Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1410640 | G8A0M6 | 46.087 | 230 | 50 | 5 | 1 | 158 | 1 | 228 | 8.31e-49 | 169 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1410620 | Msa1410640 | 0.865745 | 4.725387e-65 | -8.615850e-47 |
| Msa1410640 | Msa1414740 | 0.817109 | 3.910091e-52 | -8.615850e-47 |
| Msa0657970 | Msa1410640 | 0.814022 | 1.903689e-51 | -8.615850e-47 |
| Msa0693380 | Msa1410640 | 0.806479 | 8.065087e-50 | -8.615850e-47 |
| Msa0217400 | Msa1410640 | 0.809962 | 1.459567e-50 | -8.615850e-47 |
| Msa1228210 | Msa1410640 | 0.817536 | 3.134096e-52 | -8.615850e-47 |
| Msa1271300 | Msa1410640 | 0.811534 | 6.673523e-51 | -8.615850e-47 |
| Msa1283860 | Msa1410640 | 0.815387 | 9.489755e-52 | -8.615850e-47 |
| Msa1324780 | Msa1410640 | 0.829272 | 5.647474e-55 | -8.615850e-47 |
| Msa0437990 | Msa1410640 | 0.801884 | 7.293770e-49 | -8.615850e-47 |
| Msa1000050 | Msa1410640 | 0.807801 | 4.232282e-50 | -8.615850e-47 |
| Msa1000130 | Msa1410640 | 0.825493 | 4.548880e-54 | -8.615850e-47 |
| Msa1036390 | Msa1410640 | 0.803806 | 2.924288e-49 | -8.615850e-47 |
| Msa1082500 | Msa1410640 | 0.820808 | 5.638485e-53 | -8.615850e-47 |
| Msa1167220 | Msa1410640 | 0.800290 | 1.544606e-48 | -8.615850e-47 |
| Msa0484780 | Msa1410640 | 0.800858 | 1.183385e-48 | -8.615850e-47 |
| Msa0880140 | Msa1410640 | 0.802215 | 6.234504e-49 | -8.615850e-47 |
| Msa0926950 | Msa1410640 | 0.822770 | 1.982692e-53 | -8.615850e-47 |
| Msa0959770 | Msa1410640 | 0.806672 | 7.340519e-50 | -8.615850e-47 |
| Msa0965570 | Msa1410640 | 0.853461 | 2.354282e-61 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1410640 | MtrunA17_Chr6g0486181 | 46.087 | 230 | 50 | 5 | 1 | 158 | 1 | 228 | 1.60e-52 | 169 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAAGTCGCGGATTCTTTA+AGG | 0.149783 | tig0023681:+44721 | Msa1410640:CDS |
| GATATTAAGGATAAGAGTTT+AGG | 0.306646 | tig0023681:+44470 | Msa1410640:CDS |
| ATTAAACAAGTTTCTAGAAC+AGG | 0.359156 | tig0023681:+44995 | Msa1410640:CDS |
| AAGTTTCTAGAACAGGGTTT+TGG | 0.363927 | tig0023681:+45002 | Msa1410640:CDS |
| TTCTTTCATGGAGGAGGAAG+GGG | 0.389696 | tig0023681:+44755 | Msa1410640:CDS |
| AAAGAATCCGCGACTTTCAT+AGG | 0.395410 | tig0023681:-44717 | None:intergenic |
| TACATCGAATAAAGTACTTT+AGG | 0.397763 | tig0023681:+45224 | Msa1410640:three_prime_UTR |
| GTTGAAGAAGGGTGTTGAAC+AGG | 0.413754 | tig0023681:-44959 | None:intergenic |
| TGAATTAAGTTCATGTAAAG+TGG | 0.425996 | tig0023681:-45255 | None:intergenic |
| TCTATCAAATACTCTAGTTA+AGG | 0.433105 | tig0023681:-44549 | None:intergenic |
| AGTAATAGGAATATAAAAGT+TGG | 0.445872 | tig0023681:+44416 | Msa1410640:CDS |
| ATACGAAGCAATGTTGAAGA+AGG | 0.501068 | tig0023681:-44971 | None:intergenic |
| GATAGAGATGGTAGCACTCA+AGG | 0.505801 | tig0023681:+44566 | Msa1410640:CDS |
| CATGAATATAAAATTATCGA+TGG | 0.508016 | tig0023681:+45157 | Msa1410640:three_prime_UTR |
| ACTAGAGTATTTGATAGAGA+TGG | 0.527025 | tig0023681:+44554 | Msa1410640:CDS |
| CATGAGCATGATTCTGACAC+TGG | 0.533230 | tig0023681:+45043 | Msa1410640:CDS |
| TTTCTTTCATGGAGGAGGAA+GGG | 0.543712 | tig0023681:+44754 | Msa1410640:CDS |
| AAGTGAAATACATAATTGAC+GGG | 0.549424 | tig0023681:+44510 | Msa1410640:CDS |
| GAAGTGAAATACATAATTGA+CGG | 0.553540 | tig0023681:+44509 | Msa1410640:CDS |
| ATGAATATAAAATTATCGAT+GGG | 0.565717 | tig0023681:+45158 | Msa1410640:three_prime_UTR |
| TACGAAGCAATGTTGAAGAA+GGG | 0.616487 | tig0023681:-44970 | None:intergenic |
| TTAAACAAGTTTCTAGAACA+GGG | 0.624594 | tig0023681:+44996 | Msa1410640:CDS |
| ATGAGCATGATTCTGACACT+GGG | 0.630580 | tig0023681:+45044 | Msa1410640:CDS |
| TAGGTCACGTTATCAGAAAG+CGG | 0.661044 | tig0023681:-44698 | None:intergenic |
| GAACAGAATAGCATGCAGAG+AGG | 0.743502 | tig0023681:+44791 | Msa1410640:CDS |
| AACGTGACCTATGAAAGTCG+CGG | 0.766478 | tig0023681:+44710 | Msa1410640:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATAATATGTTATTTGATA+CGG | - | tig0023681:44924-44943 | None:intergenic | 10.0% |
| !!! | AATTTTTTATATTCATGATA+GGG | - | tig0023681:45193-45212 | None:intergenic | 10.0% |
| !!! | TAATTTTTTATATTCATGAT+AGG | - | tig0023681:45194-45213 | None:intergenic | 10.0% |
| !!! | AAATAATATGTTATTTGATA+CGG | - | tig0023681:44924-44943 | None:intergenic | 10.0% |
| !!! | AATTTTTTATATTCATGATA+GGG | - | tig0023681:45193-45212 | None:intergenic | 10.0% |
| !!! | TAATTTTTTATATTCATGAT+AGG | - | tig0023681:45194-45213 | None:intergenic | 10.0% |
| !! | ATTGGAAATAAAATAGATAT+TGG | + | tig0023681:44875-44894 | Msa1410640:intron | 15.0% |
| !!! | AACAAAGAAAATATATTTTG+AGG | + | tig0023681:45097-45116 | Msa1410640:CDS | 15.0% |
| !! | ATGAATATAAAATTATCGAT+GGG | + | tig0023681:45158-45177 | Msa1410640:three_prime_UTR | 15.0% |
| !! | TGAATATAAAAAATTATCGA+TGG | + | tig0023681:45198-45217 | Msa1410640:three_prime_UTR | 15.0% |
| !! | GAATATAAAAAATTATCGAT+GGG | + | tig0023681:45199-45218 | Msa1410640:three_prime_UTR | 15.0% |
| !! | ATTGGAAATAAAATAGATAT+TGG | + | tig0023681:44875-44894 | Msa1410640:intron | 15.0% |
| !!! | AACAAAGAAAATATATTTTG+AGG | + | tig0023681:45097-45116 | Msa1410640:CDS | 15.0% |
| !! | ATGAATATAAAATTATCGAT+GGG | + | tig0023681:45158-45177 | Msa1410640:three_prime_UTR | 15.0% |
| !! | TGAATATAAAAAATTATCGA+TGG | + | tig0023681:45198-45217 | Msa1410640:three_prime_UTR | 15.0% |
| !! | GAATATAAAAAATTATCGAT+GGG | + | tig0023681:45199-45218 | Msa1410640:three_prime_UTR | 15.0% |
| !! | AGTAATAGGAATATAAAAGT+TGG | + | tig0023681:44416-44435 | Msa1410640:CDS | 20.0% |
| !! | ATTCCAAAAAAGAGATATTA+AGG | + | tig0023681:44457-44476 | Msa1410640:CDS | 20.0% |
| !!! | TATCCTTAATATCTCTTTTT+TGG | - | tig0023681:44463-44482 | None:intergenic | 20.0% |
| !!! | GAAACAAATTTTTTCTTTCA+TGG | + | tig0023681:44743-44762 | Msa1410640:CDS | 20.0% |
| !!! | CCAATAAAATCATTTTCATT+GGG | - | tig0023681:44860-44879 | None:intergenic | 20.0% |
| !!! | TCCAATAAAATCATTTTCAT+TGG | - | tig0023681:44861-44880 | None:intergenic | 20.0% |
| !! | TAACATATTATTTGTTTCAG+CGG | + | tig0023681:44931-44950 | Msa1410640:intron | 20.0% |
| !! | CATGAATATAAAATTATCGA+TGG | + | tig0023681:45157-45176 | Msa1410640:three_prime_UTR | 20.0% |
| !! | AGTAATAGGAATATAAAAGT+TGG | + | tig0023681:44416-44435 | Msa1410640:CDS | 20.0% |
| !! | ATTCCAAAAAAGAGATATTA+AGG | + | tig0023681:44457-44476 | Msa1410640:CDS | 20.0% |
| !!! | TATCCTTAATATCTCTTTTT+TGG | - | tig0023681:44463-44482 | None:intergenic | 20.0% |
| !!! | GAAACAAATTTTTTCTTTCA+TGG | + | tig0023681:44743-44762 | Msa1410640:CDS | 20.0% |
| !!! | CCAATAAAATCATTTTCATT+GGG | - | tig0023681:44860-44879 | None:intergenic | 20.0% |
| !!! | TCCAATAAAATCATTTTCAT+TGG | - | tig0023681:44861-44880 | None:intergenic | 20.0% |
| !! | TAACATATTATTTGTTTCAG+CGG | + | tig0023681:44931-44950 | Msa1410640:intron | 20.0% |
| !! | CATGAATATAAAATTATCGA+TGG | + | tig0023681:45157-45176 | Msa1410640:three_prime_UTR | 20.0% |
| !!! | GAAATAGTTTTGCTAGTAAT+AGG | + | tig0023681:44402-44421 | Msa1410640:CDS | 25.0% |
| !!! | ATCTCTTTTTTGGAATTTCA+TGG | - | tig0023681:44453-44472 | None:intergenic | 25.0% |
| ! | GATATTAAGGATAAGAGTTT+AGG | + | tig0023681:44470-44489 | Msa1410640:CDS | 25.0% |
| ! | GAAGTGAAATACATAATTGA+CGG | + | tig0023681:44509-44528 | Msa1410640:CDS | 25.0% |
| ! | AAGTGAAATACATAATTGAC+GGG | + | tig0023681:44510-44529 | Msa1410640:CDS | 25.0% |
| ! | TCTATCAAATACTCTAGTTA+AGG | - | tig0023681:44552-44571 | None:intergenic | 25.0% |
| ! | TATATCTGCTATCATTGAAA+TGG | - | tig0023681:44601-44620 | None:intergenic | 25.0% |
| !!! | ACAAATTTTTTCTTTCATGG+AGG | + | tig0023681:44746-44765 | Msa1410640:CDS | 25.0% |
| !! | CCCAATGAAAATGATTTTAT+TGG | + | tig0023681:44857-44876 | Msa1410640:intron | 25.0% |
| ! | AATAGATATTGGACTTTCAA+TGG | + | tig0023681:44886-44905 | Msa1410640:intron | 25.0% |
| !!! | ATTAAACAAGTTTCTAGAAC+AGG | + | tig0023681:44995-45014 | Msa1410640:CDS | 25.0% |
| !!! | TTAAACAAGTTTCTAGAACA+GGG | + | tig0023681:44996-45015 | Msa1410640:CDS | 25.0% |
| !! | TACATCGAATAAAGTACTTT+AGG | + | tig0023681:45224-45243 | Msa1410640:three_prime_UTR | 25.0% |
| ! | TGAATTAAGTTCATGTAAAG+TGG | - | tig0023681:45258-45277 | None:intergenic | 25.0% |
| !!! | GAAATAGTTTTGCTAGTAAT+AGG | + | tig0023681:44402-44421 | Msa1410640:CDS | 25.0% |
| !!! | ATCTCTTTTTTGGAATTTCA+TGG | - | tig0023681:44453-44472 | None:intergenic | 25.0% |
| ! | GATATTAAGGATAAGAGTTT+AGG | + | tig0023681:44470-44489 | Msa1410640:CDS | 25.0% |
| ! | GAAGTGAAATACATAATTGA+CGG | + | tig0023681:44509-44528 | Msa1410640:CDS | 25.0% |
| ! | AAGTGAAATACATAATTGAC+GGG | + | tig0023681:44510-44529 | Msa1410640:CDS | 25.0% |
| ! | TCTATCAAATACTCTAGTTA+AGG | - | tig0023681:44552-44571 | None:intergenic | 25.0% |
| ! | TATATCTGCTATCATTGAAA+TGG | - | tig0023681:44601-44620 | None:intergenic | 25.0% |
| !!! | ACAAATTTTTTCTTTCATGG+AGG | + | tig0023681:44746-44765 | Msa1410640:CDS | 25.0% |
| !! | CCCAATGAAAATGATTTTAT+TGG | + | tig0023681:44857-44876 | Msa1410640:intron | 25.0% |
| ! | AATAGATATTGGACTTTCAA+TGG | + | tig0023681:44886-44905 | Msa1410640:intron | 25.0% |
| !!! | ATTAAACAAGTTTCTAGAAC+AGG | + | tig0023681:44995-45014 | Msa1410640:CDS | 25.0% |
| !!! | TTAAACAAGTTTCTAGAACA+GGG | + | tig0023681:44996-45015 | Msa1410640:CDS | 25.0% |
| !! | TACATCGAATAAAGTACTTT+AGG | + | tig0023681:45224-45243 | Msa1410640:three_prime_UTR | 25.0% |
| ! | TGAATTAAGTTCATGTAAAG+TGG | - | tig0023681:45258-45277 | None:intergenic | 25.0% |
| ! | ACTAGAGTATTTGATAGAGA+TGG | + | tig0023681:44554-44573 | Msa1410640:CDS | 30.0% |
| !!! | AATTTTTTCTTTCATGGAGG+AGG | + | tig0023681:44749-44768 | Msa1410640:CDS | 30.0% |
| ATATTATTTGTTTCAGCGGA+CGG | + | tig0023681:44935-44954 | Msa1410640:intron | 30.0% | |
| ! | ACTAGAGTATTTGATAGAGA+TGG | + | tig0023681:44554-44573 | Msa1410640:CDS | 30.0% |
| !!! | AATTTTTTCTTTCATGGAGG+AGG | + | tig0023681:44749-44768 | Msa1410640:CDS | 30.0% |
| ATATTATTTGTTTCAGCGGA+CGG | + | tig0023681:44935-44954 | Msa1410640:intron | 30.0% | |
| GAATCCTTACAAAGTTACTG+TGG | + | tig0023681:44817-44836 | Msa1410640:intron | 35.0% | |
| TACGAAGCAATGTTGAAGAA+GGG | - | tig0023681:44973-44992 | None:intergenic | 35.0% | |
| ATACGAAGCAATGTTGAAGA+AGG | - | tig0023681:44974-44993 | None:intergenic | 35.0% | |
| !!! | AAGTTTCTAGAACAGGGTTT+TGG | + | tig0023681:45002-45021 | Msa1410640:CDS | 35.0% |
| GAATCCTTACAAAGTTACTG+TGG | + | tig0023681:44817-44836 | Msa1410640:intron | 35.0% | |
| TACGAAGCAATGTTGAAGAA+GGG | - | tig0023681:44973-44992 | None:intergenic | 35.0% | |
| ATACGAAGCAATGTTGAAGA+AGG | - | tig0023681:44974-44993 | None:intergenic | 35.0% | |
| !!! | AAGTTTCTAGAACAGGGTTT+TGG | + | tig0023681:45002-45021 | Msa1410640:CDS | 35.0% |
| TAGGTCACGTTATCAGAAAG+CGG | - | tig0023681:44701-44720 | None:intergenic | 40.0% | |
| AAAGAATCCGCGACTTTCAT+AGG | - | tig0023681:44720-44739 | None:intergenic | 40.0% | |
| TGAAAGTCGCGGATTCTTTA+AGG | + | tig0023681:44721-44740 | Msa1410640:CDS | 40.0% | |
| ! | TTTTCTTTCATGGAGGAGGA+AGG | + | tig0023681:44753-44772 | Msa1410640:CDS | 40.0% |
| TTTCTTTCATGGAGGAGGAA+GGG | + | tig0023681:44754-44773 | Msa1410640:CDS | 40.0% | |
| TGTGCCACAGTAACTTTGTA+AGG | - | tig0023681:44824-44843 | None:intergenic | 40.0% | |
| !!! | TTCTAGAACAGGGTTTTGGA+AGG | + | tig0023681:45006-45025 | Msa1410640:CDS | 40.0% |
| !!! | TCTAGAACAGGGTTTTGGAA+GGG | + | tig0023681:45007-45026 | Msa1410640:CDS | 40.0% |
| ATGAGCATGATTCTGACACT+GGG | + | tig0023681:45044-45063 | Msa1410640:CDS | 40.0% | |
| TAGGTCACGTTATCAGAAAG+CGG | - | tig0023681:44701-44720 | None:intergenic | 40.0% | |
| AAAGAATCCGCGACTTTCAT+AGG | - | tig0023681:44720-44739 | None:intergenic | 40.0% | |
| TGAAAGTCGCGGATTCTTTA+AGG | + | tig0023681:44721-44740 | Msa1410640:CDS | 40.0% | |
| ! | TTTTCTTTCATGGAGGAGGA+AGG | + | tig0023681:44753-44772 | Msa1410640:CDS | 40.0% |
| TTTCTTTCATGGAGGAGGAA+GGG | + | tig0023681:44754-44773 | Msa1410640:CDS | 40.0% | |
| TGTGCCACAGTAACTTTGTA+AGG | - | tig0023681:44824-44843 | None:intergenic | 40.0% | |
| !!! | TTCTAGAACAGGGTTTTGGA+AGG | + | tig0023681:45006-45025 | Msa1410640:CDS | 40.0% |
| !!! | TCTAGAACAGGGTTTTGGAA+GGG | + | tig0023681:45007-45026 | Msa1410640:CDS | 40.0% |
| ATGAGCATGATTCTGACACT+GGG | + | tig0023681:45044-45063 | Msa1410640:CDS | 40.0% | |
| GATAGAGATGGTAGCACTCA+AGG | + | tig0023681:44566-44585 | Msa1410640:CDS | 45.0% | |
| AACGTGACCTATGAAAGTCG+CGG | + | tig0023681:44710-44729 | Msa1410640:CDS | 45.0% | |
| TTCTTTCATGGAGGAGGAAG+GGG | + | tig0023681:44755-44774 | Msa1410640:CDS | 45.0% | |
| GAACAGAATAGCATGCAGAG+AGG | + | tig0023681:44791-44810 | Msa1410640:CDS | 45.0% | |
| !! | GTTGAAGAAGGGTGTTGAAC+AGG | - | tig0023681:44962-44981 | None:intergenic | 45.0% |
| CATGAGCATGATTCTGACAC+TGG | + | tig0023681:45043-45062 | Msa1410640:CDS | 45.0% | |
| GATAGAGATGGTAGCACTCA+AGG | + | tig0023681:44566-44585 | Msa1410640:CDS | 45.0% | |
| AACGTGACCTATGAAAGTCG+CGG | + | tig0023681:44710-44729 | Msa1410640:CDS | 45.0% | |
| TTCTTTCATGGAGGAGGAAG+GGG | + | tig0023681:44755-44774 | Msa1410640:CDS | 45.0% | |
| GAACAGAATAGCATGCAGAG+AGG | + | tig0023681:44791-44810 | Msa1410640:CDS | 45.0% | |
| !! | GTTGAAGAAGGGTGTTGAAC+AGG | - | tig0023681:44962-44981 | None:intergenic | 45.0% |
| CATGAGCATGATTCTGACAC+TGG | + | tig0023681:45043-45062 | Msa1410640:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0023681 | gene | 44398 | 45279 | 44398 | ID=Msa1410640;Name=Msa1410640 |
| tig0023681 | mRNA | 44398 | 45279 | 44398 | ID=Msa1410640-mRNA-1;Parent=Msa1410640;Name=Msa1410640-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|0.5|0.33|1|0|0|3|162|173 |
| tig0023681 | exon | 44398 | 44587 | 44398 | ID=Msa1410640-mRNA-1:exon:26665;Parent=Msa1410640-mRNA-1 |
| tig0023681 | exon | 44648 | 44812 | 44648 | ID=Msa1410640-mRNA-1:exon:26666;Parent=Msa1410640-mRNA-1 |
| tig0023681 | exon | 44951 | 45279 | 44951 | ID=Msa1410640-mRNA-1:exon:26667;Parent=Msa1410640-mRNA-1 |
| tig0023681 | CDS | 44398 | 44587 | 44398 | ID=Msa1410640-mRNA-1:cds;Parent=Msa1410640-mRNA-1 |
| tig0023681 | CDS | 44648 | 44812 | 44648 | ID=Msa1410640-mRNA-1:cds;Parent=Msa1410640-mRNA-1 |
| tig0023681 | CDS | 44951 | 45117 | 44951 | ID=Msa1410640-mRNA-1:cds;Parent=Msa1410640-mRNA-1 |
| tig0023681 | three_prime_UTR | 45118 | 45279 | 45118 | ID=Msa1410640-mRNA-1:three_prime_utr;Parent=Msa1410640-mRNA-1 |
| Gene Sequence |
| Protein sequence |
