AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1031280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1031280 MtrunA17_Chr7g0252721 93.133 233 10 2 1 227 1 233 5.71e-161 444
Msa1031280 MtrunA17_Chr1g0213561 41.872 203 102 3 18 217 14 203 2.63e-44 147
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1031280 AT3G53860.1 45.872 218 109 2 8 218 52 267 2.45e-62 196
Msa1031280 AT2G31600.3 44.444 234 105 5 8 218 56 287 2.81e-62 197
Msa1031280 AT2G31600.1 44.444 234 105 5 8 218 56 287 2.81e-62 197
Msa1031280 AT2G31600.4 44.444 234 105 5 8 218 56 287 2.81e-62 197
Msa1031280 AT2G31600.5 44.444 234 105 5 8 218 56 287 2.81e-62 197
Msa1031280 AT1G05860.1 45.570 237 108 4 1 218 32 266 1.39e-59 189
Msa1031280 AT1G05860.2 45.570 237 108 4 1 218 32 266 1.39e-59 189
Msa1031280 AT1G05860.3 46.951 164 66 4 1 145 32 193 3.34e-37 130
Msa1031280 AT2G31600.2 43.750 160 65 5 8 144 56 213 2.55e-35 125
Msa1031280 AT2G31600.6 43.750 160 65 5 8 144 56 213 2.55e-35 125

Find 87 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTGCTTCGTGCTGTAAAA+AGG 0.184649 7_1:+65805364 Msa1031280:CDS
AAGATAATGATGCTGATTAT+TGG 0.208253 7_1:+65805525 Msa1031280:three_prime_UTR
ATACAGAGTCGCAAAATTTA+AGG 0.228058 7_1:-65805708 None:intergenic
TAACTCTGTTTCTCGGATAT+TGG 0.233652 7_1:-65805600 None:intergenic
TGAATTTGGCGGACAAATTC+AGG 0.235095 7_1:-65805444 None:intergenic
CATAATAACAATTTGTTGAT+TGG 0.244399 7_1:+65805799 Msa1031280:three_prime_UTR
GCTGTTACCGAGGTCATTAC+TGG 0.271359 7_1:+65803347 Msa1031280:CDS
ATTGGTGGTGGTGGATTTAT+TGG 0.277786 7_1:-65803178 None:intergenic
AACAATTTGTTGATTGGATC+TGG 0.288901 7_1:+65805805 Msa1031280:three_prime_UTR
ATAATCAGCATCATTATCTT+TGG 0.295406 7_1:-65805522 None:intergenic
AGGTCATTACTGGGCGTTAA+TGG 0.298106 7_1:+65803357 Msa1031280:CDS
GGTTAATCAGTCTCAGGTTT+AGG 0.308564 7_1:-65805501 None:intergenic
GGACTAGGCTTACCATTAAC+TGG 0.312740 7_1:-65805402 None:intergenic
AAAGACATTGCACAGTATAT+AGG 0.325419 7_1:+65805566 Msa1031280:three_prime_UTR
GCTTTATGCAGTTTGCCAAC+AGG 0.332413 7_1:+65805261 Msa1031280:intron
GCTGCTGCTGCTGTTGATAA+TGG 0.339422 7_1:+65803478 Msa1031280:CDS
GGTCATAACGTCCCAGTTAA+TGG 0.342126 7_1:+65805390 Msa1031280:CDS
GGAAGATGAAGAAAGAAATT+CGG 0.342169 7_1:+65803438 Msa1031280:CDS
CTAAGTACAGAGAGTATTAC+TGG 0.348566 7_1:+65803392 Msa1031280:CDS
GTTGCACCGCCGTGGCAAAA+AGG 0.349754 7_1:+65803611 Msa1031280:CDS
CGAGAAACAGAGTTATGATT+TGG 0.355665 7_1:+65805608 Msa1031280:three_prime_UTR
CTAGCATACTCACAATTTAC+AGG 0.364573 7_1:-65805679 None:intergenic
AGAGAGTATTACTGGACTTA+TGG 0.365244 7_1:+65803400 Msa1031280:CDS
ATTGAGATCCATGGTTCCTT+TGG 0.385033 7_1:+65805308 Msa1031280:CDS
GAAGATGAAGAAAGAAATTC+GGG 0.400485 7_1:+65803439 Msa1031280:CDS
AAGACATTGCACAGTATATA+GGG 0.406322 7_1:+65805567 Msa1031280:three_prime_UTR
ATCTTTGGTTAATCAGTCTC+AGG 0.414837 7_1:-65805507 None:intergenic
CCACGATAAAGCTTCTGATT+AGG 0.415729 7_1:-65803589 None:intergenic
GCCAATAAAATGACCGGGTA+AGG 0.421804 7_1:-65805731 None:intergenic
CACGGCGGAGGCGGCGGACG+AGG 0.423786 7_1:-65803309 None:intergenic
GTCTGACACGGCGGAGGCGG+CGG 0.433953 7_1:-65803315 None:intergenic
GAGTCTTCCATTGGTGGTGG+TGG 0.438608 7_1:-65803187 None:intergenic
AATAGATGTAACTGCGGACT+AGG 0.448722 7_1:-65805417 None:intergenic
GACCTCGCGGCGGGTTAAAA+CGG 0.448768 7_1:-65803287 None:intergenic
TCGCCTAATTGGTAATGAGC+AGG 0.451526 7_1:-65805336 None:intergenic
GGAACCATGGATCTCAATAC+TGG 0.454340 7_1:-65805303 None:intergenic
TGCCCTGCTCATTACCAATT+AGG 0.459872 7_1:+65805333 Msa1031280:CDS
TGCTGTTGAGAGTCTTCCAT+TGG 0.466609 7_1:-65803196 None:intergenic
CTTTCTTCATCTTCCTTGAA+CGG 0.468778 7_1:-65803430 None:intergenic
GTTGTCTGACACGGCGGAGG+CGG 0.468951 7_1:-65803318 None:intergenic
CAAATCATAACTCTGTTTCT+CGG 0.478717 7_1:-65805607 None:intergenic
TAGAATGCCAATAAAATGAC+CGG 0.482423 7_1:-65805737 None:intergenic
GGCGTTAATGGAAGAGCTTA+AGG 0.485899 7_1:+65803369 Msa1031280:CDS
TAAACGAAAAGCTGCGCGCA+AGG 0.489819 7_1:+65805470 Msa1031280:CDS
ATAATGGTGATGACTTTGTT+CGG 0.491789 7_1:+65803494 Msa1031280:CDS
TGTTGAGAGTCTTCCATTGG+TGG 0.497279 7_1:-65803193 None:intergenic
GCGGACGAGGACCTCGCGGC+GGG 0.498422 7_1:-65803296 None:intergenic
CGCCTAATTGGTAATGAGCA+GGG 0.500265 7_1:-65805335 None:intergenic
CTTTATGCAGTTTGCCAACA+GGG 0.500980 7_1:+65805262 Msa1031280:intron
TTATGGAAGGAGTCCGTTCA+AGG 0.519406 7_1:+65803417 Msa1031280:CDS
TGCGACTCTGTATCCTTACC+CGG 0.530731 7_1:+65805718 Msa1031280:three_prime_UTR
TCGGCACCGTCGATTGTCAT+CGG 0.530875 7_1:-65803238 None:intergenic
AATAAATCCACCACCACCAA+TGG 0.548677 7_1:+65803180 Msa1031280:exon
GCACAGTATATAGGGAGGGC+AGG 0.552643 7_1:+65805575 Msa1031280:three_prime_UTR
GTCATCGGCGCGATTTGTTG+CGG 0.555912 7_1:-65803223 None:intergenic
ACTGGCTTATTACAAAAGAC+TGG 0.557434 7_1:-65805285 None:intergenic
GACTAGGCTTACCATTAACT+GGG 0.570203 7_1:-65805401 None:intergenic
TGAGAGTCTTCCATTGGTGG+TGG 0.572325 7_1:-65803190 None:intergenic
TTACAAAAGACTGGCCCTGT+TGG 0.574396 7_1:-65805276 None:intergenic
CTGTTACCGAGGTCATTACT+GGG 0.578965 7_1:+65803348 Msa1031280:CDS
CTTAGTAAGAGCCATAGCCT+TGG 0.581621 7_1:-65803539 None:intergenic
TTTGTTCGGTGTGCTTCTAG+TGG 0.584923 7_1:+65803508 Msa1031280:CDS
AGAGTTGTCTGACACGGCGG+AGG 0.587249 7_1:-65803321 None:intergenic
TGGTTGCAAGACCAAGGCTA+TGG 0.587740 7_1:+65803528 Msa1031280:CDS
CAACTCTCTCGCTGTTACCG+AGG 0.590857 7_1:+65803337 Msa1031280:CDS
AGTATTACTGGACTTATGGA+AGG 0.608751 7_1:+65803404 Msa1031280:CDS
GGCGGACGAGGACCTCGCGG+CGG 0.609662 7_1:-65803297 None:intergenic
TAAGCCAGTATTGAGATCCA+TGG 0.612741 7_1:+65805299 Msa1031280:CDS
AGCGAGGGCTAAGTCTTCGT+CGG 0.616825 7_1:-65803257 None:intergenic
TTATCGTGGTTGCACCGCCG+TGG 0.625527 7_1:+65803603 Msa1031280:CDS
TTCTAGTGGTTGCAAGACCA+AGG 0.627196 7_1:+65803522 Msa1031280:CDS
ATCGGCGCGATTTGTTGCGG+TGG 0.628254 7_1:-65803220 None:intergenic
GGCGGCGGACGAGGACCTCG+CGG 0.628435 7_1:-65803300 None:intergenic
TAATGAGCAGGGCATACCAA+AGG 0.651701 7_1:-65805324 None:intergenic
AGAATGCCAATAAAATGACC+GGG 0.656066 7_1:-65805736 None:intergenic
CATTGCACAGTATATAGGGA+GGG 0.657863 7_1:+65805571 Msa1031280:three_prime_UTR
CTCGCGGCGGGTTAAAACGG+AGG 0.662601 7_1:-65803284 None:intergenic
ATCGCGCCGATGACAATCGA+CGG 0.665199 7_1:+65803232 Msa1031280:CDS
CCTAATCAGAAGCTTTATCG+TGG 0.666724 7_1:+65803589 Msa1031280:CDS
TTAAAACGGAGGAGTTAGCG+AGG 0.671046 7_1:-65803273 None:intergenic
AGGGCATACCAAAGGAACCA+TGG 0.672546 7_1:-65805316 None:intergenic
AGCGAGAGAGTTGTCTGACA+CGG 0.684863 7_1:-65803327 None:intergenic
ACATTGCACAGTATATAGGG+AGG 0.686301 7_1:+65805570 Msa1031280:three_prime_UTR
TAAAACGGAGGAGTTAGCGA+GGG 0.688548 7_1:-65803272 None:intergenic
GAGAGAGTTGTCTGACACGG+CGG 0.692611 7_1:-65803324 None:intergenic
GGAATTAATAGATGTAACTG+CGG 0.701946 7_1:-65805423 None:intergenic
TTAACGCCCAGTAATGACCT+CGG 0.702952 7_1:-65803354 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTGATTAATAACAAATA+TGG + chr7_1:65803738-65803757 Msa1031280:intron 10.0%
!!! TCAATTTTTCAAATTATTAT+CGG - chr7_1:65804171-65804190 None:intergenic 10.0%
!!! AATACTATTATTTTTTTGAC+TGG + chr7_1:65803862-65803881 Msa1031280:intron 15.0%
!!! ATACTATTATTTTTTTGACT+GGG + chr7_1:65803863-65803882 Msa1031280:intron 15.0%
!!! TTTGTTTGTTTATATGTTTT+TGG + chr7_1:65803940-65803959 Msa1031280:intron 15.0%
!! TTAAATTTAATTAGTAGGAT+AGG - chr7_1:65805084-65805103 None:intergenic 15.0%
!! AAGCATTAAATTTAATTAGT+AGG - chr7_1:65805089-65805108 None:intergenic 15.0%
!!! ATTTGGTAATTTGTGTTTTT+AGG + chr7_1:65803779-65803798 Msa1031280:intron 20.0%
!!! TTTGGTAATTTGTGTTTTTA+GGG + chr7_1:65803780-65803799 Msa1031280:intron 20.0%
!!! GAAGAAGATAATGTTTTTTT+TGG + chr7_1:65803817-65803836 Msa1031280:intron 20.0%
!!! AAATGTGATCATATATGTTT+TGG + chr7_1:65804057-65804076 Msa1031280:intron 20.0%
!!! TTCAAAATTGTTTTTCCTTT+CGG + chr7_1:65804340-65804359 Msa1031280:intron 20.0%
!!! CTTTTCGTTTATTTTGAATT+TGG - chr7_1:65805461-65805480 None:intergenic 20.0%
!!! CATAATAACAATTTGTTGAT+TGG + chr7_1:65805799-65805818 Msa1031280:three_prime_UTR 20.0%
!!! TACTGTATTGTTTTTGATGA+TGG + chr7_1:65803694-65803713 Msa1031280:intron 25.0%
! TAATTGTGTTTCTTGCAATT+TGG + chr7_1:65803762-65803781 Msa1031280:intron 25.0%
!!! TTTTTTTGGATTTTCTTGTG+TGG + chr7_1:65803831-65803850 Msa1031280:intron 25.0%
!!! TTATTTTTTTGACTGGGTTT+TGG + chr7_1:65803869-65803888 Msa1031280:intron 25.0%
!!! CTACTTTTTTCTCTTGTTTT+TGG + chr7_1:65804374-65804393 Msa1031280:intron 25.0%
! ACAAACACATATAGATTGAA+TGG + chr7_1:65804428-65804447 Msa1031280:intron 25.0%
! AAAAACTGTATAATGCAAGA+TGG - chr7_1:65805014-65805033 None:intergenic 25.0%
!! ATCTTGCATTATACAGTTTT+TGG + chr7_1:65805013-65805032 Msa1031280:intron 25.0%
!!! ACAGTTTTTGGTTTTTATAC+AGG + chr7_1:65805025-65805044 Msa1031280:intron 25.0%
! GTAATACATGAAAACAGAAA+AGG - chr7_1:65805175-65805194 None:intergenic 25.0%
!!! TTCGTTTATTTTGAATTTGG+CGG - chr7_1:65805458-65805477 None:intergenic 25.0%
! ATAATCAGCATCATTATCTT+TGG - chr7_1:65805525-65805544 None:intergenic 25.0%
!! AAGATAATGATGCTGATTAT+TGG + chr7_1:65805525-65805544 Msa1031280:three_prime_UTR 25.0%
! GGATTTATTGGAGAGATTTT+CGG - chr7_1:65803169-65803188 None:intergenic 30.0%
GGAAGATGAAGAAAGAAATT+CGG + chr7_1:65803438-65803457 Msa1031280:CDS 30.0%
GAAGATGAAGAAAGAAATTC+GGG + chr7_1:65803439-65803458 Msa1031280:CDS 30.0%
! ATAATGGTGATGACTTTGTT+CGG + chr7_1:65803494-65803513 Msa1031280:CDS 30.0%
!! TTGTGGTTGATGAGAAAAAT+GGG + chr7_1:65803913-65803932 Msa1031280:intron 30.0%
! TGTTGTTGTTCTTGACATAT+TGG + chr7_1:65804096-65804115 Msa1031280:intron 30.0%
ATTGACATAATTCAACGTGA+TGG + chr7_1:65804186-65804205 Msa1031280:intron 30.0%
ACATAAACACTTGTAGTCTA+TGG + chr7_1:65804402-65804421 Msa1031280:intron 30.0%
! ACATTGTCAATCTGAAATGT+TGG + chr7_1:65804503-65804522 Msa1031280:intron 30.0%
AATTGTAATTCAACAGTGTG+TGG + chr7_1:65804547-65804566 Msa1031280:intron 30.0%
ATTGTAATTCAACAGTGTGT+GGG + chr7_1:65804548-65804567 Msa1031280:intron 30.0%
CATGAACTGAATAAAGATTG+AGG - chr7_1:65804612-65804631 None:intergenic 30.0%
CTCAATCTTTATTCAGTTCA+TGG + chr7_1:65804610-65804629 Msa1031280:intron 30.0%
AAAAGGTCCAGGTTATTATT+CGG - chr7_1:65804638-65804657 None:intergenic 30.0%
! AATAATAACCTGGACCTTTT+TGG + chr7_1:65804638-65804657 Msa1031280:intron 30.0%
CTTGCTTATGATAGAAACTT+GGG + chr7_1:65804721-65804740 Msa1031280:intron 30.0%
GATAGAAACTTGGGTATTAT+AGG + chr7_1:65804730-65804749 Msa1031280:intron 30.0%
CCTTAATACTTACATCTCAA+GGG - chr7_1:65804821-65804840 None:intergenic 30.0%
CCTTGAGATGTAAGTATTAA+GGG + chr7_1:65804819-65804838 Msa1031280:intron 30.0%
! AACAGATATGTATTGATGCT+TGG - chr7_1:65804915-65804934 None:intergenic 30.0%
ATGCAGTGTACATGTTAAAA+GGG + chr7_1:65804957-65804976 Msa1031280:intron 30.0%
ATTACCTACAGAAGACAATT+CGG + chr7_1:65805224-65805243 Msa1031280:intron 30.0%
GGAATTAATAGATGTAACTG+CGG - chr7_1:65805426-65805445 None:intergenic 30.0%
AAAGACATTGCACAGTATAT+AGG + chr7_1:65805566-65805585 Msa1031280:three_prime_UTR 30.0%
AAGACATTGCACAGTATATA+GGG + chr7_1:65805567-65805586 Msa1031280:three_prime_UTR 30.0%
! CAAATCATAACTCTGTTTCT+CGG - chr7_1:65805610-65805629 None:intergenic 30.0%
ATACAGAGTCGCAAAATTTA+AGG - chr7_1:65805711-65805730 None:intergenic 30.0%
TAGAATGCCAATAAAATGAC+CGG - chr7_1:65805740-65805759 None:intergenic 30.0%
!! AACAATTTGTTGATTGGATC+TGG + chr7_1:65805805-65805824 Msa1031280:three_prime_UTR 30.0%
! GATTTTCGGCGTTTCTTTTT+GGG - chr7_1:65803155-65803174 None:intergenic 35.0%
! AGATTTTCGGCGTTTCTTTT+TGG - chr7_1:65803156-65803175 None:intergenic 35.0%
!! CTAAGTACAGAGAGTATTAC+TGG + chr7_1:65803392-65803411 Msa1031280:CDS 35.0%
!! AGAGAGTATTACTGGACTTA+TGG + chr7_1:65803400-65803419 Msa1031280:CDS 35.0%
AGTATTACTGGACTTATGGA+AGG + chr7_1:65803404-65803423 Msa1031280:CDS 35.0%
CTTTCTTCATCTTCCTTGAA+CGG - chr7_1:65803433-65803452 None:intergenic 35.0%
! AAAAACTCACCTTTTTGCCA+CGG - chr7_1:65803623-65803642 None:intergenic 35.0%
!! GGAATAATGAGTATTGACTG+TGG + chr7_1:65803890-65803909 Msa1031280:intron 35.0%
!! GTTGTGGTTGATGAGAAAAA+TGG + chr7_1:65803912-65803931 Msa1031280:intron 35.0%
CAACTTTCTCAGACTATTAC+AGG - chr7_1:65804030-65804049 None:intergenic 35.0%
ACAACAACACTTCTGATTGA+AGG - chr7_1:65804085-65804104 None:intergenic 35.0%
!!! GTAGTCATGTTTTAACCGAA+AGG - chr7_1:65804358-65804377 None:intergenic 35.0%
CATATAGATTGAATGGAACC+AGG + chr7_1:65804435-65804454 Msa1031280:intron 35.0%
AAAGGTCCAGGTTATTATTC+GGG - chr7_1:65804637-65804656 None:intergenic 35.0%
CAAGAACTTCGAATCAAACT+TGG - chr7_1:65804664-65804683 None:intergenic 35.0%
AGGATGACAAGATTGAAAGA+AGG - chr7_1:65804703-65804722 None:intergenic 35.0%
CCAAGTTTCTATCATAAGCA+AGG - chr7_1:65804723-65804742 None:intergenic 35.0%
CCTTGCTTATGATAGAAACT+TGG + chr7_1:65804720-65804739 Msa1031280:intron 35.0%
CCCTTGAGATGTAAGTATTA+AGG + chr7_1:65804818-65804837 Msa1031280:intron 35.0%
CCCTTAATACTTACATCTCA+AGG - chr7_1:65804822-65804841 None:intergenic 35.0%
! ATGTATTGATGCTTGGAAGT+TGG - chr7_1:65804908-65804927 None:intergenic 35.0%
CATGCAGTGTACATGTTAAA+AGG + chr7_1:65804956-65804975 Msa1031280:intron 35.0%
! CAATTCGGCTGATAAACTTT+TGG + chr7_1:65805239-65805258 Msa1031280:intron 35.0%
! ACTGGCTTATTACAAAAGAC+TGG - chr7_1:65805288-65805307 None:intergenic 35.0%
ATCTTTGGTTAATCAGTCTC+AGG - chr7_1:65805510-65805529 None:intergenic 35.0%
! TAACTCTGTTTCTCGGATAT+TGG - chr7_1:65805603-65805622 None:intergenic 35.0%
CGAGAAACAGAGTTATGATT+TGG + chr7_1:65805608-65805627 Msa1031280:three_prime_UTR 35.0%
CTAGCATACTCACAATTTAC+AGG - chr7_1:65805682-65805701 None:intergenic 35.0%
AGAATGCCAATAAAATGACC+GGG - chr7_1:65805739-65805758 None:intergenic 35.0%
!! ATTGGTGGTGGTGGATTTAT+TGG - chr7_1:65803181-65803200 None:intergenic 40.0%
AATAAATCCACCACCACCAA+TGG + chr7_1:65803180-65803199 Msa1031280:exon 40.0%
CCACGATAAAGCTTCTGATT+AGG - chr7_1:65803592-65803611 None:intergenic 40.0%
! CCTAATCAGAAGCTTTATCG+TGG + chr7_1:65803589-65803608 Msa1031280:CDS 40.0%
! TGGATTTTCTTGTGTGGCTT+TGG + chr7_1:65803837-65803856 Msa1031280:intron 40.0%
!! ATGAGTATTGACTGTGGTTG+TGG + chr7_1:65803896-65803915 Msa1031280:intron 40.0%
AATTCAACGTGATGGCATGT+CGG + chr7_1:65804194-65804213 Msa1031280:intron 40.0%
CTAACATGTATCAGTGTACC+TGG - chr7_1:65804456-65804475 None:intergenic 40.0%
TGTGTCTAGTGTCTGAATCA+TGG - chr7_1:65804486-65804505 None:intergenic 40.0%
CGAATCAAACTTGGCCAAAA+AGG - chr7_1:65804655-65804674 None:intergenic 40.0%
! GGAGAATTTTCCAAGTGCTT+GGG + chr7_1:65804840-65804859 Msa1031280:intron 40.0%
! GAGAATTTTCCAAGTGCTTG+GGG + chr7_1:65804841-65804860 Msa1031280:intron 40.0%
TTGCCTTCAGTTTAGTTGTG+TGG + chr7_1:65804933-65804952 Msa1031280:intron 40.0%
ATGCCACACAACTAAACTGA+AGG - chr7_1:65804939-65804958 None:intergenic 40.0%
CTTTATGCAGTTTGCCAACA+GGG + chr7_1:65805262-65805281 Msa1031280:intron 40.0%
! TAAGCCAGTATTGAGATCCA+TGG + chr7_1:65805299-65805318 Msa1031280:CDS 40.0%
ATTGAGATCCATGGTTCCTT+TGG + chr7_1:65805308-65805327 Msa1031280:CDS 40.0%
! GCAAGCATTTTTCGCCTAAT+TGG - chr7_1:65805350-65805369 None:intergenic 40.0%
CTTGCTTCGTGCTGTAAAAA+GGG + chr7_1:65805365-65805384 Msa1031280:CDS 40.0%
GACTAGGCTTACCATTAACT+GGG - chr7_1:65805404-65805423 None:intergenic 40.0%
AATAGATGTAACTGCGGACT+AGG - chr7_1:65805420-65805439 None:intergenic 40.0%
! TGAATTTGGCGGACAAATTC+AGG - chr7_1:65805447-65805466 None:intergenic 40.0%
GGTTAATCAGTCTCAGGTTT+AGG - chr7_1:65805504-65805523 None:intergenic 40.0%
ACATTGCACAGTATATAGGG+AGG + chr7_1:65805570-65805589 Msa1031280:three_prime_UTR 40.0%
CATTGCACAGTATATAGGGA+GGG + chr7_1:65805571-65805590 Msa1031280:three_prime_UTR 40.0%
!! TCCTTACCCGGTCATTTTAT+TGG + chr7_1:65805730-65805749 Msa1031280:three_prime_UTR 40.0%
TGTTGAGAGTCTTCCATTGG+TGG - chr7_1:65803196-65803215 None:intergenic 45.0%
TGCTGTTGAGAGTCTTCCAT+TGG - chr7_1:65803199-65803218 None:intergenic 45.0%
TAAAACGGAGGAGTTAGCGA+GGG - chr7_1:65803275-65803294 None:intergenic 45.0%
TTAAAACGGAGGAGTTAGCG+AGG - chr7_1:65803276-65803295 None:intergenic 45.0%
CTGTTACCGAGGTCATTACT+GGG + chr7_1:65803348-65803367 Msa1031280:CDS 45.0%
TTAACGCCCAGTAATGACCT+CGG - chr7_1:65803357-65803376 None:intergenic 45.0%
AGGTCATTACTGGGCGTTAA+TGG + chr7_1:65803357-65803376 Msa1031280:CDS 45.0%
GGCGTTAATGGAAGAGCTTA+AGG + chr7_1:65803369-65803388 Msa1031280:CDS 45.0%
TTATGGAAGGAGTCCGTTCA+AGG + chr7_1:65803417-65803436 Msa1031280:CDS 45.0%
!! TTTGTTCGGTGTGCTTCTAG+TGG + chr7_1:65803508-65803527 Msa1031280:CDS 45.0%
TTCTAGTGGTTGCAAGACCA+AGG + chr7_1:65803522-65803541 Msa1031280:CDS 45.0%
CTTAGTAAGAGCCATAGCCT+TGG - chr7_1:65803542-65803561 None:intergenic 45.0%
! TCAGCGTGTCATTGTCATTC+CGG - chr7_1:65804005-65804024 None:intergenic 45.0%
TCTAGTGTCTGAATCATGGC+TGG - chr7_1:65804482-65804501 None:intergenic 45.0%
CATGGACCCGAATAATAACC+TGG + chr7_1:65804628-65804647 Msa1031280:intron 45.0%
AAACTTGGCCAAAAAGGTCC+AGG - chr7_1:65804649-65804668 None:intergenic 45.0%
CATCTCATCCTACTTGACGT+GGG - chr7_1:65804766-65804785 None:intergenic 45.0%
AAGTAGGATGAGATGCTCGT+TGG + chr7_1:65804771-65804790 Msa1031280:intron 45.0%
! GGGAGAATTTTCCAAGTGCT+TGG + chr7_1:65804839-65804858 Msa1031280:intron 45.0%
TGCTTGGAAGTTGGAAGTCA+TGG - chr7_1:65804899-65804918 None:intergenic 45.0%
ATGGCTCATTCACATTGGTG+AGG - chr7_1:65804995-65805014 None:intergenic 45.0%
GCAAGATGGCTCATTCACAT+TGG - chr7_1:65805000-65805019 None:intergenic 45.0%
AGTAGGATAGGTACAGACAG+AGG - chr7_1:65805072-65805091 None:intergenic 45.0%
TCAGCCGAATTGTCTTCTGT+AGG - chr7_1:65805231-65805250 None:intergenic 45.0%
GCTTTATGCAGTTTGCCAAC+AGG + chr7_1:65805261-65805280 Msa1031280:intron 45.0%
! TTACAAAAGACTGGCCCTGT+TGG - chr7_1:65805279-65805298 None:intergenic 45.0%
GGAACCATGGATCTCAATAC+TGG - chr7_1:65805306-65805325 None:intergenic 45.0%
TAATGAGCAGGGCATACCAA+AGG - chr7_1:65805327-65805346 None:intergenic 45.0%
TGCCCTGCTCATTACCAATT+AGG + chr7_1:65805333-65805352 Msa1031280:CDS 45.0%
!! CGCCTAATTGGTAATGAGCA+GGG - chr7_1:65805338-65805357 None:intergenic 45.0%
!! TCGCCTAATTGGTAATGAGC+AGG - chr7_1:65805339-65805358 None:intergenic 45.0%
GCTTGCTTCGTGCTGTAAAA+AGG + chr7_1:65805364-65805383 Msa1031280:CDS 45.0%
GGTCATAACGTCCCAGTTAA+TGG + chr7_1:65805390-65805409 Msa1031280:CDS 45.0%
GGACTAGGCTTACCATTAAC+TGG - chr7_1:65805405-65805424 None:intergenic 45.0%
GCCAATAAAATGACCGGGTA+AGG - chr7_1:65805734-65805753 None:intergenic 45.0%
!!! ATTTAATTTATTGATTTTTT+TGG + chr7_1:65803668-65803687 Msa1031280:intron 5.0%
TGAGAGTCTTCCATTGGTGG+TGG - chr7_1:65803193-65803212 None:intergenic 50.0%
AGCGAGAGAGTTGTCTGACA+CGG - chr7_1:65803330-65803349 None:intergenic 50.0%
GCTGTTACCGAGGTCATTAC+TGG + chr7_1:65803347-65803366 Msa1031280:CDS 50.0%
! GCTGCTGCTGCTGTTGATAA+TGG + chr7_1:65803478-65803497 Msa1031280:CDS 50.0%
TGGTTGCAAGACCAAGGCTA+TGG + chr7_1:65803528-65803547 Msa1031280:CDS 50.0%
! AACTCACCTTTTTGCCACGG+CGG - chr7_1:65803620-65803639 None:intergenic 50.0%
!! GAGAAGCACCGACAAAGACA+CGG + chr7_1:65803976-65803995 Msa1031280:intron 50.0%
ACCGGGACATGTCTAATCAG+TGG + chr7_1:65804225-65804244 Msa1031280:intron 50.0%
! CCCACTGATTAGACATGTCC+CGG - chr7_1:65804229-65804248 None:intergenic 50.0%
CCGGGACATGTCTAATCAGT+GGG + chr7_1:65804226-65804245 Msa1031280:intron 50.0%
CGGGACATGTCTAATCAGTG+GGG + chr7_1:65804227-65804246 Msa1031280:intron 50.0%
TCCTACTTGACGTGGGACTT+AGG - chr7_1:65804759-65804778 None:intergenic 50.0%
GCATCTCATCCTACTTGACG+TGG - chr7_1:65804767-65804786 None:intergenic 50.0%
TGATAAGCACCCCAAGCACT+TGG - chr7_1:65804853-65804872 None:intergenic 50.0%
AGGGCATACCAAAGGAACCA+TGG - chr7_1:65805319-65805338 None:intergenic 50.0%
CTTCGTGCTGTAAAAAGGGC+AGG + chr7_1:65805369-65805388 Msa1031280:CDS 50.0%
TAAACGAAAAGCTGCGCGCA+AGG + chr7_1:65805470-65805489 Msa1031280:CDS 50.0%
TGCGACTCTGTATCCTTACC+CGG + chr7_1:65805718-65805737 Msa1031280:three_prime_UTR 50.0%
!! GAGTCTTCCATTGGTGGTGG+TGG - chr7_1:65803190-65803209 None:intergenic 55.0%
!! GTCATCGGCGCGATTTGTTG+CGG - chr7_1:65803226-65803245 None:intergenic 55.0%
! ATCGCGCCGATGACAATCGA+CGG + chr7_1:65803232-65803251 Msa1031280:CDS 55.0%
! TCGGCACCGTCGATTGTCAT+CGG - chr7_1:65803241-65803260 None:intergenic 55.0%
AGCGAGGGCTAAGTCTTCGT+CGG - chr7_1:65803260-65803279 None:intergenic 55.0%
GAGAGAGTTGTCTGACACGG+CGG - chr7_1:65803327-65803346 None:intergenic 55.0%
CAACTCTCTCGCTGTTACCG+AGG + chr7_1:65803337-65803356 Msa1031280:CDS 55.0%
ACCGACAAAGACACGGACAC+CGG + chr7_1:65803983-65804002 Msa1031280:intron 55.0%
TCCGGTGTCCGTGTCTTTGT+CGG - chr7_1:65803987-65804006 None:intergenic 55.0%
ACGTGATGGCATGTCGGTGT+CGG + chr7_1:65804200-65804219 Msa1031280:intron 55.0%
GCCTAAGTCCCACGTCAAGT+AGG + chr7_1:65804755-65804774 Msa1031280:intron 55.0%
GCACAGTATATAGGGAGGGC+AGG + chr7_1:65805575-65805594 Msa1031280:three_prime_UTR 55.0%
!! ATCGGCGCGATTTGTTGCGG+TGG - chr7_1:65803223-65803242 None:intergenic 60.0%
!! GACCTCGCGGCGGGTTAAAA+CGG - chr7_1:65803290-65803309 None:intergenic 60.0%
AGAGTTGTCTGACACGGCGG+AGG - chr7_1:65803324-65803343 None:intergenic 60.0%
TTATCGTGGTTGCACCGCCG+TGG + chr7_1:65803603-65803622 Msa1031280:CDS 60.0%
GTTGCACCGCCGTGGCAAAA+AGG + chr7_1:65803611-65803630 Msa1031280:CDS 60.0%
! GTGGGGCGTCTGTGCTTCAT+AGG + chr7_1:65804244-65804263 Msa1031280:intron 60.0%
!! CTCGCGGCGGGTTAAAACGG+AGG - chr7_1:65803287-65803306 None:intergenic 65.0%
!! CTCCGTTTTAACCCGCCGCG+AGG + chr7_1:65803285-65803304 Msa1031280:CDS 65.0%
GTTGTCTGACACGGCGGAGG+CGG - chr7_1:65803321-65803340 None:intergenic 65.0%
!! GGCATGTCGGTGTCGGACAC+CGG + chr7_1:65804207-65804226 Msa1031280:intron 65.0%
!! GCATGTCGGTGTCGGACACC+GGG + chr7_1:65804208-65804227 Msa1031280:intron 65.0%
GTCTGACACGGCGGAGGCGG+CGG - chr7_1:65803318-65803337 None:intergenic 75.0%
! GCGGACGAGGACCTCGCGGC+GGG - chr7_1:65803299-65803318 None:intergenic 80.0%
! GGCGGACGAGGACCTCGCGG+CGG - chr7_1:65803300-65803319 None:intergenic 80.0%
! GGCGGCGGACGAGGACCTCG+CGG - chr7_1:65803303-65803322 None:intergenic 80.0%
! CACGGCGGAGGCGGCGGACG+AGG - chr7_1:65803312-65803331 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 65803103 65805848 65803103 ID=Msa1031280;Name=Msa1031280
chr7_1 mRNA 65803103 65805848 65803103 ID=Msa1031280-mRNA-1;Parent=Msa1031280;Name=Msa1031280-mRNA-1;_AED=0.00;_eAED=0.00;_QI=96|1|1|1|1|1|2|327|227
chr7_1 exon 65803103 65803632 65803103 ID=Msa1031280-mRNA-1:exon:14660;Parent=Msa1031280-mRNA-1
chr7_1 exon 65805272 65805848 65805272 ID=Msa1031280-mRNA-1:exon:14661;Parent=Msa1031280-mRNA-1
chr7_1 five_prime_UTR 65803103 65803198 65803103 ID=Msa1031280-mRNA-1:five_prime_utr;Parent=Msa1031280-mRNA-1
chr7_1 CDS 65803199 65803632 65803199 ID=Msa1031280-mRNA-1:cds;Parent=Msa1031280-mRNA-1
chr7_1 CDS 65805272 65805521 65805272 ID=Msa1031280-mRNA-1:cds;Parent=Msa1031280-mRNA-1
chr7_1 three_prime_UTR 65805522 65805848 65805522 ID=Msa1031280-mRNA-1:three_prime_utr;Parent=Msa1031280-mRNA-1
Gene Sequence

>Msa1031280

ATGGAAGACTCTCAACAGCAACCACCGCAACAAATCGCGCCGATGACAATCGACGGTGCCGACGAAGACTTAGCCCTCGCTAACTCCTCCGTTTTAACCCGCCGCGAGGTCCTCGTCCGCCGCCTCCGCCGTGTCAGACAACTCTCTCGCTGTTACCGAGGTCATTACTGGGCGTTAATGGAAGAGCTTAAGGCTAAGTACAGAGAGTATTACTGGACTTATGGAAGGAGTCCGTTCAAGGAAGATGAAGAAAGAAATTCGGGTAACGCTTCTGCTGCTGCTGCTGCTGCTGTTGATAATGGTGATGACTTTGTTCGGTGTGCTTCTAGTGGTTGCAAGACCAAGGCTATGGCTCTTACTAAGTATTGTCATGCGCATATTTTGTCTGATCCTAATCAGAAGCTTTATCGTGGTTGCACCGCCGTGGCAAAAAGTTTGCCAACAGGGCCAGTCTTTTGTAATAAGCCAGTATTGAGATCCATGGTTCCTTTGGTATGCCCTGCTCATTACCAATTAGGCGAAAAATGCTTGCTTCGTGCTGTAAAAAGGGCAGGTCATAACGTCCCAGTTAATGGTAAGCCTAGTCCGCAGTTACATCTATTAATTCCTGAATTTGTCCGCCAAATTCAAAATAAACGAAAAGCTGCGCGCAAGGCAGCTTTACCTAAACCTGAGACTGATTAA
Protein sequence

>Msa1031280

MEDSQQQPPQQIAPMTIDGADEDLALANSSVLTRREVLVRRLRRVRQLSRCYRGHYWALMEELKAKYREYYWTYGRSPFKEDEERNSGNASAAAAAAVDNGDDFVRCASSGCKTKAMALTKYCHAHILSDPNQKLYRGCTAVAKSLPTGPVFCNKPVLRSMVPLVCPAHYQLGEKCLLRAVKRAGHNVPVNGKPSPQLHLLIPEFVRQIQNKRKAARKAALPKPETD*