AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0972710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0972710 MtrunA17_Chr2g0325301 94.326 141 8 0 2 142 8 148 5.93e-95 270
Msa0972710 MtrunA17_Chr6g0451411 59.559 136 55 0 4 139 176 311 6.47e-52 167
Msa0972710 MtrunA17_Chr6g0451421 55.797 138 61 0 2 139 6 143 5.62e-51 159
Msa0972710 MtrunA17_Chr2g0329701 50.000 140 70 0 3 142 233 372 1.03e-44 150
Msa0972710 MtrunA17_Chr7g0264421 50.365 137 68 0 3 139 203 339 2.31e-44 148
Msa0972710 MtrunA17_Chr6g0451441 47.945 146 68 1 2 139 318 463 6.12e-37 131
Msa0972710 MtrunA17_Chr6g0451451 48.780 82 42 0 58 139 1 82 1.68e-23 87.4
Msa0972710 MtrunA17_Chr1g0158081 36.364 121 75 1 7 127 1209 1327 7.54e-16 73.2
Msa0972710 MtrunA17_Chr3g0110591 35.537 121 76 1 7 127 25 143 8.61e-15 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0972710 AT5G51080.3 53.285 137 64 0 3 139 121 257 1.37e-44 147
Msa0972710 AT3G01410.2 52.800 125 59 0 4 128 154 278 2.50e-44 147
Msa0972710 AT3G01410.1 52.800 125 59 0 4 128 154 278 2.50e-44 147
Msa0972710 AT5G51080.1 53.676 136 63 0 4 139 185 320 7.14e-44 146
Msa0972710 AT5G51080.2 53.676 136 63 0 4 139 185 320 7.14e-44 146
Msa0972710 AT1G24090.1 52.941 136 64 0 4 139 216 351 6.54e-41 140

Find 62 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATATAATGGTCTAATTT+TGG 0.114690 6_4:+5005148 Msa0972710:CDS
TTGCACAGGTCCCTTAAATT+TGG 0.134422 6_4:-5005568 None:intergenic
AAGTCAACAACCCAAATTTA+AGG 0.267370 6_4:+5005557 Msa0972710:CDS
ATATCCCACCATTGCTTTAG+AGG 0.272940 6_4:+5006474 Msa0972710:three_prime_UTR
TTCTATCTTCTGTCTTCATT+TGG 0.279448 6_4:+5006563 Msa0972710:three_prime_UTR
CAAAAGTTTAACAAATTTAT+CGG 0.281331 6_4:-5006694 None:intergenic
TGGATGCTCCATCGAATTCT+AGG 0.296997 6_4:-5004922 None:intergenic
ATTGCTTTAGAGGCTTAAAA+TGG 0.312456 6_4:+5006484 Msa0972710:three_prime_UTR
TGGTGCTCTGCTTTGATGTT+TGG 0.333585 6_4:+5006504 Msa0972710:three_prime_UTR
ATTTGGGTTGTTGACTTTCC+AGG 0.336100 6_4:-5005551 None:intergenic
TGCACAGGTCCCTTAAATTT+GGG 0.337238 6_4:-5005567 None:intergenic
TGTATGACCATGGTAGGTTT+TGG 0.348487 6_4:+5006647 Msa0972710:three_prime_UTR
AATAATTCTGCTGAATATAA+TGG 0.390357 6_4:+5005137 Msa0972710:CDS
TACATAAATGCTGAACTAAT+AGG 0.401874 6_4:-5006387 None:intergenic
GCTCCTGCACCAGACCTTCC+AGG 0.402463 6_4:-5004953 None:intergenic
TGCGTTCTGCAGATGGGAGT+CGG 0.417303 6_4:+5004981 Msa0972710:CDS
CTCTCAGCTTGTTTGCAAGC+AGG 0.424209 6_4:+5005223 Msa0972710:CDS
GCTGTTCTGCGTTCTGCAGA+TGG 0.444970 6_4:+5004974 Msa0972710:CDS
GGAGCATCCAGTGGAAATCC+TGG 0.466151 6_4:+5004935 Msa0972710:CDS
AGTGGAAATCCTGGAAGGTC+TGG 0.475520 6_4:+5004944 Msa0972710:CDS
CATGGTGTCAGCCAAGGACT+TGG 0.478840 6_4:+5005104 Msa0972710:CDS
AGTCAACAACCCAAATTTAA+GGG 0.482554 6_4:+5005558 Msa0972710:CDS
TTGTTTGAGTGCCAAGTCCT+TGG 0.498512 6_4:-5005115 None:intergenic
CATGAACATGCTCATATCCT+TGG 0.505728 6_4:-5005190 None:intergenic
TATGCCATGGCGAACATGAC+TGG 0.508165 6_4:+5006613 Msa0972710:three_prime_UTR
GCGTTCTGCAGATGGGAGTC+GGG 0.509020 6_4:+5004982 Msa0972710:CDS
AATGTCTCTTTATAGGTCCA+TGG 0.521374 6_4:+5005086 Msa0972710:intron
CTGTTCTGCGTTCTGCAGAT+GGG 0.528388 6_4:+5004975 Msa0972710:CDS
GTAGTAATATTCAACTTCGC+CGG 0.535597 6_4:-5006282 None:intergenic
TCCGTGTCGAACATGTTCCT+AGG 0.535759 6_4:+5005626 Msa0972710:CDS
TATGGGTGGTGATTGACCTA+CGG 0.541393 6_4:-5006732 None:intergenic
GCTCCACAGCCTCATTGCAC+AGG 0.545995 6_4:-5005582 None:intergenic
AGACCTTCCAGGATTTCCAC+TGG 0.556723 6_4:-5004942 None:intergenic
AATCCTGGAAGGTCTGGTGC+AGG 0.565510 6_4:+5004950 Msa0972710:CDS
GGACCTGTGCAATGAGGCTG+TGG 0.567095 6_4:+5005579 Msa0972710:CDS
TAATATCTTGTGATATGCCA+TGG 0.567729 6_4:+5006600 Msa0972710:three_prime_UTR
GCATGCTGTCAGTCTGTCAC+AGG 0.567941 6_4:-5006309 None:intergenic
TTAAGCCTCTAAAGCAATGG+TGG 0.569232 6_4:-5006479 None:intergenic
CATAAAACCAAAACCTACCA+TGG 0.584026 6_4:-5006654 None:intergenic
CTGAAAGAAGCTAGCAACCA+AGG 0.584237 6_4:+5005173 Msa0972710:CDS
CCGTGTCGAACATGTTCCTA+GGG 0.585847 6_4:+5005627 Msa0972710:CDS
TAGGTCCATGGTGTCAGCCA+AGG 0.586553 6_4:+5005098 Msa0972710:intron
TATGTAACACTGCATGTCAA+AGG 0.587917 6_4:+5006404 Msa0972710:three_prime_UTR
TCTTGTATCCTAGAATTCGA+TGG 0.589857 6_4:+5004914 Msa0972710:CDS
GTTTAATGTATGACCATGGT+AGG 0.593786 6_4:+5006641 Msa0972710:three_prime_UTR
TAAGCCTCTAAAGCAATGGT+GGG 0.598715 6_4:-5006478 None:intergenic
ATGGTGGGATATACACATCA+TGG 0.603290 6_4:-5006463 None:intergenic
TTGGATTTGCAACAGTTTGG+GGG 0.607031 6_4:+5005526 Msa0972710:intron
CAAGTCCTTGGCTGACACCA+TGG 0.609890 6_4:-5005103 None:intergenic
GAGCAAACTGAGAGAACCGT+AGG 0.613306 6_4:+5006716 Msa0972710:three_prime_UTR
CCCTAGGAACATGTTCGACA+CGG 0.624380 6_4:-5005627 None:intergenic
CCATCCAGTCATGTTCGCCA+TGG 0.629927 6_4:-5006617 None:intergenic
TATGAGCATGTTCATGTCCG+AGG 0.645245 6_4:+5005197 Msa0972710:CDS
ACTGTCAAACTATTGGCAAG+TGG 0.649315 6_4:+5006333 Msa0972710:intron
GAATTCGATGGAGCATCCAG+TGG 0.650462 6_4:+5004926 Msa0972710:CDS
CATCCAGTGGAAATCCTGGA+AGG 0.657104 6_4:+5004939 Msa0972710:CDS
GCTGATTCCCAAGCAAATCG+CGG 0.659442 6_4:+5005998 Msa0972710:CDS
TTTAAGGGACCTGTGCAATG+AGG 0.663669 6_4:+5005573 Msa0972710:CDS
CCATGGCGAACATGACTGGA+TGG 0.671111 6_4:+5006617 Msa0972710:three_prime_UTR
AAACAAGCTGAGAGTCACCT+CGG 0.700596 6_4:-5005214 None:intergenic
TGGTGTTTAATGTATGACCA+TGG 0.742844 6_4:+5006637 Msa0972710:three_prime_UTR
GAAATTCAGAAACATACACA+CGG 0.744973 6_4:-5006543 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAATATTAAAATAAAAC+AGG - chr6_4:5006203-5006222 None:intergenic 10.0%
!!! TTATTTTAATATTTTGTCTT+TGG + chr6_4:5006206-5006225 Msa0972710:intron 10.0%
!!! TATTTTAATATTTTGTCTTT+GGG + chr6_4:5006207-5006226 Msa0972710:intron 10.0%
!! ACAAAATATTAAAATAAAAC+AGG - chr6_4:5006203-5006222 None:intergenic 10.0%
!!! TTATTTTAATATTTTGTCTT+TGG + chr6_4:5006206-5006225 Msa0972710:intron 10.0%
!!! TATTTTAATATTTTGTCTTT+GGG + chr6_4:5006207-5006226 Msa0972710:intron 10.0%
!! TGTAATATTTAGTGAATAAT+TGG - chr6_4:5005356-5005375 None:intergenic 15.0%
!! ATTATTCACTAAATATTACA+CGG + chr6_4:5005356-5005375 Msa0972710:intron 15.0%
!!! ATTTACCATCATATTTTTAT+TGG + chr6_4:5005507-5005526 Msa0972710:intron 15.0%
!! CAAAAGTTTAACAAATTTAT+CGG - chr6_4:5006697-5006716 None:intergenic 15.0%
!! TGTAATATTTAGTGAATAAT+TGG - chr6_4:5005356-5005375 None:intergenic 15.0%
!! ATTATTCACTAAATATTACA+CGG + chr6_4:5005356-5005375 Msa0972710:intron 15.0%
!!! ATTTACCATCATATTTTTAT+TGG + chr6_4:5005507-5005526 Msa0972710:intron 15.0%
!! CAAAAGTTTAACAAATTTAT+CGG - chr6_4:5006697-5006716 None:intergenic 15.0%
!! ATAATGCAAAAATGATTTGA+TGG - chr6_4:5005020-5005039 None:intergenic 20.0%
!! TGCATTATACAATATATCAA+AGG + chr6_4:5005051-5005070 Msa0972710:intron 20.0%
!! AATAATTCTGCTGAATATAA+TGG + chr6_4:5005137-5005156 Msa0972710:CDS 20.0%
!!! TGAATATAATGGTCTAATTT+TGG + chr6_4:5005148-5005167 Msa0972710:CDS 20.0%
!!! GAATATAATGGTCTAATTTT+GGG + chr6_4:5005149-5005168 Msa0972710:CDS 20.0%
!!! AATATAATGGTCTAATTTTG+GGG + chr6_4:5005150-5005169 Msa0972710:CDS 20.0%
!! CAAATCCAATAAAAATATGA+TGG - chr6_4:5005515-5005534 None:intergenic 20.0%
!!! TCATGTTTTTTTATTGTTGA+AGG + chr6_4:5005961-5005980 Msa0972710:intron 20.0%
!!! CATGTTTTTTTATTGTTGAA+GGG + chr6_4:5005962-5005981 Msa0972710:intron 20.0%
!!! TTATTGCTATTTCATTTACT+AGG - chr6_4:5006091-5006110 None:intergenic 20.0%
!!! TTTTTCTTTTAAAAGAGCTT+TGG - chr6_4:5006121-5006140 None:intergenic 20.0%
!!! TTTTTTTTTTTTTTTGCAGC+CGG + chr6_4:5006263-5006282 Msa0972710:intron 20.0%
!!! CTATGTTAATTTTCTTGTTT+AGG + chr6_4:5006359-5006378 Msa0972710:three_prime_UTR 20.0%
!! ATAATGCAAAAATGATTTGA+TGG - chr6_4:5005020-5005039 None:intergenic 20.0%
!! TGCATTATACAATATATCAA+AGG + chr6_4:5005051-5005070 Msa0972710:intron 20.0%
!! AATAATTCTGCTGAATATAA+TGG + chr6_4:5005137-5005156 Msa0972710:CDS 20.0%
!!! TGAATATAATGGTCTAATTT+TGG + chr6_4:5005148-5005167 Msa0972710:CDS 20.0%
!!! GAATATAATGGTCTAATTTT+GGG + chr6_4:5005149-5005168 Msa0972710:CDS 20.0%
!!! AATATAATGGTCTAATTTTG+GGG + chr6_4:5005150-5005169 Msa0972710:CDS 20.0%
!! CAAATCCAATAAAAATATGA+TGG - chr6_4:5005515-5005534 None:intergenic 20.0%
!!! TCATGTTTTTTTATTGTTGA+AGG + chr6_4:5005961-5005980 Msa0972710:intron 20.0%
!!! CATGTTTTTTTATTGTTGAA+GGG + chr6_4:5005962-5005981 Msa0972710:intron 20.0%
!!! TTATTGCTATTTCATTTACT+AGG - chr6_4:5006091-5006110 None:intergenic 20.0%
!!! TTTTTCTTTTAAAAGAGCTT+TGG - chr6_4:5006121-5006140 None:intergenic 20.0%
!!! TTTTTTTTTTTTTTTGCAGC+CGG + chr6_4:5006263-5006282 Msa0972710:intron 20.0%
!!! CTATGTTAATTTTCTTGTTT+AGG + chr6_4:5006359-5006378 Msa0972710:three_prime_UTR 20.0%
! AACACTTAATGTCTCTTTAT+AGG + chr6_4:5005079-5005098 Msa0972710:intron 25.0%
!!! AAAGTAGTCTTAACCAATTT+TGG + chr6_4:5005266-5005285 Msa0972710:intron 25.0%
! TATAGTTTCTACATGAACAT+TGG + chr6_4:5005402-5005421 Msa0972710:intron 25.0%
!!! AAACATCTTTCACTTTTGTT+TGG + chr6_4:5006143-5006162 Msa0972710:intron 25.0%
!! TTAATATTTTGTCTTTGGGT+AGG + chr6_4:5006211-5006230 Msa0972710:intron 25.0%
!! TAATATTTTGTCTTTGGGTA+GGG + chr6_4:5006212-5006231 Msa0972710:intron 25.0%
! TACATAAATGCTGAACTAAT+AGG - chr6_4:5006390-5006409 None:intergenic 25.0%
! AACACTTAATGTCTCTTTAT+AGG + chr6_4:5005079-5005098 Msa0972710:intron 25.0%
!!! AAAGTAGTCTTAACCAATTT+TGG + chr6_4:5005266-5005285 Msa0972710:intron 25.0%
! TATAGTTTCTACATGAACAT+TGG + chr6_4:5005402-5005421 Msa0972710:intron 25.0%
!!! AAACATCTTTCACTTTTGTT+TGG + chr6_4:5006143-5006162 Msa0972710:intron 25.0%
!! TTAATATTTTGTCTTTGGGT+AGG + chr6_4:5006211-5006230 Msa0972710:intron 25.0%
!! TAATATTTTGTCTTTGGGTA+GGG + chr6_4:5006212-5006231 Msa0972710:intron 25.0%
! TACATAAATGCTGAACTAAT+AGG - chr6_4:5006390-5006409 None:intergenic 25.0%
! GACTACTTTGTAACTTGTAA+GGG - chr6_4:5005255-5005274 None:intergenic 30.0%
! AGACTACTTTGTAACTTGTA+AGG - chr6_4:5005256-5005275 None:intergenic 30.0%
GGTGAATTATAGACCAAAAT+TGG - chr6_4:5005282-5005301 None:intergenic 30.0%
TCATTCAGATGCAATTTAAG+TGG - chr6_4:5005303-5005322 None:intergenic 30.0%
TTATTGGATTTGCAACAGTT+TGG + chr6_4:5005523-5005542 Msa0972710:intron 30.0%
TATTGGATTTGCAACAGTTT+GGG + chr6_4:5005524-5005543 Msa0972710:intron 30.0%
AAGTCAACAACCCAAATTTA+AGG + chr6_4:5005557-5005576 Msa0972710:CDS 30.0%
AGTCAACAACCCAAATTTAA+GGG + chr6_4:5005558-5005577 Msa0972710:CDS 30.0%
!! TTTGCTCTGGAAAACTAAAA+AGG + chr6_4:5005735-5005754 Msa0972710:intron 30.0%
TGTGATCCATATGCTTTAAT+AGG + chr6_4:5005854-5005873 Msa0972710:intron 30.0%
! GTTTTGCCTATTAAAGCATA+TGG - chr6_4:5005863-5005882 None:intergenic 30.0%
!! TCACTTTTGTTTGGTTGTAA+TGG + chr6_4:5006152-5006171 Msa0972710:intron 30.0%
!!! ATTTTAAGCCTCTAAAGCAA+TGG - chr6_4:5006485-5006504 None:intergenic 30.0%
!! ATTGCTTTAGAGGCTTAAAA+TGG + chr6_4:5006484-5006503 Msa0972710:three_prime_UTR 30.0%
! TGATGTTTGGATTTTATGCT+TGG + chr6_4:5006517-5006536 Msa0972710:three_prime_UTR 30.0%
GAAATTCAGAAACATACACA+CGG - chr6_4:5006546-5006565 None:intergenic 30.0%
TTCTATCTTCTGTCTTCATT+TGG + chr6_4:5006563-5006582 Msa0972710:three_prime_UTR 30.0%
TAATATCTTGTGATATGCCA+TGG + chr6_4:5006600-5006619 Msa0972710:three_prime_UTR 30.0%
!!! AGGTTTTGGTTTTATGCAAT+TGG + chr6_4:5006661-5006680 Msa0972710:three_prime_UTR 30.0%
! GACTACTTTGTAACTTGTAA+GGG - chr6_4:5005255-5005274 None:intergenic 30.0%
! AGACTACTTTGTAACTTGTA+AGG - chr6_4:5005256-5005275 None:intergenic 30.0%
GGTGAATTATAGACCAAAAT+TGG - chr6_4:5005282-5005301 None:intergenic 30.0%
TCATTCAGATGCAATTTAAG+TGG - chr6_4:5005303-5005322 None:intergenic 30.0%
TTATTGGATTTGCAACAGTT+TGG + chr6_4:5005523-5005542 Msa0972710:intron 30.0%
TATTGGATTTGCAACAGTTT+GGG + chr6_4:5005524-5005543 Msa0972710:intron 30.0%
AAGTCAACAACCCAAATTTA+AGG + chr6_4:5005557-5005576 Msa0972710:CDS 30.0%
AGTCAACAACCCAAATTTAA+GGG + chr6_4:5005558-5005577 Msa0972710:CDS 30.0%
!! TTTGCTCTGGAAAACTAAAA+AGG + chr6_4:5005735-5005754 Msa0972710:intron 30.0%
TGTGATCCATATGCTTTAAT+AGG + chr6_4:5005854-5005873 Msa0972710:intron 30.0%
! GTTTTGCCTATTAAAGCATA+TGG - chr6_4:5005863-5005882 None:intergenic 30.0%
!! TCACTTTTGTTTGGTTGTAA+TGG + chr6_4:5006152-5006171 Msa0972710:intron 30.0%
!!! ATTTTAAGCCTCTAAAGCAA+TGG - chr6_4:5006485-5006504 None:intergenic 30.0%
!! ATTGCTTTAGAGGCTTAAAA+TGG + chr6_4:5006484-5006503 Msa0972710:three_prime_UTR 30.0%
! TGATGTTTGGATTTTATGCT+TGG + chr6_4:5006517-5006536 Msa0972710:three_prime_UTR 30.0%
GAAATTCAGAAACATACACA+CGG - chr6_4:5006546-5006565 None:intergenic 30.0%
TTCTATCTTCTGTCTTCATT+TGG + chr6_4:5006563-5006582 Msa0972710:three_prime_UTR 30.0%
TAATATCTTGTGATATGCCA+TGG + chr6_4:5006600-5006619 Msa0972710:three_prime_UTR 30.0%
!!! AGGTTTTGGTTTTATGCAAT+TGG + chr6_4:5006661-5006680 Msa0972710:three_prime_UTR 30.0%
TCTTGTATCCTAGAATTCGA+TGG + chr6_4:5004914-5004933 Msa0972710:CDS 35.0%
AATGTCTCTTTATAGGTCCA+TGG + chr6_4:5005086-5005105 Msa0972710:intron 35.0%
TCTACATGAACATTGGTGTA+AGG + chr6_4:5005409-5005428 Msa0972710:intron 35.0%
AATTCATTCCTGCCTTTGTT+CGG + chr6_4:5005440-5005459 Msa0972710:intron 35.0%
ATTGGATTTGCAACAGTTTG+GGG + chr6_4:5005525-5005544 Msa0972710:intron 35.0%
GACAAACATGTTCTTCATTG+TGG - chr6_4:5005690-5005709 None:intergenic 35.0%
AGTGTAATTGTTCTCAACCT+CGG - chr6_4:5005761-5005780 None:intergenic 35.0%
! AGACTTTTCTGAGTTCTTGT+AGG + chr6_4:5006048-5006067 Msa0972710:intron 35.0%
GTAGTAATATTCAACTTCGC+CGG - chr6_4:5006285-5006304 None:intergenic 35.0%
TATGTAACACTGCATGTCAA+AGG + chr6_4:5006404-5006423 Msa0972710:three_prime_UTR 35.0%
TGGTGTTTAATGTATGACCA+TGG + chr6_4:5006637-5006656 Msa0972710:three_prime_UTR 35.0%
GTTTAATGTATGACCATGGT+AGG + chr6_4:5006641-5006660 Msa0972710:three_prime_UTR 35.0%
CATAAAACCAAAACCTACCA+TGG - chr6_4:5006657-5006676 None:intergenic 35.0%
TCTTGTATCCTAGAATTCGA+TGG + chr6_4:5004914-5004933 Msa0972710:CDS 35.0%
AATGTCTCTTTATAGGTCCA+TGG + chr6_4:5005086-5005105 Msa0972710:intron 35.0%
TCTACATGAACATTGGTGTA+AGG + chr6_4:5005409-5005428 Msa0972710:intron 35.0%
AATTCATTCCTGCCTTTGTT+CGG + chr6_4:5005440-5005459 Msa0972710:intron 35.0%
ATTGGATTTGCAACAGTTTG+GGG + chr6_4:5005525-5005544 Msa0972710:intron 35.0%
GACAAACATGTTCTTCATTG+TGG - chr6_4:5005690-5005709 None:intergenic 35.0%
AGTGTAATTGTTCTCAACCT+CGG - chr6_4:5005761-5005780 None:intergenic 35.0%
! AGACTTTTCTGAGTTCTTGT+AGG + chr6_4:5006048-5006067 Msa0972710:intron 35.0%
GTAGTAATATTCAACTTCGC+CGG - chr6_4:5006285-5006304 None:intergenic 35.0%
TATGTAACACTGCATGTCAA+AGG + chr6_4:5006404-5006423 Msa0972710:three_prime_UTR 35.0%
TGGTGTTTAATGTATGACCA+TGG + chr6_4:5006637-5006656 Msa0972710:three_prime_UTR 35.0%
GTTTAATGTATGACCATGGT+AGG + chr6_4:5006641-5006660 Msa0972710:three_prime_UTR 35.0%
CATAAAACCAAAACCTACCA+TGG - chr6_4:5006657-5006676 None:intergenic 35.0%
CATGAACATGCTCATATCCT+TGG - chr6_4:5005193-5005212 None:intergenic 40.0%
TTGGATTTGCAACAGTTTGG+GGG + chr6_4:5005526-5005545 Msa0972710:intron 40.0%
!! ATTTGGGTTGTTGACTTTCC+AGG - chr6_4:5005554-5005573 None:intergenic 40.0%
TGCACAGGTCCCTTAAATTT+GGG - chr6_4:5005570-5005589 None:intergenic 40.0%
TTGCACAGGTCCCTTAAATT+TGG - chr6_4:5005571-5005590 None:intergenic 40.0%
TGTGACAAAGTGTATACCCT+AGG - chr6_4:5005646-5005665 None:intergenic 40.0%
GAGCAAAACACAATCAAGAG+AGG - chr6_4:5005722-5005741 None:intergenic 40.0%
! CTCTTGATTGTGTTTTGCTC+TGG + chr6_4:5005722-5005741 Msa0972710:intron 40.0%
! GAGAGGCATGCGAATTAAAA+AGG - chr6_4:5005823-5005842 None:intergenic 40.0%
ATCACATGAGTTACGTAGAG+AGG - chr6_4:5005840-5005859 None:intergenic 40.0%
!! ATTTTTGCCGCGATTTGCTT+GGG - chr6_4:5006008-5006027 None:intergenic 40.0%
GCATGCTACTGTCAAACTAT+TGG + chr6_4:5006326-5006345 Msa0972710:intron 40.0%
ACTGTCAAACTATTGGCAAG+TGG + chr6_4:5006333-5006352 Msa0972710:intron 40.0%
! ATGGTGGGATATACACATCA+TGG - chr6_4:5006466-5006485 None:intergenic 40.0%
ATATCCCACCATTGCTTTAG+AGG + chr6_4:5006474-5006493 Msa0972710:three_prime_UTR 40.0%
!! TAAGCCTCTAAAGCAATGGT+GGG - chr6_4:5006481-5006500 None:intergenic 40.0%
!! TTAAGCCTCTAAAGCAATGG+TGG - chr6_4:5006482-5006501 None:intergenic 40.0%
! TGTATGACCATGGTAGGTTT+TGG + chr6_4:5006647-5006666 Msa0972710:three_prime_UTR 40.0%
CATGAACATGCTCATATCCT+TGG - chr6_4:5005193-5005212 None:intergenic 40.0%
TTGGATTTGCAACAGTTTGG+GGG + chr6_4:5005526-5005545 Msa0972710:intron 40.0%
!! ATTTGGGTTGTTGACTTTCC+AGG - chr6_4:5005554-5005573 None:intergenic 40.0%
TGCACAGGTCCCTTAAATTT+GGG - chr6_4:5005570-5005589 None:intergenic 40.0%
TTGCACAGGTCCCTTAAATT+TGG - chr6_4:5005571-5005590 None:intergenic 40.0%
TGTGACAAAGTGTATACCCT+AGG - chr6_4:5005646-5005665 None:intergenic 40.0%
GAGCAAAACACAATCAAGAG+AGG - chr6_4:5005722-5005741 None:intergenic 40.0%
! CTCTTGATTGTGTTTTGCTC+TGG + chr6_4:5005722-5005741 Msa0972710:intron 40.0%
! GAGAGGCATGCGAATTAAAA+AGG - chr6_4:5005823-5005842 None:intergenic 40.0%
ATCACATGAGTTACGTAGAG+AGG - chr6_4:5005840-5005859 None:intergenic 40.0%
!! ATTTTTGCCGCGATTTGCTT+GGG - chr6_4:5006008-5006027 None:intergenic 40.0%
GCATGCTACTGTCAAACTAT+TGG + chr6_4:5006326-5006345 Msa0972710:intron 40.0%
ACTGTCAAACTATTGGCAAG+TGG + chr6_4:5006333-5006352 Msa0972710:intron 40.0%
! ATGGTGGGATATACACATCA+TGG - chr6_4:5006466-5006485 None:intergenic 40.0%
ATATCCCACCATTGCTTTAG+AGG + chr6_4:5006474-5006493 Msa0972710:three_prime_UTR 40.0%
!! TAAGCCTCTAAAGCAATGGT+GGG - chr6_4:5006481-5006500 None:intergenic 40.0%
!! TTAAGCCTCTAAAGCAATGG+TGG - chr6_4:5006482-5006501 None:intergenic 40.0%
! TGTATGACCATGGTAGGTTT+TGG + chr6_4:5006647-5006666 Msa0972710:three_prime_UTR 40.0%
TGGATGCTCCATCGAATTCT+AGG - chr6_4:5004925-5004944 None:intergenic 45.0%
TTGTTTGAGTGCCAAGTCCT+TGG - chr6_4:5005118-5005137 None:intergenic 45.0%
CTGAAAGAAGCTAGCAACCA+AGG + chr6_4:5005173-5005192 Msa0972710:CDS 45.0%
TATGAGCATGTTCATGTCCG+AGG + chr6_4:5005197-5005216 Msa0972710:CDS 45.0%
AAACAAGCTGAGAGTCACCT+CGG - chr6_4:5005217-5005236 None:intergenic 45.0%
CAACCACTGAATCCGAACAA+AGG - chr6_4:5005455-5005474 None:intergenic 45.0%
CTTTGTTCGGATTCAGTGGT+TGG + chr6_4:5005453-5005472 Msa0972710:intron 45.0%
TTTAAGGGACCTGTGCAATG+AGG + chr6_4:5005573-5005592 Msa0972710:CDS 45.0%
CTGGAAAACTAAAAAGGCCG+AGG + chr6_4:5005741-5005760 Msa0972710:intron 45.0%
!! GATTTTTGCCGCGATTTGCT+TGG - chr6_4:5006009-5006028 None:intergenic 45.0%
GCAAATCGCGGCAAAAATCA+TGG + chr6_4:5006010-5006029 Msa0972710:CDS 45.0%
AATCGCGGCAAAAATCATGG+AGG + chr6_4:5006013-5006032 Msa0972710:CDS 45.0%
TGGTGCTCTGCTTTGATGTT+TGG + chr6_4:5006504-5006523 Msa0972710:three_prime_UTR 45.0%
AGGATACAAGAACGCTGCAA+TGG - chr6_4:5004905-5004924 None:intergenic 45.0%
TGGATGCTCCATCGAATTCT+AGG - chr6_4:5004925-5004944 None:intergenic 45.0%
TTGTTTGAGTGCCAAGTCCT+TGG - chr6_4:5005118-5005137 None:intergenic 45.0%
CTGAAAGAAGCTAGCAACCA+AGG + chr6_4:5005173-5005192 Msa0972710:CDS 45.0%
TATGAGCATGTTCATGTCCG+AGG + chr6_4:5005197-5005216 Msa0972710:CDS 45.0%
AAACAAGCTGAGAGTCACCT+CGG - chr6_4:5005217-5005236 None:intergenic 45.0%
CAACCACTGAATCCGAACAA+AGG - chr6_4:5005455-5005474 None:intergenic 45.0%
CTTTGTTCGGATTCAGTGGT+TGG + chr6_4:5005453-5005472 Msa0972710:intron 45.0%
TTTAAGGGACCTGTGCAATG+AGG + chr6_4:5005573-5005592 Msa0972710:CDS 45.0%
CTGGAAAACTAAAAAGGCCG+AGG + chr6_4:5005741-5005760 Msa0972710:intron 45.0%
!! GATTTTTGCCGCGATTTGCT+TGG - chr6_4:5006009-5006028 None:intergenic 45.0%
GCAAATCGCGGCAAAAATCA+TGG + chr6_4:5006010-5006029 Msa0972710:CDS 45.0%
AATCGCGGCAAAAATCATGG+AGG + chr6_4:5006013-5006032 Msa0972710:CDS 45.0%
TGGTGCTCTGCTTTGATGTT+TGG + chr6_4:5006504-5006523 Msa0972710:three_prime_UTR 45.0%
! GAATTCGATGGAGCATCCAG+TGG + chr6_4:5004926-5004945 Msa0972710:CDS 50.0%
CATCCAGTGGAAATCCTGGA+AGG + chr6_4:5004939-5004958 Msa0972710:CDS 50.0%
AGACCTTCCAGGATTTCCAC+TGG - chr6_4:5004945-5004964 None:intergenic 50.0%
AGTGGAAATCCTGGAAGGTC+TGG + chr6_4:5004944-5004963 Msa0972710:CDS 50.0%
CTGTTCTGCGTTCTGCAGAT+GGG + chr6_4:5004975-5004994 Msa0972710:CDS 50.0%
! CTCTCAGCTTGTTTGCAAGC+AGG + chr6_4:5005223-5005242 Msa0972710:CDS 50.0%
CACTGAATCCGAACAAAGGC+AGG - chr6_4:5005451-5005470 None:intergenic 50.0%
CTGCCTTTGTTCGGATTCAG+TGG + chr6_4:5005449-5005468 Msa0972710:intron 50.0%
TCCGTGTCGAACATGTTCCT+AGG + chr6_4:5005626-5005645 Msa0972710:CDS 50.0%
CCCTAGGAACATGTTCGACA+CGG - chr6_4:5005630-5005649 None:intergenic 50.0%
CCGTGTCGAACATGTTCCTA+GGG + chr6_4:5005627-5005646 Msa0972710:CDS 50.0%
! GCTGATTCCCAAGCAAATCG+CGG + chr6_4:5005998-5006017 Msa0972710:CDS 50.0%
TATGCCATGGCGAACATGAC+TGG + chr6_4:5006613-5006632 Msa0972710:three_prime_UTR 50.0%
GAGCAAACTGAGAGAACCGT+AGG + chr6_4:5006716-5006735 Msa0972710:three_prime_UTR 50.0%
! GAATTCGATGGAGCATCCAG+TGG + chr6_4:5004926-5004945 Msa0972710:CDS 50.0%
CATCCAGTGGAAATCCTGGA+AGG + chr6_4:5004939-5004958 Msa0972710:CDS 50.0%
AGACCTTCCAGGATTTCCAC+TGG - chr6_4:5004945-5004964 None:intergenic 50.0%
AGTGGAAATCCTGGAAGGTC+TGG + chr6_4:5004944-5004963 Msa0972710:CDS 50.0%
CTGTTCTGCGTTCTGCAGAT+GGG + chr6_4:5004975-5004994 Msa0972710:CDS 50.0%
! CTCTCAGCTTGTTTGCAAGC+AGG + chr6_4:5005223-5005242 Msa0972710:CDS 50.0%
CACTGAATCCGAACAAAGGC+AGG - chr6_4:5005451-5005470 None:intergenic 50.0%
CTGCCTTTGTTCGGATTCAG+TGG + chr6_4:5005449-5005468 Msa0972710:intron 50.0%
TCCGTGTCGAACATGTTCCT+AGG + chr6_4:5005626-5005645 Msa0972710:CDS 50.0%
CCCTAGGAACATGTTCGACA+CGG - chr6_4:5005630-5005649 None:intergenic 50.0%
CCGTGTCGAACATGTTCCTA+GGG + chr6_4:5005627-5005646 Msa0972710:CDS 50.0%
! GCTGATTCCCAAGCAAATCG+CGG + chr6_4:5005998-5006017 Msa0972710:CDS 50.0%
TATGCCATGGCGAACATGAC+TGG + chr6_4:5006613-5006632 Msa0972710:three_prime_UTR 50.0%
GAGCAAACTGAGAGAACCGT+AGG + chr6_4:5006716-5006735 Msa0972710:three_prime_UTR 50.0%
! GGAGCATCCAGTGGAAATCC+TGG + chr6_4:5004935-5004954 Msa0972710:CDS 55.0%
AATCCTGGAAGGTCTGGTGC+AGG + chr6_4:5004950-5004969 Msa0972710:CDS 55.0%
GCTGTTCTGCGTTCTGCAGA+TGG + chr6_4:5004974-5004993 Msa0972710:CDS 55.0%
TGCGTTCTGCAGATGGGAGT+CGG + chr6_4:5004981-5005000 Msa0972710:CDS 55.0%
! TAGGTCCATGGTGTCAGCCA+AGG + chr6_4:5005098-5005117 Msa0972710:intron 55.0%
CAAGTCCTTGGCTGACACCA+TGG - chr6_4:5005106-5005125 None:intergenic 55.0%
! CATGGTGTCAGCCAAGGACT+TGG + chr6_4:5005104-5005123 Msa0972710:CDS 55.0%
TGCAACAGTTTGGGGGTTCC+TGG + chr6_4:5005533-5005552 Msa0972710:intron 55.0%
GCATGCTGTCAGTCTGTCAC+AGG - chr6_4:5006312-5006331 None:intergenic 55.0%
CCATCCAGTCATGTTCGCCA+TGG - chr6_4:5006620-5006639 None:intergenic 55.0%
CCATGGCGAACATGACTGGA+TGG + chr6_4:5006617-5006636 Msa0972710:three_prime_UTR 55.0%
! GGAGCATCCAGTGGAAATCC+TGG + chr6_4:5004935-5004954 Msa0972710:CDS 55.0%
AATCCTGGAAGGTCTGGTGC+AGG + chr6_4:5004950-5004969 Msa0972710:CDS 55.0%
GCTGTTCTGCGTTCTGCAGA+TGG + chr6_4:5004974-5004993 Msa0972710:CDS 55.0%
TGCGTTCTGCAGATGGGAGT+CGG + chr6_4:5004981-5005000 Msa0972710:CDS 55.0%
! TAGGTCCATGGTGTCAGCCA+AGG + chr6_4:5005098-5005117 Msa0972710:intron 55.0%
CAAGTCCTTGGCTGACACCA+TGG - chr6_4:5005106-5005125 None:intergenic 55.0%
! CATGGTGTCAGCCAAGGACT+TGG + chr6_4:5005104-5005123 Msa0972710:CDS 55.0%
TGCAACAGTTTGGGGGTTCC+TGG + chr6_4:5005533-5005552 Msa0972710:intron 55.0%
GCATGCTGTCAGTCTGTCAC+AGG - chr6_4:5006312-5006331 None:intergenic 55.0%
CCATCCAGTCATGTTCGCCA+TGG - chr6_4:5006620-5006639 None:intergenic 55.0%
CCATGGCGAACATGACTGGA+TGG + chr6_4:5006617-5006636 Msa0972710:three_prime_UTR 55.0%
GCGTTCTGCAGATGGGAGTC+GGG + chr6_4:5004982-5005001 Msa0972710:CDS 60.0%
GGACCTGTGCAATGAGGCTG+TGG + chr6_4:5005579-5005598 Msa0972710:CDS 60.0%
GCTCCACAGCCTCATTGCAC+AGG - chr6_4:5005585-5005604 None:intergenic 60.0%
GCGTTCTGCAGATGGGAGTC+GGG + chr6_4:5004982-5005001 Msa0972710:CDS 60.0%
GGACCTGTGCAATGAGGCTG+TGG + chr6_4:5005579-5005598 Msa0972710:CDS 60.0%
GCTCCACAGCCTCATTGCAC+AGG - chr6_4:5005585-5005604 None:intergenic 60.0%
GCTCCTGCACCAGACCTTCC+AGG - chr6_4:5004956-5004975 None:intergenic 65.0%
GCTCCTGCACCAGACCTTCC+AGG - chr6_4:5004956-5004975 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 5004905 5006748 5004905 ID=Msa0972710;Name=Msa0972710
chr6_4 mRNA 5004905 5006748 5004905 ID=Msa0972710-mRNA-1;Parent=Msa0972710;Name=Msa0972710-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|1|0.83|1|0.8|0.66|6|421|142
chr6_4 exon 5004905 5005003 5004905 ID=Msa0972710-mRNA-1:exon:2174;Parent=Msa0972710-mRNA-1
chr6_4 exon 5005101 5005244 5005101 ID=Msa0972710-mRNA-1:exon:2175;Parent=Msa0972710-mRNA-1
chr6_4 exon 5005541 5005648 5005541 ID=Msa0972710-mRNA-1:exon:2176;Parent=Msa0972710-mRNA-1
chr6_4 exon 5005983 5006034 5005983 ID=Msa0972710-mRNA-1:exon:2177;Parent=Msa0972710-mRNA-1
chr6_4 exon 5006282 5006321 5006282 ID=Msa0972710-mRNA-1:exon:2178;Parent=Msa0972710-mRNA-1
chr6_4 exon 5006342 5006748 5006342 ID=Msa0972710-mRNA-1:exon:2179;Parent=Msa0972710-mRNA-1
chr6_4 CDS 5004905 5005003 5004905 ID=Msa0972710-mRNA-1:cds;Parent=Msa0972710-mRNA-1
chr6_4 CDS 5005101 5005244 5005101 ID=Msa0972710-mRNA-1:cds;Parent=Msa0972710-mRNA-1
chr6_4 CDS 5005541 5005648 5005541 ID=Msa0972710-mRNA-1:cds;Parent=Msa0972710-mRNA-1
chr6_4 CDS 5005983 5006034 5005983 ID=Msa0972710-mRNA-1:cds;Parent=Msa0972710-mRNA-1
chr6_4 CDS 5006282 5006307 5006282 ID=Msa0972710-mRNA-1:cds;Parent=Msa0972710-mRNA-1
chr6_4 three_prime_UTR 5006308 5006321 5006308 ID=Msa0972710-mRNA-1:three_prime_utr;Parent=Msa0972710-mRNA-1
chr6_4 three_prime_UTR 5006342 5006748 5006342 ID=Msa0972710-mRNA-1:three_prime_utr;Parent=Msa0972710-mRNA-1
Gene Sequence

>Msa0972710

TTGCAGCGTTCTTGTATCCTAGAATTCGATGGAGCATCCAGTGGAAATCCTGGAAGGTCTGGTGCAGGAGCTGTTCTGCGTTCTGCAGATGGGAGTCGGGTCCATGGTGTCAGCCAAGGACTTGGCACTCAAACAAATAATTCTGCTGAATATAATGGTCTAATTTTGGGGCTGAAAGAAGCTAGCAACCAAGGATATGAGCATGTTCATGTCCGAGGTGACTCTCAGCTTGTTTGCAAGCAGTTTGGGGGTTCCTGGAAAGTCAACAACCCAAATTTAAGGGACCTGTGCAATGAGGCTGTGGAGCTGAAGAGTAACTTCAAGTCAGTCCGTGTCGAACATGTTCCTAGGGGTTCTAACTCTGCAGCTGATTCCCAAGCAAATCGCGGCAAAAATCATGGAGCCGGCGAAGTTGAATATTACTACTAA
Protein sequence

>Msa0972710

LQRSCILEFDGASSGNPGRSGAGAVLRSADGSRVHGVSQGLGTQTNNSAEYNGLILGLKEASNQGYEHVHVRGDSQLVCKQFGGSWKVNNPNLRDLCNEAVELKSNFKSVRVEHVPRGSNSAADSQANRGKNHGAGEVEYYY*