AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0981280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0981280 MtrunA17_Chr6g0453551 84.500 200 11 1 1 180 117 316 2.65e-124 352
Msa0981280 MtrunA17_Chr7g0217941 73.000 200 34 1 1 180 117 316 9.62e-110 315
Msa0981280 MtrunA17_Chr6g0487101 49.432 176 70 2 4 160 200 375 2.69e-52 171
Msa0981280 MtrunA17_Chr8g0389771 46.591 176 75 2 4 160 197 372 3.57e-50 166
Msa0981280 MtrunA17_Chr1g0194311 44.318 176 79 3 4 160 188 363 3.69e-42 144
Msa0981280 MtrunA17_Chr4g0070051 40.110 182 80 4 3 162 166 340 2.84e-36 129
Msa0981280 MtrunA17_Chr7g0265201 39.560 182 85 3 3 160 200 380 1.38e-35 127
Msa0981280 MtrunA17_Chr7g0258341 36.757 185 90 2 3 160 198 382 2.60e-35 127
Msa0981280 MtrunA17_Chr5g0446301 37.989 179 86 2 4 160 177 352 9.29e-34 122
Msa0981280 MtrunA17_Chr5g0399221 35.450 189 93 4 4 169 178 360 5.41e-33 120
Msa0981280 MtrunA17_Chr8g0351871 36.216 185 89 3 5 162 137 319 1.26e-32 119
Msa0981280 MtrunA17_Chr6g0466961 38.202 178 90 3 4 162 145 321 2.53e-32 118
Msa0981280 MtrunA17_Chr8g0345281 33.684 190 92 3 4 159 383 572 6.26e-31 117
Msa0981280 MtrunA17_Chr1g0192311 34.597 211 96 8 4 178 362 566 1.43e-30 116
Msa0981280 MtrunA17_Chr1g0192281 34.597 211 96 8 4 178 362 566 1.43e-30 116
Msa0981280 MtrunA17_Chr6g0466951 37.853 177 90 3 5 162 175 350 2.86e-30 113
Msa0981280 MtrunA17_Chr1g0192321 33.962 212 99 7 4 180 376 581 3.47e-30 115
Msa0981280 MtrunA17_Chr4g0018371 36.257 171 77 3 3 141 331 501 4.12e-30 115
Msa0981280 MtrunA17_Chr7g0269541 34.211 190 91 4 4 159 350 539 7.70e-30 114
Msa0981280 MtrunA17_Chr7g0234711 31.455 213 105 7 3 180 61 267 9.18e-30 110
Msa0981280 MtrunA17_Chr4g0000121 33.649 211 98 7 4 178 349 553 1.18e-29 114
Msa0981280 MtrunA17_Chr3g0080381 31.100 209 105 5 4 179 423 625 2.42e-29 113
Msa0981280 MtrunA17_Chr8g0345211 34.343 198 93 5 4 168 378 571 2.66e-29 112
Msa0981280 MtrunA17_Chr2g0314691 33.520 179 94 3 4 159 19 195 3.61e-29 107
Msa0981280 MtrunA17_Chr3g0079351 32.394 213 103 8 3 180 319 525 7.15e-29 111
Msa0981280 MtrunA17_Chr5g0420831 33.333 180 95 3 3 159 178 355 1.04e-28 109
Msa0981280 MtrunA17_Chr7g0269511 33.333 201 96 6 4 170 359 555 1.10e-28 111
Msa0981280 MtrunA17_Chr2g0302001 32.275 189 95 3 4 159 232 420 1.44e-28 109
Msa0981280 MtrunA17_Chr1g0192301 33.333 213 96 8 4 178 367 571 1.47e-28 110
Msa0981280 MtrunA17_Chr1g0195301 31.905 210 104 5 4 180 350 553 1.52e-28 110
Msa0981280 MtrunA17_Chr1g0195321 31.905 210 104 5 4 180 350 553 1.52e-28 110
Msa0981280 MtrunA17_Chr7g0272141 34.857 175 90 3 4 154 345 519 1.57e-28 110
Msa0981280 MtrunA17_Chr3g0079361 31.925 213 104 8 3 180 319 525 2.52e-28 110
Msa0981280 MtrunA17_Chr1g0195311 31.905 210 104 5 4 180 109 312 2.92e-28 107
Msa0981280 MtrunA17_Chr7g0269521 32.500 200 99 4 4 170 357 553 3.27e-28 109
Msa0981280 MtrunA17_Chr3g0090091 31.455 213 105 8 3 180 320 526 4.60e-28 109
Msa0981280 MtrunA17_Chr1g0147051 32.105 190 95 4 4 159 401 590 5.44e-28 109
Msa0981280 MtrunA17_Chr4g0037751 32.778 180 96 3 3 159 178 355 6.37e-28 107
Msa0981280 MtrunA17_Chr8g0354911 31.579 209 104 5 4 179 407 609 6.76e-28 108
Msa0981280 MtrunA17_Chr7g0272151 33.019 212 100 8 4 179 375 580 9.00e-28 108
Msa0981280 MtrunA17_Chr3g0126771 31.053 190 97 4 4 159 391 580 1.08e-27 108
Msa0981280 MtrunA17_Chr2g0295551 30.332 211 109 6 4 179 354 561 1.21e-27 108
Msa0981280 MtrunA17_Chr3g0079341 31.455 213 105 8 3 180 319 525 1.27e-27 107
Msa0981280 MtrunA17_Chr5g0440871 31.606 193 101 3 3 170 134 320 1.41e-27 105
Msa0981280 MtrunA17_Chr1g0195331 31.429 210 105 5 4 180 350 553 1.95e-27 107
Msa0981280 MtrunA17_Chr1g0192331 32.812 192 93 3 4 159 320 511 3.96e-27 106
Msa0981280 MtrunA17_Chr4g0069861 33.684 190 92 5 4 159 311 500 4.31e-27 106
Msa0981280 MtrunA17_Chr4g0069881 31.937 191 95 4 4 159 328 518 5.13e-27 106
Msa0981280 MtrunA17_Chr4g0069841 33.508 191 93 5 4 160 310 500 6.13e-27 105
Msa0981280 MtrunA17_Chr1g0175661 30.288 208 106 5 4 178 353 554 6.38e-27 106
Msa0981280 MtrunA17_Chr1g0161731 34.615 182 95 2 5 162 155 336 7.94e-27 103
Msa0981280 MtrunA17_Chr3g0080391 32.796 186 94 4 4 159 373 557 1.24e-26 105
Msa0981280 MtrunA17_Chr7g0269531 30.769 208 105 5 4 178 360 561 2.34e-26 104
Msa0981280 MtrunA17_Chr2g0302021 30.890 191 98 4 4 160 319 509 2.34e-26 104
Msa0981280 MtrunA17_Chr1g0195351 30.952 210 104 6 4 180 350 551 5.18e-26 103
Msa0981280 MtrunA17_Chr2g0295531 30.806 211 108 5 4 179 336 543 5.31e-26 103
Msa0981280 MtrunA17_Chr2g0294321 34.286 175 91 3 18 168 176 350 7.09e-26 101
Msa0981280 MtrunA17_Chr4g0011771 33.862 189 92 3 4 159 308 496 1.21e-25 102
Msa0981280 MtrunA17_Chr4g0011791 34.225 187 92 4 4 159 306 492 1.47e-25 102
Msa0981280 MtrunA17_Chr4g0069891 30.688 189 98 3 4 159 389 577 2.20e-25 102
Msa0981280 MtrunA17_Chr5g0437831 30.890 191 98 4 3 159 176 366 2.68e-25 100
Msa0981280 MtrunA17_Chr8g0354901 30.108 186 101 3 4 160 380 565 4.47e-25 100
Msa0981280 MtrunA17_Chr7g0270691 33.673 196 97 4 4 166 344 539 8.15e-25 100
Msa0981280 MtrunA17_Chr4g0067601 28.436 211 113 5 4 179 362 569 1.09e-24 99.8
Msa0981280 MtrunA17_Chr4g0011751 31.606 193 98 4 4 162 283 475 1.44e-24 99.4
Msa0981280 MtrunA17_Chr4g0067611 27.962 211 114 5 4 179 362 569 2.40e-24 99.0
Msa0981280 MtrunA17_Chr7g0233801 30.366 191 98 4 4 159 346 536 7.80e-24 97.4
Msa0981280 MtrunA17_Chr8g0380581 29.319 191 102 4 2 159 169 359 9.45e-24 95.9
Msa0981280 MtrunA17_Chr2g0301931 30.481 187 100 3 4 160 91 277 1.44e-23 94.4
Msa0981280 MtrunA17_Chr5g0440901 36.090 133 63 2 2 112 10 142 1.62e-23 90.9
Msa0981280 MtrunA17_Chr8g0384121 31.282 195 95 2 4 159 353 547 2.03e-23 96.3
Msa0981280 MtrunA17_Chr1g0194091 29.439 214 109 6 4 179 357 566 2.19e-23 96.3
Msa0981280 MtrunA17_Chr7g0270651 29.353 201 106 4 4 168 363 563 4.24e-23 95.5
Msa0981280 MtrunA17_Chr7g0270631 29.353 201 106 4 4 168 351 551 4.38e-23 95.5
Msa0981280 MtrunA17_Chr7g0270631 26.906 223 112 6 4 178 899 1118 4.49e-20 86.7
Msa0981280 MtrunA17_Chr8g0391291 31.579 190 96 4 4 159 333 522 1.08e-22 94.0
Msa0981280 MtrunA17_Chr8g0391311 31.579 190 96 4 4 159 335 524 1.22e-22 94.0
Msa0981280 MtrunA17_Chr3g0129921 30.256 195 97 2 4 159 353 547 5.78e-22 92.0
Msa0981280 MtrunA17_Chr7g0233831 30.409 171 86 3 4 141 347 517 1.07e-21 91.3
Msa0981280 MtrunA17_Chr7g0233771 30.729 192 97 5 4 159 346 537 1.88e-21 90.5
Msa0981280 MtrunA17_Chr7g0265161 32.738 168 93 5 4 160 49 207 4.19e-21 86.7
Msa0981280 MtrunA17_Chr1g0194081 28.042 189 104 3 4 160 359 547 6.93e-21 89.0
Msa0981280 MtrunA17_Chr2g0295851 33.333 183 89 7 10 168 163 336 8.34e-21 87.4
Msa0981280 MtrunA17_Chr7g0270661 29.208 202 105 6 4 168 363 563 1.71e-20 87.8
Msa0981280 MtrunA17_Chr7g0270671 29.208 202 105 6 4 168 363 563 1.71e-20 87.8
Msa0981280 MtrunA17_Chr4g0025841 36.090 133 63 3 2 112 131 263 2.00e-20 85.5
Msa0981280 MtrunA17_Chr7g0268241 27.619 210 114 8 2 180 146 348 3.05e-19 83.6
Msa0981280 MtrunA17_Chr6g0460141 62.500 56 21 0 4 59 89 144 1.23e-18 78.2
Msa0981280 MtrunA17_Chr2g0295541 24.793 242 113 7 4 179 354 592 7.10e-18 80.5
Msa0981280 MtrunA17_Chr2g0334111 27.461 193 108 5 5 166 122 313 7.19e-18 79.3
Msa0981280 MtrunA17_Chr6g0459781 56.897 58 25 0 4 61 125 182 6.35e-17 75.1
Msa0981280 MtrunA17_Chr5g0437821 29.104 134 63 1 13 114 4 137 1.81e-15 69.7
Msa0981280 MtrunA17_Chr6g0459791 39.286 84 50 1 77 159 27 110 5.94e-15 67.8
Msa0981280 MtrunA17_Chr5g0440891 50.000 58 29 0 2 59 143 200 1.01e-12 63.5
Msa0981280 MtrunA17_Chr4g0073911 38.356 73 43 1 4 74 114 186 1.94e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0981280 AT3G29090.1 69.000 200 42 1 1 180 118 317 5.23e-102 296
Msa0981280 AT5G19730.1 49.432 176 70 2 4 160 201 376 4.65e-51 168
Msa0981280 AT2G21610.2 39.683 189 86 3 2 168 155 337 1.36e-43 148
Msa0981280 AT2G21610.1 40.000 180 83 2 2 159 150 326 1.47e-43 148
Msa0981280 AT2G36700.1 41.436 181 83 3 4 162 151 330 7.02e-42 143
Msa0981280 AT2G36710.1 40.212 189 86 3 4 168 200 385 3.05e-39 138
Msa0981280 AT5G47500.1 36.649 191 92 4 4 171 176 360 6.65e-36 128
Msa0981280 AT1G05310.1 37.297 185 89 2 3 160 199 383 3.67e-35 127
Msa0981280 AT3G17060.1 35.938 192 99 3 4 171 152 343 4.97e-33 120
Msa0981280 AT5G07430.1 36.872 179 89 2 3 159 173 349 2.25e-32 119
Msa0981280 AT5G49180.1 35.979 189 88 3 4 159 365 553 5.55e-32 120
Msa0981280 AT5G26810.1 35.938 192 94 4 5 170 147 335 6.25e-32 117
Msa0981280 AT5G07420.1 36.313 179 90 2 3 159 173 349 9.35e-32 117
Msa0981280 AT1G69940.1 34.831 178 92 2 4 159 174 349 1.15e-31 117
Msa0981280 AT4G00190.1 36.170 188 89 4 4 160 271 458 1.16e-31 119
Msa0981280 AT3G10720.1 32.500 200 99 4 4 170 54 250 6.12e-31 113
Msa0981280 AT3G05620.1 36.464 181 91 3 4 160 345 525 6.82e-31 117
Msa0981280 AT5G07410.1 34.831 178 92 2 4 159 174 349 9.21e-31 115
Msa0981280 AT5G04970.1 32.500 200 99 4 4 170 415 611 1.40e-30 117
Msa0981280 AT1G23200.1 36.813 182 90 4 4 160 357 538 6.01e-30 115
Msa0981280 AT5G55590.1 35.955 178 89 2 4 159 198 372 6.45e-30 113
Msa0981280 AT2G45220.1 35.979 189 88 4 4 159 307 495 9.42e-30 114
Msa0981280 AT3G06830.1 33.010 206 100 4 3 170 362 567 9.59e-30 114
Msa0981280 AT1G11580.1 36.649 191 85 6 4 159 351 540 1.28e-29 114
Msa0981280 AT1G11580.2 36.649 191 85 6 4 159 372 561 1.39e-29 114
Msa0981280 AT3G10720.2 32.500 200 99 4 4 170 410 606 2.09e-29 114
Msa0981280 AT4G33230.1 34.197 193 93 4 4 162 401 593 2.53e-29 113
Msa0981280 AT4G03930.1 32.857 210 103 6 3 180 319 522 5.08e-29 112
Msa0981280 AT3G27980.1 32.857 210 103 6 3 180 292 495 5.88e-29 112
Msa0981280 AT3G05610.1 32.967 182 90 3 4 154 360 540 7.79e-29 112
Msa0981280 AT1G11590.1 32.381 210 104 6 3 180 319 522 9.24e-29 111
Msa0981280 AT3G10710.1 35.484 186 91 3 4 160 360 545 1.28e-28 111
Msa0981280 AT5G61680.1 34.091 176 96 2 4 159 177 352 1.35e-28 109
Msa0981280 AT3G49220.3 31.579 190 96 3 4 159 435 624 4.92e-28 110
Msa0981280 AT3G49220.1 31.579 190 96 3 4 159 390 579 5.11e-28 109
Msa0981280 AT3G62170.1 31.414 191 98 3 4 161 383 573 6.76e-28 109
Msa0981280 AT3G14300.1 33.690 187 93 4 4 159 765 951 7.70e-28 109
Msa0981280 AT5G27870.1 31.285 179 92 2 4 151 357 535 1.73e-27 108
Msa0981280 AT2G43050.1 35.326 184 94 3 2 160 317 500 1.86e-27 107
Msa0981280 AT2G47030.1 31.414 191 98 3 4 161 383 573 2.19e-27 107
Msa0981280 AT2G47550.1 32.338 201 100 5 3 170 352 549 2.33e-27 107
Msa0981280 AT3G47400.1 35.465 172 78 4 3 141 385 556 2.59e-27 107
Msa0981280 AT3G14310.1 32.105 190 95 3 4 159 386 575 4.04e-27 107
Msa0981280 AT1G53840.1 33.333 186 94 5 4 159 385 570 4.23e-27 107
Msa0981280 AT3G43270.1 33.158 190 93 3 4 159 319 508 6.87e-27 106
Msa0981280 AT3G59010.1 33.880 183 97 3 2 160 330 512 1.33e-26 105
Msa0981280 AT5G51490.1 34.197 193 91 5 3 159 327 519 1.87e-26 105
Msa0981280 AT4G02330.1 31.217 189 97 4 4 159 366 554 2.27e-26 105
Msa0981280 AT1G53830.1 32.105 190 95 3 4 159 381 570 3.11e-26 104
Msa0981280 AT3G60730.1 31.754 211 104 5 4 180 313 517 4.21e-26 103
Msa0981280 AT5G04960.1 33.155 187 95 3 4 160 362 548 5.01e-26 103
Msa0981280 AT2G26450.1 30.000 200 102 5 4 169 406 601 6.92e-26 103
Msa0981280 AT5G64640.1 34.673 199 90 4 4 162 397 595 7.01e-26 103
Msa0981280 AT4G02320.1 30.000 210 107 5 4 179 312 515 1.32e-25 102
Msa0981280 AT2G47040.1 29.319 191 102 3 4 161 390 580 1.49e-25 102
Msa0981280 AT1G11370.1 35.507 138 58 1 4 110 150 287 1.63e-25 99.8
Msa0981280 AT4G02300.1 33.161 193 93 5 3 160 325 516 1.75e-25 102
Msa0981280 AT2G26440.1 31.746 189 96 3 4 159 342 530 3.33e-25 101
Msa0981280 AT3G24130.1 33.333 180 96 3 5 160 144 323 6.12e-25 99.0
Msa0981280 AT4G33220.2 31.579 190 96 4 4 159 196 385 6.76e-25 99.8
Msa0981280 AT4G15980.1 30.952 210 106 7 4 180 497 700 1.07e-24 100
Msa0981280 AT4G15980.2 30.952 210 106 7 4 180 507 710 1.09e-24 100
Msa0981280 AT5G51500.1 32.124 193 95 5 3 159 331 523 1.26e-24 100
Msa0981280 AT4G33220.1 31.579 190 96 4 4 159 317 506 1.32e-24 99.8
Msa0981280 AT1G02810.1 31.148 183 93 4 4 153 372 554 1.93e-24 99.8
Msa0981280 AT2G47280.1 30.928 194 100 3 5 170 142 329 2.83e-24 97.4
Msa0981280 AT2G19150.1 32.653 196 103 4 5 175 147 338 4.45e-24 96.7
Msa0981280 AT5G53370.1 32.105 190 95 3 4 159 379 568 5.16e-24 98.2
Msa0981280 AT5G20860.2 31.606 193 96 4 4 160 330 522 5.76e-24 98.2
Msa0981280 AT5G53370.2 32.105 190 95 3 4 159 415 604 6.49e-24 98.2
Msa0981280 AT5G20860.1 32.558 172 82 3 4 141 302 473 1.18e-23 97.1
Msa0981280 AT5G18990.1 32.778 180 97 3 5 160 139 318 3.34e-23 94.4
Msa0981280 AT5G55590.2 35.507 138 64 2 4 119 198 332 1.08e-22 92.8
Msa0981280 AT5G09760.1 31.818 198 96 3 4 162 347 544 2.18e-22 93.6
Msa0981280 AT5G09760.2 31.818 198 96 3 4 162 373 570 2.81e-22 93.6
Msa0981280 AT2G19150.2 32.331 133 65 2 5 112 147 279 2.20e-14 69.7

Find 42 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTGCAGGTTGGAATAAT+TGG 0.140963 6_4:-20560752 Msa0981280:CDS
GGTCTTTGCATTTACTTATA+TGG 0.186939 6_4:-20560787 Msa0981280:CDS
CATAATTTCTAAGCAGAATA+TGG 0.223122 6_4:+20560372 None:intergenic
GACACACTGTATTTACATTA+TGG 0.279427 6_4:-20561362 Msa0981280:CDS
GGAAGGCCATGGGGACCTTT+TGG 0.316288 6_4:-20560816 Msa0981280:CDS
GATTGCAGGTTGGAATAATT+GGG 0.319736 6_4:-20560751 Msa0981280:CDS
GTCCCTCGAAACGGGTGAAA+TGG 0.336495 6_4:-20560500 Msa0981280:CDS
TGGCAATAGCACTGCACTCC+TGG 0.364657 6_4:-20561288 Msa0981280:CDS
TTTACAATTGCAGGTTCCTT+GGG 0.365614 6_4:-20561484 Msa0981280:CDS
AGTGGATATGACAATGTTCC+AGG 0.395814 6_4:+20561270 None:intergenic
TTGAAAGATTGTTATATTGA+AGG 0.396540 6_4:-20561329 Msa0981280:CDS
GATCATTGTATAAAGATTGC+AGG 0.399550 6_4:-20560765 Msa0981280:CDS
GGAAGTTCATATGCATATCT+TGG 0.422152 6_4:-20560837 Msa0981280:CDS
TTGCAGGTTCCTTGGGTGGC+AGG 0.427351 6_4:-20561477 Msa0981280:intron
TCCCTCGAAACGGGTGAAAT+GGG 0.435014 6_4:-20560499 Msa0981280:CDS
CATATCCACTGCAAGTCTGC+AGG 0.437941 6_4:-20561257 Msa0981280:CDS
TCTGCTTAGAAATTATGTTA+AGG 0.443185 6_4:-20560366 Msa0981280:exon
GGCCCATTTCACCCGTTTCG+AGG 0.443339 6_4:+20560497 None:intergenic
TTCTTTATTCTTCAACACTC+TGG 0.457533 6_4:+20560476 None:intergenic
ACCTTGGCTTGCTCAAAGAA+TGG 0.481631 6_4:-20560406 Msa0981280:CDS
TTGCTCAAAGAATGGACATA+AGG 0.514749 6_4:-20560398 Msa0981280:CDS
GCCCATTTCACCCGTTTCGA+GGG 0.515455 6_4:+20560498 None:intergenic
ATGCATATCTTGGAAGGCCA+TGG 0.516421 6_4:-20560827 Msa0981280:CDS
TGCATATCTTGGAAGGCCAT+GGG 0.520080 6_4:-20560826 Msa0981280:CDS
GTTGAAGAATAAAGAAGCTG+AGG 0.523702 6_4:-20560469 Msa0981280:CDS
ACAATTGCAGGTTCCTTGGG+TGG 0.537665 6_4:-20561481 Msa0981280:CDS
GCAAAGACCACTCTTCCAAA+AGG 0.543612 6_4:+20560801 None:intergenic
ATTGCAGGTTGGAATAATTG+GGG 0.548688 6_4:-20560750 Msa0981280:CDS
TTGTTATATTGAAGGAAGTG+TGG 0.550354 6_4:-20561321 Msa0981280:CDS
ATTGTATAAAGATTGCAGGT+TGG 0.558425 6_4:-20560761 Msa0981280:CDS
TCCATTCTTTGAGCAAGCCA+AGG 0.565342 6_4:+20560405 None:intergenic
TGTTAAGGACACTTGAAACA+TGG 0.586212 6_4:-20560351 Msa0981280:three_prime_UTR
ATATCCACTGCAAGTCTGCA+GGG 0.587199 6_4:-20561256 Msa0981280:intron
GATTGCAGATGTGCTATCAC+AGG 0.587245 6_4:-20560867 Msa0981280:intron
GTTCATATGCATATCTTGGA+AGG 0.587629 6_4:-20560833 Msa0981280:CDS
TGAACCCTGCAGACTTGCAG+TGG 0.596152 6_4:+20561252 None:intergenic
TGTGCTATCACAGGCAATGG+AGG 0.600519 6_4:-20560858 Msa0981280:CDS
ACTCTTCCAAAAGGTCCCCA+TGG 0.609898 6_4:+20560810 None:intergenic
AGATGTGCTATCACAGGCAA+TGG 0.612915 6_4:-20560861 Msa0981280:CDS
GCAGGGTTCAGGGCAAGCAG+TGG 0.627011 6_4:-20561537 None:intergenic
GTAAAGTTACCTGCCACCCA+AGG 0.645790 6_4:+20561468 None:intergenic
GCATATCTTGGAAGGCCATG+GGG 0.658092 6_4:-20560825 Msa0981280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTGCTTAAAAATTAC+AGG - chr6_4:20561270-20561289 Msa0981280:CDS 15.0%
!!! ATTAAAGCTTTTTAAGATTT+GGG + chr6_4:20561524-20561543 None:intergenic 15.0%
!!! TATTAAAGCTTTTTAAGATT+TGG + chr6_4:20561525-20561544 None:intergenic 15.0%
!!! TAATTTTGCTTAAAAATTAC+AGG - chr6_4:20561270-20561289 Msa0981280:CDS 15.0%
!!! ATTAAAGCTTTTTAAGATTT+GGG + chr6_4:20561524-20561543 None:intergenic 15.0%
!!! TATTAAAGCTTTTTAAGATT+TGG + chr6_4:20561525-20561544 None:intergenic 15.0%
!! TTGAAAGATTGTTATATTGA+AGG - chr6_4:20560483-20560502 Msa0981280:CDS 20.0%
!! TATAAAGTGTAAATGATTTG+TGG - chr6_4:20560919-20560938 Msa0981280:intron 20.0%
!! ATTACATGTCTCTAAATATA+AGG - chr6_4:20561154-20561173 Msa0981280:intron 20.0%
!!! TAAGATTTGGGTTTTATTTT+AGG + chr6_4:20561512-20561531 None:intergenic 20.0%
!! TTGAAAGATTGTTATATTGA+AGG - chr6_4:20560483-20560502 Msa0981280:CDS 20.0%
!! TATAAAGTGTAAATGATTTG+TGG - chr6_4:20560919-20560938 Msa0981280:intron 20.0%
!! ATTACATGTCTCTAAATATA+AGG - chr6_4:20561154-20561173 Msa0981280:intron 20.0%
!!! TAAGATTTGGGTTTTATTTT+AGG + chr6_4:20561512-20561531 None:intergenic 20.0%
!! TTTTTCATTGTTTATGATGC+AGG - chr6_4:20560428-20560447 Msa0981280:CDS 25.0%
! GCATTAACACTTTAAATTGA+AGG + chr6_4:20560834-20560853 None:intergenic 25.0%
! GAAATCTACTTATATGTTCT+AGG - chr6_4:20560863-20560882 Msa0981280:intron 25.0%
! CATTCAGTAATATTAACTAG+GGG + chr6_4:20561247-20561266 None:intergenic 25.0%
! GCATTCAGTAATATTAACTA+GGG + chr6_4:20561248-20561267 None:intergenic 25.0%
! TGCATTCAGTAATATTAACT+AGG + chr6_4:20561249-20561268 None:intergenic 25.0%
!! CTTAAAAATTACAGGTGTTT+TGG - chr6_4:20561278-20561297 Msa0981280:CDS 25.0%
! CATAATTTCTAAGCAGAATA+TGG + chr6_4:20561443-20561462 None:intergenic 25.0%
! TCTGCTTAGAAATTATGTTA+AGG - chr6_4:20561446-20561465 Msa0981280:intron 25.0%
!!! AGCTTTAATATTTTCATACC+AGG - chr6_4:20561535-20561554 Msa0981280:CDS 25.0%
!! TTTTTCATTGTTTATGATGC+AGG - chr6_4:20560428-20560447 Msa0981280:CDS 25.0%
! GCATTAACACTTTAAATTGA+AGG + chr6_4:20560834-20560853 None:intergenic 25.0%
! GAAATCTACTTATATGTTCT+AGG - chr6_4:20560863-20560882 Msa0981280:intron 25.0%
! CATTCAGTAATATTAACTAG+GGG + chr6_4:20561247-20561266 None:intergenic 25.0%
! GCATTCAGTAATATTAACTA+GGG + chr6_4:20561248-20561267 None:intergenic 25.0%
! TGCATTCAGTAATATTAACT+AGG + chr6_4:20561249-20561268 None:intergenic 25.0%
!! CTTAAAAATTACAGGTGTTT+TGG - chr6_4:20561278-20561297 Msa0981280:CDS 25.0%
! CATAATTTCTAAGCAGAATA+TGG + chr6_4:20561443-20561462 None:intergenic 25.0%
! TCTGCTTAGAAATTATGTTA+AGG - chr6_4:20561446-20561465 Msa0981280:intron 25.0%
CTCTTGATCTATTTGTTCAT+AGG - chr6_4:20560368-20560387 Msa0981280:exon 30.0%
GTTGGAACAAGAATTTGAAA+AGG - chr6_4:20560397-20560416 Msa0981280:CDS 30.0%
GACACACTGTATTTACATTA+TGG - chr6_4:20560450-20560469 Msa0981280:CDS 30.0%
TTGTTATATTGAAGGAAGTG+TGG - chr6_4:20560491-20560510 Msa0981280:CDS 30.0%
AAATCTCCACATGAAAAAAC+TGG - chr6_4:20560600-20560619 Msa0981280:intron 30.0%
!! AAACTGGTCATGTATTTTTG+AGG - chr6_4:20560616-20560635 Msa0981280:intron 30.0%
TAACACGAAATATATGCATG+TGG + chr6_4:20560775-20560794 None:intergenic 30.0%
!! CTTATATGTTCTAGGCATTT+TGG - chr6_4:20560871-20560890 Msa0981280:intron 30.0%
GGTCTTTGCATTTACTTATA+TGG - chr6_4:20561025-20561044 Msa0981280:intron 30.0%
GATCATTGTATAAAGATTGC+AGG - chr6_4:20561047-20561066 Msa0981280:intron 30.0%
ATTGTATAAAGATTGCAGGT+TGG - chr6_4:20561051-20561070 Msa0981280:intron 30.0%
GACATGTAATACAAAGATAG+AGG + chr6_4:20561144-20561163 None:intergenic 30.0%
TTCTTTATTCTTCAACACTC+TGG + chr6_4:20561339-20561358 None:intergenic 30.0%
CTCTTGATCTATTTGTTCAT+AGG - chr6_4:20560368-20560387 Msa0981280:exon 30.0%
GTTGGAACAAGAATTTGAAA+AGG - chr6_4:20560397-20560416 Msa0981280:CDS 30.0%
GACACACTGTATTTACATTA+TGG - chr6_4:20560450-20560469 Msa0981280:CDS 30.0%
TTGTTATATTGAAGGAAGTG+TGG - chr6_4:20560491-20560510 Msa0981280:CDS 30.0%
AAATCTCCACATGAAAAAAC+TGG - chr6_4:20560600-20560619 Msa0981280:intron 30.0%
!! AAACTGGTCATGTATTTTTG+AGG - chr6_4:20560616-20560635 Msa0981280:intron 30.0%
TAACACGAAATATATGCATG+TGG + chr6_4:20560775-20560794 None:intergenic 30.0%
!! CTTATATGTTCTAGGCATTT+TGG - chr6_4:20560871-20560890 Msa0981280:intron 30.0%
GGTCTTTGCATTTACTTATA+TGG - chr6_4:20561025-20561044 Msa0981280:intron 30.0%
GATCATTGTATAAAGATTGC+AGG - chr6_4:20561047-20561066 Msa0981280:intron 30.0%
ATTGTATAAAGATTGCAGGT+TGG - chr6_4:20561051-20561070 Msa0981280:intron 30.0%
GACATGTAATACAAAGATAG+AGG + chr6_4:20561144-20561163 None:intergenic 30.0%
TTCTTTATTCTTCAACACTC+TGG + chr6_4:20561339-20561358 None:intergenic 30.0%
! GATGTGCATTTTACAATTGC+AGG - chr6_4:20560319-20560338 Msa0981280:three_prime_UTR 35.0%
! TTTTACAATTGCAGGTTCCT+TGG - chr6_4:20560327-20560346 Msa0981280:three_prime_UTR 35.0%
TTTACAATTGCAGGTTCCTT+GGG - chr6_4:20560328-20560347 Msa0981280:three_prime_UTR 35.0%
TTTGTTCATAGGTTAGACGT+TGG - chr6_4:20560379-20560398 Msa0981280:exon 35.0%
!!! ACATGACCAGTTTTTTCATG+TGG + chr6_4:20560609-20560628 None:intergenic 35.0%
CCAGTTTCTCAAACTATTAC+TGG + chr6_4:20560736-20560755 None:intergenic 35.0%
CCAGTAATAGTTTGAGAAAC+TGG - chr6_4:20560733-20560752 Msa0981280:CDS 35.0%
GGAAGTTCATATGCATATCT+TGG - chr6_4:20560975-20560994 Msa0981280:intron 35.0%
GTTCATATGCATATCTTGGA+AGG - chr6_4:20560979-20560998 Msa0981280:intron 35.0%
! AGATTGCAGGTTGGAATAAT+TGG - chr6_4:20561060-20561079 Msa0981280:intron 35.0%
! GATTGCAGGTTGGAATAATT+GGG - chr6_4:20561061-20561080 Msa0981280:intron 35.0%
! ATTGCAGGTTGGAATAATTG+GGG - chr6_4:20561062-20561081 Msa0981280:intron 35.0%
!! AAATTACAGGTGTTTTGGAC+CGG - chr6_4:20561283-20561302 Msa0981280:CDS 35.0%
GTTGAAGAATAAAGAAGCTG+AGG - chr6_4:20561343-20561362 Msa0981280:CDS 35.0%
TTGCTCAAAGAATGGACATA+AGG - chr6_4:20561414-20561433 Msa0981280:intron 35.0%
TGTTAAGGACACTTGAAACA+TGG - chr6_4:20561461-20561480 Msa0981280:intron 35.0%
! GATGTGCATTTTACAATTGC+AGG - chr6_4:20560319-20560338 Msa0981280:three_prime_UTR 35.0%
! TTTTACAATTGCAGGTTCCT+TGG - chr6_4:20560327-20560346 Msa0981280:three_prime_UTR 35.0%
TTTACAATTGCAGGTTCCTT+GGG - chr6_4:20560328-20560347 Msa0981280:three_prime_UTR 35.0%
TTTGTTCATAGGTTAGACGT+TGG - chr6_4:20560379-20560398 Msa0981280:exon 35.0%
!!! ACATGACCAGTTTTTTCATG+TGG + chr6_4:20560609-20560628 None:intergenic 35.0%
CCAGTTTCTCAAACTATTAC+TGG + chr6_4:20560736-20560755 None:intergenic 35.0%
CCAGTAATAGTTTGAGAAAC+TGG - chr6_4:20560733-20560752 Msa0981280:CDS 35.0%
GGAAGTTCATATGCATATCT+TGG - chr6_4:20560975-20560994 Msa0981280:intron 35.0%
GTTCATATGCATATCTTGGA+AGG - chr6_4:20560979-20560998 Msa0981280:intron 35.0%
! AGATTGCAGGTTGGAATAAT+TGG - chr6_4:20561060-20561079 Msa0981280:intron 35.0%
! GATTGCAGGTTGGAATAATT+GGG - chr6_4:20561061-20561080 Msa0981280:intron 35.0%
! ATTGCAGGTTGGAATAATTG+GGG - chr6_4:20561062-20561081 Msa0981280:intron 35.0%
!! AAATTACAGGTGTTTTGGAC+CGG - chr6_4:20561283-20561302 Msa0981280:CDS 35.0%
GTTGAAGAATAAAGAAGCTG+AGG - chr6_4:20561343-20561362 Msa0981280:CDS 35.0%
TTGCTCAAAGAATGGACATA+AGG - chr6_4:20561414-20561433 Msa0981280:intron 35.0%
TGTTAAGGACACTTGAAACA+TGG - chr6_4:20561461-20561480 Msa0981280:intron 35.0%
!! GGAAGTGTGGACTTCATTTT+TGG - chr6_4:20560504-20560523 Msa0981280:CDS 40.0%
AGTGGATATGACAATGTTCC+AGG + chr6_4:20560545-20560564 None:intergenic 40.0%
TCAACATGTGTAGTGTCATC+TGG - chr6_4:20560643-20560662 Msa0981280:intron 40.0%
CAACATGTGTAGTGTCATCT+GGG - chr6_4:20560644-20560663 Msa0981280:intron 40.0%
TGTCAGACAGTGATACATGT+CGG - chr6_4:20560796-20560815 Msa0981280:CDS 40.0%
!! AATTACAGGTGTTTTGGACC+GGG - chr6_4:20561284-20561303 Msa0981280:CDS 40.0%
ATCCAGAACCACAAAAACCT+TGG - chr6_4:20561390-20561409 Msa0981280:intron 40.0%
!! GGAAGTGTGGACTTCATTTT+TGG - chr6_4:20560504-20560523 Msa0981280:CDS 40.0%
AGTGGATATGACAATGTTCC+AGG + chr6_4:20560545-20560564 None:intergenic 40.0%
TCAACATGTGTAGTGTCATC+TGG - chr6_4:20560643-20560662 Msa0981280:intron 40.0%
CAACATGTGTAGTGTCATCT+GGG - chr6_4:20560644-20560663 Msa0981280:intron 40.0%
TGTCAGACAGTGATACATGT+CGG - chr6_4:20560796-20560815 Msa0981280:CDS 40.0%
!! AATTACAGGTGTTTTGGACC+GGG - chr6_4:20561284-20561303 Msa0981280:CDS 40.0%
ATCCAGAACCACAAAAACCT+TGG - chr6_4:20561390-20561409 Msa0981280:intron 40.0%
ATATCCACTGCAAGTCTGCA+GGG - chr6_4:20560556-20560575 Msa0981280:intron 45.0%
GTCAGTATCATTGTCGAGTC+TGG + chr6_4:20560704-20560723 None:intergenic 45.0%
GATTGCAGATGTGCTATCAC+AGG - chr6_4:20560945-20560964 Msa0981280:intron 45.0%
AGATGTGCTATCACAGGCAA+TGG - chr6_4:20560951-20560970 Msa0981280:intron 45.0%
ATGCATATCTTGGAAGGCCA+TGG - chr6_4:20560985-20561004 Msa0981280:intron 45.0%
TGCATATCTTGGAAGGCCAT+GGG - chr6_4:20560986-20561005 Msa0981280:intron 45.0%
GCAAAGACCACTCTTCCAAA+AGG + chr6_4:20561014-20561033 None:intergenic 45.0%
! AGCCAAGGTTTTTGTGGTTC+TGG + chr6_4:20561395-20561414 None:intergenic 45.0%
! TGAGCAAGCCAAGGTTTTTG+TGG + chr6_4:20561401-20561420 None:intergenic 45.0%
!! ACCTTGGCTTGCTCAAAGAA+TGG - chr6_4:20561406-20561425 Msa0981280:intron 45.0%
TCCATTCTTTGAGCAAGCCA+AGG + chr6_4:20561410-20561429 None:intergenic 45.0%
ATATCCACTGCAAGTCTGCA+GGG - chr6_4:20560556-20560575 Msa0981280:intron 45.0%
GTCAGTATCATTGTCGAGTC+TGG + chr6_4:20560704-20560723 None:intergenic 45.0%
GATTGCAGATGTGCTATCAC+AGG - chr6_4:20560945-20560964 Msa0981280:intron 45.0%
AGATGTGCTATCACAGGCAA+TGG - chr6_4:20560951-20560970 Msa0981280:intron 45.0%
ATGCATATCTTGGAAGGCCA+TGG - chr6_4:20560985-20561004 Msa0981280:intron 45.0%
TGCATATCTTGGAAGGCCAT+GGG - chr6_4:20560986-20561005 Msa0981280:intron 45.0%
GCAAAGACCACTCTTCCAAA+AGG + chr6_4:20561014-20561033 None:intergenic 45.0%
! AGCCAAGGTTTTTGTGGTTC+TGG + chr6_4:20561395-20561414 None:intergenic 45.0%
! TGAGCAAGCCAAGGTTTTTG+TGG + chr6_4:20561401-20561420 None:intergenic 45.0%
!! ACCTTGGCTTGCTCAAAGAA+TGG - chr6_4:20561406-20561425 Msa0981280:intron 45.0%
TCCATTCTTTGAGCAAGCCA+AGG + chr6_4:20561410-20561429 None:intergenic 45.0%
ACAATTGCAGGTTCCTTGGG+TGG - chr6_4:20560331-20560350 Msa0981280:three_prime_UTR 50.0%
GTAAAGTTACCTGCCACCCA+AGG + chr6_4:20560347-20560366 None:intergenic 50.0%
CATATCCACTGCAAGTCTGC+AGG - chr6_4:20560555-20560574 Msa0981280:intron 50.0%
TGTGCTATCACAGGCAATGG+AGG - chr6_4:20560954-20560973 Msa0981280:intron 50.0%
GCATATCTTGGAAGGCCATG+GGG - chr6_4:20560987-20561006 Msa0981280:intron 50.0%
ACTCTTCCAAAAGGTCCCCA+TGG + chr6_4:20561005-20561024 None:intergenic 50.0%
!! ATGGGGACCTTTTGGAAGAG+TGG - chr6_4:20561004-20561023 Msa0981280:intron 50.0%
CGTTTCGAGGGACAAAAACC+CGG + chr6_4:20561305-20561324 None:intergenic 50.0%
! CGGGTTTTTGTCCCTCGAAA+CGG - chr6_4:20561303-20561322 Msa0981280:CDS 50.0%
! GGGTTTTTGTCCCTCGAAAC+GGG - chr6_4:20561304-20561323 Msa0981280:CDS 50.0%
TCCCTCGAAACGGGTGAAAT+GGG - chr6_4:20561313-20561332 Msa0981280:CDS 50.0%
ACAATTGCAGGTTCCTTGGG+TGG - chr6_4:20560331-20560350 Msa0981280:three_prime_UTR 50.0%
GTAAAGTTACCTGCCACCCA+AGG + chr6_4:20560347-20560366 None:intergenic 50.0%
CATATCCACTGCAAGTCTGC+AGG - chr6_4:20560555-20560574 Msa0981280:intron 50.0%
TGTGCTATCACAGGCAATGG+AGG - chr6_4:20560954-20560973 Msa0981280:intron 50.0%
GCATATCTTGGAAGGCCATG+GGG - chr6_4:20560987-20561006 Msa0981280:intron 50.0%
ACTCTTCCAAAAGGTCCCCA+TGG + chr6_4:20561005-20561024 None:intergenic 50.0%
!! ATGGGGACCTTTTGGAAGAG+TGG - chr6_4:20561004-20561023 Msa0981280:intron 50.0%
CGTTTCGAGGGACAAAAACC+CGG + chr6_4:20561305-20561324 None:intergenic 50.0%
! CGGGTTTTTGTCCCTCGAAA+CGG - chr6_4:20561303-20561322 Msa0981280:CDS 50.0%
! GGGTTTTTGTCCCTCGAAAC+GGG - chr6_4:20561304-20561323 Msa0981280:CDS 50.0%
TCCCTCGAAACGGGTGAAAT+GGG - chr6_4:20561313-20561332 Msa0981280:CDS 50.0%
TGGCAATAGCACTGCACTCC+TGG - chr6_4:20560524-20560543 Msa0981280:intron 55.0%
TGAACCCTGCAGACTTGCAG+TGG + chr6_4:20560563-20560582 None:intergenic 55.0%
GTCCCTCGAAACGGGTGAAA+TGG - chr6_4:20561312-20561331 Msa0981280:CDS 55.0%
GCCCATTTCACCCGTTTCGA+GGG + chr6_4:20561317-20561336 None:intergenic 55.0%
TGGCAATAGCACTGCACTCC+TGG - chr6_4:20560524-20560543 Msa0981280:intron 55.0%
TGAACCCTGCAGACTTGCAG+TGG + chr6_4:20560563-20560582 None:intergenic 55.0%
GTCCCTCGAAACGGGTGAAA+TGG - chr6_4:20561312-20561331 Msa0981280:CDS 55.0%
GCCCATTTCACCCGTTTCGA+GGG + chr6_4:20561317-20561336 None:intergenic 55.0%
TTGCAGGTTCCTTGGGTGGC+AGG - chr6_4:20560335-20560354 Msa0981280:three_prime_UTR 60.0%
! GGAAGGCCATGGGGACCTTT+TGG - chr6_4:20560996-20561015 Msa0981280:intron 60.0%
GGCCCATTTCACCCGTTTCG+AGG + chr6_4:20561318-20561337 None:intergenic 60.0%
TTGCAGGTTCCTTGGGTGGC+AGG - chr6_4:20560335-20560354 Msa0981280:three_prime_UTR 60.0%
! GGAAGGCCATGGGGACCTTT+TGG - chr6_4:20560996-20561015 Msa0981280:intron 60.0%
GGCCCATTTCACCCGTTTCG+AGG + chr6_4:20561318-20561337 None:intergenic 60.0%
GCCACTGCTTGCCCTGAACC+TGG + chr6_4:20560279-20560298 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 20560279 20561555 20560279 ID=Msa0981280;Name=Msa0981280
chr6_4 mRNA 20560279 20561555 20560279 ID=Msa0981280-mRNA-1;Parent=Msa0981280;Name=Msa0981280-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0.33|0.25|1|0.66|0.75|4|101|180
chr6_4 exon 20560279 20560542 20560279 ID=Msa0981280-mRNA-1:exon:6321;Parent=Msa0981280-mRNA-1
chr6_4 exon 20560708 20560881 20560708 ID=Msa0981280-mRNA-1:exon:6320;Parent=Msa0981280-mRNA-1
chr6_4 exon 20561257 20561384 20561257 ID=Msa0981280-mRNA-1:exon:6319;Parent=Msa0981280-mRNA-1
chr6_4 exon 20561478 20561555 20561478 ID=Msa0981280-mRNA-1:exon:6318;Parent=Msa0981280-mRNA-1
chr6_4 CDS 20561478 20561555 20561478 ID=Msa0981280-mRNA-1:cds;Parent=Msa0981280-mRNA-1
chr6_4 CDS 20561257 20561384 20561257 ID=Msa0981280-mRNA-1:cds;Parent=Msa0981280-mRNA-1
chr6_4 CDS 20560708 20560881 20560708 ID=Msa0981280-mRNA-1:cds;Parent=Msa0981280-mRNA-1
chr6_4 CDS 20560380 20560542 20560380 ID=Msa0981280-mRNA-1:cds;Parent=Msa0981280-mRNA-1
chr6_4 three_prime_UTR 20560279 20560379 20560279 ID=Msa0981280-mRNA-1:three_prime_utr;Parent=Msa0981280-mRNA-1
Gene Sequence

>Msa0981280

GGTTCAGGGCAAGCAGTGGCAGTTAGAGTAACAGTAGATAGATGTGCATTTTACAATTGCAGGTTCCTTGGGTGGCAGGACACACTGTATTTACATTATGGCAAACAATATTTGAAAGATTGTTATATTGAAGGAAGTGTGGACTTCATTTTTGGCAATAGCACTGCACTCCTGGAACATTGTCATATCCACTGCAAGTCTGCAGGATGTGCTATCACAGGCAATGGAGGAAGTTCATATGCATATCTTGGAAGGCCATGGGGACCTTTTGGAAGAGTGGTCTTTGCATTTACTTATATGGATCATTGTATAAAGATTGCAGGTTGGAATAATTGGGGAAAAGTTGAGAATGAAAAAAGTGCTTGTTTCTATGAATACAAGTGTTTTGGACCGGGTTTTTGTCCCTCGAAACGGGTGAAATGGGCCAGAGTGTTGAAGAATAAAGAAGCTGAGGAGTTTCTCATGCATAGTTTCATTGATCCAGAACCACAAAAACCTTGGCTTGCTCAAAGAATGGACATAAGGATTCCATATTCTGCTTAG
Protein sequence

>Msa0981280

GSGQAVAVRVTVDRCAFYNCRFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLEHCHIHCKSAGCAITGNGGSSYAYLGRPWGPFGRVVFAFTYMDHCIKIAGWNNWGKVENEKSACFYEYKCFGPGFCPSKRVKWARVLKNKEAEEFLMHSFIDPEPQKPWLAQRMDIRIPYSA*