Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0331570 | Msa0989340 | 0.865968 | 4.018916e-65 | -8.615850e-47 |
| Msa0331570 | Msa1001180 | 0.801521 | 8.657409e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCACCAAAAGCTTTGTTTC+CGG | 0.296395 | 2_4:-47537319 | Msa0331570:CDS |
| GATTGTGTAAGTAGGTCAAA+AGG | 0.422398 | 2_4:+47536723 | None:intergenic |
| GTGACCGGAAACAAAGCTTT+TGG | 0.424992 | 2_4:+47537315 | None:intergenic |
| CAGATCTGCTGCTAACTTCG+TGG | 0.476295 | 2_4:+47537280 | None:intergenic |
| GTTACAAAGATTGTGTAAGT+AGG | 0.481166 | 2_4:+47536715 | None:intergenic |
| ATTGTGTAAGTAGGTCAAAA+GGG | 0.521191 | 2_4:+47536724 | None:intergenic |
| GTGTGAGATCCTTGTGGATG+AGG | 0.527057 | 2_4:+47536676 | None:intergenic |
| TGGATGAGCATGAGGGTGAC+CGG | 0.527169 | 2_4:+47537300 | None:intergenic |
| AAAGGGAACTTACATGATGA+AGG | 0.546474 | 2_4:+47536741 | None:intergenic |
| AGCTCAACCATGGAGTTTAG+CGG | 0.551609 | 2_4:-47536775 | Msa0331570:CDS |
| GTCACTTTCAAGCTCAACCA+TGG | 0.563416 | 2_4:-47536785 | Msa0331570:CDS |
| AAACATTGACTTCGTTGTTG+TGG | 0.565742 | 2_4:+47537247 | None:intergenic |
| AGATCTGCCGCTAAACTCCA+TGG | 0.584643 | 2_4:+47536768 | None:intergenic |
| TTGCATGTGTGAGATCCTTG+TGG | 0.605008 | 2_4:+47536670 | None:intergenic |
| TAACTTCGTGGATGAGCATG+AGG | 0.614555 | 2_4:+47537292 | None:intergenic |
| GTGACTTATCCTCATCCACA+AGG | 0.682195 | 2_4:-47536685 | Msa0331570:three_prime_UTR |
| GTGGATGAGGATAAGTCACA+CGG | 0.691099 | 2_4:+47536689 | None:intergenic |
| AACTTCGTGGATGAGCATGA+GGG | 0.744179 | 2_4:+47537293 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATAATCTTTGATTTTT+TGG | + | chr2_4:47537330-47537349 | None:intergenic | 10.0% |
| !!! | AAAAATCAAAGATTATATTT+TGG | - | chr2_4:47537330-47537349 | Msa0331570:CDS | 10.0% |
| !! | AATATATCTGTAAAAATACA+TGG | + | chr2_4:47537005-47537024 | None:intergenic | 15.0% |
| !! | ATTATATCGTCTTAAATGTA+TGG | - | chr2_4:47536773-47536792 | Msa0331570:CDS | 20.0% |
| !!! | ATTTACCAAATTCTTTTAGT+AGG | + | chr2_4:47536908-47536927 | None:intergenic | 20.0% |
| !! | AAAAAAGGGAATCTAAAAAA+TGG | + | chr2_4:47537089-47537108 | None:intergenic | 20.0% |
| !! | AAAGGAAAACAAAAAAAAGA+GGG | + | chr2_4:47537129-47537148 | None:intergenic | 20.0% |
| ! | AATTCGTTATTCTATAGACA+CGG | - | chr2_4:47536743-47536762 | Msa0331570:CDS | 25.0% |
| !!! | TCTCTAAAATCTAAAACAGA+AGG | + | chr2_4:47536822-47536841 | None:intergenic | 25.0% |
| !!! | TCTGTTTTAGATTTTAGAGA+TGG | - | chr2_4:47536822-47536841 | Msa0331570:intron | 25.0% |
| !! | TTAGTGTAATTTTGTAGAGA+TGG | - | chr2_4:47536855-47536874 | Msa0331570:intron | 25.0% |
| !!! | CCTGTTATAATTTTTTTTGC+CGG | - | chr2_4:47536880-47536899 | Msa0331570:intron | 25.0% |
| ! | GAGAATAGGTTGTAAAAAAA+GGG | + | chr2_4:47537103-47537122 | None:intergenic | 25.0% |
| ! | AGAGAATAGGTTGTAAAAAA+AGG | + | chr2_4:47537104-47537123 | None:intergenic | 25.0% |
| ! | GAAAGGAAAACAAAAAAAAG+AGG | + | chr2_4:47537130-47537149 | None:intergenic | 25.0% |
| !! | GATTTTAGAGATGGTTTTTG+TGG | - | chr2_4:47536831-47536850 | Msa0331570:intron | 30.0% |
| CCGGCAAAAAAAATTATAAC+AGG | + | chr2_4:47536883-47536902 | None:intergenic | 30.0% | |
| ! | CAAATTCTTTTAGTAGGAAC+CGG | + | chr2_4:47536902-47536921 | None:intergenic | 30.0% |
| ATTGTGTAAGTAGGTCAAAA+GGG | + | chr2_4:47537245-47537264 | None:intergenic | 30.0% | |
| GTTACAAAGATTGTGTAAGT+AGG | + | chr2_4:47537254-47537273 | None:intergenic | 30.0% | |
| !! | AAACATTGACTTCGTTGTTG+TGG | + | chr2_4:47536722-47536741 | None:intergenic | 35.0% |
| CGGTTCCTACTAAAAGAATT+TGG | - | chr2_4:47536900-47536919 | Msa0331570:intron | 35.0% | |
| AAAAAAGAGGGAGAGAGAAT+AGG | + | chr2_4:47537117-47537136 | None:intergenic | 35.0% | |
| ! | TCCGCAAAAAGCTTCATTTT+CGG | - | chr2_4:47537159-47537178 | Msa0331570:intron | 35.0% |
| ! | ACCGAAAATGAAGCTTTTTG+CGG | + | chr2_4:47537163-47537182 | None:intergenic | 35.0% |
| AAAGGGAACTTACATGATGA+AGG | + | chr2_4:47537228-47537247 | None:intergenic | 35.0% | |
| GATTGTGTAAGTAGGTCAAA+AGG | + | chr2_4:47537246-47537265 | None:intergenic | 35.0% | |
| ! | GGAAACAAAGCTTTTGGTGG+CGG | + | chr2_4:47536648-47536667 | None:intergenic | 45.0% |
| !! | GCCACCAAAAGCTTTGTTTC+CGG | - | chr2_4:47536647-47536666 | Msa0331570:three_prime_UTR | 45.0% |
| ! | ACCGGAAACAAAGCTTTTGG+TGG | + | chr2_4:47536651-47536670 | None:intergenic | 45.0% |
| ! | GTGACCGGAAACAAAGCTTT+TGG | + | chr2_4:47536654-47536673 | None:intergenic | 45.0% |
| AACTTCGTGGATGAGCATGA+GGG | + | chr2_4:47536676-47536695 | None:intergenic | 45.0% | |
| TAACTTCGTGGATGAGCATG+AGG | + | chr2_4:47536677-47536696 | None:intergenic | 45.0% | |
| TTTGCGGAGTCGAATCTGAA+AGG | + | chr2_4:47537147-47537166 | None:intergenic | 45.0% | |
| ! | GTCACTTTCAAGCTCAACCA+TGG | - | chr2_4:47537181-47537200 | Msa0331570:intron | 45.0% |
| AGCTCAACCATGGAGTTTAG+CGG | - | chr2_4:47537191-47537210 | Msa0331570:intron | 45.0% | |
| GTGGATGAGGATAAGTCACA+CGG | + | chr2_4:47537280-47537299 | None:intergenic | 45.0% | |
| GTGACTTATCCTCATCCACA+AGG | - | chr2_4:47537281-47537300 | Msa0331570:CDS | 45.0% | |
| TTGCATGTGTGAGATCCTTG+TGG | + | chr2_4:47537299-47537318 | None:intergenic | 45.0% | |
| !!! | AAGCTTTTGGTGGCGGAGAT+CGG | + | chr2_4:47536641-47536660 | None:intergenic | 50.0% |
| CAGATCTGCTGCTAACTTCG+TGG | + | chr2_4:47536689-47536708 | None:intergenic | 50.0% | |
| AGATCTGCCGCTAAACTCCA+TGG | + | chr2_4:47537201-47537220 | None:intergenic | 50.0% | |
| GTGTGAGATCCTTGTGGATG+AGG | + | chr2_4:47537293-47537312 | None:intergenic | 50.0% | |
| TGGATGAGCATGAGGGTGAC+CGG | + | chr2_4:47536669-47536688 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 47536634 | 47537354 | 47536634 | ID=Msa0331570;Name=Msa0331570 |
| chr2_4 | mRNA | 47536634 | 47537354 | 47536634 | ID=Msa0331570-mRNA-1;Parent=Msa0331570;Name=Msa0331570-mRNA-1;_AED=0.19;_eAED=0.47;_QI=0|0|0.5|1|0|0|2|82|74 |
| chr2_4 | exon | 47536634 | 47536836 | 47536634 | ID=Msa0331570-mRNA-1:exon:14300;Parent=Msa0331570-mRNA-1 |
| chr2_4 | exon | 47537251 | 47537354 | 47537251 | ID=Msa0331570-mRNA-1:exon:14299;Parent=Msa0331570-mRNA-1 |
| chr2_4 | CDS | 47537251 | 47537354 | 47537251 | ID=Msa0331570-mRNA-1:cds;Parent=Msa0331570-mRNA-1 |
| chr2_4 | CDS | 47536716 | 47536836 | 47536716 | ID=Msa0331570-mRNA-1:cds;Parent=Msa0331570-mRNA-1 |
| chr2_4 | three_prime_UTR | 47536634 | 47536715 | 47536634 | ID=Msa0331570-mRNA-1:three_prime_utr;Parent=Msa0331570-mRNA-1 |
| Gene Sequence |
| Protein sequence |
