AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1016080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1016080 MtrunA17_Chr7g0230041 84.507 142 1 1 1 121 1 142 7.83e-52 176
Msa1016080 MtrunA17_Chr3g0116311 43.137 102 38 2 15 96 49 150 5.84e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1016080 AT3G27260.3 36.441 118 53 2 1 96 2 119 2.62e-12 63.9
Msa1016080 AT3G27260.4 36.441 118 53 2 1 96 2 119 2.94e-12 63.9
Msa1016080 AT3G27260.1 36.441 118 53 2 1 96 2 119 3.00e-12 63.9

Find 65 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAATCTTCCCAGAAAATT+TGG 0.151619 7_1:-31660169 None:intergenic
GGTGGAGGCGGAGGAAATAA+TGG 0.356155 7_1:+31660087 Msa1016080:CDS
CAAACTGATGGACTTCTTTG+AGG 0.362140 7_1:-31659845 None:intergenic
GAAATCCAGTGGGAGATTCT+AGG 0.370098 7_1:+31660109 Msa1016080:CDS
ATAAGGCTCGCGGATGGAAT+CGG 0.370330 7_1:+31660190 Msa1016080:CDS
GGATGGAATCGGGGCACTTC+TGG 0.370747 7_1:+31660201 Msa1016080:CDS
TAAGGCTCGCGGATGGAATC+GGG 0.393944 7_1:+31660191 Msa1016080:CDS
AGGAAGGGATGGAAAACAAT+AGG 0.405271 7_1:+31659725 Msa1016080:intron
AGGGTTGGTGGAGGTTATTT+CGG 0.411615 7_1:+31659745 Msa1016080:CDS
TACAAGATCAACTTGGTTGT+AGG 0.413212 7_1:+31659465 Msa1016080:five_prime_UTR
TTCTTCTTCGTTGCTGAGAT+CGG 0.421724 7_1:+31659518 Msa1016080:five_prime_UTR
TTCTGGAAAGTTTGAGGCTC+CGG 0.424913 7_1:+31660218 Msa1016080:CDS
TGAGGGGCCAGTGGAGTCTT+CGG 0.443601 7_1:-31659827 None:intergenic
GACCCCTCAGATTCACCAAC+TGG 0.447936 7_1:-31659772 None:intergenic
ATTTCAACTAGCTGCAGTAC+AGG 0.452108 7_1:+31659608 Msa1016080:five_prime_UTR
GGTGGAGGAGGTGGAGGCGG+AGG 0.458633 7_1:+31660078 Msa1016080:CDS
AGCGGTGGAGGAGGTGGAGG+CGG 0.460958 7_1:+31660075 Msa1016080:CDS
AAACTGATGGACTTCTTTGA+GGG 0.464294 7_1:-31659844 None:intergenic
TGTAGCGGTGGAGGAGGTGG+AGG 0.467358 7_1:+31660072 Msa1016080:CDS
GGGCACTTCTGGAAAGTTTG+AGG 0.469177 7_1:+31660212 Msa1016080:CDS
AGTTGTAGCGGTGGAGGAGG+TGG 0.472540 7_1:+31660069 Msa1016080:CDS
GCCGTGCGATGTATAATAAA+CGG 0.475668 7_1:-31659390 None:intergenic
AACAAAGAATTTCCCATGGC+AGG 0.481787 7_1:-31660255 None:intergenic
GGTGAATCTGAGGGGTCATC+GGG 0.481894 7_1:+31659778 Msa1016080:CDS
ACAGACTCGTTCGCCTGCCA+TGG 0.488229 7_1:+31660242 Msa1016080:CDS
ATTTAATGAGCAGGAAGGGA+TGG 0.499991 7_1:+31659714 Msa1016080:intron
TGGTGAATCTGAGGGGTCAT+CGG 0.507104 7_1:+31659777 Msa1016080:CDS
GGAAGGGATGGAAAACAATA+GGG 0.508364 7_1:+31659726 Msa1016080:intron
CAAACGCACCGCGGCGATGA+CGG 0.515758 7_1:-31659882 None:intergenic
GGACTTCTTTGAGGGGCCAG+TGG 0.524882 7_1:-31659836 None:intergenic
GCGAATTGAATTCAAACTGA+TGG 0.526109 7_1:-31659857 None:intergenic
ATTCTAAGTTGTAGCGGTGG+AGG 0.528667 7_1:+31660063 Msa1016080:CDS
ATGGAAAACAATAGGGTTGG+TGG 0.530884 7_1:+31659733 Msa1016080:CDS
ACGACAAAAGCAGCTTAAGC+GGG 0.534526 7_1:-31659545 None:intergenic
TTCTTCTTACAAGATCAACT+TGG 0.536200 7_1:+31659458 Msa1016080:five_prime_UTR
TGATGACAATGTAACACCAT+TGG 0.550544 7_1:-31660031 None:intergenic
AGGAAATAATGGAAATCCAG+TGG 0.554687 7_1:+31660098 Msa1016080:CDS
GCAGGCGAACGAGTCTGTAC+CGG 0.555766 7_1:-31660237 None:intergenic
ACTGTTTCCGAAGACTCCAC+TGG 0.556075 7_1:+31659820 Msa1016080:CDS
CTAAGTTGTAGCGGTGGAGG+AGG 0.558478 7_1:+31660066 Msa1016080:CDS
TGCAAATTGCAGATGAAAGT+CGG 0.559720 7_1:+31659422 Msa1016080:five_prime_UTR
TCCGTGTCTAGTTCTTTACC+TGG 0.561536 7_1:+31660138 Msa1016080:CDS
CAGACTCGTTCGCCTGCCAT+GGG 0.567601 7_1:+31660243 Msa1016080:CDS
ATTGAACTGCAGAGATCCAA+TGG 0.575028 7_1:+31660015 Msa1016080:CDS
TAATCCAGTTGGTGAATCTG+AGG 0.575122 7_1:+31659768 Msa1016080:CDS
GGTTATTTCGGTAATCCAGT+TGG 0.578779 7_1:+31659757 Msa1016080:CDS
AACGACAAAAGCAGCTTAAG+CGG 0.581094 7_1:-31659546 None:intergenic
TCCGTTTATTATACATCGCA+CGG 0.585020 7_1:+31659389 None:intergenic
CATCAACAAAGAATTTCCCA+TGG 0.586117 7_1:-31660259 None:intergenic
AATCCAGTTGGTGAATCTGA+GGG 0.593793 7_1:+31659769 Msa1016080:CDS
AGATTAATAAGGCTCGCGGA+TGG 0.596115 7_1:+31660184 Msa1016080:CDS
AAGTACAAGGTCTGAAGCAA+CGG 0.597067 7_1:+31659950 Msa1016080:intron
GAAAACAATAGGGTTGGTGG+AGG 0.613219 7_1:+31659736 Msa1016080:CDS
AACTGATGGACTTCTTTGAG+GGG 0.614822 7_1:-31659843 None:intergenic
GGGATGGAAAACAATAGGGT+TGG 0.616224 7_1:+31659730 Msa1016080:exon
TTTCAACTAGCTGCAGTACA+GGG 0.625670 7_1:+31659609 Msa1016080:five_prime_UTR
ACGCACCGCGGCGATGACGG+TGG 0.626217 7_1:-31659879 None:intergenic
GATATTCTAAGTTGTAGCGG+TGG 0.633059 7_1:+31660060 Msa1016080:CDS
GGAAATAATGGAAATCCAGT+GGG 0.637088 7_1:+31660099 Msa1016080:CDS
AAGGCTCGCGGATGGAATCG+GGG 0.650634 7_1:+31660192 Msa1016080:CDS
AGTGATATTCTAAGTTGTAG+CGG 0.659543 7_1:+31660057 Msa1016080:CDS
GGGAAGATTAATAAGGCTCG+CGG 0.666256 7_1:+31660180 Msa1016080:CDS
ATTCGCCACCGTCATCGCCG+CGG 0.677129 7_1:+31659874 Msa1016080:CDS
ACCAGGTAAAGAACTAGACA+CGG 0.682305 7_1:-31660139 None:intergenic
ATCCAGTTGGTGAATCTGAG+GGG 0.685743 7_1:+31659770 Msa1016080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAACTGCTAAAATTTAA+TGG - chr7_1:31659680-31659699 None:intergenic 15.0%
!!! TTTAATTTAATTTAATGAGC+AGG + chr7_1:31659705-31659724 Msa1016080:intron 15.0%
!! TTAAAACTGCTAAAATTTAA+TGG - chr7_1:31659680-31659699 None:intergenic 15.0%
!!! TTTAATTTAATTTAATGAGC+AGG + chr7_1:31659705-31659724 Msa1016080:intron 15.0%
!!! ATAGGTGATAATTTTGATAA+TGG - chr7_1:31659928-31659947 None:intergenic 20.0%
!!! ATAGGTGATAATTTTGATAA+TGG - chr7_1:31659928-31659947 None:intergenic 20.0%
!! ATTTAATTTAATGAGCAGGA+AGG + chr7_1:31659709-31659728 Msa1016080:intron 25.0%
!! TTTAATTTAATGAGCAGGAA+GGG + chr7_1:31659710-31659729 Msa1016080:intron 25.0%
!!! GATAATGGAAGAATTTGTAT+AGG - chr7_1:31659913-31659932 None:intergenic 25.0%
!! GTAGTAAATCCAAATTTTCT+GGG + chr7_1:31660160-31660179 Msa1016080:intron 25.0%
! ATTAATCTTCCCAGAAAATT+TGG - chr7_1:31660172-31660191 None:intergenic 25.0%
!!! ATTTTCTGGGAAGATTAATA+AGG + chr7_1:31660173-31660192 Msa1016080:CDS 25.0%
!! ATTTAATTTAATGAGCAGGA+AGG + chr7_1:31659709-31659728 Msa1016080:intron 25.0%
!! TTTAATTTAATGAGCAGGAA+GGG + chr7_1:31659710-31659729 Msa1016080:intron 25.0%
!!! GATAATGGAAGAATTTGTAT+AGG - chr7_1:31659913-31659932 None:intergenic 25.0%
!! GTAGTAAATCCAAATTTTCT+GGG + chr7_1:31660160-31660179 Msa1016080:intron 25.0%
! ATTAATCTTCCCAGAAAATT+TGG - chr7_1:31660172-31660191 None:intergenic 25.0%
!!! ATTTTCTGGGAAGATTAATA+AGG + chr7_1:31660173-31660192 Msa1016080:CDS 25.0%
TTCTTCTTACAAGATCAACT+TGG + chr7_1:31659458-31659477 Msa1016080:five_prime_UTR 30.0%
AAGAAGAAGAAGAAGAAAGT+TGG - chr7_1:31659494-31659513 None:intergenic 30.0%
TTATCACCTATTGAAGTACA+AGG + chr7_1:31659937-31659956 Msa1016080:intron 30.0%
AGTGATATTCTAAGTTGTAG+CGG + chr7_1:31660057-31660076 Msa1016080:CDS 30.0%
GAAAATTTGGATTTACTACC+AGG - chr7_1:31660159-31660178 None:intergenic 30.0%
! GGTAGTAAATCCAAATTTTC+TGG + chr7_1:31660159-31660178 Msa1016080:intron 30.0%
TTCTTCTTACAAGATCAACT+TGG + chr7_1:31659458-31659477 Msa1016080:five_prime_UTR 30.0%
AAGAAGAAGAAGAAGAAAGT+TGG - chr7_1:31659494-31659513 None:intergenic 30.0%
TTATCACCTATTGAAGTACA+AGG + chr7_1:31659937-31659956 Msa1016080:intron 30.0%
AGTGATATTCTAAGTTGTAG+CGG + chr7_1:31660057-31660076 Msa1016080:CDS 30.0%
GAAAATTTGGATTTACTACC+AGG - chr7_1:31660159-31660178 None:intergenic 30.0%
! GGTAGTAAATCCAAATTTTC+TGG + chr7_1:31660159-31660178 Msa1016080:intron 30.0%
TGCAAATTGCAGATGAAAGT+CGG + chr7_1:31659422-31659441 Msa1016080:five_prime_UTR 35.0%
TACAAGATCAACTTGGTTGT+AGG + chr7_1:31659465-31659484 Msa1016080:five_prime_UTR 35.0%
!! AAACTGATGGACTTCTTTGA+GGG - chr7_1:31659847-31659866 None:intergenic 35.0%
GCGAATTGAATTCAAACTGA+TGG - chr7_1:31659860-31659879 None:intergenic 35.0%
TTCAGACCTTGTACTTCAAT+AGG - chr7_1:31659946-31659965 None:intergenic 35.0%
TGATGACAATGTAACACCAT+TGG - chr7_1:31660034-31660053 None:intergenic 35.0%
! AGGAAATAATGGAAATCCAG+TGG + chr7_1:31660098-31660117 Msa1016080:CDS 35.0%
! GGAAATAATGGAAATCCAGT+GGG + chr7_1:31660099-31660118 Msa1016080:CDS 35.0%
TGCAAATTGCAGATGAAAGT+CGG + chr7_1:31659422-31659441 Msa1016080:five_prime_UTR 35.0%
TACAAGATCAACTTGGTTGT+AGG + chr7_1:31659465-31659484 Msa1016080:five_prime_UTR 35.0%
!! AAACTGATGGACTTCTTTGA+GGG - chr7_1:31659847-31659866 None:intergenic 35.0%
GCGAATTGAATTCAAACTGA+TGG - chr7_1:31659860-31659879 None:intergenic 35.0%
TTCAGACCTTGTACTTCAAT+AGG - chr7_1:31659946-31659965 None:intergenic 35.0%
TGATGACAATGTAACACCAT+TGG - chr7_1:31660034-31660053 None:intergenic 35.0%
! AGGAAATAATGGAAATCCAG+TGG + chr7_1:31660098-31660117 Msa1016080:CDS 35.0%
! GGAAATAATGGAAATCCAGT+GGG + chr7_1:31660099-31660118 Msa1016080:CDS 35.0%
TTCTTCTTCGTTGCTGAGAT+CGG + chr7_1:31659518-31659537 Msa1016080:five_prime_UTR 40.0%
AACGACAAAAGCAGCTTAAG+CGG - chr7_1:31659549-31659568 None:intergenic 40.0%
!! TTTTGTCGTTTGCTGAGCTA+GGG + chr7_1:31659559-31659578 Msa1016080:five_prime_UTR 40.0%
ATTTCAACTAGCTGCAGTAC+AGG + chr7_1:31659608-31659627 Msa1016080:five_prime_UTR 40.0%
TTTCAACTAGCTGCAGTACA+GGG + chr7_1:31659609-31659628 Msa1016080:five_prime_UTR 40.0%
ATTTAATGAGCAGGAAGGGA+TGG + chr7_1:31659714-31659733 Msa1016080:intron 40.0%
AGGAAGGGATGGAAAACAAT+AGG + chr7_1:31659725-31659744 Msa1016080:intron 40.0%
GGAAGGGATGGAAAACAATA+GGG + chr7_1:31659726-31659745 Msa1016080:intron 40.0%
ATGGAAAACAATAGGGTTGG+TGG + chr7_1:31659733-31659752 Msa1016080:CDS 40.0%
! GGTTATTTCGGTAATCCAGT+TGG + chr7_1:31659757-31659776 Msa1016080:CDS 40.0%
!! TAATCCAGTTGGTGAATCTG+AGG + chr7_1:31659768-31659787 Msa1016080:CDS 40.0%
!! AATCCAGTTGGTGAATCTGA+GGG + chr7_1:31659769-31659788 Msa1016080:CDS 40.0%
!! AACTGATGGACTTCTTTGAG+GGG - chr7_1:31659846-31659865 None:intergenic 40.0%
!! CAAACTGATGGACTTCTTTG+AGG - chr7_1:31659848-31659867 None:intergenic 40.0%
! AAGTACAAGGTCTGAAGCAA+CGG + chr7_1:31659950-31659969 Msa1016080:intron 40.0%
ATTGAACTGCAGAGATCCAA+TGG + chr7_1:31660015-31660034 Msa1016080:CDS 40.0%
GATATTCTAAGTTGTAGCGG+TGG + chr7_1:31660060-31660079 Msa1016080:CDS 40.0%
! TTTTTCCTAGAATCTCCCAC+TGG - chr7_1:31660117-31660136 None:intergenic 40.0%
ACCAGGTAAAGAACTAGACA+CGG - chr7_1:31660142-31660161 None:intergenic 40.0%
AACAAAGAATTTCCCATGGC+AGG - chr7_1:31660258-31660277 None:intergenic 40.0%
TTCTTCTTCGTTGCTGAGAT+CGG + chr7_1:31659518-31659537 Msa1016080:five_prime_UTR 40.0%
AACGACAAAAGCAGCTTAAG+CGG - chr7_1:31659549-31659568 None:intergenic 40.0%
!! TTTTGTCGTTTGCTGAGCTA+GGG + chr7_1:31659559-31659578 Msa1016080:five_prime_UTR 40.0%
ATTTCAACTAGCTGCAGTAC+AGG + chr7_1:31659608-31659627 Msa1016080:five_prime_UTR 40.0%
TTTCAACTAGCTGCAGTACA+GGG + chr7_1:31659609-31659628 Msa1016080:five_prime_UTR 40.0%
ATTTAATGAGCAGGAAGGGA+TGG + chr7_1:31659714-31659733 Msa1016080:intron 40.0%
AGGAAGGGATGGAAAACAAT+AGG + chr7_1:31659725-31659744 Msa1016080:intron 40.0%
GGAAGGGATGGAAAACAATA+GGG + chr7_1:31659726-31659745 Msa1016080:intron 40.0%
ATGGAAAACAATAGGGTTGG+TGG + chr7_1:31659733-31659752 Msa1016080:CDS 40.0%
! GGTTATTTCGGTAATCCAGT+TGG + chr7_1:31659757-31659776 Msa1016080:CDS 40.0%
!! TAATCCAGTTGGTGAATCTG+AGG + chr7_1:31659768-31659787 Msa1016080:CDS 40.0%
!! AATCCAGTTGGTGAATCTGA+GGG + chr7_1:31659769-31659788 Msa1016080:CDS 40.0%
!! AACTGATGGACTTCTTTGAG+GGG - chr7_1:31659846-31659865 None:intergenic 40.0%
!! CAAACTGATGGACTTCTTTG+AGG - chr7_1:31659848-31659867 None:intergenic 40.0%
! AAGTACAAGGTCTGAAGCAA+CGG + chr7_1:31659950-31659969 Msa1016080:intron 40.0%
ATTGAACTGCAGAGATCCAA+TGG + chr7_1:31660015-31660034 Msa1016080:CDS 40.0%
GATATTCTAAGTTGTAGCGG+TGG + chr7_1:31660060-31660079 Msa1016080:CDS 40.0%
! TTTTTCCTAGAATCTCCCAC+TGG - chr7_1:31660117-31660136 None:intergenic 40.0%
ACCAGGTAAAGAACTAGACA+CGG - chr7_1:31660142-31660161 None:intergenic 40.0%
AACAAAGAATTTCCCATGGC+AGG - chr7_1:31660258-31660277 None:intergenic 40.0%
ACGACAAAAGCAGCTTAAGC+GGG - chr7_1:31659548-31659567 None:intergenic 45.0%
!! CTTTTGTCGTTTGCTGAGCT+AGG + chr7_1:31659558-31659577 Msa1016080:five_prime_UTR 45.0%
GGGATGGAAAACAATAGGGT+TGG + chr7_1:31659730-31659749 Msa1016080:exon 45.0%
!! GAAAACAATAGGGTTGGTGG+AGG + chr7_1:31659736-31659755 Msa1016080:CDS 45.0%
!! AGGGTTGGTGGAGGTTATTT+CGG + chr7_1:31659745-31659764 Msa1016080:CDS 45.0%
!! ATCCAGTTGGTGAATCTGAG+GGG + chr7_1:31659770-31659789 Msa1016080:CDS 45.0%
ATTCTAAGTTGTAGCGGTGG+AGG + chr7_1:31660063-31660082 Msa1016080:CDS 45.0%
GAAATCCAGTGGGAGATTCT+AGG + chr7_1:31660109-31660128 Msa1016080:CDS 45.0%
!! TCCGTGTCTAGTTCTTTACC+TGG + chr7_1:31660138-31660157 Msa1016080:CDS 45.0%
GGGAAGATTAATAAGGCTCG+CGG + chr7_1:31660180-31660199 Msa1016080:CDS 45.0%
AGATTAATAAGGCTCGCGGA+TGG + chr7_1:31660184-31660203 Msa1016080:CDS 45.0%
! TTCTGGAAAGTTTGAGGCTC+CGG + chr7_1:31660218-31660237 Msa1016080:CDS 45.0%
ACGACAAAAGCAGCTTAAGC+GGG - chr7_1:31659548-31659567 None:intergenic 45.0%
!! CTTTTGTCGTTTGCTGAGCT+AGG + chr7_1:31659558-31659577 Msa1016080:five_prime_UTR 45.0%
GGGATGGAAAACAATAGGGT+TGG + chr7_1:31659730-31659749 Msa1016080:exon 45.0%
!! GAAAACAATAGGGTTGGTGG+AGG + chr7_1:31659736-31659755 Msa1016080:CDS 45.0%
!! AGGGTTGGTGGAGGTTATTT+CGG + chr7_1:31659745-31659764 Msa1016080:CDS 45.0%
!! ATCCAGTTGGTGAATCTGAG+GGG + chr7_1:31659770-31659789 Msa1016080:CDS 45.0%
ATTCTAAGTTGTAGCGGTGG+AGG + chr7_1:31660063-31660082 Msa1016080:CDS 45.0%
GAAATCCAGTGGGAGATTCT+AGG + chr7_1:31660109-31660128 Msa1016080:CDS 45.0%
!! TCCGTGTCTAGTTCTTTACC+TGG + chr7_1:31660138-31660157 Msa1016080:CDS 45.0%
GGGAAGATTAATAAGGCTCG+CGG + chr7_1:31660180-31660199 Msa1016080:CDS 45.0%
AGATTAATAAGGCTCGCGGA+TGG + chr7_1:31660184-31660203 Msa1016080:CDS 45.0%
! TTCTGGAAAGTTTGAGGCTC+CGG + chr7_1:31660218-31660237 Msa1016080:CDS 45.0%
TGGTGAATCTGAGGGGTCAT+CGG + chr7_1:31659777-31659796 Msa1016080:CDS 50.0%
ACTGTTTCCGAAGACTCCAC+TGG + chr7_1:31659820-31659839 Msa1016080:CDS 50.0%
ATAAGGCTCGCGGATGGAAT+CGG + chr7_1:31660190-31660209 Msa1016080:CDS 50.0%
! GGGCACTTCTGGAAAGTTTG+AGG + chr7_1:31660212-31660231 Msa1016080:CDS 50.0%
TGGTGAATCTGAGGGGTCAT+CGG + chr7_1:31659777-31659796 Msa1016080:CDS 50.0%
ACTGTTTCCGAAGACTCCAC+TGG + chr7_1:31659820-31659839 Msa1016080:CDS 50.0%
ATAAGGCTCGCGGATGGAAT+CGG + chr7_1:31660190-31660209 Msa1016080:CDS 50.0%
! GGGCACTTCTGGAAAGTTTG+AGG + chr7_1:31660212-31660231 Msa1016080:CDS 50.0%
GACCCCTCAGATTCACCAAC+TGG - chr7_1:31659775-31659794 None:intergenic 55.0%
GGTGAATCTGAGGGGTCATC+GGG + chr7_1:31659778-31659797 Msa1016080:CDS 55.0%
CTAAGTTGTAGCGGTGGAGG+AGG + chr7_1:31660066-31660085 Msa1016080:CDS 55.0%
GGTGGAGGCGGAGGAAATAA+TGG + chr7_1:31660087-31660106 Msa1016080:CDS 55.0%
TAAGGCTCGCGGATGGAATC+GGG + chr7_1:31660191-31660210 Msa1016080:CDS 55.0%
GACCCCTCAGATTCACCAAC+TGG - chr7_1:31659775-31659794 None:intergenic 55.0%
GGTGAATCTGAGGGGTCATC+GGG + chr7_1:31659778-31659797 Msa1016080:CDS 55.0%
CTAAGTTGTAGCGGTGGAGG+AGG + chr7_1:31660066-31660085 Msa1016080:CDS 55.0%
GGTGGAGGCGGAGGAAATAA+TGG + chr7_1:31660087-31660106 Msa1016080:CDS 55.0%
TAAGGCTCGCGGATGGAATC+GGG + chr7_1:31660191-31660210 Msa1016080:CDS 55.0%
TGAGGGGCCAGTGGAGTCTT+CGG - chr7_1:31659830-31659849 None:intergenic 60.0%
! GGACTTCTTTGAGGGGCCAG+TGG - chr7_1:31659839-31659858 None:intergenic 60.0%
!! TAGGAGCACCAAACGCACCG+CGG - chr7_1:31659894-31659913 None:intergenic 60.0%
AGTTGTAGCGGTGGAGGAGG+TGG + chr7_1:31660069-31660088 Msa1016080:CDS 60.0%
AAGGCTCGCGGATGGAATCG+GGG + chr7_1:31660192-31660211 Msa1016080:CDS 60.0%
! GGATGGAATCGGGGCACTTC+TGG + chr7_1:31660201-31660220 Msa1016080:CDS 60.0%
GCAGGCGAACGAGTCTGTAC+CGG - chr7_1:31660240-31660259 None:intergenic 60.0%
ACAGACTCGTTCGCCTGCCA+TGG + chr7_1:31660242-31660261 Msa1016080:CDS 60.0%
CAGACTCGTTCGCCTGCCAT+GGG + chr7_1:31660243-31660262 Msa1016080:CDS 60.0%
TGAGGGGCCAGTGGAGTCTT+CGG - chr7_1:31659830-31659849 None:intergenic 60.0%
! GGACTTCTTTGAGGGGCCAG+TGG - chr7_1:31659839-31659858 None:intergenic 60.0%
!! TAGGAGCACCAAACGCACCG+CGG - chr7_1:31659894-31659913 None:intergenic 60.0%
AGTTGTAGCGGTGGAGGAGG+TGG + chr7_1:31660069-31660088 Msa1016080:CDS 60.0%
AAGGCTCGCGGATGGAATCG+GGG + chr7_1:31660192-31660211 Msa1016080:CDS 60.0%
! GGATGGAATCGGGGCACTTC+TGG + chr7_1:31660201-31660220 Msa1016080:CDS 60.0%
GCAGGCGAACGAGTCTGTAC+CGG - chr7_1:31660240-31660259 None:intergenic 60.0%
ACAGACTCGTTCGCCTGCCA+TGG + chr7_1:31660242-31660261 Msa1016080:CDS 60.0%
CAGACTCGTTCGCCTGCCAT+GGG + chr7_1:31660243-31660262 Msa1016080:CDS 60.0%
ATTCGCCACCGTCATCGCCG+CGG + chr7_1:31659874-31659893 Msa1016080:CDS 65.0%
CAAACGCACCGCGGCGATGA+CGG - chr7_1:31659885-31659904 None:intergenic 65.0%
TGTAGCGGTGGAGGAGGTGG+AGG + chr7_1:31660072-31660091 Msa1016080:CDS 65.0%
ATTCGCCACCGTCATCGCCG+CGG + chr7_1:31659874-31659893 Msa1016080:CDS 65.0%
CAAACGCACCGCGGCGATGA+CGG - chr7_1:31659885-31659904 None:intergenic 65.0%
TGTAGCGGTGGAGGAGGTGG+AGG + chr7_1:31660072-31660091 Msa1016080:CDS 65.0%
CGTCATCGCCGCGGTGCGTT+TGG + chr7_1:31659883-31659902 Msa1016080:intron 70.0%
AGCGGTGGAGGAGGTGGAGG+CGG + chr7_1:31660075-31660094 Msa1016080:CDS 70.0%
CGTCATCGCCGCGGTGCGTT+TGG + chr7_1:31659883-31659902 Msa1016080:intron 70.0%
AGCGGTGGAGGAGGTGGAGG+CGG + chr7_1:31660075-31660094 Msa1016080:CDS 70.0%
! ACGCACCGCGGCGATGACGG+TGG - chr7_1:31659882-31659901 None:intergenic 75.0%
GGTGGAGGAGGTGGAGGCGG+AGG + chr7_1:31660078-31660097 Msa1016080:CDS 75.0%
! ACGCACCGCGGCGATGACGG+TGG - chr7_1:31659882-31659901 None:intergenic 75.0%
GGTGGAGGAGGTGGAGGCGG+AGG + chr7_1:31660078-31660097 Msa1016080:CDS 75.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 31659392 31660279 31659392 ID=Msa1016080;Name=Msa1016080
chr7_1 mRNA 31659392 31660279 31659392 ID=Msa1016080-mRNA-1;Parent=Msa1016080;Name=Msa1016080-mRNA-1;_AED=0.45;_eAED=0.45;_QI=245|0.33|0.25|1|0.33|0.25|4|0|159
chr7_1 exon 31659392 31659630 31659392 ID=Msa1016080-mRNA-1:exon:6281;Parent=Msa1016080-mRNA-1
chr7_1 exon 31659727 31659895 31659727 ID=Msa1016080-mRNA-1:exon:6282;Parent=Msa1016080-mRNA-1
chr7_1 exon 31659959 31660159 31659959 ID=Msa1016080-mRNA-1:exon:6283;Parent=Msa1016080-mRNA-1
chr7_1 exon 31660164 31660279 31660164 ID=Msa1016080-mRNA-1:exon:6284;Parent=Msa1016080-mRNA-1
chr7_1 five_prime_UTR 31659392 31659630 31659392 ID=Msa1016080-mRNA-1:five_prime_utr;Parent=Msa1016080-mRNA-1
chr7_1 five_prime_UTR 31659727 31659732 31659727 ID=Msa1016080-mRNA-1:five_prime_utr;Parent=Msa1016080-mRNA-1
chr7_1 CDS 31659733 31659895 31659733 ID=Msa1016080-mRNA-1:cds;Parent=Msa1016080-mRNA-1
chr7_1 CDS 31659959 31660159 31659959 ID=Msa1016080-mRNA-1:cds;Parent=Msa1016080-mRNA-1
chr7_1 CDS 31660164 31660279 31660164 ID=Msa1016080-mRNA-1:cds;Parent=Msa1016080-mRNA-1
Gene Sequence

>Msa1016080

ATGGAAAACAATAGGGTTGGTGGAGGTTATTTCGGTAATCCAGTTGGTGAATCTGAGGGGTCATCGGGACAAATAGACACTGAAATTACTGTTTCCGAAGACTCCACTGGCCCCTCAAAGAAGTCCATCAGTTTGAATTCAATTCGCCACCGTCATCGCCGCGGTCTGAAGCAACGGTTGAGATCTGAGATTGAACAGATTCGTCAACTTCAGAAGAGAATTGAACTGCAGAGATCCAATGGTGTTACATTGTCATCATCTAGTGATATTCTAAGTTGTAGCGGTGGAGGAGGTGGAGGCGGAGGAAATAATGGAAATCCAGTGGGAGATTCTAGGAAAAAATCCGTGTCTAGTTCTTTACCTGTAAATCCAAATTTTCTGGGAAGATTAATAAGGCTCGCGGATGGAATCGGGGCACTTCTGGAAAGTTTGAGGCTCCGGTACAGACTCGTTCGCCTGCCATGGGAAATTCTTTGTTGA
Protein sequence

>Msa1016080

MENNRVGGGYFGNPVGESEGSSGQIDTEITVSEDSTGPSKKSISLNSIRHRHRRGLKQRLRSEIEQIRQLQKRIELQRSNGVTLSSSSDILSCSGGGGGGGGNNGNPVGDSRKKSVSSSLPVNPNFLGRLIRLADGIGALLESLRLRYRLVRLPWEILC*