AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0993690


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0993690 MtrunA17_Chr8g0336401 93.617 141 9 0 1 141 144 284 2.09e-94 274
Msa0993690 MtrunA17_Chr8g0336411 86.525 141 16 1 1 141 144 281 4.19e-85 250
Msa0993690 MtrunA17_Chr8g0336441 73.529 136 36 0 6 141 143 278 4.49e-68 207
Msa0993690 MtrunA17_Chr3g0104871 74.074 135 35 0 7 141 136 270 8.08e-68 206
Msa0993690 MtrunA17_Chr8g0336391 61.151 139 52 1 3 141 145 281 3.94e-54 171
Msa0993690 MtrunA17_Chr8g0336381 51.938 129 61 1 14 141 134 262 7.63e-39 132
Msa0993690 MtrunA17_Chr5g0402871 46.099 141 72 3 2 141 122 259 4.20e-33 117
Msa0993690 MtrunA17_Chr5g0402851 45.070 142 74 3 1 141 124 262 1.66e-32 115
Msa0993690 MtrunA17_Chr5g0402841 46.923 130 66 3 13 141 134 261 1.16e-31 113
Msa0993690 MtrunA17_Chr8g0374141 37.069 116 70 2 26 141 190 302 6.22e-18 77.8
Msa0993690 MtrunA17_Chr5g0402901 38.261 115 66 3 28 141 151 261 8.55e-16 71.6
Msa0993690 MtrunA17_Chr5g0402881 35.897 117 70 3 26 141 166 278 3.72e-14 67.4
Msa0993690 MtrunA17_Chr5g0428471 33.333 132 81 3 11 141 155 280 9.49e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0993690 AT2G23570.1 44.262 122 63 3 21 141 61 178 3.89e-24 92.0
Msa0993690 AT2G23580.1 41.667 120 65 3 23 141 147 262 1.24e-21 87.4
Msa0993690 AT2G23610.1 36.691 139 85 2 3 141 127 262 3.43e-21 86.3
Msa0993690 AT3G50440.1 35.200 125 78 2 17 141 153 274 3.62e-20 84.0
Msa0993690 AT2G23620.1 37.705 122 73 2 20 141 144 262 5.87e-19 80.5
Msa0993690 AT2G23550.2 36.066 122 73 3 21 141 116 233 2.09e-17 75.9
Msa0993690 AT2G23550.1 36.066 122 73 3 21 141 145 262 3.24e-17 75.9
Msa0993690 AT2G23550.4 36.066 122 73 3 21 141 152 269 3.39e-17 75.9
Msa0993690 AT4G37150.1 43.269 104 56 2 38 141 155 255 4.68e-17 75.5
Msa0993690 AT2G23600.1 35.246 122 76 2 20 141 144 262 5.93e-17 75.1
Msa0993690 AT2G23600.2 35.246 122 76 2 20 141 159 277 7.69e-17 75.1
Msa0993690 AT2G23560.1 35.537 121 75 2 21 141 142 259 8.87e-16 72.0
Msa0993690 AT2G23590.1 31.579 114 75 2 28 141 161 271 3.61e-12 62.4
Msa0993690 AT2G23610.2 37.755 98 56 3 45 141 124 217 1.22e-11 60.1
Msa0993690 AT4G16690.1 31.159 138 81 6 5 137 128 256 5.30e-11 58.9

Find 34 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCCAACTGCCTTGTTGTT+TGG 0.223716 6_4:+66720931 Msa0993690:CDS
TTAGAGAGGATCTGCAATTA+TGG 0.262915 6_4:+66721616 Msa0993690:CDS
CCACAGTTATTCTTCATTGA+TGG 0.283394 6_4:+66720898 Msa0993690:CDS
ATCACTTTCACTTCAACATA+TGG 0.320896 6_4:-66721657 None:intergenic
TTGGAAATAATGAAGACTTT+TGG 0.329148 6_4:-66721579 None:intergenic
TCTAATGAAAATGTCTCCTT+TGG 0.376102 6_4:-66721598 None:intergenic
TACCCTTCCATTCCTCTGCT+TGG 0.389684 6_4:-66721556 None:intergenic
AAGGCAGTTGGAGGACTGTT+TGG 0.392141 6_4:-66720922 None:intergenic
TGTTGAAGTGAAAGTGATAA+AGG 0.400092 6_4:+66721662 Msa0993690:CDS
AAGATTAAATTCCAGTCAAC+AGG 0.402609 6_4:+66720870 Msa0993690:CDS
TTTGACACTTGGGTCATCAT+TGG 0.414427 6_4:+66721476 Msa0993690:CDS
GTTGTTTGGATCTAAACTCT+TGG 0.419417 6_4:+66720945 Msa0993690:CDS
CATGCGCAGGATTTGACACT+TGG 0.438141 6_4:+66721465 Msa0993690:intron
AGCAGTCTTTAGAATGTAAG+AGG 0.485742 6_4:-66721727 None:intergenic
GTATCAATTATCGCCTAATG+AGG 0.511231 6_4:+66720978 Msa0993690:CDS
AATTGGTGTTGAAAGAAGCA+AGG 0.519211 6_4:+66721529 Msa0993690:CDS
AAAGTCTTCATTATTTCCAA+AGG 0.521901 6_4:+66721582 Msa0993690:CDS
TCGTCATTAAAGATAGGATG+TGG 0.526108 6_4:-66721504 None:intergenic
TTATGGATAATTGAGAGAAC+GGG 0.528041 6_4:+66721633 Msa0993690:CDS
AGGGTTACCAAGCAGAGGAA+TGG 0.528556 6_4:+66721549 Msa0993690:CDS
GATCCAAACAACAAGGCAGT+TGG 0.532373 6_4:-66720934 None:intergenic
ATTATGGATAATTGAGAGAA+CGG 0.544632 6_4:+66721632 Msa0993690:CDS
CTGTGGTGGATCCTGTTGAC+TGG 0.559384 6_4:-66720881 None:intergenic
ATTGGTGTTGAAAGAAGCAA+GGG 0.568194 6_4:+66721530 Msa0993690:CDS
CCAAACAACAAGGCAGTTGG+AGG 0.578597 6_4:-66720931 None:intergenic
TTACCAAGCAGAGGAATGGA+AGG 0.593361 6_4:+66721553 Msa0993690:CDS
CACTTGGGTCATCATTGGTG+AGG 0.603881 6_4:+66721481 Msa0993690:CDS
AAGCAAGGGTTACCAAGCAG+AGG 0.608021 6_4:+66721544 Msa0993690:CDS
TCAATGAAGAATAACTGTGG+TGG 0.619467 6_4:-66720895 None:intergenic
ATGCGCAGGATTTGACACTT+GGG 0.634134 6_4:+66721466 Msa0993690:intron
GATAAAGGATTCAGATCACA+TGG 0.658047 6_4:+66721677 Msa0993690:CDS
TACCAAGCAGAGGAATGGAA+GGG 0.694878 6_4:+66721554 Msa0993690:CDS
GAGTTTAGATCCAAACAACA+AGG 0.704963 6_4:-66720941 None:intergenic
CCATCAATGAAGAATAACTG+TGG 0.765316 6_4:-66720898 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTGAAAATTTGAAATAA+AGG + chr6_4:66721016-66721035 Msa0993690:intron 10.0%
!!! TAATTGAAAATTTGAAATAA+AGG + chr6_4:66721016-66721035 Msa0993690:intron 10.0%
!! CTGTTAAAAATAATTTATCT+AGG + chr6_4:66721215-66721234 Msa0993690:intron 15.0%
!! TAATATAATATGGTATGTTA+AGG - chr6_4:66721396-66721415 None:intergenic 15.0%
!! CTGTTAAAAATAATTTATCT+AGG + chr6_4:66721215-66721234 Msa0993690:intron 15.0%
!! TAATATAATATGGTATGTTA+AGG - chr6_4:66721396-66721415 None:intergenic 15.0%
!! AACAAAAAAATTACCTCATT+AGG - chr6_4:66720994-66721013 None:intergenic 20.0%
!!! AATTTTTCGTCATTAAAGAT+AGG - chr6_4:66721513-66721532 None:intergenic 20.0%
!! TATCTTTAATGACGAAAAAT+TGG + chr6_4:66721512-66721531 Msa0993690:CDS 20.0%
!! AACAAAAAAATTACCTCATT+AGG - chr6_4:66720994-66721013 None:intergenic 20.0%
!!! AATTTTTCGTCATTAAAGAT+AGG - chr6_4:66721513-66721532 None:intergenic 20.0%
!! TATCTTTAATGACGAAAAAT+TGG + chr6_4:66721512-66721531 Msa0993690:CDS 20.0%
! CGATTAAACTTCAAATCATT+AGG + chr6_4:66721296-66721315 Msa0993690:intron 25.0%
! TTAAACTTCAAATCATTAGG+AGG + chr6_4:66721299-66721318 Msa0993690:intron 25.0%
!! TTGGAAATAATGAAGACTTT+TGG - chr6_4:66721582-66721601 None:intergenic 25.0%
! AAAGTCTTCATTATTTCCAA+AGG + chr6_4:66721582-66721601 Msa0993690:CDS 25.0%
! ATTATGGATAATTGAGAGAA+CGG + chr6_4:66721632-66721651 Msa0993690:CDS 25.0%
! CGATTAAACTTCAAATCATT+AGG + chr6_4:66721296-66721315 Msa0993690:intron 25.0%
! TTAAACTTCAAATCATTAGG+AGG + chr6_4:66721299-66721318 Msa0993690:intron 25.0%
!! TTGGAAATAATGAAGACTTT+TGG - chr6_4:66721582-66721601 None:intergenic 25.0%
! AAAGTCTTCATTATTTCCAA+AGG + chr6_4:66721582-66721601 Msa0993690:CDS 25.0%
! ATTATGGATAATTGAGAGAA+CGG + chr6_4:66721632-66721651 Msa0993690:CDS 25.0%
AAGATTAAATTCCAGTCAAC+AGG + chr6_4:66720870-66720889 Msa0993690:CDS 30.0%
TCTAATGAAAATGTCTCCTT+TGG - chr6_4:66721601-66721620 None:intergenic 30.0%
TTATGGATAATTGAGAGAAC+GGG + chr6_4:66721633-66721652 Msa0993690:CDS 30.0%
ATCACTTTCACTTCAACATA+TGG - chr6_4:66721660-66721679 None:intergenic 30.0%
TGTTGAAGTGAAAGTGATAA+AGG + chr6_4:66721662-66721681 Msa0993690:CDS 30.0%
! ATGTAAGAGGTAAGCTTTTT+AGG - chr6_4:66721717-66721736 None:intergenic 30.0%
AAGATTAAATTCCAGTCAAC+AGG + chr6_4:66720870-66720889 Msa0993690:CDS 30.0%
TCTAATGAAAATGTCTCCTT+TGG - chr6_4:66721601-66721620 None:intergenic 30.0%
TTATGGATAATTGAGAGAAC+GGG + chr6_4:66721633-66721652 Msa0993690:CDS 30.0%
ATCACTTTCACTTCAACATA+TGG - chr6_4:66721660-66721679 None:intergenic 30.0%
TGTTGAAGTGAAAGTGATAA+AGG + chr6_4:66721662-66721681 Msa0993690:CDS 30.0%
! ATGTAAGAGGTAAGCTTTTT+AGG - chr6_4:66721717-66721736 None:intergenic 30.0%
TCAATGAAGAATAACTGTGG+TGG - chr6_4:66720898-66720917 None:intergenic 35.0%
CCATCAATGAAGAATAACTG+TGG - chr6_4:66720901-66720920 None:intergenic 35.0%
CCACAGTTATTCTTCATTGA+TGG + chr6_4:66720898-66720917 Msa0993690:CDS 35.0%
GAGTTTAGATCCAAACAACA+AGG - chr6_4:66720944-66720963 None:intergenic 35.0%
GTTGTTTGGATCTAAACTCT+TGG + chr6_4:66720945-66720964 Msa0993690:CDS 35.0%
GTATCAATTATCGCCTAATG+AGG + chr6_4:66720978-66720997 Msa0993690:CDS 35.0%
ACATTGAAGCCAATCATTGA+TGG + chr6_4:66721439-66721458 Msa0993690:intron 35.0%
TCGTCATTAAAGATAGGATG+TGG - chr6_4:66721507-66721526 None:intergenic 35.0%
!! AATTGGTGTTGAAAGAAGCA+AGG + chr6_4:66721529-66721548 Msa0993690:CDS 35.0%
!! ATTGGTGTTGAAAGAAGCAA+GGG + chr6_4:66721530-66721549 Msa0993690:CDS 35.0%
! AGGAGACATTTTCATTAGAG+AGG + chr6_4:66721602-66721621 Msa0993690:CDS 35.0%
TTAGAGAGGATCTGCAATTA+TGG + chr6_4:66721616-66721635 Msa0993690:CDS 35.0%
GATAAAGGATTCAGATCACA+TGG + chr6_4:66721677-66721696 Msa0993690:CDS 35.0%
!!! AGAGGTAAGCTTTTTAGGTT+TGG - chr6_4:66721712-66721731 None:intergenic 35.0%
!! AGCAGTCTTTAGAATGTAAG+AGG - chr6_4:66721730-66721749 None:intergenic 35.0%
TCAATGAAGAATAACTGTGG+TGG - chr6_4:66720898-66720917 None:intergenic 35.0%
CCATCAATGAAGAATAACTG+TGG - chr6_4:66720901-66720920 None:intergenic 35.0%
CCACAGTTATTCTTCATTGA+TGG + chr6_4:66720898-66720917 Msa0993690:CDS 35.0%
GAGTTTAGATCCAAACAACA+AGG - chr6_4:66720944-66720963 None:intergenic 35.0%
GTTGTTTGGATCTAAACTCT+TGG + chr6_4:66720945-66720964 Msa0993690:CDS 35.0%
GTATCAATTATCGCCTAATG+AGG + chr6_4:66720978-66720997 Msa0993690:CDS 35.0%
ACATTGAAGCCAATCATTGA+TGG + chr6_4:66721439-66721458 Msa0993690:intron 35.0%
TCGTCATTAAAGATAGGATG+TGG - chr6_4:66721507-66721526 None:intergenic 35.0%
!! AATTGGTGTTGAAAGAAGCA+AGG + chr6_4:66721529-66721548 Msa0993690:CDS 35.0%
!! ATTGGTGTTGAAAGAAGCAA+GGG + chr6_4:66721530-66721549 Msa0993690:CDS 35.0%
! AGGAGACATTTTCATTAGAG+AGG + chr6_4:66721602-66721621 Msa0993690:CDS 35.0%
TTAGAGAGGATCTGCAATTA+TGG + chr6_4:66721616-66721635 Msa0993690:CDS 35.0%
GATAAAGGATTCAGATCACA+TGG + chr6_4:66721677-66721696 Msa0993690:CDS 35.0%
!!! AGAGGTAAGCTTTTTAGGTT+TGG - chr6_4:66721712-66721731 None:intergenic 35.0%
!! AGCAGTCTTTAGAATGTAAG+AGG - chr6_4:66721730-66721749 None:intergenic 35.0%
CGCATGTAACCATCAATGAT+TGG - chr6_4:66721451-66721470 None:intergenic 40.0%
TTTGACACTTGGGTCATCAT+TGG + chr6_4:66721476-66721495 Msa0993690:CDS 40.0%
CGCATGTAACCATCAATGAT+TGG - chr6_4:66721451-66721470 None:intergenic 40.0%
TTTGACACTTGGGTCATCAT+TGG + chr6_4:66721476-66721495 Msa0993690:CDS 40.0%
GATCCAAACAACAAGGCAGT+TGG - chr6_4:66720937-66720956 None:intergenic 45.0%
! TCATTGATGGTTACATGCGC+AGG + chr6_4:66721452-66721471 Msa0993690:intron 45.0%
ATGCGCAGGATTTGACACTT+GGG + chr6_4:66721466-66721485 Msa0993690:intron 45.0%
TTACCAAGCAGAGGAATGGA+AGG + chr6_4:66721553-66721572 Msa0993690:CDS 45.0%
TACCAAGCAGAGGAATGGAA+GGG + chr6_4:66721554-66721573 Msa0993690:CDS 45.0%
GATCCAAACAACAAGGCAGT+TGG - chr6_4:66720937-66720956 None:intergenic 45.0%
! TCATTGATGGTTACATGCGC+AGG + chr6_4:66721452-66721471 Msa0993690:intron 45.0%
ATGCGCAGGATTTGACACTT+GGG + chr6_4:66721466-66721485 Msa0993690:intron 45.0%
TTACCAAGCAGAGGAATGGA+AGG + chr6_4:66721553-66721572 Msa0993690:CDS 45.0%
TACCAAGCAGAGGAATGGAA+GGG + chr6_4:66721554-66721573 Msa0993690:CDS 45.0%
!! ATGTATAATATAATATAATA+TGG - chr6_4:66721406-66721425 None:intergenic 5.0%
!! ATGTATAATATAATATAATA+TGG - chr6_4:66721406-66721425 None:intergenic 5.0%
AAGGCAGTTGGAGGACTGTT+TGG - chr6_4:66720925-66720944 None:intergenic 50.0%
CCAAACAACAAGGCAGTTGG+AGG - chr6_4:66720934-66720953 None:intergenic 50.0%
!! CCTCCAACTGCCTTGTTGTT+TGG + chr6_4:66720931-66720950 Msa0993690:CDS 50.0%
CATGCGCAGGATTTGACACT+TGG + chr6_4:66721465-66721484 Msa0993690:intron 50.0%
CACTTGGGTCATCATTGGTG+AGG + chr6_4:66721481-66721500 Msa0993690:CDS 50.0%
AAGCAAGGGTTACCAAGCAG+AGG + chr6_4:66721544-66721563 Msa0993690:CDS 50.0%
AGGGTTACCAAGCAGAGGAA+TGG + chr6_4:66721549-66721568 Msa0993690:CDS 50.0%
TACCCTTCCATTCCTCTGCT+TGG - chr6_4:66721559-66721578 None:intergenic 50.0%
AAGGCAGTTGGAGGACTGTT+TGG - chr6_4:66720925-66720944 None:intergenic 50.0%
CCAAACAACAAGGCAGTTGG+AGG - chr6_4:66720934-66720953 None:intergenic 50.0%
!! CCTCCAACTGCCTTGTTGTT+TGG + chr6_4:66720931-66720950 Msa0993690:CDS 50.0%
CATGCGCAGGATTTGACACT+TGG + chr6_4:66721465-66721484 Msa0993690:intron 50.0%
CACTTGGGTCATCATTGGTG+AGG + chr6_4:66721481-66721500 Msa0993690:CDS 50.0%
AAGCAAGGGTTACCAAGCAG+AGG + chr6_4:66721544-66721563 Msa0993690:CDS 50.0%
AGGGTTACCAAGCAGAGGAA+TGG + chr6_4:66721549-66721568 Msa0993690:CDS 50.0%
TACCCTTCCATTCCTCTGCT+TGG - chr6_4:66721559-66721578 None:intergenic 50.0%
!! CTGTGGTGGATCCTGTTGAC+TGG - chr6_4:66720884-66720903 None:intergenic 55.0%
!! CTGTGGTGGATCCTGTTGAC+TGG - chr6_4:66720884-66720903 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 66720862 66721761 66720862 ID=Msa0993690;Name=Msa0993690
chr6_4 mRNA 66720862 66721761 66720862 ID=Msa0993690-mRNA-1;Parent=Msa0993690;Name=Msa0993690-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|1|1|2|0|141
chr6_4 exon 66720862 66720999 66720862 ID=Msa0993690-mRNA-1:exon:12448;Parent=Msa0993690-mRNA-1
chr6_4 exon 66721474 66721761 66721474 ID=Msa0993690-mRNA-1:exon:12449;Parent=Msa0993690-mRNA-1
chr6_4 CDS 66720862 66720999 66720862 ID=Msa0993690-mRNA-1:cds;Parent=Msa0993690-mRNA-1
chr6_4 CDS 66721474 66721761 66721474 ID=Msa0993690-mRNA-1:cds;Parent=Msa0993690-mRNA-1
Gene Sequence

>Msa0993690

AATCAGAGAAGATTAAATTCCAGTCAACAGGATCCACCACAGTTATTCTTCATTGATGGACCAAACAGTCCTCCAACTGCCTTGTTGTTTGGATCTAAACTCTTGGCATCTAATTTGTATCAATTATCGCCTAATGAGGATTTGACACTTGGGTCATCATTGGTGAGGCCACATCCTATCTTTAATGACGAAAAATTGGTGTTGAAAGAAGCAAGGGTTACCAAGCAGAGGAATGGAAGGGTACCAAAAGTCTTCATTATTTCCAAAGGAGACATTTTCATTAGAGAGGATCTGCAATTATGGATAATTGAGAGAACGGGTCCATATGTTGAAGTGAAAGTGATAAAGGATTCAGATCACATGGTCATGTTCTCCAAACCTAAAAAGCTTACCTCTTACATTCTAAAGACTGCTCACAAATATTAG

Protein sequence

>Msa0993690

NQRRLNSSQQDPPQLFFIDGPNSPPTALLFGSKLLASNLYQLSPNEDLTLGSSLVRPHPIFNDEKLVLKEARVTKQRNGRVPKVFIISKGDIFIREDLQLWIIERTGPYVEVKVIKDSDHMVMFSKPKKLTSYILKTAHKY*