AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0980680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACCTATTATTTCCCTAAA+TGG 0.196608 6_4:+19214283 Msa0980680:CDS
TTGCTGGCGGGGTTGCTTTA+AGG 0.196610 6_4:+19212583 Msa0980680:five_prime_UTR
CAGGAAGAGATGCTCCATTT+AGG 0.230401 6_4:-19214297 None:intergenic
TAGCAGAACCCATTTGAAAT+TGG 0.276955 6_4:+19212977 Msa0980680:five_prime_UTR
AACACTTCAACAGTATAAAA+AGG 0.319624 6_4:-19212676 None:intergenic
CATCAAAACTAAGGATAGTT+TGG 0.331399 6_4:+19212727 Msa0980680:five_prime_UTR
CTTTGCCTTCACACTATAAT+CGG 0.346969 6_4:-19212851 None:intergenic
AGGAAGAGATGCTCCATTTA+GGG 0.350989 6_4:-19214296 None:intergenic
CATTAGCTCTTTCTCATCAA+TGG 0.358749 6_4:-19213409 None:intergenic
GAGGTGTATATTAGAATTAG+TGG 0.358819 6_4:+19213210 Msa0980680:five_prime_UTR
TCAAATACACCAATTTCAAA+TGG 0.374400 6_4:-19212986 None:intergenic
GCTCCATTTAGGGAAATAAT+AGG 0.382373 6_4:-19214286 None:intergenic
ATGGAGCATCTCTTCCTGTT+TGG 0.386995 6_4:+19214302 Msa0980680:CDS
GTTGCGTCATTGTTGCTAAA+GGG 0.417737 6_4:-19213383 None:intergenic
CATTGATGAGAAAGAGCTAA+TGG 0.423043 6_4:+19213410 Msa0980680:CDS
CTTATATGTTATATTCAAGT+TGG 0.430449 6_4:-19212624 None:intergenic
CAAATACACCAATTTCAAAT+GGG 0.437469 6_4:-19212985 None:intergenic
AGTTGCGTCATTGTTGCTAA+AGG 0.438954 6_4:-19213384 None:intergenic
ATGGAGAAGTTTATCAATGA+AGG 0.453704 6_4:+19213429 Msa0980680:CDS
TTCTACCAGAGTCAGTGAAA+TGG 0.475599 6_4:-19212894 None:intergenic
TTTCCATATCATCAAAACTA+AGG 0.483553 6_4:+19212718 Msa0980680:five_prime_UTR
GAAAGTGTGCATATGTTCTA+TGG 0.487435 6_4:+19212468 Msa0980680:five_prime_UTR
AAAATTGATCTTTCAAACAA+TGG 0.489807 6_4:-19213319 None:intergenic
TATGATTACCTTGATTATGT+AGG 0.491010 6_4:-19214334 None:intergenic
GTAGGAGTTGATCTCCAAAC+AGG 0.497937 6_4:-19214316 None:intergenic
CATTACCATTTCACTGACTC+TGG 0.500609 6_4:+19212889 Msa0980680:five_prime_UTR
AGAATGACAAGGAGAGAAGA+TGG 0.502575 6_4:+19214261 Msa0980680:CDS
CACTATAATCGGAGTACTGA+AGG 0.519436 6_4:-19212840 None:intergenic
TGTGCATATGTTCTATGGCT+TGG 0.527509 6_4:+19212473 Msa0980680:five_prime_UTR
GATCAACTCCTACATAATCA+AGG 0.538359 6_4:+19214326 Msa0980680:CDS
TTGCGTCATTGTTGCTAAAG+GGG 0.545310 6_4:-19213382 None:intergenic
TTCTTTAATGACAAATCATG+TGG 0.575518 6_4:-19213019 None:intergenic
GTTAAACTTAATCTACACCT+TGG 0.579874 6_4:+19214478 Msa0980680:CDS
TTCAAGATTTATGTAGCATG+AGG 0.590585 6_4:-19212950 None:intergenic
GTACTCCGATTATAGTGTGA+AGG 0.623559 6_4:+19212846 Msa0980680:five_prime_UTR
CAATATAGGAGGTCGTGTCA+AGG 0.639850 6_4:+19214233 Msa0980680:intron
TCAAGGAGAGAAGAATGACA+AGG 0.695837 6_4:+19214250 Msa0980680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTATGCTTTTTTTTTTA+TGG + chr6_4:19212430-19212449 Msa0980680:five_prime_UTR 10.0%
!!! ATTTTTTTTCTTTTTAAATG+TGG + chr6_4:19212505-19212524 Msa0980680:five_prime_UTR 10.0%
!! TATAACTTCTATAATTAAAA+TGG - chr6_4:19213465-19213484 None:intergenic 10.0%
!!! ATTTTAATTATAGAAGTTAT+AGG + chr6_4:19213464-19213483 Msa0980680:intron 10.0%
!!! TATAGATAATATTTCTAATT+AGG + chr6_4:19213708-19213727 Msa0980680:intron 10.0%
!!! AATTTATGCTTTTTTTTTTA+TGG + chr6_4:19212430-19212449 Msa0980680:five_prime_UTR 10.0%
!!! ATTTTTTTTCTTTTTAAATG+TGG + chr6_4:19212505-19212524 Msa0980680:five_prime_UTR 10.0%
!! TATAACTTCTATAATTAAAA+TGG - chr6_4:19213465-19213484 None:intergenic 10.0%
!!! ATTTTAATTATAGAAGTTAT+AGG + chr6_4:19213464-19213483 Msa0980680:intron 10.0%
!!! TATAGATAATATTTCTAATT+AGG + chr6_4:19213708-19213727 Msa0980680:intron 10.0%
!!! GTATTTTATATTGCTTTATA+TGG - chr6_4:19213270-19213289 None:intergenic 15.0%
!! AAAATAAATAGTAGATTGAT+TGG - chr6_4:19213556-19213575 None:intergenic 15.0%
!! ATTCAATAAAATTGAACAAA+AGG - chr6_4:19214384-19214403 None:intergenic 15.0%
!!! GTATTTTATATTGCTTTATA+TGG - chr6_4:19213270-19213289 None:intergenic 15.0%
!! AAAATAAATAGTAGATTGAT+TGG - chr6_4:19213556-19213575 None:intergenic 15.0%
!! ATTCAATAAAATTGAACAAA+AGG - chr6_4:19214384-19214403 None:intergenic 15.0%
!! CTTATATGTTATATTCAAGT+TGG - chr6_4:19212627-19212646 None:intergenic 20.0%
!! AAAATTGATCTTTCAAACAA+TGG - chr6_4:19213322-19213341 None:intergenic 20.0%
!!! TGAAAGATCAATTTTCTAAT+TGG + chr6_4:19213327-19213346 Msa0980680:five_prime_UTR 20.0%
!! GTAGTAAGAATAATGTAATT+TGG + chr6_4:19213639-19213658 Msa0980680:intron 20.0%
!! ACATGATTTCATCAATTAAA+AGG - chr6_4:19213782-19213801 None:intergenic 20.0%
!! TTACTACTAATAAACAACTT+CGG - chr6_4:19213882-19213901 None:intergenic 20.0%
!! GAAAATTGATGTAACAATAT+AGG + chr6_4:19214219-19214238 Msa0980680:intron 20.0%
!! CTTATATGTTATATTCAAGT+TGG - chr6_4:19212627-19212646 None:intergenic 20.0%
!! AAAATTGATCTTTCAAACAA+TGG - chr6_4:19213322-19213341 None:intergenic 20.0%
!!! TGAAAGATCAATTTTCTAAT+TGG + chr6_4:19213327-19213346 Msa0980680:five_prime_UTR 20.0%
!! GTAGTAAGAATAATGTAATT+TGG + chr6_4:19213639-19213658 Msa0980680:intron 20.0%
!! ACATGATTTCATCAATTAAA+AGG - chr6_4:19213782-19213801 None:intergenic 20.0%
!! TTACTACTAATAAACAACTT+CGG - chr6_4:19213882-19213901 None:intergenic 20.0%
!! GAAAATTGATGTAACAATAT+AGG + chr6_4:19214219-19214238 Msa0980680:intron 20.0%
!! TCTTGAATAAAATGTTTTGC+TGG + chr6_4:19212567-19212586 Msa0980680:five_prime_UTR 25.0%
!! TCAACAGTATAAAAAGGTAT+TGG - chr6_4:19212673-19212692 None:intergenic 25.0%
! AACACTTCAACAGTATAAAA+AGG - chr6_4:19212679-19212698 None:intergenic 25.0%
! TTTCCATATCATCAAAACTA+AGG + chr6_4:19212718-19212737 Msa0980680:five_prime_UTR 25.0%
!!! TATCCTTAGTTTTGATGATA+TGG - chr6_4:19212724-19212743 None:intergenic 25.0%
! CAAATACACCAATTTCAAAT+GGG - chr6_4:19212988-19213007 None:intergenic 25.0%
! TCAAATACACCAATTTCAAA+TGG - chr6_4:19212989-19213008 None:intergenic 25.0%
! TTCTTTAATGACAAATCATG+TGG - chr6_4:19213022-19213041 None:intergenic 25.0%
!! TTGCATTTTTCCAAATTCAT+GGG - chr6_4:19213189-19213208 None:intergenic 25.0%
!! TTTGCATTTTTCCAAATTCA+TGG - chr6_4:19213190-19213209 None:intergenic 25.0%
!!! TTATTTTAGTGTTCTTCACA+AGG - chr6_4:19213677-19213696 None:intergenic 25.0%
! AATTGATGTAACAATATAGG+AGG + chr6_4:19214222-19214241 Msa0980680:intron 25.0%
! TATGATTACCTTGATTATGT+AGG - chr6_4:19214337-19214356 None:intergenic 25.0%
! AGGTTAACATATGATATGTT+GGG - chr6_4:19214364-19214383 None:intergenic 25.0%
! AAGGTTAACATATGATATGT+TGG - chr6_4:19214365-19214384 None:intergenic 25.0%
! TTAAGTTTAACAGAGTTCTA+CGG - chr6_4:19214469-19214488 None:intergenic 25.0%
!! TCTTGAATAAAATGTTTTGC+TGG + chr6_4:19212567-19212586 Msa0980680:five_prime_UTR 25.0%
!! TCAACAGTATAAAAAGGTAT+TGG - chr6_4:19212673-19212692 None:intergenic 25.0%
! AACACTTCAACAGTATAAAA+AGG - chr6_4:19212679-19212698 None:intergenic 25.0%
! TTTCCATATCATCAAAACTA+AGG + chr6_4:19212718-19212737 Msa0980680:five_prime_UTR 25.0%
!!! TATCCTTAGTTTTGATGATA+TGG - chr6_4:19212724-19212743 None:intergenic 25.0%
! CAAATACACCAATTTCAAAT+GGG - chr6_4:19212988-19213007 None:intergenic 25.0%
! TCAAATACACCAATTTCAAA+TGG - chr6_4:19212989-19213008 None:intergenic 25.0%
! TTCTTTAATGACAAATCATG+TGG - chr6_4:19213022-19213041 None:intergenic 25.0%
!! TTGCATTTTTCCAAATTCAT+GGG - chr6_4:19213189-19213208 None:intergenic 25.0%
!! TTTGCATTTTTCCAAATTCA+TGG - chr6_4:19213190-19213209 None:intergenic 25.0%
!!! TTATTTTAGTGTTCTTCACA+AGG - chr6_4:19213677-19213696 None:intergenic 25.0%
! AATTGATGTAACAATATAGG+AGG + chr6_4:19214222-19214241 Msa0980680:intron 25.0%
! TATGATTACCTTGATTATGT+AGG - chr6_4:19214337-19214356 None:intergenic 25.0%
! AGGTTAACATATGATATGTT+GGG - chr6_4:19214364-19214383 None:intergenic 25.0%
! AAGGTTAACATATGATATGT+TGG - chr6_4:19214365-19214384 None:intergenic 25.0%
! TTAAGTTTAACAGAGTTCTA+CGG - chr6_4:19214469-19214488 None:intergenic 25.0%
! TGAATAAAATGTTTTGCTGG+CGG + chr6_4:19212570-19212589 Msa0980680:five_prime_UTR 30.0%
CATCAAAACTAAGGATAGTT+TGG + chr6_4:19212727-19212746 Msa0980680:five_prime_UTR 30.0%
TTCAAGATTTATGTAGCATG+AGG - chr6_4:19212953-19212972 None:intergenic 30.0%
! TTTTATTGACCCCATGAATT+TGG + chr6_4:19213176-19213195 Msa0980680:five_prime_UTR 30.0%
! TGCATTTTTCCAAATTCATG+GGG - chr6_4:19213188-19213207 None:intergenic 30.0%
GAATTTGGAAAAATGCAAAG+AGG + chr6_4:19213191-19213210 Msa0980680:five_prime_UTR 30.0%
GAGGTGTATATTAGAATTAG+TGG + chr6_4:19213210-19213229 Msa0980680:five_prime_UTR 30.0%
ATGGAGAAGTTTATCAATGA+AGG + chr6_4:19213429-19213448 Msa0980680:CDS 30.0%
AGTTTATCAATGAAGGTTAG+TGG + chr6_4:19213436-19213455 Msa0980680:intron 30.0%
TATCTATTAGAACTGCTTAG+AGG - chr6_4:19213592-19213611 None:intergenic 30.0%
! CTAAGCAGTTCTAATAGATA+TGG + chr6_4:19213592-19213611 Msa0980680:intron 30.0%
TCCTATGATGAATTCCATAT+GGG - chr6_4:19213752-19213771 None:intergenic 30.0%
ATCCTATGATGAATTCCATA+TGG - chr6_4:19213753-19213772 None:intergenic 30.0%
GATGAAATCATGTTCCAAAT+TGG + chr6_4:19213789-19213808 Msa0980680:intron 30.0%
! AAAATGTGAGCATTTTGCTT+TGG - chr6_4:19214150-19214169 None:intergenic 30.0%
! TTCAGAGAACACTTCTAAAA+AGG - chr6_4:19214187-19214206 None:intergenic 30.0%
TAACATATGATATGTTGGGT+GGG - chr6_4:19214360-19214379 None:intergenic 30.0%
TTAACATATGATATGTTGGG+TGG - chr6_4:19214361-19214380 None:intergenic 30.0%
GTTAAACTTAATCTACACCT+TGG + chr6_4:19214478-19214497 Msa0980680:CDS 30.0%
! TGAATAAAATGTTTTGCTGG+CGG + chr6_4:19212570-19212589 Msa0980680:five_prime_UTR 30.0%
CATCAAAACTAAGGATAGTT+TGG + chr6_4:19212727-19212746 Msa0980680:five_prime_UTR 30.0%
TTCAAGATTTATGTAGCATG+AGG - chr6_4:19212953-19212972 None:intergenic 30.0%
! TTTTATTGACCCCATGAATT+TGG + chr6_4:19213176-19213195 Msa0980680:five_prime_UTR 30.0%
! TGCATTTTTCCAAATTCATG+GGG - chr6_4:19213188-19213207 None:intergenic 30.0%
GAATTTGGAAAAATGCAAAG+AGG + chr6_4:19213191-19213210 Msa0980680:five_prime_UTR 30.0%
GAGGTGTATATTAGAATTAG+TGG + chr6_4:19213210-19213229 Msa0980680:five_prime_UTR 30.0%
ATGGAGAAGTTTATCAATGA+AGG + chr6_4:19213429-19213448 Msa0980680:CDS 30.0%
AGTTTATCAATGAAGGTTAG+TGG + chr6_4:19213436-19213455 Msa0980680:intron 30.0%
TATCTATTAGAACTGCTTAG+AGG - chr6_4:19213592-19213611 None:intergenic 30.0%
! CTAAGCAGTTCTAATAGATA+TGG + chr6_4:19213592-19213611 Msa0980680:intron 30.0%
TCCTATGATGAATTCCATAT+GGG - chr6_4:19213752-19213771 None:intergenic 30.0%
ATCCTATGATGAATTCCATA+TGG - chr6_4:19213753-19213772 None:intergenic 30.0%
GATGAAATCATGTTCCAAAT+TGG + chr6_4:19213789-19213808 Msa0980680:intron 30.0%
! AAAATGTGAGCATTTTGCTT+TGG - chr6_4:19214150-19214169 None:intergenic 30.0%
! TTCAGAGAACACTTCTAAAA+AGG - chr6_4:19214187-19214206 None:intergenic 30.0%
TAACATATGATATGTTGGGT+GGG - chr6_4:19214360-19214379 None:intergenic 30.0%
TTAACATATGATATGTTGGG+TGG - chr6_4:19214361-19214380 None:intergenic 30.0%
GTTAAACTTAATCTACACCT+TGG + chr6_4:19214478-19214497 Msa0980680:CDS 30.0%
! GAAAGTGTGCATATGTTCTA+TGG + chr6_4:19212468-19212487 Msa0980680:five_prime_UTR 35.0%
! GAATAAAATGTTTTGCTGGC+GGG + chr6_4:19212571-19212590 Msa0980680:five_prime_UTR 35.0%
! AATAAAATGTTTTGCTGGCG+GGG + chr6_4:19212572-19212591 Msa0980680:five_prime_UTR 35.0%
CTTTGCCTTCACACTATAAT+CGG - chr6_4:19212854-19212873 None:intergenic 35.0%
! TAGCAGAACCCATTTGAAAT+TGG + chr6_4:19212977-19212996 Msa0980680:five_prime_UTR 35.0%
! CATTAGCTCTTTCTCATCAA+TGG - chr6_4:19213412-19213431 None:intergenic 35.0%
CATTGATGAGAAAGAGCTAA+TGG + chr6_4:19213410-19213429 Msa0980680:CDS 35.0%
TTGGAGAAGCAAAAATCGTT+TGG - chr6_4:19213537-19213556 None:intergenic 35.0%
! AGCAGTTCTAATAGATATGG+TGG + chr6_4:19213595-19213614 Msa0980680:intron 35.0%
TCAAGTAATCATGTCCCATA+TGG + chr6_4:19213735-19213754 Msa0980680:intron 35.0%
TCCCATATGGAATTCATCAT+AGG + chr6_4:19213748-19213767 Msa0980680:intron 35.0%
TAAGGACATGCTAACCAATT+TGG - chr6_4:19213806-19213825 None:intergenic 35.0%
CAGATATAGTCGCTATTGTA+AGG - chr6_4:19213824-19213843 None:intergenic 35.0%
GCACCTATTATTTCCCTAAA+TGG + chr6_4:19214283-19214302 Msa0980680:CDS 35.0%
GCTCCATTTAGGGAAATAAT+AGG - chr6_4:19214289-19214308 None:intergenic 35.0%
GATCAACTCCTACATAATCA+AGG + chr6_4:19214326-19214345 Msa0980680:CDS 35.0%
AGTTCTACGGAATTGGAATT+TGG - chr6_4:19214456-19214475 None:intergenic 35.0%
TTAACAGAGTTCTACGGAAT+TGG - chr6_4:19214463-19214482 None:intergenic 35.0%
! GAAAGTGTGCATATGTTCTA+TGG + chr6_4:19212468-19212487 Msa0980680:five_prime_UTR 35.0%
! GAATAAAATGTTTTGCTGGC+GGG + chr6_4:19212571-19212590 Msa0980680:five_prime_UTR 35.0%
! AATAAAATGTTTTGCTGGCG+GGG + chr6_4:19212572-19212591 Msa0980680:five_prime_UTR 35.0%
CTTTGCCTTCACACTATAAT+CGG - chr6_4:19212854-19212873 None:intergenic 35.0%
! TAGCAGAACCCATTTGAAAT+TGG + chr6_4:19212977-19212996 Msa0980680:five_prime_UTR 35.0%
! CATTAGCTCTTTCTCATCAA+TGG - chr6_4:19213412-19213431 None:intergenic 35.0%
CATTGATGAGAAAGAGCTAA+TGG + chr6_4:19213410-19213429 Msa0980680:CDS 35.0%
TTGGAGAAGCAAAAATCGTT+TGG - chr6_4:19213537-19213556 None:intergenic 35.0%
! AGCAGTTCTAATAGATATGG+TGG + chr6_4:19213595-19213614 Msa0980680:intron 35.0%
TCAAGTAATCATGTCCCATA+TGG + chr6_4:19213735-19213754 Msa0980680:intron 35.0%
TCCCATATGGAATTCATCAT+AGG + chr6_4:19213748-19213767 Msa0980680:intron 35.0%
TAAGGACATGCTAACCAATT+TGG - chr6_4:19213806-19213825 None:intergenic 35.0%
CAGATATAGTCGCTATTGTA+AGG - chr6_4:19213824-19213843 None:intergenic 35.0%
GCACCTATTATTTCCCTAAA+TGG + chr6_4:19214283-19214302 Msa0980680:CDS 35.0%
GCTCCATTTAGGGAAATAAT+AGG - chr6_4:19214289-19214308 None:intergenic 35.0%
GATCAACTCCTACATAATCA+AGG + chr6_4:19214326-19214345 Msa0980680:CDS 35.0%
AGTTCTACGGAATTGGAATT+TGG - chr6_4:19214456-19214475 None:intergenic 35.0%
TTAACAGAGTTCTACGGAAT+TGG - chr6_4:19214463-19214482 None:intergenic 35.0%
TGTGCATATGTTCTATGGCT+TGG + chr6_4:19212473-19212492 Msa0980680:five_prime_UTR 40.0%
!! CACTATAATCGGAGTACTGA+AGG - chr6_4:19212843-19212862 None:intergenic 40.0%
GTACTCCGATTATAGTGTGA+AGG + chr6_4:19212846-19212865 Msa0980680:five_prime_UTR 40.0%
!! CATTACCATTTCACTGACTC+TGG + chr6_4:19212889-19212908 Msa0980680:five_prime_UTR 40.0%
TTCTACCAGAGTCAGTGAAA+TGG - chr6_4:19212897-19212916 None:intergenic 40.0%
TTGCGTCATTGTTGCTAAAG+GGG - chr6_4:19213385-19213404 None:intergenic 40.0%
GTTGCGTCATTGTTGCTAAA+GGG - chr6_4:19213386-19213405 None:intergenic 40.0%
AGTTGCGTCATTGTTGCTAA+AGG - chr6_4:19213387-19213406 None:intergenic 40.0%
TCAAGGAGAGAAGAATGACA+AGG + chr6_4:19214250-19214269 Msa0980680:CDS 40.0%
AGAATGACAAGGAGAGAAGA+TGG + chr6_4:19214261-19214280 Msa0980680:CDS 40.0%
AGGAAGAGATGCTCCATTTA+GGG - chr6_4:19214299-19214318 None:intergenic 40.0%
TGTGCATATGTTCTATGGCT+TGG + chr6_4:19212473-19212492 Msa0980680:five_prime_UTR 40.0%
!! CACTATAATCGGAGTACTGA+AGG - chr6_4:19212843-19212862 None:intergenic 40.0%
GTACTCCGATTATAGTGTGA+AGG + chr6_4:19212846-19212865 Msa0980680:five_prime_UTR 40.0%
!! CATTACCATTTCACTGACTC+TGG + chr6_4:19212889-19212908 Msa0980680:five_prime_UTR 40.0%
TTCTACCAGAGTCAGTGAAA+TGG - chr6_4:19212897-19212916 None:intergenic 40.0%
TTGCGTCATTGTTGCTAAAG+GGG - chr6_4:19213385-19213404 None:intergenic 40.0%
GTTGCGTCATTGTTGCTAAA+GGG - chr6_4:19213386-19213405 None:intergenic 40.0%
AGTTGCGTCATTGTTGCTAA+AGG - chr6_4:19213387-19213406 None:intergenic 40.0%
TCAAGGAGAGAAGAATGACA+AGG + chr6_4:19214250-19214269 Msa0980680:CDS 40.0%
AGAATGACAAGGAGAGAAGA+TGG + chr6_4:19214261-19214280 Msa0980680:CDS 40.0%
AGGAAGAGATGCTCCATTTA+GGG - chr6_4:19214299-19214318 None:intergenic 40.0%
CAATATAGGAGGTCGTGTCA+AGG + chr6_4:19214233-19214252 Msa0980680:intron 45.0%
CAGGAAGAGATGCTCCATTT+AGG - chr6_4:19214300-19214319 None:intergenic 45.0%
! ATGGAGCATCTCTTCCTGTT+TGG + chr6_4:19214302-19214321 Msa0980680:CDS 45.0%
! GTAGGAGTTGATCTCCAAAC+AGG - chr6_4:19214319-19214338 None:intergenic 45.0%
CAATATAGGAGGTCGTGTCA+AGG + chr6_4:19214233-19214252 Msa0980680:intron 45.0%
CAGGAAGAGATGCTCCATTT+AGG - chr6_4:19214300-19214319 None:intergenic 45.0%
! ATGGAGCATCTCTTCCTGTT+TGG + chr6_4:19214302-19214321 Msa0980680:CDS 45.0%
! GTAGGAGTTGATCTCCAAAC+AGG - chr6_4:19214319-19214338 None:intergenic 45.0%
! TTGCTGGCGGGGTTGCTTTA+AGG + chr6_4:19212583-19212602 Msa0980680:five_prime_UTR 55.0%
! TTGCTGGCGGGGTTGCTTTA+AGG + chr6_4:19212583-19212602 Msa0980680:five_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 19212428 19214513 19212428 ID=Msa0980680;Name=Msa0980680
chr6_4 mRNA 19212428 19214513 19212428 ID=Msa0980680-mRNA-1;Parent=Msa0980680;Name=Msa0980680-mRNA-1;_AED=0.26;_eAED=0.26;_QI=944|0.5|0.66|0.66|0|0.33|3|0|75
chr6_4 exon 19212428 19213450 19212428 ID=Msa0980680-mRNA-1:exon:5949;Parent=Msa0980680-mRNA-1
chr6_4 exon 19214241 19214347 19214241 ID=Msa0980680-mRNA-1:exon:5950;Parent=Msa0980680-mRNA-1
chr6_4 exon 19214472 19214513 19214472 ID=Msa0980680-mRNA-1:exon:5951;Parent=Msa0980680-mRNA-1
chr6_4 five_prime_UTR 19212428 19213371 19212428 ID=Msa0980680-mRNA-1:five_prime_utr;Parent=Msa0980680-mRNA-1
chr6_4 CDS 19213372 19213450 19213372 ID=Msa0980680-mRNA-1:cds;Parent=Msa0980680-mRNA-1
chr6_4 CDS 19214241 19214347 19214241 ID=Msa0980680-mRNA-1:cds;Parent=Msa0980680-mRNA-1
chr6_4 CDS 19214472 19214513 19214472 ID=Msa0980680-mRNA-1:cds;Parent=Msa0980680-mRNA-1
Gene Sequence

>Msa0980680

ATGTCAAATACCCCTTTAGCAACAATGACGCAACTAACCATTGATGAGAAAGAGCTAATGGAGAAGTTTATCAATGAAGGAGGTCGTGTCAAGGAGAGAAGAATGACAAGGAGAGAAGATGGCACCTATTATTTCCCTAAATGGAGCATCTCTTCCTGTTTGGAGATCAACTCCTACATAATCAAGAACTCTGTTAAACTTAATCTACACCTTGGACAAATTGCATAA
Protein sequence

>Msa0980680

MSNTPLATMTQLTIDEKELMEKFINEGGRVKERRMTRREDGTYYFPKWSISSCLEINSYIIKNSVKLNLHLGQIA*