AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1003680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1003680 MtrunA17_Chr4g0001531 100.000 37 0 0 66 102 21 57 1.24e-23 85.5
Msa1003680 MtrunA17_Chr7g0214441 100.000 37 0 0 66 102 21 57 1.24e-23 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1003680 AT3G05870.4 100.000 36 0 0 66 101 54 89 1.75e-22 84.0
Msa1003680 AT3G05870.3 100.000 36 0 0 66 101 54 89 1.75e-22 84.0
Msa1003680 AT3G05870.5 100.000 36 0 0 66 101 54 89 1.75e-22 84.0
Msa1003680 AT3G05870.1 100.000 36 0 0 66 101 51 86 1.99e-22 83.6
Msa1003680 AT3G05870.2 100.000 36 0 0 66 101 51 86 1.99e-22 83.6
Msa1003680 AT3G05870.6 100.000 36 0 0 66 101 51 86 1.99e-22 83.6
Msa1003680 AT3G05870.7 100.000 36 0 0 66 101 42 77 2.60e-22 83.2

Find 43 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCTGTTGCTTCTTGGACT+TGG 0.254508 7_1:-751810 Msa1003680:CDS
TGTAATTTGATCATTTCTAT+TGG 0.277487 7_1:-749950 Msa1003680:three_prime_UTR
TGAAATTAGAGTTTCTAAAT+TGG 0.278981 7_1:-749870 Msa1003680:three_prime_UTR
GATGGCATGCTGTTGCTTCT+TGG 0.281016 7_1:-751816 Msa1003680:CDS
CGTCGGGAATGGCAATTTAA+AGG 0.290239 7_1:-750066 Msa1003680:CDS
CTCAGTAAGTTACATTATCT+TGG 0.303066 7_1:-751700 Msa1003680:CDS
TGCTGTTGCTTCTTGGACTT+GGG 0.306486 7_1:-751809 Msa1003680:CDS
ATTTACATTGTATCTTGAAA+TGG 0.311304 7_1:-750128 Msa1003680:CDS
CTGATGATGAAGTTAGGGTT+TGG 0.382760 7_1:+752148 None:intergenic
TACGTTTGAATTTGCGGGAC+TGG 0.390846 7_1:-752245 None:intergenic
TGTCCTGATTGTAAACTTCC+TGG 0.394301 7_1:-751736 Msa1003680:CDS
ATACATACGTTTGAATTTGC+GGG 0.399310 7_1:-752250 None:intergenic
GAAGAAGATTTGAATTGAAA+AGG 0.408369 7_1:+752174 None:intergenic
TTTACATTGTATCTTGAAAT+GGG 0.412787 7_1:-750127 Msa1003680:CDS
TCAGTAAGTTACATTATCTT+GGG 0.412800 7_1:-751699 Msa1003680:CDS
TAATATCTCCATTGTTTCCA+TGG 0.415568 7_1:-749896 Msa1003680:three_prime_UTR
TATACATACGTTTGAATTTG+CGG 0.415740 7_1:-752251 None:intergenic
TTCTTGGACTTGGGATGCAC+AGG 0.429937 7_1:-751800 Msa1003680:CDS
ATCTGTAGGATGGCCTTTGA+TGG 0.438695 7_1:-751763 Msa1003680:CDS
GCCATTCCCGACGACACATT+GGG 0.449214 7_1:+750076 None:intergenic
GTCCTGATTGTAAACTTCCT+GGG 0.462403 7_1:-751735 Msa1003680:CDS
AAACTCTAATTTCAATCCCA+TGG 0.474150 7_1:+749879 None:intergenic
TGCCATTCCCGACGACACAT+TGG 0.494237 7_1:+750075 None:intergenic
GCCCAATGTGTCGTCGGGAA+TGG 0.498823 7_1:-750077 Msa1003680:CDS
AATATCTCCATTGTTTCCAT+GGG 0.512610 7_1:-749895 Msa1003680:three_prime_UTR
CACATTGCCCAATGTGTCGT+CGG 0.513658 7_1:-750083 Msa1003680:CDS
ACTGGACAAGACAAAGGAAA+AGG 0.516733 7_1:-752227 Msa1003680:five_prime_UTR
TCCCCAGGAAGTTTACAATC+AGG 0.518100 7_1:+751733 None:intergenic
CATTGGGCAATGTGCTTGTG+AGG 0.523053 7_1:+750092 None:intergenic
TTTCAATCCCATGGAAACAA+TGG 0.523838 7_1:+749888 None:intergenic
GGATGTATTGCTTATGAATG+AGG 0.540581 7_1:-749929 Msa1003680:three_prime_UTR
ACATTGCCCAATGTGTCGTC+GGG 0.540781 7_1:-750082 Msa1003680:CDS
GATTTGAATTGAAAAGGAGA+AGG 0.548938 7_1:+752180 None:intergenic
TCCTGATTGTAAACTTCCTG+GGG 0.574605 7_1:-751734 Msa1003680:CDS
CTGGACAAGACAAAGGAAAA+GGG 0.575458 7_1:-752226 Msa1003680:five_prime_UTR
ATGAAACATGTGGGATCTGT+AGG 0.577911 7_1:-751777 Msa1003680:CDS
CTGAGTGGACAGTCATCCCC+AGG 0.578584 7_1:+751718 None:intergenic
ATCAGGACAGCATCCATCAA+AGG 0.590910 7_1:+751750 None:intergenic
TGCGGGACTGGACAAGACAA+AGG 0.628957 7_1:-752233 Msa1003680:five_prime_UTR
AACATGTGGGATCTGTAGGA+TGG 0.695036 7_1:-751773 Msa1003680:CDS
AGATAATGTAACTTACTGAG+TGG 0.697836 7_1:+751703 None:intergenic
GATGCACAGGATGAAACATG+TGG 0.715539 7_1:-751787 Msa1003680:CDS
ATGCACAGGATGAAACATGT+GGG 0.727822 7_1:-751786 Msa1003680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATTCAATAAAATAAACAA+TGG + chr7_1:750743-750762 None:intergenic 15.0%
!! TTATCTAAATGTTAAAACAA+AGG + chr7_1:750769-750788 None:intergenic 15.0%
!! AATATATTATTTGATATGTC+AGG + chr7_1:751830-751849 None:intergenic 15.0%
!! TCATTCAATAAAATAAACAA+TGG + chr7_1:750743-750762 None:intergenic 15.0%
!! TTATCTAAATGTTAAAACAA+AGG + chr7_1:750769-750788 None:intergenic 15.0%
!! AATATATTATTTGATATGTC+AGG + chr7_1:751830-751849 None:intergenic 15.0%
!! ACAACTAATGAATACATATT+AGG + chr7_1:750174-750193 None:intergenic 20.0%
!! AAAAATAGTAAACTCTCATT+TGG - chr7_1:750627-750646 Msa1003680:intron 20.0%
!! AGAAGAATCAACATAAATTT+CGG + chr7_1:751133-751152 None:intergenic 20.0%
!! TACATTCAGTAAAATTAGAA+AGG + chr7_1:751482-751501 None:intergenic 20.0%
!! ATTTACATTGTATCTTGAAA+TGG - chr7_1:751926-751945 Msa1003680:intron 20.0%
!! TTTACATTGTATCTTGAAAT+GGG - chr7_1:751927-751946 Msa1003680:intron 20.0%
!!! CAATGTAATTTTGTTTTTCA+TGG - chr7_1:752027-752046 Msa1003680:intron 20.0%
!!! AAGTAACTAAGATCTTTTTA+AGG - chr7_1:752077-752096 Msa1003680:intron 20.0%
!!! AGTAACTAAGATCTTTTTAA+GGG - chr7_1:752078-752097 Msa1003680:intron 20.0%
!! TGTAATTTGATCATTTCTAT+TGG - chr7_1:752104-752123 Msa1003680:intron 20.0%
!! TGAAATTAGAGTTTCTAAAT+TGG - chr7_1:752184-752203 Msa1003680:five_prime_UTR 20.0%
!!! ATATATTTTTTCATCCTTTG+TGG + chr7_1:752226-752245 None:intergenic 20.0%
!! ACAACTAATGAATACATATT+AGG + chr7_1:750174-750193 None:intergenic 20.0%
!! AAAAATAGTAAACTCTCATT+TGG - chr7_1:750627-750646 Msa1003680:intron 20.0%
!! AGAAGAATCAACATAAATTT+CGG + chr7_1:751133-751152 None:intergenic 20.0%
!! TACATTCAGTAAAATTAGAA+AGG + chr7_1:751482-751501 None:intergenic 20.0%
!! ATTTACATTGTATCTTGAAA+TGG - chr7_1:751926-751945 Msa1003680:intron 20.0%
!! TTTACATTGTATCTTGAAAT+GGG - chr7_1:751927-751946 Msa1003680:intron 20.0%
!!! CAATGTAATTTTGTTTTTCA+TGG - chr7_1:752027-752046 Msa1003680:intron 20.0%
!!! AAGTAACTAAGATCTTTTTA+AGG - chr7_1:752077-752096 Msa1003680:intron 20.0%
!!! AGTAACTAAGATCTTTTTAA+GGG - chr7_1:752078-752097 Msa1003680:intron 20.0%
!! TGTAATTTGATCATTTCTAT+TGG - chr7_1:752104-752123 Msa1003680:intron 20.0%
!! TGAAATTAGAGTTTCTAAAT+TGG - chr7_1:752184-752203 Msa1003680:five_prime_UTR 20.0%
!!! ATATATTTTTTCATCCTTTG+TGG + chr7_1:752226-752245 None:intergenic 20.0%
! GAAGAAGATTTGAATTGAAA+AGG + chr7_1:749883-749902 None:intergenic 25.0%
!!! ATTTTCTGATGATGAAGTTA+GGG + chr7_1:749914-749933 None:intergenic 25.0%
! TTCTCTAACTAACTTTATAC+AGG - chr7_1:750076-750095 Msa1003680:CDS 25.0%
! TCAGTAAGTTACATTATCTT+GGG - chr7_1:750355-750374 Msa1003680:intron 25.0%
! TATTTGCATACAATTGTCTT+TGG - chr7_1:750398-750417 Msa1003680:intron 25.0%
! GTCATTGAAATATGTTGAAA+TGG - chr7_1:750552-750571 Msa1003680:intron 25.0%
! AATGGAATTTAACAACACTT+CGG + chr7_1:750725-750744 None:intergenic 25.0%
! TCATGGTAACATATATATCA+CGG + chr7_1:750828-750847 None:intergenic 25.0%
! ACTAATTTCATCAATATTCC+AGG - chr7_1:750981-751000 Msa1003680:intron 25.0%
! AACATTCTTCTTAACAGAAT+AGG - chr7_1:751084-751103 Msa1003680:intron 25.0%
! AATGGATGAGATACTTTAAA+GGG + chr7_1:751183-751202 None:intergenic 25.0%
! AAATGGATGAGATACTTTAA+AGG + chr7_1:751184-751203 None:intergenic 25.0%
!!! TAAAGTATCTCATCCATTTT+TGG - chr7_1:751185-751204 Msa1003680:intron 25.0%
! TTTACCACGTCATTAATATA+TGG - chr7_1:751265-751284 Msa1003680:intron 25.0%
! TTACCACGTCATTAATATAT+GGG - chr7_1:751266-751285 Msa1003680:intron 25.0%
! ATACCTATAAACAACATTTG+TGG - chr7_1:751513-751532 Msa1003680:intron 25.0%
!! AAACCACAAATGTTGTTTAT+AGG + chr7_1:751519-751538 None:intergenic 25.0%
!! TTTATAAAGCTTTATGCTCA+AGG - chr7_1:751610-751629 Msa1003680:intron 25.0%
!!! CATGATTGAGAATTTTTCAA+GGG - chr7_1:751651-751670 Msa1003680:intron 25.0%
!!! TTTGTTTTTCATGGAAAACT+AGG - chr7_1:752036-752055 Msa1003680:intron 25.0%
!!! TTGTTTTTCATGGAAAACTA+GGG - chr7_1:752037-752056 Msa1003680:intron 25.0%
! GAAGAAGATTTGAATTGAAA+AGG + chr7_1:749883-749902 None:intergenic 25.0%
!!! ATTTTCTGATGATGAAGTTA+GGG + chr7_1:749914-749933 None:intergenic 25.0%
! TTCTCTAACTAACTTTATAC+AGG - chr7_1:750076-750095 Msa1003680:CDS 25.0%
! TCAGTAAGTTACATTATCTT+GGG - chr7_1:750355-750374 Msa1003680:intron 25.0%
! TATTTGCATACAATTGTCTT+TGG - chr7_1:750398-750417 Msa1003680:intron 25.0%
! GTCATTGAAATATGTTGAAA+TGG - chr7_1:750552-750571 Msa1003680:intron 25.0%
! AATGGAATTTAACAACACTT+CGG + chr7_1:750725-750744 None:intergenic 25.0%
! TCATGGTAACATATATATCA+CGG + chr7_1:750828-750847 None:intergenic 25.0%
! ACTAATTTCATCAATATTCC+AGG - chr7_1:750981-751000 Msa1003680:intron 25.0%
! AACATTCTTCTTAACAGAAT+AGG - chr7_1:751084-751103 Msa1003680:intron 25.0%
! AATGGATGAGATACTTTAAA+GGG + chr7_1:751183-751202 None:intergenic 25.0%
! AAATGGATGAGATACTTTAA+AGG + chr7_1:751184-751203 None:intergenic 25.0%
!!! TAAAGTATCTCATCCATTTT+TGG - chr7_1:751185-751204 Msa1003680:intron 25.0%
! TTTACCACGTCATTAATATA+TGG - chr7_1:751265-751284 Msa1003680:intron 25.0%
! TTACCACGTCATTAATATAT+GGG - chr7_1:751266-751285 Msa1003680:intron 25.0%
! ATACCTATAAACAACATTTG+TGG - chr7_1:751513-751532 Msa1003680:intron 25.0%
!! AAACCACAAATGTTGTTTAT+AGG + chr7_1:751519-751538 None:intergenic 25.0%
!! TTTATAAAGCTTTATGCTCA+AGG - chr7_1:751610-751629 Msa1003680:intron 25.0%
!!! CATGATTGAGAATTTTTCAA+GGG - chr7_1:751651-751670 Msa1003680:intron 25.0%
!!! TTTGTTTTTCATGGAAAACT+AGG - chr7_1:752036-752055 Msa1003680:intron 25.0%
!!! TTGTTTTTCATGGAAAACTA+GGG - chr7_1:752037-752056 Msa1003680:intron 25.0%
GATTTGAATTGAAAAGGAGA+AGG + chr7_1:749877-749896 None:intergenic 30.0%
!! CATTTTCTGATGATGAAGTT+AGG + chr7_1:749915-749934 None:intergenic 30.0%
GGTTGAATGCAAAAATGAAA+AGG + chr7_1:749958-749977 None:intergenic 30.0%
!! TGACTTAGTTTTCTCTCTAT+TGG - chr7_1:750011-750030 Msa1003680:three_prime_UTR 30.0%
CTAACTTTATACAGGTTGTT+TGG - chr7_1:750084-750103 Msa1003680:CDS 30.0%
!! CATATTAGGTTGATTTCACT+AGG + chr7_1:750160-750179 None:intergenic 30.0%
CTTATCTCATCAAATGTAGA+TGG - chr7_1:750220-750239 Msa1003680:intron 30.0%
AGATAATGTAACTTACTGAG+TGG + chr7_1:750354-750373 None:intergenic 30.0%
CTCAGTAAGTTACATTATCT+TGG - chr7_1:750354-750373 Msa1003680:intron 30.0%
ATTTCACAATTCAGCACAAT+AGG + chr7_1:750532-750551 None:intergenic 30.0%
! CAAAATAGACTTTTATGACG+TGG - chr7_1:750604-750623 Msa1003680:intron 30.0%
TAGTAAACTCTCATTTGGAT+TGG - chr7_1:750632-750651 Msa1003680:intron 30.0%
ATGGAATTTAACAACACTTC+GGG + chr7_1:750724-750743 None:intergenic 30.0%
AACATATATATCACGGTAGT+TGG + chr7_1:750821-750840 None:intergenic 30.0%
ACAATTGCATGATAGTTTCA+TGG + chr7_1:750845-750864 None:intergenic 30.0%
AAATAGATGCTGTAAAAAGC+AGG - chr7_1:750896-750915 Msa1003680:intron 30.0%
AATAGATGCTGTAAAAAGCA+GGG - chr7_1:750897-750916 Msa1003680:intron 30.0%
! GTACTTATTGGTTATGTTGT+AGG + chr7_1:751041-751060 None:intergenic 30.0%
GAACAAAATTAGGCCAAAAA+TGG + chr7_1:751201-751220 None:intergenic 30.0%
AAACCCATATATTAATGACG+TGG + chr7_1:751272-751291 None:intergenic 30.0%
!!! GATTTTTAGTTGAAACCAGT+TGG + chr7_1:751335-751354 None:intergenic 30.0%
! AATGTTGTTTATAGGTATCC+AGG + chr7_1:751511-751530 None:intergenic 30.0%
! GCATGATTGAGAATTTTTCA+AGG - chr7_1:751650-751669 Msa1003680:intron 30.0%
TAATATCTCCATTGTTTCCA+TGG - chr7_1:752158-752177 Msa1003680:five_prime_UTR 30.0%
AATATCTCCATTGTTTCCAT+GGG - chr7_1:752159-752178 Msa1003680:five_prime_UTR 30.0%
AAACTCTAATTTCAATCCCA+TGG + chr7_1:752178-752197 None:intergenic 30.0%
GATTTGAATTGAAAAGGAGA+AGG + chr7_1:749877-749896 None:intergenic 30.0%
!! CATTTTCTGATGATGAAGTT+AGG + chr7_1:749915-749934 None:intergenic 30.0%
GGTTGAATGCAAAAATGAAA+AGG + chr7_1:749958-749977 None:intergenic 30.0%
!! TGACTTAGTTTTCTCTCTAT+TGG - chr7_1:750011-750030 Msa1003680:three_prime_UTR 30.0%
CTAACTTTATACAGGTTGTT+TGG - chr7_1:750084-750103 Msa1003680:CDS 30.0%
!! CATATTAGGTTGATTTCACT+AGG + chr7_1:750160-750179 None:intergenic 30.0%
CTTATCTCATCAAATGTAGA+TGG - chr7_1:750220-750239 Msa1003680:intron 30.0%
AGATAATGTAACTTACTGAG+TGG + chr7_1:750354-750373 None:intergenic 30.0%
CTCAGTAAGTTACATTATCT+TGG - chr7_1:750354-750373 Msa1003680:intron 30.0%
ATTTCACAATTCAGCACAAT+AGG + chr7_1:750532-750551 None:intergenic 30.0%
! CAAAATAGACTTTTATGACG+TGG - chr7_1:750604-750623 Msa1003680:intron 30.0%
TAGTAAACTCTCATTTGGAT+TGG - chr7_1:750632-750651 Msa1003680:intron 30.0%
ATGGAATTTAACAACACTTC+GGG + chr7_1:750724-750743 None:intergenic 30.0%
AACATATATATCACGGTAGT+TGG + chr7_1:750821-750840 None:intergenic 30.0%
ACAATTGCATGATAGTTTCA+TGG + chr7_1:750845-750864 None:intergenic 30.0%
AAATAGATGCTGTAAAAAGC+AGG - chr7_1:750896-750915 Msa1003680:intron 30.0%
AATAGATGCTGTAAAAAGCA+GGG - chr7_1:750897-750916 Msa1003680:intron 30.0%
! GTACTTATTGGTTATGTTGT+AGG + chr7_1:751041-751060 None:intergenic 30.0%
GAACAAAATTAGGCCAAAAA+TGG + chr7_1:751201-751220 None:intergenic 30.0%
AAACCCATATATTAATGACG+TGG + chr7_1:751272-751291 None:intergenic 30.0%
!!! GATTTTTAGTTGAAACCAGT+TGG + chr7_1:751335-751354 None:intergenic 30.0%
! AATGTTGTTTATAGGTATCC+AGG + chr7_1:751511-751530 None:intergenic 30.0%
! GCATGATTGAGAATTTTTCA+AGG - chr7_1:751650-751669 Msa1003680:intron 30.0%
TAATATCTCCATTGTTTCCA+TGG - chr7_1:752158-752177 Msa1003680:five_prime_UTR 30.0%
AATATCTCCATTGTTTCCAT+GGG - chr7_1:752159-752178 Msa1003680:five_prime_UTR 30.0%
AAACTCTAATTTCAATCCCA+TGG + chr7_1:752178-752197 None:intergenic 30.0%
! TTTGAAGCAAAAGCACAAAC+AGG + chr7_1:749979-749998 None:intergenic 35.0%
ATTCAGCACAATAGGAAAGT+GGG + chr7_1:750524-750543 None:intergenic 35.0%
AATTCAGCACAATAGGAAAG+TGG + chr7_1:750525-750544 None:intergenic 35.0%
ATAGATGCTGTAAAAAGCAG+GGG - chr7_1:750898-750917 Msa1003680:intron 35.0%
CCACAAGATTGTGTACTTAT+TGG + chr7_1:751053-751072 None:intergenic 35.0%
CCAATAAGTACACAATCTTG+TGG - chr7_1:751050-751069 Msa1003680:intron 35.0%
!! CTTCTCTAAGATGACTATGT+TGG - chr7_1:751148-751167 Msa1003680:intron 35.0%
GAGTCAAGAGGAACAAAATT+AGG + chr7_1:751211-751230 None:intergenic 35.0%
TTTCTCACATAAGAGTCAAG+AGG + chr7_1:751223-751242 None:intergenic 35.0%
! CTGATTGCATGATAGTGTAA+TGG + chr7_1:751312-751331 None:intergenic 35.0%
AGCTCAAAAGGCAATAAAAG+GGG + chr7_1:751428-751447 None:intergenic 35.0%
GAGCTCAAAAGGCAATAAAA+GGG + chr7_1:751429-751448 None:intergenic 35.0%
AGAGCTCAAAAGGCAATAAA+AGG + chr7_1:751430-751449 None:intergenic 35.0%
TTACTGAATGTAGATAAGCC+TGG - chr7_1:751490-751509 Msa1003680:intron 35.0%
TGTGTTATCACTGACATTCA+TGG - chr7_1:751581-751600 Msa1003680:intron 35.0%
GTGTTATCACTGACATTCAT+GGG - chr7_1:751582-751601 Msa1003680:intron 35.0%
! ATGTATTGATGCAATGTTCC+GGG + chr7_1:751760-751779 None:intergenic 35.0%
! GATGTATTGATGCAATGTTC+CGG + chr7_1:751761-751780 None:intergenic 35.0%
GGATGTATTGCTTATGAATG+AGG - chr7_1:752125-752144 Msa1003680:intron 35.0%
TTTCAATCCCATGGAAACAA+TGG + chr7_1:752169-752188 None:intergenic 35.0%
!! CATGTTTGTTTTCTCCACAA+AGG - chr7_1:752209-752228 Msa1003680:five_prime_UTR 35.0%
! TTTGAAGCAAAAGCACAAAC+AGG + chr7_1:749979-749998 None:intergenic 35.0%
ATTCAGCACAATAGGAAAGT+GGG + chr7_1:750524-750543 None:intergenic 35.0%
AATTCAGCACAATAGGAAAG+TGG + chr7_1:750525-750544 None:intergenic 35.0%
ATAGATGCTGTAAAAAGCAG+GGG - chr7_1:750898-750917 Msa1003680:intron 35.0%
CCACAAGATTGTGTACTTAT+TGG + chr7_1:751053-751072 None:intergenic 35.0%
CCAATAAGTACACAATCTTG+TGG - chr7_1:751050-751069 Msa1003680:intron 35.0%
!! CTTCTCTAAGATGACTATGT+TGG - chr7_1:751148-751167 Msa1003680:intron 35.0%
GAGTCAAGAGGAACAAAATT+AGG + chr7_1:751211-751230 None:intergenic 35.0%
TTTCTCACATAAGAGTCAAG+AGG + chr7_1:751223-751242 None:intergenic 35.0%
! CTGATTGCATGATAGTGTAA+TGG + chr7_1:751312-751331 None:intergenic 35.0%
AGCTCAAAAGGCAATAAAAG+GGG + chr7_1:751428-751447 None:intergenic 35.0%
GAGCTCAAAAGGCAATAAAA+GGG + chr7_1:751429-751448 None:intergenic 35.0%
AGAGCTCAAAAGGCAATAAA+AGG + chr7_1:751430-751449 None:intergenic 35.0%
TTACTGAATGTAGATAAGCC+TGG - chr7_1:751490-751509 Msa1003680:intron 35.0%
TGTGTTATCACTGACATTCA+TGG - chr7_1:751581-751600 Msa1003680:intron 35.0%
GTGTTATCACTGACATTCAT+GGG - chr7_1:751582-751601 Msa1003680:intron 35.0%
! ATGTATTGATGCAATGTTCC+GGG + chr7_1:751760-751779 None:intergenic 35.0%
! GATGTATTGATGCAATGTTC+CGG + chr7_1:751761-751780 None:intergenic 35.0%
GGATGTATTGCTTATGAATG+AGG - chr7_1:752125-752144 Msa1003680:intron 35.0%
TTTCAATCCCATGGAAACAA+TGG + chr7_1:752169-752188 None:intergenic 35.0%
!! CATGTTTGTTTTCTCCACAA+AGG - chr7_1:752209-752228 Msa1003680:five_prime_UTR 35.0%
ACTGGACAAGACAAAGGAAA+AGG - chr7_1:749827-749846 Msa1003680:three_prime_UTR 40.0%
CTGGACAAGACAAAGGAAAA+GGG - chr7_1:749828-749847 Msa1003680:three_prime_UTR 40.0%
! CTGATGATGAAGTTAGGGTT+TGG + chr7_1:749909-749928 None:intergenic 40.0%
TCTCTCTATTGGATTTCTGC+TGG - chr7_1:750022-750041 Msa1003680:three_prime_UTR 40.0%
CTTTATACAGGTTGTTTGGC+TGG - chr7_1:750088-750107 Msa1003680:CDS 40.0%
ATGCACAGGATGAAACATGT+GGG - chr7_1:750268-750287 Msa1003680:intron 40.0%
ATGAAACATGTGGGATCTGT+AGG - chr7_1:750277-750296 Msa1003680:intron 40.0%
TGTCCTGATTGTAAACTTCC+TGG - chr7_1:750318-750337 Msa1003680:intron 40.0%
GTCCTGATTGTAAACTTCCT+GGG - chr7_1:750319-750338 Msa1003680:intron 40.0%
TCCTGATTGTAAACTTCCTG+GGG - chr7_1:750320-750339 Msa1003680:intron 40.0%
CTGAGCTGAAGAAAAGTAGT+AGG - chr7_1:750929-750948 Msa1003680:intron 40.0%
CTGCATGTAAAGAGCTCAAA+AGG + chr7_1:751440-751459 None:intergenic 40.0%
! CTTTTGAGCTCTTTACATGC+AGG - chr7_1:751438-751457 Msa1003680:intron 40.0%
AAAGGAGGAAGACATCAAAG+TGG + chr7_1:751736-751755 None:intergenic 40.0%
! TGTATTGATGCAATGTTCCG+GGG + chr7_1:751759-751778 None:intergenic 40.0%
! AAACTGCACATAGTGGGAAA+AGG + chr7_1:751882-751901 None:intergenic 40.0%
! TTTTCCCACTATGTGCAGTT+TGG - chr7_1:751881-751900 Msa1003680:intron 40.0%
TTTCCCACTATGTGCAGTTT+GGG - chr7_1:751882-751901 Msa1003680:intron 40.0%
ACTGGACAAGACAAAGGAAA+AGG - chr7_1:749827-749846 Msa1003680:three_prime_UTR 40.0%
CTGGACAAGACAAAGGAAAA+GGG - chr7_1:749828-749847 Msa1003680:three_prime_UTR 40.0%
! CTGATGATGAAGTTAGGGTT+TGG + chr7_1:749909-749928 None:intergenic 40.0%
TCTCTCTATTGGATTTCTGC+TGG - chr7_1:750022-750041 Msa1003680:three_prime_UTR 40.0%
CTTTATACAGGTTGTTTGGC+TGG - chr7_1:750088-750107 Msa1003680:CDS 40.0%
ATGCACAGGATGAAACATGT+GGG - chr7_1:750268-750287 Msa1003680:intron 40.0%
ATGAAACATGTGGGATCTGT+AGG - chr7_1:750277-750296 Msa1003680:intron 40.0%
TGTCCTGATTGTAAACTTCC+TGG - chr7_1:750318-750337 Msa1003680:intron 40.0%
GTCCTGATTGTAAACTTCCT+GGG - chr7_1:750319-750338 Msa1003680:intron 40.0%
TCCTGATTGTAAACTTCCTG+GGG - chr7_1:750320-750339 Msa1003680:intron 40.0%
CTGAGCTGAAGAAAAGTAGT+AGG - chr7_1:750929-750948 Msa1003680:intron 40.0%
CTGCATGTAAAGAGCTCAAA+AGG + chr7_1:751440-751459 None:intergenic 40.0%
! CTTTTGAGCTCTTTACATGC+AGG - chr7_1:751438-751457 Msa1003680:intron 40.0%
AAAGGAGGAAGACATCAAAG+TGG + chr7_1:751736-751755 None:intergenic 40.0%
! TGTATTGATGCAATGTTCCG+GGG + chr7_1:751759-751778 None:intergenic 40.0%
! AAACTGCACATAGTGGGAAA+AGG + chr7_1:751882-751901 None:intergenic 40.0%
! TTTTCCCACTATGTGCAGTT+TGG - chr7_1:751881-751900 Msa1003680:intron 40.0%
TTTCCCACTATGTGCAGTTT+GGG - chr7_1:751882-751901 Msa1003680:intron 40.0%
! ATGCTGTTGCTTCTTGGACT+TGG - chr7_1:750244-750263 Msa1003680:intron 45.0%
! TGCTGTTGCTTCTTGGACTT+GGG - chr7_1:750245-750264 Msa1003680:intron 45.0%
GATGCACAGGATGAAACATG+TGG - chr7_1:750267-750286 Msa1003680:intron 45.0%
AACATGTGGGATCTGTAGGA+TGG - chr7_1:750281-750300 Msa1003680:intron 45.0%
ATCTGTAGGATGGCCTTTGA+TGG - chr7_1:750291-750310 Msa1003680:intron 45.0%
ATCAGGACAGCATCCATCAA+AGG + chr7_1:750307-750326 None:intergenic 45.0%
TCCCCAGGAAGTTTACAATC+AGG + chr7_1:750324-750343 None:intergenic 45.0%
CACTAGTGAGAATGCAGTTG+AGG + chr7_1:750486-750505 None:intergenic 45.0%
CAATAGGAAAGTGGGCAAAC+AGG + chr7_1:750516-750535 None:intergenic 45.0%
TTGACTGAGACGTGGAACAA+AGG + chr7_1:751007-751026 None:intergenic 45.0%
!! ATATCGACTTGACTGAGACG+TGG + chr7_1:751015-751034 None:intergenic 45.0%
CGTCTCAGTCAAGTCGATAT+AGG - chr7_1:751015-751034 Msa1003680:intron 45.0%
CTATCATGCAATCAGCCAAC+TGG - chr7_1:751317-751336 Msa1003680:intron 45.0%
AGGGGAACTCATTGTGTACA+AGG + chr7_1:751410-751429 None:intergenic 45.0%
TTCCCACTATGTGCAGTTTG+GGG - chr7_1:751883-751902 Msa1003680:intron 45.0%
CGTCGGGAATGGCAATTTAA+AGG - chr7_1:751988-752007 Msa1003680:intron 45.0%
! ATGCTGTTGCTTCTTGGACT+TGG - chr7_1:750244-750263 Msa1003680:intron 45.0%
! TGCTGTTGCTTCTTGGACTT+GGG - chr7_1:750245-750264 Msa1003680:intron 45.0%
GATGCACAGGATGAAACATG+TGG - chr7_1:750267-750286 Msa1003680:intron 45.0%
AACATGTGGGATCTGTAGGA+TGG - chr7_1:750281-750300 Msa1003680:intron 45.0%
ATCTGTAGGATGGCCTTTGA+TGG - chr7_1:750291-750310 Msa1003680:intron 45.0%
ATCAGGACAGCATCCATCAA+AGG + chr7_1:750307-750326 None:intergenic 45.0%
TCCCCAGGAAGTTTACAATC+AGG + chr7_1:750324-750343 None:intergenic 45.0%
CACTAGTGAGAATGCAGTTG+AGG + chr7_1:750486-750505 None:intergenic 45.0%
CAATAGGAAAGTGGGCAAAC+AGG + chr7_1:750516-750535 None:intergenic 45.0%
TTGACTGAGACGTGGAACAA+AGG + chr7_1:751007-751026 None:intergenic 45.0%
!! ATATCGACTTGACTGAGACG+TGG + chr7_1:751015-751034 None:intergenic 45.0%
CGTCTCAGTCAAGTCGATAT+AGG - chr7_1:751015-751034 Msa1003680:intron 45.0%
CTATCATGCAATCAGCCAAC+TGG - chr7_1:751317-751336 Msa1003680:intron 45.0%
AGGGGAACTCATTGTGTACA+AGG + chr7_1:751410-751429 None:intergenic 45.0%
TTCCCACTATGTGCAGTTTG+GGG - chr7_1:751883-751902 Msa1003680:intron 45.0%
CGTCGGGAATGGCAATTTAA+AGG - chr7_1:751988-752007 Msa1003680:intron 45.0%
TACAGGTTGTTTGGCTGGTG+TGG - chr7_1:750093-750112 Msa1003680:CDS 50.0%
TTGTTTGGCTGGTGTGGTTC+TGG - chr7_1:750099-750118 Msa1003680:CDS 50.0%
! GATGGCATGCTGTTGCTTCT+TGG - chr7_1:750238-750257 Msa1003680:intron 50.0%
TTCTTGGACTTGGGATGCAC+AGG - chr7_1:750254-750273 Msa1003680:intron 50.0%
TGATGTCTTCCTCCTTTCCC+CGG - chr7_1:751739-751758 Msa1003680:CDS 50.0%
TGATGCAATGTTCCGGGGAA+AGG + chr7_1:751754-751773 None:intergenic 50.0%
CACCCCAAACTGCACATAGT+GGG + chr7_1:751888-751907 None:intergenic 50.0%
CATTGGGCAATGTGCTTGTG+AGG + chr7_1:751965-751984 None:intergenic 50.0%
CACATTGCCCAATGTGTCGT+CGG - chr7_1:751971-751990 Msa1003680:intron 50.0%
ACATTGCCCAATGTGTCGTC+GGG - chr7_1:751972-751991 Msa1003680:intron 50.0%
TACAGGTTGTTTGGCTGGTG+TGG - chr7_1:750093-750112 Msa1003680:CDS 50.0%
TTGTTTGGCTGGTGTGGTTC+TGG - chr7_1:750099-750118 Msa1003680:CDS 50.0%
! GATGGCATGCTGTTGCTTCT+TGG - chr7_1:750238-750257 Msa1003680:intron 50.0%
TTCTTGGACTTGGGATGCAC+AGG - chr7_1:750254-750273 Msa1003680:intron 50.0%
TGATGTCTTCCTCCTTTCCC+CGG - chr7_1:751739-751758 Msa1003680:CDS 50.0%
TGATGCAATGTTCCGGGGAA+AGG + chr7_1:751754-751773 None:intergenic 50.0%
CACCCCAAACTGCACATAGT+GGG + chr7_1:751888-751907 None:intergenic 50.0%
CATTGGGCAATGTGCTTGTG+AGG + chr7_1:751965-751984 None:intergenic 50.0%
CACATTGCCCAATGTGTCGT+CGG - chr7_1:751971-751990 Msa1003680:intron 50.0%
ACATTGCCCAATGTGTCGTC+GGG - chr7_1:751972-751991 Msa1003680:intron 50.0%
TGCGGGACTGGACAAGACAA+AGG - chr7_1:749821-749840 Msa1003680:three_prime_UTR 55.0%
TGAGACGTGGAACAAAGGCC+TGG + chr7_1:751002-751021 None:intergenic 55.0%
TGCAATGTTCCGGGGAAAGG+AGG + chr7_1:751751-751770 None:intergenic 55.0%
GCACCCCAAACTGCACATAG+TGG + chr7_1:751889-751908 None:intergenic 55.0%
GCCATTCCCGACGACACATT+GGG + chr7_1:751981-752000 None:intergenic 55.0%
TGCCATTCCCGACGACACAT+TGG + chr7_1:751982-752001 None:intergenic 55.0%
TGCGGGACTGGACAAGACAA+AGG - chr7_1:749821-749840 Msa1003680:three_prime_UTR 55.0%
TGAGACGTGGAACAAAGGCC+TGG + chr7_1:751002-751021 None:intergenic 55.0%
TGCAATGTTCCGGGGAAAGG+AGG + chr7_1:751751-751770 None:intergenic 55.0%
GCACCCCAAACTGCACATAG+TGG + chr7_1:751889-751908 None:intergenic 55.0%
GCCATTCCCGACGACACATT+GGG + chr7_1:751981-752000 None:intergenic 55.0%
TGCCATTCCCGACGACACAT+TGG + chr7_1:751982-752001 None:intergenic 55.0%
CTGAGTGGACAGTCATCCCC+AGG + chr7_1:750339-750358 None:intergenic 60.0%
GCCCAATGTGTCGTCGGGAA+TGG - chr7_1:751977-751996 Msa1003680:intron 60.0%
CTGAGTGGACAGTCATCCCC+AGG + chr7_1:750339-750358 None:intergenic 60.0%
GCCCAATGTGTCGTCGGGAA+TGG - chr7_1:751977-751996 Msa1003680:intron 60.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 749813 752263 749813 ID=Msa1003680;Name=Msa1003680
chr7_1 mRNA 749813 752263 749813 ID=Msa1003680-mRNA-1;Parent=Msa1003680;Name=Msa1003680-mRNA-1;_AED=0.08;_eAED=0.08;_QI=119|0.5|0.66|1|0|0|3|249|102
chr7_1 exon 749813 750177 749813 ID=Msa1003680-mRNA-1:exon:249;Parent=Msa1003680-mRNA-1
chr7_1 exon 751664 751837 751664 ID=Msa1003680-mRNA-1:exon:248;Parent=Msa1003680-mRNA-1
chr7_1 exon 752126 752263 752126 ID=Msa1003680-mRNA-1:exon:247;Parent=Msa1003680-mRNA-1
chr7_1 five_prime_UTR 752145 752263 752145 ID=Msa1003680-mRNA-1:five_prime_utr;Parent=Msa1003680-mRNA-1
chr7_1 CDS 752126 752144 752126 ID=Msa1003680-mRNA-1:cds;Parent=Msa1003680-mRNA-1
chr7_1 CDS 751664 751837 751664 ID=Msa1003680-mRNA-1:cds;Parent=Msa1003680-mRNA-1
chr7_1 CDS 750062 750177 750062 ID=Msa1003680-mRNA-1:cds;Parent=Msa1003680-mRNA-1
chr7_1 three_prime_UTR 749813 750061 749813 ID=Msa1003680-mRNA-1:three_prime_utr;Parent=Msa1003680-mRNA-1
Gene Sequence

>Msa1003680

ATGAAAGTCAATATCTTGCATGGCATGCTGTTGCTTCTTGGACTTGGGATGCACAGGATGAAACATGTGGGATCTGTAGGATGGCCTTTGATGGATGCTGTCCTGATTGTAAACTTCCTGGGGATGACTGTCCACTCAGTAAGTTACATTATCTTGGGATTTTGCATACTTGTTATTATATTTGCATACAATTTTTGGGGTGCATGCAATCACGCATTTCATTTACATTGTATCTTGAAATGGGTGAATTCTCAAACCTCACAAGCACATTGCCCAATGTGTCGTCGGGAATGGCAATTTAAAGGTTGA
Protein sequence

>Msa1003680

MKVNILHGMLLLLGLGMHRMKHVGSVGWPLMDAVLIVNFLGMTVHSVSYIILGFCILVIIFAYNFWGACNHAFHLHCILKWVNSQTSQAHCPMCRREWQFKG*