%
!!! GTAGAAAGTATTTAATTACT+TGG - chr6_4:4858288-4858307 None:intergenic 20.0%
!!! CTTTGTTATTTTTATAAAGG+GGG - chr6_4:4858338-4858357 None:intergenic 20.0%
!!! CCTTTGTTATTTTTATAAAG+GGG - chr6_4:4858339-4858358 None:intergenic 20.0%
!!! GCCTTTGTTATTTTTATAAA+GGG - chr6_4:4858340-4858359 None:intergenic 20.0%
!!! AGCCTTTGTTATTTTTATAA+AGG - chr6_4:4858341-4858360 None:intergenic 20.0%
!! AAACAATCTTTACATGAATT+TGG - chr6_4:4858729-4858748 None:intergenic 20.0%
!! AAACAATCTTTCCATAAATT+TGG + chr6_4:4858891-4858910 Msa0972570:intron 20.0%
!! TATAGGAATTCTCATTTATT+AGG - chr6_4:4859417-4859436 None:intergenic 20.0%
!! TTTAAACCAACAAGTATATT+TGG + chr6_4:4859456-4859475 Msa0972570:intron 20.0%
!! ATTGAAAGTAATATTGTGAA+AGG + chr6_4:4859523-4859542 Msa0972570:intron 20.0%
!!! GGATATGAAAATAAATTTTG+AGG - chr6_4:4859598-4859617 None:intergenic 20.0%
!! TTATAATCCTGTATCAAATA+GGG - chr6_4:4859619-4859638 None:intergenic 20.0%
!! TTTATAATCCTGTATCAAAT+AGG - chr6_4:4859620-4859639 None:intergenic 20.0%
!! TACAGGATTATAAAATCTAA+TGG + chr6_4:4859626-4859645 Msa0972570:intron 20.0%
!!! TTTTGTTATACTTTTGTAGA+TGG + chr6_4:4859653-4859672 Msa0972570:intron 20.0%
!!! CATTTCTTGTTTTAGTATAT+TGG + chr6_4:4859753-4859772 Msa0972570:three_prime_UTR 20.0%
!! ATGATTTATATTCCAAACTT+CGG - chr6_4:4859796-4859815 None:intergenic 20.0%
! CCCCTTTATAAAAATAACAA+AGG + chr6_4:4858336-4858355 Msa0972570:intron 25.0%
! TTAACAGTATCTAACCATTT+AGG - chr6_4:4858380-4858399 None:intergenic 25.0%
! AATTTACTGACACATGTTAT+TGG + chr6_4:4858450-4858469 Msa0972570:intron 25.0%
! GAGTTCAAAAACAATTACAT+TGG + chr6_4:4858606-4858625 Msa0972570:intron 25.0%
!! TTTTAAATGCAACCATTTAC+TGG + chr6_4:4858658-4858677 Msa0972570:intron 25.0%
! ATACATGTCTACCAAATTTA+TGG - chr6_4:4858905-4858924 None:intergenic 25.0%
! TTTGTTAGGATTCTTATGAT+AGG + chr6_4:4859041-4859060 Msa0972570:intron 25.0%
!! AACAATATATCTAAGATGCT+GGG - chr6_4:4859138-4859157 None:intergenic 25.0%
! AAAAGAAATGTTATTGGTAG+AGG - chr6_4:4859276-4859295 None:intergenic 25.0%
! GTAATATTGTGAAAGGTTAA+TGG + chr6_4:4859530-4859549 Msa0972570:intron 25.0%
!!! TTCTTGTTTTAGTATATTGG+AGG + chr6_4:4859756-4859775 Msa0972570:three_prime_UTR 25.0%
! ATCTTAAAAACTCTTCTATG+TGG + chr6_4:4859911-4859930 Msa0972570:three_prime_UTR 25.0%
! CCCCTTTATAAAAATAACAA+AGG + chr6_4:4858336-4858355 Msa0972570:intron 25.0%
! TTAACAGTATCTAACCATTT+AGG - chr6_4:4858380-4858399 None:intergenic 25.0%
! AATTTACTGACACATGTTAT+TGG + chr6_4:4858450-4858469 Msa0972570:intron 25.0%
! GAGTTCAAAAACAATTACAT+TGG + chr6_4:4858606-4858625 Msa0972570:intron 25.0%
!! TTTTAAATGCAACCATTTAC+TGG + chr6_4:4858658-4858677 Msa0972570:intron 25.0%
! ATACATGTCTACCAAATTTA+TGG - chr6_4:4858905-4858924 None:intergenic 25.0%
! TTTGTTAGGATTCTTATGAT+AGG + chr6_4:4859041-4859060 Msa0972570:intron 25.0%
!! AACAATATATCTAAGATGCT+GGG - chr6_4:4859138-4859157 None:intergenic 25.0%
! AAAAGAAATGTTATTGGTAG+AGG - chr6_4:4859276-4859295 None:intergenic 25.0%
! GTAATATTGTGAAAGGTTAA+TGG + chr6_4:4859530-4859549 Msa0972570:intron 25.0%
!!! TTCTTGTTTTAGTATATTGG+AGG + chr6_4:4859756-4859775 Msa0972570:three_prime_UTR 25.0%
! ATCTTAAAAACTCTTCTATG+TGG + chr6_4:4859911-4859930 Msa0972570:three_prime_UTR 25.0%
ATTCTATGCAACAGTTCATT+TGG + chr6_4:4857948-4857967 Msa0972570:intron 30.0%
TTCTATGCAACAGTTCATTT+GGG + chr6_4:4857949-4857968 Msa0972570:intron 30.0%
TATGTTTCTTTGCAGTACTA+TGG + chr6_4:4858013-4858032 Msa0972570:intron 30.0%
ACATTAATAGTAGCAACCTA+AGG + chr6_4:4858306-4858325 Msa0972570:intron 30.0%
!!! GTTATTTTTATAAAGGGGGT+AGG - chr6_4:4858334-4858353 None:intergenic 30.0%
TTCAATCCATTGTTCCTAAA+TGG + chr6_4:4858363-4858382 Msa0972570:intron 30.0%
ATCTAACCATTTAGGAACAA+TGG - chr6_4:4858372-4858391 None:intergenic 30.0%
CATGTGTCAGTAAATTGTTT+AGG - chr6_4:4858446-4858465 None:intergenic 30.0%
!!! TTATTGGTTTTCCAACAGTT+TGG + chr6_4:4858466-4858485 Msa0972570:intron 30.0%
!!! TATTGGTTTTCCAACAGTTT+GGG + chr6_4:4858467-4858486 Msa0972570:intron 30.0%
CTTAACAAGATACCTATTGA+AGG - chr6_4:4858765-4858784 None:intergenic 30.0%
CTTCAATAGGTATCTTGTTA+AGG + chr6_4:4858763-4858782 Msa0972570:intron 30.0%
TAGGTATCTTGTTAAGGAAA+TGG + chr6_4:4858769-4858788 Msa0972570:intron 30.0%
!! GTAGAAAGTATTGCTAGATT+AGG + chr6_4:4858798-4858817 Msa0972570:intron 30.0%
AGTAAGTGTATAGCAAACTA+AGG - chr6_4:4858836-4858855 None:intergenic 30.0%
CTCAAGATATTCAGAAACTT+AGG - chr6_4:4859109-4859128 None:intergenic 30.0%
! GAACAATATATCTAAGATGC+TGG - chr6_4:4859139-4859158 None:intergenic 30.0%
AATTAGGATCCTAATGCAAT+TGG - chr6_4:4859175-4859194 None:intergenic 30.0%
! ACATTTTCTCAGTTCTCTAT+AGG + chr6_4:4859374-4859393 Msa0972570:intron 30.0%
ACACAACCAAATATACTTGT+TGG - chr6_4:4859465-4859484 None:intergenic 30.0%
CAAGTATATTTGGTTGTGTT+TGG + chr6_4:4859466-4859485 Msa0972570:intron 30.0%
!! TTGTGAAAGGTTAATGGATT+TGG + chr6_4:4859536-4859555 Msa0972570:intron 30.0%
!! AAAGGTTAATGGATTTGGTA+GGG + chr6_4:4859541-4859560 Msa0972570:intron 30.0%
TTCATATCCCTATTTGATAC+AGG + chr6_4:4859609-4859628 Msa0972570:intron 30.0%
!!! TTGTTTTAGTATATTGGAGG+AGG + chr6_4:4859759-4859778 Msa0972570:three_prime_UTR 30.0%
ATTCTATGCAACAGTTCATT+TGG + chr6_4:4857948-4857967 Msa0972570:intron 30.0%
TTCTATGCAACAGTTCATTT+GGG + chr6_4:4857949-4857968 Msa0972570:intron 30.0%
TATGTTTCTTTGCAGTACTA+TGG + chr6_4:4858013-4858032 Msa0972570:intron 30.0%
ACATTAATAGTAGCAACCTA+AGG + chr6_4:4858306-4858325 Msa0972570:intron 30.0%
!!! GTTATTTTTATAAAGGGGGT+AGG - chr6_4:4858334-4858353 None:intergenic 30.0%
TTCAATCCATTGTTCCTAAA+TGG + chr6_4:4858363-4858382 Msa0972570:intron 30.0%
ATCTAACCATTTAGGAACAA+TGG - chr6_4:4858372-4858391 None:intergenic 30.0%
CATGTGTCAGTAAATTGTTT+AGG - chr6_4:4858446-4858465 None:intergenic 30.0%
!!! TTATTGGTTTTCCAACAGTT+TGG + chr6_4:4858466-4858485 Msa0972570:intron 30.0%
!!! TATTGGTTTTCCAACAGTTT+GGG + chr6_4:4858467-4858486 Msa0972570:intron 30.0%
CTTAACAAGATACCTATTGA+AGG - chr6_4:4858765-4858784 None:intergenic 30.0%
CTTCAATAGGTATCTTGTTA+AGG + chr6_4:4858763-4858782 Msa0972570:intron 30.0%
TAGGTATCTTGTTAAGGAAA+TGG + chr6_4:4858769-4858788 Msa0972570:intron 30.0%
!! GTAGAAAGTATTGCTAGATT+AGG + chr6_4:4858798-4858817 Msa0972570:intron 30.0%
AGTAAGTGTATAGCAAACTA+AGG - chr6_4:4858836-4858855 None:intergenic 30.0%
CTCAAGATATTCAGAAACTT+AGG - chr6_4:4859109-4859128 None:intergenic 30.0%
! GAACAATATATCTAAGATGC+TGG - chr6_4:4859139-4859158 None:intergenic 30.0%
AATTAGGATCCTAATGCAAT+TGG - chr6_4:4859175-4859194 None:intergenic 30.0%
! ACATTTTCTCAGTTCTCTAT+AGG + chr6_4:4859374-4859393 Msa0972570:intron 30.0%
ACACAACCAAATATACTTGT+TGG - chr6_4:4859465-4859484 None:intergenic 30.0%
CAAGTATATTTGGTTGTGTT+TGG + chr6_4:4859466-4859485 Msa0972570:intron 30.0%
!! TTGTGAAAGGTTAATGGATT+TGG + chr6_4:4859536-4859555 Msa0972570:intron 30.0%
!! AAAGGTTAATGGATTTGGTA+GGG + chr6_4:4859541-4859560 Msa0972570:intron 30.0%
TTCATATCCCTATTTGATAC+AGG + chr6_4:4859609-4859628 Msa0972570:intron 30.0%
!!! TTGTTTTAGTATATTGGAGG+AGG + chr6_4:4859759-4859778 Msa0972570:three_prime_UTR 30.0%
CTTTCTTGTACCGTTGTATT+TGG + chr6_4:4857867-4857886 Msa0972570:CDS 35.0%
TCTATGCAACAGTTCATTTG+GGG + chr6_4:4857950-4857969 Msa0972570:intron 35.0%
CTGCAAAGAAACATAAGAGT+TGG - chr6_4:4858008-4858027 None:intergenic 35.0%
ATTTGGGCATCAAACAAAAG+AGG + chr6_4:4858048-4858067 Msa0972570:CDS 35.0%
TTTGGGCATCAAACAAAAGA+GGG + chr6_4:4858049-4858068 Msa0972570:CDS 35.0%
!! GTTGATTTTAGGTATGAGAC+AGG + chr6_4:4858087-4858106 Msa0972570:CDS 35.0%
TTTAAACACGTTACCGTCAA+AGG + chr6_4:4858124-4858143 Msa0972570:CDS 35.0%
!!! ATTGGTTTTCCAACAGTTTG+GGG + chr6_4:4858468-4858487 Msa0972570:intron 35.0%
CCATGAATAGAACCAGTAAA+TGG - chr6_4:4858673-4858692 None:intergenic 35.0%
!! CCATTTACTGGTTCTATTCA+TGG + chr6_4:4858670-4858689 Msa0972570:intron 35.0%
! CATTTTCTGTCACCTTCAAT+AGG + chr6_4:4858750-4858769 Msa0972570:intron 35.0%
CAGTATATAACTAGCTAGGA+TGG - chr6_4:4858950-4858969 None:intergenic 35.0%
CGAACAGTATATAACTAGCT+AGG - chr6_4:4858954-4858973 None:intergenic 35.0%
GATTGTACCAAGCAAATACT+GGG - chr6_4:4859002-4859021 None:intergenic 35.0%
TGATTGTACCAAGCAAATAC+TGG - chr6_4:4859003-4859022 None:intergenic 35.0%
GCATTAGCAGTAACTTTGTT+AGG + chr6_4:4859027-4859046 Msa0972570:intron 35.0%
AATGTTATTGGTAGAGGTTG+AGG - chr6_4:4859270-4859289 None:intergenic 35.0%
GGCATAACCTTGGAAATTTA+AGG - chr6_4:4859349-4859368 None:intergenic 35.0%
AGAACTGAGAAAATGTCTTG+AGG - chr6_4:4859370-4859389 None:intergenic 35.0%
! CAGCTTTGGTTGCAAAATAT+AGG - chr6_4:4859434-4859453 None:intergenic 35.0%
!! TGTTGGTTTAAAAGCAGCTT+TGG - chr6_4:4859448-4859467 None:intergenic 35.0%
!! GAAAGGTTAATGGATTTGGT+AGG + chr6_4:4859540-4859559 Msa0972570:intron 35.0%
!! AAGGTTAATGGATTTGGTAG+GGG + chr6_4:4859542-4859561 Msa0972570:intron 35.0%
CATGACACTTGCAAAAACTA+TGG + chr6_4:4859718-4859737 Msa0972570:exon 35.0%
! GTTACAATTTTGCCGAAGTT+TGG + chr6_4:4859781-4859800 Msa0972570:three_prime_UTR 35.0%
! TAAGCCCCTAAGCTAATTTT+GGG - chr6_4:4859860-4859879 None:intergenic 35.0%
! TTAAGCCCCTAAGCTAATTT+TGG - chr6_4:4859861-4859880 None:intergenic 35.0%
CTTTCTTGTACCGTTGTATT+TGG + chr6_4:4857867-4857886 Msa0972570:CDS 35.0%
TCTATGCAACAGTTCATTTG+GGG + chr6_4:4857950-4857969 Msa0972570:intron 35.0%
CTGCAAAGAAACATAAGAGT+TGG - chr6_4:4858008-4858027 None:intergenic 35.0%
ATTTGGGCATCAAACAAAAG+AGG + chr6_4:4858048-4858067 Msa0972570:CDS 35.0%
TTTGGGCATCAAACAAAAGA+GGG + chr6_4:4858049-4858068 Msa0972570:CDS 35.0%
!! GTTGATTTTAGGTATGAGAC+AGG + chr6_4:4858087-4858106 Msa0972570:CDS 35.0%
TTTAAACACGTTACCGTCAA+AGG + chr6_4:4858124-4858143 Msa0972570:CDS 35.0%
!!! ATTGGTTTTCCAACAGTTTG+GGG + chr6_4:4858468-4858487 Msa0972570:intron 35.0%
CCATGAATAGAACCAGTAAA+TGG - chr6_4:4858673-4858692 None:intergenic 35.0%
!! CCATTTACTGGTTCTATTCA+TGG + chr6_4:4858670-4858689 Msa0972570:intron 35.0%
! CATTTTCTGTCACCTTCAAT+AGG + chr6_4:4858750-4858769 Msa0972570:intron 35.0%
CAGTATATAACTAGCTAGGA+TGG - chr6_4:4858950-4858969 None:intergenic 35.0%
CGAACAGTATATAACTAGCT+AGG - chr6_4:4858954-4858973 None:intergenic 35.0%
GATTGTACCAAGCAAATACT+GGG - chr6_4:4859002-4859021 None:intergenic 35.0%
TGATTGTACCAAGCAAATAC+TGG - chr6_4:4859003-4859022 None:intergenic 35.0%
GCATTAGCAGTAACTTTGTT+AGG + chr6_4:4859027-4859046 Msa0972570:intron 35.0%
AATGTTATTGGTAGAGGTTG+AGG - chr6_4:4859270-4859289 None:intergenic 35.0%
GGCATAACCTTGGAAATTTA+AGG - chr6_4:4859349-4859368 None:intergenic 35.0%
AGAACTGAGAAAATGTCTTG+AGG - chr6_4:4859370-4859389 None:intergenic 35.0%
! CAGCTTTGGTTGCAAAATAT+AGG - chr6_4:4859434-4859453 None:intergenic 35.0%
!! TGTTGGTTTAAAAGCAGCTT+TGG - chr6_4:4859448-4859467 None:intergenic 35.0%
!! GAAAGGTTAATGGATTTGGT+AGG + chr6_4:4859540-4859559 Msa0972570:intron 35.0%
!! AAGGTTAATGGATTTGGTAG+GGG + chr6_4:4859542-4859561 Msa0972570:intron 35.0%
CATGACACTTGCAAAAACTA+TGG + chr6_4:4859718-4859737 Msa0972570:exon 35.0%
! GTTACAATTTTGCCGAAGTT+TGG + chr6_4:4859781-4859800 Msa0972570:three_prime_UTR 35.0%
! TAAGCCCCTAAGCTAATTTT+GGG - chr6_4:4859860-4859879 None:intergenic 35.0%
! TTAAGCCCCTAAGCTAATTT+TGG - chr6_4:4859861-4859880 None:intergenic 35.0%
TGGTTCAGCACCAAATACAA+CGG - chr6_4:4857880-4857899 None:intergenic 40.0%
! CTTTACTGCTTGCTGATGAT+GGG + chr6_4:4857913-4857932 Msa0972570:CDS 40.0%
CTTTGCAGTACTATGGATTC+TGG + chr6_4:4858020-4858039 Msa0972570:intron 40.0%
TATGGATTCTGGCAAGGATT+TGG + chr6_4:4858031-4858050 Msa0972570:CDS 40.0%
ATGGATTCTGGCAAGGATTT+GGG + chr6_4:4858032-4858051 Msa0972570:CDS 40.0%
TTGGGCATCAAACAAAAGAG+GGG + chr6_4:4858050-4858069 Msa0972570:CDS 40.0%
AAACTCAGAGTCTCCTTTGA+CGG - chr6_4:4858140-4858159 None:intergenic 40.0%
!! CTCTGAGTTTGTTGTCAATC+AGG + chr6_4:4858150-4858169 Msa0972570:CDS 40.0%
ACACTTAAGGAGCTTATGTC+TGG + chr6_4:4858513-4858532 Msa0972570:CDS 40.0%
CACAATTCCCAGTATTTGCT+TGG + chr6_4:4858992-4859011 Msa0972570:intron 40.0%
TCACTGACACCAATTGCATT+AGG + chr6_4:4859163-4859182 Msa0972570:intron 40.0%
! AACCTTGGAAATTTAAGGCC+CGG - chr6_4:4859344-4859363 None:intergenic 40.0%
AATGTCTTGAGGCATAACCT+TGG - chr6_4:4859359-4859378 None:intergenic 40.0%
ATGTCGTGGCAAGTTCAAAT+CGG - chr6_4:4859830-4859849 None:intergenic 40.0%
TTTGAACTTGCCACGACATA+TGG + chr6_4:4859831-4859850 Msa0972570:three_prime_UTR 40.0%
! TGGATGCCCAAAATTAGCTT+AGG + chr6_4:4859851-4859870 Msa0972570:three_prime_UTR 40.0%
! GGATGCCCAAAATTAGCTTA+GGG + chr6_4:4859852-4859871 Msa0972570:three_prime_UTR 40.0%
! GATGCCCAAAATTAGCTTAG+GGG + chr6_4:4859853-4859872 Msa0972570:three_prime_UTR 40.0%
TGGTTCAGCACCAAATACAA+CGG - chr6_4:4857880-4857899 None:intergenic 40.0%
! CTTTACTGCTTGCTGATGAT+GGG + chr6_4:4857913-4857932 Msa0972570:CDS 40.0%
CTTTGCAGTACTATGGATTC+TGG + chr6_4:4858020-4858039 Msa0972570:intron 40.0%
TATGGATTCTGGCAAGGATT+TGG + chr6_4:4858031-4858050 Msa0972570:CDS 40.0%
ATGGATTCTGGCAAGGATTT+GGG + chr6_4:4858032-4858051 Msa0972570:CDS 40.0%
TTGGGCATCAAACAAAAGAG+GGG + chr6_4:4858050-4858069 Msa0972570:CDS 40.0%
AAACTCAGAGTCTCCTTTGA+CGG - chr6_4:4858140-4858159 None:intergenic 40.0%
!! CTCTGAGTTTGTTGTCAATC+AGG + chr6_4:4858150-4858169 Msa0972570:CDS 40.0%
ACACTTAAGGAGCTTATGTC+TGG + chr6_4:4858513-4858532 Msa0972570:CDS 40.0%
CACAATTCCCAGTATTTGCT+TGG + chr6_4:4858992-4859011 Msa0972570:intron 40.0%
TCACTGACACCAATTGCATT+AGG + chr6_4:4859163-4859182 Msa0972570:intron 40.0%
! AACCTTGGAAATTTAAGGCC+CGG - chr6_4:4859344-4859363 None:intergenic 40.0%
AATGTCTTGAGGCATAACCT+TGG - chr6_4:4859359-4859378 None:intergenic 40.0%
ATGTCGTGGCAAGTTCAAAT+CGG - chr6_4:4859830-4859849 None:intergenic 40.0%
TTTGAACTTGCCACGACATA+TGG + chr6_4:4859831-4859850 Msa0972570:three_prime_UTR 40.0%
! TGGATGCCCAAAATTAGCTT+AGG + chr6_4:4859851-4859870 Msa0972570:three_prime_UTR 40.0%
! GGATGCCCAAAATTAGCTTA+GGG + chr6_4:4859852-4859871 Msa0972570:three_prime_UTR 40.0%
! GATGCCCAAAATTAGCTTAG+GGG + chr6_4:4859853-4859872 Msa0972570:three_prime_UTR 40.0%
! GCTTTACTGCTTGCTGATGA+TGG + chr6_4:4857912-4857931 Msa0972570:CDS 45.0%
CAGTACTATGGATTCTGGCA+AGG + chr6_4:4858025-4858044 Msa0972570:intron 45.0%
TGGGCATCAAACAAAAGAGG+GGG + chr6_4:4858051-4858070 Msa0972570:CDS 45.0%
!!! CAGTATCGGGCGTTGATTTT+AGG + chr6_4:4858076-4858095 Msa0972570:CDS 45.0%
ATGAGACAGGCGATCATGAA+AGG + chr6_4:4858100-4858119 Msa0972570:CDS 45.0%
ATAAAGGGGGTAGGTTCCTT+AGG - chr6_4:4858325-4858344 None:intergenic 45.0%
AGACCAACGACACACACTTA+AGG + chr6_4:4858500-4858519 Msa0972570:CDS 45.0%
CTTAAGGAGCTTATGTCTGG+AGG + chr6_4:4858516-4858535 Msa0972570:CDS 45.0%
CTCTTCAAAGCAATTACCCG+AGG - chr6_4:4858589-4858608 None:intergenic 45.0%
CAGCTCGTTATTATGCAAGC+CGG + chr6_4:4859322-4859341 Msa0972570:intron 45.0%
AGCTCGTTATTATGCAAGCC+GGG + chr6_4:4859323-4859342 Msa0972570:intron 45.0%
! TTTTGGGCATCCATATGTCG+TGG - chr6_4:4859844-4859863 None:intergenic 45.0%
CCAGTCGTGATATAATGTGG+TGG - chr6_4:4859940-4859959 None:intergenic 45.0%
CCACCACATTATATCACGAC+TGG + chr6_4:4859937-4859956 Msa0972570:three_prime_UTR 45.0%
TCGCCAGTCGTGATATAATG+TGG - chr6_4:4859943-4859962 None:intergenic 45.0%
! GCTTTACTGCTTGCTGATGA+TGG + chr6_4:4857912-4857931 Msa0972570:CDS 45.0%
CAGTACTATGGATTCTGGCA+AGG + chr6_4:4858025-4858044 Msa0972570:intron 45.0%
TGGGCATCAAACAAAAGAGG+GGG + chr6_4:4858051-4858070 Msa0972570:CDS 45.0%
!!! CAGTATCGGGCGTTGATTTT+AGG + chr6_4:4858076-4858095 Msa0972570:CDS 45.0%
ATGAGACAGGCGATCATGAA+AGG + chr6_4:4858100-4858119 Msa0972570:CDS 45.0%
ATAAAGGGGGTAGGTTCCTT+AGG - chr6_4:4858325-4858344 None:intergenic 45.0%
AGACCAACGACACACACTTA+AGG + chr6_4:4858500-4858519 Msa0972570:CDS 45.0%
CTTAAGGAGCTTATGTCTGG+AGG + chr6_4:4858516-4858535 Msa0972570:CDS 45.0%
CTCTTCAAAGCAATTACCCG+AGG - chr6_4:4858589-4858608 None:intergenic 45.0%
CAGCTCGTTATTATGCAAGC+CGG + chr6_4:4859322-4859341 Msa0972570:intron 45.0%
AGCTCGTTATTATGCAAGCC+GGG + chr6_4:4859323-4859342 Msa0972570:intron 45.0%
! TTTTGGGCATCCATATGTCG+TGG - chr6_4:4859844-4859863 None:intergenic 45.0%
CCAGTCGTGATATAATGTGG+TGG - chr6_4:4859940-4859959 None:intergenic 45.0%
CCACCACATTATATCACGAC+TGG + chr6_4:4859937-4859956 Msa0972570:three_prime_UTR 45.0%
TCGCCAGTCGTGATATAATG+TGG - chr6_4:4859943-4859962 None:intergenic 45.0%
!! GTATTTGGTGCTGAACCAGC+TGG + chr6_4:4857882-4857901 Msa0972570:CDS 50.0%
GCTCCTTAAGTGTGTGTCGT+TGG - chr6_4:4858506-4858525 None:intergenic 50.0%
GTCTGGAGGCTATGAAGTTG+AGG + chr6_4:4858530-4858549 Msa0972570:CDS 50.0%
TCTCCATCCAGCACATTCCT+CGG + chr6_4:4858569-4858588 Msa0972570:CDS 50.0%
CAATTACCCGAGGAATGTGC+TGG - chr6_4:4858579-4858598 None:intergenic 50.0%
AGCCGGGCCTTAAATTTCCA+AGG + chr6_4:4859339-4859358 Msa0972570:intron 50.0%
!! GTATTTGGTGCTGAACCAGC+TGG + chr6_4:4857882-4857901 Msa0972570:CDS 50.0%
GCTCCTTAAGTGTGTGTCGT+TGG - chr6_4:4858506-4858525 None:intergenic 50.0%
GTCTGGAGGCTATGAAGTTG+AGG + chr6_4:4858530-4858549 Msa0972570:CDS 50.0%
TCTCCATCCAGCACATTCCT+CGG + chr6_4:4858569-4858588 Msa0972570:CDS 50.0%
CAATTACCCGAGGAATGTGC+TGG - chr6_4:4858579-4858598 None:intergenic 50.0%
AGCCGGGCCTTAAATTTCCA+AGG + chr6_4:4859339-4859358 Msa0972570:intron 50.0%
AGTAAAGCTCCTGCACCAGC+TGG - chr6_4:4857900-4857919 None:intergenic 55.0%
!! GCTTGCTGATGATGGGAGTC+AGG + chr6_4:4857920-4857939 Msa0972570:CDS 55.0%
! CAAAAGAGGGGGCGCAGTAT+CGG + chr6_4:4858062-4858081 Msa0972570:CDS 55.0%
! AAAAGAGGGGGCGCAGTATC+GGG + chr6_4:4858063-4858082 Msa0972570:CDS 55.0%
TCCAACAGTTTGGGGAACCC+TGG + chr6_4:4858476-4858495 Msa0972570:intron 55.0%
! TGTGTGTCGTTGGTCTGCCA+GGG - chr6_4:4858496-4858515 None:intergenic 55.0%
CTCCATCCAGCACATTCCTC+GGG + chr6_4:4858570-4858589 Msa0972570:CDS 55.0%
TACCCGAGGAATGTGCTGGA+TGG - chr6_4:4858575-4858594 None:intergenic 55.0%
AGTAAAGCTCCTGCACCAGC+TGG - chr6_4:4857900-4857919 None:intergenic 55.0%
!! GCTTGCTGATGATGGGAGTC+AGG + chr6_4:4857920-4857939 Msa0972570:CDS 55.0%
! CAAAAGAGGGGGCGCAGTAT+CGG + chr6_4:4858062-4858081 Msa0972570:CDS 55.0%
! AAAAGAGGGGGCGCAGTATC+GGG + chr6_4:4858063-4858082 Msa0972570:CDS 55.0%
TCCAACAGTTTGGGGAACCC+TGG + chr6_4:4858476-4858495 Msa0972570:intron 55.0%
! TGTGTGTCGTTGGTCTGCCA+GGG - chr6_4:4858496-4858515 None:intergenic 55.0%
CTCCATCCAGCACATTCCTC+GGG + chr6_4:4858570-4858589 Msa0972570:CDS 55.0%
TACCCGAGGAATGTGCTGGA+TGG - chr6_4:4858575-4858594 None:intergenic 55.0%
GCCAGGGTTCCCCAAACTGT+TGG - chr6_4:4858480-4858499 None:intergenic 60.0%
! GTGTGTGTCGTTGGTCTGCC+AGG - chr6_4:4858497-4858516 None:intergenic 60.0%
GCCAGGGTTCCCCAAACTGT+TGG - chr6_4:4858480-4858499 None:intergenic 60.0%
! GTGTGTGTCGTTGGTCTGCC+AGG - chr6_4:4858497-4858516 None:intergenic 60.0%
! GGTGCTGAACCAGCTGGTGC+AGG + chr6_4:4857888-4857907 Msa0972570:CDS 65.0%
! GGTGCTGAACCAGCTGGTGC+AGG + chr6_4:4857888-4857907 Msa0972570:CDS 65.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 4857867 4859975 4857867 ID=Msa0972570;Name=Msa0972570
chr6_4 mRNA 4857867 4859975 4857867 ID=Msa0972570-mRNA-1;Parent=Msa0972570;Name=Msa0972570-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0.66|0.75|1|1|1|4|253|125
chr6_4 exon 4857867 4857941 4857867 ID=Msa0972570-mRNA-1:exon:2055;Parent=Msa0972570-mRNA-1
chr6_4 exon 4858028 4858171 4858028 ID=Msa0972570-mRNA-1:exon:2056;Parent=Msa0972570-mRNA-1
chr6_4 exon 4858484 4858591 4858484 ID=Msa0972570-mRNA-1:exon:2057;Parent=Msa0972570-mRNA-1
chr6_4 exon 4859672 4859975 4859672 ID=Msa0972570-mRNA-1:exon:2058;Parent=Msa0972570-mRNA-1
chr6_4 CDS 4857867 4857941 4857867 ID=Msa0972570-mRNA-1:cds;Parent=Msa0972570-mRNA-1
chr6_4 CDS 4858028 4858171 4858028 ID=Msa0972570-mRNA-1:cds;Parent=Msa0972570-mRNA-1
chr6_4 CDS 4858484 4858591 4858484 ID=Msa0972570-mRNA-1:cds;Parent=Msa0972570-mRNA-1
chr6_4 CDS 4859672 4859722 4859672 ID=Msa0972570-mRNA-1:cds;Parent=Msa0972570-mRNA-1
chr6_4 three_prime_UTR 4859723 4859975 4859723 ID=Msa0972570-mRNA-1:three_prime_utr;Parent=Msa0972570-mRNA-1
Gene Sequence

>Msa0972570

CTTTCTTGTACCGTTGTATTTGGTGCTGAACCAGCTGGTGCAGGAGCTTTACTGCTTGCTGATGATGGGAGTCAGTACTATGGATTCTGGCAAGGATTTGGGCATCAAACAAAAGAGGGGGCGCAGTATCGGGCGTTGATTTTAGGTATGAGACAGGCGATCATGAAAGGATTTAAACACGTTACCGTCAAAGGAGACTCTGAGTTTGTTGTCAATCAGTTTGGGGAACCCTGGCAGACCAACGACACACACTTAAGGAGCTTATGTCTGGAGGCTATGAAGTTGAGGAATAGCTTTGACTCATTCTCCATCCAGCACATTCCTCGGATGGTGAATATGACGAAGAACGCATCACTTGATGATCATCTGACAACATGA
Protein sequence

>Msa0972570

LSCTVVFGAEPAGAGALLLADDGSQYYGFWQGFGHQTKEGAQYRALILGMRQAIMKGFKHVTVKGDSEFVVNQFGEPWQTNDTHLRSLCLEAMKLRNSFDSFSIQHIPRMVNMTKNASLDDHLTT*