Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1000130 | KEH27158.1 | 81.176 | 85 | 16 | 0 | 18 | 102 | 144 | 228 | 5.88e-40 | 144 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1000130 | G8A0M6 | 81.176 | 85 | 16 | 0 | 18 | 102 | 144 | 228 | 2.81e-40 | 144 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1000130 | Msa1410640 | 0.825493 | 4.548880e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1000130 | MtrunA17_Chr6g0486181 | 81.176 | 85 | 16 | 0 | 18 | 102 | 144 | 228 | 5.41e-44 | 144 |
| Msa1000130 | MtrunA17_Chr6g0486141 | 50.617 | 81 | 40 | 0 | 21 | 101 | 148 | 228 | 2.97e-20 | 84.0 |
| Msa1000130 | MtrunA17_Chr6g0486171 | 43.158 | 95 | 53 | 1 | 1 | 94 | 114 | 208 | 4.68e-16 | 71.6 |
| Msa1000130 | MtrunA17_Chr6g0486151 | 51.316 | 76 | 35 | 2 | 20 | 94 | 165 | 239 | 8.29e-16 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGGAAATAAAATAGATAT+TGG | 0.266231 | 6_4:-82840212 | Msa1000130:CDS |
| ATTAAACAAGTTTCTAGAAC+AGG | 0.359747 | 6_4:-82840092 | Msa1000130:CDS |
| AATAGATATTGGACTTTCAA+TGG | 0.360085 | 6_4:-82840201 | Msa1000130:CDS |
| TTCTTTCATGGAGGAGGAAG+GGG | 0.389696 | 6_4:-82840330 | None:intergenic |
| GTTGAAGAAGGGTGTTGAAC+AGG | 0.413754 | 6_4:+82840128 | None:intergenic |
| TGTGCCACAGTAACTTTGTA+AGG | 0.430676 | 6_4:+82840266 | None:intergenic |
| AAATAATATGTTATTTGATA+CGG | 0.464432 | 6_4:+82840166 | None:intergenic |
| AAGTTTCTAGAACAGGGTAT+TGG | 0.464513 | 6_4:-82840085 | Msa1000130:CDS |
| ATACGAAGCAATGTTGAAGA+AGG | 0.501068 | 6_4:+82840116 | None:intergenic |
| ATATTATTTGTTTCAGCGGA+GGG | 0.515871 | 6_4:-82840152 | Msa1000130:CDS |
| CATGAGCATGATTCTGACAC+TGG | 0.533230 | 6_4:-82840044 | Msa1000130:CDS |
| TAACATATTATTTGTTTCAG+CGG | 0.536777 | 6_4:-82840156 | Msa1000130:CDS |
| TTCTAGAACAGGGTATTGGA+AGG | 0.541064 | 6_4:-82840081 | Msa1000130:CDS |
| TTTCTTTCATGGAGGAGGAA+GGG | 0.543712 | 6_4:-82840331 | None:intergenic |
| CATATTATTTGTTTCAGCGG+AGG | 0.560909 | 6_4:-82840153 | Msa1000130:CDS |
| TCTAGAACAGGGTATTGGAA+GGG | 0.578826 | 6_4:-82840080 | Msa1000130:CDS |
| TACGAAGCAATGTTGAAGAA+GGG | 0.616487 | 6_4:+82840117 | None:intergenic |
| ATGAGCATGATTCTGACACT+GGG | 0.630580 | 6_4:-82840043 | Msa1000130:CDS |
| TTAAACAAGTTTCTAGAACA+GGG | 0.653611 | 6_4:-82840091 | Msa1000130:CDS |
| TTGAACAGAATAGCATGCAG+AGG | 0.680886 | 6_4:-82840296 | Msa1000130:CDS |
| GAATCCTTACAAAGTTACTG+TGG | 0.689365 | 6_4:-82840270 | Msa1000130:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATAATATGTTATTTGATA+CGG | + | chr6_4:82840152-82840171 | None:intergenic | 10.0% |
| !! | AGTGGAAATAAAATAGATAT+TGG | - | chr6_4:82840103-82840122 | Msa1000130:CDS | 20.0% |
| !! | TAACATATTATTTGTTTCAG+CGG | - | chr6_4:82840159-82840178 | Msa1000130:CDS | 20.0% |
| !! | CCACTAAAATCATTTTCATT+GGG | + | chr6_4:82840088-82840107 | None:intergenic | 25.0% |
| !! | TCCACTAAAATCATTTTCAT+TGG | + | chr6_4:82840089-82840108 | None:intergenic | 25.0% |
| ! | AATAGATATTGGACTTTCAA+TGG | - | chr6_4:82840114-82840133 | Msa1000130:CDS | 25.0% |
| !!! | ATTAAACAAGTTTCTAGAAC+AGG | - | chr6_4:82840223-82840242 | Msa1000130:CDS | 25.0% |
| !!! | TTAAACAAGTTTCTAGAACA+GGG | - | chr6_4:82840224-82840243 | Msa1000130:CDS | 25.0% |
| ! | CCCAATGAAAATGATTTTAG+TGG | - | chr6_4:82840085-82840104 | Msa1000130:CDS | 30.0% |
| ATATTATTTGTTTCAGCGGA+GGG | - | chr6_4:82840163-82840182 | Msa1000130:CDS | 30.0% | |
| GAATCCTTACAAAGTTACTG+TGG | - | chr6_4:82840045-82840064 | Msa1000130:CDS | 35.0% | |
| CATATTATTTGTTTCAGCGG+AGG | - | chr6_4:82840162-82840181 | Msa1000130:CDS | 35.0% | |
| TACGAAGCAATGTTGAAGAA+GGG | + | chr6_4:82840201-82840220 | None:intergenic | 35.0% | |
| ATACGAAGCAATGTTGAAGA+AGG | + | chr6_4:82840202-82840221 | None:intergenic | 35.0% | |
| !! | AAGTTTCTAGAACAGGGTAT+TGG | - | chr6_4:82840230-82840249 | Msa1000130:CDS | 35.0% |
| TTGAACAGAATAGCATGCAG+AGG | - | chr6_4:82840019-82840038 | Msa1000130:CDS | 40.0% | |
| TGTGCCACAGTAACTTTGTA+AGG | + | chr6_4:82840052-82840071 | None:intergenic | 40.0% | |
| !! | TTCTAGAACAGGGTATTGGA+AGG | - | chr6_4:82840234-82840253 | Msa1000130:CDS | 40.0% |
| !! | TCTAGAACAGGGTATTGGAA+GGG | - | chr6_4:82840235-82840254 | Msa1000130:CDS | 40.0% |
| ATGAGCATGATTCTGACACT+GGG | - | chr6_4:82840272-82840291 | Msa1000130:CDS | 40.0% | |
| !! | GTTGAAGAAGGGTGTTGAAC+AGG | + | chr6_4:82840190-82840209 | None:intergenic | 45.0% |
| CATGAGCATGATTCTGACAC+TGG | - | chr6_4:82840271-82840290 | Msa1000130:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 82839992 | 82840345 | 82839992 | ID=Msa1000130;Name=Msa1000130 |
| chr6_4 | mRNA | 82839992 | 82840345 | 82839992 | ID=Msa1000130-mRNA-1;Parent=Msa1000130;Name=Msa1000130-mRNA-1;_AED=0.38;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|117 |
| chr6_4 | exon | 82839992 | 82840345 | 82839992 | ID=Msa1000130-mRNA-1:exon:15965;Parent=Msa1000130-mRNA-1 |
| chr6_4 | CDS | 82839992 | 82840345 | 82839992 | ID=Msa1000130-mRNA-1:cds;Parent=Msa1000130-mRNA-1 |
| Gene Sequence |
| Protein sequence |
