Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0380200 | Msa1024440 | 0.899574 | 1.731239e-77 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1024440 | MtrunA17_Chr5g0396051 | 80.000 | 30 | 6 | 0 | 50 | 79 | 20 | 49 | 1.49e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTTATTCTCGGGCGATTT+TGG | 0.124814 | 7_1:-53782218 | Msa1024440:CDS |
| ACCAAAATCGCCCGAGAATA+AGG | 0.245936 | 7_1:+53782217 | None:intergenic |
| CAGTTTCAGTTTGGTATGTT+TGG | 0.315862 | 7_1:-53782315 | Msa1024440:CDS |
| GCGCAATAGATGTACAAAAT+AGG | 0.330846 | 7_1:+53782166 | None:intergenic |
| GAGATTATATTAGCACTAAC+TGG | 0.370425 | 7_1:-53782745 | Msa1024440:intron |
| ATGTACAAAATAGGCATAAC+AGG | 0.372999 | 7_1:+53782175 | None:intergenic |
| ATTTGGTTATGCCTTATTCT+CGG | 0.381342 | 7_1:-53782228 | Msa1024440:CDS |
| TTGTACATCTATTGCGCTGC+AGG | 0.405137 | 7_1:-53782160 | Msa1024440:CDS |
| AATATAATCTCAACTCCATT+TGG | 0.411567 | 7_1:+53782757 | None:intergenic |
| TTTGGTTATGCCTTATTCTC+GGG | 0.463573 | 7_1:-53782227 | Msa1024440:CDS |
| GTACTGCCACAGTTTCAGTT+TGG | 0.481719 | 7_1:-53782324 | Msa1024440:intron |
| AACTCCTCCGGCTGATGTAG+TGG | 0.529536 | 7_1:+53782118 | None:intergenic |
| AAAAGGATGAAAGAGCCAAA+TGG | 0.543139 | 7_1:-53782772 | None:intergenic |
| TCAGTTGCCACTACATCAGC+CGG | 0.560737 | 7_1:-53782125 | Msa1024440:CDS |
| ATTCAAGCTCGCAACTCCTC+CGG | 0.563890 | 7_1:+53782106 | None:intergenic |
| AAATAGGCATAACAGGTGCA+CGG | 0.668392 | 7_1:+53782182 | None:intergenic |
| GTTGCCACTACATCAGCCGG+AGG | 0.709608 | 7_1:-53782122 | Msa1024440:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAAATATGGTTA+TGG | - | chr7_1:53782159-53782178 | Msa1024440:CDS | 10.0% |
| !! | GTAAGCAAAAAAAAAAAATA+TGG | - | chr7_1:53782153-53782172 | Msa1024440:CDS | 15.0% |
| !!! | ATTTTTGTATTATGCATATT+TGG | - | chr7_1:53782629-53782648 | Msa1024440:intron | 15.0% |
| !! | AAAAAAATGCTCATTCATAT+TGG | + | chr7_1:53782336-53782355 | None:intergenic | 20.0% |
| ! | AATATAATCTCAACTCCATT+TGG | + | chr7_1:53782120-53782139 | None:intergenic | 25.0% |
| !! | GTTGTTGTTGTTAATCAAAT+TGG | - | chr7_1:53782220-53782239 | Msa1024440:CDS | 25.0% |
| !! | TTTTCCAACCATATTGAATT+AGG | + | chr7_1:53782293-53782312 | None:intergenic | 25.0% |
| !!! | TGTTTTTTATGACTGTTTAG+TGG | - | chr7_1:53782474-53782493 | Msa1024440:intron | 25.0% |
| GAGATTATATTAGCACTAAC+TGG | - | chr7_1:53782129-53782148 | Msa1024440:CDS | 30.0% | |
| AACAACAACTAAAGCTATTG+TGG | + | chr7_1:53782209-53782228 | None:intergenic | 30.0% | |
| ! | GTTGTTGTTAATCAAATTGG+TGG | - | chr7_1:53782223-53782242 | Msa1024440:CDS | 30.0% |
| ! | CATTCATATTGGTCTAACAT+TGG | + | chr7_1:53782325-53782344 | None:intergenic | 30.0% |
| TTGGCTATTAAATTCTACAG+TGG | - | chr7_1:53782441-53782460 | Msa1024440:intron | 30.0% | |
| ! | AAGTGGGTTTCATTAACTTA+AGG | - | chr7_1:53782499-53782518 | Msa1024440:intron | 30.0% |
| ! | AGTGGGTTTCATTAACTTAA+GGG | - | chr7_1:53782500-53782519 | Msa1024440:intron | 30.0% |
| ! | TTGGCTATTAAATTTTGCTG+CGG | - | chr7_1:53782578-53782597 | Msa1024440:intron | 30.0% |
| ! | ATTTGGTTATGCCTTATTCT+CGG | - | chr7_1:53782646-53782665 | Msa1024440:intron | 30.0% |
| ATGTACAAAATAGGCATAAC+AGG | + | chr7_1:53782702-53782721 | None:intergenic | 30.0% | |
| GTTGTTAATCAAATTGGTGG+TGG | - | chr7_1:53782226-53782245 | Msa1024440:CDS | 35.0% | |
| TCTAAGGGCCTAATTCAATA+TGG | - | chr7_1:53782282-53782301 | Msa1024440:CDS | 35.0% | |
| ! | TTTTTGTACTGCCTTTGCTT+TGG | - | chr7_1:53782408-53782427 | Msa1024440:intron | 35.0% |
| AATATACCAAGCCAAAGCAA+AGG | + | chr7_1:53782422-53782441 | None:intergenic | 35.0% | |
| !! | TTGCTTTGGCTTGGTATATT+TGG | - | chr7_1:53782422-53782441 | Msa1024440:intron | 35.0% |
| GCTATTAAATTCTACAGTGG+TGG | - | chr7_1:53782444-53782463 | Msa1024440:intron | 35.0% | |
| ATGACTGTTTAGTGGAAAAG+TGG | - | chr7_1:53782482-53782501 | Msa1024440:intron | 35.0% | |
| TGACTGTTTAGTGGAAAAGT+GGG | - | chr7_1:53782483-53782502 | Msa1024440:intron | 35.0% | |
| !! | TTCATTAACTTAAGGGTGTC+TGG | - | chr7_1:53782507-53782526 | Msa1024440:intron | 35.0% |
| AACATACCAAACTGAAACTG+TGG | + | chr7_1:53782559-53782578 | None:intergenic | 35.0% | |
| CAGTTTCAGTTTGGTATGTT+TGG | - | chr7_1:53782559-53782578 | Msa1024440:intron | 35.0% | |
| ! | TTTGGTTATGCCTTATTCTC+GGG | - | chr7_1:53782647-53782666 | Msa1024440:intron | 35.0% |
| GCGCAATAGATGTACAAAAT+AGG | + | chr7_1:53782711-53782730 | None:intergenic | 35.0% | |
| TCGCAAACCATGAAGATCTA+AGG | - | chr7_1:53782266-53782285 | Msa1024440:CDS | 40.0% | |
| CGCAAACCATGAAGATCTAA+GGG | - | chr7_1:53782267-53782286 | Msa1024440:CDS | 40.0% | |
| ATTAGGCCCTTAGATCTTCA+TGG | + | chr7_1:53782276-53782295 | None:intergenic | 40.0% | |
| AGGGCCTAATTCAATATGGT+TGG | - | chr7_1:53782286-53782305 | Msa1024440:CDS | 40.0% | |
| TAAATTCTACAGTGGTGGCA+TGG | - | chr7_1:53782449-53782468 | Msa1024440:intron | 40.0% | |
| ! | GCTATTAAATTTTGCTGCGG+TGG | - | chr7_1:53782581-53782600 | Msa1024440:intron | 40.0% |
| AAATAGGCATAACAGGTGCA+CGG | + | chr7_1:53782695-53782714 | None:intergenic | 40.0% | |
| GTACTGCCACAGTTTCAGTT+TGG | - | chr7_1:53782550-53782569 | Msa1024440:intron | 45.0% | |
| ACCAAAATCGCCCGAGAATA+AGG | + | chr7_1:53782660-53782679 | None:intergenic | 45.0% | |
| TTGTACATCTATTGCGCTGC+AGG | - | chr7_1:53782714-53782733 | Msa1024440:intron | 45.0% | |
| !! | ATGGCATGTGCTACTTCAGC+AGG | - | chr7_1:53782178-53782197 | Msa1024440:CDS | 50.0% |
| GTACTGCCTTTGCTTTGGCT+TGG | - | chr7_1:53782413-53782432 | Msa1024440:intron | 50.0% | |
| !! | GCCTTATTCTCGGGCGATTT+TGG | - | chr7_1:53782656-53782675 | Msa1024440:intron | 50.0% |
| TCAGTTGCCACTACATCAGC+CGG | - | chr7_1:53782749-53782768 | Msa1024440:CDS | 50.0% | |
| AACTCCTCCGGCTGATGTAG+TGG | + | chr7_1:53782759-53782778 | None:intergenic | 55.0% | |
| GTTGCCACTACATCAGCCGG+AGG | - | chr7_1:53782752-53782771 | Msa1024440:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 53782108 | 53782788 | 53782108 | ID=Msa1024440;Name=Msa1024440 |
| chr7_1 | mRNA | 53782108 | 53782788 | 53782108 | ID=Msa1024440-mRNA-1;Parent=Msa1024440;Name=Msa1024440-mRNA-1;_AED=0.47;_eAED=0.74;_QI=0|0|0|1|0|0|2|0|89 |
| chr7_1 | exon | 53782746 | 53782788 | 53782746 | ID=Msa1024440-mRNA-1:exon:10643;Parent=Msa1024440-mRNA-1 |
| chr7_1 | exon | 53782108 | 53782334 | 53782108 | ID=Msa1024440-mRNA-1:exon:10642;Parent=Msa1024440-mRNA-1 |
| chr7_1 | CDS | 53782746 | 53782788 | 53782746 | ID=Msa1024440-mRNA-1:cds;Parent=Msa1024440-mRNA-1 |
| chr7_1 | CDS | 53782108 | 53782334 | 53782108 | ID=Msa1024440-mRNA-1:cds;Parent=Msa1024440-mRNA-1 |
| Gene Sequence |
| Protein sequence |
