AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1030550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1030550 MtrunA17_Chr7g0251651 93.878 196 10 2 1 196 1 194 5.13e-137 380
Msa1030550 MtrunA17_Chr7g0251671 71.429 140 39 1 18 157 10 148 5.45e-72 215
Msa1030550 MtrunA17_Chr7g0251661 69.014 142 43 1 16 157 8 148 4.91e-71 212
Msa1030550 MtrunA17_Chr2g0309891 32.911 158 98 5 16 167 4 159 5.20e-21 88.6
Msa1030550 MtrunA17_Chr6g0481091 33.117 154 96 5 16 164 4 155 1.70e-20 85.5
Msa1030550 MtrunA17_Chr4g0047841 34.932 146 84 6 18 157 15 155 3.15e-19 84.0
Msa1030550 MtrunA17_Chr5g0442771 37.778 135 75 6 13 142 14 144 4.51e-19 83.2
Msa1030550 MtrunA17_Chr2g0311651 31.383 188 93 9 13 174 18 195 6.51e-19 82.4
Msa1030550 MtrunA17_Chr2g0311661 36.364 143 74 7 10 142 14 149 2.91e-18 80.5
Msa1030550 MtrunA17_Chr5g0433781 35.606 132 76 5 16 142 4 131 3.01e-18 82.0
Msa1030550 MtrunA17_Chr3g0109821 34.848 132 77 5 16 142 4 131 5.00e-18 81.3
Msa1030550 MtrunA17_Chr6g0453131 31.507 146 94 4 5 147 2 144 1.29e-17 79.7
Msa1030550 MtrunA17_Chr5g0417791 29.193 161 105 6 16 173 1 155 1.92e-17 77.8
Msa1030550 MtrunA17_Chr8g0384721 30.216 139 89 5 12 147 1 134 4.38e-17 77.4
Msa1030550 MtrunA17_Chr5g0417001 33.094 139 85 6 14 148 11 145 5.55e-17 77.8
Msa1030550 MtrunA17_Chr7g0252971 31.618 136 79 5 18 142 8 140 8.80e-17 77.0
Msa1030550 MtrunA17_Chr3g0130731 31.655 139 87 5 12 147 1 134 1.00e-16 76.3
Msa1030550 MtrunA17_Chr3g0124761 31.852 135 84 4 16 147 5 134 1.05e-16 76.3
Msa1030550 MtrunA17_Chr1g0147421 33.333 132 80 6 18 144 27 155 1.28e-16 76.6
Msa1030550 MtrunA17_Chr5g0428991 29.744 195 122 6 5 186 12 204 1.60e-16 77.0
Msa1030550 MtrunA17_Chr2g0318101 34.351 131 75 6 18 142 15 140 1.65e-16 76.3
Msa1030550 MtrunA17_Chr4g0042561 28.571 161 104 7 17 173 8 161 1.83e-16 75.5
Msa1030550 MtrunA17_Chr1g0201021 30.601 183 109 7 2 177 3 174 2.49e-16 76.3
Msa1030550 MtrunA17_Chr6g0461661 32.824 131 81 5 16 142 16 143 2.70e-16 75.9
Msa1030550 MtrunA17_Chr1g0183741 32.353 136 85 5 15 146 7 139 4.22e-16 74.3
Msa1030550 MtrunA17_Chr8g0345711 32.308 130 81 4 18 142 12 139 5.65e-16 72.0
Msa1030550 MtrunA17_Chr7g0214071 35.338 133 76 6 16 144 13 139 8.62e-16 73.2
Msa1030550 MtrunA17_Chr4g0018391 28.750 160 108 5 18 173 7 164 1.24e-15 73.9
Msa1030550 MtrunA17_Chr2g0316971 32.593 135 84 6 18 148 23 154 1.60e-15 73.6
Msa1030550 MtrunA17_Chr6g0453451 34.932 146 87 7 1 142 2 143 1.63e-15 73.2
Msa1030550 MtrunA17_Chr8g0345671 32.143 140 87 5 18 152 11 147 1.71e-15 73.6
Msa1030550 MtrunA17_Chr3g0139331 32.061 131 82 6 18 144 11 138 1.92e-15 73.2
Msa1030550 MtrunA17_Chr8g0361731 29.348 184 110 9 4 172 2 180 2.41e-15 73.2
Msa1030550 MtrunA17_Chr3g0128151 33.571 140 82 5 12 142 10 147 2.80e-15 73.6
Msa1030550 MtrunA17_Chr8g0345701 28.000 150 85 4 12 142 1 146 7.39e-15 69.3
Msa1030550 MtrunA17_Chr6g0484441 28.571 154 103 6 18 168 5 154 1.16e-14 70.9
Msa1030550 MtrunA17_Chr7g0261411 32.353 136 85 5 15 146 7 139 1.64e-14 70.1
Msa1030550 MtrunA17_Chr4g0060931 30.827 133 85 6 13 142 12 140 1.86e-14 70.5
Msa1030550 MtrunA17_Chr3g0144271 33.846 130 78 7 18 142 23 149 2.04e-14 70.9
Msa1030550 MtrunA17_Chr4g0054791 27.485 171 118 3 14 179 2 171 2.13e-14 70.1
Msa1030550 MtrunA17_Chr1g0197361 30.464 151 94 7 21 164 11 157 2.73e-14 70.5
Msa1030550 MtrunA17_Chr3g0091921 31.579 133 84 6 13 142 11 139 2.76e-14 69.7
Msa1030550 MtrunA17_Chr7g0263931 29.050 179 104 9 7 177 6 169 3.64e-14 69.7
Msa1030550 MtrunA17_Chr8g0345691 31.343 134 77 5 18 142 12 139 3.83e-14 70.1
Msa1030550 MtrunA17_Chr7g0253011 33.094 139 84 6 16 148 15 150 4.16e-14 69.7
Msa1030550 MtrunA17_Chr8g0359921 32.824 131 79 6 16 142 12 137 4.41e-14 69.3
Msa1030550 MtrunA17_Chr8g0372121 30.597 134 87 5 18 147 6 137 5.14e-14 68.9
Msa1030550 MtrunA17_Chr4g0056541 28.571 161 105 7 18 172 7 163 5.48e-14 69.3
Msa1030550 MtrunA17_Chr2g0326361 28.859 149 97 6 18 162 14 157 8.71e-14 68.6
Msa1030550 MtrunA17_Chr5g0400461 29.240 171 101 7 15 174 3 164 1.50e-13 67.8
Msa1030550 MtrunA17_Chr1g0197351 33.077 130 78 5 18 142 42 167 1.64e-13 68.2
Msa1030550 MtrunA17_Chr5g0398971 27.820 133 91 4 18 146 7 138 1.90e-13 67.8
Msa1030550 MtrunA17_Chr3g0130101 31.200 125 81 4 21 142 10 132 2.38e-13 67.4
Msa1030550 MtrunA17_Chr3g0128111 32.000 125 80 4 21 142 45 167 2.71e-13 66.2
Msa1030550 MtrunA17_Chr2g0318081 31.538 130 82 5 16 142 14 139 3.46e-13 67.0
Msa1030550 MtrunA17_Chr5g0405211 30.233 129 84 5 18 142 8 134 9.86e-13 65.5
Msa1030550 MtrunA17_Chr3g0092671 28.188 149 100 5 21 164 8 154 1.20e-12 65.9
Msa1030550 MtrunA17_Chr2g0309331 30.075 133 88 4 16 144 8 139 1.48e-12 65.1
Msa1030550 MtrunA17_Chr1g0200561 28.477 151 102 5 18 164 19 167 3.25e-12 64.3
Msa1030550 MtrunA17_Chr8g0385991 28.395 162 101 8 17 169 13 168 3.78e-12 62.8
Msa1030550 MtrunA17_Chr4g0076731 29.167 168 107 7 21 183 57 217 7.06e-12 63.5
Msa1030550 MtrunA17_Chr4g0017921 32.824 131 82 6 18 144 8 136 1.85e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1030550 AT3G10500.1 34.868 152 92 6 18 164 9 158 9.93e-22 92.4
Msa1030550 AT5G13180.1 36.957 138 77 5 9 142 5 136 2.80e-21 88.2
Msa1030550 AT5G04410.1 35.156 128 78 4 18 142 9 134 1.26e-20 89.4
Msa1030550 AT3G03200.1 32.927 164 102 5 16 173 4 165 2.57e-20 88.2
Msa1030550 AT5G17260.1 31.707 164 104 5 16 173 4 165 5.50e-20 87.0
Msa1030550 AT3G17730.1 35.659 129 80 3 16 142 4 131 7.93e-20 84.3
Msa1030550 AT1G54330.1 33.537 164 99 5 16 174 4 162 2.17e-19 84.0
Msa1030550 AT1G65910.1 32.099 162 102 6 16 172 4 162 4.69e-19 84.7
Msa1030550 AT5G39820.1 33.108 148 91 6 4 147 7 150 7.18e-19 83.2
Msa1030550 AT2G24430.2 34.884 129 77 6 18 142 16 141 9.96e-19 82.4
Msa1030550 AT2G24430.1 34.884 129 77 6 18 142 16 141 9.96e-19 82.4
Msa1030550 AT3G12910.1 36.879 141 77 6 13 146 17 152 1.00e-18 82.4
Msa1030550 AT1G76420.1 33.108 148 90 7 1 142 3 147 1.93e-18 82.0
Msa1030550 AT1G61110.1 34.615 130 75 5 18 142 16 140 6.00e-18 80.5
Msa1030550 AT3G04070.2 30.822 146 91 5 18 156 10 152 7.80e-18 80.5
Msa1030550 AT1G01720.1 30.256 195 114 8 16 194 5 193 9.78e-18 79.3
Msa1030550 AT2G33480.1 34.043 141 83 5 5 142 2 135 1.10e-17 79.0
Msa1030550 AT2G33480.2 34.043 141 83 5 5 142 2 135 1.18e-17 79.0
Msa1030550 AT3G04070.1 30.822 146 91 5 18 156 10 152 1.28e-17 79.7
Msa1030550 AT5G14000.1 27.746 173 115 5 5 173 2 168 2.83e-17 76.6
Msa1030550 AT5G07680.1 30.481 187 119 9 13 193 12 193 2.99e-17 78.6
Msa1030550 AT5G07680.2 30.978 184 116 9 16 193 1 179 3.30e-17 78.2
Msa1030550 AT5G08790.1 30.496 141 90 5 16 153 5 140 4.06e-17 77.8
Msa1030550 AT1G77450.1 32.593 135 83 4 16 147 8 137 4.62e-17 77.0
Msa1030550 AT1G26870.1 35.556 135 78 7 12 142 12 141 6.63e-17 78.2
Msa1030550 AT2G43000.1 29.381 194 114 9 13 191 14 199 7.43e-17 77.0
Msa1030550 AT5G18270.1 35.338 133 80 5 13 142 16 145 8.03e-17 77.4
Msa1030550 AT5G63790.2 31.111 135 85 5 16 147 58 187 1.12e-16 77.0
Msa1030550 AT5G63790.1 31.111 135 85 5 16 147 48 177 1.12e-16 77.0
Msa1030550 AT3G10490.1 36.434 129 75 6 18 142 27 152 1.21e-16 75.9
Msa1030550 AT5G18270.2 34.586 133 81 5 13 142 16 145 2.11e-16 76.3
Msa1030550 AT3G15170.1 32.593 135 84 6 12 142 14 145 2.89e-16 75.9
Msa1030550 AT3G10480.2 36.434 129 75 6 18 142 27 152 3.68e-16 76.3
Msa1030550 AT5G53950.1 33.083 133 82 6 14 142 13 142 3.99e-16 75.9
Msa1030550 AT3G10480.1 36.434 129 75 6 18 142 27 152 4.02e-16 76.3
Msa1030550 AT5G61430.1 33.835 133 81 6 13 142 11 139 5.33e-16 75.1
Msa1030550 AT2G27300.1 32.394 142 89 7 6 144 3 140 6.85e-16 74.7
Msa1030550 AT1G34180.1 33.071 127 80 4 21 144 19 143 9.25e-16 75.1
Msa1030550 AT1G34190.1 33.858 127 79 4 21 144 19 143 1.01e-15 75.1
Msa1030550 AT3G10490.2 36.434 129 75 6 18 142 27 152 1.26e-15 74.7
Msa1030550 AT5G14000.2 28.387 155 101 5 5 155 2 150 1.27e-15 71.2
Msa1030550 AT3G29035.1 31.522 184 104 9 16 193 22 189 1.44e-15 73.9
Msa1030550 AT1G34180.3 33.071 127 80 4 21 144 19 143 1.64e-15 74.3
Msa1030550 AT3G15510.1 33.600 125 76 5 22 142 21 142 1.87e-15 73.9
Msa1030550 AT5G39610.1 32.414 145 83 7 1 142 11 143 2.24e-15 72.8
Msa1030550 AT5G46590.1 30.469 128 85 3 18 142 6 132 3.66e-15 72.4
Msa1030550 AT5G46590.2 30.469 128 85 3 18 142 6 132 4.06e-15 72.0
Msa1030550 AT5G66300.1 32.558 129 81 5 18 142 12 138 4.89e-15 72.0
Msa1030550 AT4G17980.1 28.105 153 104 5 18 165 6 157 5.87e-15 71.6
Msa1030550 AT1G69490.1 32.031 128 80 5 18 142 9 132 7.09e-15 71.2
Msa1030550 AT3G04060.1 33.077 130 81 5 16 142 18 144 1.00e-14 71.6
Msa1030550 AT4G35580.1 28.643 199 114 7 18 192 9 203 1.44e-14 71.6
Msa1030550 AT5G24590.2 27.717 184 110 5 18 181 13 193 1.48e-14 71.6
Msa1030550 AT4G17980.2 28.105 153 104 5 18 165 6 157 1.56e-14 70.9
Msa1030550 AT4G35580.2 28.643 199 114 7 18 192 9 203 1.75e-14 71.6
Msa1030550 AT4G35580.3 28.643 199 114 7 18 192 9 203 1.86e-14 71.2
Msa1030550 AT4G27410.2 29.167 168 109 7 16 178 12 174 2.60e-14 70.1
Msa1030550 AT1G79580.3 30.534 131 86 4 16 142 15 144 2.80e-14 70.5
Msa1030550 AT1G79580.5 30.534 131 86 4 16 142 15 144 2.80e-14 70.5
Msa1030550 AT1G79580.4 30.534 131 86 4 16 142 15 144 2.80e-14 70.5
Msa1030550 AT1G79580.2 30.534 131 86 4 16 142 15 144 2.80e-14 70.5
Msa1030550 AT1G79580.1 30.534 131 86 4 16 142 15 144 2.80e-14 70.5
Msa1030550 AT3G12977.1 31.884 138 86 6 18 151 18 151 5.34e-14 68.9
Msa1030550 AT1G33060.1 32.624 141 90 5 5 142 11 149 7.81e-14 69.7
Msa1030550 AT1G33060.2 32.624 141 90 5 5 142 11 149 7.82e-14 69.7
Msa1030550 AT1G12260.2 28.387 155 105 5 18 168 7 159 8.23e-14 68.9
Msa1030550 AT5G62380.2 28.777 139 93 5 12 146 1 137 9.97e-14 68.9
Msa1030550 AT5G62380.1 28.777 139 93 5 12 146 1 137 9.97e-14 68.9
Msa1030550 AT1G12260.1 28.387 155 105 5 18 168 7 159 1.33e-13 68.6
Msa1030550 AT1G62700.1 27.329 161 111 5 17 173 6 164 1.59e-13 68.6
Msa1030550 AT1G62700.2 27.329 161 111 5 17 173 6 164 1.59e-13 68.6
Msa1030550 AT1G02230.1 30.714 140 86 4 16 148 1 136 2.02e-13 68.2
Msa1030550 AT4G36160.2 29.630 135 89 5 12 142 8 140 2.24e-13 68.2
Msa1030550 AT4G36160.1 29.630 135 89 5 12 142 4 136 2.26e-13 67.8
Msa1030550 AT4G36160.3 29.630 135 89 5 12 142 4 136 2.26e-13 67.8
Msa1030550 AT1G52890.1 31.538 130 82 5 16 142 12 137 2.50e-13 67.4
Msa1030550 AT1G34180.2 30.216 139 80 5 21 144 19 155 2.56e-13 68.2
Msa1030550 AT3G18400.1 32.812 128 79 6 18 142 5 128 3.52e-13 67.0
Msa1030550 AT1G34180.4 30.216 139 80 5 21 144 19 155 4.62e-13 67.4
Msa1030550 AT1G71930.2 31.008 129 83 5 18 142 9 135 6.31e-13 66.6
Msa1030550 AT1G71930.1 31.008 129 83 5 18 142 9 135 6.31e-13 66.6
Msa1030550 AT3G15500.1 30.769 130 83 4 16 142 12 137 6.39e-13 66.2
Msa1030550 AT5G09330.5 33.333 129 81 5 17 142 5 131 6.42e-13 67.0
Msa1030550 AT5G09330.2 33.333 129 81 5 17 142 5 131 6.42e-13 67.0
Msa1030550 AT5G09330.4 33.333 129 81 5 17 142 5 131 6.42e-13 67.0
Msa1030550 AT5G09330.3 33.333 129 81 5 17 142 5 131 6.42e-13 67.0
Msa1030550 AT5G09330.1 33.333 129 81 5 17 142 5 131 6.42e-13 67.0
Msa1030550 AT2G18060.3 28.571 133 89 5 14 142 5 135 1.64e-12 65.5
Msa1030550 AT2G18060.1 28.571 133 89 5 14 142 5 135 1.64e-12 65.5
Msa1030550 AT1G32510.1 30.216 139 83 5 15 142 3 138 3.81e-12 63.9
Msa1030550 AT4G27410.3 26.230 183 112 7 16 178 12 191 6.04e-12 63.5
Msa1030550 AT4G28530.1 30.405 148 80 6 15 142 7 151 8.25e-12 63.5
Msa1030550 AT4G01540.3 28.342 187 119 9 16 193 2 182 1.28e-11 63.2
Msa1030550 AT4G01540.4 28.342 187 119 9 16 193 2 182 1.40e-11 62.8
Msa1030550 AT4G01540.2 28.342 187 119 9 16 193 2 182 1.53e-11 62.8
Msa1030550 AT4G01540.1 28.342 187 119 9 16 193 2 182 1.69e-11 62.8
Msa1030550 AT3G49530.1 28.906 128 87 3 18 142 13 139 2.09e-11 62.4
Msa1030550 AT1G33280.1 31.298 131 84 5 18 144 8 136 2.25e-11 62.0
Msa1030550 AT3G49530.2 28.906 128 87 3 18 142 13 139 2.74e-11 62.0
Msa1030550 AT4G10350.1 31.298 131 84 5 18 144 9 137 3.82e-11 61.2
Msa1030550 AT3G44350.2 27.500 200 121 8 15 193 2 198 4.62e-11 60.5
Msa1030550 AT5G64530.2 30.534 131 76 6 16 144 1 118 6.74e-11 58.5
Msa1030550 AT1G32770.1 29.197 137 91 5 10 142 8 142 7.59e-11 60.5
Msa1030550 AT1G32870.3 28.070 171 110 6 1 165 30 193 8.28e-11 60.8

Find 57 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCATTGTCTTCAATTTAT+AGG 0.223937 7_1:-64694139 None:intergenic
GAAAGAACACATTTGTTTAC+TGG 0.255170 7_1:+64694763 Msa1030550:CDS
CTTCACTTGCTAAGAACTTT+TGG 0.256930 7_1:+64694191 Msa1030550:five_prime_UTR
TGAAACTACCTGTTGGATTC+AGG 0.274408 7_1:+64694268 Msa1030550:CDS
GGTACTATTTCTTTGATATC+AGG 0.295817 7_1:+64694634 Msa1030550:CDS
GAACCGTAGGTTCGAAAATA+TGG 0.299363 7_1:+64694656 Msa1030550:CDS
AAAGCATAGTGAGCTAGTTT+TGG 0.338409 7_1:+64694236 Msa1030550:CDS
AGAAATGAAACATGAGTTCA+TGG 0.371014 7_1:-64695235 None:intergenic
TCGAATTCTGACAGGACATT+TGG 0.373254 7_1:-64694357 None:intergenic
CAGGAATTGAGGTTTGTGCT+TGG 0.441832 7_1:+64695363 Msa1030550:three_prime_UTR
GAATTTAGTTGTAAGTCCTA+TGG 0.472907 7_1:+64695309 Msa1030550:three_prime_UTR
TGAGTTCATGGAGAGGGAGG+AGG 0.476558 7_1:-64695223 None:intergenic
AAATCAATCACTGTTGGTGT+GGG 0.492009 7_1:-64695172 None:intergenic
GGATCAAACCTGAATCCAAC+AGG 0.495450 7_1:-64694276 None:intergenic
ACAGGACATTTGGAAGAGGT+TGG 0.514459 7_1:-64694347 None:intergenic
GAGTTCATGGAGAGGGAGGA+GGG 0.516664 7_1:-64695222 None:intergenic
AATATGGATGCAAGAAGAGC+TGG 0.518416 7_1:+64694672 Msa1030550:CDS
GAGCCATGGAAGCTGCCAAA+AGG 0.533677 7_1:+64694393 Msa1030550:CDS
GAAATCAATCACTGTTGGTG+TGG 0.538024 7_1:-64695173 None:intergenic
GAAGAGCTGGAAATGGTGAA+TGG 0.539470 7_1:+64694685 Msa1030550:CDS
AGTTCATGGAGAGGGAGGAG+GGG 0.541559 7_1:-64695221 None:intergenic
TTTGTTTACTGGAGAATGCA+AGG 0.541628 7_1:+64694774 Msa1030550:CDS
CTATCGGATATTTAAGATGA+AGG 0.543622 7_1:+64695126 Msa1030550:CDS
GAGAGAGAAAGAGAGAGACT+AGG 0.545275 7_1:-64694090 None:intergenic
TCTACAAGCTCATGATCAGT+TGG 0.547810 7_1:-64694297 None:intergenic
GAAATACATCGAATTCTGAC+AGG 0.551455 7_1:-64694365 None:intergenic
GGACCTCTTCAAAGCATATG+AGG 0.554252 7_1:+64694613 Msa1030550:CDS
ATTGAGAATAATTGTTGTGT+GGG 0.561181 7_1:+64695471 Msa1030550:three_prime_UTR
TCTGACAGGACATTTGGAAG+AGG 0.561916 7_1:-64694351 None:intergenic
ATGTATTTCAAACTGAGCCA+TGG 0.561975 7_1:+64694379 Msa1030550:CDS
TGAAACATGAGTTCATGGAG+AGG 0.563520 7_1:-64695230 None:intergenic
ATAATGCACATAATTAGTAA+AGG 0.565113 7_1:-64695435 None:intergenic
GATGCAAGAAGAGCTGGAAA+TGG 0.566075 7_1:+64694678 Msa1030550:CDS
AATTGAGAATAATTGTTGTG+TGG 0.569112 7_1:+64695470 Msa1030550:three_prime_UTR
ATCAATCACTGTTGGTGTGG+GGG 0.572846 7_1:-64695170 None:intergenic
AAGCAACAATCAGCATCACT+TGG 0.576110 7_1:-64695509 None:intergenic
ATGTAGGTGTACTCAGCTGT+AGG 0.589112 7_1:+64695100 Msa1030550:intron
ACTCAGCTGTAGGTGCCTAT+CGG 0.590616 7_1:+64695110 Msa1030550:CDS
GTACCTCATATGCTTTGAAG+AGG 0.594155 7_1:-64694616 None:intergenic
TCTTTGATATCAGGAACCGT+AGG 0.597083 7_1:+64694643 Msa1030550:CDS
GATGGTATGAAACTACCTGT+TGG 0.597386 7_1:+64694261 Msa1030550:CDS
AGAGACACTTGCATCTTCCA+TGG 0.599268 7_1:-64695197 None:intergenic
CTTCATCTTAAATATCCGAT+AGG 0.611455 7_1:-64695125 None:intergenic
ACATGAGTTCATGGAGAGGG+AGG 0.614894 7_1:-64695226 None:intergenic
GTGAATGGAGAATATATGAG+AGG 0.620604 7_1:+64694700 Msa1030550:CDS
GAAACATGAGTTCATGGAGA+GGG 0.632009 7_1:-64695229 None:intergenic
AATCAATCACTGTTGGTGTG+GGG 0.641402 7_1:-64695171 None:intergenic
TTGTAGATTATTATCTGACG+AGG 0.656467 7_1:+64694313 Msa1030550:CDS
AGGATGCATGAATTTGTCAT+CGG 0.657194 7_1:+64694825 Msa1030550:CDS
GTCAAGCTCTGATGACACAA+TGG 0.657620 7_1:+64694802 Msa1030550:CDS
ATGGTGAAATCAATCACTGT+TGG 0.663372 7_1:-64695178 None:intergenic
TCGGATATTTAAGATGAAGG+TGG 0.674233 7_1:+64695129 Msa1030550:CDS
AACAGTGATTGATTTCACCA+TGG 0.680653 7_1:+64695180 Msa1030550:CDS
GAGAATATATGAGAGGAACG+AGG 0.682363 7_1:+64694707 Msa1030550:CDS
AAGCTCTGATGACACAATGG+AGG 0.685488 7_1:+64694805 Msa1030550:CDS
CAAGTGAAGAAAGAGTTGTG+TGG 0.710718 7_1:-64694177 None:intergenic
GTTTACTGGAGAATGCAAGG+AGG 0.781160 7_1:+64694777 Msa1030550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAACTAAAAAAAAATATGA+TGG - chr7_1:64695287-64695306 None:intergenic 10.0%
!! TAAACTAAAAAAAAATATGA+TGG - chr7_1:64695287-64695306 None:intergenic 10.0%
!! ATCATAAGAAAAAGAATGAA+GGG - chr7_1:64694434-64694453 None:intergenic 20.0%
!!! ACACAAGGAAAATTTTAAAA+GGG - chr7_1:64694495-64694514 None:intergenic 20.0%
!!! AACACAAGGAAAATTTTAAA+AGG - chr7_1:64694496-64694515 None:intergenic 20.0%
!! CCATCATAAGATTAATTAAA+GGG - chr7_1:64694527-64694546 None:intergenic 20.0%
!! TCCATCATAAGATTAATTAA+AGG - chr7_1:64694528-64694547 None:intergenic 20.0%
!!! TACAATTTTTCATCAAACTA+AGG + chr7_1:64694848-64694867 Msa1030550:CDS 20.0%
!! ATCACTATAAAAAAACTATG+AGG + chr7_1:64694940-64694959 Msa1030550:intron 20.0%
!!! TTTGAAGTAATAATGTGTTT+TGG + chr7_1:64694969-64694988 Msa1030550:intron 20.0%
!!! TTTTGAACTCAAATTAATGT+AGG + chr7_1:64695084-64695103 Msa1030550:intron 20.0%
!! ATAATGCACATAATTAGTAA+AGG - chr7_1:64695438-64695457 None:intergenic 20.0%
!! ATCATAAGAAAAAGAATGAA+GGG - chr7_1:64694434-64694453 None:intergenic 20.0%
!!! ACACAAGGAAAATTTTAAAA+GGG - chr7_1:64694495-64694514 None:intergenic 20.0%
!!! AACACAAGGAAAATTTTAAA+AGG - chr7_1:64694496-64694515 None:intergenic 20.0%
!! CCATCATAAGATTAATTAAA+GGG - chr7_1:64694527-64694546 None:intergenic 20.0%
!! TCCATCATAAGATTAATTAA+AGG - chr7_1:64694528-64694547 None:intergenic 20.0%
!!! TACAATTTTTCATCAAACTA+AGG + chr7_1:64694848-64694867 Msa1030550:CDS 20.0%
!! ATCACTATAAAAAAACTATG+AGG + chr7_1:64694940-64694959 Msa1030550:intron 20.0%
!!! TTTGAAGTAATAATGTGTTT+TGG + chr7_1:64694969-64694988 Msa1030550:intron 20.0%
!!! TTTTGAACTCAAATTAATGT+AGG + chr7_1:64695084-64695103 Msa1030550:intron 20.0%
!! ATAATGCACATAATTAGTAA+AGG - chr7_1:64695438-64695457 None:intergenic 20.0%
!! AAGCATTGTCTTCAATTTAT+AGG - chr7_1:64694142-64694161 None:intergenic 25.0%
!! CTATAATTTCACAAACCTTT+TGG - chr7_1:64694411-64694430 None:intergenic 25.0%
! GATCATAAGAAAAAGAATGA+AGG - chr7_1:64694435-64694454 None:intergenic 25.0%
! CCCTTTAATTAATCTTATGA+TGG + chr7_1:64694524-64694543 Msa1030550:intron 25.0%
!!! ATTTGTTTTCTGCAGATAAT+AGG + chr7_1:64694592-64694611 Msa1030550:intron 25.0%
!!! AAACATGTTTTTTTGTCCAT+AGG - chr7_1:64695328-64695347 None:intergenic 25.0%
!!! GTTTTATCTTTCTAGTTTTC+AGG + chr7_1:64695344-64695363 Msa1030550:three_prime_UTR 25.0%
! AATTGAGAATAATTGTTGTG+TGG + chr7_1:64695470-64695489 Msa1030550:three_prime_UTR 25.0%
! ATTGAGAATAATTGTTGTGT+GGG + chr7_1:64695471-64695490 Msa1030550:three_prime_UTR 25.0%
!! AAGCATTGTCTTCAATTTAT+AGG - chr7_1:64694142-64694161 None:intergenic 25.0%
!! CTATAATTTCACAAACCTTT+TGG - chr7_1:64694411-64694430 None:intergenic 25.0%
! GATCATAAGAAAAAGAATGA+AGG - chr7_1:64694435-64694454 None:intergenic 25.0%
! CCCTTTAATTAATCTTATGA+TGG + chr7_1:64694524-64694543 Msa1030550:intron 25.0%
!!! ATTTGTTTTCTGCAGATAAT+AGG + chr7_1:64694592-64694611 Msa1030550:intron 25.0%
!!! AAACATGTTTTTTTGTCCAT+AGG - chr7_1:64695328-64695347 None:intergenic 25.0%
!!! GTTTTATCTTTCTAGTTTTC+AGG + chr7_1:64695344-64695363 Msa1030550:three_prime_UTR 25.0%
! AATTGAGAATAATTGTTGTG+TGG + chr7_1:64695470-64695489 Msa1030550:three_prime_UTR 25.0%
! ATTGAGAATAATTGTTGTGT+GGG + chr7_1:64695471-64695490 Msa1030550:three_prime_UTR 25.0%
TTGTAGATTATTATCTGACG+AGG + chr7_1:64694313-64694332 Msa1030550:CDS 30.0%
! GGTACTATTTCTTTGATATC+AGG + chr7_1:64694634-64694653 Msa1030550:CDS 30.0%
GAAAGAACACATTTGTTTAC+TGG + chr7_1:64694763-64694782 Msa1030550:CDS 30.0%
!!! TGCTAGTTTTATGCAAAAAG+TGG + chr7_1:64695011-64695030 Msa1030550:intron 30.0%
CTTCATCTTAAATATCCGAT+AGG - chr7_1:64695128-64695147 None:intergenic 30.0%
! CTATCGGATATTTAAGATGA+AGG + chr7_1:64695126-64695145 Msa1030550:CDS 30.0%
AGAAATGAAACATGAGTTCA+TGG - chr7_1:64695238-64695257 None:intergenic 30.0%
GAATTTAGTTGTAAGTCCTA+TGG + chr7_1:64695309-64695328 Msa1030550:three_prime_UTR 30.0%
!!! TTTCTAGTTTTCAGGAATTG+AGG + chr7_1:64695352-64695371 Msa1030550:three_prime_UTR 30.0%
TTGTAGATTATTATCTGACG+AGG + chr7_1:64694313-64694332 Msa1030550:CDS 30.0%
! GGTACTATTTCTTTGATATC+AGG + chr7_1:64694634-64694653 Msa1030550:CDS 30.0%
GAAAGAACACATTTGTTTAC+TGG + chr7_1:64694763-64694782 Msa1030550:CDS 30.0%
!!! TGCTAGTTTTATGCAAAAAG+TGG + chr7_1:64695011-64695030 Msa1030550:intron 30.0%
CTTCATCTTAAATATCCGAT+AGG - chr7_1:64695128-64695147 None:intergenic 30.0%
! CTATCGGATATTTAAGATGA+AGG + chr7_1:64695126-64695145 Msa1030550:CDS 30.0%
AGAAATGAAACATGAGTTCA+TGG - chr7_1:64695238-64695257 None:intergenic 30.0%
GAATTTAGTTGTAAGTCCTA+TGG + chr7_1:64695309-64695328 Msa1030550:three_prime_UTR 30.0%
!!! TTTCTAGTTTTCAGGAATTG+AGG + chr7_1:64695352-64695371 Msa1030550:three_prime_UTR 30.0%
!! CTTCACTTGCTAAGAACTTT+TGG + chr7_1:64694191-64694210 Msa1030550:five_prime_UTR 35.0%
!! AAAGCATAGTGAGCTAGTTT+TGG + chr7_1:64694236-64694255 Msa1030550:CDS 35.0%
GAAATACATCGAATTCTGAC+AGG - chr7_1:64694368-64694387 None:intergenic 35.0%
ATGTATTTCAAACTGAGCCA+TGG + chr7_1:64694379-64694398 Msa1030550:CDS 35.0%
AAAGGGTTTCAAAGAACACA+AGG - chr7_1:64694510-64694529 None:intergenic 35.0%
! CATCCATATTTTCGAACCTA+CGG - chr7_1:64694662-64694681 None:intergenic 35.0%
GTGAATGGAGAATATATGAG+AGG + chr7_1:64694700-64694719 Msa1030550:CDS 35.0%
TTTGTTTACTGGAGAATGCA+AGG + chr7_1:64694774-64694793 Msa1030550:CDS 35.0%
AGGATGCATGAATTTGTCAT+CGG + chr7_1:64694825-64694844 Msa1030550:CDS 35.0%
! TCGGATATTTAAGATGAAGG+TGG + chr7_1:64695129-64695148 Msa1030550:CDS 35.0%
AAATCAATCACTGTTGGTGT+GGG - chr7_1:64695175-64695194 None:intergenic 35.0%
ATGGTGAAATCAATCACTGT+TGG - chr7_1:64695181-64695200 None:intergenic 35.0%
! AACAGTGATTGATTTCACCA+TGG + chr7_1:64695180-64695199 Msa1030550:CDS 35.0%
!! CTTCACTTGCTAAGAACTTT+TGG + chr7_1:64694191-64694210 Msa1030550:five_prime_UTR 35.0%
!! AAAGCATAGTGAGCTAGTTT+TGG + chr7_1:64694236-64694255 Msa1030550:CDS 35.0%
GAAATACATCGAATTCTGAC+AGG - chr7_1:64694368-64694387 None:intergenic 35.0%
ATGTATTTCAAACTGAGCCA+TGG + chr7_1:64694379-64694398 Msa1030550:CDS 35.0%
AAAGGGTTTCAAAGAACACA+AGG - chr7_1:64694510-64694529 None:intergenic 35.0%
! CATCCATATTTTCGAACCTA+CGG - chr7_1:64694662-64694681 None:intergenic 35.0%
GTGAATGGAGAATATATGAG+AGG + chr7_1:64694700-64694719 Msa1030550:CDS 35.0%
TTTGTTTACTGGAGAATGCA+AGG + chr7_1:64694774-64694793 Msa1030550:CDS 35.0%
AGGATGCATGAATTTGTCAT+CGG + chr7_1:64694825-64694844 Msa1030550:CDS 35.0%
! TCGGATATTTAAGATGAAGG+TGG + chr7_1:64695129-64695148 Msa1030550:CDS 35.0%
AAATCAATCACTGTTGGTGT+GGG - chr7_1:64695175-64695194 None:intergenic 35.0%
ATGGTGAAATCAATCACTGT+TGG - chr7_1:64695181-64695200 None:intergenic 35.0%
! AACAGTGATTGATTTCACCA+TGG + chr7_1:64695180-64695199 Msa1030550:CDS 35.0%
CAAGTGAAGAAAGAGTTGTG+TGG - chr7_1:64694180-64694199 None:intergenic 40.0%
!! AGAACTTTTGGTGACAACCA+TGG + chr7_1:64694203-64694222 Msa1030550:exon 40.0%
!!! AGTGAGCTAGTTTTGGAAGA+TGG + chr7_1:64694243-64694262 Msa1030550:CDS 40.0%
GATGGTATGAAACTACCTGT+TGG + chr7_1:64694261-64694280 Msa1030550:CDS 40.0%
TGAAACTACCTGTTGGATTC+AGG + chr7_1:64694268-64694287 Msa1030550:CDS 40.0%
TCTACAAGCTCATGATCAGT+TGG - chr7_1:64694300-64694319 None:intergenic 40.0%
TCGAATTCTGACAGGACATT+TGG - chr7_1:64694360-64694379 None:intergenic 40.0%
!! GTACCTCATATGCTTTGAAG+AGG - chr7_1:64694619-64694638 None:intergenic 40.0%
TCTTTGATATCAGGAACCGT+AGG + chr7_1:64694643-64694662 Msa1030550:CDS 40.0%
GAACCGTAGGTTCGAAAATA+TGG + chr7_1:64694656-64694675 Msa1030550:CDS 40.0%
AATATGGATGCAAGAAGAGC+TGG + chr7_1:64694672-64694691 Msa1030550:CDS 40.0%
GAGAATATATGAGAGGAACG+AGG + chr7_1:64694707-64694726 Msa1030550:CDS 40.0%
AATCAATCACTGTTGGTGTG+GGG - chr7_1:64695174-64695193 None:intergenic 40.0%
GAAATCAATCACTGTTGGTG+TGG - chr7_1:64695176-64695195 None:intergenic 40.0%
GAAACATGAGTTCATGGAGA+GGG - chr7_1:64695232-64695251 None:intergenic 40.0%
TGAAACATGAGTTCATGGAG+AGG - chr7_1:64695233-64695252 None:intergenic 40.0%
CAAGTGAAGAAAGAGTTGTG+TGG - chr7_1:64694180-64694199 None:intergenic 40.0%
!! AGAACTTTTGGTGACAACCA+TGG + chr7_1:64694203-64694222 Msa1030550:exon 40.0%
!!! AGTGAGCTAGTTTTGGAAGA+TGG + chr7_1:64694243-64694262 Msa1030550:CDS 40.0%
GATGGTATGAAACTACCTGT+TGG + chr7_1:64694261-64694280 Msa1030550:CDS 40.0%
TGAAACTACCTGTTGGATTC+AGG + chr7_1:64694268-64694287 Msa1030550:CDS 40.0%
TCTACAAGCTCATGATCAGT+TGG - chr7_1:64694300-64694319 None:intergenic 40.0%
TCGAATTCTGACAGGACATT+TGG - chr7_1:64694360-64694379 None:intergenic 40.0%
!! GTACCTCATATGCTTTGAAG+AGG - chr7_1:64694619-64694638 None:intergenic 40.0%
TCTTTGATATCAGGAACCGT+AGG + chr7_1:64694643-64694662 Msa1030550:CDS 40.0%
GAACCGTAGGTTCGAAAATA+TGG + chr7_1:64694656-64694675 Msa1030550:CDS 40.0%
AATATGGATGCAAGAAGAGC+TGG + chr7_1:64694672-64694691 Msa1030550:CDS 40.0%
GAGAATATATGAGAGGAACG+AGG + chr7_1:64694707-64694726 Msa1030550:CDS 40.0%
AATCAATCACTGTTGGTGTG+GGG - chr7_1:64695174-64695193 None:intergenic 40.0%
GAAATCAATCACTGTTGGTG+TGG - chr7_1:64695176-64695195 None:intergenic 40.0%
GAAACATGAGTTCATGGAGA+GGG - chr7_1:64695232-64695251 None:intergenic 40.0%
TGAAACATGAGTTCATGGAG+AGG - chr7_1:64695233-64695252 None:intergenic 40.0%
GAGAGAGAAAGAGAGAGACT+AGG - chr7_1:64694093-64694112 None:intergenic 45.0%
! ATGCTTTTCATCAGCAGCCA+TGG - chr7_1:64694223-64694242 None:intergenic 45.0%
GGATCAAACCTGAATCCAAC+AGG - chr7_1:64694279-64694298 None:intergenic 45.0%
ACAGGACATTTGGAAGAGGT+TGG - chr7_1:64694350-64694369 None:intergenic 45.0%
TCTGACAGGACATTTGGAAG+AGG - chr7_1:64694354-64694373 None:intergenic 45.0%
! AAACCTTTTGGCAGCTTCCA+TGG - chr7_1:64694399-64694418 None:intergenic 45.0%
GGACCTCTTCAAAGCATATG+AGG + chr7_1:64694613-64694632 Msa1030550:CDS 45.0%
GATGCAAGAAGAGCTGGAAA+TGG + chr7_1:64694678-64694697 Msa1030550:CDS 45.0%
GAAGAGCTGGAAATGGTGAA+TGG + chr7_1:64694685-64694704 Msa1030550:CDS 45.0%
GTTTACTGGAGAATGCAAGG+AGG + chr7_1:64694777-64694796 Msa1030550:CDS 45.0%
! GTCAAGCTCTGATGACACAA+TGG + chr7_1:64694802-64694821 Msa1030550:CDS 45.0%
! AAGCTCTGATGACACAATGG+AGG + chr7_1:64694805-64694824 Msa1030550:CDS 45.0%
ATGTAGGTGTACTCAGCTGT+AGG + chr7_1:64695100-64695119 Msa1030550:intron 45.0%
ATCAATCACTGTTGGTGTGG+GGG - chr7_1:64695173-64695192 None:intergenic 45.0%
AGAGACACTTGCATCTTCCA+TGG - chr7_1:64695200-64695219 None:intergenic 45.0%
CAGGAATTGAGGTTTGTGCT+TGG + chr7_1:64695363-64695382 Msa1030550:three_prime_UTR 45.0%
GAGAGAGAAAGAGAGAGACT+AGG - chr7_1:64694093-64694112 None:intergenic 45.0%
! ATGCTTTTCATCAGCAGCCA+TGG - chr7_1:64694223-64694242 None:intergenic 45.0%
GGATCAAACCTGAATCCAAC+AGG - chr7_1:64694279-64694298 None:intergenic 45.0%
ACAGGACATTTGGAAGAGGT+TGG - chr7_1:64694350-64694369 None:intergenic 45.0%
TCTGACAGGACATTTGGAAG+AGG - chr7_1:64694354-64694373 None:intergenic 45.0%
! AAACCTTTTGGCAGCTTCCA+TGG - chr7_1:64694399-64694418 None:intergenic 45.0%
GGACCTCTTCAAAGCATATG+AGG + chr7_1:64694613-64694632 Msa1030550:CDS 45.0%
GATGCAAGAAGAGCTGGAAA+TGG + chr7_1:64694678-64694697 Msa1030550:CDS 45.0%
GAAGAGCTGGAAATGGTGAA+TGG + chr7_1:64694685-64694704 Msa1030550:CDS 45.0%
GTTTACTGGAGAATGCAAGG+AGG + chr7_1:64694777-64694796 Msa1030550:CDS 45.0%
! GTCAAGCTCTGATGACACAA+TGG + chr7_1:64694802-64694821 Msa1030550:CDS 45.0%
! AAGCTCTGATGACACAATGG+AGG + chr7_1:64694805-64694824 Msa1030550:CDS 45.0%
ATGTAGGTGTACTCAGCTGT+AGG + chr7_1:64695100-64695119 Msa1030550:intron 45.0%
ATCAATCACTGTTGGTGTGG+GGG - chr7_1:64695173-64695192 None:intergenic 45.0%
AGAGACACTTGCATCTTCCA+TGG - chr7_1:64695200-64695219 None:intergenic 45.0%
CAGGAATTGAGGTTTGTGCT+TGG + chr7_1:64695363-64695382 Msa1030550:three_prime_UTR 45.0%
ACTCAGCTGTAGGTGCCTAT+CGG + chr7_1:64695110-64695129 Msa1030550:CDS 50.0%
ACATGAGTTCATGGAGAGGG+AGG - chr7_1:64695229-64695248 None:intergenic 50.0%
ACTCAGCTGTAGGTGCCTAT+CGG + chr7_1:64695110-64695129 Msa1030550:CDS 50.0%
ACATGAGTTCATGGAGAGGG+AGG - chr7_1:64695229-64695248 None:intergenic 50.0%
GAGCCATGGAAGCTGCCAAA+AGG + chr7_1:64694393-64694412 Msa1030550:CDS 55.0%
AGTTCATGGAGAGGGAGGAG+GGG - chr7_1:64695224-64695243 None:intergenic 55.0%
GAGTTCATGGAGAGGGAGGA+GGG - chr7_1:64695225-64695244 None:intergenic 55.0%
TGAGTTCATGGAGAGGGAGG+AGG - chr7_1:64695226-64695245 None:intergenic 55.0%
GAGCCATGGAAGCTGCCAAA+AGG + chr7_1:64694393-64694412 Msa1030550:CDS 55.0%
AGTTCATGGAGAGGGAGGAG+GGG - chr7_1:64695224-64695243 None:intergenic 55.0%
GAGTTCATGGAGAGGGAGGA+GGG - chr7_1:64695225-64695244 None:intergenic 55.0%
TGAGTTCATGGAGAGGGAGG+AGG - chr7_1:64695226-64695245 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 64694049 64695528 64694049 ID=Msa1030550;Name=Msa1030550
chr7_1 mRNA 64694049 64695528 64694049 ID=Msa1030550-mRNA-1;Parent=Msa1030550;Name=Msa1030550-mRNA-1;_AED=0.08;_eAED=0.08;_QI=173|1|1|1|1|1|3|288|196
chr7_1 exon 64694049 64694414 64694049 ID=Msa1030550-mRNA-1:exon:14241;Parent=Msa1030550-mRNA-1
chr7_1 exon 64694607 64694869 64694607 ID=Msa1030550-mRNA-1:exon:14242;Parent=Msa1030550-mRNA-1
chr7_1 exon 64695106 64695528 64695106 ID=Msa1030550-mRNA-1:exon:14243;Parent=Msa1030550-mRNA-1
chr7_1 five_prime_UTR 64694049 64694221 64694049 ID=Msa1030550-mRNA-1:five_prime_utr;Parent=Msa1030550-mRNA-1
chr7_1 CDS 64694222 64694414 64694222 ID=Msa1030550-mRNA-1:cds;Parent=Msa1030550-mRNA-1
chr7_1 CDS 64694607 64694869 64694607 ID=Msa1030550-mRNA-1:cds;Parent=Msa1030550-mRNA-1
chr7_1 CDS 64695106 64695240 64695106 ID=Msa1030550-mRNA-1:cds;Parent=Msa1030550-mRNA-1
chr7_1 three_prime_UTR 64695241 64695528 64695241 ID=Msa1030550-mRNA-1:three_prime_utr;Parent=Msa1030550-mRNA-1
Gene Sequence

>Msa1030550

ATGGCTGCTGATGAAAAGCATAGTGAGCTAGTTTTGGAAGATGGTATGAAACTACCTGTTGGATTCAGGTTTGATCCAACTGATCATGAGCTTGTAGATTATTATCTGACGAGGAAAGTCTGTAACCAACCTCTTCCAAATGTCCTGTCAGAATTCGATGTATTTCAAACTGAGCCATGGAAGCTGCCAAAAGATAATAGGACCTCTTCAAAGCATATGAGGTACTATTTCTTTGATATCAGGAACCGTAGGTTCGAAAATATGGATGCAAGAAGAGCTGGAAATGGTGAATGGAGAATATATGAGAGGAACGAGGAGTTTGCTCTTTCAAACAATCAATTGATTGCGAGAAAGAACACATTTGTTTACTGGAGAATGCAAGGAGGAAGTCAAGCTCTGATGACACAATGGAGGATGCATGAATTTGTCATCGGTACAATTTTTCATCAAACTAAGGTGTACTCAGCTGTAGGTGCCTATCGGATATTTAAGATGAAGGTGGCAAAAGCTGAGAAGAAAGCCCCCACACCAACAGTGATTGATTTCACCATGGAAGATGCAAGTGTCTCTGCCCCTCCTCCCTCTCCATGA
Protein sequence

>Msa1030550

MAADEKHSELVLEDGMKLPVGFRFDPTDHELVDYYLTRKVCNQPLPNVLSEFDVFQTEPWKLPKDNRTSSKHMRYYFFDIRNRRFENMDARRAGNGEWRIYERNEEFALSNNQLIARKNTFVYWRMQGGSQALMTQWRMHEFVIGTIFHQTKVYSAVGAYRIFKMKVAKAEKKAPTPTVIDFTMEDASVSAPPPSP*