Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0331570 | Msa0989340 | 0.865968 | 4.018916e-65 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCACCAAAAGCTTTGTTTC+CGG | 0.296395 | 6_4:-45980784 | Msa0989340:CDS |
| GATTGTGTAAGTAGGTCAAA+AGG | 0.422398 | 6_4:+45980188 | None:intergenic |
| GTGACCGGAAACAAAGCTTT+TGG | 0.424992 | 6_4:+45980780 | None:intergenic |
| CAGATCTGCTGCTAACTTCG+TGG | 0.476295 | 6_4:+45980745 | None:intergenic |
| GTTACAAAGATTGTGTAAGT+AGG | 0.481166 | 6_4:+45980180 | None:intergenic |
| ATTGTGTAAGTAGGTCAAAA+GGG | 0.521191 | 6_4:+45980189 | None:intergenic |
| GTGTGAGATCCTTGTGGATG+AGG | 0.527057 | 6_4:+45980141 | None:intergenic |
| TGGATGAGCATGAGGGTGAC+CGG | 0.527169 | 6_4:+45980765 | None:intergenic |
| AAAGGGAACTTACATGATGA+AGG | 0.546474 | 6_4:+45980206 | None:intergenic |
| AGCTCAACCATGGAGTTTAG+CGG | 0.551609 | 6_4:-45980240 | Msa0989340:CDS |
| GTCACTTTCAAGCTCAACCA+TGG | 0.563416 | 6_4:-45980250 | Msa0989340:CDS |
| AAACATTGACTTCGTTGTTG+TGG | 0.565742 | 6_4:+45980712 | None:intergenic |
| AGATCTGCCGCTAAACTCCA+TGG | 0.584643 | 6_4:+45980233 | None:intergenic |
| TTGCATGTGTGAGATCCTTG+TGG | 0.605008 | 6_4:+45980135 | None:intergenic |
| TAACTTCGTGGATGAGCATG+AGG | 0.614555 | 6_4:+45980757 | None:intergenic |
| GTGACTTATCCTCATCCACA+AGG | 0.682195 | 6_4:-45980150 | Msa0989340:three_prime_UTR |
| GTGGATGAGGATAAGTCACA+CGG | 0.691099 | 6_4:+45980154 | None:intergenic |
| AACTTCGTGGATGAGCATGA+GGG | 0.744179 | 6_4:+45980758 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATAATCTTTGATTTTT+TGG | + | chr6_4:45980795-45980814 | None:intergenic | 10.0% |
| !!! | AAAAATCAAAGATTATATTT+TGG | - | chr6_4:45980795-45980814 | Msa0989340:CDS | 10.0% |
| !! | AATATATCTGTAAAAATACA+TGG | + | chr6_4:45980470-45980489 | None:intergenic | 15.0% |
| !! | ATTATATCGTCTTAAATGTA+TGG | - | chr6_4:45980238-45980257 | Msa0989340:CDS | 20.0% |
| !!! | ATTTACCAAATTCTTTTAGT+AGG | + | chr6_4:45980373-45980392 | None:intergenic | 20.0% |
| !! | AAAAAAGGGAATCTAAAAAA+TGG | + | chr6_4:45980554-45980573 | None:intergenic | 20.0% |
| !! | AAAGGAAAACAAAAAAAAGA+GGG | + | chr6_4:45980594-45980613 | None:intergenic | 20.0% |
| ! | AATTCGTTATTCTATAGACA+CGG | - | chr6_4:45980208-45980227 | Msa0989340:CDS | 25.0% |
| !!! | TCTCTAAAATCTAAAACAGA+AGG | + | chr6_4:45980287-45980306 | None:intergenic | 25.0% |
| !!! | TCTGTTTTAGATTTTAGAGA+TGG | - | chr6_4:45980287-45980306 | Msa0989340:intron | 25.0% |
| !! | TTAGTGTAATTTTGTAGAGA+TGG | - | chr6_4:45980320-45980339 | Msa0989340:intron | 25.0% |
| !!! | CCTGTTATAATTTTTTTTGC+CGG | - | chr6_4:45980345-45980364 | Msa0989340:intron | 25.0% |
| ! | GAGAATAGGTTGTAAAAAAA+GGG | + | chr6_4:45980568-45980587 | None:intergenic | 25.0% |
| ! | AGAGAATAGGTTGTAAAAAA+AGG | + | chr6_4:45980569-45980588 | None:intergenic | 25.0% |
| ! | GAAAGGAAAACAAAAAAAAG+AGG | + | chr6_4:45980595-45980614 | None:intergenic | 25.0% |
| !! | GATTTTAGAGATGGTTTTTG+TGG | - | chr6_4:45980296-45980315 | Msa0989340:intron | 30.0% |
| CCGGCAAAAAAAATTATAAC+AGG | + | chr6_4:45980348-45980367 | None:intergenic | 30.0% | |
| ! | CAAATTCTTTTAGTAGGAAC+CGG | + | chr6_4:45980367-45980386 | None:intergenic | 30.0% |
| ATTGTGTAAGTAGGTCAAAA+GGG | + | chr6_4:45980710-45980729 | None:intergenic | 30.0% | |
| GTTACAAAGATTGTGTAAGT+AGG | + | chr6_4:45980719-45980738 | None:intergenic | 30.0% | |
| !! | AAACATTGACTTCGTTGTTG+TGG | + | chr6_4:45980187-45980206 | None:intergenic | 35.0% |
| CGGTTCCTACTAAAAGAATT+TGG | - | chr6_4:45980365-45980384 | Msa0989340:intron | 35.0% | |
| AAAAAAGAGGGAGAGAGAAT+AGG | + | chr6_4:45980582-45980601 | None:intergenic | 35.0% | |
| ! | TCCGCAAAAAGCTTCATTTT+CGG | - | chr6_4:45980624-45980643 | Msa0989340:intron | 35.0% |
| ! | ACCGAAAATGAAGCTTTTTG+CGG | + | chr6_4:45980628-45980647 | None:intergenic | 35.0% |
| AAAGGGAACTTACATGATGA+AGG | + | chr6_4:45980693-45980712 | None:intergenic | 35.0% | |
| GATTGTGTAAGTAGGTCAAA+AGG | + | chr6_4:45980711-45980730 | None:intergenic | 35.0% | |
| ! | GGAAACAAAGCTTTTGGTGG+CGG | + | chr6_4:45980113-45980132 | None:intergenic | 45.0% |
| !! | GCCACCAAAAGCTTTGTTTC+CGG | - | chr6_4:45980112-45980131 | Msa0989340:three_prime_UTR | 45.0% |
| ! | ACCGGAAACAAAGCTTTTGG+TGG | + | chr6_4:45980116-45980135 | None:intergenic | 45.0% |
| ! | GTGACCGGAAACAAAGCTTT+TGG | + | chr6_4:45980119-45980138 | None:intergenic | 45.0% |
| AACTTCGTGGATGAGCATGA+GGG | + | chr6_4:45980141-45980160 | None:intergenic | 45.0% | |
| TAACTTCGTGGATGAGCATG+AGG | + | chr6_4:45980142-45980161 | None:intergenic | 45.0% | |
| TTTGCGGAGTCGAATCTGAA+AGG | + | chr6_4:45980612-45980631 | None:intergenic | 45.0% | |
| ! | GTCACTTTCAAGCTCAACCA+TGG | - | chr6_4:45980646-45980665 | Msa0989340:intron | 45.0% |
| AGCTCAACCATGGAGTTTAG+CGG | - | chr6_4:45980656-45980675 | Msa0989340:intron | 45.0% | |
| GTGGATGAGGATAAGTCACA+CGG | + | chr6_4:45980745-45980764 | None:intergenic | 45.0% | |
| GTGACTTATCCTCATCCACA+AGG | - | chr6_4:45980746-45980765 | Msa0989340:CDS | 45.0% | |
| TTGCATGTGTGAGATCCTTG+TGG | + | chr6_4:45980764-45980783 | None:intergenic | 45.0% | |
| !!! | AAGCTTTTGGTGGCGGAGAT+CGG | + | chr6_4:45980106-45980125 | None:intergenic | 50.0% |
| CAGATCTGCTGCTAACTTCG+TGG | + | chr6_4:45980154-45980173 | None:intergenic | 50.0% | |
| AGATCTGCCGCTAAACTCCA+TGG | + | chr6_4:45980666-45980685 | None:intergenic | 50.0% | |
| GTGTGAGATCCTTGTGGATG+AGG | + | chr6_4:45980758-45980777 | None:intergenic | 50.0% | |
| TGGATGAGCATGAGGGTGAC+CGG | + | chr6_4:45980134-45980153 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 45980099 | 45980819 | 45980099 | ID=Msa0989340;Name=Msa0989340 |
| chr6_4 | mRNA | 45980099 | 45980819 | 45980099 | ID=Msa0989340-mRNA-1;Parent=Msa0989340;Name=Msa0989340-mRNA-1;_AED=0.19;_eAED=0.47;_QI=0|0|0.5|1|0|0.5|2|82|74 |
| chr6_4 | exon | 45980099 | 45980301 | 45980099 | ID=Msa0989340-mRNA-1:exon:10314;Parent=Msa0989340-mRNA-1 |
| chr6_4 | exon | 45980716 | 45980819 | 45980716 | ID=Msa0989340-mRNA-1:exon:10313;Parent=Msa0989340-mRNA-1 |
| chr6_4 | CDS | 45980716 | 45980819 | 45980716 | ID=Msa0989340-mRNA-1:cds;Parent=Msa0989340-mRNA-1 |
| chr6_4 | CDS | 45980181 | 45980301 | 45980181 | ID=Msa0989340-mRNA-1:cds;Parent=Msa0989340-mRNA-1 |
| chr6_4 | three_prime_UTR | 45980099 | 45980180 | 45980099 | ID=Msa0989340-mRNA-1:three_prime_utr;Parent=Msa0989340-mRNA-1 |
| Gene Sequence |
| Protein sequence |
