AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1021250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1021250 MtrunA17_Chr7g0237441 97.759 580 13 0 1 580 1 580 0.0 1178
Msa1021250 MtrunA17_Chr7g0237491 88.621 580 66 0 1 580 1 580 0.0 1071
Msa1021250 MtrunA17_Chr7g0238351 86.747 581 76 1 1 580 1 581 0.0 1051
Msa1021250 MtrunA17_Chr7g0238941 70.890 584 160 5 1 580 1 578 0.0 867
Msa1021250 MtrunA17_Chr4g0031901 70.486 576 155 6 1 568 5 573 0.0 842
Msa1021250 MtrunA17_Chr7g0238981 69.257 592 164 4 6 580 3 593 0.0 836
Msa1021250 MtrunA17_Chr4g0005921 57.371 563 226 4 18 580 9 557 0.0 663
Msa1021250 MtrunA17_Chr5g0429511 54.799 573 240 8 9 580 4 558 0.0 657
Msa1021250 MtrunA17_Chr3g0104061 57.401 554 219 6 27 580 20 556 0.0 655
Msa1021250 MtrunA17_Chr5g0404341 56.162 568 225 9 15 580 21 566 0.0 650
Msa1021250 MtrunA17_Chr5g0404361 54.545 572 240 8 11 580 58 611 0.0 643
Msa1021250 MtrunA17_Chr5g0436461 52.373 569 249 9 15 580 5 554 0.0 606
Msa1021250 MtrunA17_Chr5g0437681 49.826 574 270 10 13 580 6 567 0.0 573
Msa1021250 MtrunA17_Chr5g0431581 50.086 579 263 10 9 580 1 560 0.0 557
Msa1021250 MtrunA17_Chr3g0114211 47.521 585 282 11 4 580 3 570 0.0 555
Msa1021250 MtrunA17_Chr2g0278811 44.992 609 293 9 5 580 8 607 0.0 529
Msa1021250 MtrunA17_Chr7g0240971 48.288 584 276 10 9 580 1 570 4.96e-179 518
Msa1021250 MtrunA17_Chr8g0346581 45.730 562 279 10 27 580 28 571 7.08e-174 504
Msa1021250 MtrunA17_Chr7g0240991 43.783 571 300 11 19 580 13 571 6.85e-170 494
Msa1021250 MtrunA17_Chr4g0008521 45.552 562 275 8 34 580 27 572 4.09e-165 482
Msa1021250 MtrunA17_Chr3g0133861 45.245 347 162 9 235 570 1 330 1.73e-87 275
Msa1021250 MtrunA17_Chr8g0382691 32.465 576 316 21 24 566 26 561 5.39e-74 246
Msa1021250 MtrunA17_Chr3g0118321 32.112 573 325 16 11 554 4 541 1.20e-69 234
Msa1021250 MtrunA17_Chr3g0118331 31.283 569 334 17 11 554 4 540 4.33e-66 225
Msa1021250 MtrunA17_Chr3g0133871 45.098 204 106 2 14 212 9 211 1.75e-60 200
Msa1021250 MtrunA17_Chr5g0436431 45.798 238 111 6 303 538 7 228 8.66e-60 198
Msa1021250 MtrunA17_Chr4g0056511 28.423 577 370 18 14 580 6 549 4.21e-50 182
Msa1021250 MtrunA17_Chr3g0079321 25.865 549 359 17 9 545 4 516 1.79e-45 169
Msa1021250 MtrunA17_Chr1g0157341 25.823 577 367 16 17 580 4 532 4.17e-43 162
Msa1021250 MtrunA17_Chr4g0038671 25.800 531 341 14 15 540 10 492 4.15e-41 156
Msa1021250 MtrunA17_Chr3g0143671 24.776 557 357 15 33 580 30 533 2.13e-40 154
Msa1021250 MtrunA17_Chr4g0024861 26.040 553 355 18 33 580 27 530 1.51e-38 149
Msa1021250 MtrunA17_Chr7g0234061 26.729 535 335 21 34 554 30 521 4.51e-37 145
Msa1021250 MtrunA17_Chr3g0121171 24.863 547 355 13 23 556 1159 1662 3.02e-35 142
Msa1021250 MtrunA17_Chr3g0121171 24.453 548 356 14 23 556 584 1087 4.17e-34 139
Msa1021250 MtrunA17_Chr3g0121171 24.906 530 347 13 34 556 27 512 7.99e-34 138
Msa1021250 MtrunA17_Chr3g0121171 23.579 475 312 13 89 556 1798 2228 2.63e-26 114
Msa1021250 MtrunA17_Chr3g0121151 24.453 548 356 14 23 556 49 552 4.84e-35 139
Msa1021250 MtrunA17_Chr3g0121161 24.453 548 356 14 23 556 49 552 9.27e-35 139
Msa1021250 MtrunA17_Chr4g0043261 25.547 548 341 15 34 568 26 519 1.17e-34 137
Msa1021250 MtrunA17_Chr2g0322441 25.926 513 326 15 33 540 30 493 8.71e-34 135
Msa1021250 MtrunA17_Chr5g0402831 24.729 554 347 18 33 571 31 529 6.66e-32 129
Msa1021250 MtrunA17_Chr4g0043251 25.000 520 325 16 51 556 45 513 3.30e-31 127
Msa1021250 MtrunA17_Chr8g0374261 24.861 539 340 19 19 546 13 497 2.54e-30 125
Msa1021250 MtrunA17_Chr4g0075071 25.891 533 346 14 11 540 8 494 2.50e-29 122
Msa1021250 MtrunA17_Chr8g0374271 23.930 514 334 17 33 540 31 493 4.30e-29 121
Msa1021250 MtrunA17_Chr3g0118351 31.928 332 180 10 245 554 5 312 1.84e-28 116
Msa1021250 MtrunA17_Chr3g0118341 39.073 151 89 3 11 159 4 153 9.26e-28 110
Msa1021250 MtrunA17_Chr4g0024641 24.190 463 294 17 85 540 1 413 2.54e-20 95.5
Msa1021250 MtrunA17_Chr1g0197301 27.989 368 213 14 223 580 4 329 7.53e-20 90.9
Msa1021250 MtrunA17_Chr5g0436411 44.444 63 35 0 26 88 17 79 2.19e-14 68.6
Msa1021250 MtrunA17_Chr3g0118931 39.560 91 50 2 490 580 10 95 3.92e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1021250 AT5G60020.1 79.723 577 110 4 11 580 1 577 0.0 960
Msa1021250 AT5G60020.3 82.721 544 89 3 42 580 2 545 0.0 946
Msa1021250 AT5G60020.2 79.065 535 105 4 11 538 1 535 0.0 875
Msa1021250 AT2G29130.1 70.619 582 160 5 1 580 1 573 0.0 862
Msa1021250 AT2G38080.1 57.319 567 225 7 15 580 8 558 0.0 664
Msa1021250 AT5G03260.1 55.185 569 234 9 14 580 8 557 0.0 638
Msa1021250 AT5G58910.2 55.273 550 232 7 33 580 30 567 0.0 634
Msa1021250 AT5G58910.3 55.273 550 231 8 33 580 30 566 0.0 630
Msa1021250 AT1G18140.1 53.036 560 252 5 29 580 25 581 0.0 628
Msa1021250 AT5G01190.1 54.594 566 243 5 15 580 7 558 0.0 619
Msa1021250 AT5G58910.1 54.444 540 225 8 43 580 3 523 0.0 602
Msa1021250 AT5G01190.2 53.887 566 233 6 15 580 7 544 0.0 598
Msa1021250 AT2G40370.1 49.308 578 274 11 15 580 10 580 0.0 565
Msa1021250 AT2G30210.1 49.387 571 275 10 15 580 9 570 0.0 565
Msa1021250 AT5G05390.1 48.348 575 272 10 15 580 7 565 0.0 549
Msa1021250 AT5G09360.1 45.172 580 295 11 4 580 10 569 0.0 540
Msa1021250 AT5G07130.1 47.150 579 286 11 10 580 3 569 0.0 526
Msa1021250 AT2G46570.1 42.784 589 303 12 6 580 1 569 9.65e-174 504
Msa1021250 AT3G09220.1 46.474 553 277 6 17 563 11 550 4.27e-171 498
Msa1021250 AT5G48100.1 44.385 561 295 9 15 570 5 553 5.94e-164 479
Msa1021250 AT5G01040.1 43.031 574 299 9 15 580 13 566 1.45e-159 469
Msa1021250 AT5G01050.1 43.229 576 297 10 15 580 13 568 1.19e-158 466
Msa1021250 AT5G07130.2 47.465 493 243 9 95 580 1 484 1.25e-155 455
Msa1021250 AT5G07130.3 47.465 493 243 9 95 580 1 484 1.25e-155 455
Msa1021250 AT2G46570.2 42.685 499 260 9 90 580 4 484 1.91e-148 437
Msa1021250 AT3G09220.2 47.414 464 226 5 106 563 2 453 2.14e-143 423
Msa1021250 AT5G21105.1 32.496 597 337 19 6 570 11 573 2.97e-75 250
Msa1021250 AT4G39830.1 31.145 594 336 20 16 572 14 571 2.20e-72 243
Msa1021250 AT5G21100.1 32.923 568 310 20 23 559 15 542 6.70e-72 241
Msa1021250 AT5G21105.3 32.206 562 318 18 6 538 11 538 3.81e-66 225
Msa1021250 AT4G39830.2 30.784 510 286 18 94 572 3 476 4.83e-50 180
Msa1021250 AT4G39830.3 30.891 505 282 18 99 572 6 474 1.51e-49 179
Msa1021250 AT1G75790.1 27.684 531 334 17 16 540 9 495 2.43e-48 177
Msa1021250 AT4G12420.1 27.273 550 354 17 15 554 6 519 2.74e-47 175
Msa1021250 AT4G12420.2 27.273 550 354 17 15 554 6 519 2.74e-47 175
Msa1021250 AT4G39830.4 29.862 509 279 17 94 572 3 463 1.43e-46 171
Msa1021250 AT1G21860.1 25.323 541 353 13 8 546 5 496 1.27e-43 164
Msa1021250 AT1G21850.1 26.384 542 346 16 8 546 5 496 2.48e-43 163
Msa1021250 AT5G48450.2 28.309 544 342 23 17 554 12 513 2.61e-43 163
Msa1021250 AT5G48450.1 28.309 544 342 23 17 554 12 513 4.58e-43 163
Msa1021250 AT4G25240.2 26.117 582 379 19 11 580 8 550 7.18e-42 159
Msa1021250 AT4G25240.1 26.117 582 379 19 11 580 8 550 7.18e-42 159
Msa1021250 AT5G21105.2 34.349 361 200 12 218 570 51 382 1.22e-41 155
Msa1021250 AT1G76160.1 25.046 547 359 14 12 556 9 506 1.49e-41 158
Msa1021250 AT1G41830.1 24.126 572 376 17 17 580 10 531 1.74e-40 155
Msa1021250 AT5G51480.1 25.314 557 369 21 35 580 30 550 3.73e-40 154
Msa1021250 AT4G28090.1 24.030 541 354 17 14 546 9 500 2.92e-37 145
Msa1021250 AT4G22010.1 25.271 554 359 17 33 580 26 530 7.69e-37 144
Msa1021250 AT5G66920.1 24.909 550 363 17 33 580 37 538 2.41e-35 140
Msa1021250 AT1G55570.1 25.636 511 336 15 34 540 30 500 2.90e-33 134
Msa1021250 AT4G38420.1 24.514 514 338 17 33 540 30 499 6.06e-33 133
Msa1021250 AT3G13390.1 25.145 517 331 12 34 540 29 499 9.42e-33 132
Msa1021250 AT1G55560.1 24.558 509 341 12 35 540 28 496 4.66e-32 130
Msa1021250 AT4G37160.1 22.971 579 393 13 7 580 4 534 4.47e-30 124
Msa1021250 AT3G13400.2 23.540 531 362 14 15 540 6 497 1.27e-29 123
Msa1021250 AT3G13400.1 23.540 531 362 14 15 540 6 497 1.27e-29 123
Msa1021250 AT4G38420.3 24.597 496 324 17 33 522 30 481 6.37e-29 120
Msa1021250 AT4G38420.2 29.555 247 164 5 33 278 30 267 4.79e-27 114
Msa1021250 AT2G23630.2 24.275 552 361 14 35 580 37 537 6.26e-25 109
Msa1021250 AT2G23630.1 24.275 552 361 14 35 580 32 532 8.43e-25 108
Msa1021250 AT4G37160.2 25.172 290 205 4 7 294 4 283 2.22e-23 103

Find 170 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCGAAGCCTACACAATTAA+TGG 0.188421 7_1:-45557941 Msa1021250:CDS
GTTTACAATTACACAATTAA+AGG 0.195877 7_1:-45558256 Msa1021250:CDS
GGCACAATCTTGTCACATTT+TGG 0.201219 7_1:+45558582 None:intergenic
CTTCTTCAATCACACTTTAC+TGG 0.235780 7_1:-45556835 Msa1021250:CDS
TCTTGGCAGTGCCCAGTTTC+CGG 0.239305 7_1:-45557010 Msa1021250:CDS
AAGGAAGTTCCCATGATATT+TGG 0.245470 7_1:-45558118 Msa1021250:intron
AAGGGACTGTTTGGGAAATA+AGG 0.252706 7_1:+45556790 None:intergenic
TGCCCAGTTGGATAACAAAA+AGG 0.268658 7_1:+45556165 None:intergenic
GGCTTTAACTTCTTTGTTGT+TGG 0.273389 7_1:-45556616 Msa1021250:CDS
AGAGATTTATTTATACAATA+TGG 0.277397 7_1:-45556048 Msa1021250:three_prime_UTR
CACAACTGTTGCTGGTATCT+TGG 0.309201 7_1:-45557160 Msa1021250:CDS
TGGTAACTACGATTCAAATA+AGG 0.314009 7_1:-45556584 Msa1021250:CDS
GCCAAAAGAGTGTTCCTCTT+TGG 0.315527 7_1:+45558233 None:intergenic
GTGGAACGTTAGCCGGAAAC+TGG 0.316439 7_1:+45556998 None:intergenic
TTCAAGGAAGTACATCAAAT+TGG 0.316469 7_1:+45558699 None:intergenic
TGGTTTGGCCAGGGGCTATA+AGG 0.321290 7_1:+45557277 None:intergenic
GCCAAAGAGGAACACTCTTT+TGG 0.325478 7_1:-45558234 Msa1021250:CDS
TTTGACAACACAACTGTTGC+TGG 0.325687 7_1:-45557168 Msa1021250:CDS
TTTGTTGTTGGACAAGGATT+TGG 0.332895 7_1:-45556604 Msa1021250:CDS
CATTGGGTCCCTGACAAGTT+TGG 0.333389 7_1:+45556914 None:intergenic
AAACATAGCTTTGACCTGTT+TGG 0.349503 7_1:+45558275 None:intergenic
GTTTCCAGGACCTCGCATTA+TGG 0.352691 7_1:-45558528 Msa1021250:CDS
TACACAATTAATGGACTTCC+AGG 0.359750 7_1:-45557932 Msa1021250:CDS
TTTGGCATGCTCACATATCT+TGG 0.374938 7_1:-45558216 Msa1021250:CDS
CGTAGAAGGTAAGTCTTTCC+AGG 0.379445 7_1:+45557411 None:intergenic
AATGGATGCAAGGGACTGTT+TGG 0.382635 7_1:+45556781 None:intergenic
GAGTTTGAACTGTGAGTTGA+TGG 0.387802 7_1:+45558881 None:intergenic
CCAATTGTATTCCTTTCAAC+AGG 0.394251 7_1:+45556535 None:intergenic
AGGGACTGTTTGGGAAATAA+GGG 0.397523 7_1:+45556791 None:intergenic
TGAGAAGAACATTAGTGGTT+TGG 0.400081 7_1:+45557262 None:intergenic
TCCATTAATTGTGTAGGCTT+CGG 0.403306 7_1:+45557940 None:intergenic
TAATTGTGTGTGCAGGAGTA+TGG 0.404396 7_1:-45556367 Msa1021250:intron
TGGTTAAGATCAACACTCTA+TGG 0.410307 7_1:-45558196 Msa1021250:CDS
TTGCAGGGCTTGTGCTATGA+TGG 0.413663 7_1:+45557985 None:intergenic
ACAGTGAACGGTCAGTTTCC+AGG 0.413798 7_1:-45558542 Msa1021250:CDS
AACTTCATTACCAAATATCA+TGG 0.417572 7_1:+45558108 None:intergenic
ATAGCACAAGCCCTGCAAAC+TGG 0.417808 7_1:-45557980 Msa1021250:CDS
TCAAAGCAACCAAACTTGTC+AGG 0.420571 7_1:-45556923 Msa1021250:CDS
GGTGTTGTTTGGTGGGGTGC+CGG 0.425328 7_1:+45556750 None:intergenic
GCGGAATGATGAGTCTTTGA+TGG 0.425975 7_1:+45557126 None:intergenic
TTGCTCACCATGGTGTTGTT+TGG 0.428117 7_1:+45556739 None:intergenic
GTGTAGGCTTCGGAAACATT+TGG 0.435373 7_1:+45557950 None:intergenic
CTCATTAAGAAGGCTGCATT+GGG 0.435807 7_1:+45557222 None:intergenic
CTCACCATGGTGTTGTTTGG+TGG 0.438016 7_1:+45556742 None:intergenic
TACAGATACATTCAAGCTAA+AGG 0.442726 7_1:-45557439 Msa1021250:intron
TAGCACAAGCCCTGCAAACT+GGG 0.444789 7_1:-45557979 Msa1021250:CDS
CGTCGGCTTCAACAACTTTG+AGG 0.450966 7_1:+45557334 None:intergenic
ATCGGGATGGGCTGATGGAC+CGG 0.451746 7_1:-45558320 Msa1021250:CDS
CTGCAAGACACTAGCATCCT+TGG 0.453652 7_1:-45556667 Msa1021250:CDS
TGGCATTCGACAACTTCAAT+CGG 0.454278 7_1:-45558338 Msa1021250:CDS
GCATTTGAGCTTGCAATGGC+CGG 0.454548 7_1:-45558737 Msa1021250:CDS
AAAACAACATCTCGATTCAT+TGG 0.455822 7_1:-45558460 Msa1021250:intron
GTTCCCTGGCCGGTAGCATA+TGG 0.460544 7_1:+45557192 None:intergenic
CTTGGAAGTACATACAAGTT+GGG 0.464129 7_1:-45556330 Msa1021250:CDS
TCACCATGGTGTTGTTTGGT+GGG 0.464819 7_1:+45556743 None:intergenic
ATGAATAACAATTGCCCAGT+TGG 0.465681 7_1:+45556153 None:intergenic
AGAACATTAGTGGTTTGGCC+AGG 0.466664 7_1:+45557267 None:intergenic
ACTCATTAAGAAGGCTGCAT+TGG 0.468803 7_1:+45557221 None:intergenic
GGTCTTGAGAAGAACATTAG+TGG 0.469300 7_1:+45557257 None:intergenic
CAGCGTTGATCAAACGTAGA+AGG 0.469718 7_1:+45557397 None:intergenic
ACTTTCTGTGGAACGTTAGC+CGG 0.470540 7_1:+45556991 None:intergenic
AGGATGGTGTTAGTCTCAAA+AGG 0.471837 7_1:+45557297 None:intergenic
AGCTTTGACCTGTTTGGATA+GGG 0.473589 7_1:+45558281 None:intergenic
CAAGTTTGGTTGCTTTGACA+AGG 0.474522 7_1:+45556928 None:intergenic
CTTCAATCGGGATGGGCTGA+TGG 0.474574 7_1:-45558325 Msa1021250:CDS
TACATCAAATTGGTAGTGTC+TGG 0.475328 7_1:+45558709 None:intergenic
TGCTGCAAACATTGTTCCAT+TGG 0.477184 7_1:+45556897 None:intergenic
CTAGCCATAATGCGAGGTCC+TGG 0.477886 7_1:+45558524 None:intergenic
AAAGAAGTGTCATTAAGTGC+TGG 0.478611 7_1:+45557063 None:intergenic
TTGGTGTCCCGTCTGGTGGA+TGG 0.481797 7_1:-45556516 Msa1021250:CDS
TAGCTTTGACCTGTTTGGAT+AGG 0.482427 7_1:+45558280 None:intergenic
AACTTCTTTGTTGTTGGACA+AGG 0.483301 7_1:-45556610 Msa1021250:CDS
ACTCAATGCCCTATCCAAAC+AGG 0.485770 7_1:-45558289 Msa1021250:CDS
AATACAATTGGTGTCCCGTC+TGG 0.486060 7_1:-45556523 Msa1021250:CDS
AGAAGTTAAAGCCATGCAAA+TGG 0.489593 7_1:+45556626 None:intergenic
GGGAGAGTAGGTTTGAATAG+TGG 0.491888 7_1:+45557084 None:intergenic
CTGTCACCATCCTCTTGTTG+TGG 0.500041 7_1:+45558561 None:intergenic
GAGCAGTTATACAATGGCCC+TGG 0.502633 7_1:+45557914 None:intergenic
TATCATGGGAACTTCCTTGT+GGG 0.505192 7_1:+45558123 None:intergenic
TCGAAGAAACTCCGTAGTCT+TGG 0.509213 7_1:-45557027 Msa1021250:CDS
GTCGGCTTCAACAACTTTGA+GGG 0.510915 7_1:+45557335 None:intergenic
TTCAAGCTAAAGGTCAAGCC+TGG 0.511697 7_1:-45557429 Msa1021250:CDS
GAACATTAGTGGTTTGGCCA+GGG 0.511967 7_1:+45557268 None:intergenic
TTGGTTGCTTTGACAAGGGT+TGG 0.514248 7_1:+45556933 None:intergenic
TAACACTTGGGAAGATCAGC+TGG 0.519647 7_1:+45556245 None:intergenic
ACACTTTACTGGACAATCCA+AGG 0.522053 7_1:-45556824 Msa1021250:CDS
GCTGCAAACATTGTTCCATT+GGG 0.522090 7_1:+45556898 None:intergenic
GGGGTGCCGGTGTAATTGAA+TGG 0.523303 7_1:+45556763 None:intergenic
TGGTGTCCCGTCTGGTGGAT+GGG 0.524205 7_1:-45556515 Msa1021250:CDS
TTGCGCATTTGAGCTTGCAA+TGG 0.524285 7_1:-45558741 Msa1021250:CDS
ATGGATGCAAGGGACTGTTT+GGG 0.525250 7_1:+45556782 None:intergenic
TTGGTAGTGTCTGGTAGTGC+CGG 0.526075 7_1:+45558718 None:intergenic
ACTTGGAAGTACATACAAGT+TGG 0.526801 7_1:-45556331 Msa1021250:CDS
ATGGTTCATGCATTGTCACT+TGG 0.529655 7_1:-45556348 Msa1021250:CDS
TCATTAAGTGCTGGGAGAGT+AGG 0.529980 7_1:+45557072 None:intergenic
TACAAGTTGGGGTCTTAAGA+TGG 0.531380 7_1:-45556318 Msa1021250:CDS
CGGAATGATGAGTCTTTGAT+GGG 0.531487 7_1:+45557127 None:intergenic
AAGAAGTGTCATTAAGTGCT+GGG 0.532392 7_1:+45557064 None:intergenic
CACCATGGTGTTGTTTGGTG+GGG 0.534985 7_1:+45556744 None:intergenic
CAAGATTGTGCCACAACAAG+AGG 0.537997 7_1:-45558571 Msa1021250:CDS
AAGTTTGGTTGCTTTGACAA+GGG 0.538040 7_1:+45556929 None:intergenic
TTCGACAACTTCAATCGGGA+TGG 0.539086 7_1:-45558333 Msa1021250:CDS
AACACCATCCTTATAGCCCC+TGG 0.539383 7_1:-45557285 Msa1021250:CDS
GGCATTCGACAACTTCAATC+GGG 0.539404 7_1:-45558337 Msa1021250:CDS
TGGTCTAGCACTCATTAAGA+AGG 0.539912 7_1:+45557212 None:intergenic
TTGGGAAGATCAGCTGGTGG+TGG 0.542541 7_1:+45556251 None:intergenic
CCTGTTGAAAGGAATACAAT+TGG 0.547051 7_1:-45556535 Msa1021250:CDS
ATAAGGGGCGTAGACACCCT+TGG 0.547982 7_1:+45556807 None:intergenic
AGCTCCACACTTGTGTTGAA+AGG 0.548871 7_1:+45556694 None:intergenic
TAAGATGGCATGGATCGTCT+TGG 0.549615 7_1:-45556303 Msa1021250:CDS
AACATTAGTGGTTTGGCCAG+GGG 0.551663 7_1:+45557269 None:intergenic
AAGTGCTTATCAACTTTCTG+TGG 0.551982 7_1:+45556979 None:intergenic
TTGTGTTGTCAAAAGTTCCC+TGG 0.552350 7_1:+45557178 None:intergenic
TTTAGGTTGAAGTTAACACT+TGG 0.558796 7_1:+45556232 None:intergenic
ATGGCATGGATCGTCTTGGA+TGG 0.560159 7_1:-45556299 Msa1021250:CDS
TGGAAGTCCATTAATTGTGT+AGG 0.560908 7_1:+45557934 None:intergenic
AGTCATCCTCTCCATTTGCA+TGG 0.564575 7_1:-45556637 Msa1021250:CDS
ACCTTGTTGATCCTGTTGAA+AGG 0.566819 7_1:-45556546 Msa1021250:CDS
TTTGACAAGGGTTGGTACCA+AGG 0.568017 7_1:+45556941 None:intergenic
CTTTGTTCCGTTGCTCACCA+TGG 0.570394 7_1:+45556729 None:intergenic
TTCTTGGGAAGAATGATGAG+AGG 0.570952 7_1:+45558172 None:intergenic
GGGCATTGAGTCACATATGC+CGG 0.574468 7_1:+45558301 None:intergenic
GAGCAACGGAACAAAGGTTA+CGG 0.576807 7_1:-45556722 Msa1021250:CDS
TAGACCATATGCTACCGGCC+AGG 0.578944 7_1:-45557196 Msa1021250:CDS
TGCATTGGGATAGTGTGACT+TGG 0.579231 7_1:+45557236 None:intergenic
CAAACTTGTCAGGGACCCAA+TGG 0.579816 7_1:-45556913 Msa1021250:CDS
GTTGTCAAAAGTTCCCTGGC+CGG 0.582928 7_1:+45557182 None:intergenic
TGGAACGTTAGCCGGAAACT+GGG 0.583850 7_1:+45556999 None:intergenic
AAGAGGATGGTGACAGTGAA+CGG 0.583975 7_1:-45558554 Msa1021250:CDS
TACACAATTAAAGGCCAAAG+AGG 0.584584 7_1:-45558247 Msa1021250:CDS
ACTGGGCACTGCCAAGACTA+CGG 0.584704 7_1:+45557016 None:intergenic
GTTGGGGTCTTAAGATGGCA+TGG 0.588905 7_1:-45556313 Msa1021250:CDS
TCTTCCTTTCAACACAAGTG+TGG 0.589113 7_1:-45556698 Msa1021250:CDS
AACAACACCATGGTGAGCAA+CGG 0.589304 7_1:-45556736 Msa1021250:CDS
ACCAACCTGGATTATCTGCT+AGG 0.591178 7_1:+45556479 None:intergenic
AGCACAAGCCCTGCAAACTG+GGG 0.594583 7_1:-45557978 Msa1021250:CDS
AGGTTTCACATAAATAGCGT+CGG 0.595219 7_1:+45557317 None:intergenic
TCGACAACTTCAATCGGGAT+GGG 0.595443 7_1:-45558332 Msa1021250:CDS
AGACCATATGCTACCGGCCA+GGG 0.596614 7_1:-45557195 Msa1021250:CDS
ATCATGGGAACTTCCTTGTG+GGG 0.597762 7_1:+45558124 None:intergenic
ACAATTGGTGTCCCGTCTGG+TGG 0.601621 7_1:-45556520 Msa1021250:CDS
AGATTCCTAGCAGATAATCC+AGG 0.604726 7_1:-45556484 Msa1021250:intron
TAGCAACCCATCCACCAGAC+GGG 0.606966 7_1:+45556509 None:intergenic
CCTCGCATTATGGCTAGAGA+AGG 0.607407 7_1:-45558518 Msa1021250:CDS
ACTTCATTACCAAATATCAT+GGG 0.608410 7_1:+45558109 None:intergenic
CATGGTGAGCAACGGAACAA+AGG 0.608494 7_1:-45556728 Msa1021250:CDS
AGTGCTAGACCATATGCTAC+CGG 0.615170 7_1:-45557201 Msa1021250:CDS
GATTGAAGAAGTGCGGTGGT+TGG 0.616158 7_1:+45556847 None:intergenic
TTGCATCCATTCAATTACAC+CGG 0.616954 7_1:-45556769 Msa1021250:CDS
GTGTAATTGAATGGATGCAA+GGG 0.617657 7_1:+45556772 None:intergenic
CAAAGCAACCAAACTTGTCA+GGG 0.619064 7_1:-45556922 Msa1021250:CDS
TCCTTTCAACAGGATCAACA+AGG 0.625266 7_1:+45556545 None:intergenic
TTAGGTTGAAGTTAACACTT+GGG 0.632484 7_1:+45556233 None:intergenic
ATAGCAACCCATCCACCAGA+CGG 0.634950 7_1:+45556508 None:intergenic
TTGGAAGTACATACAAGTTG+GGG 0.638295 7_1:-45556329 Msa1021250:CDS
GGGACTGTTTGGGAAATAAG+GGG 0.641737 7_1:+45556792 None:intergenic
GAGGATGACTTTCAGCACCA+AGG 0.642480 7_1:+45556650 None:intergenic
AGATCGTCTTGTTATCAAAG+TGG 0.647826 7_1:-45558495 Msa1021250:CDS
ATATCATGGGAACTTCCTTG+TGG 0.653096 7_1:+45558122 None:intergenic
ATTGTGCCACAACAAGAGGA+TGG 0.654518 7_1:-45558567 Msa1021250:CDS
CACTTTACTGGACAATCCAA+GGG 0.654690 7_1:-45556823 Msa1021250:CDS
CACCCCACCAAACAACACCA+TGG 0.657757 7_1:-45556746 Msa1021250:CDS
TTAAAGCCATGCAAATGGAG+AGG 0.661539 7_1:+45556631 None:intergenic
ACACAATTAATGGACTTCCA+GGG 0.662456 7_1:-45557931 Msa1021250:CDS
GCACAAGCCCTGCAAACTGG+GGG 0.674904 7_1:-45557977 Msa1021250:CDS
GGTGTAATTGAATGGATGCA+AGG 0.689847 7_1:+45556771 None:intergenic
CACTTGGGAAGATCAGCTGG+TGG 0.691975 7_1:+45556248 None:intergenic
CCTTCTCTAGCCATAATGCG+AGG 0.707124 7_1:+45558518 None:intergenic
GTGTGATTGAAGAAGTGCGG+TGG 0.708370 7_1:+45556843 None:intergenic
AAAGTGTGATTGAAGAAGTG+CGG 0.738535 7_1:+45556840 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTGTAATAAATATTTCA+AGG + chr7_1:45556214-45556233 None:intergenic 15.0%
!! AGCTTATGTAAATATAATAT+TGG + chr7_1:45556835-45556854 None:intergenic 15.0%
!!! CTAATTATACTCTTTAATTT+TGG - chr7_1:45556853-45556872 Msa1021250:CDS 15.0%
!!! ATGAATTTTGATATAAAACT+TGG + chr7_1:45557271-45557290 None:intergenic 15.0%
!! ATATAGAATTCTTTATGAAA+CGG - chr7_1:45557391-45557410 Msa1021250:CDS 15.0%
!! AGAGATTTATTTATACAATA+TGG - chr7_1:45558846-45558865 Msa1021250:five_prime_UTR 15.0%
!!! AATTTTAAGCGATGAAAAAA+TGG + chr7_1:45556104-45556123 None:intergenic 20.0%
!! GTTTACAATTACACAATTAA+AGG - chr7_1:45556638-45556657 Msa1021250:CDS 20.0%
!! AATATAATATTGGAAGACTT+AGG + chr7_1:45556825-45556844 None:intergenic 20.0%
!!! TACTCTTTAATTTTGGTTTT+AGG - chr7_1:45556860-45556879 Msa1021250:CDS 20.0%
!! TGCATATAAATATATCTTCT+AGG - chr7_1:45557019-45557038 Msa1021250:CDS 20.0%
!! CACCAATTATTAAAAAATTG+AGG - chr7_1:45557048-45557067 Msa1021250:CDS 20.0%
!!! TGCCTCAATTTTTTAATAAT+TGG + chr7_1:45557053-45557072 None:intergenic 20.0%
!!! TAAAGAAAAGAAGCAATTTT+AGG + chr7_1:45558682-45558701 None:intergenic 20.0%
!!! TTTTCTGTAATGGTTTTTTA+AGG - chr7_1:45558780-45558799 Msa1021250:CDS 20.0%
!!! GTTACATATTTTTCTTTCTT+TGG - chr7_1:45558820-45558839 Msa1021250:exon 20.0%
! AATGTAACGTGTAAAAAACT+TGG - chr7_1:45556515-45556534 Msa1021250:CDS 25.0%
! ACTTCATTACCAAATATCAT+GGG + chr7_1:45556788-45556807 None:intergenic 25.0%
! AACTTCATTACCAAATATCA+TGG + chr7_1:45556789-45556808 None:intergenic 25.0%
!!! TAATTTTGGTTTTAGGTGAA+TGG - chr7_1:45556867-45556886 Msa1021250:CDS 25.0%
!!! TTTTTAGAGCAGTTATACAA+TGG + chr7_1:45556989-45557008 None:intergenic 25.0%
!!! TTGTATAACTGCTCTAAAAA+AGG - chr7_1:45556989-45557008 Msa1021250:CDS 25.0%
!!! GCTTCATATTTTCAAGTTTA+GGG + chr7_1:45557082-45557101 None:intergenic 25.0%
! ATTATGAGTAGTTACGTTTA+AGG + chr7_1:45557104-45557123 None:intergenic 25.0%
!! TATTTGCTCTTTTGATAAAG+TGG + chr7_1:45557311-45557330 None:intergenic 25.0%
!!! CAACAAGGTTAAAGTTTTTT+GGG + chr7_1:45558337-45558356 None:intergenic 25.0%
!!! TCAACAAGGTTAAAGTTTTT+TGG + chr7_1:45558338-45558357 None:intergenic 25.0%
! GATAGTTTGATGAATAAACT+AGG + chr7_1:45558446-45558465 None:intergenic 25.0%
!! CAAACTATCAAAAGTCATTT+TGG - chr7_1:45558457-45558476 Msa1021250:intron 25.0%
!!! CTTTAGTTTCTTTTCTGTAA+TGG - chr7_1:45558770-45558789 Msa1021250:CDS 25.0%
CTCATTCAGAGAGAAAAAAA+TGG - chr7_1:45556072-45556091 Msa1021250:three_prime_UTR 30.0%
! TTCAAGGAAGTACATCAAAT+TGG + chr7_1:45556198-45556217 None:intergenic 30.0%
TACTTTGCTTGTAACATATG+AGG - chr7_1:45556269-45556288 Msa1021250:CDS 30.0%
ATGAGGTTATTTATGCAGAT+AGG - chr7_1:45556286-45556305 Msa1021250:CDS 30.0%
TGAGGTTATTTATGCAGATA+GGG - chr7_1:45556287-45556306 Msa1021250:CDS 30.0%
AAAACAACATCTCGATTCAT+TGG - chr7_1:45556434-45556453 Msa1021250:intron 30.0%
!! AACTTGGCTATCTTTGAAAT+AGG - chr7_1:45556531-45556550 Msa1021250:CDS 30.0%
! GGATATGGAACATTTTTCTT+GGG + chr7_1:45556740-45556759 None:intergenic 30.0%
! AGGATATGGAACATTTTTCT+TGG + chr7_1:45556741-45556760 None:intergenic 30.0%
!!! GGCTTCATATTTTCAAGTTT+AGG + chr7_1:45557083-45557102 None:intergenic 30.0%
TAAAACAGACACTCAAAAAG+TGG - chr7_1:45557332-45557351 Msa1021250:CDS 30.0%
! CTATATGCTTTTGTCGAAAT+TGG + chr7_1:45557377-45557396 None:intergenic 30.0%
TACAGATACATTCAAGCTAA+AGG - chr7_1:45557455-45557474 Msa1021250:intron 30.0%
!!! AAGCTTTTTTAGTGAAGAAG+CGG + chr7_1:45557790-45557809 None:intergenic 30.0%
TGGTAACTACGATTCAAATA+AGG - chr7_1:45558310-45558329 Msa1021250:CDS 30.0%
GTTTGATGAATAAACTAGGA+AGG + chr7_1:45558442-45558461 None:intergenic 30.0%
!! TTAGGTTGAAGTTAACACTT+GGG + chr7_1:45558664-45558683 None:intergenic 30.0%
!! TTTAGGTTGAAGTTAACACT+TGG + chr7_1:45558665-45558684 None:intergenic 30.0%
! GGAATTTTCACTCAAATCAA+TGG + chr7_1:45558892-45558911 None:intergenic 30.0%
!! TACATCAAATTGGTAGTGTC+TGG + chr7_1:45556188-45556207 None:intergenic 35.0%
! AGATCGTCTTGTTATCAAAG+TGG - chr7_1:45556399-45556418 Msa1021250:intron 35.0%
!! AAACATAGCTTTGACCTGTT+TGG + chr7_1:45556622-45556641 None:intergenic 35.0%
TACACAATTAAAGGCCAAAG+AGG - chr7_1:45556647-45556666 Msa1021250:CDS 35.0%
TGGTTAAGATCAACACTCTA+TGG - chr7_1:45556698-45556717 Msa1021250:CDS 35.0%
AAGGAAGTTCCCATGATATT+TGG - chr7_1:45556776-45556795 Msa1021250:CDS 35.0%
TCCATTAATTGTGTAGGCTT+CGG + chr7_1:45556957-45556976 None:intergenic 35.0%
TGGAAGTCCATTAATTGTGT+AGG + chr7_1:45556963-45556982 None:intergenic 35.0%
!! TACACAATTAATGGACTTCC+AGG - chr7_1:45556962-45556981 Msa1021250:CDS 35.0%
!! ACACAATTAATGGACTTCCA+GGG - chr7_1:45556963-45556982 Msa1021250:CDS 35.0%
ATGAAACGGTATCAACACTA+TGG - chr7_1:45557405-45557424 Msa1021250:CDS 35.0%
AGGTTTCACATAAATAGCGT+CGG + chr7_1:45557580-45557599 None:intergenic 35.0%
TGAGAAGAACATTAGTGGTT+TGG + chr7_1:45557635-45557654 None:intergenic 35.0%
!! AAGAAGTGTCATTAAGTGCT+GGG + chr7_1:45557833-45557852 None:intergenic 35.0%
!! AAAGAAGTGTCATTAAGTGC+TGG + chr7_1:45557834-45557853 None:intergenic 35.0%
AAGTGCTTATCAACTTTCTG+TGG + chr7_1:45557918-45557937 None:intergenic 35.0%
! TTTTTCTTCACAGTAAGCCT+TGG - chr7_1:45557936-45557955 Msa1021250:CDS 35.0%
! AAGTTTGGTTGCTTTGACAA+GGG + chr7_1:45557968-45557987 None:intergenic 35.0%
AAAGTGTGATTGAAGAAGTG+CGG + chr7_1:45558057-45558076 None:intergenic 35.0%
CTTCTTCAATCACACTTTAC+TGG - chr7_1:45558059-45558078 Msa1021250:intron 35.0%
GTGTAATTGAATGGATGCAA+GGG + chr7_1:45558125-45558144 None:intergenic 35.0%
TTGCATCCATTCAATTACAC+CGG - chr7_1:45558125-45558144 Msa1021250:CDS 35.0%
AGAAGTTAAAGCCATGCAAA+TGG + chr7_1:45558271-45558290 None:intergenic 35.0%
! GGCTTTAACTTCTTTGTTGT+TGG - chr7_1:45558278-45558297 Msa1021250:CDS 35.0%
!! AACTTCTTTGTTGTTGGACA+AGG - chr7_1:45558284-45558303 Msa1021250:CDS 35.0%
!! TTTGTTGTTGGACAAGGATT+TGG - chr7_1:45558290-45558309 Msa1021250:CDS 35.0%
CCAATTGTATTCCTTTCAAC+AGG + chr7_1:45558362-45558381 None:intergenic 35.0%
CCTGTTGAAAGGAATACAAT+TGG - chr7_1:45558359-45558378 Msa1021250:intron 35.0%
AATGTTCTAATTGTGTGTGC+AGG - chr7_1:45558520-45558539 Msa1021250:CDS 35.0%
! ACTTGGAAGTACATACAAGT+TGG - chr7_1:45558563-45558582 Msa1021250:CDS 35.0%
! CTTGGAAGTACATACAAGTT+GGG - chr7_1:45558564-45558583 Msa1021250:CDS 35.0%
! TTGGAAGTACATACAAGTTG+GGG - chr7_1:45558565-45558584 Msa1021250:CDS 35.0%
!! GGTGGAAGTAACTTTTGATT+TGG + chr7_1:45558628-45558647 None:intergenic 35.0%
ATGAATAACAATTGCCCAGT+TGG + chr7_1:45558744-45558763 None:intergenic 35.0%
! GAGTTTGAACTGTGAGTTGA+TGG + chr7_1:45556016-45556035 None:intergenic 40.0%
! GGCACAATCTTGTCACATTT+TGG + chr7_1:45556315-45556334 None:intergenic 40.0%
!! GGCTATCTTTGAAATAGGCA+TGG - chr7_1:45556536-45556555 Msa1021250:CDS 40.0%
TGGCATTCGACAACTTCAAT+CGG - chr7_1:45556556-45556575 Msa1021250:CDS 40.0%
! AGCTTTGACCTGTTTGGATA+GGG + chr7_1:45556616-45556635 None:intergenic 40.0%
!! TAGCTTTGACCTGTTTGGAT+AGG + chr7_1:45556617-45556636 None:intergenic 40.0%
TTTGGCATGCTCACATATCT+TGG - chr7_1:45556678-45556697 Msa1021250:CDS 40.0%
TTCTTGGGAAGAATGATGAG+AGG + chr7_1:45556725-45556744 None:intergenic 40.0%
! TGGGGTTTTGCAAAAGGATA+TGG + chr7_1:45556755-45556774 None:intergenic 40.0%
TATCATGGGAACTTCCTTGT+GGG + chr7_1:45556774-45556793 None:intergenic 40.0%
ATATCATGGGAACTTCCTTG+TGG + chr7_1:45556775-45556794 None:intergenic 40.0%
TCCGAAGCCTACACAATTAA+TGG - chr7_1:45556953-45556972 Msa1021250:CDS 40.0%
CTTGGATGTCGAATATAGCA+TGG + chr7_1:45557253-45557272 None:intergenic 40.0%
! AGGATGGTGTTAGTCTCAAA+AGG + chr7_1:45557600-45557619 None:intergenic 40.0%
GGTCTTGAGAAGAACATTAG+TGG + chr7_1:45557640-45557659 None:intergenic 40.0%
CTCATTAAGAAGGCTGCATT+GGG + chr7_1:45557675-45557694 None:intergenic 40.0%
ACTCATTAAGAAGGCTGCAT+TGG + chr7_1:45557676-45557695 None:intergenic 40.0%
TGGTCTAGCACTCATTAAGA+AGG + chr7_1:45557685-45557704 None:intergenic 40.0%
TTGTGTTGTCAAAAGTTCCC+TGG + chr7_1:45557719-45557738 None:intergenic 40.0%
TTTGACAACACAACTGTTGC+TGG - chr7_1:45557726-45557745 Msa1021250:intron 40.0%
CGGAATGATGAGTCTTTGAT+GGG + chr7_1:45557770-45557789 None:intergenic 40.0%
! CAAGTTTGGTTGCTTTGACA+AGG + chr7_1:45557969-45557988 None:intergenic 40.0%
TCAAAGCAACCAAACTTGTC+AGG - chr7_1:45557971-45557990 Msa1021250:CDS 40.0%
CAAAGCAACCAAACTTGTCA+GGG - chr7_1:45557972-45557991 Msa1021250:CDS 40.0%
GCTGCAAACATTGTTCCATT+GGG + chr7_1:45557999-45558018 None:intergenic 40.0%
TGCTGCAAACATTGTTCCAT+TGG + chr7_1:45558000-45558019 None:intergenic 40.0%
ACACTTTACTGGACAATCCA+AGG - chr7_1:45558070-45558089 Msa1021250:intron 40.0%
CACTTTACTGGACAATCCAA+GGG - chr7_1:45558071-45558090 Msa1021250:intron 40.0%
AGGGACTGTTTGGGAAATAA+GGG + chr7_1:45558106-45558125 None:intergenic 40.0%
AAGGGACTGTTTGGGAAATA+AGG + chr7_1:45558107-45558126 None:intergenic 40.0%
GGTGTAATTGAATGGATGCA+AGG + chr7_1:45558126-45558145 None:intergenic 40.0%
TCTTCCTTTCAACACAAGTG+TGG - chr7_1:45558196-45558215 Msa1021250:CDS 40.0%
TTAAAGCCATGCAAATGGAG+AGG + chr7_1:45558266-45558285 None:intergenic 40.0%
ACCTTGTTGATCCTGTTGAA+AGG - chr7_1:45558348-45558367 Msa1021250:intron 40.0%
TCCTTTCAACAGGATCAACA+AGG + chr7_1:45558352-45558371 None:intergenic 40.0%
AGATTCCTAGCAGATAATCC+AGG - chr7_1:45558410-45558429 Msa1021250:intron 40.0%
TAATTGTGTGTGCAGGAGTA+TGG - chr7_1:45558527-45558546 Msa1021250:CDS 40.0%
ATGGTTCATGCATTGTCACT+TGG - chr7_1:45558546-45558565 Msa1021250:CDS 40.0%
! TACAAGTTGGGGTCTTAAGA+TGG - chr7_1:45558576-45558595 Msa1021250:CDS 40.0%
!! GCCCTTTTTGTTATCCAACT+GGG - chr7_1:45558727-45558746 Msa1021250:CDS 40.0%
GCCCAGTTGGATAACAAAAA+GGG + chr7_1:45558731-45558750 None:intergenic 40.0%
TGCCCAGTTGGATAACAAAA+AGG + chr7_1:45558732-45558751 None:intergenic 40.0%
!!! GTTTTTTAAGGCTTGACGTG+AGG - chr7_1:45558792-45558811 Msa1021250:CDS 40.0%
TTGCGCATTTGAGCTTGCAA+TGG - chr7_1:45556153-45556172 Msa1021250:three_prime_UTR 45.0%
CAAGATTGTGCCACAACAAG+AGG - chr7_1:45556323-45556342 Msa1021250:CDS 45.0%
ATTGTGCCACAACAAGAGGA+TGG - chr7_1:45556327-45556346 Msa1021250:CDS 45.0%
AAGAGGATGGTGACAGTGAA+CGG - chr7_1:45556340-45556359 Msa1021250:CDS 45.0%
GGCATTCGACAACTTCAATC+GGG - chr7_1:45556557-45556576 Msa1021250:CDS 45.0%
TTCGACAACTTCAATCGGGA+TGG - chr7_1:45556561-45556580 Msa1021250:CDS 45.0%
TCGACAACTTCAATCGGGAT+GGG - chr7_1:45556562-45556581 Msa1021250:CDS 45.0%
ACTCAATGCCCTATCCAAAC+AGG - chr7_1:45556605-45556624 Msa1021250:CDS 45.0%
! GCCAAAGAGGAACACTCTTT+TGG - chr7_1:45556660-45556679 Msa1021250:CDS 45.0%
!! GCCAAAAGAGTGTTCCTCTT+TGG + chr7_1:45556664-45556683 None:intergenic 45.0%
! TCCTTTTGCAAAACCCCACA+AGG - chr7_1:45556757-45556776 Msa1021250:CDS 45.0%
! TCCTTGTGGGGTTTTGCAAA+AGG + chr7_1:45556761-45556780 None:intergenic 45.0%
ATCATGGGAACTTCCTTGTG+GGG + chr7_1:45556773-45556792 None:intergenic 45.0%
GTGTAGGCTTCGGAAACATT+TGG + chr7_1:45556947-45556966 None:intergenic 45.0%
TTCAAGCTAAAGGTCAAGCC+TGG - chr7_1:45557465-45557484 Msa1021250:intron 45.0%
CGTAGAAGGTAAGTCTTTCC+AGG + chr7_1:45557486-45557505 None:intergenic 45.0%
!! CAGCGTTGATCAAACGTAGA+AGG + chr7_1:45557500-45557519 None:intergenic 45.0%
! GTCGGCTTCAACAACTTTGA+GGG + chr7_1:45557562-45557581 None:intergenic 45.0%
! AACATTAGTGGTTTGGCCAG+GGG + chr7_1:45557628-45557647 None:intergenic 45.0%
! GAACATTAGTGGTTTGGCCA+GGG + chr7_1:45557629-45557648 None:intergenic 45.0%
! AGAACATTAGTGGTTTGGCC+AGG + chr7_1:45557630-45557649 None:intergenic 45.0%
TGCATTGGGATAGTGTGACT+TGG + chr7_1:45557661-45557680 None:intergenic 45.0%
AGTGCTAGACCATATGCTAC+CGG - chr7_1:45557693-45557712 Msa1021250:intron 45.0%
CACAACTGTTGCTGGTATCT+TGG - chr7_1:45557734-45557753 Msa1021250:intron 45.0%
GCGGAATGATGAGTCTTTGA+TGG + chr7_1:45557771-45557790 None:intergenic 45.0%
GGGAGAGTAGGTTTGAATAG+TGG + chr7_1:45557813-45557832 None:intergenic 45.0%
!! TCATTAAGTGCTGGGAGAGT+AGG + chr7_1:45557825-45557844 None:intergenic 45.0%
TCGAAGAAACTCCGTAGTCT+TGG - chr7_1:45557867-45557886 Msa1021250:intron 45.0%
ACTTTCTGTGGAACGTTAGC+CGG + chr7_1:45557906-45557925 None:intergenic 45.0%
!! TTTGACAAGGGTTGGTACCA+AGG + chr7_1:45557956-45557975 None:intergenic 45.0%
! TTGGTTGCTTTGACAAGGGT+TGG + chr7_1:45557964-45557983 None:intergenic 45.0%
GGGACTGTTTGGGAAATAAG+GGG + chr7_1:45558105-45558124 None:intergenic 45.0%
ATGGATGCAAGGGACTGTTT+GGG + chr7_1:45558115-45558134 None:intergenic 45.0%
AATGGATGCAAGGGACTGTT+TGG + chr7_1:45558116-45558135 None:intergenic 45.0%
!! TCACCATGGTGTTGTTTGGT+GGG + chr7_1:45558154-45558173 None:intergenic 45.0%
!! TTGCTCACCATGGTGTTGTT+TGG + chr7_1:45558158-45558177 None:intergenic 45.0%
AACAACACCATGGTGAGCAA+CGG - chr7_1:45558158-45558177 Msa1021250:CDS 45.0%
GAGCAACGGAACAAAGGTTA+CGG - chr7_1:45558172-45558191 Msa1021250:CDS 45.0%
AGCTCCACACTTGTGTTGAA+AGG + chr7_1:45558203-45558222 None:intergenic 45.0%
AGTCATCCTCTCCATTTGCA+TGG - chr7_1:45558257-45558276 Msa1021250:CDS 45.0%
! AATACAATTGGTGTCCCGTC+TGG - chr7_1:45558371-45558390 Msa1021250:intron 45.0%
TCCTAGCAGATAATCCAGGT+TGG - chr7_1:45558414-45558433 Msa1021250:intron 45.0%
ACCAACCTGGATTATCTGCT+AGG + chr7_1:45558418-45558437 None:intergenic 45.0%
AAACTAGGAAGGAACCAACC+TGG + chr7_1:45558431-45558450 None:intergenic 45.0%
TAAGATGGCATGGATCGTCT+TGG - chr7_1:45558591-45558610 Msa1021250:CDS 45.0%
TAACACTTGGGAAGATCAGC+TGG + chr7_1:45558652-45558671 None:intergenic 45.0%
!! GGCCCTTTTTGTTATCCAAC+TGG - chr7_1:45558726-45558745 Msa1021250:CDS 45.0%
!!! ATTTTTTTATTTTTACATTT+TGG - chr7_1:45558705-45558724 Msa1021250:intron 5.0%
GCATTTGAGCTTGCAATGGC+CGG - chr7_1:45556157-45556176 Msa1021250:three_prime_UTR 50.0%
!! TTGGTAGTGTCTGGTAGTGC+CGG + chr7_1:45556179-45556198 None:intergenic 50.0%
CTGTCACCATCCTCTTGTTG+TGG + chr7_1:45556336-45556355 None:intergenic 50.0%
ACAGTGAACGGTCAGTTTCC+AGG - chr7_1:45556352-45556371 Msa1021250:CDS 50.0%
GTTTCCAGGACCTCGCATTA+TGG - chr7_1:45556366-45556385 Msa1021250:intron 50.0%
CCTTCTCTAGCCATAATGCG+AGG + chr7_1:45556379-45556398 None:intergenic 50.0%
CCTCGCATTATGGCTAGAGA+AGG - chr7_1:45556376-45556395 Msa1021250:intron 50.0%
!! GGGCATTGAGTCACATATGC+CGG + chr7_1:45556596-45556615 None:intergenic 50.0%
TTGCAGGGCTTGTGCTATGA+TGG + chr7_1:45556912-45556931 None:intergenic 50.0%
ATAGCACAAGCCCTGCAAAC+TGG - chr7_1:45556914-45556933 Msa1021250:CDS 50.0%
TAGCACAAGCCCTGCAAACT+GGG - chr7_1:45556915-45556934 Msa1021250:CDS 50.0%
GAGCAGTTATACAATGGCCC+TGG + chr7_1:45556983-45557002 None:intergenic 50.0%
!! CGTCGGCTTCAACAACTTTG+AGG + chr7_1:45557563-45557582 None:intergenic 50.0%
AACACCATCCTTATAGCCCC+TGG - chr7_1:45557609-45557628 Msa1021250:intron 50.0%
GTTGTCAAAAGTTCCCTGGC+CGG + chr7_1:45557715-45557734 None:intergenic 50.0%
TGGAACGTTAGCCGGAAACT+GGG + chr7_1:45557898-45557917 None:intergenic 50.0%
CATTGGGTCCCTGACAAGTT+TGG + chr7_1:45557983-45558002 None:intergenic 50.0%
CAAACTTGTCAGGGACCCAA+TGG - chr7_1:45557981-45558000 Msa1021250:CDS 50.0%
! GATTGAAGAAGTGCGGTGGT+TGG + chr7_1:45558050-45558069 None:intergenic 50.0%
! GTGTGATTGAAGAAGTGCGG+TGG + chr7_1:45558054-45558073 None:intergenic 50.0%
!! CACCATGGTGTTGTTTGGTG+GGG + chr7_1:45558153-45558172 None:intergenic 50.0%
!! CTCACCATGGTGTTGTTTGG+TGG + chr7_1:45558155-45558174 None:intergenic 50.0%
CTTTGTTCCGTTGCTCACCA+TGG + chr7_1:45558168-45558187 None:intergenic 50.0%
CATGGTGAGCAACGGAACAA+AGG - chr7_1:45558166-45558185 Msa1021250:CDS 50.0%
CTGCAAGACACTAGCATCCT+TGG - chr7_1:45558227-45558246 Msa1021250:CDS 50.0%
GAGGATGACTTTCAGCACCA+AGG + chr7_1:45558247-45558266 None:intergenic 50.0%
ATAGCAACCCATCCACCAGA+CGG + chr7_1:45558389-45558408 None:intergenic 50.0%
GTTGGGGTCTTAAGATGGCA+TGG - chr7_1:45558581-45558600 Msa1021250:CDS 50.0%
ATGGCATGGATCGTCTTGGA+TGG - chr7_1:45558595-45558614 Msa1021250:intron 50.0%
CTAGCCATAATGCGAGGTCC+TGG + chr7_1:45556373-45556392 None:intergenic 55.0%
!! CTTCAATCGGGATGGGCTGA+TGG - chr7_1:45556569-45556588 Msa1021250:CDS 55.0%
AGCACAAGCCCTGCAAACTG+GGG - chr7_1:45556916-45556935 Msa1021250:CDS 55.0%
TTGGCCAGGGGCTATAAGGA+TGG + chr7_1:45557616-45557635 None:intergenic 55.0%
! TGGTTTGGCCAGGGGCTATA+AGG + chr7_1:45557620-45557639 None:intergenic 55.0%
TAGACCATATGCTACCGGCC+AGG - chr7_1:45557698-45557717 Msa1021250:intron 55.0%
AGACCATATGCTACCGGCCA+GGG - chr7_1:45557699-45557718 Msa1021250:intron 55.0%
! ACTGGGCACTGCCAAGACTA+CGG + chr7_1:45557881-45557900 None:intergenic 55.0%
TCTTGGCAGTGCCCAGTTTC+CGG - chr7_1:45557884-45557903 Msa1021250:intron 55.0%
GTGGAACGTTAGCCGGAAAC+TGG + chr7_1:45557899-45557918 None:intergenic 55.0%
! ATAAGGGGCGTAGACACCCT+TGG + chr7_1:45558090-45558109 None:intergenic 55.0%
!! GGGGTGCCGGTGTAATTGAA+TGG + chr7_1:45558134-45558153 None:intergenic 55.0%
CACCCCACCAAACAACACCA+TGG - chr7_1:45558148-45558167 Msa1021250:CDS 55.0%
! ACAATTGGTGTCCCGTCTGG+TGG - chr7_1:45558374-45558393 Msa1021250:intron 55.0%
TAGCAACCCATCCACCAGAC+GGG + chr7_1:45558388-45558407 None:intergenic 55.0%
TTGGGAAGATCAGCTGGTGG+TGG + chr7_1:45558646-45558665 None:intergenic 55.0%
CACTTGGGAAGATCAGCTGG+TGG + chr7_1:45558649-45558668 None:intergenic 55.0%
!! ATCGGGATGGGCTGATGGAC+CGG - chr7_1:45556574-45556593 Msa1021250:CDS 60.0%
GCACAAGCCCTGCAAACTGG+GGG - chr7_1:45556917-45556936 Msa1021250:CDS 60.0%
TGGTCCTCCCCCAGTTTGCA+GGG + chr7_1:45556927-45556946 None:intergenic 60.0%
TTGGTCCTCCCCCAGTTTGC+AGG + chr7_1:45556928-45556947 None:intergenic 60.0%
GTTCCCTGGCCGGTAGCATA+TGG + chr7_1:45557705-45557724 None:intergenic 60.0%
!! GGTGTTGTTTGGTGGGGTGC+CGG + chr7_1:45558147-45558166 None:intergenic 60.0%
! TTGGTGTCCCGTCTGGTGGA+TGG - chr7_1:45558378-45558397 Msa1021250:intron 60.0%
! TGGTGTCCCGTCTGGTGGAT+GGG - chr7_1:45558379-45558398 Msa1021250:intron 60.0%
CAAGCCCTGCAAACTGGGGG+AGG - chr7_1:45556920-45556939 Msa1021250:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 45556005 45558911 45556005 ID=Msa1021250;Name=Msa1021250
chr7_1 mRNA 45556005 45558911 45556005 ID=Msa1021250-mRNA-1;Parent=Msa1021250;Name=Msa1021250-mRNA-1;_AED=0.08;_eAED=0.08;_QI=86|1|1|1|1|1|6|239|580
chr7_1 exon 45556005 45556374 45556005 ID=Msa1021250-mRNA-1:exon:8992;Parent=Msa1021250-mRNA-1
chr7_1 exon 45556485 45557456 45556485 ID=Msa1021250-mRNA-1:exon:8991;Parent=Msa1021250-mRNA-1
chr7_1 exon 45557906 45558034 45557906 ID=Msa1021250-mRNA-1:exon:8990;Parent=Msa1021250-mRNA-1
chr7_1 exon 45558119 45558363 45558119 ID=Msa1021250-mRNA-1:exon:8989;Parent=Msa1021250-mRNA-1
chr7_1 exon 45558461 45558612 45558461 ID=Msa1021250-mRNA-1:exon:8988;Parent=Msa1021250-mRNA-1
chr7_1 exon 45558712 45558911 45558712 ID=Msa1021250-mRNA-1:exon:8987;Parent=Msa1021250-mRNA-1
chr7_1 five_prime_UTR 45558826 45558911 45558826 ID=Msa1021250-mRNA-1:five_prime_utr;Parent=Msa1021250-mRNA-1
chr7_1 CDS 45558712 45558825 45558712 ID=Msa1021250-mRNA-1:cds;Parent=Msa1021250-mRNA-1
chr7_1 CDS 45558461 45558612 45558461 ID=Msa1021250-mRNA-1:cds;Parent=Msa1021250-mRNA-1
chr7_1 CDS 45558119 45558363 45558119 ID=Msa1021250-mRNA-1:cds;Parent=Msa1021250-mRNA-1
chr7_1 CDS 45557906 45558034 45557906 ID=Msa1021250-mRNA-1:cds;Parent=Msa1021250-mRNA-1
chr7_1 CDS 45556485 45557456 45556485 ID=Msa1021250-mRNA-1:cds;Parent=Msa1021250-mRNA-1
chr7_1 CDS 45556244 45556374 45556244 ID=Msa1021250-mRNA-1:cds;Parent=Msa1021250-mRNA-1
chr7_1 three_prime_UTR 45556005 45556243 45556005 ID=Msa1021250-mRNA-1:three_prime_utr;Parent=Msa1021250-mRNA-1
Gene Sequence

>Msa1021250

ATGGCTGTTACCATTTTTTCATCGCTTAAAATTGCAACGTTGTTTTTGTCATTGATAATTTTTTGCGCATTTGAGCTTGCAATGGCCGGCACTACCAGACACTACCAATTTGATATAGGGTACCAAAATGTGACAAGATTGTGCCACAACAAGAGGATGGTGACAGTGAACGGTCAGTTTCCAGGACCTCGCATTATGGCTAGAGAAGGAGATCGTCTTGTTATCAAAGTGGTGAACAATGTGCAAAACAACATCTCGATTCATTGGCATGGCATTCGACAACTTCAATCGGGATGGGCTGATGGACCGGCATATGTGACTCAATGCCCTATCCAAACAGGTCAAAGCTATGTTTACAATTACACAATTAAAGGCCAAAGAGGAACACTCTTTTGGCATGCTCACATATCTTGGTTAAGATCAACACTCTATGGTCCTCTCATCATTCTTCCCAAGAAAAATGTTCCATATCCTTTTGCAAAACCCCACAAGGAAGTTCCCATGATATTTGGTGAATGGTTCAATGCAGATACTGAAGCCATCATAGCACAAGCCCTGCAAACTGGGGGAGGACCAAATGTTTCCGAAGCCTACACAATTAATGGACTTCCAGGGCCATTGTATAACTGCTCTAAAAAAGATACATTCAAGCTAAAGGTCAAGCCTGGAAAGACTTACCTTCTACGTTTGATCAACGCTGCACTAAACGATGAGTTGTTCTTCAGCATTGCAAATCACACCCTCAAAGTTGTTGAAGCCGACGCTATTTATGTGAAACCTTTTGAGACTAACACCATCCTTATAGCCCCTGGCCAAACCACTAATGTTCTTCTCAAGACCAAGTCACACTATCCCAATGCAGCCTTCTTAATGAGTGCTAGACCATATGCTACCGGCCAGGGAACTTTTGACAACACAACTGTTGCTGGTATCTTGGAATATGAAATCCCATCAAAGACTCATCATTCCGCTTCTTCACTAAAAAAGCTTCCACTATTCAAACCTACTCTCCCAGCACTTAATGACACTTCTTTTGCAACAAAGTTTTCGAAGAAACTCCGTAGTCTTGGCAGTGCCCAGTTTCCGGCTAACGTTCCACAGAAAGTTGATAAGCACTTTTTCTTCACAGTAAGCCTTGGTACCAACCCTTGTCAAAGCAACCAAACTTGTCAGGGACCCAATGGAACAATGTTTGCAGCATCAGTGAACAACGTATCTTTCACAACGCCAACCACCGCACTTCTTCAATCACACTTTACTGGACAATCCAAGGGTGTCTACGCCCCTTATTTCCCAAACAGTCCCTTGCATCCATTCAATTACACCGGCACCCCACCAAACAACACCATGGTGAGCAACGGAACAAAGGTTACGGTTCTTCCTTTCAACACAAGTGTGGAGCTTGTGCTGCAAGACACTAGCATCCTTGGTGCTGAAAGTCATCCTCTCCATTTGCATGGCTTTAACTTCTTTGTTGTTGGACAAGGATTTGGTAACTACGATTCAAATAAGGACCCAAAAAACTTTAACCTTGTTGATCCTGTTGAAAGGAATACAATTGGTGTCCCGTCTGGTGGATGGGTTGCTATCAGATTCCTAGCAGATAATCCAGGAGTATGGTTCATGCATTGTCACTTGGAAGTACATACAAGTTGGGGTCTTAAGATGGCATGGATCGTCTTGGATGGAAAGCTTCCAAATCAAAAGTTACTTCCACCACCAGCTGATCTTCCCAAGTGTTAA
Protein sequence

>Msa1021250

MAVTIFSSLKIATLFLSLIIFCAFELAMAGTTRHYQFDIGYQNVTRLCHNKRMVTVNGQFPGPRIMAREGDRLVIKVVNNVQNNISIHWHGIRQLQSGWADGPAYVTQCPIQTGQSYVYNYTIKGQRGTLFWHAHISWLRSTLYGPLIILPKKNVPYPFAKPHKEVPMIFGEWFNADTEAIIAQALQTGGGPNVSEAYTINGLPGPLYNCSKKDTFKLKVKPGKTYLLRLINAALNDELFFSIANHTLKVVEADAIYVKPFETNTILIAPGQTTNVLLKTKSHYPNAAFLMSARPYATGQGTFDNTTVAGILEYEIPSKTHHSASSLKKLPLFKPTLPALNDTSFATKFSKKLRSLGSAQFPANVPQKVDKHFFFTVSLGTNPCQSNQTCQGPNGTMFAASVNNVSFTTPTTALLQSHFTGQSKGVYAPYFPNSPLHPFNYTGTPPNNTMVSNGTKVTVLPFNTSVELVLQDTSILGAESHPLHLHGFNFFVVGQGFGNYDSNKDPKNFNLVDPVERNTIGVPSGGWVAIRFLADNPGVWFMHCHLEVHTSWGLKMAWIVLDGKLPNQKLLPPPADLPKC*