Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0237660 | Msa0380200 | 0.806712 | 7.201228e-50 | -8.615850e-47 |
| Msa0380190 | Msa0380200 | 0.807581 | 4.713578e-50 | -8.615850e-47 |
| Msa0380200 | Msa1024440 | 0.899574 | 1.731239e-77 | -8.615850e-47 |
| Msa0380200 | Msa1024450 | 0.806981 | 6.317802e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0380200 | MtrunA17_Chr5g0396051 | 80.000 | 30 | 6 | 0 | 50 | 79 | 20 | 49 | 1.49e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTTATTCTCGGGCGATTT+TGG | 0.124814 | 3_1:+81854824 | Msa0380200:CDS |
| ACCAAAATCGCCCGAGAATA+AGG | 0.245936 | 3_1:-81854825 | None:intergenic |
| CAGTTTCAGTTTGGTATGTT+TGG | 0.315862 | 3_1:+81854727 | Msa0380200:intron |
| GCGCAATAGATGTACAAAAT+AGG | 0.330846 | 3_1:-81854876 | None:intergenic |
| GAGATTATATTAGCACTAAC+TGG | 0.370425 | 3_1:+81854297 | Msa0380200:CDS |
| ATGTACAAAATAGGCATAAC+AGG | 0.372999 | 3_1:-81854867 | None:intergenic |
| ATTTGGTTATGCCTTATTCT+CGG | 0.381342 | 3_1:+81854814 | Msa0380200:CDS |
| TTGTACATCTATTGCGCTGC+AGG | 0.405137 | 3_1:+81854882 | Msa0380200:CDS |
| AATATAATCTCAACTCCATT+TGG | 0.411567 | 3_1:-81854285 | None:intergenic |
| TTTGGTTATGCCTTATTCTC+GGG | 0.463573 | 3_1:+81854815 | Msa0380200:CDS |
| GTACTGCCACAGTTTCAGTT+TGG | 0.481719 | 3_1:+81854718 | Msa0380200:intron |
| AACTCCTCCGGCTGATGTAG+TGG | 0.529536 | 3_1:-81854924 | None:intergenic |
| AAAAGGATGAAAGAGCCAAA+TGG | 0.543139 | 3_1:+81854270 | None:intergenic |
| TCAGTTGCCACTACATCAGC+CGG | 0.560737 | 3_1:+81854917 | Msa0380200:CDS |
| ATTCAAGCTCGCAACTCCTC+CGG | 0.563890 | 3_1:-81854936 | None:intergenic |
| AAATAGGCATAACAGGTGCA+CGG | 0.668392 | 3_1:-81854860 | None:intergenic |
| GTTGCCACTACATCAGCCGG+AGG | 0.709608 | 3_1:+81854920 | Msa0380200:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAAATATGGTTA+TGG | + | chr3_1:81854327-81854346 | Msa0380200:intron | 10.0% |
| !! | GTAAGCAAAAAAAAAAAATA+TGG | + | chr3_1:81854321-81854340 | Msa0380200:intron | 15.0% |
| !!! | ATTTTTGTATTATGCATATT+TGG | + | chr3_1:81854797-81854816 | Msa0380200:CDS | 15.0% |
| !! | AAAAAAATGCTCATTCATAT+TGG | - | chr3_1:81854504-81854523 | None:intergenic | 20.0% |
| ! | AATATAATCTCAACTCCATT+TGG | - | chr3_1:81854288-81854307 | None:intergenic | 25.0% |
| !! | GTTGTTGTTGTTAATCAAAT+TGG | + | chr3_1:81854388-81854407 | Msa0380200:intron | 25.0% |
| !! | TTTTCCAACCATATTGAATT+AGG | - | chr3_1:81854461-81854480 | None:intergenic | 25.0% |
| !!! | TGTTTTTTATGACTGTTTAG+TGG | + | chr3_1:81854642-81854661 | Msa0380200:intron | 25.0% |
| GAGATTATATTAGCACTAAC+TGG | + | chr3_1:81854297-81854316 | Msa0380200:CDS | 30.0% | |
| AACAACAACTAAAGCTATTG+TGG | - | chr3_1:81854377-81854396 | None:intergenic | 30.0% | |
| ! | GTTGTTGTTAATCAAATTGG+TGG | + | chr3_1:81854391-81854410 | Msa0380200:intron | 30.0% |
| ! | CATTCATATTGGTCTAACAT+TGG | - | chr3_1:81854493-81854512 | None:intergenic | 30.0% |
| TTGGCTATTAAATTCTACAG+TGG | + | chr3_1:81854609-81854628 | Msa0380200:intron | 30.0% | |
| ! | AAGTGGGTTTCATTAACTTA+AGG | + | chr3_1:81854667-81854686 | Msa0380200:intron | 30.0% |
| ! | AGTGGGTTTCATTAACTTAA+GGG | + | chr3_1:81854668-81854687 | Msa0380200:intron | 30.0% |
| ! | TTGGCTATTAAATTTTGCTG+CGG | + | chr3_1:81854746-81854765 | Msa0380200:CDS | 30.0% |
| ! | ATTTGGTTATGCCTTATTCT+CGG | + | chr3_1:81854814-81854833 | Msa0380200:CDS | 30.0% |
| ATGTACAAAATAGGCATAAC+AGG | - | chr3_1:81854870-81854889 | None:intergenic | 30.0% | |
| GTTGTTAATCAAATTGGTGG+TGG | + | chr3_1:81854394-81854413 | Msa0380200:intron | 35.0% | |
| TCTAAGGGCCTAATTCAATA+TGG | + | chr3_1:81854450-81854469 | Msa0380200:intron | 35.0% | |
| ! | TTTTTGTACTGCCTTTGCTT+TGG | + | chr3_1:81854576-81854595 | Msa0380200:intron | 35.0% |
| AATATACCAAGCCAAAGCAA+AGG | - | chr3_1:81854590-81854609 | None:intergenic | 35.0% | |
| !! | TTGCTTTGGCTTGGTATATT+TGG | + | chr3_1:81854590-81854609 | Msa0380200:intron | 35.0% |
| GCTATTAAATTCTACAGTGG+TGG | + | chr3_1:81854612-81854631 | Msa0380200:intron | 35.0% | |
| ATGACTGTTTAGTGGAAAAG+TGG | + | chr3_1:81854650-81854669 | Msa0380200:intron | 35.0% | |
| TGACTGTTTAGTGGAAAAGT+GGG | + | chr3_1:81854651-81854670 | Msa0380200:intron | 35.0% | |
| !! | TTCATTAACTTAAGGGTGTC+TGG | + | chr3_1:81854675-81854694 | Msa0380200:intron | 35.0% |
| AACATACCAAACTGAAACTG+TGG | - | chr3_1:81854727-81854746 | None:intergenic | 35.0% | |
| CAGTTTCAGTTTGGTATGTT+TGG | + | chr3_1:81854727-81854746 | Msa0380200:intron | 35.0% | |
| ! | TTTGGTTATGCCTTATTCTC+GGG | + | chr3_1:81854815-81854834 | Msa0380200:CDS | 35.0% |
| GCGCAATAGATGTACAAAAT+AGG | - | chr3_1:81854879-81854898 | None:intergenic | 35.0% | |
| TCGCAAACCATGAAGATCTA+AGG | + | chr3_1:81854434-81854453 | Msa0380200:intron | 40.0% | |
| CGCAAACCATGAAGATCTAA+GGG | + | chr3_1:81854435-81854454 | Msa0380200:intron | 40.0% | |
| ATTAGGCCCTTAGATCTTCA+TGG | - | chr3_1:81854444-81854463 | None:intergenic | 40.0% | |
| AGGGCCTAATTCAATATGGT+TGG | + | chr3_1:81854454-81854473 | Msa0380200:intron | 40.0% | |
| TAAATTCTACAGTGGTGGCA+TGG | + | chr3_1:81854617-81854636 | Msa0380200:intron | 40.0% | |
| ! | GCTATTAAATTTTGCTGCGG+TGG | + | chr3_1:81854749-81854768 | Msa0380200:CDS | 40.0% |
| AAATAGGCATAACAGGTGCA+CGG | - | chr3_1:81854863-81854882 | None:intergenic | 40.0% | |
| GTACTGCCACAGTTTCAGTT+TGG | + | chr3_1:81854718-81854737 | Msa0380200:intron | 45.0% | |
| ACCAAAATCGCCCGAGAATA+AGG | - | chr3_1:81854828-81854847 | None:intergenic | 45.0% | |
| TTGTACATCTATTGCGCTGC+AGG | + | chr3_1:81854882-81854901 | Msa0380200:CDS | 45.0% | |
| !! | ATGGCATGTGCTACTTCAGC+AGG | + | chr3_1:81854346-81854365 | Msa0380200:intron | 50.0% |
| GTACTGCCTTTGCTTTGGCT+TGG | + | chr3_1:81854581-81854600 | Msa0380200:intron | 50.0% | |
| !! | GCCTTATTCTCGGGCGATTT+TGG | + | chr3_1:81854824-81854843 | Msa0380200:CDS | 50.0% |
| TCAGTTGCCACTACATCAGC+CGG | + | chr3_1:81854917-81854936 | Msa0380200:CDS | 50.0% | |
| AACTCCTCCGGCTGATGTAG+TGG | - | chr3_1:81854927-81854946 | None:intergenic | 55.0% | |
| GTTGCCACTACATCAGCCGG+AGG | + | chr3_1:81854920-81854939 | Msa0380200:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 81854276 | 81854956 | 81854276 | ID=Msa0380200;Name=Msa0380200 |
| chr3_1 | mRNA | 81854276 | 81854956 | 81854276 | ID=Msa0380200-mRNA-1;Parent=Msa0380200;Name=Msa0380200-mRNA-1;_AED=0.47;_eAED=0.74;_QI=0|0|0|1|0|0|2|0|89 |
| chr3_1 | exon | 81854276 | 81854318 | 81854276 | ID=Msa0380200-mRNA-1:exon:20827;Parent=Msa0380200-mRNA-1 |
| chr3_1 | exon | 81854730 | 81854956 | 81854730 | ID=Msa0380200-mRNA-1:exon:20828;Parent=Msa0380200-mRNA-1 |
| chr3_1 | CDS | 81854276 | 81854318 | 81854276 | ID=Msa0380200-mRNA-1:cds;Parent=Msa0380200-mRNA-1 |
| chr3_1 | CDS | 81854730 | 81854956 | 81854730 | ID=Msa0380200-mRNA-1:cds;Parent=Msa0380200-mRNA-1 |
| Gene Sequence |
| Protein sequence |
