AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0981020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0981020 MtrunA17_Chr6g0461601 97.314 484 13 0 1 484 1 484 0.0 973
Msa0981020 MtrunA17_Chr7g0253091 76.349 482 111 3 1 482 1 479 0.0 748
Msa0981020 MtrunA17_Chr4g0062771 58.405 464 186 5 10 472 18 475 0.0 541
Msa0981020 MtrunA17_Chr1g0148391 56.276 478 197 6 6 478 6 476 0.0 524
Msa0981020 MtrunA17_Chr2g0306311 51.697 501 208 7 10 483 18 511 1.29e-175 503
Msa0981020 MtrunA17_Chr3g0138741 56.044 455 192 5 10 462 15 463 3.72e-174 498
Msa0981020 MtrunA17_Chr8g0383311 55.046 436 190 4 23 457 40 470 2.64e-171 491
Msa0981020 MtrunA17_Chr4g0069801 52.318 453 200 7 25 471 27 469 3.72e-160 462
Msa0981020 MtrunA17_Chr4g0076581 49.478 479 228 6 10 483 17 486 4.16e-157 455
Msa0981020 MtrunA17_Chr3g0129341 52.252 444 204 6 23 465 31 467 1.19e-153 446
Msa0981020 MtrunA17_Chr2g0290821 50.805 435 203 6 26 457 28 454 2.01e-152 443
Msa0981020 MtrunA17_Chr3g0105981 45.913 416 214 8 24 438 25 430 2.32e-114 343
Msa0981020 MtrunA17_Chr3g0144531 33.787 441 265 16 26 453 28 454 9.11e-62 208
Msa0981020 MtrunA17_Chr4g0034901 33.262 466 275 18 9 453 9 459 1.10e-61 208
Msa0981020 MtrunA17_Chr1g0161011 32.609 460 272 17 10 453 8 445 8.65e-61 207
Msa0981020 MtrunA17_Chr8g0389741 30.455 440 281 14 26 454 28 453 5.21e-58 199
Msa0981020 MtrunA17_Chr7g0223961 33.259 448 259 18 23 451 20 446 1.05e-57 197
Msa0981020 MtrunA17_Chr6g0487261 29.701 468 290 17 9 454 5 455 1.73e-55 193
Msa0981020 MtrunA17_Chr5g0439251 31.957 460 251 17 26 447 25 460 5.22e-55 191
Msa0981020 MtrunA17_Chr1g0177171 30.426 470 268 18 26 453 38 490 4.27e-53 186
Msa0981020 MtrunA17_Chr4g0046311 31.057 454 276 19 26 457 23 461 8.25e-53 185
Msa0981020 MtrunA17_Chr4g0038981 32.153 339 208 9 10 344 4 324 7.08e-51 176
Msa0981020 MtrunA17_Chr3g0110371 29.694 458 288 18 10 453 8 445 7.78e-51 180
Msa0981020 MtrunA17_Chr4g0039011 33.742 326 194 10 26 349 42 347 1.03e-50 176
Msa0981020 MtrunA17_Chr1g0146831 34.615 364 206 15 11 357 16 364 1.46e-50 176
Msa0981020 MtrunA17_Chr4g0003281 34.203 345 197 13 13 340 20 351 4.78e-50 176
Msa0981020 MtrunA17_Chr8g0339191 29.705 441 284 15 27 453 30 458 4.92e-50 177
Msa0981020 MtrunA17_Chr4g0038941 33.636 330 196 9 20 346 46 355 5.73e-50 174
Msa0981020 MtrunA17_Chr4g0038961 32.153 339 208 9 10 344 4 324 7.06e-50 173
Msa0981020 MtrunA17_Chr7g0250301 33.538 325 196 11 26 346 8 316 3.15e-49 171
Msa0981020 MtrunA17_Chr4g0039071 32.722 327 202 9 24 346 23 335 6.00e-49 172
Msa0981020 MtrunA17_Chr5g0434591 29.464 448 270 16 26 453 23 444 9.54e-49 174
Msa0981020 MtrunA17_Chr4g0075031 30.700 443 271 17 26 453 45 466 1.86e-48 174
Msa0981020 MtrunA17_Chr1g0189111 33.133 332 197 12 23 344 28 344 2.17e-48 172
Msa0981020 MtrunA17_Chr4g0043851 32.558 344 207 11 8 339 11 341 2.99e-48 171
Msa0981020 MtrunA17_Chr4g0064021 33.025 324 194 11 27 340 27 337 3.04e-48 170
Msa0981020 MtrunA17_Chr4g0038971 31.692 325 203 8 23 346 20 326 3.05e-48 169
Msa0981020 MtrunA17_Chr4g0043511 29.540 457 278 15 26 453 62 503 4.59e-48 174
Msa0981020 MtrunA17_Chr3g0119541 36.061 330 180 13 26 341 26 338 6.81e-48 169
Msa0981020 MtrunA17_Chr8g0378621 28.846 468 293 19 3 453 4 448 9.87e-48 172
Msa0981020 MtrunA17_Chr2g0294591 32.609 322 203 6 26 345 30 339 1.84e-47 167
Msa0981020 MtrunA17_Chr2g0294601 31.988 322 205 6 26 345 59 368 3.66e-47 167
Msa0981020 MtrunA17_Chr3g0121271 29.067 461 292 17 26 472 26 465 5.85e-46 167
Msa0981020 MtrunA17_Chr4g0039021 33.882 304 180 10 46 344 1 288 1.52e-44 158
Msa0981020 MtrunA17_Chr2g0321511 29.805 359 224 12 8 354 9 351 1.58e-43 158
Msa0981020 MtrunA17_Chr4g0026321 31.965 341 206 13 16 344 17 343 2.97e-43 157
Msa0981020 MtrunA17_Chr7g0234821 27.640 445 291 17 27 453 27 458 3.32e-43 159
Msa0981020 MtrunA17_Chr2g0285901 33.333 324 189 12 27 339 25 332 4.32e-42 154
Msa0981020 MtrunA17_Chr1g0174261 32.653 294 180 9 55 344 1 280 2.53e-41 150
Msa0981020 MtrunA17_Chr1g0155001 31.157 337 210 11 26 352 24 348 3.23e-38 144
Msa0981020 MtrunA17_Chr7g0250321 32.852 277 170 9 24 297 26 289 2.54e-36 135
Msa0981020 MtrunA17_Chr5g0436361 29.073 313 192 14 42 344 56 348 3.64e-22 98.2
Msa0981020 MtrunA17_Chr2g0294581 34.074 135 85 2 26 159 76 207 7.87e-16 77.8
Msa0981020 MtrunA17_Chr2g0294581 38.298 94 52 3 251 343 190 278 4.99e-11 63.5
Msa0981020 MtrunA17_Chr4g0018771 33.333 93 59 2 252 344 84 173 5.34e-12 65.5
Msa0981020 MtrunA17_Chr4g0018861 28.571 133 72 3 234 346 44 173 1.70e-11 63.9
Msa0981020 MtrunA17_Chr8g0378401 45.000 80 41 3 371 448 32 110 9.40e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0981020 AT1G64760.1 73.793 435 110 4 23 457 22 452 0.0 648
Msa0981020 AT1G64760.2 73.793 435 110 4 23 457 22 452 0.0 648
Msa0981020 AT2G19440.2 71.492 449 122 5 9 457 6 448 0.0 645
Msa0981020 AT2G19440.1 71.492 449 122 5 9 457 6 448 0.0 645
Msa0981020 AT5G18220.1 66.094 466 145 7 23 483 27 484 0.0 613
Msa0981020 AT3G04010.1 66.593 455 144 6 12 462 16 466 0.0 612
Msa0981020 AT4G31140.1 53.374 489 212 7 3 483 4 484 0.0 516
Msa0981020 AT3G24330.1 54.077 466 200 7 26 481 39 500 6.87e-175 501
Msa0981020 AT5G58090.1 55.963 436 184 5 26 459 22 451 7.14e-174 498
Msa0981020 AT5G64790.1 54.545 451 199 4 12 461 14 459 6.23e-168 483
Msa0981020 AT5G20870.1 53.187 455 195 7 16 460 20 466 5.64e-157 455
Msa0981020 AT4G17180.1 49.206 441 208 7 26 464 25 451 4.89e-145 424
Msa0981020 AT5G58480.1 44.196 448 239 5 23 469 23 460 1.62e-122 366
Msa0981020 AT5G55180.1 32.743 452 258 16 26 453 27 456 3.58e-63 212
Msa0981020 AT3G55430.1 33.775 453 259 20 18 453 21 449 4.95e-63 212
Msa0981020 AT5G55180.2 32.644 435 261 15 26 443 27 446 2.65e-62 210
Msa0981020 AT2G05790.1 32.972 461 259 19 26 453 25 468 8.27e-62 209
Msa0981020 AT2G16230.1 34.004 447 250 18 26 451 25 447 2.82e-60 206
Msa0981020 AT4G26830.1 31.064 470 279 17 6 453 5 451 1.72e-58 200
Msa0981020 AT4G26830.2 31.778 450 262 17 26 453 29 455 1.31e-57 197
Msa0981020 AT2G39640.1 32.755 461 266 20 11 455 12 444 5.65e-57 198
Msa0981020 AT2G01630.1 31.448 442 269 18 26 453 23 444 9.25e-57 196
Msa0981020 AT1G66250.1 31.519 441 269 16 26 453 32 452 9.43e-56 194
Msa0981020 AT2G01630.3 31.193 436 266 18 26 447 23 438 1.58e-55 191
Msa0981020 AT5G24318.1 29.274 468 281 20 12 453 12 455 2.64e-55 191
Msa0981020 AT5G24318.2 29.476 458 273 20 12 443 12 445 1.35e-54 189
Msa0981020 AT4G14080.1 30.568 458 288 17 13 453 29 473 5.08e-53 186
Msa0981020 AT4G16260.1 34.043 329 199 9 24 349 22 335 6.29e-53 182
Msa0981020 AT4G34480.1 31.973 441 268 16 26 453 26 447 2.57e-52 184
Msa0981020 AT5G56590.1 28.412 447 292 13 22 454 22 454 6.16e-52 184
Msa0981020 AT3G07320.1 30.926 443 277 18 27 453 26 455 6.20e-52 182
Msa0981020 AT4G16260.2 34.286 315 190 8 37 349 7 306 6.45e-52 178
Msa0981020 AT3G23770.1 30.769 455 284 17 18 453 29 471 1.05e-51 182
Msa0981020 AT3G57270.1 35.093 322 193 9 26 344 27 335 2.17e-51 178
Msa0981020 AT3G57260.1 32.743 339 205 11 10 344 15 334 5.77e-51 177
Msa0981020 AT3G57260.2 32.743 339 205 11 10 344 31 350 7.80e-51 177
Msa0981020 AT1G11820.2 30.248 443 273 17 26 453 44 465 1.75e-50 180
Msa0981020 AT3G13560.4 29.935 461 278 15 26 467 26 460 6.07e-50 178
Msa0981020 AT3G13560.1 29.935 461 278 15 26 467 26 460 6.07e-50 178
Msa0981020 AT3G13560.2 29.935 461 278 15 26 467 26 460 6.07e-50 178
Msa0981020 AT3G13560.5 29.935 461 278 15 26 467 26 460 6.07e-50 178
Msa0981020 AT3G13560.3 29.935 461 278 15 26 467 26 460 6.07e-50 178
Msa0981020 AT1G11820.3 30.206 437 269 17 26 447 44 459 2.04e-49 176
Msa0981020 AT3G15800.1 33.234 334 199 12 18 340 35 355 1.01e-48 172
Msa0981020 AT4G18340.2 31.653 357 214 12 1 340 1 344 3.96e-48 170
Msa0981020 AT4G18340.1 31.897 348 210 11 7 340 10 344 1.18e-47 169
Msa0981020 AT1G30080.2 32.544 338 202 11 13 339 23 345 3.28e-47 168
Msa0981020 AT1G30080.1 32.544 338 202 11 13 339 23 345 5.58e-47 168
Msa0981020 AT1G32860.1 32.822 326 195 10 26 340 29 341 5.10e-46 166
Msa0981020 AT4G29360.1 26.195 481 308 15 9 455 10 477 1.37e-45 167
Msa0981020 AT2G01630.2 31.940 335 205 12 26 349 23 345 4.45e-45 162
Msa0981020 AT2G26600.1 29.972 357 225 12 22 366 30 373 6.30e-45 162
Msa0981020 AT3G57240.1 31.692 325 199 9 23 344 32 336 9.47e-45 160
Msa0981020 AT4G29360.2 26.069 491 310 16 5 455 10 487 6.47e-44 162
Msa0981020 AT5G42720.1 34.462 325 189 13 26 339 26 337 2.98e-43 159
Msa0981020 AT4G34480.2 36.111 324 184 13 26 339 26 336 5.34e-43 156
Msa0981020 AT5G24318.4 30.636 346 212 14 12 344 12 342 6.16e-43 156
Msa0981020 AT5G42100.2 33.923 339 197 13 23 348 24 348 8.82e-42 154
Msa0981020 AT5G42100.1 33.923 339 197 13 23 348 24 348 1.54e-41 154
Msa0981020 AT3G61810.1 28.994 338 206 12 26 349 55 372 8.75e-41 150
Msa0981020 AT2G27500.2 30.211 331 208 11 24 344 27 344 3.17e-40 149
Msa0981020 AT2G27500.1 30.211 331 208 11 24 344 27 344 7.22e-40 148
Msa0981020 AT4G34480.3 32.571 350 208 14 116 453 3 336 2.91e-38 144
Msa0981020 AT1G77780.1 28.923 325 209 9 27 346 25 332 3.53e-38 143
Msa0981020 AT1G11820.1 29.532 342 220 10 26 357 44 374 6.93e-38 143
Msa0981020 AT5G20390.1 30.488 328 204 11 26 346 33 343 4.76e-36 137
Msa0981020 AT1G77790.1 29.143 350 218 12 8 344 7 339 8.74e-36 136
Msa0981020 AT5G24318.3 29.394 330 204 14 139 453 1 316 5.70e-35 133
Msa0981020 AT5G20330.1 30.183 328 205 11 26 346 34 344 1.20e-33 130
Msa0981020 AT5G20340.1 29.653 317 200 10 26 336 43 342 1.47e-33 130
Msa0981020 AT1G33220.1 30.033 303 192 9 26 322 34 322 5.35e-33 128
Msa0981020 AT2G26600.2 28.920 287 180 11 91 366 6 279 1.14e-30 120
Msa0981020 AT3G46570.1 26.611 357 231 13 1 344 2 340 1.99e-30 121
Msa0981020 AT2G27500.3 30.108 279 172 11 76 344 1 266 3.59e-30 120
Msa0981020 AT3G55780.1 26.205 332 219 9 26 344 31 349 1.94e-29 119
Msa0981020 AT5G20560.1 27.129 317 209 10 26 336 34 334 3.99e-28 114
Msa0981020 AT2G43670.1 39.024 82 48 2 371 451 35 115 1.90e-11 61.6

Find 104 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACATTGAAGTTTATTTATT+TGG 0.091611 6_4:-19706489 Msa0981020:CDS
TTCATAAACATCAGCATTTA+AGG 0.121944 6_4:+19706932 None:intergenic
GAAACATGCATGGGAAATTT+AGG 0.155283 6_4:+19706387 None:intergenic
TGCAAGGTTGGTTAAATTAT+TGG 0.155408 6_4:+19706278 None:intergenic
CACCTAATCCAACTTGATTT+AGG 0.179677 6_4:+19706976 None:intergenic
TCTTCTTGTACACATGTTAT+AGG 0.180643 6_4:-19705948 Msa0981020:three_prime_UTR
GGATTAAACATGCACCATTT+TGG 0.186507 6_4:+19706306 None:intergenic
TATCTTTGAGTGAAAATTAA+TGG 0.197627 6_4:-19708997 Msa0981020:exon
ATTTCAGCTAATTGATTATT+AGG 0.201538 6_4:+19708741 None:intergenic
ATGGATCATTAGATGGAATT+TGG 0.214314 6_4:+19709585 None:intergenic
TCAATGTAACCTTGTCTTCT+TGG 0.216980 6_4:+19706504 None:intergenic
TGGATCATCTTGCAACAATT+TGG 0.228531 6_4:-19706205 Msa0981020:CDS
ACATGGTCAAAATAAATTAT+TGG 0.229515 6_4:-19706358 Msa0981020:CDS
AACATTTGTACAACTGTATC+TGG 0.259099 6_4:+19708861 None:intergenic
AAATTCCATTGTCATTTATA+GGG 0.260086 6_4:+19706718 None:intergenic
AAAATTCCATTGTCATTTAT+AGG 0.268255 6_4:+19706717 None:intergenic
CTATTCTATGTTTGATTGAT+TGG 0.274287 6_4:+19709394 None:intergenic
ATGTATGTGGTGTGAAAATA+AGG 0.275216 6_4:+19709520 None:intergenic
GAAGTATGATAATCTAATTA+AGG 0.279119 6_4:+19709473 None:intergenic
ATCTTTGAGTGAAAATTAAT+GGG 0.281401 6_4:-19708996 Msa0981020:exon
AGCACTTCTATATTTGTATA+TGG 0.290955 6_4:+19709073 None:intergenic
GTAAGAAGAAGCTAGGAAAA+TGG 0.309827 6_4:+19709265 None:intergenic
TTCCATCAGCTGGAATATTT+AGG 0.313567 6_4:-19706887 Msa0981020:CDS
ACCTAATCCAACTTGATTTA+GGG 0.313740 6_4:+19706977 None:intergenic
CCTAGGCTTGCATCAAATAA+AGG 0.313854 6_4:-19706531 Msa0981020:CDS
GGAAGGACTTGGTGTGAATT+GGG 0.317702 6_4:-19708910 Msa0981020:CDS
TGCAACAATTTGGATGCTAA+TGG 0.318329 6_4:-19706195 Msa0981020:CDS
TGGAAGGACTTGGTGTGAAT+TGG 0.327363 6_4:-19708911 Msa0981020:CDS
TTGGTTAAATTATTGGCATT+TGG 0.329109 6_4:+19706285 None:intergenic
GATTGCACCCCACTTGGATA+TGG 0.332800 6_4:-19706225 Msa0981020:CDS
GCATTGAAATCAATTGGATT+TGG 0.334676 6_4:-19706651 Msa0981020:CDS
TGGATTAGGTGACTCAATTA+AGG 0.336092 6_4:-19706964 Msa0981020:CDS
ACCTTTGATTCTATTGTGTT+AGG 0.337915 6_4:-19705977 Msa0981020:exon
CTCAAAATTTCCTGGCTCAA+TGG 0.343066 6_4:+19706443 None:intergenic
GTCTCAGCATTGAAATCAAT+TGG 0.343874 6_4:-19706657 Msa0981020:CDS
GTACAAATGTTGAAAGATAA+TGG 0.351290 6_4:-19708849 Msa0981020:CDS
TGCATTATCTTGATCCAAAA+TGG 0.352525 6_4:-19706320 Msa0981020:CDS
CAATGTCTCTCAAAATTTCC+TGG 0.367879 6_4:+19706435 None:intergenic
TATTCTATGTTTGATTGATT+GGG 0.368789 6_4:+19709395 None:intergenic
GGAACAGCATTGTTTGAATC+TGG 0.376124 6_4:+19706906 None:intergenic
ACTCGTTATAACTTCAAATC+TGG 0.376451 6_4:-19708675 Msa0981020:CDS
AAGTATGATAATCTAATTAA+GGG 0.381909 6_4:+19709474 None:intergenic
GTCATAAGTTCACTTAAATC+AGG 0.389740 6_4:+19706864 None:intergenic
ACTAGAAATGGATCATTAGA+TGG 0.397875 6_4:+19709578 None:intergenic
AAGAAGAAGAAAACTAGAAA+TGG 0.410233 6_4:+19709566 None:intergenic
AACAATGCTGTTCCATCAGC+TGG 0.414573 6_4:-19706897 Msa0981020:CDS
AACAAGAAAGCAACAAATGA+AGG 0.414915 6_4:+19706012 None:intergenic
ATTAATATTATCTGCAAGGT+TGG 0.426819 6_4:+19706266 None:intergenic
ATGAGTGCACTTGGTGGGTC+AGG 0.429886 6_4:-19708786 Msa0981020:CDS
GCCCTAAATCAAGTTGGATT+AGG 0.441972 6_4:-19706978 Msa0981020:CDS
ATAGGAGTGAAATTTATTAG+TGG 0.469236 6_4:+19709197 None:intergenic
TTATGATCGTGCTTTGCAAT+GGG 0.469916 6_4:-19708712 Msa0981020:CDS
AATGCATTAAGATTCTATAA+TGG 0.474784 6_4:-19706561 Msa0981020:CDS
AACAAAGAAGAGGATGAAGA+AGG 0.489426 6_4:+19706036 None:intergenic
TGAAAATGAGATCTTGTACT+TGG 0.497057 6_4:-19705897 Msa0981020:three_prime_UTR
GGCCTAAATATTCCAGCTGA+TGG 0.497077 6_4:+19706885 None:intergenic
CCTTTATTTGATGCAAGCCT+AGG 0.497501 6_4:+19706531 None:intergenic
TCATAAACATCAGCATTTAA+GGG 0.503342 6_4:+19706933 None:intergenic
TATTTAGAGATGATTAAGAA+AGG 0.506239 6_4:+19709609 None:intergenic
ACAACTGTATCTGGTTTCAA+TGG 0.506632 6_4:+19708870 None:intergenic
CAAAATGCCCTAAATCAAGT+TGG 0.507329 6_4:-19706984 Msa0981020:CDS
TTTGCTGATTGCACCCCACT+TGG 0.508951 6_4:-19706231 Msa0981020:CDS
ACAAAGAAGAGGATGAAGAA+GGG 0.509172 6_4:+19706037 None:intergenic
TGATACAGGTATGTAGCAGT+TGG 0.511141 6_4:-19707077 Msa0981020:intron
AAGAAGCTAGGAAAATGGAG+TGG 0.511243 6_4:+19709270 None:intergenic
GGGTAAATGTTGACCGTGAA+TGG 0.513460 6_4:+19706807 None:intergenic
ATTATGATCGTGCTTTGCAA+TGG 0.517702 6_4:-19708713 Msa0981020:CDS
GGTCAAAATAAATTATTGGT+TGG 0.518395 6_4:-19706354 Msa0981020:CDS
TGAACAAGTAAGAAGAAGCT+AGG 0.523091 6_4:+19709258 None:intergenic
CATAATTAATATTATCTGCA+AGG 0.534309 6_4:+19706262 None:intergenic
TGCACTCATAGTTTCTTCAT+CGG 0.540474 6_4:+19708800 None:intergenic
TGAATAATAAAATCACAAGT+AGG 0.543706 6_4:+19706066 None:intergenic
AATGTTCATTAGGGCAGGGA+AGG 0.547103 6_4:+19707007 None:intergenic
GATGCAAAATCCATTGAGCC+AGG 0.551835 6_4:-19706453 Msa0981020:CDS
AGAAGCTAGGAAAATGGAGT+GGG 0.553250 6_4:+19709271 None:intergenic
TGGGTCAGGTATTGAAGTAA+TGG 0.555721 6_4:-19708772 Msa0981020:CDS
ACCTAACACAATAGAATCAA+AGG 0.557731 6_4:+19705976 None:intergenic
CGTTATAACTTCAAATCTGG+TGG 0.565387 6_4:-19708672 Msa0981020:CDS
GAAGTAGGTTGGCCCACAGA+TGG 0.567464 6_4:-19706606 Msa0981020:CDS
ACTTCTATATTTGTATATGG+TGG 0.568585 6_4:+19709076 None:intergenic
ATTCTATGTTTGATTGATTG+GGG 0.569960 6_4:+19709396 None:intergenic
TCTTTGAGTGAAAATTAATG+GGG 0.578888 6_4:-19708995 Msa0981020:exon
AAACTATGAGTGCACTTGGT+GGG 0.583255 6_4:-19708791 Msa0981020:CDS
ACAAGAAAGCAACAAATGAA+GGG 0.589905 6_4:+19706013 None:intergenic
TCAAACCCTATAAATGACAA+TGG 0.600273 6_4:-19706723 Msa0981020:CDS
TGTATGTGGTGTGAAAATAA+GGG 0.608924 6_4:+19709521 None:intergenic
TGAGATCTTGTACTTGGAGA+TGG 0.616005 6_4:-19705891 Msa0981020:three_prime_UTR
GAAGGACTTGGTGTGAATTG+GGG 0.620435 6_4:-19708909 Msa0981020:CDS
AAGATGATCCATATCCAAGT+GGG 0.621191 6_4:+19706217 None:intergenic
AAGTATATGAATTATGTATG+TGG 0.630200 6_4:+19709507 None:intergenic
TCTTGTACTTGGAGATGGTG+TGG 0.634220 6_4:-19705886 Msa0981020:three_prime_UTR
ATGTTGATTTGAAACATGCA+TGG 0.636846 6_4:+19706377 None:intergenic
AAATGAAGGGAACAAAGAAG+AGG 0.637162 6_4:+19706026 None:intergenic
AGTGCAAGATCAGAATGACT+TGG 0.640166 6_4:-19706139 Msa0981020:CDS
TTTGAATGTTCATTAGGGCA+GGG 0.642889 6_4:+19707003 None:intergenic
GAAGAAACTATGAGTGCACT+TGG 0.649749 6_4:-19708795 Msa0981020:CDS
TGTTGATTTGAAACATGCAT+GGG 0.655324 6_4:+19706378 None:intergenic
ATCAGCATTTAAGGGTACTG+TGG 0.661565 6_4:+19706941 None:intergenic
GAAACTATGAGTGCACTTGG+TGG 0.667838 6_4:-19708792 Msa0981020:CDS
AAAGGTACACCAAGAAGACA+AGG 0.670968 6_4:-19706513 Msa0981020:CDS
CAAGATGATCCATATCCAAG+TGG 0.675663 6_4:+19706216 None:intergenic
AGATGATCCATATCCAAGTG+GGG 0.711276 6_4:+19706218 None:intergenic
ATGTGGTGTGAAAATAAGGG+AGG 0.718069 6_4:+19709524 None:intergenic
ATGCATGTTTCAAATCAACA+TGG 0.723172 6_4:-19706375 Msa0981020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTTAAAAATATATT+TGG + chr6_4:19706437-19706456 None:intergenic 0.0%
!! AAAATATATACTAGTTAATA+TGG - chr6_4:19707132-19707151 Msa0981020:intron 10.0%
!!! TGCATTTTTTTTTTTTTATA+TGG - chr6_4:19707593-19707612 Msa0981020:intron 10.0%
!! AAGTATGATAATCTAATTAA+GGG + chr6_4:19706007-19706026 None:intergenic 15.0%
!!! GTTTTTATTTTTAATTGTTG+TGG - chr6_4:19706886-19706905 Msa0981020:CDS 15.0%
!!! CTTTTTTTAATGTCAATTAT+TGG - chr6_4:19707209-19707228 Msa0981020:intron 15.0%
!! TTTGAACAATAAACTAAAAT+TGG + chr6_4:19707417-19707436 None:intergenic 15.0%
!!! TTTTTTTTTTTTTATATGGC+TGG - chr6_4:19707597-19707616 Msa0981020:intron 15.0%
!!! GACTAAAATTCATATTTTTA+AGG - chr6_4:19707942-19707961 Msa0981020:intron 15.0%
!!! ATATTTTTAAGGTGAATAAA+CGG - chr6_4:19707953-19707972 Msa0981020:intron 15.0%
!!! TCTTTAGCTTAAAAAAAAAA+CGG - chr6_4:19708203-19708222 Msa0981020:intron 15.0%
!! TACATTGAAGTTTATTTATT+TGG - chr6_4:19708989-19709008 Msa0981020:exon 15.0%
!!! ACTTGAATATATTATTTTGA+AGG - chr6_4:19709657-19709676 Msa0981020:five_prime_UTR 15.0%
!!! CTTGAATATATTATTTTGAA+GGG - chr6_4:19709658-19709677 Msa0981020:five_prime_UTR 15.0%
!! TATTTAGAGATGATTAAGAA+AGG + chr6_4:19705872-19705891 None:intergenic 20.0%
!! AAGTATATGAATTATGTATG+TGG + chr6_4:19705974-19705993 None:intergenic 20.0%
!! GAAGTATGATAATCTAATTA+AGG + chr6_4:19706008-19706027 None:intergenic 20.0%
!! TATTCTATGTTTGATTGATT+GGG + chr6_4:19706086-19706105 None:intergenic 20.0%
!! TATCTTTGAGTGAAAATTAA+TGG - chr6_4:19706481-19706500 Msa0981020:CDS 20.0%
!! ATCTTTGAGTGAAAATTAAT+GGG - chr6_4:19706482-19706501 Msa0981020:CDS 20.0%
!! ATTTCAGCTAATTGATTATT+AGG + chr6_4:19706740-19706759 None:intergenic 20.0%
!! CAAAAAATGTCAAAACTATT+AGG - chr6_4:19707001-19707020 Msa0981020:CDS 20.0%
!! TAAAACTGATAAGAAATGAT+CGG - chr6_4:19707280-19707299 Msa0981020:intron 20.0%
!! GAATATGATTCTATAAACTA+AGG - chr6_4:19707325-19707344 Msa0981020:intron 20.0%
!!! TTAACTTCTAGTATGTTTTT+TGG - chr6_4:19707487-19707506 Msa0981020:intron 20.0%
!!! GATTCTTTTTCTTTTTTTCT+TGG - chr6_4:19707723-19707742 Msa0981020:intron 20.0%
!! AAACAAAACTAGATCTTAAT+TGG - chr6_4:19707797-19707816 Msa0981020:intron 20.0%
!!! CATCATATTTTTTTTGTAAC+TGG - chr6_4:19707995-19708014 Msa0981020:intron 20.0%
!!! AGTTTTTGTTAGAATAGTTT+AGG - chr6_4:19708061-19708080 Msa0981020:intron 20.0%
!!! GTTTTTGTTAGAATAGTTTA+GGG - chr6_4:19708062-19708081 Msa0981020:intron 20.0%
!! GTGTTTAGGAAATAAATAAT+TGG + chr6_4:19708350-19708369 None:intergenic 20.0%
!! TATCAATAATAGTGTAAAGA+TGG + chr6_4:19708386-19708405 None:intergenic 20.0%
!! TTGTTGTATGATTTCAAAAA+TGG + chr6_4:19708434-19708453 None:intergenic 20.0%
!!! CATTATTTTTGCTCAAAAAT+TGG + chr6_4:19708649-19708668 None:intergenic 20.0%
!! TTCCATAAAGACTTAAAAAA+GGG + chr6_4:19708694-19708713 None:intergenic 20.0%
!! TTTCCATAAAGACTTAAAAA+AGG + chr6_4:19708695-19708714 None:intergenic 20.0%
!!! AAATTCCATTGTCATTTATA+GGG + chr6_4:19708763-19708782 None:intergenic 20.0%
!!! AAAATTCCATTGTCATTTAT+AGG + chr6_4:19708764-19708783 None:intergenic 20.0%
!! AATGCATTAAGATTCTATAA+TGG - chr6_4:19708917-19708936 Msa0981020:CDS 20.0%
!! ACATGGTCAAAATAAATTAT+TGG - chr6_4:19709120-19709139 Msa0981020:five_prime_UTR 20.0%
!! CATAATTAATATTATCTGCA+AGG + chr6_4:19709219-19709238 None:intergenic 20.0%
!! TGAATAATAAAATCACAAGT+AGG + chr6_4:19709415-19709434 None:intergenic 20.0%
! AAGAAGAAGAAAACTAGAAA+TGG + chr6_4:19705915-19705934 None:intergenic 25.0%
! TATAAAAAGCTAGAACAAGT+TGG + chr6_4:19706041-19706060 None:intergenic 25.0%
! ATTCTATGTTTGATTGATTG+GGG + chr6_4:19706085-19706104 None:intergenic 25.0%
! CTATTCTATGTTTGATTGAT+TGG + chr6_4:19706087-19706106 None:intergenic 25.0%
! ATAGGAGTGAAATTTATTAG+TGG + chr6_4:19706284-19706303 None:intergenic 25.0%
! TTTCCTTACACACTAAAAAT+AGG + chr6_4:19706302-19706321 None:intergenic 25.0%
! ACTTCTATATTTGTATATGG+TGG + chr6_4:19706405-19706424 None:intergenic 25.0%
! AGCACTTCTATATTTGTATA+TGG + chr6_4:19706408-19706427 None:intergenic 25.0%
! TCTTTGAGTGAAAATTAATG+GGG - chr6_4:19706483-19706502 Msa0981020:CDS 25.0%
! GAAAATTAATGGGGAAAAAT+TGG - chr6_4:19706492-19706511 Msa0981020:CDS 25.0%
!!! GAAAAATTGGTTTTCAAGAT+GGG - chr6_4:19706505-19706524 Msa0981020:CDS 25.0%
!!! TGATGATGAGTACAATTTTT+TGG - chr6_4:19706540-19706559 Msa0981020:CDS 25.0%
! GTACAAATGTTGAAAGATAA+TGG - chr6_4:19706629-19706648 Msa0981020:CDS 25.0%
! AAATGTCAAAACTATTAGGT+CGG - chr6_4:19707005-19707024 Msa0981020:CDS 25.0%
! TTAATGTCAATTATTGGACT+AGG - chr6_4:19707215-19707234 Msa0981020:intron 25.0%
!!! AATGATCGGTTGATAATTAT+TGG - chr6_4:19707294-19707313 Msa0981020:intron 25.0%
! AGGAAATCATACATTTCATA+TGG - chr6_4:19707345-19707364 Msa0981020:intron 25.0%
! TTATGTTTGAGTAGTTACTT+TGG - chr6_4:19707565-19707584 Msa0981020:intron 25.0%
! TCGTAAAAAATCAAACCAAA+CGG + chr6_4:19708093-19708112 None:intergenic 25.0%
!! AATAACAAATGAAGAGTACA+GGG + chr6_4:19708172-19708191 None:intergenic 25.0%
!! AAATAACAAATGAAGAGTAC+AGG + chr6_4:19708173-19708192 None:intergenic 25.0%
! TTAAAAAAAAAACGGTGTGT+TGG - chr6_4:19708211-19708230 Msa0981020:intron 25.0%
! TAAAAAAAAAACGGTGTGTT+GGG - chr6_4:19708212-19708231 Msa0981020:intron 25.0%
! ATTACCAACAAAAAATATGC+AGG + chr6_4:19708253-19708272 None:intergenic 25.0%
! TATTTATTTGTGTGTGAATC+AGG - chr6_4:19708274-19708293 Msa0981020:intron 25.0%
! ATTTATTTGTGTGTGAATCA+GGG - chr6_4:19708275-19708294 Msa0981020:intron 25.0%
! GAATGCATAAATATGTGTTT+AGG + chr6_4:19708364-19708383 None:intergenic 25.0%
! CTTTACACTATTATTGATAC+AGG - chr6_4:19708387-19708406 Msa0981020:intron 25.0%
! TCATAAACATCAGCATTTAA+GGG + chr6_4:19708548-19708567 None:intergenic 25.0%
! TTCATAAACATCAGCATTTA+AGG + chr6_4:19708549-19708568 None:intergenic 25.0%
!!! TACCCTTTTTTAAGTCTTTA+TGG - chr6_4:19708689-19708708 Msa0981020:CDS 25.0%
! TCAAAAAATGCAAAGTCAAA+TGG + chr6_4:19708728-19708747 None:intergenic 25.0%
!!! TTTGACTTTGCATTTTTTGA+TGG - chr6_4:19708728-19708747 Msa0981020:CDS 25.0%
! GGTCAAAATAAATTATTGGT+TGG - chr6_4:19709124-19709143 Msa0981020:five_prime_UTR 25.0%
! TTGGTTAAATTATTGGCATT+TGG + chr6_4:19709196-19709215 None:intergenic 25.0%
! ATTAATATTATCTGCAAGGT+TGG + chr6_4:19709215-19709234 None:intergenic 25.0%
ATGGATCATTAGATGGAATT+TGG + chr6_4:19705896-19705915 None:intergenic 30.0%
ACTAGAAATGGATCATTAGA+TGG + chr6_4:19705903-19705922 None:intergenic 30.0%
TGTATGTGGTGTGAAAATAA+GGG + chr6_4:19705960-19705979 None:intergenic 30.0%
ATGTATGTGGTGTGAAAATA+AGG + chr6_4:19705961-19705980 None:intergenic 30.0%
!! AGTCATTTTCTTTCTTCACT+TGG - chr6_4:19706121-19706140 Msa0981020:CDS 30.0%
!! GTCATTTTCTTTCTTCACTT+GGG - chr6_4:19706122-19706141 Msa0981020:CDS 30.0%
! TCATTTTCTTTCTTCACTTG+GGG - chr6_4:19706123-19706142 Msa0981020:CDS 30.0%
!!! ACTCCTATTTTTAGTGTGTA+AGG - chr6_4:19706296-19706315 Msa0981020:CDS 30.0%
!!! GGAAAAATTGGTTTTCAAGA+TGG - chr6_4:19706504-19706523 Msa0981020:CDS 30.0%
!!! GAGTACAATTTTTTGGTATG+TGG - chr6_4:19706547-19706566 Msa0981020:CDS 30.0%
!!! ACAATTTTTTGGTATGTGGA+AGG - chr6_4:19706551-19706570 Msa0981020:CDS 30.0%
AACATTTGTACAACTGTATC+TGG + chr6_4:19706620-19706639 None:intergenic 30.0%
ACTCGTTATAACTTCAAATC+TGG - chr6_4:19706803-19706822 Msa0981020:CDS 30.0%
!!! TGGTTTTATATTTCACTTGC+TGG - chr6_4:19706918-19706937 Msa0981020:CDS 30.0%
! TTTATATTTCACTTGCTGGT+TGG - chr6_4:19706922-19706941 Msa0981020:CDS 30.0%
TTGAAGTTCACACATATAAG+TGG + chr6_4:19707085-19707104 None:intergenic 30.0%
ATGAGTTATTCGATCTTGAT+CGG + chr6_4:19707389-19707408 None:intergenic 30.0%
!! AAATGCTTTTTGTTCTGACA+TGG - chr6_4:19707628-19707647 Msa0981020:intron 30.0%
CTTAGCAAAGTGTACAATTT+CGG - chr6_4:19707694-19707713 Msa0981020:intron 30.0%
CAAAACTAGATCTTAATTGG+TGG - chr6_4:19707800-19707819 Msa0981020:intron 30.0%
AACAAAAACTCACACATCAT+GGG + chr6_4:19708051-19708070 None:intergenic 30.0%
TAACAAAAACTCACACATCA+TGG + chr6_4:19708052-19708071 None:intergenic 30.0%
! GGCATTTTGAATGTTCATTA+GGG + chr6_4:19708483-19708502 None:intergenic 30.0%
ACCTAATCCAACTTGATTTA+GGG + chr6_4:19708504-19708523 None:intergenic 30.0%
GTCATAAGTTCACTTAAATC+AGG + chr6_4:19708617-19708636 None:intergenic 30.0%
CAAAAATAATGCACCATTCA+CGG - chr6_4:19708658-19708677 Msa0981020:intron 30.0%
TCAAACCCTATAAATGACAA+TGG - chr6_4:19708755-19708774 Msa0981020:CDS 30.0%
GCATTGAAATCAATTGGATT+TGG - chr6_4:19708827-19708846 Msa0981020:CDS 30.0%
! GGTAACATTCCAATTTTTGT+TGG - chr6_4:19708848-19708867 Msa0981020:CDS 30.0%
! TCTATAATGGTCTTTTACCT+AGG - chr6_4:19708930-19708949 Msa0981020:CDS 30.0%
!! AGGAAATTTTGAGAGACATT+GGG - chr6_4:19709045-19709064 Msa0981020:five_prime_UTR 30.0%
!!! TGGGGAATTTTTAGTTTTGA+TGG - chr6_4:19709064-19709083 Msa0981020:five_prime_UTR 30.0%
!! TGTTGATTTGAAACATGCAT+GGG + chr6_4:19709103-19709122 None:intergenic 30.0%
!! ATGTTGATTTGAAACATGCA+TGG + chr6_4:19709104-19709123 None:intergenic 30.0%
ATGCATGTTTCAAATCAACA+TGG - chr6_4:19709103-19709122 Msa0981020:five_prime_UTR 30.0%
TGCATTATCTTGATCCAAAA+TGG - chr6_4:19709158-19709177 Msa0981020:five_prime_UTR 30.0%
! TGCAAGGTTGGTTAAATTAT+TGG + chr6_4:19709203-19709222 None:intergenic 30.0%
ACAAGAAAGCAACAAATGAA+GGG + chr6_4:19709468-19709487 None:intergenic 30.0%
AACAAGAAAGCAACAAATGA+AGG + chr6_4:19709469-19709488 None:intergenic 30.0%
ACCTTTGATTCTATTGTGTT+AGG - chr6_4:19709501-19709520 Msa0981020:five_prime_UTR 30.0%
ACCTAACACAATAGAATCAA+AGG + chr6_4:19709505-19709524 None:intergenic 30.0%
TCTTCTTGTACACATGTTAT+AGG - chr6_4:19709530-19709549 Msa0981020:five_prime_UTR 30.0%
TGAAAATGAGATCTTGTACT+TGG - chr6_4:19709581-19709600 Msa0981020:five_prime_UTR 30.0%
! AGGGAGGAATTTTCTATAAG+AGG + chr6_4:19705941-19705960 None:intergenic 35.0%
GTAAGAAGAAGCTAGGAAAA+TGG + chr6_4:19706216-19706235 None:intergenic 35.0%
TGAACAAGTAAGAAGAAGCT+AGG + chr6_4:19706223-19706242 None:intergenic 35.0%
! ACAACTGTATCTGGTTTCAA+TGG + chr6_4:19706611-19706630 None:intergenic 35.0%
TGCACTCATAGTTTCTTCAT+CGG + chr6_4:19706681-19706700 None:intergenic 35.0%
ATTATGATCGTGCTTTGCAA+TGG - chr6_4:19706765-19706784 Msa0981020:CDS 35.0%
TTATGATCGTGCTTTGCAAT+GGG - chr6_4:19706766-19706785 Msa0981020:CDS 35.0%
CGTTATAACTTCAAATCTGG+TGG - chr6_4:19706806-19706825 Msa0981020:CDS 35.0%
AATTGTTGTGGATGTGATCA+TGG - chr6_4:19706898-19706917 Msa0981020:CDS 35.0%
ACATATAAGTGGAAGGATCA+GGG + chr6_4:19707074-19707093 None:intergenic 35.0%
AGTTCACACATATAAGTGGA+AGG + chr6_4:19707081-19707100 None:intergenic 35.0%
ATTGAACTCAGAGAGTGATA+TGG - chr6_4:19707522-19707541 Msa0981020:intron 35.0%
! CAGAGAGTGATATGGAATTT+TGG - chr6_4:19707530-19707549 Msa0981020:intron 35.0%
!!! TTTTTTATATGGCTGGCCAA+TGG - chr6_4:19707604-19707623 Msa0981020:intron 35.0%
!! GAACAAAAAGCATTTGCCAT+TGG + chr6_4:19707623-19707642 None:intergenic 35.0%
! TTTACGACGTATTCCCATTT+TGG - chr6_4:19708106-19708125 Msa0981020:intron 35.0%
!!! AGCTCCTGCATATTTTTTGT+TGG - chr6_4:19708246-19708265 Msa0981020:intron 35.0%
TTTGAATGTTCATTAGGGCA+GGG + chr6_4:19708478-19708497 None:intergenic 35.0%
! TTTTGAATGTTCATTAGGGC+AGG + chr6_4:19708479-19708498 None:intergenic 35.0%
! GGGCATTTTGAATGTTCATT+AGG + chr6_4:19708484-19708503 None:intergenic 35.0%
CAAAATGCCCTAAATCAAGT+TGG - chr6_4:19708494-19708513 Msa0981020:intron 35.0%
CACCTAATCCAACTTGATTT+AGG + chr6_4:19708505-19708524 None:intergenic 35.0%
! TGGATTAGGTGACTCAATTA+AGG - chr6_4:19708514-19708533 Msa0981020:intron 35.0%
TTCCATCAGCTGGAATATTT+AGG - chr6_4:19708591-19708610 Msa0981020:intron 35.0%
! GTCTCAGCATTGAAATCAAT+TGG - chr6_4:19708821-19708840 Msa0981020:CDS 35.0%
CCTACTTCTCCAACAAAAAT+TGG + chr6_4:19708860-19708879 None:intergenic 35.0%
! CCAATTTTTGTTGGAGAAGT+AGG - chr6_4:19708857-19708876 Msa0981020:CDS 35.0%
! TTTTTGTTGGAGAAGTAGGT+TGG - chr6_4:19708861-19708880 Msa0981020:CDS 35.0%
TCAATGTAACCTTGTCTTCT+TGG + chr6_4:19708977-19708996 None:intergenic 35.0%
CAATGTCTCTCAAAATTTCC+TGG + chr6_4:19709046-19709065 None:intergenic 35.0%
!! CAGGAAATTTTGAGAGACAT+TGG - chr6_4:19709044-19709063 Msa0981020:five_prime_UTR 35.0%
!! GGAAATTTTGAGAGACATTG+GGG - chr6_4:19709046-19709065 Msa0981020:five_prime_UTR 35.0%
GAAACATGCATGGGAAATTT+AGG + chr6_4:19709094-19709113 None:intergenic 35.0%
! GGATTAAACATGCACCATTT+TGG + chr6_4:19709175-19709194 None:intergenic 35.0%
AAGATGATCCATATCCAAGT+GGG + chr6_4:19709264-19709283 None:intergenic 35.0%
TGGATCATCTTGCAACAATT+TGG - chr6_4:19709273-19709292 Msa0981020:five_prime_UTR 35.0%
TGCAACAATTTGGATGCTAA+TGG - chr6_4:19709283-19709302 Msa0981020:five_prime_UTR 35.0%
!!! ACTTGGCTTGCAATTTTGAA+GGG - chr6_4:19709356-19709375 Msa0981020:five_prime_UTR 35.0%
ACAAAGAAGAGGATGAAGAA+GGG + chr6_4:19709444-19709463 None:intergenic 35.0%
AACAAAGAAGAGGATGAAGA+AGG + chr6_4:19709445-19709464 None:intergenic 35.0%
AAATGAAGGGAACAAAGAAG+AGG + chr6_4:19709455-19709474 None:intergenic 35.0%
ATGTGGTGTGAAAATAAGGG+AGG + chr6_4:19705957-19705976 None:intergenic 40.0%
AGAAGCTAGGAAAATGGAGT+GGG + chr6_4:19706210-19706229 None:intergenic 40.0%
AAGAAGCTAGGAAAATGGAG+TGG + chr6_4:19706211-19706230 None:intergenic 40.0%
! TTTTGGTATGTGGAAGGACT+TGG - chr6_4:19706557-19706576 Msa0981020:CDS 40.0%
GAAGAAACTATGAGTGCACT+TGG - chr6_4:19706683-19706702 Msa0981020:CDS 40.0%
AAACTATGAGTGCACTTGGT+GGG - chr6_4:19706687-19706706 Msa0981020:CDS 40.0%
!! TGGGTCAGGTATTGAAGTAA+TGG - chr6_4:19706706-19706725 Msa0981020:CDS 40.0%
CACATATAAGTGGAAGGATC+AGG + chr6_4:19707075-19707094 None:intergenic 40.0%
GTTATTCGATCTTGATCGGA+CGG + chr6_4:19707385-19707404 None:intergenic 40.0%
! GCTTTTTGTTCTGACATGGA+CGG - chr6_4:19707632-19707651 Msa0981020:intron 40.0%
! TTCCCATTTTGGATTGTACC+TGG - chr6_4:19708117-19708136 Msa0981020:intron 40.0%
! TCCCATTTTGGATTGTACCT+GGG - chr6_4:19708118-19708137 Msa0981020:intron 40.0%
ACCCAGGTACAATCCAAAAT+GGG + chr6_4:19708122-19708141 None:intergenic 40.0%
! ATTTTGGATTGTACCTGGGT+CGG - chr6_4:19708122-19708141 Msa0981020:intron 40.0%
CAAAAAATATGCAGGAGCTG+AGG + chr6_4:19708245-19708264 None:intergenic 40.0%
TGATACAGGTATGTAGCAGT+TGG - chr6_4:19708401-19708420 Msa0981020:intron 40.0%
!! GCCCTAAATCAAGTTGGATT+AGG - chr6_4:19708500-19708519 Msa0981020:intron 40.0%
!! ATCAGCATTTAAGGGTACTG+TGG + chr6_4:19708540-19708559 None:intergenic 40.0%
GGAACAGCATTGTTTGAATC+TGG + chr6_4:19708575-19708594 None:intergenic 40.0%
! GCATTTTTGTCACCATCTGT+GGG + chr6_4:19708887-19708906 None:intergenic 40.0%
! TGCATTTTTGTCACCATCTG+TGG + chr6_4:19708888-19708907 None:intergenic 40.0%
! CCTTTATTTGATGCAAGCCT+AGG + chr6_4:19708950-19708969 None:intergenic 40.0%
CCTAGGCTTGCATCAAATAA+AGG - chr6_4:19708947-19708966 Msa0981020:CDS 40.0%
! AAAGGTACACCAAGAAGACA+AGG - chr6_4:19708965-19708984 Msa0981020:CDS 40.0%
CTCAAAATTTCCTGGCTCAA+TGG + chr6_4:19709038-19709057 None:intergenic 40.0%
AGATGATCCATATCCAAGTG+GGG + chr6_4:19709263-19709282 None:intergenic 40.0%
CAAGATGATCCATATCCAAG+TGG + chr6_4:19709265-19709284 None:intergenic 40.0%
AGTGCAAGATCAGAATGACT+TGG - chr6_4:19709339-19709358 Msa0981020:five_prime_UTR 40.0%
!!! GACTTGGCTTGCAATTTTGA+AGG - chr6_4:19709355-19709374 Msa0981020:five_prime_UTR 40.0%
TGAGATCTTGTACTTGGAGA+TGG - chr6_4:19709587-19709606 Msa0981020:five_prime_UTR 40.0%
!! TGGAAGGACTTGGTGTGAAT+TGG - chr6_4:19706567-19706586 Msa0981020:CDS 45.0%
!! GGAAGGACTTGGTGTGAATT+GGG - chr6_4:19706568-19706587 Msa0981020:CDS 45.0%
!! GAAGGACTTGGTGTGAATTG+GGG - chr6_4:19706569-19706588 Msa0981020:CDS 45.0%
GAAACTATGAGTGCACTTGG+TGG - chr6_4:19706686-19706705 Msa0981020:CDS 45.0%
! ATCGGACGGTTGATATCATG+TGG + chr6_4:19707371-19707390 None:intergenic 45.0%
! TAGTTTAGGGCTACACCGTT+TGG - chr6_4:19708075-19708094 Msa0981020:intron 45.0%
GACCCAGGTACAATCCAAAA+TGG + chr6_4:19708123-19708142 None:intergenic 45.0%
! TTTTGGATTGTACCTGGGTC+GGG - chr6_4:19708123-19708142 Msa0981020:intron 45.0%
AGAGTACAGGGAGAGAGATT+GGG + chr6_4:19708160-19708179 None:intergenic 45.0%
! AAGAGTACAGGGAGAGAGAT+TGG + chr6_4:19708161-19708180 None:intergenic 45.0%
AATGTTCATTAGGGCAGGGA+AGG + chr6_4:19708474-19708493 None:intergenic 45.0%
AACAATGCTGTTCCATCAGC+TGG - chr6_4:19708581-19708600 Msa0981020:intron 45.0%
GGCCTAAATATTCCAGCTGA+TGG + chr6_4:19708596-19708615 None:intergenic 45.0%
GGGTAAATGTTGACCGTGAA+TGG + chr6_4:19708674-19708693 None:intergenic 45.0%
GATGCAAAATCCATTGAGCC+AGG - chr6_4:19709025-19709044 Msa0981020:five_prime_UTR 45.0%
TCTTGTACTTGGAGATGGTG+TGG - chr6_4:19709592-19709611 Msa0981020:five_prime_UTR 45.0%
ACTTCCGACCTTCTCGATCA+CGG + chr6_4:19707034-19707053 None:intergenic 50.0%
AGAAGGTCGGAAGTCGTGAT+CGG - chr6_4:19707040-19707059 Msa0981020:CDS 50.0%
! TGCCTGTATGCAGGCTTGTT+GGG - chr6_4:19708023-19708042 Msa0981020:intron 50.0%
AACCCAACAAGCCTGCATAC+AGG + chr6_4:19708028-19708047 None:intergenic 50.0%
AGTACAGGGAGAGAGATTGG+GGG + chr6_4:19708158-19708177 None:intergenic 50.0%
GAGTACAGGGAGAGAGATTG+GGG + chr6_4:19708159-19708178 None:intergenic 50.0%
! TTTGCTGATTGCACCCCACT+TGG - chr6_4:19709247-19709266 Msa0981020:five_prime_UTR 50.0%
GATTGCACCCCACTTGGATA+TGG - chr6_4:19709253-19709272 Msa0981020:five_prime_UTR 50.0%
! ATGAGTGCACTTGGTGGGTC+AGG - chr6_4:19706692-19706711 Msa0981020:CDS 55.0%
GAAGCCGTGATCGAGAAGGT+CGG - chr6_4:19707027-19707046 Msa0981020:CDS 55.0%
GAAGGTCGGAAGTCGTGATC+GGG - chr6_4:19707041-19707060 Msa0981020:CDS 55.0%
CTGGTTTCGTGCCTGTATGC+AGG - chr6_4:19708014-19708033 Msa0981020:intron 55.0%
GTGCCTGTATGCAGGCTTGT+TGG - chr6_4:19708022-19708041 Msa0981020:intron 55.0%
GTACAGGGAGAGAGATTGGG+GGG + chr6_4:19708157-19708176 None:intergenic 55.0%
! GAAGTAGGTTGGCCCACAGA+TGG - chr6_4:19708872-19708891 Msa0981020:CDS 55.0%
GTCGGAAGCCGTGATCGAGA+AGG - chr6_4:19707023-19707042 Msa0981020:CDS 60.0%
GGGGACATTAGACCCGACCC+AGG + chr6_4:19708138-19708157 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 19705817 19709683 19705817 ID=Msa0981020;Name=Msa0981020
chr6_4 mRNA 19705817 19709683 19705817 ID=Msa0981020-mRNA-1;Parent=Msa0981020;Name=Msa0981020-mRNA-1;_AED=0.00;_eAED=0.00;_QI=683|1|1|1|1|1|2|161|484
chr6_4 exon 19705817 19707091 19705817 ID=Msa0981020-mRNA-1:exon:6152;Parent=Msa0981020-mRNA-1
chr6_4 exon 19708660 19709683 19708660 ID=Msa0981020-mRNA-1:exon:6151;Parent=Msa0981020-mRNA-1
chr6_4 five_prime_UTR 19709001 19709683 19709001 ID=Msa0981020-mRNA-1:five_prime_utr;Parent=Msa0981020-mRNA-1
chr6_4 CDS 19708660 19709000 19708660 ID=Msa0981020-mRNA-1:cds;Parent=Msa0981020-mRNA-1
chr6_4 CDS 19705978 19707091 19705978 ID=Msa0981020-mRNA-1:cds;Parent=Msa0981020-mRNA-1
chr6_4 three_prime_UTR 19705817 19705977 19705817 ID=Msa0981020-mRNA-1:three_prime_utr;Parent=Msa0981020-mRNA-1
Gene Sequence

>Msa0981020

ATGGGGAAAAATTGGTTTTCAAGATGGGTTTTTATGTTTATGATGATGAGTACAATTTTTTGGTATGTGGAAGGACTTGGTGTGAATTGGGGAACACAAGCAACACATCCATTGAAACCAGATACAGTTGTACAAATGTTGAAAGATAATGGAATTGAAAAAGTGAAGTTATTTGATGCCGATGAAGAAACTATGAGTGCACTTGGTGGGTCAGGTATTGAAGTAATGGTTGCTATTCCTAATAATCAATTAGCTGAAATGAGTTATTATGATCGTGCTTTGCAATGGGTTAGAAAGAATGTTACTCGTTATAACTTCAAATCTGGTGGAGTAAATATCAAGTATGTAGCAGTTGGAAATGAGCCATTTTTGAAATCATACAACAATTCATTCTTGAATGTAACCTTCCCTGCCCTAATGAACATTCAAAATGCCCTAAATCAAGTTGGATTAGGTGACTCAATTAAGGCCACAGTACCCTTAAATGCTGATGTTTATGAATCTCCAGATTCAAACAATGCTGTTCCATCAGCTGGAATATTTAGGCCTGATTTAAGTGAACTTATGACACAAATAGTCCAATTTTTGAGCAAAAATAATGCACCATTCACGGTCAACATTTACCCTTTTTTAAGTCTTTATGGAAATGACAATTTTCCATTTGACTTTGCATTTTTTGATGGAGTATCAAACCCTATAAATGACAATGGAATTTTGTACACAAATGTATTTGATGCAAATTTTGATACTTTAGTCTCAGCATTGAAATCAATTGGATTTGGTAACATTCCAATTTTTGTTGGAGAAGTAGGTTGGCCCACAGATGGTGACAAAAATGCAAATATTCAAAATGCATTAAGATTCTATAATGGTCTTTTACCTAGGCTTGCATCAAATAAAGGTACACCAAGAAGACAAGGTTACATTGAAGTTTATTTATTTGGATTAATTGATGAAGATGCAAAATCCATTGAGCCAGGAAATTTTGAGAGACATTGGGGAATTTTTAGTTTTGATGGTAAACCTAAATTTCCCATGCATGTTTCAAATCAACATGGTCAAAATAAATTATTGGTTGGTGCAAAAGATGTGCATTATCTTGATCCAAAATGGTGCATGTTTAATCCAAATGCCAATAATTTAACCAACCTTGCAGATAATATTAATTATGCTTGCAGTTTTGCTGATTGCACCCCACTTGGATATGGATCATCTTGCAACAATTTGGATGCTAATGGAAATGCTTCATATGCATTTAATATGTATTATCAAGTGCAAGATCAGAATGACTTGGCTTGCAATTTTGAAGGGTTAGCTATGATTACAACAAATAATATTTCAACTCCTACTTGTGATTTTATTATTCAAATTAACCCTTCTTCATCCTCTTCTTTGTTCCCTTCATTTGTTGCTTTCTTGTTTTTTAGTATACTACCTTTGATTCTATTGTGTTAG
Protein sequence

>Msa0981020

MGKNWFSRWVFMFMMMSTIFWYVEGLGVNWGTQATHPLKPDTVVQMLKDNGIEKVKLFDADEETMSALGGSGIEVMVAIPNNQLAEMSYYDRALQWVRKNVTRYNFKSGGVNIKYVAVGNEPFLKSYNNSFLNVTFPALMNIQNALNQVGLGDSIKATVPLNADVYESPDSNNAVPSAGIFRPDLSELMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDFAFFDGVSNPINDNGILYTNVFDANFDTLVSALKSIGFGNIPIFVGEVGWPTDGDKNANIQNALRFYNGLLPRLASNKGTPRRQGYIEVYLFGLIDEDAKSIEPGNFERHWGIFSFDGKPKFPMHVSNQHGQNKLLVGAKDVHYLDPKWCMFNPNANNLTNLADNINYACSFADCTPLGYGSSCNNLDANGNASYAFNMYYQVQDQNDLACNFEGLAMITTNNISTPTCDFIIQINPSSSSSLFPSFVAFLFFSILPLILLC*