AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1007840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1007840 MtrunA17_Chr7g0218101 98.282 291 5 0 1 291 1 291 0.0 590
Msa1007840 MtrunA17_Chr3g0077201 44.939 247 121 7 49 288 83 321 4.71e-57 186
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1007840 AT3G29240.1 61.093 311 95 7 1 286 1 310 1.46e-120 348
Msa1007840 AT3G29240.2 61.093 311 95 7 1 286 1 310 1.46e-120 348
Msa1007840 AT1G33780.1 46.552 232 105 4 59 286 95 311 1.85e-56 185
Msa1007840 AT3G19780.1 32.258 155 87 5 114 259 904 1049 5.55e-12 66.6
Msa1007840 AT3G19780.2 32.258 155 87 5 114 259 903 1048 5.66e-12 66.6
Msa1007840 AT3G19780.4 32.258 155 87 5 114 259 712 857 6.59e-12 66.2
Msa1007840 AT3G19780.3 32.258 155 87 5 114 259 694 839 7.05e-12 66.2

Find 128 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAACAGTCCTTTGTATTTC+GGG 0.163087 7_1:+7567218 Msa1007840:CDS
TTCAAGTTACTATTCAAATT+AGG 0.177850 7_1:-7567907 None:intergenic
ATTAATAAACAACCTCTTTC+GGG 0.199516 7_1:-7567034 None:intergenic
AACAATTGAGAGATGAAATT+AGG 0.215233 7_1:+7567440 Msa1007840:CDS
TCTAACAGTCCTTTGTATTT+CGG 0.220455 7_1:+7567217 Msa1007840:CDS
TTCGGCTATATTTGAATTGA+TGG 0.264318 7_1:+7567779 Msa1007840:three_prime_UTR
TCACTGTTCGTTCGAAAATA+TGG 0.265431 7_1:-7567081 None:intergenic
ATGTTGGAATTGTTGGGCTC+TGG 0.270864 7_1:+7567515 Msa1007840:CDS
TTGGGGAATGTTGGAATTGT+TGG 0.278049 7_1:+7567508 Msa1007840:CDS
ATGAAATTAGGGATGGTTAT+TGG 0.278275 7_1:+7567452 Msa1007840:CDS
GGACGAAGTTCTCGGGCTTA+TGG 0.278397 7_1:+7567537 Msa1007840:CDS
TATTAATAAACAACCTCTTT+CGG 0.280157 7_1:-7567035 None:intergenic
TTTCCCACCACATCATCTTT+TGG 0.289279 7_1:-7567274 None:intergenic
ACAAAGTTACTCAAATAATA+TGG 0.298640 7_1:+7565917 Msa1007840:exon
TCAAGTTACTATTCAAATTA+GGG 0.300355 7_1:-7567906 None:intergenic
GATAAAACAATGTTTATGTT+TGG 0.310889 7_1:+7567656 Msa1007840:three_prime_UTR
GTGATGAAGGGTTTGTATTA+TGG 0.321373 7_1:+7567316 Msa1007840:CDS
ACAATTGAGAGATGAAATTA+GGG 0.325075 7_1:+7567441 Msa1007840:CDS
ACAACTTGTTTGGAAGCTAT+TGG 0.331561 7_1:+7567931 Msa1007840:three_prime_UTR
AGTCCAAGTGTTGTTGATTT+GGG 0.333914 7_1:+7567490 Msa1007840:CDS
ATGATCCCTGATGGGCCTAT+TGG 0.337536 7_1:-7567124 None:intergenic
TTTGAAATTGCATCGCATAT+TGG 0.341950 7_1:+7568138 Msa1007840:three_prime_UTR
TAACTTGAAAACAACTTGTT+TGG 0.342908 7_1:+7567921 Msa1007840:three_prime_UTR
AAGATCGCCAATCGGTGTTA+AGG 0.343777 7_1:-7566877 None:intergenic
TAGTCCAAGTGTTGTTGATT+TGG 0.347343 7_1:+7567489 Msa1007840:CDS
TGGGGAATGTTGGAATTGTT+GGG 0.353274 7_1:+7567509 Msa1007840:CDS
GGGCTCTGGGACGAAGTTCT+CGG 0.353820 7_1:+7567529 Msa1007840:CDS
TCTTCATCTCATCCAAAGTT+TGG 0.355676 7_1:-7565774 None:intergenic
GATGTTAACTATGAGAATTT+TGG 0.357996 7_1:+7568059 Msa1007840:three_prime_UTR
GACGAAGTTCTCGGGCTTAT+GGG 0.359750 7_1:+7567538 Msa1007840:CDS
ATCAACTGCATTTGATGTAA+TGG 0.365972 7_1:+7567186 Msa1007840:CDS
TAATCACAATTGGTGACAAA+TGG 0.368700 7_1:+7566993 Msa1007840:CDS
TATTAAAATGTATGGGCTAA+TGG 0.370968 7_1:-7565747 None:intergenic
AACGTGAGGGTGGGTAAAAT+CGG 0.381314 7_1:-7567598 None:intergenic
AACAACTAAACACAACAATT+AGG 0.390992 7_1:-7568032 None:intergenic
AGTCGCTGAAGAAAGGAATT+CGG 0.391351 7_1:-7566939 None:intergenic
AGTTTGGTGGAATATGTATA+TGG 0.391750 7_1:-7565855 None:intergenic
GGGCACTAGTATTAACCCTT+AGG 0.394769 7_1:-7567886 None:intergenic
TTGGTGTGTATTAAAATGTA+TGG 0.399837 7_1:-7565755 None:intergenic
GTCGCTGAAGAAAGGAATTC+GGG 0.401772 7_1:-7566938 None:intergenic
GCGATCTTTCCGTGCAAAAC+TGG 0.403195 7_1:+7566892 Msa1007840:CDS
TGATCCCTGATGGGCCTATT+GGG 0.409481 7_1:-7567123 None:intergenic
TGTTGGAATTGTTGGGCTCT+GGG 0.418680 7_1:+7567516 Msa1007840:CDS
AACTTACATGATACATGAAA+TGG 0.420587 7_1:-7566047 None:intergenic
CTTTGATGGGTATTGTGGGT+GGG 0.424635 7_1:+7567411 Msa1007840:CDS
CAATTCAATTCAAGAAAAGT+TGG 0.426906 7_1:+7566020 Msa1007840:CDS
GGCTCTGGGACGAAGTTCTC+GGG 0.430974 7_1:+7567530 Msa1007840:CDS
AACAACCTCTTTCGGGCTCA+TGG 0.431253 7_1:-7567027 None:intergenic
AATAAGCTTGTAGAAGATCT+AGG 0.431841 7_1:+7567849 Msa1007840:three_prime_UTR
TTGTTGATGAGAAAATCTTT+TGG 0.440379 7_1:-7566000 None:intergenic
AATGGATATGCTTTGACAAA+TGG 0.441277 7_1:-7565729 None:intergenic
TCAACTGCATTTGATGTAAT+GGG 0.441472 7_1:+7567187 Msa1007840:CDS
TGTGGGTCTTGCTGCTGAAA+TGG 0.442545 7_1:+7567351 Msa1007840:CDS
TTGTCACCAATTGTGATTAG+TGG 0.444671 7_1:-7566989 None:intergenic
AGGGTTGGTTTGTCATCATC+CGG 0.446445 7_1:-7566857 None:intergenic
GGATGATTCACAACAGTGTC+TGG 0.452781 7_1:-7566965 None:intergenic
ATATGATGAGAGATCAAGTT+TGG 0.453059 7_1:-7565871 None:intergenic
CTTGTGGGGATGGGAAAAGT+TGG 0.458568 7_1:+7567697 Msa1007840:three_prime_UTR
TAGAGATCCACCTCTGATCT+TGG 0.458828 7_1:-7565970 None:intergenic
AGATCGCCAATCGGTGTTAA+GGG 0.458925 7_1:-7566876 None:intergenic
AGTAGATACATCCATCCTTC+AGG 0.463640 7_1:-7568107 None:intergenic
CGATTCTTTGATGGGTATTG+TGG 0.467006 7_1:+7567406 Msa1007840:CDS
CATCCACCACTAATCACAAT+TGG 0.467643 7_1:+7566983 Msa1007840:CDS
CGCCAATCGGTGTTAAGGGT+TGG 0.469028 7_1:-7566872 None:intergenic
GGTGTAGTAGCAAGACCCTA+AGG 0.472149 7_1:+7567870 Msa1007840:three_prime_UTR
TTTGGAAGCTATTGGCAAGC+AGG 0.478143 7_1:+7567939 Msa1007840:three_prime_UTR
TTCTCGGGCTTATGGGCAAA+AGG 0.480614 7_1:+7567545 Msa1007840:CDS
GTTGTTGATTTGGGGAATGT+TGG 0.484466 7_1:+7567499 Msa1007840:CDS
TTGTGGGGATGGGAAAAGTT+GGG 0.487517 7_1:+7567698 Msa1007840:three_prime_UTR
TTGGGCCGTTTGATAAAAGA+AGG 0.489401 7_1:-7567105 None:intergenic
AATCACAATTGGTGACAAAT+GGG 0.489815 7_1:+7566994 Msa1007840:CDS
GGCACTAGTATTAACCCTTA+GGG 0.491646 7_1:-7567885 None:intergenic
GATTCTTTGATGGGTATTGT+GGG 0.492753 7_1:+7567407 Msa1007840:CDS
TAGCAACAGAAAAGCTTGAT+GGG 0.499250 7_1:+7567056 Msa1007840:CDS
AAGAGGAATGTGGTCGAGGT+TGG 0.500640 7_1:+7567376 Msa1007840:CDS
CTGGATCAGTCGCTGAAGAA+AGG 0.501880 7_1:-7566946 None:intergenic
TACACATGAAGAGTGCTTGT+GGG 0.507617 7_1:+7567682 Msa1007840:three_prime_UTR
ATAGCAACAGAAAAGCTTGA+TGG 0.509500 7_1:+7567055 Msa1007840:CDS
ACGTGAGGGTGGGTAAAATC+GGG 0.514202 7_1:-7567597 None:intergenic
ATGAAGAGTGCTTGTGGGGA+TGG 0.514958 7_1:+7567687 Msa1007840:three_prime_UTR
TGCACGGAAAGATCGCCAAT+CGG 0.515533 7_1:-7566885 None:intergenic
TCTTTGATAGACATCAAAGA+TGG 0.519470 7_1:-7567157 None:intergenic
AACCAACCCTTAACACCGAT+TGG 0.525116 7_1:+7566870 Msa1007840:CDS
TTCCCCAAATCAACAACACT+TGG 0.527843 7_1:-7567493 None:intergenic
TTCAAAATGTCTAATCCTGA+AGG 0.531792 7_1:+7568092 Msa1007840:three_prime_UTR
TTCCGTGCAAAACTGGTGGC+CGG 0.536600 7_1:+7566899 Msa1007840:CDS
GGCTCTAATTGAAAGGGTAC+CGG 0.542888 7_1:+7567719 Msa1007840:three_prime_UTR
GTAATCCATGAGCCCGAAAG+AGG 0.546559 7_1:+7567022 Msa1007840:CDS
TCTTTGATGGGTATTGTGGG+TGG 0.550755 7_1:+7567410 Msa1007840:CDS
AAAGTTGGGCTCTAATTGAA+AGG 0.551613 7_1:+7567712 Msa1007840:three_prime_UTR
TAACAGTCCTTTGTATTTCG+GGG 0.552255 7_1:+7567219 Msa1007840:CDS
TGATGAGAGATCAAGTTTGG+TGG 0.559897 7_1:-7565868 None:intergenic
TCACCAATTGTGATTAGTGG+TGG 0.561176 7_1:-7566986 None:intergenic
CTATTGAGTATGATCCCTGA+TGG 0.565216 7_1:-7567133 None:intergenic
ATCTTTCCGTGCAAAACTGG+TGG 0.565955 7_1:+7566895 Msa1007840:CDS
AAATGTCTAATCCTGAAGGA+TGG 0.573292 7_1:+7568096 Msa1007840:three_prime_UTR
AAGTTGGGCTCTAATTGAAA+GGG 0.573938 7_1:+7567713 Msa1007840:three_prime_UTR
TATTGAGTATGATCCCTGAT+GGG 0.580509 7_1:-7567132 None:intergenic
TGGTGTGTATTAAAATGTAT+GGG 0.582839 7_1:-7565754 None:intergenic
TGAAGAGTGCTTGTGGGGAT+GGG 0.584834 7_1:+7567688 Msa1007840:three_prime_UTR
TTTCGAGGAAACGTGAGGGT+GGG 0.587065 7_1:-7567607 None:intergenic
TCCACCTCTGATCTTGGTGA+AGG 0.588907 7_1:-7565964 None:intergenic
TCCTTCACCAAGATCAGAGG+TGG 0.589140 7_1:+7565963 Msa1007840:CDS
AACAGTCCTTTGTATTTCGG+GGG 0.595092 7_1:+7567220 Msa1007840:CDS
TTGAGAGATGAAATTAGGGA+TGG 0.595243 7_1:+7567445 Msa1007840:CDS
GGTGAAGAGGAATGTGGTCG+AGG 0.602124 7_1:+7567372 Msa1007840:CDS
TTATGGTACTAAAGAAAGTG+TGG 0.602423 7_1:+7567333 Msa1007840:CDS
TTGCTGCTGAAATGGTGAAG+AGG 0.609923 7_1:+7567359 Msa1007840:CDS
ATACACATGAAGAGTGCTTG+TGG 0.618094 7_1:+7567681 Msa1007840:three_prime_UTR
CATGTCATCATCATCACCAC+CGG 0.618724 7_1:+7566838 Msa1007840:CDS
GAGAACATGAGTGTCAATAC+CGG 0.618757 7_1:-7567738 None:intergenic
TGCTTTGACAAATGGCACAT+AGG 0.619813 7_1:-7565721 None:intergenic
GTCCAAGTGTTGTTGATTTG+GGG 0.620298 7_1:+7567491 Msa1007840:CDS
GTGTAGTAGCAAGACCCTAA+GGG 0.624591 7_1:+7567871 Msa1007840:three_prime_UTR
TATGGTACTAAAGAAAGTGT+GGG 0.628740 7_1:+7567334 Msa1007840:CDS
AGTTCCTTCACCAAGATCAG+AGG 0.637714 7_1:+7565960 Msa1007840:CDS
GTTGGTTTGTCATCATCCGG+TGG 0.639856 7_1:-7566854 None:intergenic
ACGGCCCAATAGGCCCATCA+GGG 0.647590 7_1:+7567119 Msa1007840:CDS
ATATTTGAATTGATGGCATG+TGG 0.651797 7_1:+7567786 Msa1007840:three_prime_UTR
AGTGAGTCCAAAAGATGATG+TGG 0.656476 7_1:+7567267 Msa1007840:CDS
ACATACAAAATACTTCTATG+TGG 0.659206 7_1:+7565803 Msa1007840:five_prime_UTR
AGCAACAGAAAAGCTTGATG+GGG 0.667622 7_1:+7567057 Msa1007840:CDS
GAGTCCAAAAGATGATGTGG+TGG 0.670451 7_1:+7567270 Msa1007840:CDS
TTAATCTCTAAAGTTACACA+CGG 0.678868 7_1:-7568192 None:intergenic
ACACATGAAGAGTGCTTGTG+GGG 0.684509 7_1:+7567683 Msa1007840:three_prime_UTR
GGCAAAAGGAAAGTCAAGTG+AGG 0.702168 7_1:+7567559 Msa1007840:CDS
TGAAATGGTGAAGAGGAATG+TGG 0.726049 7_1:+7567366 Msa1007840:CDS
AGTCCAAAAGATGATGTGGT+GGG 0.732496 7_1:+7567271 Msa1007840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATAAAATATGTGAATT+TGG + chr7_1:7566475-7566494 Msa1007840:intron 10.0%
!!! TATATTGATAATGATACTTT+AGG + chr7_1:7566520-7566539 Msa1007840:intron 15.0%
!!! ATATTGATAATGATACTTTA+GGG + chr7_1:7566521-7566540 Msa1007840:intron 15.0%
!! ACAAAGTTACTCAAATAATA+TGG + chr7_1:7565917-7565936 Msa1007840:exon 20.0%
!! TGTAAAATATTGTAGTTACT+AGG + chr7_1:7566324-7566343 Msa1007840:intron 20.0%
!! TATAATTTAGAAGCTAAGTT+GGG - chr7_1:7566434-7566453 None:intergenic 20.0%
!! ATATAATTTAGAAGCTAAGT+TGG - chr7_1:7566435-7566454 None:intergenic 20.0%
!!! ATTATATTGATTGTACATTC+AGG + chr7_1:7566544-7566563 Msa1007840:intron 20.0%
!! TAAAAAACCGAATTTCTATA+TGG - chr7_1:7566605-7566624 None:intergenic 20.0%
!! TATTAATAAACAACCTCTTT+CGG - chr7_1:7567038-7567057 None:intergenic 20.0%
!! GATAAAACAATGTTTATGTT+TGG + chr7_1:7567656-7567675 Msa1007840:three_prime_UTR 20.0%
!! TCAAGTTACTATTCAAATTA+GGG - chr7_1:7567909-7567928 None:intergenic 20.0%
!!! TTCAAGTTACTATTCAAATT+AGG - chr7_1:7567910-7567929 None:intergenic 20.0%
!!! GATTCAATTTTAGTGTAATT+TGG + chr7_1:7568162-7568181 Msa1007840:three_prime_UTR 20.0%
!!! AATTTTTTAGAGTTGTGTTA+AGG + chr7_1:7568214-7568233 Msa1007840:three_prime_UTR 20.0%
!! TATTAAAATGTATGGGCTAA+TGG - chr7_1:7565750-7565769 None:intergenic 25.0%
! TGGTGTGTATTAAAATGTAT+GGG - chr7_1:7565757-7565776 None:intergenic 25.0%
! TTGGTGTGTATTAAAATGTA+TGG - chr7_1:7565758-7565777 None:intergenic 25.0%
!! TTTTAATACACACCAAACTT+TGG + chr7_1:7565762-7565781 Msa1007840:five_prime_UTR 25.0%
! ACATACAAAATACTTCTATG+TGG + chr7_1:7565803-7565822 Msa1007840:five_prime_UTR 25.0%
!!! TTGTTGATGAGAAAATCTTT+TGG - chr7_1:7566003-7566022 None:intergenic 25.0%
! CAATTCAATTCAAGAAAAGT+TGG + chr7_1:7566020-7566039 Msa1007840:CDS 25.0%
! AACTTACATGATACATGAAA+TGG - chr7_1:7566050-7566069 None:intergenic 25.0%
! AAACAATTGTAGTGAAATTG+AGG + chr7_1:7566172-7566191 Msa1007840:intron 25.0%
! ATAATTTAGAAGCTAAGTTG+GGG - chr7_1:7566433-7566452 None:intergenic 25.0%
! TGTTGTACCATATAGAAATT+CGG + chr7_1:7566595-7566614 Msa1007840:intron 25.0%
! ATTAATAAACAACCTCTTTC+GGG - chr7_1:7567037-7567056 None:intergenic 25.0%
! AACAATTGAGAGATGAAATT+AGG + chr7_1:7567440-7567459 Msa1007840:CDS 25.0%
! ACAATTGAGAGATGAAATTA+GGG + chr7_1:7567441-7567460 Msa1007840:CDS 25.0%
!! TAACTTGAAAACAACTTGTT+TGG + chr7_1:7567921-7567940 Msa1007840:three_prime_UTR 25.0%
! AACAACTAAACACAACAATT+AGG - chr7_1:7568035-7568054 None:intergenic 25.0%
!! GATGTTAACTATGAGAATTT+TGG + chr7_1:7568059-7568078 Msa1007840:three_prime_UTR 25.0%
!!! TTAATCTCTAAAGTTACACA+CGG - chr7_1:7568195-7568214 None:intergenic 25.0%
AATGGATATGCTTTGACAAA+TGG - chr7_1:7565732-7565751 None:intergenic 30.0%
! AGTTTGGTGGAATATGTATA+TGG - chr7_1:7565858-7565877 None:intergenic 30.0%
ATATGATGAGAGATCAAGTT+TGG - chr7_1:7565874-7565893 None:intergenic 30.0%
!!! TTTGTTTTTAGATGAGAGTG+AGG - chr7_1:7565902-7565921 None:intergenic 30.0%
!!! TGAGAAAATCTTTTGGAGTA+AGG - chr7_1:7565996-7566015 None:intergenic 30.0%
ATCTTGATCATAGTGTAAGT+AGG - chr7_1:7566082-7566101 None:intergenic 30.0%
! TAATTTAGAAGCTAAGTTGG+GGG - chr7_1:7566432-7566451 None:intergenic 30.0%
!!! GAAATTCGGTTTTTTACCAT+TGG + chr7_1:7566609-7566628 Msa1007840:intron 30.0%
! TTTTCACCATCTATAAGTCT+CGG - chr7_1:7566670-7566689 None:intergenic 30.0%
! TAATCACAATTGGTGACAAA+TGG + chr7_1:7566993-7567012 Msa1007840:CDS 30.0%
! AATCACAATTGGTGACAAAT+GGG + chr7_1:7566994-7567013 Msa1007840:CDS 30.0%
! GTGATCCTTCTTTTATCAAA+CGG + chr7_1:7567100-7567119 Msa1007840:CDS 30.0%
TCTTTGATAGACATCAAAGA+TGG - chr7_1:7567160-7567179 None:intergenic 30.0%
ATCAACTGCATTTGATGTAA+TGG + chr7_1:7567186-7567205 Msa1007840:CDS 30.0%
TCAACTGCATTTGATGTAAT+GGG + chr7_1:7567187-7567206 Msa1007840:CDS 30.0%
TCTAACAGTCCTTTGTATTT+CGG + chr7_1:7567217-7567236 Msa1007840:CDS 30.0%
AAAAACAACCCTTCTTCTAA+AGG - chr7_1:7567247-7567266 None:intergenic 30.0%
! GTTTTTGATGAAGTGATGAA+GGG + chr7_1:7567304-7567323 Msa1007840:CDS 30.0%
TTATGGTACTAAAGAAAGTG+TGG + chr7_1:7567333-7567352 Msa1007840:CDS 30.0%
! TATGGTACTAAAGAAAGTGT+GGG + chr7_1:7567334-7567353 Msa1007840:CDS 30.0%
! GTGATTTTCGATTCTTTGAT+GGG + chr7_1:7567398-7567417 Msa1007840:CDS 30.0%
ATGAAATTAGGGATGGTTAT+TGG + chr7_1:7567452-7567471 Msa1007840:CDS 30.0%
! AAGTGAGGAGCAATATTTTA+GGG + chr7_1:7567574-7567593 Msa1007840:exon 30.0%
TTCGGCTATATTTGAATTGA+TGG + chr7_1:7567779-7567798 Msa1007840:three_prime_UTR 30.0%
! ATATTTGAATTGATGGCATG+TGG + chr7_1:7567786-7567805 Msa1007840:three_prime_UTR 30.0%
AATAAGCTTGTAGAAGATCT+AGG + chr7_1:7567849-7567868 Msa1007840:three_prime_UTR 30.0%
! AGGATTAGACATTTTGAACT+TGG - chr7_1:7568090-7568109 None:intergenic 30.0%
TTCAAAATGTCTAATCCTGA+AGG + chr7_1:7568092-7568111 Msa1007840:three_prime_UTR 30.0%
! TTTGAAATTGCATCGCATAT+TGG + chr7_1:7568138-7568157 Msa1007840:three_prime_UTR 30.0%
TCTTCATCTCATCCAAAGTT+TGG - chr7_1:7565777-7565796 None:intergenic 35.0%
ATGCTGATATGTTCTCAAGA+TGG + chr7_1:7566246-7566265 Msa1007840:intron 35.0%
GATGATGCTTATTGATCCAA+TGG - chr7_1:7566628-7566647 None:intergenic 35.0%
! ATCTTTTGTGATCAAACCAC+AGG + chr7_1:7566809-7566828 Msa1007840:intron 35.0%
!! GGGTTTTAGTAATTGTTCTC+CGG - chr7_1:7566921-7566940 None:intergenic 35.0%
TTGTCACCAATTGTGATTAG+TGG - chr7_1:7566992-7567011 None:intergenic 35.0%
ATAGCAACAGAAAAGCTTGA+TGG + chr7_1:7567055-7567074 Msa1007840:CDS 35.0%
TAGCAACAGAAAAGCTTGAT+GGG + chr7_1:7567056-7567075 Msa1007840:CDS 35.0%
TCACTGTTCGTTCGAAAATA+TGG - chr7_1:7567084-7567103 None:intergenic 35.0%
TATTGAGTATGATCCCTGAT+GGG - chr7_1:7567135-7567154 None:intergenic 35.0%
CTAACAGTCCTTTGTATTTC+GGG + chr7_1:7567218-7567237 Msa1007840:CDS 35.0%
TAACAGTCCTTTGTATTTCG+GGG + chr7_1:7567219-7567238 Msa1007840:CDS 35.0%
! GGTTTTTGATGAAGTGATGA+AGG + chr7_1:7567303-7567322 Msa1007840:CDS 35.0%
GTGATGAAGGGTTTGTATTA+TGG + chr7_1:7567316-7567335 Msa1007840:CDS 35.0%
! GGTGATTTTCGATTCTTTGA+TGG + chr7_1:7567397-7567416 Msa1007840:CDS 35.0%
! GATTCTTTGATGGGTATTGT+GGG + chr7_1:7567407-7567426 Msa1007840:CDS 35.0%
TTGAGAGATGAAATTAGGGA+TGG + chr7_1:7567445-7567464 Msa1007840:CDS 35.0%
! TAGTCCAAGTGTTGTTGATT+TGG + chr7_1:7567489-7567508 Msa1007840:CDS 35.0%
!! AGTCCAAGTGTTGTTGATTT+GGG + chr7_1:7567490-7567509 Msa1007840:CDS 35.0%
! CAAGTGAGGAGCAATATTTT+AGG + chr7_1:7567573-7567592 Msa1007840:exon 35.0%
! AAATCACGTGTGATTTTTCG+AGG - chr7_1:7567625-7567644 None:intergenic 35.0%
! AAAGTTGGGCTCTAATTGAA+AGG + chr7_1:7567712-7567731 Msa1007840:three_prime_UTR 35.0%
! AAGTTGGGCTCTAATTGAAA+GGG + chr7_1:7567713-7567732 Msa1007840:three_prime_UTR 35.0%
! ACAACTTGTTTGGAAGCTAT+TGG + chr7_1:7567931-7567950 Msa1007840:three_prime_UTR 35.0%
AAATGTCTAATCCTGAAGGA+TGG + chr7_1:7568096-7568115 Msa1007840:three_prime_UTR 35.0%
TGCTTTGACAAATGGCACAT+AGG - chr7_1:7565724-7565743 None:intergenic 40.0%
TGATGAGAGATCAAGTTTGG+TGG - chr7_1:7565871-7565890 None:intergenic 40.0%
AGTCGCTGAAGAAAGGAATT+CGG - chr7_1:7566942-7566961 None:intergenic 40.0%
CATCCACCACTAATCACAAT+TGG + chr7_1:7566983-7567002 Msa1007840:CDS 40.0%
TCACCAATTGTGATTAGTGG+TGG - chr7_1:7566989-7567008 None:intergenic 40.0%
AGCAACAGAAAAGCTTGATG+GGG + chr7_1:7567057-7567076 Msa1007840:CDS 40.0%
TTGGGCCGTTTGATAAAAGA+AGG - chr7_1:7567108-7567127 None:intergenic 40.0%
! CTTTTATCAAACGGCCCAAT+AGG + chr7_1:7567109-7567128 Msa1007840:CDS 40.0%
CTATTGAGTATGATCCCTGA+TGG - chr7_1:7567136-7567155 None:intergenic 40.0%
AACAGTCCTTTGTATTTCGG+GGG + chr7_1:7567220-7567239 Msa1007840:CDS 40.0%
AGTGAGTCCAAAAGATGATG+TGG + chr7_1:7567267-7567286 Msa1007840:CDS 40.0%
AGTCCAAAAGATGATGTGGT+GGG + chr7_1:7567271-7567290 Msa1007840:CDS 40.0%
! TTTCCCACCACATCATCTTT+TGG - chr7_1:7567277-7567296 None:intergenic 40.0%
! ATGATGTGGTGGGAAAAAGT+GGG + chr7_1:7567281-7567300 Msa1007840:CDS 40.0%
TGAAATGGTGAAGAGGAATG+TGG + chr7_1:7567366-7567385 Msa1007840:CDS 40.0%
! CGATTCTTTGATGGGTATTG+TGG + chr7_1:7567406-7567425 Msa1007840:CDS 40.0%
!! GTCCAAGTGTTGTTGATTTG+GGG + chr7_1:7567491-7567510 Msa1007840:CDS 40.0%
TTCCCCAAATCAACAACACT+TGG - chr7_1:7567496-7567515 None:intergenic 40.0%
!! GTTGTTGATTTGGGGAATGT+TGG + chr7_1:7567499-7567518 Msa1007840:CDS 40.0%
TTGGGGAATGTTGGAATTGT+TGG + chr7_1:7567508-7567527 Msa1007840:CDS 40.0%
TGGGGAATGTTGGAATTGTT+GGG + chr7_1:7567509-7567528 Msa1007840:CDS 40.0%
!!! GATTTTTCGAGGAAACGTGA+GGG - chr7_1:7567614-7567633 None:intergenic 40.0%
!!! TGATTTTTCGAGGAAACGTG+AGG - chr7_1:7567615-7567634 None:intergenic 40.0%
!! ATACACATGAAGAGTGCTTG+TGG + chr7_1:7567681-7567700 Msa1007840:three_prime_UTR 40.0%
!! TACACATGAAGAGTGCTTGT+GGG + chr7_1:7567682-7567701 Msa1007840:three_prime_UTR 40.0%
GAGAACATGAGTGTCAATAC+CGG - chr7_1:7567741-7567760 None:intergenic 40.0%
! GCGACTACATCTAGACTTTT+CGG + chr7_1:7567761-7567780 Msa1007840:three_prime_UTR 40.0%
GGCACTAGTATTAACCCTTA+GGG - chr7_1:7567888-7567907 None:intergenic 40.0%
AGTAGATACATCCATCCTTC+AGG - chr7_1:7568110-7568129 None:intergenic 40.0%
AGTTCCTTCACCAAGATCAG+AGG + chr7_1:7565960-7565979 Msa1007840:CDS 45.0%
TAGAGATCCACCTCTGATCT+TGG - chr7_1:7565973-7565992 None:intergenic 45.0%
TCAACGCCGAGACTTATAGA+TGG + chr7_1:7566661-7566680 Msa1007840:intron 45.0%
TGATGACATGTTGCAACCTG+TGG - chr7_1:7566828-7566847 None:intergenic 45.0%
CATGTCATCATCATCACCAC+CGG + chr7_1:7566838-7566857 Msa1007840:CDS 45.0%
!! AGGGTTGGTTTGTCATCATC+CGG - chr7_1:7566860-7566879 None:intergenic 45.0%
AACCAACCCTTAACACCGAT+TGG + chr7_1:7566870-7566889 Msa1007840:CDS 45.0%
!! AGATCGCCAATCGGTGTTAA+GGG - chr7_1:7566879-7566898 None:intergenic 45.0%
!! AAGATCGCCAATCGGTGTTA+AGG - chr7_1:7566880-7566899 None:intergenic 45.0%
ATCTTTCCGTGCAAAACTGG+TGG + chr7_1:7566895-7566914 Msa1007840:CDS 45.0%
GTCGCTGAAGAAAGGAATTC+GGG - chr7_1:7566941-7566960 None:intergenic 45.0%
GGATGATTCACAACAGTGTC+TGG - chr7_1:7566968-7566987 None:intergenic 45.0%
AAAGGTCCCCCGAAATACAA+AGG - chr7_1:7567229-7567248 None:intergenic 45.0%
GAGTCCAAAAGATGATGTGG+TGG + chr7_1:7567270-7567289 Msa1007840:CDS 45.0%
! GATGATGTGGTGGGAAAAAG+TGG + chr7_1:7567280-7567299 Msa1007840:CDS 45.0%
! TGATGTGGTGGGAAAAAGTG+GGG + chr7_1:7567282-7567301 Msa1007840:CDS 45.0%
TTGCTGCTGAAATGGTGAAG+AGG + chr7_1:7567359-7567378 Msa1007840:CDS 45.0%
! TCTTTGATGGGTATTGTGGG+TGG + chr7_1:7567410-7567429 Msa1007840:CDS 45.0%
! CTTTGATGGGTATTGTGGGT+GGG + chr7_1:7567411-7567430 Msa1007840:CDS 45.0%
ATGTTGGAATTGTTGGGCTC+TGG + chr7_1:7567515-7567534 Msa1007840:CDS 45.0%
TGTTGGAATTGTTGGGCTCT+GGG + chr7_1:7567516-7567535 Msa1007840:CDS 45.0%
GGCAAAAGGAAAGTCAAGTG+AGG + chr7_1:7567559-7567578 Msa1007840:CDS 45.0%
AACGTGAGGGTGGGTAAAAT+CGG - chr7_1:7567601-7567620 None:intergenic 45.0%
!! ACACATGAAGAGTGCTTGTG+GGG + chr7_1:7567683-7567702 Msa1007840:three_prime_UTR 45.0%
TTGTGGGGATGGGAAAAGTT+GGG + chr7_1:7567698-7567717 Msa1007840:three_prime_UTR 45.0%
! GGCTCTAATTGAAAGGGTAC+CGG + chr7_1:7567719-7567738 Msa1007840:three_prime_UTR 45.0%
GTGTAGTAGCAAGACCCTAA+GGG + chr7_1:7567871-7567890 Msa1007840:three_prime_UTR 45.0%
GGGCACTAGTATTAACCCTT+AGG - chr7_1:7567889-7567908 None:intergenic 45.0%
TTTGGAAGCTATTGGCAAGC+AGG + chr7_1:7567939-7567958 Msa1007840:three_prime_UTR 45.0%
TCCTTCACCAAGATCAGAGG+TGG + chr7_1:7565963-7565982 Msa1007840:CDS 50.0%
TCCACCTCTGATCTTGGTGA+AGG - chr7_1:7565967-7565986 None:intergenic 50.0%
!! GTTGGTTTGTCATCATCCGG+TGG - chr7_1:7566857-7566876 None:intergenic 50.0%
TGCACGGAAAGATCGCCAAT+CGG - chr7_1:7566888-7566907 None:intergenic 50.0%
GCGATCTTTCCGTGCAAAAC+TGG + chr7_1:7566892-7566911 Msa1007840:CDS 50.0%
CTGGATCAGTCGCTGAAGAA+AGG - chr7_1:7566949-7566968 None:intergenic 50.0%
GTAATCCATGAGCCCGAAAG+AGG + chr7_1:7567022-7567041 Msa1007840:CDS 50.0%
AACAACCTCTTTCGGGCTCA+TGG - chr7_1:7567030-7567049 None:intergenic 50.0%
! TGATCCCTGATGGGCCTATT+GGG - chr7_1:7567126-7567145 None:intergenic 50.0%
! ATGATCCCTGATGGGCCTAT+TGG - chr7_1:7567127-7567146 None:intergenic 50.0%
! TTCGGGGGACCTTTAGAAGA+AGG + chr7_1:7567235-7567254 Msa1007840:CDS 50.0%
! TCGGGGGACCTTTAGAAGAA+GGG + chr7_1:7567236-7567255 Msa1007840:CDS 50.0%
TGTGGGTCTTGCTGCTGAAA+TGG + chr7_1:7567351-7567370 Msa1007840:CDS 50.0%
AAGAGGAATGTGGTCGAGGT+TGG + chr7_1:7567376-7567395 Msa1007840:CDS 50.0%
GACGAAGTTCTCGGGCTTAT+GGG + chr7_1:7567538-7567557 Msa1007840:CDS 50.0%
TTCTCGGGCTTATGGGCAAA+AGG + chr7_1:7567545-7567564 Msa1007840:CDS 50.0%
ACGTGAGGGTGGGTAAAATC+GGG - chr7_1:7567600-7567619 None:intergenic 50.0%
TTTCGAGGAAACGTGAGGGT+GGG - chr7_1:7567610-7567629 None:intergenic 50.0%
!! TTTTCGAGGAAACGTGAGGG+TGG - chr7_1:7567611-7567630 None:intergenic 50.0%
!! ATGAAGAGTGCTTGTGGGGA+TGG + chr7_1:7567687-7567706 Msa1007840:three_prime_UTR 50.0%
!! TGAAGAGTGCTTGTGGGGAT+GGG + chr7_1:7567688-7567707 Msa1007840:three_prime_UTR 50.0%
CTTGTGGGGATGGGAAAAGT+TGG + chr7_1:7567697-7567716 Msa1007840:three_prime_UTR 50.0%
GGTGTAGTAGCAAGACCCTA+AGG + chr7_1:7567870-7567889 Msa1007840:three_prime_UTR 50.0%
!! CGCCAATCGGTGTTAAGGGT+TGG - chr7_1:7566875-7566894 None:intergenic 55.0%
TTCCGTGCAAAACTGGTGGC+CGG + chr7_1:7566899-7566918 Msa1007840:CDS 55.0%
GGTGAAGAGGAATGTGGTCG+AGG + chr7_1:7567372-7567391 Msa1007840:CDS 55.0%
GGACGAAGTTCTCGGGCTTA+TGG + chr7_1:7567537-7567556 Msa1007840:CDS 55.0%
! CTCCGGCCACCAGTTTTGCA+CGG - chr7_1:7566904-7566923 None:intergenic 60.0%
AACGGCCCAATAGGCCCATC+AGG + chr7_1:7567118-7567137 Msa1007840:CDS 60.0%
ACGGCCCAATAGGCCCATCA+GGG + chr7_1:7567119-7567138 Msa1007840:CDS 60.0%
!! GGGCTCTGGGACGAAGTTCT+CGG + chr7_1:7567529-7567548 Msa1007840:CDS 60.0%
!! GGCTCTGGGACGAAGTTCTC+GGG + chr7_1:7567530-7567549 Msa1007840:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 7565713 7568299 7565713 ID=Msa1007840;Name=Msa1007840
chr7_1 mRNA 7565713 7568299 7565713 ID=Msa1007840-mRNA-1;Parent=Msa1007840;Name=Msa1007840-mRNA-1;_AED=0.00;_eAED=0.00;_QI=223|1|1|1|0|0|2|720|291
chr7_1 exon 7565713 7566062 7565713 ID=Msa1007840-mRNA-1:exon:2276;Parent=Msa1007840-mRNA-1
chr7_1 exon 7566831 7568299 7566831 ID=Msa1007840-mRNA-1:exon:2277;Parent=Msa1007840-mRNA-1
chr7_1 five_prime_UTR 7565713 7565935 7565713 ID=Msa1007840-mRNA-1:five_prime_utr;Parent=Msa1007840-mRNA-1
chr7_1 CDS 7565936 7566062 7565936 ID=Msa1007840-mRNA-1:cds;Parent=Msa1007840-mRNA-1
chr7_1 CDS 7566831 7567579 7566831 ID=Msa1007840-mRNA-1:cds;Parent=Msa1007840-mRNA-1
chr7_1 three_prime_UTR 7567580 7568299 7567580 ID=Msa1007840-mRNA-1:three_prime_utr;Parent=Msa1007840-mRNA-1
Gene Sequence

>Msa1007840

ATGGATGCTTGTTTTCTCTCTCACAGTTCCTTCACCAAGATCAGAGGTGGATCTCTACCTTACTCCAAAAGATTTTCTCATCAACAATTCAATTCAAGAAAAGTTGGAGTTCCATTTCATGTATCATGTTGCAACATGTCATCATCATCACCACCGGATGATGACAAACCAACCCTTAACACCGATTGGCGATCTTTCCGTGCAAAACTGGTGGCCGGAGAACAATTACTAAAACCCGAATTCCTTTCTTCAGCGACTGATCCAGACACTGTTGTGAATCATCCACCACTAATCACAATTGGTGACAAATGGGCTCACGTAATCCATGAGCCCGAAAGAGGTTGTTTATTAATAGCAACAGAAAAGCTTGATGGGGTCCATATTTTCGAACGAACAGTGATCCTTCTTTTATCAAACGGCCCAATAGGCCCATCAGGGATCATACTCAATAGACCATCTTTGATGTCTATCAAAGAAACAAGATCAACTGCATTTGATGTAATGGGTACTTTTTCTAACAGTCCTTTGTATTTCGGGGGACCTTTAGAAGAAGGGTTGTTTTTAGTGAGTCCAAAAGATGATGTGGTGGGAAAAAGTGGGGTTTTTGATGAAGTGATGAAGGGTTTGTATTATGGTACTAAAGAAAGTGTGGGTCTTGCTGCTGAAATGGTGAAGAGGAATGTGGTCGAGGTTGGTGATTTTCGATTCTTTGATGGGTATTGTGGGTGGGAGAAAGAACAATTGAGAGATGAAATTAGGGATGGTTATTGGACTGTTGCTGCTTGTAGTCCAAGTGTTGTTGATTTGGGGAATGTTGGAATTGTTGGGCTCTGGGACGAAGTTCTCGGGCTTATGGGCAAAAGGAAAGTCAAGTGA
Protein sequence

>Msa1007840

MDACFLSHSSFTKIRGGSLPYSKRFSHQQFNSRKVGVPFHVSCCNMSSSSPPDDDKPTLNTDWRSFRAKLVAGEQLLKPEFLSSATDPDTVVNHPPLITIGDKWAHVIHEPERGCLLIATEKLDGVHIFERTVILLLSNGPIGPSGIILNRPSLMSIKETRSTAFDVMGTFSNSPLYFGGPLEEGLFLVSPKDDVVGKSGVFDEVMKGLYYGTKESVGLAAEMVKRNVVEVGDFRFFDGYCGWEKEQLRDEIRDGYWTVAACSPSVVDLGNVGIVGLWDEVLGLMGKRKVK*