AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1399420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1399420 MtrunA17_Chr6g0461601 97.521 484 12 0 1 484 1 484 0.0 974
Msa1399420 MtrunA17_Chr7g0253091 76.556 482 110 3 1 482 1 479 0.0 749
Msa1399420 MtrunA17_Chr4g0062771 58.405 464 186 5 10 472 18 475 0.0 542
Msa1399420 MtrunA17_Chr1g0148391 56.276 478 197 6 6 478 6 476 0.0 524
Msa1399420 MtrunA17_Chr2g0306311 51.697 501 208 7 10 483 18 511 1.06e-175 503
Msa1399420 MtrunA17_Chr3g0138741 56.044 455 192 5 10 462 15 463 2.83e-174 498
Msa1399420 MtrunA17_Chr8g0383311 55.275 436 189 4 23 457 40 470 1.36e-171 492
Msa1399420 MtrunA17_Chr4g0069801 52.318 453 200 7 25 471 27 469 2.43e-160 463
Msa1399420 MtrunA17_Chr4g0076581 49.687 479 227 6 10 483 17 486 1.69e-157 456
Msa1399420 MtrunA17_Chr3g0129341 52.477 444 203 6 23 465 31 467 5.68e-154 447
Msa1399420 MtrunA17_Chr2g0290821 50.575 435 204 6 26 457 28 454 5.79e-152 442
Msa1399420 MtrunA17_Chr3g0105981 46.154 416 213 8 24 438 25 430 9.46e-115 345
Msa1399420 MtrunA17_Chr3g0144531 33.787 441 265 16 26 453 28 454 5.62e-62 209
Msa1399420 MtrunA17_Chr4g0034901 33.262 466 275 18 9 453 9 459 8.48e-62 208
Msa1399420 MtrunA17_Chr1g0161011 32.609 460 272 17 10 453 8 445 7.55e-61 207
Msa1399420 MtrunA17_Chr8g0389741 30.455 440 281 14 26 454 28 453 5.48e-58 199
Msa1399420 MtrunA17_Chr7g0223961 33.259 448 259 18 23 451 20 446 7.16e-58 197
Msa1399420 MtrunA17_Chr6g0487261 29.701 468 290 17 9 454 5 455 2.13e-55 192
Msa1399420 MtrunA17_Chr5g0439251 31.957 460 251 17 26 447 25 460 4.24e-55 191
Msa1399420 MtrunA17_Chr1g0177171 30.426 470 268 18 26 453 38 490 3.30e-53 186
Msa1399420 MtrunA17_Chr4g0046311 31.057 454 276 19 26 457 23 461 7.59e-53 185
Msa1399420 MtrunA17_Chr3g0110371 29.694 458 288 18 10 453 8 445 6.74e-51 180
Msa1399420 MtrunA17_Chr4g0038981 32.153 339 208 9 10 344 4 324 7.38e-51 176
Msa1399420 MtrunA17_Chr4g0039011 33.742 326 194 10 26 349 42 347 1.13e-50 176
Msa1399420 MtrunA17_Chr1g0146831 34.615 364 206 15 11 357 16 364 1.57e-50 176
Msa1399420 MtrunA17_Chr4g0003281 34.203 345 197 13 13 340 20 351 4.27e-50 176
Msa1399420 MtrunA17_Chr4g0038941 33.636 330 196 9 20 346 46 355 4.75e-50 174
Msa1399420 MtrunA17_Chr4g0038961 32.153 339 208 9 10 344 4 324 7.92e-50 173
Msa1399420 MtrunA17_Chr8g0339191 29.478 441 285 15 27 453 30 458 1.26e-49 176
Msa1399420 MtrunA17_Chr7g0250301 33.538 325 196 11 26 346 8 316 3.28e-49 171
Msa1399420 MtrunA17_Chr4g0039071 32.722 327 202 9 24 346 23 335 5.64e-49 172
Msa1399420 MtrunA17_Chr5g0434591 29.464 448 270 16 26 453 23 444 9.54e-49 174
Msa1399420 MtrunA17_Chr1g0189111 33.133 332 197 12 23 344 28 344 1.91e-48 172
Msa1399420 MtrunA17_Chr4g0043851 32.558 344 207 11 8 339 11 341 2.84e-48 171
Msa1399420 MtrunA17_Chr4g0064021 33.025 324 194 11 27 340 27 337 2.95e-48 171
Msa1399420 MtrunA17_Chr4g0038971 31.692 325 203 8 23 346 20 326 3.15e-48 169
Msa1399420 MtrunA17_Chr4g0043511 29.540 457 278 15 26 453 62 503 3.22e-48 174
Msa1399420 MtrunA17_Chr4g0075031 30.474 443 272 17 26 453 45 466 5.20e-48 173
Msa1399420 MtrunA17_Chr3g0119541 36.061 330 180 13 26 341 26 338 6.81e-48 169
Msa1399420 MtrunA17_Chr8g0378621 28.846 468 293 19 3 453 4 448 8.22e-48 172
Msa1399420 MtrunA17_Chr2g0294591 32.609 322 203 6 26 345 30 339 1.70e-47 168
Msa1399420 MtrunA17_Chr2g0294601 31.988 322 205 6 26 345 59 368 4.18e-47 167
Msa1399420 MtrunA17_Chr3g0121271 29.067 461 292 17 26 472 26 465 3.78e-46 167
Msa1399420 MtrunA17_Chr4g0039021 33.882 304 180 10 46 344 1 288 1.37e-44 158
Msa1399420 MtrunA17_Chr2g0321511 29.805 359 224 12 8 354 9 351 1.75e-43 157
Msa1399420 MtrunA17_Chr4g0026321 31.965 341 206 13 16 344 17 343 2.88e-43 157
Msa1399420 MtrunA17_Chr7g0234821 27.416 445 292 17 27 453 27 458 1.15e-42 157
Msa1399420 MtrunA17_Chr2g0285901 33.333 324 189 12 27 339 25 332 4.50e-42 154
Msa1399420 MtrunA17_Chr1g0174261 32.653 294 180 9 55 344 1 280 2.41e-41 151
Msa1399420 MtrunA17_Chr1g0155001 31.157 337 210 11 26 352 24 348 3.57e-38 144
Msa1399420 MtrunA17_Chr7g0250321 32.852 277 170 9 24 297 26 289 2.49e-36 135
Msa1399420 MtrunA17_Chr5g0436361 29.073 313 192 14 42 344 56 348 3.85e-22 97.8
Msa1399420 MtrunA17_Chr2g0294581 34.074 135 85 2 26 159 76 207 8.02e-16 77.8
Msa1399420 MtrunA17_Chr2g0294581 38.298 94 52 3 251 343 190 278 4.94e-11 63.5
Msa1399420 MtrunA17_Chr4g0018771 33.333 93 59 2 252 344 84 173 5.39e-12 65.5
Msa1399420 MtrunA17_Chr4g0018861 28.571 133 72 3 234 346 44 173 1.73e-11 63.9
Msa1399420 MtrunA17_Chr8g0378401 45.000 80 41 3 371 448 32 110 7.24e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1399420 AT1G64760.1 73.563 435 111 4 23 457 22 452 0.0 647
Msa1399420 AT1G64760.2 73.563 435 111 4 23 457 22 452 0.0 647
Msa1399420 AT2G19440.2 71.269 449 123 5 9 457 6 448 0.0 644
Msa1399420 AT2G19440.1 71.269 449 123 5 9 457 6 448 0.0 644
Msa1399420 AT5G18220.1 66.094 466 145 7 23 483 27 484 0.0 613
Msa1399420 AT3G04010.1 66.813 455 143 6 12 462 16 466 0.0 613
Msa1399420 AT4G31140.1 53.374 489 212 7 3 483 4 484 0.0 516
Msa1399420 AT3G24330.1 54.077 466 200 7 26 481 39 500 4.80e-175 501
Msa1399420 AT5G58090.1 55.963 436 184 5 26 459 22 451 6.20e-174 498
Msa1399420 AT5G64790.1 54.545 451 199 4 12 461 14 459 5.90e-168 483
Msa1399420 AT5G20870.1 53.187 455 195 7 16 460 20 466 4.25e-157 456
Msa1399420 AT4G17180.1 48.980 441 209 7 26 464 25 451 1.56e-144 422
Msa1399420 AT5G58480.1 44.420 448 238 5 23 469 23 460 7.53e-123 367
Msa1399420 AT5G55180.1 32.743 452 258 16 26 453 27 456 2.56e-63 213
Msa1399420 AT3G55430.1 33.554 453 260 20 18 453 21 449 1.59e-62 210
Msa1399420 AT5G55180.2 32.644 435 261 15 26 443 27 446 1.99e-62 211
Msa1399420 AT2G05790.1 32.972 461 259 19 26 453 25 468 5.98e-62 209
Msa1399420 AT2G16230.1 34.004 447 250 18 26 451 25 447 2.57e-60 206
Msa1399420 AT4G26830.1 31.064 470 279 17 6 453 5 451 1.45e-58 200
Msa1399420 AT4G26830.2 31.778 450 262 17 26 453 29 455 1.08e-57 198
Msa1399420 AT2G39640.1 32.755 461 266 20 11 455 12 444 6.39e-57 198
Msa1399420 AT2G01630.1 31.448 442 269 18 26 453 23 444 7.06e-57 197
Msa1399420 AT1G66250.1 31.519 441 269 16 26 453 32 452 7.36e-56 194
Msa1399420 AT2G01630.3 31.193 436 266 18 26 447 23 438 1.17e-55 192
Msa1399420 AT5G24318.1 29.274 468 281 20 12 453 12 455 1.79e-55 192
Msa1399420 AT5G24318.2 29.476 458 273 20 12 443 12 445 9.60e-55 190
Msa1399420 AT4G16260.1 34.043 329 199 9 24 349 22 335 6.84e-53 182
Msa1399420 AT4G14080.1 30.349 458 289 17 13 453 29 473 1.29e-52 185
Msa1399420 AT4G34480.1 31.973 441 268 16 26 453 26 447 2.03e-52 185
Msa1399420 AT5G56590.1 28.412 447 292 13 22 454 22 454 5.33e-52 184
Msa1399420 AT3G23770.1 30.769 455 284 17 18 453 29 471 5.70e-52 183
Msa1399420 AT4G16260.2 34.286 315 190 8 37 349 7 306 6.52e-52 178
Msa1399420 AT3G07320.1 30.700 443 278 18 27 453 26 455 1.80e-51 181
Msa1399420 AT3G57270.1 35.093 322 193 9 26 344 27 335 2.24e-51 178
Msa1399420 AT3G57260.1 32.743 339 205 11 10 344 15 334 5.42e-51 177
Msa1399420 AT3G57260.2 32.743 339 205 11 10 344 31 350 7.40e-51 177
Msa1399420 AT1G11820.2 30.023 443 274 17 26 453 44 465 2.75e-50 179
Msa1399420 AT3G13560.4 30.110 455 285 14 26 467 26 460 5.59e-50 178
Msa1399420 AT3G13560.1 30.110 455 285 14 26 467 26 460 5.59e-50 178
Msa1399420 AT3G13560.2 30.110 455 285 14 26 467 26 460 5.59e-50 178
Msa1399420 AT3G13560.5 30.110 455 285 14 26 467 26 460 5.59e-50 178
Msa1399420 AT3G13560.3 30.110 455 285 14 26 467 26 460 5.59e-50 178
Msa1399420 AT1G11820.3 29.977 437 270 17 26 447 44 459 3.34e-49 176
Msa1399420 AT3G15800.1 33.234 334 199 12 18 340 35 355 1.13e-48 172
Msa1399420 AT4G18340.2 31.653 357 214 12 1 340 1 344 3.87e-48 170
Msa1399420 AT4G18340.1 31.897 348 210 11 7 340 10 344 1.12e-47 169
Msa1399420 AT1G30080.2 32.544 338 202 11 13 339 23 345 3.12e-47 168
Msa1399420 AT1G30080.1 32.544 338 202 11 13 339 23 345 5.04e-47 168
Msa1399420 AT1G32860.1 32.822 326 195 10 26 340 29 341 4.69e-46 166
Msa1399420 AT4G29360.1 25.988 481 309 15 9 455 10 477 4.25e-45 166
Msa1399420 AT2G01630.2 31.940 335 205 12 26 349 23 345 4.41e-45 162
Msa1399420 AT2G26600.1 29.972 357 225 12 22 366 30 373 6.04e-45 162
Msa1399420 AT3G57240.1 31.692 325 199 9 23 344 32 336 9.09e-45 160
Msa1399420 AT4G29360.2 25.866 491 311 16 5 455 10 487 1.78e-43 161
Msa1399420 AT5G42720.1 34.462 325 189 13 26 339 26 337 3.11e-43 159
Msa1399420 AT4G34480.2 36.111 324 184 13 26 339 26 336 5.02e-43 156
Msa1399420 AT5G24318.4 30.636 346 212 14 12 344 12 342 6.28e-43 156
Msa1399420 AT5G42100.2 33.923 339 197 13 23 348 24 348 9.76e-42 154
Msa1399420 AT5G42100.1 33.923 339 197 13 23 348 24 348 1.40e-41 154
Msa1399420 AT3G61810.1 28.994 338 206 12 26 349 55 372 8.49e-41 150
Msa1399420 AT2G27500.2 30.211 331 208 11 24 344 27 344 3.30e-40 149
Msa1399420 AT2G27500.1 30.211 331 208 11 24 344 27 344 6.72e-40 148
Msa1399420 AT4G34480.3 32.571 350 208 14 116 453 3 336 2.43e-38 144
Msa1399420 AT1G77780.1 28.923 325 209 9 27 346 25 332 3.46e-38 143
Msa1399420 AT1G11820.1 29.532 342 220 10 26 357 44 374 6.93e-38 143
Msa1399420 AT5G20390.1 30.488 328 204 11 26 346 33 343 4.52e-36 137
Msa1399420 AT1G77790.1 29.143 350 218 12 8 344 7 339 8.06e-36 136
Msa1399420 AT5G24318.3 29.394 330 204 14 139 453 1 316 3.76e-35 134
Msa1399420 AT5G20330.1 30.183 328 205 11 26 346 34 344 1.21e-33 130
Msa1399420 AT5G20340.1 29.653 317 200 10 26 336 43 342 1.33e-33 130
Msa1399420 AT1G33220.1 30.033 303 192 9 26 322 34 322 5.04e-33 128
Msa1399420 AT2G26600.2 30.502 259 158 10 91 340 6 251 1.24e-30 120
Msa1399420 AT3G46570.1 26.611 357 231 13 1 344 2 340 2.05e-30 121
Msa1399420 AT2G27500.3 30.108 279 172 11 76 344 1 266 3.78e-30 120
Msa1399420 AT3G55780.1 26.205 332 219 9 26 344 31 349 1.98e-29 119
Msa1399420 AT5G20560.1 27.129 317 209 10 26 336 34 334 3.88e-28 114
Msa1399420 AT2G43670.1 39.024 82 48 2 371 451 35 115 1.57e-11 62.0

Find 103 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACATTGAAGTTTATTTATT+TGG 0.091611 tig0022357:+63039 Msa1399420:CDS
TTCATAAACATCAGCATTTA+AGG 0.121944 tig0022357:-62596 None:intergenic
TGCAAGGTTGCTTAAATTAT+TGG 0.148615 tig0022357:-63250 None:intergenic
GAAACATGCATGGGAAATTT+AGG 0.155283 tig0022357:-63141 None:intergenic
CACCTAATCCAACTTGATTT+AGG 0.179677 tig0022357:-62552 None:intergenic
TCTTCTTGTACACATGTTAT+AGG 0.180643 tig0022357:+63580 Msa1399420:three_prime_UTR
GGATTAAACATGCACCATTT+TGG 0.186507 tig0022357:-63222 None:intergenic
TATCTTTGAGTGAAAATTAA+TGG 0.197627 tig0022357:+60547 Msa1399420:exon
ATTTCAGCTAATTGATTATT+AGG 0.201538 tig0022357:-60803 None:intergenic
TGGGAATATATATGATAAAA+AGG 0.212478 tig0022357:-60253 None:intergenic
ATGGATCATTAGATGGAATT+TGG 0.214314 tig0022357:-59960 None:intergenic
TCAATGTAACCTTGTCTTCT+TGG 0.216980 tig0022357:-63024 None:intergenic
TGGATCATCTTGCAACAATT+TGG 0.228531 tig0022357:+63323 Msa1399420:CDS
ACATGGTCAAAATAAATTAT+TGG 0.229515 tig0022357:+63170 Msa1399420:CDS
AACATTTGTACAACTGTATC+TGG 0.259099 tig0022357:-60683 None:intergenic
AAATTCCATTGTCATTTATA+GGG 0.260086 tig0022357:-62810 None:intergenic
AAAATTCCATTGTCATTTAT+AGG 0.268255 tig0022357:-62811 None:intergenic
ATGTATGTGGTGTGAAAATA+AGG 0.275216 tig0022357:-60025 None:intergenic
GAAGTATGATAATCTAATTA+AGG 0.279119 tig0022357:-60072 None:intergenic
ATCTTTGAGTGAAAATTAAT+GGG 0.281401 tig0022357:+60548 Msa1399420:exon
AGCACTTCTATATTTGTATA+TGG 0.290955 tig0022357:-60469 None:intergenic
ATATTCTATGTTTGATTGAT+TGG 0.294741 tig0022357:-60151 None:intergenic
GTAAGAAGAAGCTAGGAAAA+TGG 0.309827 tig0022357:-60278 None:intergenic
TTCCATCAGCTGGAATATTT+AGG 0.313567 tig0022357:+62641 Msa1399420:CDS
ACCTAATCCAACTTGATTTA+GGG 0.313740 tig0022357:-62551 None:intergenic
CCTAGGCTTGCATCAAATAA+AGG 0.313854 tig0022357:+62997 Msa1399420:CDS
GGAAGGACTTGGTGTGAATT+GGG 0.317702 tig0022357:+60634 Msa1399420:CDS
TGCAACAATTTGGATGCTAA+TGG 0.318329 tig0022357:+63333 Msa1399420:CDS
TGGAAGGACTTGGTGTGAAT+TGG 0.327363 tig0022357:+60633 Msa1399420:CDS
TTGCTTAAATTATTGGCATT+TGG 0.329109 tig0022357:-63243 None:intergenic
GATTGCACCCCACTTGGATA+TGG 0.332800 tig0022357:+63303 Msa1399420:CDS
GCATTGAAATCAATTGGATT+TGG 0.334676 tig0022357:+62877 Msa1399420:CDS
TGGATTAGGTGACTCAATTA+AGG 0.336092 tig0022357:+62564 Msa1399420:CDS
ACCTTTGATTCTATTGTGTT+AGG 0.337915 tig0022357:+63551 Msa1399420:CDS
CTCAAAATTTCCTGGCTCAA+TGG 0.343066 tig0022357:-63085 None:intergenic
GTCTCAGCATTGAAATCAAT+TGG 0.343874 tig0022357:+62871 Msa1399420:CDS
GTACAAATGTTGAAAGATAA+TGG 0.351290 tig0022357:+60695 Msa1399420:CDS
TGCATTATCTTGATCCAAAA+TGG 0.352525 tig0022357:+63208 Msa1399420:CDS
CAATGTCTCTCAAAATTTCC+TGG 0.367879 tig0022357:-63093 None:intergenic
GGAACAGCATTGTTTGAATC+TGG 0.376124 tig0022357:-62622 None:intergenic
ACTCGTTATAACTTCAAATC+TGG 0.376451 tig0022357:+60869 Msa1399420:CDS
AAGTATGATAATCTAATTAA+GGG 0.381909 tig0022357:-60071 None:intergenic
GTCATAAGTTCACTTAAATC+AGG 0.389740 tig0022357:-62664 None:intergenic
TATTCTATGTTTGATTGATT+GGG 0.392169 tig0022357:-60150 None:intergenic
ACTAGAAATGGATCATTAGA+TGG 0.397875 tig0022357:-59967 None:intergenic
AAGAAGAAGAAAACTAGAAA+TGG 0.410233 tig0022357:-59979 None:intergenic
AACAATGCTGTTCCATCAGC+TGG 0.414573 tig0022357:+62631 Msa1399420:CDS
AACAAGAAAGCAACAAATGA+AGG 0.414915 tig0022357:-63516 None:intergenic
ATGAGTGCACTTGGTGGGTC+AGG 0.429886 tig0022357:+60758 Msa1399420:CDS
GCCCTAAATCAAGTTGGATT+AGG 0.441972 tig0022357:+62550 Msa1399420:CDS
ATAGGAGTGAAATTTATTAG+TGG 0.469236 tig0022357:-60346 None:intergenic
TTATGATCGTGCTTTGCAAT+GGG 0.469916 tig0022357:+60832 Msa1399420:CDS
AATGCATTAAGATTCTATAA+TGG 0.474784 tig0022357:+62967 Msa1399420:CDS
TGTTACAGGTATGTAGCAGT+TGG 0.485450 tig0022357:+62451 Msa1399420:intron
AACAAAGAAGAGGATGAAGA+AGG 0.489426 tig0022357:-63492 None:intergenic
TGAAAATGAGATCTTGTACT+TGG 0.497057 tig0022357:+63631 Msa1399420:three_prime_UTR
GGCCTAAATATTCCAGCTGA+TGG 0.497077 tig0022357:-62643 None:intergenic
CCTTTATTTGATGCAAGCCT+AGG 0.497501 tig0022357:-62997 None:intergenic
TCATAAACATCAGCATTTAA+GGG 0.503342 tig0022357:-62595 None:intergenic
TATTTAGAGATGATTAAGAA+AGG 0.506239 tig0022357:-59936 None:intergenic
ACAACTGTATCTGGTTTCAA+TGG 0.506632 tig0022357:-60674 None:intergenic
CAAAATGCCCTAAATCAAGT+TGG 0.507329 tig0022357:+62544 Msa1399420:CDS
TTTGCTGATTGCACCCCACT+TGG 0.508951 tig0022357:+63297 Msa1399420:CDS
ACAAAGAAGAGGATGAAGAA+GGG 0.509172 tig0022357:-63491 None:intergenic
AAGAAGCTAGGAAAATGGAG+TGG 0.511243 tig0022357:-60273 None:intergenic
GGGTAAATGTTGACCGTGAA+TGG 0.513460 tig0022357:-62721 None:intergenic
ATTATGATCGTGCTTTGCAA+TGG 0.517702 tig0022357:+60831 Msa1399420:CDS
GGTCAAAATAAATTATTGGT+TGG 0.518395 tig0022357:+63174 Msa1399420:CDS
TGAACAAGTAAGAAGAAGCT+AGG 0.523091 tig0022357:-60285 None:intergenic
CATAATTAATATTATCTGCA+AGG 0.534309 tig0022357:-63266 None:intergenic
TGAATAATAAAATCACAAGT+AGG 0.543706 tig0022357:-63462 None:intergenic
AATGTTCATTAGGGCAGGGA+AGG 0.547103 tig0022357:-62521 None:intergenic
GATGCAAAATCCATTGAGCC+AGG 0.551835 tig0022357:+63075 Msa1399420:CDS
AGAAGCTAGGAAAATGGAGT+GGG 0.553250 tig0022357:-60272 None:intergenic
ATTCTATGTTTGATTGATTG+GGG 0.553940 tig0022357:-60149 None:intergenic
TGGGTCAGGTATTGAAGTAA+TGG 0.555721 tig0022357:+60772 Msa1399420:CDS
ACCTAACACAATAGAATCAA+AGG 0.557731 tig0022357:-63552 None:intergenic
CGTTATAACTTCAAATCTGG+TGG 0.565387 tig0022357:+60872 Msa1399420:CDS
GAAGTAGGTTGGCCCACAGA+TGG 0.567464 tig0022357:+62922 Msa1399420:CDS
ACTTCTATATTTGTATATGG+TGG 0.568585 tig0022357:-60466 None:intergenic
TCTTTGAGTGAAAATTAATG+GGG 0.578888 tig0022357:+60549 Msa1399420:exon
AAACTATGAGTGCACTTGGT+GGG 0.583255 tig0022357:+60753 Msa1399420:CDS
ACAAGAAAGCAACAAATGAA+GGG 0.589905 tig0022357:-63515 None:intergenic
TCAAACCCTATAAATGACAA+TGG 0.600273 tig0022357:+62805 Msa1399420:CDS
TGTATGTGGTGTGAAAATAA+GGG 0.608924 tig0022357:-60024 None:intergenic
TGAGATCTTGTACTTGGAGA+TGG 0.616005 tig0022357:+63637 Msa1399420:three_prime_UTR
GAAGGACTTGGTGTGAATTG+GGG 0.620435 tig0022357:+60635 Msa1399420:CDS
AAGATGATCCATATCCAAGT+GGG 0.621191 tig0022357:-63311 None:intergenic
AAGTATATGAATTATGTATG+TGG 0.630200 tig0022357:-60038 None:intergenic
TCTTGTACTTGGAGATGGTG+TGG 0.634220 tig0022357:+63642 Msa1399420:three_prime_UTR
ATGTTGATTTGAAACATGCA+TGG 0.636846 tig0022357:-63151 None:intergenic
AAATGAAGGGAACAAAGAAG+AGG 0.637162 tig0022357:-63502 None:intergenic
AGTGCAAGATCAGAATGACT+TGG 0.640166 tig0022357:+63389 Msa1399420:CDS
GAAGAAACTATGAGTGCACT+TGG 0.642757 tig0022357:+60749 Msa1399420:CDS
TTTGAATGTTCATTAGGGCA+GGG 0.642889 tig0022357:-62525 None:intergenic
TGTTGATTTGAAACATGCAT+GGG 0.655324 tig0022357:-63150 None:intergenic
ATCAGCATTTAAGGGTACTG+TGG 0.661565 tig0022357:-62587 None:intergenic
GAAACTATGAGTGCACTTGG+TGG 0.667838 tig0022357:+60752 Msa1399420:CDS
AAAGGTACACCAAGAAGACA+AGG 0.670968 tig0022357:+63015 Msa1399420:CDS
CAAGATGATCCATATCCAAG+TGG 0.675663 tig0022357:-63312 None:intergenic
AGATGATCCATATCCAAGTG+GGG 0.711276 tig0022357:-63310 None:intergenic
ATGTGGTGTGAAAATAAGGG+AGG 0.718069 tig0022357:-60021 None:intergenic
ATGCATGTTTCAAATCAACA+TGG 0.723172 tig0022357:+63153 Msa1399420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATATACTAGTTAATA+TGG + tig0022357:61185-61204 Msa1399420:intron 10.0%
!! AAGTATGATAATCTAATTAA+GGG - tig0022357:60074-60093 None:intergenic 15.0%
!!! CTTTTTTTAATGTCAATTAT+TGG + tig0022357:61262-61281 Msa1399420:intron 15.0%
!! TTTGAACAATAAACTAAAAT+TGG - tig0022357:61470-61489 None:intergenic 15.0%
!!! CTGCATTTTTTTTTTTTATA+TGG + tig0022357:61645-61664 Msa1399420:intron 15.0%
!!! ATTTTTTTTTTTTATATGGC+TGG + tig0022357:61649-61668 Msa1399420:intron 15.0%
!!! GACTAAAATTCATATTTTTA+AGG + tig0022357:61994-62013 Msa1399420:intron 15.0%
!!! ATATTTTTAAGGTGAATAAA+CGG + tig0022357:62005-62024 Msa1399420:intron 15.0%
!!! AATTTTTGTTAGAATAGTTT+AGG + tig0022357:62112-62131 Msa1399420:intron 15.0%
!!! ATTTTTGTTAGAATAGTTTA+GGG + tig0022357:62113-62132 Msa1399420:intron 15.0%
!! TACATTGAAGTTTATTTATT+TGG + tig0022357:63039-63058 Msa1399420:CDS 15.0%
!!! ACTTGAATATATTATTTTGA+AGG + tig0022357:63707-63726 Msa1399420:three_prime_UTR 15.0%
!!! CTTGAATATATTATTTTGAA+GGG + tig0022357:63708-63727 Msa1399420:three_prime_UTR 15.0%
!! TATTTAGAGATGATTAAGAA+AGG - tig0022357:59939-59958 None:intergenic 20.0%
!! AAGTATATGAATTATGTATG+TGG - tig0022357:60041-60060 None:intergenic 20.0%
!! GAAGTATGATAATCTAATTA+AGG - tig0022357:60075-60094 None:intergenic 20.0%
!! TATTCTATGTTTGATTGATT+GGG - tig0022357:60153-60172 None:intergenic 20.0%
!! ATATTCTATGTTTGATTGAT+TGG - tig0022357:60154-60173 None:intergenic 20.0%
!! GGGAATATATATGATAAAAA+GGG - tig0022357:60255-60274 None:intergenic 20.0%
!! TGGGAATATATATGATAAAA+AGG - tig0022357:60256-60275 None:intergenic 20.0%
!! TATCTTTGAGTGAAAATTAA+TGG + tig0022357:60547-60566 Msa1399420:exon 20.0%
!! ATCTTTGAGTGAAAATTAAT+GGG + tig0022357:60548-60567 Msa1399420:exon 20.0%
!! ATTTCAGCTAATTGATTATT+AGG - tig0022357:60806-60825 None:intergenic 20.0%
!!! GTTTTTATTGTTAATTGTTG+TGG + tig0022357:60950-60969 Msa1399420:intron 20.0%
!! CAAAAAATGTCAAAACTATT+AGG + tig0022357:61065-61084 Msa1399420:intron 20.0%
!! TAAAACTGATAAGAAATGAT+CGG + tig0022357:61333-61352 Msa1399420:intron 20.0%
!! GAATATGATTCTATAAACTA+AGG + tig0022357:61378-61397 Msa1399420:intron 20.0%
!!! GATTCTTTTTCTTTTTTTCT+TGG + tig0022357:61775-61794 Msa1399420:intron 20.0%
!! AAACAAAACTAGATCTTAAT+TGG + tig0022357:61849-61868 Msa1399420:intron 20.0%
!!! TCATCATATTTTTTTGTAAC+TGG + tig0022357:62046-62065 Msa1399420:intron 20.0%
!!! TTCTTTGGCTTAAAAAAAAA+CGG + tig0022357:62253-62272 Msa1399420:intron 20.0%
!! GTGTTTAGGAAATAAATAAT+TGG - tig0022357:62400-62419 None:intergenic 20.0%
!! TAACAATAATAGTGTAAAGA+TGG - tig0022357:62436-62455 None:intergenic 20.0%
!! TTGTTGTATGATTTCAAAAA+TGG - tig0022357:62484-62503 None:intergenic 20.0%
!!! CATTATTTTTGCTCAAAAAT+TGG - tig0022357:62699-62718 None:intergenic 20.0%
!! TTCCATAAAGACTTAAAAAA+GGG - tig0022357:62744-62763 None:intergenic 20.0%
!! TTTCCATAAAGACTTAAAAA+AGG - tig0022357:62745-62764 None:intergenic 20.0%
!!! AAATTCCATTGTCATTTATA+GGG - tig0022357:62813-62832 None:intergenic 20.0%
!!! AAAATTCCATTGTCATTTAT+AGG - tig0022357:62814-62833 None:intergenic 20.0%
!! AATGCATTAAGATTCTATAA+TGG + tig0022357:62967-62986 Msa1399420:CDS 20.0%
!! ACATGGTCAAAATAAATTAT+TGG + tig0022357:63170-63189 Msa1399420:CDS 20.0%
!! CATAATTAATATTATCTGCA+AGG - tig0022357:63269-63288 None:intergenic 20.0%
!! TGAATAATAAAATCACAAGT+AGG - tig0022357:63465-63484 None:intergenic 20.0%
! AAGAAGAAGAAAACTAGAAA+TGG - tig0022357:59982-60001 None:intergenic 25.0%
! TATAAAAAGCTAGAACAAGT+TGG - tig0022357:60108-60127 None:intergenic 25.0%
! ATTCTATGTTTGATTGATTG+GGG - tig0022357:60152-60171 None:intergenic 25.0%
! ATAGGAGTGAAATTTATTAG+TGG - tig0022357:60349-60368 None:intergenic 25.0%
! TTTCCTTACACACTAAAAAT+AGG - tig0022357:60367-60386 None:intergenic 25.0%
! ACTTCTATATTTGTATATGG+TGG - tig0022357:60469-60488 None:intergenic 25.0%
! AGCACTTCTATATTTGTATA+TGG - tig0022357:60472-60491 None:intergenic 25.0%
! TCTTTGAGTGAAAATTAATG+GGG + tig0022357:60549-60568 Msa1399420:exon 25.0%
! GAAAATTAATGGGGAAAAAT+TGG + tig0022357:60558-60577 Msa1399420:exon 25.0%
!!! GAAAAATTGGTTTTCAAGAT+GGG + tig0022357:60571-60590 Msa1399420:CDS 25.0%
!!! TGATGATGAGTACAATTTTT+TGG + tig0022357:60606-60625 Msa1399420:CDS 25.0%
! GTACAAATGTTGAAAGATAA+TGG + tig0022357:60695-60714 Msa1399420:CDS 25.0%
! AAATGTCAAAACTATTAGGT+CGG + tig0022357:61069-61088 Msa1399420:intron 25.0%
!! TTTGATGTTATTTGAAGTTC+TGG - tig0022357:61149-61168 None:intergenic 25.0%
! TTAATGTCAATTATTGGACT+AGG + tig0022357:61268-61287 Msa1399420:intron 25.0%
!!! AATGATCGGTTGATAATTAT+TGG + tig0022357:61347-61366 Msa1399420:intron 25.0%
! AGGAAATCATACATTTCATA+TGG + tig0022357:61398-61417 Msa1399420:intron 25.0%
! TTATGTTTGAGTAGTTACTT+TGG + tig0022357:61618-61637 Msa1399420:intron 25.0%
! AACAAAAATTCACACATCAT+GGG - tig0022357:62102-62121 None:intergenic 25.0%
! TAACAAAAATTCACACATCA+TGG - tig0022357:62103-62122 None:intergenic 25.0%
! TCGTAAAAAATCAAACCAAA+CGG - tig0022357:62144-62163 None:intergenic 25.0%
!! AATAACAAATGAAGAGTACA+GGG - tig0022357:62223-62242 None:intergenic 25.0%
!! AAATAACAAATGAAGAGTAC+AGG - tig0022357:62224-62243 None:intergenic 25.0%
! ATTACCAACAAAAAATATGC+AGG - tig0022357:62303-62322 None:intergenic 25.0%
! TATTTATTTGTGTGTGAATC+AGG + tig0022357:62324-62343 Msa1399420:intron 25.0%
! ATTTATTTGTGTGTGAATCA+GGG + tig0022357:62325-62344 Msa1399420:intron 25.0%
! GAATGCATAAATATGTGTTT+AGG - tig0022357:62414-62433 None:intergenic 25.0%
! CTTTACACTATTATTGTTAC+AGG + tig0022357:62437-62456 Msa1399420:intron 25.0%
! TCATAAACATCAGCATTTAA+GGG - tig0022357:62598-62617 None:intergenic 25.0%
! TTCATAAACATCAGCATTTA+AGG - tig0022357:62599-62618 None:intergenic 25.0%
!!! TACCCTTTTTTAAGTCTTTA+TGG + tig0022357:62739-62758 Msa1399420:CDS 25.0%
! TCAAAAAATGCAAAGTCAAA+TGG - tig0022357:62778-62797 None:intergenic 25.0%
!!! TTTGACTTTGCATTTTTTGA+TGG + tig0022357:62778-62797 Msa1399420:CDS 25.0%
! GGTCAAAATAAATTATTGGT+TGG + tig0022357:63174-63193 Msa1399420:CDS 25.0%
! TTGCTTAAATTATTGGCATT+TGG - tig0022357:63246-63265 None:intergenic 25.0%
ATGGATCATTAGATGGAATT+TGG - tig0022357:59963-59982 None:intergenic 30.0%
ACTAGAAATGGATCATTAGA+TGG - tig0022357:59970-59989 None:intergenic 30.0%
TGTATGTGGTGTGAAAATAA+GGG - tig0022357:60027-60046 None:intergenic 30.0%
ATGTATGTGGTGTGAAAATA+AGG - tig0022357:60028-60047 None:intergenic 30.0%
!! AGTCATTTTCTTTCTTCACT+TGG + tig0022357:60188-60207 Msa1399420:five_prime_UTR 30.0%
!! GTCATTTTCTTTCTTCACTT+GGG + tig0022357:60189-60208 Msa1399420:five_prime_UTR 30.0%
! TCATTTTCTTTCTTCACTTG+GGG + tig0022357:60190-60209 Msa1399420:five_prime_UTR 30.0%
!!! ACTCCTATTTTTAGTGTGTA+AGG + tig0022357:60361-60380 Msa1399420:five_prime_UTR 30.0%
!!! GGAAAAATTGGTTTTCAAGA+TGG + tig0022357:60570-60589 Msa1399420:CDS 30.0%
!!! GAGTACAATTTTTTGGTATG+TGG + tig0022357:60613-60632 Msa1399420:CDS 30.0%
!!! ACAATTTTTTGGTATGTGGA+AGG + tig0022357:60617-60636 Msa1399420:CDS 30.0%
AACATTTGTACAACTGTATC+TGG - tig0022357:60686-60705 None:intergenic 30.0%
ACTCGTTATAACTTCAAATC+TGG + tig0022357:60869-60888 Msa1399420:CDS 30.0%
!!! TGGTTTTATATTTCACTTGC+TGG + tig0022357:60982-61001 Msa1399420:intron 30.0%
! TTTATATTTCACTTGCTGGT+TGG + tig0022357:60986-61005 Msa1399420:intron 30.0%
ATGAGTTATTCGATCTTGAT+CGG - tig0022357:61442-61461 None:intergenic 30.0%
!! AAATGCTTTTTGTTCTGACA+TGG + tig0022357:61680-61699 Msa1399420:intron 30.0%
CAAAACTAGATCTTAATTGG+TGG + tig0022357:61852-61871 Msa1399420:intron 30.0%
TTAAAAAAAAACGGTGTGTC+AGG + tig0022357:62262-62281 Msa1399420:intron 30.0%
! GGCATTTTGAATGTTCATTA+GGG - tig0022357:62533-62552 None:intergenic 30.0%
ACCTAATCCAACTTGATTTA+GGG - tig0022357:62554-62573 None:intergenic 30.0%
GTCATAAGTTCACTTAAATC+AGG - tig0022357:62667-62686 None:intergenic 30.0%
CAAAAATAATGCACCATTCA+CGG + tig0022357:62708-62727 Msa1399420:CDS 30.0%
TCAAACCCTATAAATGACAA+TGG + tig0022357:62805-62824 Msa1399420:CDS 30.0%
GCATTGAAATCAATTGGATT+TGG + tig0022357:62877-62896 Msa1399420:CDS 30.0%
! GGTAACATTCCAATTTTTGT+TGG + tig0022357:62898-62917 Msa1399420:CDS 30.0%
! TCTATAATGGTCTTTTACCT+AGG + tig0022357:62980-62999 Msa1399420:CDS 30.0%
!! AGGAAATTTTGAGAGACATT+GGG + tig0022357:63095-63114 Msa1399420:CDS 30.0%
!!! TGGGGAATTTTTAGTTTTGA+TGG + tig0022357:63114-63133 Msa1399420:CDS 30.0%
!! TGTTGATTTGAAACATGCAT+GGG - tig0022357:63153-63172 None:intergenic 30.0%
!! ATGTTGATTTGAAACATGCA+TGG - tig0022357:63154-63173 None:intergenic 30.0%
ATGCATGTTTCAAATCAACA+TGG + tig0022357:63153-63172 Msa1399420:CDS 30.0%
TGCATTATCTTGATCCAAAA+TGG + tig0022357:63208-63227 Msa1399420:CDS 30.0%
TGCAAGGTTGCTTAAATTAT+TGG - tig0022357:63253-63272 None:intergenic 30.0%
ACAAGAAAGCAACAAATGAA+GGG - tig0022357:63518-63537 None:intergenic 30.0%
AACAAGAAAGCAACAAATGA+AGG - tig0022357:63519-63538 None:intergenic 30.0%
ACCTTTGATTCTATTGTGTT+AGG + tig0022357:63551-63570 Msa1399420:CDS 30.0%
ACCTAACACAATAGAATCAA+AGG - tig0022357:63555-63574 None:intergenic 30.0%
TCTTCTTGTACACATGTTAT+AGG + tig0022357:63580-63599 Msa1399420:three_prime_UTR 30.0%
TGAAAATGAGATCTTGTACT+TGG + tig0022357:63631-63650 Msa1399420:three_prime_UTR 30.0%
! AGGGAGGAATTTTCTATAAG+AGG - tig0022357:60008-60027 None:intergenic 35.0%
GTAAGAAGAAGCTAGGAAAA+TGG - tig0022357:60281-60300 None:intergenic 35.0%
TGAACAAGTAAGAAGAAGCT+AGG - tig0022357:60288-60307 None:intergenic 35.0%
! ACAACTGTATCTGGTTTCAA+TGG - tig0022357:60677-60696 None:intergenic 35.0%
ATTATGATCGTGCTTTGCAA+TGG + tig0022357:60831-60850 Msa1399420:CDS 35.0%
TTATGATCGTGCTTTGCAAT+GGG + tig0022357:60832-60851 Msa1399420:CDS 35.0%
CGTTATAACTTCAAATCTGG+TGG + tig0022357:60872-60891 Msa1399420:CDS 35.0%
AATTGTTGTGGATGTGATCA+TGG + tig0022357:60962-60981 Msa1399420:intron 35.0%
ATTGAACTCAGAGAGTGATA+TGG + tig0022357:61575-61594 Msa1399420:intron 35.0%
! CAGAGAGTGATATGGAATTT+TGG + tig0022357:61583-61602 Msa1399420:intron 35.0%
!!! TTTTTTATATGGCTGGCCAA+TGG + tig0022357:61656-61675 Msa1399420:intron 35.0%
!! GAACAAAAAGCATTTGCCAT+TGG - tig0022357:61675-61694 None:intergenic 35.0%
! TTTACGACGTATTCCCATTT+TGG + tig0022357:62157-62176 Msa1399420:intron 35.0%
!!! AGCTCCTGCATATTTTTTGT+TGG + tig0022357:62296-62315 Msa1399420:intron 35.0%
TTTGAATGTTCATTAGGGCA+GGG - tig0022357:62528-62547 None:intergenic 35.0%
! TTTTGAATGTTCATTAGGGC+AGG - tig0022357:62529-62548 None:intergenic 35.0%
! GGGCATTTTGAATGTTCATT+AGG - tig0022357:62534-62553 None:intergenic 35.0%
CAAAATGCCCTAAATCAAGT+TGG + tig0022357:62544-62563 Msa1399420:CDS 35.0%
CACCTAATCCAACTTGATTT+AGG - tig0022357:62555-62574 None:intergenic 35.0%
! TGGATTAGGTGACTCAATTA+AGG + tig0022357:62564-62583 Msa1399420:CDS 35.0%
TTCCATCAGCTGGAATATTT+AGG + tig0022357:62641-62660 Msa1399420:CDS 35.0%
! GTCTCAGCATTGAAATCAAT+TGG + tig0022357:62871-62890 Msa1399420:CDS 35.0%
CCTACTTCTCCAACAAAAAT+TGG - tig0022357:62910-62929 None:intergenic 35.0%
! CCAATTTTTGTTGGAGAAGT+AGG + tig0022357:62907-62926 Msa1399420:CDS 35.0%
! TTTTTGTTGGAGAAGTAGGT+TGG + tig0022357:62911-62930 Msa1399420:CDS 35.0%
TCAATGTAACCTTGTCTTCT+TGG - tig0022357:63027-63046 None:intergenic 35.0%
CAATGTCTCTCAAAATTTCC+TGG - tig0022357:63096-63115 None:intergenic 35.0%
!! CAGGAAATTTTGAGAGACAT+TGG + tig0022357:63094-63113 Msa1399420:CDS 35.0%
!! GGAAATTTTGAGAGACATTG+GGG + tig0022357:63096-63115 Msa1399420:CDS 35.0%
GAAACATGCATGGGAAATTT+AGG - tig0022357:63144-63163 None:intergenic 35.0%
! GGATTAAACATGCACCATTT+TGG - tig0022357:63225-63244 None:intergenic 35.0%
AAGATGATCCATATCCAAGT+GGG - tig0022357:63314-63333 None:intergenic 35.0%
TGGATCATCTTGCAACAATT+TGG + tig0022357:63323-63342 Msa1399420:CDS 35.0%
TGCAACAATTTGGATGCTAA+TGG + tig0022357:63333-63352 Msa1399420:CDS 35.0%
!!! ACTTGGCTTGCAATTTTGAA+GGG + tig0022357:63406-63425 Msa1399420:CDS 35.0%
ACAAAGAAGAGGATGAAGAA+GGG - tig0022357:63494-63513 None:intergenic 35.0%
AACAAAGAAGAGGATGAAGA+AGG - tig0022357:63495-63514 None:intergenic 35.0%
AAATGAAGGGAACAAAGAAG+AGG - tig0022357:63505-63524 None:intergenic 35.0%
ATGTGGTGTGAAAATAAGGG+AGG - tig0022357:60024-60043 None:intergenic 40.0%
AGAAGCTAGGAAAATGGAGT+GGG - tig0022357:60275-60294 None:intergenic 40.0%
AAGAAGCTAGGAAAATGGAG+TGG - tig0022357:60276-60295 None:intergenic 40.0%
! TTTTGGTATGTGGAAGGACT+TGG + tig0022357:60623-60642 Msa1399420:CDS 40.0%
GAAGAAACTATGAGTGCACT+TGG + tig0022357:60749-60768 Msa1399420:CDS 40.0%
AAACTATGAGTGCACTTGGT+GGG + tig0022357:60753-60772 Msa1399420:CDS 40.0%
!! TGGGTCAGGTATTGAAGTAA+TGG + tig0022357:60772-60791 Msa1399420:CDS 40.0%
!! TTGAAGTTCTGGAAGTACCA+GGG - tig0022357:61138-61157 None:intergenic 40.0%
!! TTTGAAGTTCTGGAAGTACC+AGG - tig0022357:61139-61158 None:intergenic 40.0%
GTTATTCGATCTTGATCGGA+CGG - tig0022357:61438-61457 None:intergenic 40.0%
! GCTTTTTGTTCTGACATGGA+CGG + tig0022357:61684-61703 Msa1399420:intron 40.0%
! TTCCCATTTTGGATTGTACC+TGG + tig0022357:62168-62187 Msa1399420:intron 40.0%
! TCCCATTTTGGATTGTACCT+GGG + tig0022357:62169-62188 Msa1399420:intron 40.0%
ACCCAGGTACAATCCAAAAT+GGG - tig0022357:62173-62192 None:intergenic 40.0%
! ATTTTGGATTGTACCTGGGT+CGG + tig0022357:62173-62192 Msa1399420:intron 40.0%
CAAAAAATATGCAGGAGCTG+AGG - tig0022357:62295-62314 None:intergenic 40.0%
TGTTACAGGTATGTAGCAGT+TGG + tig0022357:62451-62470 Msa1399420:intron 40.0%
!! GCCCTAAATCAAGTTGGATT+AGG + tig0022357:62550-62569 Msa1399420:CDS 40.0%
!! ATCAGCATTTAAGGGTACTG+TGG - tig0022357:62590-62609 None:intergenic 40.0%
GGAACAGCATTGTTTGAATC+TGG - tig0022357:62625-62644 None:intergenic 40.0%
! GCATTTTTGTCACCATCTGT+GGG - tig0022357:62937-62956 None:intergenic 40.0%
! TGCATTTTTGTCACCATCTG+TGG - tig0022357:62938-62957 None:intergenic 40.0%
! CCTTTATTTGATGCAAGCCT+AGG - tig0022357:63000-63019 None:intergenic 40.0%
CCTAGGCTTGCATCAAATAA+AGG + tig0022357:62997-63016 Msa1399420:CDS 40.0%
! AAAGGTACACCAAGAAGACA+AGG + tig0022357:63015-63034 Msa1399420:CDS 40.0%
CTCAAAATTTCCTGGCTCAA+TGG - tig0022357:63088-63107 None:intergenic 40.0%
AGATGATCCATATCCAAGTG+GGG - tig0022357:63313-63332 None:intergenic 40.0%
CAAGATGATCCATATCCAAG+TGG - tig0022357:63315-63334 None:intergenic 40.0%
AGTGCAAGATCAGAATGACT+TGG + tig0022357:63389-63408 Msa1399420:CDS 40.0%
!!! GACTTGGCTTGCAATTTTGA+AGG + tig0022357:63405-63424 Msa1399420:CDS 40.0%
TGAGATCTTGTACTTGGAGA+TGG + tig0022357:63637-63656 Msa1399420:three_prime_UTR 40.0%
!! TGGAAGGACTTGGTGTGAAT+TGG + tig0022357:60633-60652 Msa1399420:CDS 45.0%
!! GGAAGGACTTGGTGTGAATT+GGG + tig0022357:60634-60653 Msa1399420:CDS 45.0%
!! GAAGGACTTGGTGTGAATTG+GGG + tig0022357:60635-60654 Msa1399420:CDS 45.0%
GAAACTATGAGTGCACTTGG+TGG + tig0022357:60752-60771 Msa1399420:CDS 45.0%
! ATCGGACGGTTGATATCATG+TGG - tig0022357:61424-61443 None:intergenic 45.0%
AACCCAACAAACCTGCATAC+AGG - tig0022357:62079-62098 None:intergenic 45.0%
! TAGTTTAGGGCTACACCGTT+TGG + tig0022357:62126-62145 Msa1399420:intron 45.0%
GACCCAGGTACAATCCAAAA+TGG - tig0022357:62174-62193 None:intergenic 45.0%
! TTTTGGATTGTACCTGGGTC+GGG + tig0022357:62174-62193 Msa1399420:intron 45.0%
AGAGTACAGGGAGAGAGATT+GGG - tig0022357:62211-62230 None:intergenic 45.0%
! AAGAGTACAGGGAGAGAGAT+TGG - tig0022357:62212-62231 None:intergenic 45.0%
AATGTTCATTAGGGCAGGGA+AGG - tig0022357:62524-62543 None:intergenic 45.0%
AACAATGCTGTTCCATCAGC+TGG + tig0022357:62631-62650 Msa1399420:CDS 45.0%
GGCCTAAATATTCCAGCTGA+TGG - tig0022357:62646-62665 None:intergenic 45.0%
GGGTAAATGTTGACCGTGAA+TGG - tig0022357:62724-62743 None:intergenic 45.0%
GATGCAAAATCCATTGAGCC+AGG + tig0022357:63075-63094 Msa1399420:CDS 45.0%
TCTTGTACTTGGAGATGGTG+TGG + tig0022357:63642-63661 Msa1399420:three_prime_UTR 45.0%
!!! AAAAGTTTTAAAAATATATT+TGG - tig0022357:60501-60520 None:intergenic 5.0%
!!! TTATTTATTATATTTTTCTT+TGG + tig0022357:62238-62257 Msa1399420:intron 5.0%
ACTTCCGACCTTCTCGATCA+CGG - tig0022357:61098-61117 None:intergenic 50.0%
AGAAGGTCGGAAGTCGTGAT+CGG + tig0022357:61104-61123 Msa1399420:intron 50.0%
! CGCCTGTATGCAGGTTTGTT+GGG + tig0022357:62074-62093 Msa1399420:intron 50.0%
AGTACAGGGAGAGAGATTGG+GGG - tig0022357:62209-62228 None:intergenic 50.0%
GAGTACAGGGAGAGAGATTG+GGG - tig0022357:62210-62229 None:intergenic 50.0%
! TTTGCTGATTGCACCCCACT+TGG + tig0022357:63297-63316 Msa1399420:CDS 50.0%
GATTGCACCCCACTTGGATA+TGG + tig0022357:63303-63322 Msa1399420:CDS 50.0%
! ATGAGTGCACTTGGTGGGTC+AGG + tig0022357:60758-60777 Msa1399420:CDS 55.0%
GAAGCCGTGATCGAGAAGGT+CGG + tig0022357:61091-61110 Msa1399420:intron 55.0%
GAAGGTCGGAAGTCGTGATC+GGG + tig0022357:61105-61124 Msa1399420:intron 55.0%
AAGGTCGGAAGTCGTGATCG+GGG + tig0022357:61106-61125 Msa1399420:intron 55.0%
GCGCCTGTATGCAGGTTTGT+TGG + tig0022357:62073-62092 Msa1399420:intron 55.0%
GTACAGGGAGAGAGATTGGG+GGG - tig0022357:62208-62227 None:intergenic 55.0%
! GAAGTAGGTTGGCCCACAGA+TGG + tig0022357:62922-62941 Msa1399420:CDS 55.0%
GTCGGAAGCCGTGATCGAGA+AGG + tig0022357:61087-61106 Msa1399420:intron 60.0%
CGTGATCGGGGTTTGAACCC+TGG + tig0022357:61118-61137 Msa1399420:intron 60.0%
CTGGTTTCGCGCCTGTATGC+AGG + tig0022357:62065-62084 Msa1399420:intron 60.0%
GGGGACATTAGACCCGACCC+AGG - tig0022357:62189-62208 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0022357 gene 59886 63733 59886 ID=Msa1399420;Name=Msa1399420
tig0022357 mRNA 59886 63733 59886 ID=Msa1399420-mRNA-1;Parent=Msa1399420;Name=Msa1399420-mRNA-1;_AED=0.00;_eAED=0.00;_QI=680|1|1|1|1|1|2|161|484
tig0022357 exon 59886 60906 59886 ID=Msa1399420-mRNA-1:exon:17181;Parent=Msa1399420-mRNA-1
tig0022357 exon 62459 63733 62459 ID=Msa1399420-mRNA-1:exon:17182;Parent=Msa1399420-mRNA-1
tig0022357 five_prime_UTR 59886 60565 59886 ID=Msa1399420-mRNA-1:five_prime_utr;Parent=Msa1399420-mRNA-1
tig0022357 CDS 60566 60906 60566 ID=Msa1399420-mRNA-1:cds;Parent=Msa1399420-mRNA-1
tig0022357 CDS 62459 63572 62459 ID=Msa1399420-mRNA-1:cds;Parent=Msa1399420-mRNA-1
tig0022357 three_prime_UTR 63573 63733 63573 ID=Msa1399420-mRNA-1:three_prime_utr;Parent=Msa1399420-mRNA-1
Gene Sequence

>Msa1399420

ATGGGGAAAAATTGGTTTTCAAGATGGGTTTTTATGTTTATGATGATGAGTACAATTTTTTGGTATGTGGAAGGACTTGGTGTGAATTGGGGAACACAAGCAACACATCCATTGAAACCAGATACAGTTGTACAAATGTTGAAAGATAATGGAATTGAAAAAGTGAAGTTATTTGATGCTGATGAAGAAACTATGAGTGCACTTGGTGGGTCAGGTATTGAAGTAATGGTTGCTATTCCTAATAATCAATTAGCTGAAATGAGTTATTATGATCGTGCTTTGCAATGGGTTAGAAAGAATGTTACTCGTTATAACTTCAAATCTGGTGGAGTAAATATCAAGTATGTAGCAGTTGGAAATGAGCCATTTTTGAAATCATACAACAATTCATTCTTGAATGTAACCTTCCCTGCCCTAATGAACATTCAAAATGCCCTAAATCAAGTTGGATTAGGTGACTCAATTAAGGCCACAGTACCCTTAAATGCTGATGTTTATGAATCTCCAGATTCAAACAATGCTGTTCCATCAGCTGGAATATTTAGGCCTGATTTAAGTGAACTTATGACACAAATAGTCCAATTTTTGAGCAAAAATAATGCACCATTCACGGTCAACATTTACCCTTTTTTAAGTCTTTATGGAAATGACAATTTTCCATTTGACTTTGCATTTTTTGATGGAGTATCAAACCCTATAAATGACAATGGAATTTTGTACACAAATGTTTTTGATGCAAATTTTGATACTTTAGTCTCAGCATTGAAATCAATTGGATTTGGTAACATTCCAATTTTTGTTGGAGAAGTAGGTTGGCCCACAGATGGTGACAAAAATGCAAATATTCAAAATGCATTAAGATTCTATAATGGTCTTTTACCTAGGCTTGCATCAAATAAAGGTACACCAAGAAGACAAGGTTACATTGAAGTTTATTTATTTGGATTAATTGATGAAGATGCAAAATCCATTGAGCCAGGAAATTTTGAGAGACATTGGGGAATTTTTAGTTTTGATGGTAAACCTAAATTTCCCATGCATGTTTCAAATCAACATGGTCAAAATAAATTATTGGTTGGTGCAAAAGATGTGCATTATCTTGATCCAAAATGGTGCATGTTTAATCCAAATGCCAATAATTTAAGCAACCTTGCAGATAATATTAATTATGCTTGCAGTTTTGCTGATTGCACCCCACTTGGATATGGATCATCTTGCAACAATTTGGATGCTAATGGAAATGCTTCATATGCATTTAATATGTATTATCAAGTGCAAGATCAGAATGACTTGGCTTGCAATTTTGAAGGGTTAGCTATGATTACAACAAATAATATTTCAACTCCTACTTGTGATTTTATTATTCAAATTAACCCTTCTTCATCCTCTTCTTTGTTCCCTTCATTTGTTGCTTTCTTGTTTTTTAGTATACTACCTTTGATTCTATTGTGTTAG
Protein sequence

>Msa1399420

MGKNWFSRWVFMFMMMSTIFWYVEGLGVNWGTQATHPLKPDTVVQMLKDNGIEKVKLFDADEETMSALGGSGIEVMVAIPNNQLAEMSYYDRALQWVRKNVTRYNFKSGGVNIKYVAVGNEPFLKSYNNSFLNVTFPALMNIQNALNQVGLGDSIKATVPLNADVYESPDSNNAVPSAGIFRPDLSELMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDFAFFDGVSNPINDNGILYTNVFDANFDTLVSALKSIGFGNIPIFVGEVGWPTDGDKNANIQNALRFYNGLLPRLASNKGTPRRQGYIEVYLFGLIDEDAKSIEPGNFERHWGIFSFDGKPKFPMHVSNQHGQNKLLVGAKDVHYLDPKWCMFNPNANNLSNLADNINYACSFADCTPLGYGSSCNNLDANGNASYAFNMYYQVQDQNDLACNFEGLAMITTNNISTPTCDFIIQINPSSSSSLFPSFVAFLFFSILPLILLC*