Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1023730 | KEH17097.1 | 69.167 | 120 | 16 | 1 | 1 | 120 | 1 | 99 | 1.45e-48 | 163 |
| Msa1023730 | KEH17097.1 | 82.000 | 50 | 9 | 0 | 75 | 124 | 11 | 60 | 2.77e-20 | 91.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1023730 | G7ZUP9 | 69.167 | 120 | 16 | 1 | 1 | 120 | 1 | 99 | 6.94e-49 | 163 |
| Msa1023730 | G7ZUP9 | 82.000 | 50 | 9 | 0 | 75 | 124 | 11 | 60 | 1.32e-20 | 91.7 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1023730 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1023730 | Msa1400560 | 0.880268 | 6.311678e-70 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAGCCATATTTCAGTTTA+TGG | 0.073373 | 7_1:+51906586 | Msa1023730:CDS |
| GTGCTTTGAACGTCCATAAA+AGG | 0.285913 | 7_1:+51906406 | Msa1023730:CDS |
| GTGCTTTGAACGTCCATAAA+AGG | 0.292733 | 7_1:+51908655 | Msa1023730:CDS |
| CTATACCATAAACTGAAATA+TGG | 0.300254 | 7_1:-51906591 | None:intergenic |
| ATATACTATAAACTGAAATA+TGG | 0.301575 | 7_1:-51908840 | None:intergenic |
| TGTTCTCCAGTATATCTTCT+TGG | 0.311912 | 7_1:-51906566 | None:intergenic |
| TGTTCTCCAGTATATCTTCT+TGG | 0.311912 | 7_1:-51908815 | None:intergenic |
| AATTCTCCAAGAAGATATAC+TGG | 0.369151 | 7_1:+51906560 | Msa1023730:CDS |
| AATTCTCCAAGAAGATATAC+TGG | 0.369151 | 7_1:+51908809 | Msa1023730:CDS |
| TCCCAAGTCCTTCGAAAGTA+AGG | 0.396127 | 7_1:+51906383 | Msa1023730:CDS |
| TCCCAAGTCCTTCGAAAGTA+GGG | 0.449688 | 7_1:+51908632 | Msa1023730:intron |
| GTGGTTGACATGTCTTCATA+TGG | 0.455172 | 7_1:-51906500 | None:intergenic |
| GTGGTTGACATGTCTTCATA+TGG | 0.455172 | 7_1:-51908749 | None:intergenic |
| ACCCTTACTTTCGAAGGACT+TGG | 0.470340 | 7_1:-51906385 | None:intergenic |
| CCCTTACTTTCGAAGGACTT+GGG | 0.477508 | 7_1:-51906384 | None:intergenic |
| CAAAGCACCCTTACTTTCGA+AGG | 0.524991 | 7_1:-51906391 | None:intergenic |
| GTCCCAAGTCCTTCGAAAGT+AGG | 0.556450 | 7_1:+51908631 | Msa1023730:intron |
| TTATGTACTTGCGGCTTCTG+TGG | 0.572456 | 7_1:-51906519 | None:intergenic |
| TTATGTACTTGCGGCTTCTG+TGG | 0.572456 | 7_1:-51908768 | None:intergenic |
| CAAAGCACCCCTACTTTCGA+AGG | 0.573103 | 7_1:-51908640 | None:intergenic |
| CCCAAGTCCTTCGAAAGTAA+GGG | 0.608380 | 7_1:+51906384 | Msa1023730:CDS |
| CCCAAGTCCTTCGAAAGTAG+GGG | 0.658544 | 7_1:+51908633 | Msa1023730:intron |
| TGGGACAATCAAAGCAAGCG+TGG | 0.684170 | 7_1:-51906365 | None:intergenic |
| AAAACACGTTGCGATAGATG+TGG | 0.685090 | 7_1:+51906444 | Msa1023730:CDS |
| AAAACACGTTGCGATAGATG+TGG | 0.685090 | 7_1:+51908693 | Msa1023730:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATATATATATTTAATAA+GGG | - | chr7_1:51907981-51908000 | None:intergenic | 0.0% |
| !! | ATATATATATATATTTAATA+AGG | - | chr7_1:51907980-51907999 | None:intergenic | 0.0% |
| !!! | TTAATTTTAGTTAAATTTCT+TGG | + | chr7_1:51906743-51906762 | Msa1023730:intron | 10.0% |
| !! | ATAAAATTATAAACTCAAAA+TGG | + | chr7_1:51907077-51907096 | Msa1023730:intron | 10.0% |
| !!! | TTTCTATTTAGAATATTTTT+AGG | + | chr7_1:51907257-51907276 | Msa1023730:intron | 10.0% |
| !! | GTTATTTATAAAAAAAAATG+AGG | + | chr7_1:51907349-51907368 | Msa1023730:intron | 10.0% |
| !! | AATTCTAATAAATCATATTT+AGG | + | chr7_1:51908054-51908073 | Msa1023730:intron | 10.0% |
| !! | ATTCTAATAAATCATATTTA+GGG | + | chr7_1:51908055-51908074 | Msa1023730:intron | 10.0% |
| !! | ATTCTATTAAATCATATTTA+GGG | + | chr7_1:51908163-51908182 | Msa1023730:intron | 10.0% |
| !!! | ATGAGTATTTTTTTTCAAAA+GGG | - | chr7_1:51907190-51907209 | None:intergenic | 15.0% |
| !!! | TATGAGTATTTTTTTTCAAA+AGG | - | chr7_1:51907191-51907210 | None:intergenic | 15.0% |
| !!! | TCTTTATTTATTTTCAATTG+AGG | + | chr7_1:51907286-51907305 | Msa1023730:intron | 15.0% |
| !!! | CTTTATTTATTTTCAATTGA+GGG | + | chr7_1:51907287-51907306 | Msa1023730:intron | 15.0% |
| !! | GATTCTATTAAATCATATTT+AGG | + | chr7_1:51908162-51908181 | Msa1023730:intron | 15.0% |
| !! | ATATACTATAAACTGAAATA+TGG | - | chr7_1:51908843-51908862 | None:intergenic | 15.0% |
| !!! | TTTTAATTATGTATGAGAGT+AGG | + | chr7_1:51906785-51906804 | Msa1023730:intron | 20.0% |
| !! | TGTGAATAATAAACTACAAA+AGG | - | chr7_1:51906887-51906906 | None:intergenic | 20.0% |
| !!! | TATATGTGATGCTAAATTTT+AGG | - | chr7_1:51907921-51907940 | None:intergenic | 20.0% |
| ! | AGAATTCTTCAAATTCATTG+AGG | - | chr7_1:51906546-51906565 | None:intergenic | 25.0% |
| ! | CTATACCATAAACTGAAATA+TGG | - | chr7_1:51906594-51906613 | None:intergenic | 25.0% |
| ! | CATATTTCAGTTTATGGTAT+AGG | + | chr7_1:51906592-51906611 | Msa1023730:intron | 25.0% |
| !! | TTTGTCGACTATATATTCTT+AGG | + | chr7_1:51906662-51906681 | Msa1023730:intron | 25.0% |
| ! | TACTATAATTCTTGCATGTA+TGG | + | chr7_1:51906699-51906718 | Msa1023730:intron | 25.0% |
| ! | AGGATGAAATATTCTATTAC+AGG | + | chr7_1:51906805-51906824 | Msa1023730:intron | 25.0% |
| ! | AACATAATTAAAAGCTTACG+AGG | + | chr7_1:51906988-51907007 | Msa1023730:intron | 25.0% |
| ! | AATTCCATTGTATACTTGTA+GGG | + | chr7_1:51907851-51907870 | Msa1023730:intron | 25.0% |
| ! | GCAACAAAAACTATGAAATA+AGG | + | chr7_1:51908403-51908422 | Msa1023730:intron | 25.0% |
| ! | TTATTCAACAGTATATCCAT+GGG | - | chr7_1:51908465-51908484 | None:intergenic | 25.0% |
| ! | ATTATTCAACAGTATATCCA+TGG | - | chr7_1:51908466-51908485 | None:intergenic | 25.0% |
| ! | AGAATTCTTCAAATTCATTG+AGG | - | chr7_1:51906546-51906565 | None:intergenic | 25.0% |
| ! | CTTCACATTCAAACCTTTTA+TGG | - | chr7_1:51906422-51906441 | None:intergenic | 30.0% |
| AATTCTCCAAGAAGATATAC+TGG | + | chr7_1:51906560-51906579 | Msa1023730:CDS | 30.0% | |
| ACAAGCCATATTTCAGTTTA+TGG | + | chr7_1:51906586-51906605 | Msa1023730:CDS | 30.0% | |
| TAGTTTATTATTCACAGCTC+CGG | + | chr7_1:51906891-51906910 | Msa1023730:intron | 30.0% | |
| !!! | TTTTGATGTCATTTTGCTCT+TGG | - | chr7_1:51907467-51907486 | None:intergenic | 30.0% |
| ATTGACAACATATTAGGGTA+GGG | - | chr7_1:51907515-51907534 | None:intergenic | 30.0% | |
| AATTGACAACATATTAGGGT+AGG | - | chr7_1:51907516-51907535 | None:intergenic | 30.0% | |
| GAGCAATTGACAACATATTA+GGG | - | chr7_1:51907520-51907539 | None:intergenic | 30.0% | |
| TGAGCAATTGACAACATATT+AGG | - | chr7_1:51907521-51907540 | None:intergenic | 30.0% | |
| ! | AAATTAGTTGACTCTTGAAC+TGG | - | chr7_1:51907822-51907841 | None:intergenic | 30.0% |
| CTACAAGTATACAATGGAAT+TGG | - | chr7_1:51907852-51907871 | None:intergenic | 30.0% | |
| CAATTCCATTGTATACTTGT+AGG | + | chr7_1:51907850-51907869 | Msa1023730:intron | 30.0% | |
| !!! | AGCTAAGTTTTGGGTTTAAA+TGG | - | chr7_1:51907880-51907899 | None:intergenic | 30.0% |
| !! | TGTGATGCTAAATTTTAGGT+GGG | - | chr7_1:51907917-51907936 | None:intergenic | 30.0% |
| ! | ATGTGATGCTAAATTTTAGG+TGG | - | chr7_1:51907918-51907937 | None:intergenic | 30.0% |
| !! | TAAATTTTGTTGTGAACCCA+TGG | + | chr7_1:51908446-51908465 | Msa1023730:intron | 30.0% |
| ! | CTTCACATTCAAACCTTTTA+TGG | - | chr7_1:51906422-51906441 | None:intergenic | 30.0% |
| AATTCTCCAAGAAGATATAC+TGG | + | chr7_1:51906560-51906579 | Msa1023730:CDS | 30.0% | |
| CATTGAGGATTATGTACTTG+CGG | - | chr7_1:51906531-51906550 | None:intergenic | 35.0% | |
| TGTTCTCCAGTATATCTTCT+TGG | - | chr7_1:51906569-51906588 | None:intergenic | 35.0% | |
| ! | TCCATACAGAGCTAAGTTTT+GGG | - | chr7_1:51907889-51907908 | None:intergenic | 35.0% |
| AAATAGTGCATTAGAGTGTG+AGG | - | chr7_1:51908527-51908546 | None:intergenic | 35.0% | |
| CATTGAGGATTATGTACTTG+CGG | - | chr7_1:51906531-51906550 | None:intergenic | 35.0% | |
| TGTTCTCCAGTATATCTTCT+TGG | - | chr7_1:51906569-51906588 | None:intergenic | 35.0% | |
| ! | GTGCTTTGAACGTCCATAAA+AGG | + | chr7_1:51906406-51906425 | Msa1023730:CDS | 40.0% |
| AAAACACGTTGCGATAGATG+TGG | + | chr7_1:51906444-51906463 | Msa1023730:CDS | 40.0% | |
| GTGGTTGACATGTCTTCATA+TGG | - | chr7_1:51906503-51906522 | None:intergenic | 40.0% | |
| GAAGCATGAACGAACTTCAT+AGG | + | chr7_1:51906961-51906980 | Msa1023730:intron | 40.0% | |
| ACGAGGATTAAAAGCTTACG+AGG | + | chr7_1:51907005-51907024 | Msa1023730:intron | 40.0% | |
| GAAAACCATTGAGTCTGCTA+GGG | - | chr7_1:51907048-51907067 | None:intergenic | 40.0% | |
| TGAAAACCATTGAGTCTGCT+AGG | - | chr7_1:51907049-51907068 | None:intergenic | 40.0% | |
| AGAATAGTGTCCAACTACGT+TGG | + | chr7_1:51907487-51907506 | Msa1023730:intron | 40.0% | |
| GAATAGTGTCCAACTACGTT+GGG | + | chr7_1:51907488-51907507 | Msa1023730:intron | 40.0% | |
| GAGACCCTACAAGTATACAA+TGG | - | chr7_1:51907858-51907877 | None:intergenic | 40.0% | |
| !! | ACCCAAAACTTAGCTCTGTA+TGG | + | chr7_1:51907885-51907904 | Msa1023730:intron | 40.0% |
| ! | GTCCATACAGAGCTAAGTTT+TGG | - | chr7_1:51907890-51907909 | None:intergenic | 40.0% |
| GAACATCGTGGATGATTCTT+AGG | - | chr7_1:51908595-51908614 | None:intergenic | 40.0% | |
| ! | GTGCTTTGAACGTCCATAAA+AGG | + | chr7_1:51906406-51906425 | Msa1023730:CDS | 40.0% |
| AAAACACGTTGCGATAGATG+TGG | + | chr7_1:51906444-51906463 | Msa1023730:CDS | 40.0% | |
| GTGGTTGACATGTCTTCATA+TGG | - | chr7_1:51906503-51906522 | None:intergenic | 40.0% | |
| TCCCAAGTCCTTCGAAAGTA+AGG | + | chr7_1:51906383-51906402 | Msa1023730:CDS | 45.0% | |
| CCCTTACTTTCGAAGGACTT+GGG | - | chr7_1:51906387-51906406 | None:intergenic | 45.0% | |
| CCCAAGTCCTTCGAAAGTAA+GGG | + | chr7_1:51906384-51906403 | Msa1023730:CDS | 45.0% | |
| ACCCTTACTTTCGAAGGACT+TGG | - | chr7_1:51906388-51906407 | None:intergenic | 45.0% | |
| ! | CAAAGCACCCTTACTTTCGA+AGG | - | chr7_1:51906394-51906413 | None:intergenic | 45.0% |
| TTATGTACTTGCGGCTTCTG+TGG | - | chr7_1:51906522-51906541 | None:intergenic | 45.0% | |
| CCGAATACATCACACATGAC+CGG | - | chr7_1:51906913-51906932 | None:intergenic | 45.0% | |
| CCGGTCATGTGTGATGTATT+CGG | + | chr7_1:51906910-51906929 | Msa1023730:intron | 45.0% | |
| ACAAACCCTAGCAGACTCAA+TGG | + | chr7_1:51907040-51907059 | Msa1023730:intron | 45.0% | |
| AGTGCATTAGAGTGTGAGGT+AGG | - | chr7_1:51908523-51908542 | None:intergenic | 45.0% | |
| TCCCAAGTCCTTCGAAAGTA+GGG | + | chr7_1:51906383-51906402 | Msa1023730:CDS | 45.0% | |
| TTATGTACTTGCGGCTTCTG+TGG | - | chr7_1:51906522-51906541 | None:intergenic | 45.0% | |
| !!! | TTATTAATTGAATATTTTAA+TGG | + | chr7_1:51907109-51907128 | Msa1023730:intron | 5.0% |
| !! | TTATTTATAAAAAAAAATGA+GGG | + | chr7_1:51907350-51907369 | Msa1023730:intron | 5.0% |
| !!! | ATTTATTTATTTTTATTTGA+AGG | + | chr7_1:51907640-51907659 | Msa1023730:intron | 5.0% |
| !! | TTATTAAAAAAAAAAAAACT+TGG | + | chr7_1:51907793-51907812 | Msa1023730:intron | 5.0% |
| TGGGACAATCAAAGCAAGCG+TGG | - | chr7_1:51906368-51906387 | None:intergenic | 50.0% | |
| AAAGCAAGCGTGGAACATCG+TGG | - | chr7_1:51908607-51908626 | None:intergenic | 50.0% | |
| TGGGACAATCAAAGCAAGCG+TGG | - | chr7_1:51906368-51906387 | None:intergenic | 50.0% | |
| GTCCCAAGTCCTTCGAAAGT+AGG | + | chr7_1:51906382-51906401 | Msa1023730:CDS | 50.0% | |
| CCCCTACTTTCGAAGGACTT+GGG | - | chr7_1:51908636-51908655 | None:intergenic | 50.0% | |
| CCCAAGTCCTTCGAAAGTAG+GGG | + | chr7_1:51908633-51908652 | Msa1023730:intron | 50.0% | |
| ACCCCTACTTTCGAAGGACT+TGG | - | chr7_1:51908637-51908656 | None:intergenic | 50.0% | |
| ! | CAAAGCACCCCTACTTTCGA+AGG | - | chr7_1:51908643-51908662 | None:intergenic | 50.0% |
| GGGTAGGGTCCCAACGTAGT+TGG | - | chr7_1:51907500-51907519 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 51906360 | 51908910 | 51906360 | ID=Msa1023730;Name=Msa1023730 |
| chr7_1 | mRNA | 51906360 | 51908910 | 51906360 | ID=Msa1023730-mRNA-1;Parent=Msa1023730;Name=Msa1023730-mRNA-1;_AED=0.35;_eAED=0.54;_QI=0|0|0|1|0|0|4|0|155 |
| chr7_1 | exon | 51906360 | 51906533 | 51906360 | ID=Msa1023730-mRNA-1:exon:10246;Parent=Msa1023730-mRNA-1 |
| chr7_1 | exon | 51906560 | 51906607 | 51906560 | ID=Msa1023730-mRNA-1:exon:10247;Parent=Msa1023730-mRNA-1 |
| chr7_1 | exon | 51908639 | 51908782 | 51908639 | ID=Msa1023730-mRNA-1:exon:10248;Parent=Msa1023730-mRNA-1 |
| chr7_1 | exon | 51908809 | 51908910 | 51908809 | ID=Msa1023730-mRNA-1:exon:10249;Parent=Msa1023730-mRNA-1 |
| chr7_1 | CDS | 51906360 | 51906533 | 51906360 | ID=Msa1023730-mRNA-1:cds;Parent=Msa1023730-mRNA-1 |
| chr7_1 | CDS | 51906560 | 51906607 | 51906560 | ID=Msa1023730-mRNA-1:cds;Parent=Msa1023730-mRNA-1 |
| chr7_1 | CDS | 51908639 | 51908782 | 51908639 | ID=Msa1023730-mRNA-1:cds;Parent=Msa1023730-mRNA-1 |
| chr7_1 | CDS | 51908809 | 51908910 | 51908809 | ID=Msa1023730-mRNA-1:cds;Parent=Msa1023730-mRNA-1 |
| Gene Sequence |
| Protein sequence |
