AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1309400


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1309400 MtrunA17_Chr8g0336401 93.617 141 9 0 1 141 144 284 1.98e-94 274
Msa1309400 MtrunA17_Chr8g0336411 87.234 141 15 1 1 141 144 281 3.99e-86 253
Msa1309400 MtrunA17_Chr3g0104871 74.815 135 34 0 7 141 136 270 1.34e-68 208
Msa1309400 MtrunA17_Chr8g0336441 73.529 136 36 0 6 141 143 278 5.71e-68 206
Msa1309400 MtrunA17_Chr8g0336391 61.871 139 51 1 3 141 145 281 4.26e-55 174
Msa1309400 MtrunA17_Chr8g0336381 51.938 129 61 1 14 141 134 262 2.19e-38 130
Msa1309400 MtrunA17_Chr5g0402871 46.099 141 72 3 2 141 122 259 5.49e-33 116
Msa1309400 MtrunA17_Chr5g0402851 45.070 142 74 3 1 141 124 262 1.73e-32 115
Msa1309400 MtrunA17_Chr5g0402841 46.923 130 66 3 13 141 134 261 1.30e-31 113
Msa1309400 MtrunA17_Chr8g0374141 37.069 116 70 2 26 141 190 302 7.71e-18 77.8
Msa1309400 MtrunA17_Chr5g0402901 38.261 115 66 3 28 141 151 261 7.71e-16 72.0
Msa1309400 MtrunA17_Chr5g0402881 36.000 125 72 4 18 141 161 278 1.57e-14 68.6
Msa1309400 MtrunA17_Chr5g0428471 33.333 132 81 3 11 141 155 280 7.84e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1309400 AT2G23570.1 43.200 125 66 3 18 141 58 178 2.00e-24 92.8
Msa1309400 AT2G23580.1 42.982 114 60 3 29 141 153 262 1.31e-21 87.4
Msa1309400 AT2G23610.1 36.691 139 85 2 3 141 127 262 4.02e-21 86.3
Msa1309400 AT3G50440.1 35.200 125 78 2 17 141 153 274 3.70e-20 84.0
Msa1309400 AT2G23620.1 37.705 122 73 2 20 141 144 262 6.25e-19 80.5
Msa1309400 AT2G23550.2 35.200 125 76 3 18 141 113 233 1.72e-17 76.3
Msa1309400 AT2G23550.1 35.200 125 76 3 18 141 142 262 2.69e-17 76.3
Msa1309400 AT2G23550.4 35.200 125 76 3 18 141 149 269 3.05e-17 76.3
Msa1309400 AT4G37150.1 43.269 104 56 2 38 141 155 255 4.05e-17 75.5
Msa1309400 AT2G23600.1 35.246 122 76 2 20 141 144 262 6.51e-17 75.1
Msa1309400 AT2G23600.2 35.246 122 76 2 20 141 159 277 8.53e-17 75.1
Msa1309400 AT2G23560.1 35.484 124 77 2 18 141 139 259 4.02e-16 72.8
Msa1309400 AT2G23590.1 31.579 114 75 2 28 141 161 271 3.76e-12 62.4
Msa1309400 AT2G23610.2 37.755 98 56 3 45 141 124 217 1.30e-11 60.1
Msa1309400 AT4G16690.1 31.159 138 81 6 5 137 128 256 5.68e-11 58.9

Find 34 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCCAACTGCCTTGTTGTT+TGG 0.223716 8_4:-5874456 Msa1309400:CDS
CCACAGTTATTCTTCTTTGA+TGG 0.234931 8_4:-5874489 Msa1309400:CDS
TTAGAGAGGATCTGCAATTA+TGG 0.262915 8_4:-5873771 Msa1309400:CDS
ATCACTTTCACTTCAACATA+TGG 0.320896 8_4:+5873730 None:intergenic
TTGGAAATAATGAAGACTTT+TGG 0.329148 8_4:+5873808 None:intergenic
TCTAATGAAAATGTCTCCTT+TGG 0.376102 8_4:+5873789 None:intergenic
TACCCTTCCATTCCTCTGCT+TGG 0.389684 8_4:+5873831 None:intergenic
AAGGCAGTTGGAGGACTGTT+TGG 0.392141 8_4:+5874465 None:intergenic
TGTTGAAGTGAAAGTGATAA+AGG 0.400092 8_4:-5873725 Msa1309400:CDS
AAGATTAAATTCCAGTCAAC+AGG 0.402609 8_4:-5874517 Msa1309400:CDS
TTTGACACTTGGGTCATCAT+TGG 0.414427 8_4:-5873911 Msa1309400:CDS
GTTGTTTGGATCTAAACTCT+TGG 0.419417 8_4:-5874442 Msa1309400:CDS
CATGCGCAGGATTTGACACT+TGG 0.438141 8_4:-5873922 Msa1309400:intron
AGCAGTCTTTAGAATGTAAG+AGG 0.485742 8_4:+5873660 None:intergenic
GTATCAATTATCGCCTAATG+AGG 0.511231 8_4:-5874409 Msa1309400:intron
AATTGGTGTTGAAAGAAGCA+AGG 0.519211 8_4:-5873858 Msa1309400:CDS
AAAGTCTTCATTATTTCCAA+AGG 0.521901 8_4:-5873805 Msa1309400:CDS
TCGTCATTAAAGATAGGATG+TGG 0.526108 8_4:+5873883 None:intergenic
TTATGGATAATTGAGAGAAC+GGG 0.528041 8_4:-5873754 Msa1309400:CDS
AGGGTTACCAAGCAGAGGAA+TGG 0.528556 8_4:-5873838 Msa1309400:CDS
GATCCAAACAACAAGGCAGT+TGG 0.532373 8_4:+5874453 None:intergenic
ATTATGGATAATTGAGAGAA+CGG 0.544632 8_4:-5873755 Msa1309400:CDS
CTGTGGTGGATCCTGTTGAC+TGG 0.559384 8_4:+5874506 None:intergenic
ATTGGTGTTGAAAGAAGCAA+GGG 0.568194 8_4:-5873857 Msa1309400:CDS
CCAAACAACAAGGCAGTTGG+AGG 0.578597 8_4:+5874456 None:intergenic
TTACCAAGCAGAGGAATGGA+AGG 0.593361 8_4:-5873834 Msa1309400:CDS
TCAAAGAAGAATAACTGTGG+TGG 0.601293 8_4:+5874492 None:intergenic
CACTTGGGTCATCATTGGTG+AGG 0.603881 8_4:-5873906 Msa1309400:CDS
AAGCAAGGGTTACCAAGCAG+AGG 0.608021 8_4:-5873843 Msa1309400:CDS
ATGCGCAGGATTTGACACTT+GGG 0.634134 8_4:-5873921 Msa1309400:intron
GATAAAGGATTCAGATCACA+TGG 0.658047 8_4:-5873710 Msa1309400:CDS
TACCAAGCAGAGGAATGGAA+GGG 0.694878 8_4:-5873833 Msa1309400:CDS
GAGTTTAGATCCAAACAACA+AGG 0.704963 8_4:+5874446 None:intergenic
CCATCAAAGAAGAATAACTG+TGG 0.722018 8_4:+5874489 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTGAAAATTTGAAATAA+AGG - chr8_4:5873802-5873821 Msa1309400:CDS 10.0%
!!! TAATTGAAAATTTGAAATAA+AGG - chr8_4:5873802-5873821 Msa1309400:CDS 10.0%
!! CTGTTAAAAATAATTTATCT+AGG - chr8_4:5874001-5874020 Msa1309400:intron 15.0%
!! TAATATAATATGGTATGTTA+AGG + chr8_4:5874182-5874201 None:intergenic 15.0%
!! CTGTTAAAAATAATTTATCT+AGG - chr8_4:5874001-5874020 Msa1309400:intron 15.0%
!! TAATATAATATGGTATGTTA+AGG + chr8_4:5874182-5874201 None:intergenic 15.0%
!! AACAAAAAAATTACCTCATT+AGG + chr8_4:5873780-5873799 None:intergenic 20.0%
!!! AATTTTTCGTCATTAAAGAT+AGG + chr8_4:5874299-5874318 None:intergenic 20.0%
!! TATCTTTAATGACGAAAAAT+TGG - chr8_4:5874298-5874317 Msa1309400:intron 20.0%
!! AACAAAAAAATTACCTCATT+AGG + chr8_4:5873780-5873799 None:intergenic 20.0%
!!! AATTTTTCGTCATTAAAGAT+AGG + chr8_4:5874299-5874318 None:intergenic 20.0%
!! TATCTTTAATGACGAAAAAT+TGG - chr8_4:5874298-5874317 Msa1309400:intron 20.0%
! CGATTAAACTTCAAATCATT+AGG - chr8_4:5874082-5874101 Msa1309400:intron 25.0%
! TTAAACTTCAAATCATTAGG+AGG - chr8_4:5874085-5874104 Msa1309400:intron 25.0%
!! TTGGAAATAATGAAGACTTT+TGG + chr8_4:5874368-5874387 None:intergenic 25.0%
! AAAGTCTTCATTATTTCCAA+AGG - chr8_4:5874368-5874387 Msa1309400:intron 25.0%
! ATTATGGATAATTGAGAGAA+CGG - chr8_4:5874418-5874437 Msa1309400:CDS 25.0%
! CGATTAAACTTCAAATCATT+AGG - chr8_4:5874082-5874101 Msa1309400:intron 25.0%
! TTAAACTTCAAATCATTAGG+AGG - chr8_4:5874085-5874104 Msa1309400:intron 25.0%
!! TTGGAAATAATGAAGACTTT+TGG + chr8_4:5874368-5874387 None:intergenic 25.0%
! AAAGTCTTCATTATTTCCAA+AGG - chr8_4:5874368-5874387 Msa1309400:intron 25.0%
! ATTATGGATAATTGAGAGAA+CGG - chr8_4:5874418-5874437 Msa1309400:CDS 25.0%
AAGATTAAATTCCAGTCAAC+AGG - chr8_4:5873656-5873675 Msa1309400:CDS 30.0%
TCTAATGAAAATGTCTCCTT+TGG + chr8_4:5874387-5874406 None:intergenic 30.0%
TTATGGATAATTGAGAGAAC+GGG - chr8_4:5874419-5874438 Msa1309400:CDS 30.0%
ATCACTTTCACTTCAACATA+TGG + chr8_4:5874446-5874465 None:intergenic 30.0%
TGTTGAAGTGAAAGTGATAA+AGG - chr8_4:5874448-5874467 Msa1309400:CDS 30.0%
! ATGTAAGAGGTAAGCTTTTT+AGG + chr8_4:5874503-5874522 None:intergenic 30.0%
AAGATTAAATTCCAGTCAAC+AGG - chr8_4:5873656-5873675 Msa1309400:CDS 30.0%
TCTAATGAAAATGTCTCCTT+TGG + chr8_4:5874387-5874406 None:intergenic 30.0%
TTATGGATAATTGAGAGAAC+GGG - chr8_4:5874419-5874438 Msa1309400:CDS 30.0%
ATCACTTTCACTTCAACATA+TGG + chr8_4:5874446-5874465 None:intergenic 30.0%
TGTTGAAGTGAAAGTGATAA+AGG - chr8_4:5874448-5874467 Msa1309400:CDS 30.0%
! ATGTAAGAGGTAAGCTTTTT+AGG + chr8_4:5874503-5874522 None:intergenic 30.0%
TCAAAGAAGAATAACTGTGG+TGG + chr8_4:5873684-5873703 None:intergenic 35.0%
CCATCAAAGAAGAATAACTG+TGG + chr8_4:5873687-5873706 None:intergenic 35.0%
CCACAGTTATTCTTCTTTGA+TGG - chr8_4:5873684-5873703 Msa1309400:CDS 35.0%
GAGTTTAGATCCAAACAACA+AGG + chr8_4:5873730-5873749 None:intergenic 35.0%
GTTGTTTGGATCTAAACTCT+TGG - chr8_4:5873731-5873750 Msa1309400:CDS 35.0%
GTATCAATTATCGCCTAATG+AGG - chr8_4:5873764-5873783 Msa1309400:CDS 35.0%
ACATTGAAGCCAATCATTGA+TGG - chr8_4:5874225-5874244 Msa1309400:intron 35.0%
TCGTCATTAAAGATAGGATG+TGG + chr8_4:5874293-5874312 None:intergenic 35.0%
!! AATTGGTGTTGAAAGAAGCA+AGG - chr8_4:5874315-5874334 Msa1309400:intron 35.0%
!! ATTGGTGTTGAAAGAAGCAA+GGG - chr8_4:5874316-5874335 Msa1309400:intron 35.0%
! AGGAGACATTTTCATTAGAG+AGG - chr8_4:5874388-5874407 Msa1309400:intron 35.0%
TTAGAGAGGATCTGCAATTA+TGG - chr8_4:5874402-5874421 Msa1309400:intron 35.0%
GATAAAGGATTCAGATCACA+TGG - chr8_4:5874463-5874482 Msa1309400:CDS 35.0%
!!! AGAGGTAAGCTTTTTAGGTT+TGG + chr8_4:5874498-5874517 None:intergenic 35.0%
!! AGCAGTCTTTAGAATGTAAG+AGG + chr8_4:5874516-5874535 None:intergenic 35.0%
TCAAAGAAGAATAACTGTGG+TGG + chr8_4:5873684-5873703 None:intergenic 35.0%
CCATCAAAGAAGAATAACTG+TGG + chr8_4:5873687-5873706 None:intergenic 35.0%
CCACAGTTATTCTTCTTTGA+TGG - chr8_4:5873684-5873703 Msa1309400:CDS 35.0%
GAGTTTAGATCCAAACAACA+AGG + chr8_4:5873730-5873749 None:intergenic 35.0%
GTTGTTTGGATCTAAACTCT+TGG - chr8_4:5873731-5873750 Msa1309400:CDS 35.0%
GTATCAATTATCGCCTAATG+AGG - chr8_4:5873764-5873783 Msa1309400:CDS 35.0%
ACATTGAAGCCAATCATTGA+TGG - chr8_4:5874225-5874244 Msa1309400:intron 35.0%
TCGTCATTAAAGATAGGATG+TGG + chr8_4:5874293-5874312 None:intergenic 35.0%
!! AATTGGTGTTGAAAGAAGCA+AGG - chr8_4:5874315-5874334 Msa1309400:intron 35.0%
!! ATTGGTGTTGAAAGAAGCAA+GGG - chr8_4:5874316-5874335 Msa1309400:intron 35.0%
! AGGAGACATTTTCATTAGAG+AGG - chr8_4:5874388-5874407 Msa1309400:intron 35.0%
TTAGAGAGGATCTGCAATTA+TGG - chr8_4:5874402-5874421 Msa1309400:intron 35.0%
GATAAAGGATTCAGATCACA+TGG - chr8_4:5874463-5874482 Msa1309400:CDS 35.0%
!!! AGAGGTAAGCTTTTTAGGTT+TGG + chr8_4:5874498-5874517 None:intergenic 35.0%
!! AGCAGTCTTTAGAATGTAAG+AGG + chr8_4:5874516-5874535 None:intergenic 35.0%
CGCATGTAACCATCAATGAT+TGG + chr8_4:5874237-5874256 None:intergenic 40.0%
TTTGACACTTGGGTCATCAT+TGG - chr8_4:5874262-5874281 Msa1309400:intron 40.0%
CGCATGTAACCATCAATGAT+TGG + chr8_4:5874237-5874256 None:intergenic 40.0%
TTTGACACTTGGGTCATCAT+TGG - chr8_4:5874262-5874281 Msa1309400:intron 40.0%
GATCCAAACAACAAGGCAGT+TGG + chr8_4:5873723-5873742 None:intergenic 45.0%
! TCATTGATGGTTACATGCGC+AGG - chr8_4:5874238-5874257 Msa1309400:intron 45.0%
ATGCGCAGGATTTGACACTT+GGG - chr8_4:5874252-5874271 Msa1309400:intron 45.0%
TTACCAAGCAGAGGAATGGA+AGG - chr8_4:5874339-5874358 Msa1309400:intron 45.0%
TACCAAGCAGAGGAATGGAA+GGG - chr8_4:5874340-5874359 Msa1309400:intron 45.0%
GATCCAAACAACAAGGCAGT+TGG + chr8_4:5873723-5873742 None:intergenic 45.0%
! TCATTGATGGTTACATGCGC+AGG - chr8_4:5874238-5874257 Msa1309400:intron 45.0%
ATGCGCAGGATTTGACACTT+GGG - chr8_4:5874252-5874271 Msa1309400:intron 45.0%
TTACCAAGCAGAGGAATGGA+AGG - chr8_4:5874339-5874358 Msa1309400:intron 45.0%
TACCAAGCAGAGGAATGGAA+GGG - chr8_4:5874340-5874359 Msa1309400:intron 45.0%
!! ATGTATAATATAATATAATA+TGG + chr8_4:5874192-5874211 None:intergenic 5.0%
!! ATGTATAATATAATATAATA+TGG + chr8_4:5874192-5874211 None:intergenic 5.0%
AAGGCAGTTGGAGGACTGTT+TGG + chr8_4:5873711-5873730 None:intergenic 50.0%
CCAAACAACAAGGCAGTTGG+AGG + chr8_4:5873720-5873739 None:intergenic 50.0%
!! CCTCCAACTGCCTTGTTGTT+TGG - chr8_4:5873717-5873736 Msa1309400:CDS 50.0%
CATGCGCAGGATTTGACACT+TGG - chr8_4:5874251-5874270 Msa1309400:intron 50.0%
CACTTGGGTCATCATTGGTG+AGG - chr8_4:5874267-5874286 Msa1309400:intron 50.0%
AAGCAAGGGTTACCAAGCAG+AGG - chr8_4:5874330-5874349 Msa1309400:intron 50.0%
AGGGTTACCAAGCAGAGGAA+TGG - chr8_4:5874335-5874354 Msa1309400:intron 50.0%
TACCCTTCCATTCCTCTGCT+TGG + chr8_4:5874345-5874364 None:intergenic 50.0%
AAGGCAGTTGGAGGACTGTT+TGG + chr8_4:5873711-5873730 None:intergenic 50.0%
CCAAACAACAAGGCAGTTGG+AGG + chr8_4:5873720-5873739 None:intergenic 50.0%
!! CCTCCAACTGCCTTGTTGTT+TGG - chr8_4:5873717-5873736 Msa1309400:CDS 50.0%
CATGCGCAGGATTTGACACT+TGG - chr8_4:5874251-5874270 Msa1309400:intron 50.0%
CACTTGGGTCATCATTGGTG+AGG - chr8_4:5874267-5874286 Msa1309400:intron 50.0%
AAGCAAGGGTTACCAAGCAG+AGG - chr8_4:5874330-5874349 Msa1309400:intron 50.0%
AGGGTTACCAAGCAGAGGAA+TGG - chr8_4:5874335-5874354 Msa1309400:intron 50.0%
TACCCTTCCATTCCTCTGCT+TGG + chr8_4:5874345-5874364 None:intergenic 50.0%
!! CTGTGGTGGATCCTGTTGAC+TGG + chr8_4:5873670-5873689 None:intergenic 55.0%
!! CTGTGGTGGATCCTGTTGAC+TGG + chr8_4:5873670-5873689 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 5873648 5874547 5873648 ID=Msa1309400;Name=Msa1309400
chr8_4 mRNA 5873648 5874547 5873648 ID=Msa1309400-mRNA-1;Parent=Msa1309400;Name=Msa1309400-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|1|1|2|0|141
chr8_4 exon 5874410 5874547 5874410 ID=Msa1309400-mRNA-1:exon:1969;Parent=Msa1309400-mRNA-1
chr8_4 exon 5873648 5873935 5873648 ID=Msa1309400-mRNA-1:exon:1968;Parent=Msa1309400-mRNA-1
chr8_4 CDS 5874410 5874547 5874410 ID=Msa1309400-mRNA-1:cds;Parent=Msa1309400-mRNA-1
chr8_4 CDS 5873648 5873935 5873648 ID=Msa1309400-mRNA-1:cds;Parent=Msa1309400-mRNA-1
Gene Sequence

>Msa1309400

AATCAGAGAAGATTAAATTCCAGTCAACAGGATCCACCACAGTTATTCTTCTTTGATGGACCAAACAGTCCTCCAACTGCCTTGTTGTTTGGATCTAAACTCTTGGCATCTAATTTGTATCAATTATCGCCTAATGAGGATTTGACACTTGGGTCATCATTGGTGAGGCCACATCCTATCTTTAATGACGAAAAATTGGTGTTGAAAGAAGCAAGGGTTACCAAGCAGAGGAATGGAAGGGTACCAAAAGTCTTCATTATTTCCAAAGGAGACATTTTCATTAGAGAGGATCTGCAATTATGGATAATTGAGAGAACGGGTCCATATGTTGAAGTGAAAGTGATAAAGGATTCAGATCACATGGTCATGTTCTCCAAACCTAAAAAGCTTACCTCTTACATTCTAAAGACTGCTCACAAATATTAG

Protein sequence

>Msa1309400

NQRRLNSSQQDPPQLFFFDGPNSPPTALLFGSKLLASNLYQLSPNEDLTLGSSLVRPHPIFNDEKLVLKEARVTKQRNGRVPKVFIISKGDIFIREDLQLWIIERTGPYVEVKVIKDSDHMVMFSKPKKLTSYILKTAHKY*