AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1002590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1002590 MtrunA17_Chr5g0403851 98.830 171 2 0 1 171 1 171 5.63e-126 351
Msa1002590 MtrunA17_Chr3g0141491 67.416 89 26 2 48 133 6 94 1.88e-34 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1002590 AT4G36660.1 70.588 170 40 2 1 160 1 170 9.30e-79 232
Msa1002590 AT5G65650.1 69.186 172 46 2 1 165 1 172 3.19e-76 226
Msa1002590 AT1G19380.1 54.622 119 53 1 30 147 25 143 6.42e-37 125

Find 68 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAAGGAAGGTACAAATTT+TGG 0.077573 6_4:+88305299 Msa1002590:CDS
ATACATAATCATCTTGCTTA+TGG 0.256319 6_4:-88309000 None:intergenic
TTCCTTTGTCAGCTTGCTTA+AGG 0.283386 6_4:-88308849 None:intergenic
GGATCAGGTTGCCGAGGTTT+TGG 0.290545 6_4:+88305597 Msa1002590:CDS
ACTTGTGCATTTAGCATAGT+TGG 0.331228 6_4:-88309137 None:intergenic
AACGTGGATTCAGGTTTGTT+TGG 0.341765 6_4:+88305277 Msa1002590:CDS
ACCGTTTCTCTTCGTTGGTC+TGG 0.356252 6_4:+88305370 Msa1002590:CDS
AACTGACTATATGTTCTTGT+AGG 0.392162 6_4:+88309109 Msa1002590:three_prime_UTR
GACGGCAGCGATTAACAAAA+AGG 0.396473 6_4:+88305252 Msa1002590:CDS
ACATCGAGATCGTCGTGAAT+AGG 0.397094 6_4:-88305427 None:intergenic
TGTTGTTGTGGTAGATGAAC+GGG 0.406090 6_4:-88305083 None:intergenic
CTGGTACCGTTTCTCTTCGT+TGG 0.412184 6_4:+88305365 Msa1002590:CDS
TCAAGGGTCTGGCTTGTGCT+TGG 0.446945 6_4:+88308913 Msa1002590:three_prime_UTR
TTAATCGCTGCCGTCGTCGT+TGG 0.452555 6_4:-88305244 None:intergenic
ATCTATTATTATTGTTGTTG+TGG 0.459341 6_4:-88305095 None:intergenic
AGTATCGAGGTCACTGGATA+AGG 0.466687 6_4:-88305404 None:intergenic
AAGGAAAACGAACCGTGGAT+TGG 0.476537 6_4:-88305742 None:intergenic
ATTATTGTAGATTATTAGAT+CGG 0.476671 6_4:+88305198 Msa1002590:five_prime_UTR
GATTCAGGTTTGTTTGGAAA+AGG 0.481721 6_4:+88305283 Msa1002590:CDS
AGAAAGTTGTGAGGCATATG+TGG 0.488420 6_4:+88305552 Msa1002590:CDS
TAAAATCATTAACGCGGTGC+CGG 0.490162 6_4:+88304979 Msa1002590:five_prime_UTR
ACACCAACAGCCGTAGGATC+AGG 0.511562 6_4:+88305582 Msa1002590:CDS
TCTAGGAGCAGTCAAGGGTC+TGG 0.518590 6_4:+88308902 Msa1002590:three_prime_UTR
GACCACTCTAGGAGCAGTCA+AGG 0.524656 6_4:+88308896 Msa1002590:three_prime_UTR
TGTGTAGGAAATGGAAGAGA+GGG 0.530993 6_4:+88305529 Msa1002590:intron
CAGGTTTGTTTGGAAAAGGA+AGG 0.533040 6_4:+88305287 Msa1002590:CDS
GATTATTAGATCGGAAATGA+AGG 0.534187 6_4:+88305207 Msa1002590:exon
CAACCTGATCCTACGGCTGT+TGG 0.537984 6_4:-88305585 None:intergenic
GGAAGCATGAAAATCTGAGT+AGG 0.538740 6_4:-88308979 None:intergenic
CGAACCGTGGATTGGATAGG+AGG 0.542124 6_4:-88305734 None:intergenic
GCGTGCTTAAAGACCACTCT+AGG 0.544127 6_4:+88308885 Msa1002590:exon
AATCATTAACGCGGTGCCGG+TGG 0.544794 6_4:+88304982 Msa1002590:five_prime_UTR
CTTCATTTACACAATCAGAT+CGG 0.547440 6_4:+88305041 Msa1002590:intron
CGGAAATGAAGGATGACGAT+GGG 0.550371 6_4:+88305218 Msa1002590:exon
ACCAGACCAACGAAGAGAAA+CGG 0.550644 6_4:-88305371 None:intergenic
ACCCTTGACTGCTCCTAGAG+TGG 0.554244 6_4:-88308898 None:intergenic
ACCGTGGATTGGATAGGAGG+TGG 0.560306 6_4:-88305731 None:intergenic
AAACGAACCGTGGATTGGAT+AGG 0.568958 6_4:-88305737 None:intergenic
ACCTCGGCAACCTGATCCTA+CGG 0.573548 6_4:-88305592 None:intergenic
AATAGGAGTATCGAGGTCAC+TGG 0.574382 6_4:-88305410 None:intergenic
GAAGCATGAAAATCTGAGTA+GGG 0.578574 6_4:-88308978 None:intergenic
TTGTTGTTGTGGTAGATGAA+CGG 0.581539 6_4:-88305084 None:intergenic
AGCCTTAAGCAAGCTGACAA+AGG 0.595487 6_4:+88308847 Msa1002590:CDS
GAATTGACAAGTGATGTTCC+TGG 0.596743 6_4:+88305647 Msa1002590:CDS
CATCATATTTCAGAGAGCAC+AGG 0.597960 6_4:+88308813 Msa1002590:intron
GCTGTGATGCAAAATGAAGA+TGG 0.601135 6_4:-88304915 None:intergenic
GTGTGTAGGAAATGGAAGAG+AGG 0.601208 6_4:+88305528 Msa1002590:intron
AAGAGAGGGAGAAAGTTGTG+AGG 0.610515 6_4:+88305543 Msa1002590:CDS
AGAAACGGTACCAGTAAACA+TGG 0.615388 6_4:-88305356 None:intergenic
GCCGTAGGATCAGGTTGCCG+AGG 0.615991 6_4:+88305591 Msa1002590:CDS
AAAGCCTCTTGCCAAAACCT+CGG 0.619602 6_4:-88305608 None:intergenic
GAAAGTTGTGAGGCATATGT+GGG 0.620041 6_4:+88305553 Msa1002590:CDS
GTATAAATTGAATTTATGTG+TGG 0.628850 6_4:+88309211 Msa1002590:three_prime_UTR
TCCACCTCCTATCCAATCCA+CGG 0.641814 6_4:+88305730 Msa1002590:CDS
TCGGAAATGAAGGATGACGA+TGG 0.646801 6_4:+88305217 Msa1002590:exon
ACCACTCTAGGAGCAGTCAA+GGG 0.647727 6_4:+88308897 Msa1002590:three_prime_UTR
AAAAGATTGCGAAGAAACAG+TGG 0.656108 6_4:+88308946 Msa1002590:three_prime_UTR
CGATGGGCTTCCAACGACGA+CGG 0.657116 6_4:+88305234 Msa1002590:CDS
GATTGCGAAGAAACAGTGGT+GGG 0.658478 6_4:+88308950 Msa1002590:three_prime_UTR
AGATTGCGAAGAAACAGTGG+TGG 0.662809 6_4:+88308949 Msa1002590:three_prime_UTR
AATTTGTACGACAACAAACG+TGG 0.674259 6_4:-88309085 None:intergenic
ATAGCATCATCTCTAACACC+AGG 0.678366 6_4:-88305665 None:intergenic
ATGTGTACACCAACAGCCGT+AGG 0.683663 6_4:+88305576 Msa1002590:CDS
CGTCGTGAATAGGAGTATCG+AGG 0.701938 6_4:-88305417 None:intergenic
TGATAATAAAATCATTAACG+CGG 0.703473 6_4:+88304973 Msa1002590:five_prime_UTR
ATGTGAATGTGAGACACCAC+CGG 0.707275 6_4:-88304998 None:intergenic
AAAAGCGAGAAGCAAAATCG+CGG 0.727032 6_4:-88305329 None:intergenic
ACAAAGGAAAAGAAGCGATG+CGG 0.741207 6_4:+88308863 Msa1002590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTATTTTTAATAAA+GGG - chr6_4:88307949-88307968 None:intergenic 0.0%
!!! TTTATTTTTATTTTTAATAA+AGG - chr6_4:88307950-88307969 None:intergenic 0.0%
!!! ATTATATTTTAATTATTATT+TGG + chr6_4:88309167-88309186 Msa1002590:three_prime_UTR 0.0%
!! ATAAATCTTTATATGTTTAT+AGG - chr6_4:88306392-88306411 None:intergenic 10.0%
!! ATTATTGTAGATTATTAGAT+CGG + chr6_4:88305198-88305217 Msa1002590:five_prime_UTR 15.0%
!!! TTTACAATTTGTATGTTTTT+TGG + chr6_4:88306751-88306770 Msa1002590:intron 15.0%
!!! AGATGATTATGTATTTTAAT+AGG + chr6_4:88309009-88309028 Msa1002590:three_prime_UTR 15.0%
!!! TTGGTAAACAAATATTTTAT+TGG + chr6_4:88309186-88309205 Msa1002590:three_prime_UTR 15.0%
!!! TGGTAAACAAATATTTTATT+GGG + chr6_4:88309187-88309206 Msa1002590:three_prime_UTR 15.0%
!! TGATAATAAAATCATTAACG+CGG + chr6_4:88304973-88304992 Msa1002590:five_prime_UTR 20.0%
!!! ATCTATTATTATTGTTGTTG+TGG - chr6_4:88305098-88305117 None:intergenic 20.0%
!! AAAATGAGATTAAAGGAAAA+TGG - chr6_4:88305464-88305483 None:intergenic 20.0%
!! AAGAATCAAAATGAGATTAA+AGG - chr6_4:88305471-88305490 None:intergenic 20.0%
!!! ACTCATTGTTAATTTGTTAT+TGG + chr6_4:88305837-88305856 Msa1002590:intron 20.0%
!! ATAATATTGCACTAGAAATT+TGG + chr6_4:88305890-88305909 Msa1002590:intron 20.0%
!! GAACAAAAGATAAATTAAAG+TGG - chr6_4:88306123-88306142 None:intergenic 20.0%
!!! TTTTTAAATGTTGTTTCGTT+TGG + chr6_4:88306352-88306371 Msa1002590:intron 20.0%
!! TATATGTTTATAGGTGTTTA+TGG - chr6_4:88306383-88306402 None:intergenic 20.0%
!! TGTAAGTAAATAGACAAAAA+AGG - chr6_4:88307033-88307052 None:intergenic 20.0%
!! GATATTGTTAGTTAGATAAA+AGG + chr6_4:88307242-88307261 Msa1002590:intron 20.0%
!!! TTCATATTTGATAGTTTAAC+TGG + chr6_4:88307281-88307300 Msa1002590:intron 20.0%
!!! AAAAAATCAATTTTAGATGG+AGG - chr6_4:88307839-88307858 None:intergenic 20.0%
!!! CACAAAAAATCAATTTTAGA+TGG - chr6_4:88307842-88307861 None:intergenic 20.0%
!!! TGTTTATGTCAATGATTTTA+AGG + chr6_4:88307890-88307909 Msa1002590:intron 20.0%
!!! GTTTATGTCAATGATTTTAA+GGG + chr6_4:88307891-88307910 Msa1002590:intron 20.0%
!!! TGTTTAGTTTTGTTTTCTAA+GGG - chr6_4:88308190-88308209 None:intergenic 20.0%
!!! ATGTTTAGTTTTGTTTTCTA+AGG - chr6_4:88308191-88308210 None:intergenic 20.0%
!! TAAAATGAATGTTTGAATAG+AGG - chr6_4:88308250-88308269 None:intergenic 20.0%
!!! ATCATTTTGGAAACATTTTA+AGG + chr6_4:88308329-88308348 Msa1002590:intron 20.0%
!!! TCATTTTGGAAACATTTTAA+GGG + chr6_4:88308330-88308349 Msa1002590:intron 20.0%
!! GAAAGACTAATTAAAACAAA+AGG - chr6_4:88308443-88308462 None:intergenic 20.0%
!! TAATCTAAATTAACTGAGAA+TGG - chr6_4:88308475-88308494 None:intergenic 20.0%
!!! TTTTGTCAAAAAAGAAATGT+AGG + chr6_4:88308571-88308590 Msa1002590:intron 20.0%
!! TCATACTAGAGTATATATTA+TGG + chr6_4:88308774-88308793 Msa1002590:intron 20.0%
!!! TTATGTATTTTAATAGGTTG+TGG + chr6_4:88309015-88309034 Msa1002590:three_prime_UTR 20.0%
!! GTATAAATTGAATTTATGTG+TGG + chr6_4:88309211-88309230 Msa1002590:three_prime_UTR 20.0%
!!! AAAAGGAAGGTACAAATTTT+GGG + chr6_4:88305300-88305319 Msa1002590:CDS 25.0%
! AACATTAACTAACAAAACCA+TGG - chr6_4:88305775-88305794 None:intergenic 25.0%
!!! TTGTTAATTTGTTATTGGTC+TGG + chr6_4:88305842-88305861 Msa1002590:intron 25.0%
!!! TTTTTTGTCAAGTCTTTTTG+TGG - chr6_4:88306239-88306258 None:intergenic 25.0%
! CAGACTAATTTCAACTAATA+CGG - chr6_4:88306323-88306342 None:intergenic 25.0%
!!! GATTGGTTTTATTTTGAATG+TGG + chr6_4:88306494-88306513 Msa1002590:intron 25.0%
! TATGTTTGCAATTTGCAAAT+GGG + chr6_4:88306539-88306558 Msa1002590:intron 25.0%
!! CATTATTTAAAGCATTTCCT+GGG + chr6_4:88306591-88306610 Msa1002590:intron 25.0%
!!! TCTTTGTTGTTTGCATAATA+CGG + chr6_4:88306796-88306815 Msa1002590:intron 25.0%
! TGAAATCAGTTTACATTACA+AGG + chr6_4:88306830-88306849 Msa1002590:intron 25.0%
!! ATTCAACTTTAAAAGTGATG+TGG - chr6_4:88306973-88306992 None:intergenic 25.0%
!!! CATCACTTTTAAAGTTGAAT+TGG + chr6_4:88306973-88306992 Msa1002590:intron 25.0%
! AAAAGTTTATAAGTGTGTGT+AGG - chr6_4:88307009-88307028 None:intergenic 25.0%
! CAATTACATGGTAGAAATAA+TGG + chr6_4:88307107-88307126 Msa1002590:intron 25.0%
!! TCCAATTTGCATAAACTTTT+AGG + chr6_4:88307152-88307171 Msa1002590:intron 25.0%
! TCCTAAAAGTTTATGCAAAT+TGG - chr6_4:88307156-88307175 None:intergenic 25.0%
! TTAAGTGAACAAGAATTAGA+AGG + chr6_4:88307404-88307423 Msa1002590:intron 25.0%
!! CTTAAAAGTATAGTTAGAGA+GGG - chr6_4:88307469-88307488 None:intergenic 25.0%
!!! TAAAAGTTGAGCCTTTTTTA+GGG - chr6_4:88307773-88307792 None:intergenic 25.0%
! AAGTTTAGAAACTATTCTCA+AGG - chr6_4:88308083-88308102 None:intergenic 25.0%
! TTGAGAATAGTTTCTAAACT+TGG + chr6_4:88308082-88308101 Msa1002590:intron 25.0%
!! TAAACTTGGCATTTTTGTAT+TGG + chr6_4:88308096-88308115 Msa1002590:intron 25.0%
! AGTCTTCAAGAAAAATAAAC+TGG + chr6_4:88308213-88308232 Msa1002590:intron 25.0%
! GTCTTCAAGAAAAATAAACT+GGG + chr6_4:88308214-88308233 Msa1002590:intron 25.0%
! AAAGTAGAAAGAGATGTTAT+TGG + chr6_4:88308364-88308383 Msa1002590:intron 25.0%
! TTCTAAGCTCAATTATTTGA+AGG + chr6_4:88308640-88308659 Msa1002590:intron 25.0%
! TCTAAGCTCAATTATTTGAA+GGG + chr6_4:88308641-88308660 Msa1002590:intron 25.0%
! ATACATAATCATCTTGCTTA+TGG - chr6_4:88309003-88309022 None:intergenic 25.0%
CTTCATTTACACAATCAGAT+CGG + chr6_4:88305041-88305060 Msa1002590:intron 30.0%
GATTATTAGATCGGAAATGA+AGG + chr6_4:88305207-88305226 Msa1002590:exon 30.0%
GATTAACAAAAAGGAAAACG+TGG + chr6_4:88305261-88305280 Msa1002590:CDS 30.0%
!! GAAAAGGAAGGTACAAATTT+TGG + chr6_4:88305299-88305318 Msa1002590:CDS 30.0%
TTAACTAACAAAACCATGGA+TGG - chr6_4:88305771-88305790 None:intergenic 30.0%
TTACTTGAAATGATGAAGCT+CGG + chr6_4:88305813-88305832 Msa1002590:intron 30.0%
ATATTGCTGATCAAACTCAA+TGG + chr6_4:88305960-88305979 Msa1002590:intron 30.0%
AAGACTTAATTGAACGCTTA+TGG - chr6_4:88306191-88306210 None:intergenic 30.0%
GGAGAAATTTAACTCATGTT+TGG - chr6_4:88306218-88306237 None:intergenic 30.0%
CTATGTTTGCAATTTGCAAA+TGG + chr6_4:88306538-88306557 Msa1002590:intron 30.0%
ATGTTTGCAATTTGCAAATG+GGG + chr6_4:88306540-88306559 Msa1002590:intron 30.0%
! GCATTATTTAAAGCATTTCC+TGG + chr6_4:88306590-88306609 Msa1002590:intron 30.0%
AATTGTAAACCAACGAATCT+CGG - chr6_4:88306740-88306759 None:intergenic 30.0%
!!! GAGATCTTGTTTTCTTTGAA+AGG + chr6_4:88306930-88306949 Msa1002590:intron 30.0%
TGTCTATTTACTTACACTGT+TGG + chr6_4:88307037-88307056 Msa1002590:intron 30.0%
!! TGCATAAACTTTTAGGAAGA+TGG + chr6_4:88307159-88307178 Msa1002590:intron 30.0%
ATATGAAGAACATTGCAACA+GGG - chr6_4:88307268-88307287 None:intergenic 30.0%
AATATGAAGAACATTGCAAC+AGG - chr6_4:88307269-88307288 None:intergenic 30.0%
TCTTCTTTCCTTGAATCTTT+GGG - chr6_4:88307329-88307348 None:intergenic 30.0%
TTCTTCTTTCCTTGAATCTT+TGG - chr6_4:88307330-88307349 None:intergenic 30.0%
TAGCATCTTGGATCAATTAA+AGG - chr6_4:88307369-88307388 None:intergenic 30.0%
TTAATTGATCCAAGATGCTA+AGG + chr6_4:88307369-88307388 Msa1002590:intron 30.0%
GAATTTCCTGTACTAAATAG+AGG - chr6_4:88307433-88307452 None:intergenic 30.0%
! CCTTAAAAGTATAGTTAGAG+AGG - chr6_4:88307470-88307489 None:intergenic 30.0%
! CCTCTCTAACTATACTTTTA+AGG + chr6_4:88307467-88307486 Msa1002590:intron 30.0%
TAATTGGTGAAGATCCATTA+TGG + chr6_4:88307713-88307732 Msa1002590:intron 30.0%
! AGAAAATACAGGTTCTAGTT+TGG + chr6_4:88307746-88307765 Msa1002590:intron 30.0%
!!! CTAAAAGTTGAGCCTTTTTT+AGG - chr6_4:88307774-88307793 None:intergenic 30.0%
! ATCAATTTTAGATGGAGGTT+GGG - chr6_4:88307834-88307853 None:intergenic 30.0%
! AATCAATTTTAGATGGAGGT+TGG - chr6_4:88307835-88307854 None:intergenic 30.0%
GATGCAATCTAGGAATTTAA+AGG - chr6_4:88307982-88308001 None:intergenic 30.0%
CTTATGTGAAAAAAACGAGT+TGG + chr6_4:88308686-88308705 Msa1002590:intron 30.0%
! TTGAATTTAAGACTGAGCTA+AGG - chr6_4:88308748-88308767 None:intergenic 30.0%
TATGATGCAGTAAATACATC+AGG - chr6_4:88308800-88308819 None:intergenic 30.0%
AACTGACTATATGTTCTTGT+AGG + chr6_4:88309109-88309128 Msa1002590:three_prime_UTR 30.0%
CGATCTGATTGTGTAAATGA+AGG - chr6_4:88305043-88305062 None:intergenic 35.0%
! TTGTTGTTGTGGTAGATGAA+CGG - chr6_4:88305087-88305106 None:intergenic 35.0%
AAAAAGGAAAACGTGGATTC+AGG + chr6_4:88305268-88305287 Msa1002590:CDS 35.0%
! GATTCAGGTTTGTTTGGAAA+AGG + chr6_4:88305283-88305302 Msa1002590:CDS 35.0%
CAAGTAGTTAGTTATGTCAG+TGG - chr6_4:88306016-88306035 None:intergenic 35.0%
AAAACCAATCAACAAGACTC+AGG - chr6_4:88306484-88306503 None:intergenic 35.0%
AATTTGCAAATGGGGTTGTT+TGG + chr6_4:88306548-88306567 Msa1002590:intron 35.0%
! TTTTCCTACATAAGTCTCAC+AGG - chr6_4:88306676-88306695 None:intergenic 35.0%
ATTGTAAACCAACGAATCTC+GGG - chr6_4:88306739-88306758 None:intergenic 35.0%
ATAGTTTAACTGGAAGTTCC+TGG + chr6_4:88307291-88307310 Msa1002590:intron 35.0%
TACAATCACCCAAAGATTCA+AGG + chr6_4:88307318-88307337 Msa1002590:intron 35.0%
GATCAATTAAAGGACACTCT+GGG - chr6_4:88307359-88307378 None:intergenic 35.0%
CAATTATGACCTTAGCATCT+TGG - chr6_4:88307381-88307400 None:intergenic 35.0%
AGGTTTCCTCTATTTAGTAC+AGG + chr6_4:88307424-88307443 Msa1002590:intron 35.0%
CTTCGTCTTGGATCAATTAA+AGG - chr6_4:88307499-88307518 None:intergenic 35.0%
CAAACTTGTGACGAATTAGA+TGG + chr6_4:88307534-88307553 Msa1002590:intron 35.0%
GTCATGTCAAGTCATAAGTA+TGG + chr6_4:88307569-88307588 Msa1002590:intron 35.0%
!! CCACACTATTTCTGCATTAT+CGG - chr6_4:88307667-88307686 None:intergenic 35.0%
AATGCAGAAATAGTGTGGTT+AGG + chr6_4:88307669-88307688 Msa1002590:intron 35.0%
! TCTAGTTTGGTCCCTAAAAA+AGG + chr6_4:88307759-88307778 Msa1002590:intron 35.0%
AATGTTTGAATAGAGGACTC+AGG - chr6_4:88308243-88308262 None:intergenic 35.0%
TTTCCAAAATGATGACACAG+TGG - chr6_4:88308322-88308341 None:intergenic 35.0%
AAAGGAATGCAAATGTGAAG+AGG - chr6_4:88308425-88308444 None:intergenic 35.0%
AGATAATCACTTGACTCTCA+AGG + chr6_4:88308497-88308516 Msa1002590:intron 35.0%
GATAATCACTTGACTCTCAA+GGG + chr6_4:88308498-88308517 Msa1002590:intron 35.0%
AAAAGATTGCGAAGAAACAG+TGG + chr6_4:88308946-88308965 Msa1002590:three_prime_UTR 35.0%
GAAGCATGAAAATCTGAGTA+GGG - chr6_4:88308981-88309000 None:intergenic 35.0%
AATTTGTACGACAACAAACG+TGG - chr6_4:88309088-88309107 None:intergenic 35.0%
ACTTGTGCATTTAGCATAGT+TGG - chr6_4:88309140-88309159 None:intergenic 35.0%
GCTGTGATGCAAAATGAAGA+TGG - chr6_4:88304918-88304937 None:intergenic 40.0%
TAAAATCATTAACGCGGTGC+CGG + chr6_4:88304979-88304998 Msa1002590:five_prime_UTR 40.0%
TGTTGTTGTGGTAGATGAAC+GGG - chr6_4:88305086-88305105 None:intergenic 40.0%
! AACGTGGATTCAGGTTTGTT+TGG + chr6_4:88305277-88305296 Msa1002590:CDS 40.0%
! CAGGTTTGTTTGGAAAAGGA+AGG + chr6_4:88305287-88305306 Msa1002590:CDS 40.0%
AAAAGCGAGAAGCAAAATCG+CGG - chr6_4:88305332-88305351 None:intergenic 40.0%
!! CGATTTTGCTTCTCGCTTTT+TGG + chr6_4:88305332-88305351 Msa1002590:CDS 40.0%
!! GCTTTTTGGTCCATGTTTAC+TGG + chr6_4:88305346-88305365 Msa1002590:CDS 40.0%
AGAAACGGTACCAGTAAACA+TGG - chr6_4:88305359-88305378 None:intergenic 40.0%
GAATGTTTGTTTGCGTGTGT+AGG + chr6_4:88305514-88305533 Msa1002590:intron 40.0%
TTGTTTGCGTGTGTAGGAAA+TGG + chr6_4:88305520-88305539 Msa1002590:intron 40.0%
TGTGTAGGAAATGGAAGAGA+GGG + chr6_4:88305529-88305548 Msa1002590:intron 40.0%
AGAAAGTTGTGAGGCATATG+TGG + chr6_4:88305552-88305571 Msa1002590:CDS 40.0%
GAAAGTTGTGAGGCATATGT+GGG + chr6_4:88305553-88305572 Msa1002590:CDS 40.0%
GAATTGACAAGTGATGTTCC+TGG + chr6_4:88305647-88305666 Msa1002590:CDS 40.0%
ATAGCATCATCTCTAACACC+AGG - chr6_4:88305668-88305687 None:intergenic 40.0%
ACAAAACCATGGATGGAGAA+AGG - chr6_4:88305764-88305783 None:intergenic 40.0%
TCAAACTCAATGGTCCTGTT+TGG + chr6_4:88305970-88305989 Msa1002590:intron 40.0%
TCAATGGTCCTGTTTGGATT+GGG + chr6_4:88305976-88305995 Msa1002590:intron 40.0%
TCGTATAACCCAATCCAAAC+AGG - chr6_4:88305987-88306006 None:intergenic 40.0%
! AGACTCTTTGAGAGAGCTTA+TGG + chr6_4:88306038-88306057 Msa1002590:intron 40.0%
GTGATGTGGATGCATAAAGA+GGG - chr6_4:88306959-88306978 None:intergenic 40.0%
AGTGATGTGGATGCATAAAG+AGG - chr6_4:88306960-88306979 None:intergenic 40.0%
GTGTCATCACGAGTTAGTAA+AGG - chr6_4:88307083-88307102 None:intergenic 40.0%
GATGACACGACTCAATTACA+TGG + chr6_4:88307095-88307114 Msa1002590:intron 40.0%
GGATCAATTAAAGGACACTC+TGG - chr6_4:88307360-88307379 None:intergenic 40.0%
! ATGAACTGTTAGCTTCGTCT+TGG - chr6_4:88307511-88307530 None:intergenic 40.0%
CCGATAATGCAGAAATAGTG+TGG + chr6_4:88307664-88307683 Msa1002590:intron 40.0%
AAGATCCATTATGGTCACTG+AGG + chr6_4:88307722-88307741 Msa1002590:intron 40.0%
TTCTTCCTCAGTGACCATAA+TGG - chr6_4:88307730-88307749 None:intergenic 40.0%
GTCACTGAGGAAGAAAATAC+AGG + chr6_4:88307735-88307754 Msa1002590:intron 40.0%
GTCCTCGCAACAAATAAACT+AGG + chr6_4:88307793-88307812 Msa1002590:intron 40.0%
! GTCCTAGTTTATTTGTTGCG+AGG - chr6_4:88307798-88307817 None:intergenic 40.0%
TCAGGAAGAAGATGCAATCT+AGG - chr6_4:88307992-88308011 None:intergenic 40.0%
CTATTCTCAAGGACCAGAAT+AGG - chr6_4:88308072-88308091 None:intergenic 40.0%
TGCTCCTTGAGAAAAAAACC+TGG + chr6_4:88308151-88308170 Msa1002590:intron 40.0%
GCTCCTTGAGAAAAAAACCT+GGG + chr6_4:88308152-88308171 Msa1002590:intron 40.0%
!!! GTTCCCAGGTTTTTTTCTCA+AGG - chr6_4:88308158-88308177 None:intergenic 40.0%
AAAAAACCTGGGAACCAGTT+TGG + chr6_4:88308163-88308182 Msa1002590:intron 40.0%
!! TGTTTTCTAAGGGACCAAAC+TGG - chr6_4:88308180-88308199 None:intergenic 40.0%
! TGTCCACTGTGTCATCATTT+TGG + chr6_4:88308316-88308335 Msa1002590:intron 40.0%
GGAATTTAGCGAAAGTAGAG+AGG + chr6_4:88308519-88308538 Msa1002590:intron 40.0%
! CATCATATTTCAGAGAGCAC+AGG + chr6_4:88308813-88308832 Msa1002590:intron 40.0%
! TTCCTTTGTCAGCTTGCTTA+AGG - chr6_4:88308852-88308871 None:intergenic 40.0%
ACAAAGGAAAAGAAGCGATG+CGG + chr6_4:88308863-88308882 Msa1002590:CDS 40.0%
! GGAAGCATGAAAATCTGAGT+AGG - chr6_4:88308982-88309001 None:intergenic 40.0%
ATGTGAATGTGAGACACCAC+CGG - chr6_4:88305001-88305020 None:intergenic 45.0%
TCGGAAATGAAGGATGACGA+TGG + chr6_4:88305217-88305236 Msa1002590:exon 45.0%
CGGAAATGAAGGATGACGAT+GGG + chr6_4:88305218-88305237 Msa1002590:exon 45.0%
GACGGCAGCGATTAACAAAA+AGG + chr6_4:88305252-88305271 Msa1002590:CDS 45.0%
ACCAGACCAACGAAGAGAAA+CGG - chr6_4:88305374-88305393 None:intergenic 45.0%
! AGTATCGAGGTCACTGGATA+AGG - chr6_4:88305407-88305426 None:intergenic 45.0%
!! AATAGGAGTATCGAGGTCAC+TGG - chr6_4:88305413-88305432 None:intergenic 45.0%
ACATCGAGATCGTCGTGAAT+AGG - chr6_4:88305430-88305449 None:intergenic 45.0%
GTGTGTAGGAAATGGAAGAG+AGG + chr6_4:88305528-88305547 Msa1002590:intron 45.0%
AAGAGAGGGAGAAAGTTGTG+AGG + chr6_4:88305543-88305562 Msa1002590:CDS 45.0%
AAAGCCTCTTGCCAAAACCT+CGG - chr6_4:88305611-88305630 None:intergenic 45.0%
!! GTTTTGGCAAGAGGCTTTTG+AGG + chr6_4:88305613-88305632 Msa1002590:CDS 45.0%
!! AAACGAACCGTGGATTGGAT+AGG - chr6_4:88305740-88305759 None:intergenic 45.0%
AAGGAAAACGAACCGTGGAT+TGG - chr6_4:88305745-88305764 None:intergenic 45.0%
! CGTTTTCCTTTCTCCATCCA+TGG + chr6_4:88305755-88305774 Msa1002590:intron 45.0%
CTCAATGGTCCTGTTTGGAT+TGG + chr6_4:88305975-88305994 Msa1002590:intron 45.0%
CCAATCAACAAGACTCAGGT+TGG - chr6_4:88306480-88306499 None:intergenic 45.0%
!! CCAACCTGAGTCTTGTTGAT+TGG + chr6_4:88306477-88306496 Msa1002590:intron 45.0%
TGCAAATGGGGTTGTTTGGT+TGG + chr6_4:88306552-88306571 Msa1002590:intron 45.0%
GCAAATGGGGTTGTTTGGTT+GGG + chr6_4:88306553-88306572 Msa1002590:intron 45.0%
CAAATGGGGTTGTTTGGTTG+GGG + chr6_4:88306554-88306573 Msa1002590:intron 45.0%
TTGCATGAGACTTCTTACCC+AGG - chr6_4:88306611-88306630 None:intergenic 45.0%
TTCGCCTGTGAGACTTATGT+AGG + chr6_4:88306669-88306688 Msa1002590:intron 45.0%
GTTTAACTGGAAGTTCCTGG+TGG + chr6_4:88307294-88307313 Msa1002590:intron 45.0%
ATGAAGCAAAGCTGTCTGTC+TGG + chr6_4:88307629-88307648 Msa1002590:intron 45.0%
GCAGAAATAGTGTGGTTAGG+AGG + chr6_4:88307672-88307691 Msa1002590:intron 45.0%
TTTAGATGGAGGTTGGGAGA+GGG - chr6_4:88307828-88307847 None:intergenic 45.0%
! TTTTAGATGGAGGTTGGGAG+AGG - chr6_4:88307829-88307848 None:intergenic 45.0%
GTCAGTCTGTAGTCATTGTC+AGG - chr6_4:88307918-88307937 None:intergenic 45.0%
ATAACGTCCAGGACGAAGAA+AGG - chr6_4:88308019-88308038 None:intergenic 45.0%
TTCGTCCTGGACGTTATTGA+AGG + chr6_4:88308022-88308041 Msa1002590:intron 45.0%
AAGGTCCTTCAATAACGTCC+AGG - chr6_4:88308030-88308049 None:intergenic 45.0%
TGGACGTTATTGAAGGACCT+TGG + chr6_4:88308029-88308048 Msa1002590:intron 45.0%
ATTGAAGGACCTTGGAGAAC+TGG + chr6_4:88308037-88308056 Msa1002590:intron 45.0%
TCTTGAACACCAGTTCTCCA+AGG - chr6_4:88308049-88308068 None:intergenic 45.0%
CTGGTGTTCAAGACCTATTC+TGG + chr6_4:88308056-88308075 Msa1002590:intron 45.0%
AATGTGAAGAGGCTCAGATG+CGG - chr6_4:88308414-88308433 None:intergenic 45.0%
GGCTTCTTCGCATATGTTAC+TGG - chr6_4:88308727-88308746 None:intergenic 45.0%
AGCCTTAAGCAAGCTGACAA+AGG + chr6_4:88308847-88308866 Msa1002590:CDS 45.0%
AGATTGCGAAGAAACAGTGG+TGG + chr6_4:88308949-88308968 Msa1002590:three_prime_UTR 45.0%
GATTGCGAAGAAACAGTGGT+GGG + chr6_4:88308950-88308969 Msa1002590:three_prime_UTR 45.0%
!! TTTAAAAGTTAAATAATAAT+TGG + chr6_4:88307697-88307716 Msa1002590:intron 5.0%
CTGGTACCGTTTCTCTTCGT+TGG + chr6_4:88305365-88305384 Msa1002590:CDS 50.0%
ACCGTTTCTCTTCGTTGGTC+TGG + chr6_4:88305370-88305389 Msa1002590:CDS 50.0%
!! CGTCGTGAATAGGAGTATCG+AGG - chr6_4:88305420-88305439 None:intergenic 50.0%
ATGTGTACACCAACAGCCGT+AGG + chr6_4:88305576-88305595 Msa1002590:CDS 50.0%
TCCACCTCCTATCCAATCCA+CGG + chr6_4:88305730-88305749 Msa1002590:CDS 50.0%
GGAGAAAGGAAAACGAACCG+TGG - chr6_4:88305750-88305769 None:intergenic 50.0%
TCATGCTTCCCGAGATTCGT+TGG + chr6_4:88306728-88306747 Msa1002590:intron 50.0%
TTTGGGTGATTGTAGCCACC+AGG - chr6_4:88307312-88307331 None:intergenic 50.0%
!! AGGACGAAGAAAGGCGTTTC+AGG - chr6_4:88308010-88308029 None:intergenic 50.0%
TGAAACGCCTTTCTTCGTCC+TGG + chr6_4:88308009-88308028 Msa1002590:intron 50.0%
GCGTGCTTAAAGACCACTCT+AGG + chr6_4:88308885-88308904 Msa1002590:exon 50.0%
! ACCACTCTAGGAGCAGTCAA+GGG + chr6_4:88308897-88308916 Msa1002590:three_prime_UTR 50.0%
! AATCATTAACGCGGTGCCGG+TGG + chr6_4:88304982-88305001 Msa1002590:five_prime_UTR 55.0%
TTAATCGCTGCCGTCGTCGT+TGG - chr6_4:88305247-88305266 None:intergenic 55.0%
ACACCAACAGCCGTAGGATC+AGG + chr6_4:88305582-88305601 Msa1002590:CDS 55.0%
CAACCTGATCCTACGGCTGT+TGG - chr6_4:88305588-88305607 None:intergenic 55.0%
ACCTCGGCAACCTGATCCTA+CGG - chr6_4:88305595-88305614 None:intergenic 55.0%
!! GGATCAGGTTGCCGAGGTTT+TGG + chr6_4:88305597-88305616 Msa1002590:CDS 55.0%
!! GTTGCCGAGGTTTTGGCAAG+AGG + chr6_4:88305604-88305623 Msa1002590:CDS 55.0%
!! ACCGTGGATTGGATAGGAGG+TGG - chr6_4:88305734-88305753 None:intergenic 55.0%
!! CGAACCGTGGATTGGATAGG+AGG - chr6_4:88305737-88305756 None:intergenic 55.0%
GGCTGTGTCAGCAAACAGAG+AGG + chr6_4:88307128-88307147 Msa1002590:intron 55.0%
! AAGGGACCAAACTGGTTCCC+AGG - chr6_4:88308172-88308191 None:intergenic 55.0%
GGTCCTAGACCTAGTGTTGC+CGG + chr6_4:88308385-88308404 Msa1002590:intron 55.0%
GTCCTAGACCTAGTGTTGCC+GGG + chr6_4:88308386-88308405 Msa1002590:intron 55.0%
! GACCACTCTAGGAGCAGTCA+AGG + chr6_4:88308896-88308915 Msa1002590:three_prime_UTR 55.0%
ACCCTTGACTGCTCCTAGAG+TGG - chr6_4:88308901-88308920 None:intergenic 55.0%
TCTAGGAGCAGTCAAGGGTC+TGG + chr6_4:88308902-88308921 Msa1002590:three_prime_UTR 55.0%
! TCAAGGGTCTGGCTTGTGCT+TGG + chr6_4:88308913-88308932 Msa1002590:three_prime_UTR 55.0%
! CGATGGGCTTCCAACGACGA+CGG + chr6_4:88305234-88305253 Msa1002590:CDS 60.0%
CACCCGGCAACACTAGGTCT+AGG - chr6_4:88308391-88308410 None:intergenic 60.0%
! GCCGTAGGATCAGGTTGCCG+AGG + chr6_4:88305591-88305610 Msa1002590:CDS 65.0%
ATGCGGCACCCGGCAACACT+AGG - chr6_4:88308397-88308416 None:intergenic 65.0%
AGAGGCTCAGATGCGGCACC+CGG - chr6_4:88308407-88308426 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 88304914 88309263 88304914 ID=Msa1002590;Name=Msa1002590
chr6_4 mRNA 88304914 88309263 88304914 ID=Msa1002590-mRNA-1;Parent=Msa1002590;Name=Msa1002590-mRNA-1;_AED=0.02;_eAED=0.02;_QI=267|1|1|1|0.5|0.4|5|369|171
chr6_4 exon 88304914 88304944 88304914 ID=Msa1002590-mRNA-1:exon:17218;Parent=Msa1002590-mRNA-1
chr6_4 exon 88304967 88305030 88304967 ID=Msa1002590-mRNA-1:exon:17219;Parent=Msa1002590-mRNA-1
chr6_4 exon 88305051 88305453 88305051 ID=Msa1002590-mRNA-1:exon:17220;Parent=Msa1002590-mRNA-1
chr6_4 exon 88305536 88305751 88305536 ID=Msa1002590-mRNA-1:exon:17221;Parent=Msa1002590-mRNA-1
chr6_4 exon 88308826 88309263 88308826 ID=Msa1002590-mRNA-1:exon:17222;Parent=Msa1002590-mRNA-1
chr6_4 five_prime_UTR 88304914 88304944 88304914 ID=Msa1002590-mRNA-1:five_prime_utr;Parent=Msa1002590-mRNA-1
chr6_4 five_prime_UTR 88304967 88305030 88304967 ID=Msa1002590-mRNA-1:five_prime_utr;Parent=Msa1002590-mRNA-1
chr6_4 five_prime_UTR 88305051 88305222 88305051 ID=Msa1002590-mRNA-1:five_prime_utr;Parent=Msa1002590-mRNA-1
chr6_4 CDS 88305223 88305453 88305223 ID=Msa1002590-mRNA-1:cds;Parent=Msa1002590-mRNA-1
chr6_4 CDS 88305536 88305751 88305536 ID=Msa1002590-mRNA-1:cds;Parent=Msa1002590-mRNA-1
chr6_4 CDS 88308826 88308894 88308826 ID=Msa1002590-mRNA-1:cds;Parent=Msa1002590-mRNA-1
chr6_4 three_prime_UTR 88308895 88309263 88308895 ID=Msa1002590-mRNA-1:three_prime_utr;Parent=Msa1002590-mRNA-1
Gene Sequence

>Msa1002590

ATGAAGGATGACGATGGGCTTCCAACGACGACGGCAGCGATTAACAAAAAGGAAAACGTGGATTCAGGTTTGTTTGGAAAAGGAAGGTACAAATTTTGGGCATTAGCCGCGATTTTGCTTCTCGCTTTTTGGTCCATGTTTACTGGTACCGTTTCTCTTCGTTGGTCTGGTAATCTTAATACCTTATCCAGTGACCTCGATACTCCTATTCACGACGATCTCGATGTTCTCGAAATGGAAGAGAGGGAGAAAGTTGTGAGGCATATGTGGGATGTGTACACCAACAGCCGTAGGATCAGGTTGCCGAGGTTTTGGCAAGAGGCTTTTGAGGCTGCTTATGAAGAATTGACAAGTGATGTTCCTGGTGTTAGAGATGATGCTATTACTGAGATTGCTAAGATGTCTGTGCGTTCTGTTAATTATGATCCACCTCCTATCCAATCCACGAGAGCACAGGAATTTAGTAAAAGCCTTAAGCAAGCTGACAAAGGAAAAGAAGCGATGCGGCGTGCTTAA
Protein sequence

>Msa1002590

MKDDDGLPTTTAAINKKENVDSGLFGKGRYKFWALAAILLLAFWSMFTGTVSLRWSGNLNTLSSDLDTPIHDDLDVLEMEEREKVVRHMWDVYTNSRRIRLPRFWQEAFEAAYEELTSDVPGVRDDAITEIAKMSVRSVNYDPPPIQSTRAQEFSKSLKQADKGKEAMRRA*