AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0994520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0994520 MtrunA17_Chr6g0478641 94.776 134 7 0 1 134 1 134 1.51e-81 236
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0994520 AT5G52220.2 64.912 114 38 2 1 113 1 113 2.56e-35 118
Msa0994520 AT5G52220.1 64.912 114 38 2 1 113 1 113 9.86e-35 117

Find 74 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAAACTTCTCAAAATTTC+TGG 0.111329 6_4:-69519802 None:intergenic
TTATTTATTGTAACGTCTTT+AGG 0.212897 6_4:-69517319 None:intergenic
GTAGTCTTGGCAACCTTGTC+TGG 0.232192 6_4:+69519901 Msa0994520:three_prime_UTR
CTTGTCTGGGGCACGATTAA+TGG 0.274497 6_4:+69519915 Msa0994520:three_prime_UTR
GAAGTCTACACTTTAACTAT+TGG 0.282480 6_4:+69518665 Msa0994520:CDS
ATGATGTGTTTGATTATTAT+TGG 0.294427 6_4:-69520065 None:intergenic
CCTTTGACCCTGTCAGTTCA+TGG 0.328202 6_4:-69518691 None:intergenic
TTCATATGTATTTGTAGTCT+TGG 0.344946 6_4:+69519888 Msa0994520:three_prime_UTR
GTCAGAGTATGCGGCGATCT+AGG 0.359938 6_4:-69516143 None:intergenic
TTGCGATCAGGTGGAGTTAC+GGG 0.364001 6_4:+69518772 Msa0994520:CDS
AAGTCTACACTTTAACTATT+GGG 0.381474 6_4:+69518666 Msa0994520:CDS
GCGGGAAAAGCAAGAATCTT+TGG 0.389064 6_4:+69517294 Msa0994520:five_prime_UTR
TAGTCTTGGCAACCTTGTCT+GGG 0.403501 6_4:+69519902 Msa0994520:three_prime_UTR
AATTTAATAACTTGTATAAC+CGG 0.418025 6_4:-69520106 None:intergenic
GGAAGGAAACTGTGTGGAAT+GGG 0.425540 6_4:+69517416 Msa0994520:CDS
AGAAATGATGGCCCTTGGTC+TGG 0.440363 6_4:-69518831 None:intergenic
CACCAACAACGGCTTCTTCA+AGG 0.451237 6_4:-69518717 None:intergenic
TGATCGCAACTGTCTCCATC+TGG 0.452453 6_4:-69518758 None:intergenic
TCCCTTGCCAACCTTCAAAT+CGG 0.454708 6_4:+69517489 Msa0994520:CDS
GCTTCTTCCGCCGTTCGTAA+AGG 0.460253 6_4:+69516208 Msa0994520:five_prime_UTR
CAGGTGGAGTTACGGGTTGT+TGG 0.460899 6_4:+69518779 Msa0994520:CDS
CTCCTTGAAGAAGCCGTTGT+TGG 0.463744 6_4:+69518715 Msa0994520:CDS
GACAGAGAGTGCCGATTTGA+AGG 0.467429 6_4:-69517500 None:intergenic
TGTCACACAGCTTAATTTGA+AGG 0.468733 6_4:-69519743 None:intergenic
TCTCTGTCGTCCTTCTTCTC+AGG 0.478493 6_4:+69517515 Msa0994520:CDS
TCAGATCTTACTAGAAATGA+TGG 0.481338 6_4:-69518843 None:intergenic
AGATGGAGACAGTTGCGATC+AGG 0.484734 6_4:+69518760 Msa0994520:CDS
GTTGCGATCAGGTGGAGTTA+CGG 0.486580 6_4:+69518771 Msa0994520:CDS
GAGAGTGCCGATTTGAAGGT+TGG 0.491282 6_4:-69517496 None:intergenic
TACCGATCCGGAAGTTGAAA+AGG 0.495012 6_4:+69517353 Msa0994520:five_prime_UTR
GGAGTTGTTGAACCACAGCC+TGG 0.528574 6_4:+69517456 Msa0994520:CDS
CGAGAGAGTGATCGGTAGAA+CGG 0.529551 6_4:-69516179 None:intergenic
TCAGAGTATGCGGCGATCTA+GGG 0.531517 6_4:-69516142 None:intergenic
AGTTTGTGTGTATACCGATC+CGG 0.536673 6_4:+69517341 Msa0994520:five_prime_UTR
TTACTAGAAATGATGGCCCT+TGG 0.538043 6_4:-69518836 None:intergenic
TCCCCTCTTTACCCACTGAC+CGG 0.538411 6_4:+69520087 Msa0994520:three_prime_UTR
GCAGAAACCGAGAGAGTGAT+CGG 0.538692 6_4:-69516187 None:intergenic
AGGAAGGAAACTGTGTGGAA+TGG 0.540291 6_4:+69517415 Msa0994520:CDS
CTTTATTGAGCGCGAAGCAG+TGG 0.540567 6_4:+69516107 Msa0994520:five_prime_UTR
ATTGGGTACCATGAACTGAC+AGG 0.543298 6_4:+69518683 Msa0994520:CDS
TGCCGATTTGAAGGTTGGCA+AGG 0.545614 6_4:-69517491 None:intergenic
TTGCTAGTAAGAGAAATGCT+AGG 0.551474 6_4:+69520009 Msa0994520:three_prime_UTR
AACCGGTCAGTGGGTAAAGA+GGG 0.551984 6_4:-69520089 None:intergenic
TGCTAGTAAGAGAAATGCTA+GGG 0.554311 6_4:+69520010 Msa0994520:three_prime_UTR
CATTAATCGTGCCCCAGACA+AGG 0.556391 6_4:-69519914 None:intergenic
AGAGTGGTAACCTGAGAAGA+AGG 0.560624 6_4:-69517525 None:intergenic
CGTTCTACCGATCACTCTCT+CGG 0.562206 6_4:+69516180 Msa0994520:five_prime_UTR
CCATGAACTGACAGGGTCAA+AGG 0.562919 6_4:+69518691 Msa0994520:CDS
TAACCGGTCAGTGGGTAAAG+AGG 0.564869 6_4:-69520090 None:intergenic
GCCGATTTGAAGGTTGGCAA+GGG 0.574516 6_4:-69517490 None:intergenic
GCAAGGGAATCCTGAAAACC+AGG 0.580011 6_4:-69517474 None:intergenic
AAGAAAGTCAAGCATCCAGA+TGG 0.584891 6_4:+69518743 Msa0994520:CDS
GTACAGTGCAATTGTGAGGA+AGG 0.586916 6_4:+69517399 Msa0994520:CDS
TGGAGACAGTTGCGATCAGG+TGG 0.591595 6_4:+69518763 Msa0994520:CDS
AAGAGAGTACGTATTAGTGC+GGG 0.596377 6_4:+69517276 Msa0994520:five_prime_UTR
AGTCTTGGCAACCTTGTCTG+GGG 0.598655 6_4:+69519903 Msa0994520:three_prime_UTR
GAATCCTGAAAACCAGGCTG+TGG 0.598747 6_4:-69517468 None:intergenic
AACTTGTATAACCGGTCAGT+GGG 0.600209 6_4:-69520098 None:intergenic
TAACTTGTATAACCGGTCAG+TGG 0.604796 6_4:-69520099 None:intergenic
TCGCCGCATACTCTGACGCT+CGG 0.606068 6_4:+69516149 Msa0994520:five_prime_UTR
TCAATGCACACCTTTACGAA+CGG 0.616531 6_4:-69516218 None:intergenic
ATCCGGAAGTTGAAAAGGAG+AGG 0.620407 6_4:+69517358 Msa0994520:five_prime_UTR
TGGGGCATTGTTGAACTGCA+AGG 0.624079 6_4:+69517435 Msa0994520:CDS
ACTTTCTTGAGCACCAACAA+CGG 0.632311 6_4:-69518728 None:intergenic
AAAGAGAGTACGTATTAGTG+CGG 0.646070 6_4:+69517275 Msa0994520:five_prime_UTR
TTCTGTTCAAGACCAGACCA+AGG 0.654038 6_4:+69518819 Msa0994520:CDS
GAAGGAAACTGTGTGGAATG+GGG 0.664622 6_4:+69517417 Msa0994520:CDS
AAATACATATGAATGACACA+TGG 0.665128 6_4:-69519878 None:intergenic
ATGCACACCTTTACGAACGG+CGG 0.666563 6_4:-69516215 None:intergenic
TCGTGTACAGTGCAATTGTG+AGG 0.668621 6_4:+69517395 Msa0994520:CDS
ACCGGTCAGTGGGTAAAGAG+GGG 0.669707 6_4:-69520088 None:intergenic
TCTGTTCAAGACCAGACCAA+GGG 0.678448 6_4:+69518820 Msa0994520:CDS
TTGGGTACCATGAACTGACA+GGG 0.690768 6_4:+69518684 Msa0994520:CDS
TTGTGAGGAAGGAAACTGTG+TGG 0.760163 6_4:+69517410 Msa0994520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTAAGAGAAAATTT+AGG + chr6_4:69516574-69516593 Msa0994520:intron 10.0%
!!! TTTTTTTAAGAGAAAATTTA+GGG + chr6_4:69516575-69516594 Msa0994520:intron 10.0%
!! ATAGTAAGTGATTAAATATA+GGG + chr6_4:69516639-69516658 Msa0994520:intron 15.0%
!!! AGTTTTTAACTGAAATATTA+GGG + chr6_4:69517724-69517743 Msa0994520:intron 15.0%
!! AGTGATATAAAATTAATTGT+TGG - chr6_4:69518082-69518101 None:intergenic 15.0%
!! TAACCAAAAAAAATATTACT+AGG + chr6_4:69518232-69518251 Msa0994520:intron 15.0%
!!! TTTCCTAGTAATATTTTTTT+TGG - chr6_4:69518238-69518257 None:intergenic 15.0%
!! ACAATTATTTAAAGATGATT+TGG + chr6_4:69518445-69518464 Msa0994520:intron 15.0%
!!! TGAAAAACATATTTTTATAG+GGG - chr6_4:69518889-69518908 None:intergenic 15.0%
!!! CTGAAAAACATATTTTTATA+GGG - chr6_4:69518890-69518909 None:intergenic 15.0%
!!! TCTGAAAAACATATTTTTAT+AGG - chr6_4:69518891-69518910 None:intergenic 15.0%
!!! AATTTAATAACTTGTATAAC+CGG - chr6_4:69520109-69520128 None:intergenic 15.0%
!! ATTAAACAGGTTAATTGTAA+AGG - chr6_4:69516370-69516389 None:intergenic 20.0%
!! CTGAAAAACAATTATTAAAC+AGG - chr6_4:69516383-69516402 None:intergenic 20.0%
!! ACAATTATGAGAATAATTTC+AGG + chr6_4:69516538-69516557 Msa0994520:intron 20.0%
!! CAATTATGAGAATAATTTCA+GGG + chr6_4:69516539-69516558 Msa0994520:intron 20.0%
!! TTAATCACTTACTATCAATT+TGG - chr6_4:69516634-69516653 None:intergenic 20.0%
!! GATAGTAAGTGATTAAATAT+AGG + chr6_4:69516638-69516657 Msa0994520:intron 20.0%
!! GATTATGAATATTTCAGAAA+AGG - chr6_4:69516695-69516714 None:intergenic 20.0%
!!! ATTGATTTTATCTGATTTCA+CGG + chr6_4:69516799-69516818 Msa0994520:intron 20.0%
!! AAAATGATTGAATATAACCA+CGG - chr6_4:69517057-69517076 None:intergenic 20.0%
!! ATTATTTAATAAAGTGACTC+AGG - chr6_4:69517138-69517157 None:intergenic 20.0%
!! TTATTTATTGTAACGTCTTT+AGG - chr6_4:69517322-69517341 None:intergenic 20.0%
!!! CAGTTTTTAACTGAAATATT+AGG + chr6_4:69517723-69517742 Msa0994520:intron 20.0%
!! TTCAATAAATATCAGTGATT+AGG - chr6_4:69518995-69519014 None:intergenic 20.0%
!! ATCACTGATATTTATTGAAT+AGG + chr6_4:69518996-69519015 Msa0994520:intron 20.0%
!! TATTTATTGAATAGGTTTCA+TGG + chr6_4:69519004-69519023 Msa0994520:intron 20.0%
!! GTATGTAATATAGTTAACAT+AGG - chr6_4:69519538-69519557 None:intergenic 20.0%
!! CAATTTCATGAAAAATAGTA+AGG - chr6_4:69519625-69519644 None:intergenic 20.0%
!!! CTTACTATTTTTCATGAAAT+TGG + chr6_4:69519623-69519642 Msa0994520:intron 20.0%
!! ATGATGTGTTTGATTATTAT+TGG - chr6_4:69520068-69520087 None:intergenic 20.0%
! AAATCTGTATATTGGAAGTT+TGG - chr6_4:69516245-69516264 None:intergenic 25.0%
! AGAATGCAAAATCTGTATAT+TGG - chr6_4:69516253-69516272 None:intergenic 25.0%
! CTGAAATATTCATAATCTCA+AGG + chr6_4:69516698-69516717 Msa0994520:intron 25.0%
!! TTGATTTTATCTGATTTCAC+GGG + chr6_4:69516800-69516819 Msa0994520:intron 25.0%
! TGTACTAAATAACTTATTGC+AGG - chr6_4:69516944-69516963 None:intergenic 25.0%
! AAATGATTGAATATAACCAC+GGG - chr6_4:69517056-69517075 None:intergenic 25.0%
!! ATTGTATCACTATTTTCCAA+TGG + chr6_4:69517207-69517226 Msa0994520:intron 25.0%
! GTTCTTAATTGGTAAAATGA+TGG - chr6_4:69517557-69517576 None:intergenic 25.0%
!! TCATAGTTTGAGTTCTTAAT+TGG - chr6_4:69517568-69517587 None:intergenic 25.0%
! TTCAAAGTAAGTGTTTGAAA+TGG + chr6_4:69517684-69517703 Msa0994520:intron 25.0%
!!! TTTGGTTAAGATTTTTCAAG+TGG + chr6_4:69517763-69517782 Msa0994520:intron 25.0%
!!! TTGGTTAAGATTTTTCAAGT+GGG + chr6_4:69517764-69517783 Msa0994520:intron 25.0%
!!! CTCTATTTTAAAAGCTTTGT+AGG - chr6_4:69517797-69517816 None:intergenic 25.0%
!!! TGAAACATTGTGGTTTTATA+TGG + chr6_4:69517852-69517871 Msa0994520:intron 25.0%
! TAAAGTTCCTTAGTTTAATC+CGG + chr6_4:69518051-69518070 Msa0994520:intron 25.0%
!! AAATTAATTGTTGGTCTATC+CGG - chr6_4:69518073-69518092 None:intergenic 25.0%
! TTACAAAGATAACAACAAAC+AGG - chr6_4:69518215-69518234 None:intergenic 25.0%
!! TTTTCCATATATACAAGCAT+AGG - chr6_4:69518285-69518304 None:intergenic 25.0%
!!! TTAAAGATGATTTGGTGAAA+AGG + chr6_4:69518453-69518472 Msa0994520:intron 25.0%
! GTCATAAACTCATACAAATT+CGG - chr6_4:69518558-69518577 None:intergenic 25.0%
!! TTGTTTTCCTAGTTAAGAAA+AGG - chr6_4:69518613-69518632 None:intergenic 25.0%
! AAGTCTACACTTTAACTATT+GGG + chr6_4:69518666-69518685 Msa0994520:CDS 25.0%
!!! ATTCGATATTTTTCAGACTT+TGG - chr6_4:69519342-69519361 None:intergenic 25.0%
! TGAACAATTGAAACAGAAAT+TGG + chr6_4:69519392-69519411 Msa0994520:intron 25.0%
! AATATAGTTAACATAGGAAC+AGG - chr6_4:69519532-69519551 None:intergenic 25.0%
! AAAATAGTAAGGATAGCAAA+TGG - chr6_4:69519614-69519633 None:intergenic 25.0%
!!! ACTATTTTTCATGAAATTGG+AGG + chr6_4:69519626-69519645 Msa0994520:intron 25.0%
!! TTTTCTTTAGTTGTTATCTG+TGG - chr6_4:69519695-69519714 None:intergenic 25.0%
! CTTAAACTTCTCAAAATTTC+TGG - chr6_4:69519805-69519824 None:intergenic 25.0%
!!! CAGAAATTTTGAGAAGTTTA+AGG + chr6_4:69519803-69519822 Msa0994520:three_prime_UTR 25.0%
! AAATACATATGAATGACACA+TGG - chr6_4:69519881-69519900 None:intergenic 25.0%
! TTCATATGTATTTGTAGTCT+TGG + chr6_4:69519888-69519907 Msa0994520:three_prime_UTR 25.0%
TTACAAAGTCAAGACCAATT+TGG - chr6_4:69516417-69516436 None:intergenic 30.0%
TTTGTAAGTAACTCTCAAAC+AGG + chr6_4:69516430-69516449 Msa0994520:intron 30.0%
ACTCTAATAAACTGTCAAGT+TGG - chr6_4:69516666-69516685 None:intergenic 30.0%
TACAAAGTCAAGGACTAATT+TGG + chr6_4:69516739-69516758 Msa0994520:intron 30.0%
! TGATTTTATCTGATTTCACG+GGG + chr6_4:69516801-69516820 Msa0994520:intron 30.0%
! TTTTCAAATAGTGACTGTAG+TGG + chr6_4:69516970-69516989 Msa0994520:intron 30.0%
! ATCACTATTTTCCAATGGAT+CGG + chr6_4:69517212-69517231 Msa0994520:intron 30.0%
! AAAATGATGGAAAAGAAGAG+TGG - chr6_4:69517544-69517563 None:intergenic 30.0%
CTCATCTCTATCATGATTAT+TGG + chr6_4:69517601-69517620 Msa0994520:intron 30.0%
GGTTTGAATAATCGTCAATT+TGG + chr6_4:69517745-69517764 Msa0994520:intron 30.0%
!!! TGGTTAAGATTTTTCAAGTG+GGG + chr6_4:69517765-69517784 Msa0994520:intron 30.0%
GTGTTAAACTTGAAACATTG+TGG + chr6_4:69517842-69517861 Msa0994520:intron 30.0%
AGGAATAAACTTACTGATGT+GGG + chr6_4:69517910-69517929 Msa0994520:intron 30.0%
TTATCTGTCCAAAATCTTGA+AGG + chr6_4:69518018-69518037 Msa0994520:intron 30.0%
TATCTGTCCAAAATCTTGAA+GGG + chr6_4:69518019-69518038 Msa0994520:intron 30.0%
! TTAAGAACCCTTCAAGATTT+TGG - chr6_4:69518029-69518048 None:intergenic 30.0%
ACTCTTATAGTTTAAGGACA+CGG - chr6_4:69518352-69518371 None:intergenic 30.0%
ATCAGGACTCTTATAGTTTA+AGG - chr6_4:69518358-69518377 None:intergenic 30.0%
! GACATAGTCTTTTCATAGAT+TGG + chr6_4:69518575-69518594 Msa0994520:intron 30.0%
GAAGTCTACACTTTAACTAT+TGG + chr6_4:69518665-69518684 Msa0994520:CDS 30.0%
TCAGATCTTACTAGAAATGA+TGG - chr6_4:69518846-69518865 None:intergenic 30.0%
TAAATATCAGTGATTAGGAC+TGG - chr6_4:69518990-69519009 None:intergenic 30.0%
AATCAAGAAAACTTAACCGT+TGG + chr6_4:69519203-69519222 Msa0994520:intron 30.0%
TAATAAGCACAGGACTAAAA+AGG - chr6_4:69519372-69519391 None:intergenic 30.0%
CAATTGTTCATAATAAGCAC+AGG - chr6_4:69519382-69519401 None:intergenic 30.0%
! GAAACTCACTGTATATTTTC+TGG + chr6_4:69519481-69519500 Msa0994520:intron 30.0%
CTGTTTATTACGTGTATTTG+TGG + chr6_4:69519506-69519525 Msa0994520:intron 30.0%
TTGAAAACACAAGGAAAAGA+AGG - chr6_4:69519669-69519688 None:intergenic 30.0%
TGCTTGTAGTGTAGAAAAAA+GGG - chr6_4:69519841-69519860 None:intergenic 30.0%
TTGCTTGTAGTGTAGAAAAA+AGG - chr6_4:69519842-69519861 None:intergenic 30.0%
CAGATAGTAAACCACCAAAT+TGG + chr6_4:69516400-69516419 Msa0994520:intron 35.0%
TATTAGTCCTTTCAGAGTCA+TGG + chr6_4:69516514-69516533 Msa0994520:intron 35.0%
AATTGTTCCATGACTCTGAA+AGG - chr6_4:69516524-69516543 None:intergenic 35.0%
AAAGTCAAGGACTAATTTGG+TGG + chr6_4:69516742-69516761 Msa0994520:intron 35.0%
AGCTATAGTGTAGCAGAATT+AGG + chr6_4:69516903-69516922 Msa0994520:intron 35.0%
CAATATTCAAACCGATCCAT+TGG - chr6_4:69517226-69517245 None:intergenic 35.0%
TTTCCTAACCATGGAAATAG+GGG - chr6_4:69517252-69517271 None:intergenic 35.0%
CTTTCCTAACCATGGAAATA+GGG - chr6_4:69517253-69517272 None:intergenic 35.0%
CTATTTCCATGGTTAGGAAA+GGG + chr6_4:69517252-69517271 Msa0994520:intron 35.0%
! AAAGAGAGTACGTATTAGTG+CGG + chr6_4:69517275-69517294 Msa0994520:five_prime_UTR 35.0%
!! AGATTGAATGTTTGATAGCG+AGG + chr6_4:69517890-69517909 Msa0994520:intron 35.0%
GAGGAATAAACTTACTGATG+TGG + chr6_4:69517909-69517928 Msa0994520:intron 35.0%
GTGTCCTATGCTTGTATATA+TGG + chr6_4:69518278-69518297 Msa0994520:intron 35.0%
TTATAGTTTAAGGACACGGT+TGG - chr6_4:69518348-69518367 None:intergenic 35.0%
TTCTGTATGGAAGCTAAATC+AGG - chr6_4:69518375-69518394 None:intergenic 35.0%
TAGTTAAGAAAAGGTGACAG+TGG - chr6_4:69518604-69518623 None:intergenic 35.0%
! ACTGTCACCTTTTCTTAACT+AGG + chr6_4:69518603-69518622 Msa0994520:intron 35.0%
!!! TGTCCATTGTGTTGTTTTTC+AGG + chr6_4:69518643-69518662 Msa0994520:intron 35.0%
CTTCCTGAAAAACAACACAA+TGG - chr6_4:69518649-69518668 None:intergenic 35.0%
GAATACAAGTTGTGTTGTGT+AGG + chr6_4:69518921-69518940 Msa0994520:intron 35.0%
TACTTAGGCCAATATGGAAA+CGG - chr6_4:69519138-69519157 None:intergenic 35.0%
AGTTGTTCAATCTTCTCCAA+CGG - chr6_4:69519222-69519241 None:intergenic 35.0%
! CTTTGGATTTTCTACTGTCA+CGG - chr6_4:69519325-69519344 None:intergenic 35.0%
TGTAGACAAGACCTATTGTT+AGG + chr6_4:69519459-69519478 Msa0994520:intron 35.0%
ACAGTGAGTTTCCTAACAAT+AGG - chr6_4:69519473-69519492 None:intergenic 35.0%
AGAAGGATAACATTAGCAGT+GGG - chr6_4:69519652-69519671 None:intergenic 35.0%
AAGAAGGATAACATTAGCAG+TGG - chr6_4:69519653-69519672 None:intergenic 35.0%
! TGTCACACAGCTTAATTTGA+AGG - chr6_4:69519746-69519765 None:intergenic 35.0%
TTGCTAGTAAGAGAAATGCT+AGG + chr6_4:69520009-69520028 Msa0994520:three_prime_UTR 35.0%
TGCTAGTAAGAGAAATGCTA+GGG + chr6_4:69520010-69520029 Msa0994520:three_prime_UTR 35.0%
TCAATGCACACCTTTACGAA+CGG - chr6_4:69516221-69516240 None:intergenic 40.0%
! TTTTTCTCGCTTCGATGCTT+AGG + chr6_4:69516283-69516302 Msa0994520:intron 40.0%
CAAAGTCAAGACCAATTTGG+TGG - chr6_4:69516414-69516433 None:intergenic 40.0%
! CAGAGTGTTCTACAAAGTCA+AGG + chr6_4:69516729-69516748 Msa0994520:intron 40.0%
TTTATCTGATTTCACGGGGA+TGG + chr6_4:69516805-69516824 Msa0994520:intron 40.0%
TTATCTGATTTCACGGGGAT+GGG + chr6_4:69516806-69516825 Msa0994520:intron 40.0%
CATTGAAACACATTTGCCGT+TGG - chr6_4:69516871-69516890 None:intergenic 40.0%
!! GCTATAGCATTGATGCATAG+CGG + chr6_4:69516994-69517013 Msa0994520:intron 40.0%
GCTCAAAATCTATGTGGCAT+TGG - chr6_4:69517116-69517135 None:intergenic 40.0%
ACTCAGGCTCAAAATCTATG+TGG - chr6_4:69517122-69517141 None:intergenic 40.0%
TATTGACACCCCTATTTCCA+TGG + chr6_4:69517241-69517260 Msa0994520:intron 40.0%
CCTTTCCTAACCATGGAAAT+AGG - chr6_4:69517254-69517273 None:intergenic 40.0%
CCTATTTCCATGGTTAGGAA+AGG + chr6_4:69517251-69517270 Msa0994520:intron 40.0%
! AAGAGAGTACGTATTAGTGC+GGG + chr6_4:69517276-69517295 Msa0994520:five_prime_UTR 40.0%
AGTTTGTGTGTATACCGATC+CGG + chr6_4:69517341-69517360 Msa0994520:five_prime_UTR 40.0%
GGTCTATCCGGATTAAACTA+AGG - chr6_4:69518061-69518080 None:intergenic 40.0%
TGCAGGAACTTTCTACCTTA+GGG + chr6_4:69518147-69518166 Msa0994520:intron 40.0%
ACTTTCTTGAGCACCAACAA+CGG - chr6_4:69518731-69518750 None:intergenic 40.0%
AAGAAAGTCAAGCATCCAGA+TGG + chr6_4:69518743-69518762 Msa0994520:CDS 40.0%
TTACTAGAAATGATGGCCCT+TGG - chr6_4:69518839-69518858 None:intergenic 40.0%
! ATCTACTGCCGTTTCCATAT+TGG + chr6_4:69519127-69519146 Msa0994520:intron 40.0%
! TTCCATATTGGCCTAAGTAG+AGG + chr6_4:69519139-69519158 Msa0994520:intron 40.0%
CACATTTCAGTCCTCTACTT+AGG - chr6_4:69519153-69519172 None:intergenic 40.0%
CTAAGTAGAGGACTGAAATG+TGG + chr6_4:69519151-69519170 Msa0994520:intron 40.0%
TTGATTGTGCGACTGTATGA+GGG - chr6_4:69519189-69519208 None:intergenic 40.0%
CTGTGGTTCTTGAAAACACA+AGG - chr6_4:69519678-69519697 None:intergenic 40.0%
GGCACGATTAATGGAAAAAG+TGG + chr6_4:69519924-69519943 Msa0994520:three_prime_UTR 40.0%
AACTTGTATAACCGGTCAGT+GGG - chr6_4:69520101-69520120 None:intergenic 40.0%
! TAACTTGTATAACCGGTCAG+TGG - chr6_4:69520102-69520121 None:intergenic 40.0%
TATCTGATTTCACGGGGATG+GGG + chr6_4:69516807-69516826 Msa0994520:intron 45.0%
ACTTACTGCCTATGTAGCAC+CGG + chr6_4:69516841-69516860 Msa0994520:intron 45.0%
ACACCCCTATTTCCATGGTT+AGG + chr6_4:69517246-69517265 Msa0994520:intron 45.0%
CTCTTTCCCTTTCCTAACCA+TGG - chr6_4:69517261-69517280 None:intergenic 45.0%
GCGGGAAAAGCAAGAATCTT+TGG + chr6_4:69517294-69517313 Msa0994520:five_prime_UTR 45.0%
TACCGATCCGGAAGTTGAAA+AGG + chr6_4:69517353-69517372 Msa0994520:five_prime_UTR 45.0%
! CTCCTTTTCAACTTCCGGAT+CGG - chr6_4:69517358-69517377 None:intergenic 45.0%
ATCCGGAAGTTGAAAAGGAG+AGG + chr6_4:69517358-69517377 Msa0994520:five_prime_UTR 45.0%
! GTCCTCTCCTTTTCAACTTC+CGG - chr6_4:69517363-69517382 None:intergenic 45.0%
TCGTGTACAGTGCAATTGTG+AGG + chr6_4:69517395-69517414 Msa0994520:CDS 45.0%
GTACAGTGCAATTGTGAGGA+AGG + chr6_4:69517399-69517418 Msa0994520:CDS 45.0%
TTGTGAGGAAGGAAACTGTG+TGG + chr6_4:69517410-69517429 Msa0994520:CDS 45.0%
AGGAAGGAAACTGTGTGGAA+TGG + chr6_4:69517415-69517434 Msa0994520:CDS 45.0%
GGAAGGAAACTGTGTGGAAT+GGG + chr6_4:69517416-69517435 Msa0994520:CDS 45.0%
GAAGGAAACTGTGTGGAATG+GGG + chr6_4:69517417-69517436 Msa0994520:CDS 45.0%
TCCCTTGCCAACCTTCAAAT+CGG + chr6_4:69517489-69517508 Msa0994520:CDS 45.0%
AGAGTGGTAACCTGAGAAGA+AGG - chr6_4:69517528-69517547 None:intergenic 45.0%
! ATTGACAGACCTGCAGCTTT+TGG - chr6_4:69518110-69518129 None:intergenic 45.0%
CTGCAGGAACTTTCTACCTT+AGG + chr6_4:69518146-69518165 Msa0994520:intron 45.0%
CACAAGCAAACTACTCCCTA+AGG - chr6_4:69518165-69518184 None:intergenic 45.0%
!! AGGGAGTAGTTTGCTTGTGT+TGG + chr6_4:69518166-69518185 Msa0994520:intron 45.0%
CCACGTCATGCAATTCTGTA+TGG - chr6_4:69518388-69518407 None:intergenic 45.0%
CCATACAGAATTGCATGACG+TGG + chr6_4:69518385-69518404 Msa0994520:intron 45.0%
CATGACGTGGTTATCGATCT+TGG + chr6_4:69518398-69518417 Msa0994520:intron 45.0%
ACGTGGTTATCGATCTTGGT+TGG + chr6_4:69518402-69518421 Msa0994520:intron 45.0%
ATTGGGTACCATGAACTGAC+AGG + chr6_4:69518683-69518702 Msa0994520:CDS 45.0%
TTGGGTACCATGAACTGACA+GGG + chr6_4:69518684-69518703 Msa0994520:CDS 45.0%
TTCTGTTCAAGACCAGACCA+AGG + chr6_4:69518819-69518838 Msa0994520:CDS 45.0%
TCTGTTCAAGACCAGACCAA+GGG + chr6_4:69518820-69518839 Msa0994520:CDS 45.0%
GTCCTCTACTTAGGCCAATA+TGG - chr6_4:69519144-69519163 None:intergenic 45.0%
AGTAGAGGACTGAAATGTGG+AGG + chr6_4:69519154-69519173 Msa0994520:intron 45.0%
CTTGATTGTGCGACTGTATG+AGG - chr6_4:69519190-69519209 None:intergenic 45.0%
TACGCAATTGATGCGCTTAC+TGG + chr6_4:69519555-69519574 Msa0994520:intron 45.0%
! TAGTCTTGGCAACCTTGTCT+GGG + chr6_4:69519902-69519921 Msa0994520:three_prime_UTR 45.0%
CTTTATTGAGCGCGAAGCAG+TGG + chr6_4:69516107-69516126 Msa0994520:five_prime_UTR 50.0%
TCAGAGTATGCGGCGATCTA+GGG - chr6_4:69516145-69516164 None:intergenic 50.0%
CGAGAGAGTGATCGGTAGAA+CGG - chr6_4:69516182-69516201 None:intergenic 50.0%
CGTTCTACCGATCACTCTCT+CGG + chr6_4:69516180-69516199 Msa0994520:five_prime_UTR 50.0%
GCAGAAACCGAGAGAGTGAT+CGG - chr6_4:69516190-69516209 None:intergenic 50.0%
ATGCACACCTTTACGAACGG+CGG - chr6_4:69516218-69516237 None:intergenic 50.0%
!! TTGGTGTACCGGTGCTACAT+AGG - chr6_4:69516852-69516871 None:intergenic 50.0%
!! ATGTAGCACCGGTACACCAA+CGG + chr6_4:69516852-69516871 Msa0994520:intron 50.0%
CACATTTGCCGTTGGTGTAC+CGG - chr6_4:69516863-69516882 None:intergenic 50.0%
! GTGTTCTCAAGCACGGATTG+TGG - chr6_4:69517032-69517051 None:intergenic 50.0%
!! TGGGGCATTGTTGAACTGCA+AGG + chr6_4:69517435-69517454 Msa0994520:CDS 50.0%
!! TGAACCACAGCCTGGTTTTC+AGG + chr6_4:69517464-69517483 Msa0994520:CDS 50.0%
GAATCCTGAAAACCAGGCTG+TGG - chr6_4:69517471-69517490 None:intergenic 50.0%
GCAAGGGAATCCTGAAAACC+AGG - chr6_4:69517477-69517496 None:intergenic 50.0%
!! GCCGATTTGAAGGTTGGCAA+GGG - chr6_4:69517493-69517512 None:intergenic 50.0%
!! TGCCGATTTGAAGGTTGGCA+AGG - chr6_4:69517494-69517513 None:intergenic 50.0%
!! GAGAGTGCCGATTTGAAGGT+TGG - chr6_4:69517499-69517518 None:intergenic 50.0%
!! GACAGAGAGTGCCGATTTGA+AGG - chr6_4:69517503-69517522 None:intergenic 50.0%
TCTCTGTCGTCCTTCTTCTC+AGG + chr6_4:69517515-69517534 Msa0994520:CDS 50.0%
CACTTCTGTCCAAAAGCTGC+AGG + chr6_4:69518098-69518117 Msa0994520:intron 50.0%
CCTTTGACCCTGTCAGTTCA+TGG - chr6_4:69518694-69518713 None:intergenic 50.0%
CCATGAACTGACAGGGTCAA+AGG + chr6_4:69518691-69518710 Msa0994520:CDS 50.0%
CTCCTTGAAGAAGCCGTTGT+TGG + chr6_4:69518715-69518734 Msa0994520:CDS 50.0%
CACCAACAACGGCTTCTTCA+AGG - chr6_4:69518720-69518739 None:intergenic 50.0%
TGATCGCAACTGTCTCCATC+TGG - chr6_4:69518761-69518780 None:intergenic 50.0%
AGATGGAGACAGTTGCGATC+AGG + chr6_4:69518760-69518779 Msa0994520:CDS 50.0%
GTTGCGATCAGGTGGAGTTA+CGG + chr6_4:69518771-69518790 Msa0994520:CDS 50.0%
TTGCGATCAGGTGGAGTTAC+GGG + chr6_4:69518772-69518791 Msa0994520:CDS 50.0%
AGAAATGATGGCCCTTGGTC+TGG - chr6_4:69518834-69518853 None:intergenic 50.0%
GCGACTGTATGAGGGAATTC+AGG - chr6_4:69519181-69519200 None:intergenic 50.0%
! GTAGTCTTGGCAACCTTGTC+TGG + chr6_4:69519901-69519920 Msa0994520:three_prime_UTR 50.0%
AGTCTTGGCAACCTTGTCTG+GGG + chr6_4:69519903-69519922 Msa0994520:three_prime_UTR 50.0%
CATTAATCGTGCCCCAGACA+AGG - chr6_4:69519917-69519936 None:intergenic 50.0%
! CTTGTCTGGGGCACGATTAA+TGG + chr6_4:69519915-69519934 Msa0994520:three_prime_UTR 50.0%
AACCGGTCAGTGGGTAAAGA+GGG - chr6_4:69520092-69520111 None:intergenic 50.0%
TAACCGGTCAGTGGGTAAAG+AGG - chr6_4:69520093-69520112 None:intergenic 50.0%
GTCAGAGTATGCGGCGATCT+AGG - chr6_4:69516146-69516165 None:intergenic 55.0%
GCTTCTTCCGCCGTTCGTAA+AGG + chr6_4:69516208-69516227 Msa0994520:five_prime_UTR 55.0%
TTTCACGGGGATGGGGAATG+AGG + chr6_4:69516814-69516833 Msa0994520:intron 55.0%
TTCACGGGGATGGGGAATGA+GGG + chr6_4:69516815-69516834 Msa0994520:intron 55.0%
!! CACGGGAGTGTTCTCAAGCA+CGG - chr6_4:69517039-69517058 None:intergenic 55.0%
GGAGTTGTTGAACCACAGCC+TGG + chr6_4:69517456-69517475 Msa0994520:CDS 55.0%
TGGAGACAGTTGCGATCAGG+TGG + chr6_4:69518763-69518782 Msa0994520:CDS 55.0%
! CAGGTGGAGTTACGGGTTGT+TGG + chr6_4:69518779-69518798 Msa0994520:CDS 55.0%
TCCCCTCTTTACCCACTGAC+CGG + chr6_4:69520087-69520106 Msa0994520:three_prime_UTR 55.0%
ACCGGTCAGTGGGTAAAGAG+GGG - chr6_4:69520091-69520110 None:intergenic 55.0%
TCGCCGCATACTCTGACGCT+CGG + chr6_4:69516149-69516168 Msa0994520:five_prime_UTR 60.0%
GCTCCGAGCGTCAGAGTATG+CGG - chr6_4:69516155-69516174 None:intergenic 60.0%
CGTGCTTGAGAACACTCCCG+TGG + chr6_4:69517037-69517056 Msa0994520:intron 60.0%
CATCTTCGCAGCGACGCTGC+AGG + chr6_4:69518130-69518149 Msa0994520:intron 65.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 69516076 69520139 69516076 ID=Msa0994520;Name=Msa0994520
chr6_4 mRNA 69516076 69520139 69516076 ID=Msa0994520-mRNA-1;Parent=Msa0994520;Name=Msa0994520-mRNA-1;_AED=0.07;_eAED=0.07;_QI=273|1|1|1|0.33|0|4|385|134
chr6_4 exon 69516076 69516229 69516076 ID=Msa0994520-mRNA-1:exon:12788;Parent=Msa0994520-mRNA-1
chr6_4 exon 69517268 69517536 69517268 ID=Msa0994520-mRNA-1:exon:12789;Parent=Msa0994520-mRNA-1
chr6_4 exon 69518665 69518854 69518665 ID=Msa0994520-mRNA-1:exon:12790;Parent=Msa0994520-mRNA-1
chr6_4 exon 69519690 69520139 69519690 ID=Msa0994520-mRNA-1:exon:12791;Parent=Msa0994520-mRNA-1
chr6_4 five_prime_UTR 69516076 69516229 69516076 ID=Msa0994520-mRNA-1:five_prime_utr;Parent=Msa0994520-mRNA-1
chr6_4 five_prime_UTR 69517268 69517386 69517268 ID=Msa0994520-mRNA-1:five_prime_utr;Parent=Msa0994520-mRNA-1
chr6_4 CDS 69517387 69517536 69517387 ID=Msa0994520-mRNA-1:cds;Parent=Msa0994520-mRNA-1
chr6_4 CDS 69518665 69518854 69518665 ID=Msa0994520-mRNA-1:cds;Parent=Msa0994520-mRNA-1
chr6_4 CDS 69519690 69519754 69519690 ID=Msa0994520-mRNA-1:cds;Parent=Msa0994520-mRNA-1
chr6_4 three_prime_UTR 69519755 69520139 69519755 ID=Msa0994520-mRNA-1:three_prime_utr;Parent=Msa0994520-mRNA-1
Gene Sequence

>Msa0994520

ATGCAGATTCGTGTACAGTGCAATTGTGAGGAAGGAAACTGTGTGGAATGGGGCATTGTTGAACTGCAAGGAGTTGTTGAACCACAGCCTGGTTTTCAGGATTCCCTTGCCAACCTTCAAATCGGCACTCTCTGTCGTCCTTCTTCTCAGGAAGTCTACACTTTAACTATTGGGTACCATGAACTGACAGGGTCAAAGGTCTCCTTGAAGAAGCCGTTGTTGGTGCTCAAGAAAGTCAAGCATCCAGATGGAGACAGTTGCGATCAGGTGGAGTTACGGGTTGTTGGAATCATTCGACAGAAAATTCTGTTCAAGACCAGACCAAGGGCCATCATTTCTAAACCACAGATAACAACTAAAGAAAAACAAAAGTCTGTTATGACAGACTCTGCTCCTTCAAATTAA
Protein sequence

>Msa0994520

MQIRVQCNCEEGNCVEWGIVELQGVVEPQPGFQDSLANLQIGTLCRPSSQEVYTLTIGYHELTGSKVSLKKPLLVLKKVKHPDGDSCDQVELRVVGIIRQKILFKTRPRAIISKPQITTKEKQKSVMTDSAPSN*