Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1029890 | XP_003624327.1 | 78.302 | 1060 | 66 | 10 | 1 | 896 | 1 | 1060 | 0.0 | 1564 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1029890 | sp|Q8VZG8|MIK2_ARATH | 37.992 | 1016 | 467 | 23 | 7 | 868 | 31 | 1037 | 0.0 | 576 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0463270 | Msa1029890 | 0.803305 | 3.714377e-49 | -8.615850e-47 |
| Msa1029890 | Msa1068810 | 0.853047 | 3.092665e-61 | -8.615850e-47 |
| Msa1029890 | Msa1120930 | 0.853200 | 2.796960e-61 | -8.615850e-47 |
| Msa1029890 | Msa1156750 | 0.825588 | 4.318613e-54 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1029890 | MtrunA17_Chr7g0250471 | 78.302 | 1060 | 66 | 10 | 1 | 896 | 1 | 1060 | 0.0 | 1564 |
| Msa1029890 | MtrunA17_Chr7g0250381 | 63.405 | 1063 | 217 | 14 | 1 | 894 | 1 | 1060 | 0.0 | 1206 |
| Msa1029890 | MtrunA17_Chr7g0250351 | 67.268 | 776 | 163 | 10 | 209 | 894 | 79 | 853 | 0.0 | 968 |
| Msa1029890 | MtrunA17_Chr7g0250491 | 52.538 | 1064 | 327 | 16 | 9 | 894 | 14 | 1077 | 0.0 | 957 |
| Msa1029890 | MtrunA17_Chr5g0439291 | 46.318 | 1032 | 394 | 19 | 3 | 895 | 22 | 1032 | 0.0 | 752 |
| Msa1029890 | MtrunA17_Chr1g0165951 | 45.000 | 1000 | 419 | 20 | 1 | 873 | 4 | 999 | 0.0 | 735 |
| Msa1029890 | MtrunA17_Chr7g0233881 | 46.604 | 957 | 379 | 17 | 26 | 890 | 44 | 960 | 0.0 | 733 |
| Msa1029890 | MtrunA17_Chr1g0165971 | 42.490 | 1052 | 421 | 24 | 1 | 873 | 8 | 1054 | 0.0 | 705 |
| Msa1029890 | MtrunA17_Chr7g0232451 | 45.587 | 963 | 397 | 21 | 26 | 875 | 51 | 999 | 0.0 | 692 |
| Msa1029890 | MtrunA17_Chr7g0232631 | 44.917 | 964 | 424 | 17 | 15 | 875 | 32 | 991 | 0.0 | 691 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1029890 | AT4G08850.1 | 37.992 | 1016 | 467 | 23 | 7 | 868 | 31 | 1037 | 0.0 | 576 |
| Msa1029890 | AT1G35710.1 | 37.613 | 888 | 436 | 17 | 83 | 868 | 241 | 1112 | 6.80e-172 | 529 |
| Msa1029890 | AT1G35710.1 | 32.841 | 542 | 259 | 11 | 31 | 540 | 36 | 504 | 2.92e-59 | 220 |
| Msa1029890 | AT4G08850.2 | 36.688 | 924 | 427 | 21 | 7 | 777 | 31 | 949 | 3.51e-158 | 490 |
| Msa1029890 | AT1G17230.1 | 30.783 | 1085 | 526 | 26 | 7 | 879 | 5 | 1076 | 2.11e-128 | 414 |
| Msa1029890 | AT1G17230.2 | 30.734 | 1077 | 537 | 24 | 7 | 879 | 5 | 1076 | 4.63e-128 | 414 |
| Msa1029890 | AT3G24240.1 | 33.145 | 884 | 467 | 20 | 88 | 884 | 226 | 1072 | 5.71e-121 | 395 |
| Msa1029890 | AT3G24240.1 | 35.728 | 529 | 284 | 12 | 50 | 529 | 61 | 582 | 2.11e-70 | 254 |
| Msa1029890 | AT1G28440.1 | 31.313 | 990 | 494 | 29 | 41 | 890 | 31 | 974 | 1.54e-119 | 387 |
| Msa1029890 | AT5G63930.2 | 32.704 | 954 | 482 | 22 | 87 | 887 | 80 | 1026 | 2.36e-119 | 388 |
Find 163 sgRNAs with CRISPR-Local
Find 254 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTGTTCTAGCATCCGA+AGG | 0.649589 | 7_1:+63458211 | Msa1029890:CDS |
| AAAATGAGAACTTAGAATGT+TGG | 0.521945 | 7_1:-63459433 | None:intergenic |
| AAACCTTTGTGTTATTAGAA+AGG | 0.404788 | 7_1:-63457560 | None:intergenic |
| AAACTTAGCGATCCCGAAGT+CGG | 0.561778 | 7_1:-63459669 | None:intergenic |
| AAATGTGATGTATACAGTTT+TGG | 0.382117 | 7_1:+63459921 | Msa1029890:CDS |
| AACGGAGCAAGAAGAAAAGC+TGG | 0.510900 | 7_1:-63458915 | None:intergenic |
| AACTCATTGAAATACTCTAT+TGG | 0.332158 | 7_1:+63460249 | Msa1029890:exon |
| AAGAAGTTGTGGAGCTACCG+AGG | 0.645767 | 7_1:+63458634 | Msa1029890:CDS |
| AAGATAACTTCCTCATCAAT+TGG | 0.389635 | 7_1:-63460083 | None:intergenic |
| AAGATGAAGCCTACCTAGCT+TGG | 0.503384 | 7_1:-63458436 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCCAAAACTTTCAAGGTTTA+TGG | - | chr7_1:63457046-63457065 | None:intergenic | 30.0% | |
| GTAATGTGAACATTTCTTGA+GGG | - | chr7_1:63457230-63457249 | None:intergenic | 30.0% | |
| TGTAATGTGAACATTTCTTG+AGG | - | chr7_1:63457231-63457250 | None:intergenic | 30.0% | |
| ATTCCAAACTGTAACCTTAT+TGG | + | chr7_1:63457417-63457436 | Msa1029890:CDS | 30.0% | |
| GGTTCTATTCCAAAAGAAAT+TGG | + | chr7_1:63457438-63457457 | Msa1029890:CDS | 30.0% | |
| TTGTCAACAAATTCTCTATC+TGG | + | chr7_1:63457489-63457508 | Msa1029890:CDS | 30.0% | |
| TAATTTACTCATGTTGCCTA+TGG | - | chr7_1:63457529-63457548 | None:intergenic | 30.0% | |
| TCTAATAACACAAAGGTTTC+TGG | + | chr7_1:63457564-63457583 | Msa1029890:intron | 30.0% | |
| TTCAAATTTCCAATTGTAGG+TGG | - | chr7_1:63457985-63458004 | None:intergenic | 30.0% | |
| AGCTTCAAATTTCCAATTGT+AGG | - | chr7_1:63457988-63458007 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 63456829 | 63460483 | 63456829 | ID=Msa1029890;Name=Msa1029890 |
| chr7_1 | mRNA | 63456829 | 63460483 | 63456829 | ID=Msa1029890-mRNA-1;Parent=Msa1029890;Name=Msa1029890-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0.15|0.14|1|0.07|0|14|183|896 |
| chr7_1 | exon | 63456829 | 63457057 | 63456829 | ID=Msa1029890-mRNA-1:exon:13807;Parent=Msa1029890-mRNA-1 |
| chr7_1 | exon | 63457073 | 63457252 | 63457073 | ID=Msa1029890-mRNA-1:exon:13808;Parent=Msa1029890-mRNA-1 |
| chr7_1 | exon | 63457346 | 63457578 | 63457346 | ID=Msa1029890-mRNA-1:exon:13809;Parent=Msa1029890-mRNA-1 |
| chr7_1 | exon | 63457802 | 63458045 | 63457802 | ID=Msa1029890-mRNA-1:exon:13810;Parent=Msa1029890-mRNA-1 |
| chr7_1 | exon | 63458050 | 63458095 | 63458050 | ID=Msa1029890-mRNA-1:exon:13811;Parent=Msa1029890-mRNA-1 |
| chr7_1 | exon | 63458158 | 63458405 | 63458158 | ID=Msa1029890-mRNA-1:exon:13812;Parent=Msa1029890-mRNA-1 |
| chr7_1 | exon | 63458421 | 63458655 | 63458421 | ID=Msa1029890-mRNA-1:exon:13813;Parent=Msa1029890-mRNA-1 |
| chr7_1 | exon | 63458680 | 63458863 | 63458680 | ID=Msa1029890-mRNA-1:exon:13814;Parent=Msa1029890-mRNA-1 |
| chr7_1 | exon | 63458891 | 63459072 | 63458891 | ID=Msa1029890-mRNA-1:exon:13815;Parent=Msa1029890-mRNA-1 |
| chr7_1 | exon | 63459112 | 63459271 | 63459112 | ID=Msa1029890-mRNA-1:exon:13816;Parent=Msa1029890-mRNA-1 |
| chr7_1 | exon | 63459281 | 63459476 | 63459281 | ID=Msa1029890-mRNA-1:exon:13817;Parent=Msa1029890-mRNA-1 |
| chr7_1 | exon | 63459558 | 63459752 | 63459558 | ID=Msa1029890-mRNA-1:exon:13818;Parent=Msa1029890-mRNA-1 |
| chr7_1 | exon | 63459892 | 63460403 | 63459892 | ID=Msa1029890-mRNA-1:exon:13819;Parent=Msa1029890-mRNA-1 |
| chr7_1 | exon | 63460454 | 63460483 | 63460454 | ID=Msa1029890-mRNA-1:exon:13820;Parent=Msa1029890-mRNA-1 |
| chr7_1 | CDS | 63456829 | 63457057 | 63456829 | ID=Msa1029890-mRNA-1:cds;Parent=Msa1029890-mRNA-1 |
| chr7_1 | CDS | 63457073 | 63457252 | 63457073 | ID=Msa1029890-mRNA-1:cds;Parent=Msa1029890-mRNA-1 |
| chr7_1 | CDS | 63457346 | 63457578 | 63457346 | ID=Msa1029890-mRNA-1:cds;Parent=Msa1029890-mRNA-1 |
| chr7_1 | CDS | 63457802 | 63458045 | 63457802 | ID=Msa1029890-mRNA-1:cds;Parent=Msa1029890-mRNA-1 |
| chr7_1 | CDS | 63458050 | 63458095 | 63458050 | ID=Msa1029890-mRNA-1:cds;Parent=Msa1029890-mRNA-1 |
| chr7_1 | CDS | 63458158 | 63458405 | 63458158 | ID=Msa1029890-mRNA-1:cds;Parent=Msa1029890-mRNA-1 |
| chr7_1 | CDS | 63458421 | 63458655 | 63458421 | ID=Msa1029890-mRNA-1:cds;Parent=Msa1029890-mRNA-1 |
| chr7_1 | CDS | 63458680 | 63458863 | 63458680 | ID=Msa1029890-mRNA-1:cds;Parent=Msa1029890-mRNA-1 |
| chr7_1 | CDS | 63458891 | 63459072 | 63458891 | ID=Msa1029890-mRNA-1:cds;Parent=Msa1029890-mRNA-1 |
| chr7_1 | CDS | 63459112 | 63459271 | 63459112 | ID=Msa1029890-mRNA-1:cds;Parent=Msa1029890-mRNA-1 |
| chr7_1 | CDS | 63459281 | 63459476 | 63459281 | ID=Msa1029890-mRNA-1:cds;Parent=Msa1029890-mRNA-1 |
| chr7_1 | CDS | 63459558 | 63459752 | 63459558 | ID=Msa1029890-mRNA-1:cds;Parent=Msa1029890-mRNA-1 |
| chr7_1 | CDS | 63459892 | 63460250 | 63459892 | ID=Msa1029890-mRNA-1:cds;Parent=Msa1029890-mRNA-1 |
| chr7_1 | three_prime_UTR | 63460251 | 63460403 | 63460251 | ID=Msa1029890-mRNA-1:three_prime_utr;Parent=Msa1029890-mRNA-1 |
| chr7_1 | three_prime_UTR | 63460454 | 63460483 | 63460454 | ID=Msa1029890-mRNA-1:three_prime_utr;Parent=Msa1029890-mRNA-1 |
| Gene Sequence |
>Msa1029890 ATGGCACTATCCACATTCATCATGATTCTGTTCATAATTTTGTTCACTTCATGGCCACAAGCTGTAGCTCAAGACAGTGAAGCAAAATCAGCTCTATTAAAATGGAAAAATAGCTTTGACAACCAAAGCCAAGCTCTCTTGCCAACTTGGAAAAATACCACAAATCCATGCAGATGGCAAGGAATTCACTGTGACAAGTCCAATTCAATTACTACCATAAACCTTGAAAGTACACTTCACTCTCTCACATTTTCTTCATTCACAAACCTCTCAACCCTAAATATCTATAATAACCACTTTTATGGAACCATTCCTCCACAAATTGGTAACATCTCAAAAATAAATACATTGAATTTTTCTCTAAATCCTATTGATGGTCCCATCCCTCAAGAAATGTTCACATTACAAAGAGGCAACAATTTTTTTGGCACTCCTATTCCTCCTGAGATTGGAAAATTGAACAAATTATGGTTTCTATCAATTCCAAACTGTAACCTTATTGGTTCTATTCCAAAAGAAATTGGGTTTTTGACAAACCTTACTTATATTGATTTGTCAACAAATTCTCTATCTGGTTTTATCCCTGAAACCATAGGCAACATGAGTAAATTAAATTTACTTATCCTTTCTAATAACACAAAGCTTGCACTTGATCACAACCGCCTTTCTGGAACCATTCCTTCCACAATTGGAAACTTGAAAAGTCTCACACGTTTGTTTTTGGGATTCAATCGTTTTTCTGGATCAATTCCTGCCTCTATTGGAAATTTGATCAATTTGGATATCCTTAGTCTCCAAGCAAACAATCTCACTGGAACAATTCCACCTACAATTGGAAATTTGAAGCTGCTTCGTGTCTTTGAACTATCTACCAATAAACTTCATGAATTCCAAATGAATTCAATAACAATACCAATTGGTATTCCTTTGTTGTTCTTAAATGCTGATAATAACCGTTTCACTGGTCCAATACCAACAACTTTGAAAAACTGTTCTAGCATCCGAAGGATCAGAATTGAGGCAAATCAAATAGAAGGTGATATAGCACAAGATTTTGGTGTGTATCCAAATTTGCAATACTTTGAGGCGAGTGATAATAAATTCCATGGACAAATTTCACCAAACTGGGGAAAGTGTCTTAATCTTCAGAACTTTATAATATCTAACAATAATATTTCTGAACTTACTCGTTTGACCAAGCTAGGTAGGCTTCATCTTTATTCAAATCAATTGACTGGGAAACTTCCAAAGGAATTAGGAAACATGACATCATTAATGGAACTCAAGATTAGTAACAATCATTTCTCGGAAAACATCCCAACAGAAATTGGATCGCTACAAACACTTAACGAGTTGGACCTCGGAGGAAACGAGTTGAGTGGCACGATACCGAAAGAAGTTGTGGAGCTACCGAGAAACAAAATTGAAGGAAATATTCCATCTCTGTTTGGTTCAGCTCTTGAGTCACTTGATCTTAGTGGCAATCTTTTGAATGGAACAATACCAACAGCTCTTGAAGACTTGCTGCAGTTGTCAATGTTGAATCTCTCACACAATATGCTTTCAGGAACCATTCCTCAAAATTTTGAAAGGAATTTGTTAGAAGGCCCACTTCCTAAAATTCCAGCTTTTCTTCTTGCTCCGTTTGAATCATTAAAAAATAACAAAGGCTTATGCGGAAATATCACAGGTTTGGTTCCTTGTCCAACAAACAATAGTCGAAAGAGAAAAAATGTCATTCGATCGGTATTCATTGCTTTAGGAGCTCTAATATTAGTGTTAAAACCACGAAAGGAAAAAAGCCAAACGGATGAAAAAGCACAAAGGGGAGTACTATTTTCAAATTGGAGTCATGATGGGAAAATGACGTTTGAAAGTATTATTCAAGCTACTGAGAATTTTGATGACAAATATCTCATTGGAGTTGGAAGTCAAGGAAATGCAGAGCTGTCCAGCGGTTCGGTTGGTGCCATATATGCTGTGAAGAAGCTTCATTTAGTAACAGATGATGAAATGTCAAAGTCTTTTACGAGTGAGATTGAAACATTGAGAGGAATCAAACACAGGAACATCATCAACCTTCAAGGATATTGCCAACATTCTAAGTTCTCATTTTTGGTTTACAAGTTCATGGAAGGTGTCGCCAATGCTTTGTCCTACTTGCATCACGATTGTTCACCTCCTATTGTTCATCGTGATATATCGAGTAAAAATGTTCTTATCAATCTTGACTATGAAGCTCATGTCTCCGACTTCGGGATCGCTAAGTTTCTAAAGCCTGACGAAACTAATCGGACCCAATTTGCAGGAACTCTGGGGTATGCAGCTCCAGAGTTGGCACAAACAATGAAAGTGAATGAGAAATGTGATGTATACAGTTTTGGAGTACTTGCTTTGGAAATCATAATGGGAGAACATCCTGGAGATCTCATTTCATTATATTTGTCACCATCTACAAGGCCATTGGCTAATGATACATTATTGACAGATGTTTTAGATCAAAAACCTCAAAAAGTTATGAAACCAATTGATGAGGAAGTTATCTTGATTGCAAAGTTAGCATTTTCTTGCATAAATCCAGAGCCACGCTCTCGGCCAACCATGGATCAAGTATGTAAGATGCTTGGAGCAGGAAAATCACCTTTGGAAGATCAAATTCACACGATCAAACTAGGACAACTTCTTCATTAA |
| Protein sequence |
>Msa1029890 MALSTFIMILFIILFTSWPQAVAQDSEAKSALLKWKNSFDNQSQALLPTWKNTTNPCRWQGIHCDKSNSITTINLESTLHSLTFSSFTNLSTLNIYNNHFYGTIPPQIGNISKINTLNFSLNPIDGPIPQEMFTLQRGNNFFGTPIPPEIGKLNKLWFLSIPNCNLIGSIPKEIGFLTNLTYIDLSTNSLSGFIPETIGNMSKLNLLILSNNTKLALDHNRLSGTIPSTIGNLKSLTRLFLGFNRFSGSIPASIGNLINLDILSLQANNLTGTIPPTIGNLKLLRVFELSTNKLHEFQMNSITIPIGIPLLFLNADNNRFTGPIPTTLKNCSSIRRIRIEANQIEGDIAQDFGVYPNLQYFEASDNKFHGQISPNWGKCLNLQNFIISNNNISELTRLTKLGRLHLYSNQLTGKLPKELGNMTSLMELKISNNHFSENIPTEIGSLQTLNELDLGGNELSGTIPKEVVELPRNKIEGNIPSLFGSALESLDLSGNLLNGTIPTALEDLLQLSMLNLSHNMLSGTIPQNFERNLLEGPLPKIPAFLLAPFESLKNNKGLCGNITGLVPCPTNNSRKRKNVIRSVFIALGALILVLKPRKEKSQTDEKAQRGVLFSNWSHDGKMTFESIIQATENFDDKYLIGVGSQGNAELSSGSVGAIYAVKKLHLVTDDEMSKSFTSEIETLRGIKHRNIINLQGYCQHSKFSFLVYKFMEGVANALSYLHHDCSPPIVHRDISSKNVLINLDYEAHVSDFGIAKFLKPDETNRTQFAGTLGYAAPELAQTMKVNEKCDVYSFGVLALEIIMGEHPGDLISLYLSPSTRPLANDTLLTDVLDQKPQKVMKPIDEEVILIAKLAFSCINPEPRSRPTMDQVCKMLGAGKSPLEDQIHTIKLGQLLH* |
