AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1031540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1031540 MtrunA17_Chr7g0253141 99.620 263 1 0 1 263 1 263 0.0 553
Msa1031540 MtrunA17_Chr1g0169261 30.556 252 154 5 9 256 3 237 9.55e-32 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1031540 AT1G54390.4 78.707 263 51 4 1 261 1 260 5.20e-143 402
Msa1031540 AT1G54390.3 78.707 263 51 4 1 261 1 260 5.20e-143 402
Msa1031540 AT1G54390.7 75.111 225 51 4 39 261 19 240 7.17e-114 327
Msa1031540 AT1G54390.8 75.111 225 51 4 39 261 19 240 7.17e-114 327
Msa1031540 AT1G54390.5 75.111 225 51 4 39 261 19 240 7.17e-114 327
Msa1031540 AT1G54390.1 78.603 229 44 4 1 227 1 226 3.08e-113 328
Msa1031540 AT1G54390.2 77.729 229 46 4 1 227 1 226 3.90e-113 328
Msa1031540 AT1G54390.6 81.768 181 29 3 83 261 1 179 1.09e-99 289
Msa1031540 AT3G24010.1 31.452 248 144 6 9 256 3 224 7.87e-30 112

Find 90 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAATTTCAGGTATCTTT+TGG 0.176968 7_1:+66266063 Msa1031540:intron
ATTGCAATAGAAGAAATATT+AGG 0.220698 7_1:-66264049 None:intergenic
GTTGGGTTATGATTAATTAA+TGG 0.231604 7_1:+66266420 Msa1031540:three_prime_UTR
GAACCAGCAAGAACACTTCT+AGG 0.288960 7_1:+66264003 Msa1031540:five_prime_UTR
TGAACCTTGTCTCTTGTGTT+AGG 0.290251 7_1:-66266258 None:intergenic
TAGTCCTTCTTATGGCTTCC+TGG 0.301453 7_1:-66265869 None:intergenic
TTGCAATAGAAGAAATATTA+GGG 0.310592 7_1:-66264048 None:intergenic
TTGGGTTATGATTAATTAAT+GGG 0.310648 7_1:+66266421 Msa1031540:three_prime_UTR
AGTCTACGTCGATGACTATT+TGG 0.315508 7_1:+66264107 Msa1031540:CDS
CAATAATCATTCAATCAAAA+TGG 0.316662 7_1:+66264068 Msa1031540:exon
AATCGAAGAGACTTGATTAC+AGG 0.362393 7_1:+66265807 Msa1031540:CDS
ACACAGTATGTAGGTTCATT+TGG 0.368463 7_1:-66265926 None:intergenic
ACTTTGTCTGCTGCCTTGTC+TGG 0.368542 7_1:-66264470 None:intergenic
TGTAGGTTCATTTGGATCAA+TGG 0.378225 7_1:-66265918 None:intergenic
AAAAGAAATTGAGGCAAATC+AGG 0.389795 7_1:+66264593 Msa1031540:CDS
GGATCATATAGTCCTTCTTA+TGG 0.391014 7_1:-66265877 None:intergenic
AACCCTAGAAGTGTTCTTGC+TGG 0.406185 7_1:-66264006 None:intergenic
ACTTATGAACTTAGTTCAAC+AGG 0.416135 7_1:-66266343 None:intergenic
GTAGGTTCATTTGGATCAAT+GGG 0.417965 7_1:-66265917 None:intergenic
CTTTAAATCTTCAGCAAAGT+AGG 0.418687 7_1:-66265297 None:intergenic
AAATATTAGGGTTTGCGATT+GGG 0.421821 7_1:-66264036 None:intergenic
TACATACTGTGTTTGTCATC+AGG 0.423437 7_1:+66265937 Msa1031540:CDS
GAAATATTAGGGTTTGCGAT+TGG 0.424049 7_1:-66264037 None:intergenic
CAAAACAACGTAAATATTCC+AGG 0.431982 7_1:+66263937 None:intergenic
TTGATTACAGGGAGAGAGAC+TGG 0.434207 7_1:+66265819 Msa1031540:CDS
GAAAAGCGCAGGCACGTATA+TGG 0.434521 7_1:+66265776 Msa1031540:CDS
TTCTTATGGCTTCCTGGTGG+AGG 0.438248 7_1:-66265863 None:intergenic
TGTCACCGCATGGTGTGAAC+CGG 0.451384 7_1:-66266548 None:intergenic
AAGCTCAGCGGGCAATGTAT+TGG 0.453107 7_1:-66264230 None:intergenic
GCAGCAGACAAAGTACTGTA+TGG 0.463935 7_1:+66264479 Msa1031540:CDS
AAAACGCTGTCGTTTGAGCC+TGG 0.464707 7_1:-66263955 None:intergenic
CTCATCTCATGGCTCTAAGA+AGG 0.470707 7_1:+66264506 Msa1031540:CDS
AACTAACCAGTTCATATGCT+TGG 0.476561 7_1:-66264659 None:intergenic
GTTTAGGGACAATAGGCAAT+GGG 0.494080 7_1:-66265751 None:intergenic
AGAAGTCTCTGAAGCTCAGC+GGG 0.494940 7_1:-66264241 None:intergenic
TTTGCTGAAGATTTAAAGCA+AGG 0.503317 7_1:+66265302 Msa1031540:CDS
GCGCGCCAACAAAACCTTCT+CGG 0.503510 7_1:-66264637 None:intergenic
GGCGCGCCAAGCATATGAAC+TGG 0.511810 7_1:+66264653 Msa1031540:CDS
TGTATGGGGTTCTCATCTCA+TGG 0.513570 7_1:+66264495 Msa1031540:CDS
AACACAAGAGACAAGGTTCA+AGG 0.514404 7_1:+66266261 Msa1031540:CDS
TTGCTTGACTATTTGTGTGC+CGG 0.515421 7_1:+66266529 Msa1031540:three_prime_UTR
GAAGGACTATATGATCCCTA+TGG 0.521889 7_1:+66265883 Msa1031540:CDS
CAGCAGACAAAGTACTGTAT+GGG 0.532458 7_1:+66264480 Msa1031540:CDS
CTGGGATTGGTCTGGTGTGA+GGG 0.532859 7_1:+66266652 Msa1031540:three_prime_UTR
ATGGGTTGATCAATATCCAT+AGG 0.533374 7_1:-66265899 None:intergenic
AAGGGAAAGAGTTACATAAG+AGG 0.534525 7_1:-66266463 None:intergenic
GATAGTGCCGAGGAGGTGAA+TGG 0.537405 7_1:+66266215 Msa1031540:intron
AAAATGGCAATTGCACGCAC+TGG 0.541228 7_1:+66264084 Msa1031540:exon
TAATCAAGTCTCTTCGATTG+AGG 0.541620 7_1:-66265803 None:intergenic
TTAGGCCAACACATGAATAG+TGG 0.541653 7_1:-66266240 None:intergenic
TTTAGGGACAATAGGCAATG+GGG 0.542772 7_1:-66265750 None:intergenic
TCCCTAAACCCGAAAAGCGC+AGG 0.544587 7_1:+66265765 Msa1031540:CDS
TAATAACAATTACATGAATG+AGG 0.550580 7_1:+66264542 Msa1031540:CDS
TCCACCAGGAAGCCATAAGA+AGG 0.551349 7_1:+66265865 Msa1031540:CDS
AAGATTTATCATTGACATTG+TGG 0.551966 7_1:-66266316 None:intergenic
CAGATACGAGGAAGCGAAGG+TGG 0.561608 7_1:-66263981 None:intergenic
TGGTTCCACTATTCATGTGT+TGG 0.563319 7_1:+66266235 Msa1031540:CDS
AGACAAGGTTCAAGGGAAAG+TGG 0.569480 7_1:+66266269 Msa1031540:CDS
ACACAAGAGACAAGGTTCAA+GGG 0.570451 7_1:+66266262 Msa1031540:CDS
TTAGGGACAATAGGCAATGG+GGG 0.570565 7_1:-66265749 None:intergenic
AACCAGCAAGAACACTTCTA+GGG 0.576402 7_1:+66264004 Msa1031540:five_prime_UTR
TCCTTCTTATGGCTTCCTGG+TGG 0.577394 7_1:-66265866 None:intergenic
GAAAATGCGAAAAGAAATTG+AGG 0.579696 7_1:+66264584 Msa1031540:CDS
ATTGAGTTTATGTACCGAGA+AGG 0.582404 7_1:+66264623 Msa1031540:CDS
TGTGGTAGTAATCTGCAAGT+TGG 0.587319 7_1:-66266298 None:intergenic
TTCTTGCTGGTTCAGATACG+AGG 0.590084 7_1:-66263993 None:intergenic
GGTTTAGGGACAATAGGCAA+TGG 0.590587 7_1:-66265752 None:intergenic
TGGGTTGATCAATATCCATA+GGG 0.596146 7_1:-66265898 None:intergenic
TCGTTCATCGAGTTCACGAA+CGG 0.599115 7_1:-66264269 None:intergenic
TCATCTCATGGCTCTAAGAA+GGG 0.599282 7_1:+66264507 Msa1031540:CDS
TGATGACAAACACAGTATGT+AGG 0.600981 7_1:-66265935 None:intergenic
TGGGTTATGATTAATTAATG+GGG 0.608426 7_1:+66266422 Msa1031540:three_prime_UTR
TTTGAGCTAATGCCTCCACC+AGG 0.610776 7_1:+66265851 Msa1031540:CDS
CCATGTAAAACGACTTGATG+AGG 0.613217 7_1:+66265268 Msa1031540:CDS
AAGAAGTCTCTGAAGCTCAG+CGG 0.613708 7_1:-66264242 None:intergenic
AGTGGAACCATTCACCTCCT+CGG 0.620021 7_1:-66266222 None:intergenic
TGATTACAGGGAGAGAGACT+GGG 0.622459 7_1:+66265820 Msa1031540:CDS
CCTTGACTAGAAAATACACG+TGG 0.624932 7_1:+66266482 Msa1031540:three_prime_UTR
ATGTCATCACTGTCACCGCA+TGG 0.625430 7_1:-66266558 None:intergenic
GTTCAGATACGAGGAAGCGA+AGG 0.630829 7_1:-66263984 None:intergenic
ATCGAAGAGACTTGATTACA+GGG 0.633832 7_1:+66265808 Msa1031540:CDS
ACTGGGATTGGTCTGGTGTG+AGG 0.634021 7_1:+66266651 Msa1031540:three_prime_UTR
AAGCTATGATAAACCAGACA+AGG 0.648028 7_1:+66264457 Msa1031540:intron
TATTATTCTTGATAGTGCCG+AGG 0.649316 7_1:+66266205 Msa1031540:intron
TATTCTTGATAGTGCCGAGG+AGG 0.654486 7_1:+66266208 Msa1031540:intron
AGCAGACAAAGTACTGTATG+GGG 0.659495 7_1:+66264481 Msa1031540:CDS
GGATTTGAGTAGTATGCACG+AGG 0.667663 7_1:+66266673 Msa1031540:three_prime_UTR
TTGGCCTAACACAAGAGACA+AGG 0.699970 7_1:+66266254 Msa1031540:CDS
GTGTGCCGGTTCACACCATG+CGG 0.732749 7_1:+66266543 Msa1031540:three_prime_UTR
CTTGACTAGAAAATACACGT+GGG 0.752169 7_1:+66266483 Msa1031540:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAAAAAAGTAAATTTAAT+AGG - chr7_1:66264336-66264355 None:intergenic 10.0%
!!! TAGTTTTTTTTTAACTTTTA+TGG + chr7_1:66264677-66264696 Msa1031540:intron 10.0%
!!! TTTTATATATAGCATTTATA+AGG + chr7_1:66264713-66264732 Msa1031540:intron 10.0%
!! TGGAATAAAATTGAAAAATT+GGG - chr7_1:66264190-66264209 None:intergenic 15.0%
!! TTGGAATAAAATTGAAAAAT+TGG - chr7_1:66264191-66264210 None:intergenic 15.0%
!!! AAAAAAGGAAATTTTTTAGT+AGG - chr7_1:66264309-66264328 None:intergenic 15.0%
!! AATTTAATAGGGTTAAAAAA+AGG - chr7_1:66264324-66264343 None:intergenic 15.0%
!! AGTAATTTAACATCAAAAAA+GGG - chr7_1:66264360-66264379 None:intergenic 15.0%
!! AAAATATTAACAAAAAACAC+TGG + chr7_1:66266633-66266652 Msa1031540:three_prime_UTR 15.0%
!! AAATATTAACAAAAAACACT+GGG + chr7_1:66266634-66266653 Msa1031540:three_prime_UTR 15.0%
!! TTGCAATAGAAGAAATATTA+GGG - chr7_1:66264051-66264070 None:intergenic 20.0%
!! ATTGCAATAGAAGAAATATT+AGG - chr7_1:66264052-66264071 None:intergenic 20.0%
!! CAATAATCATTCAATCAAAA+TGG + chr7_1:66264068-66264087 Msa1031540:exon 20.0%
!! GAATAAAATTGAAAAATTGG+GGG - chr7_1:66264188-66264207 None:intergenic 20.0%
!! GGAATAAAATTGAAAAATTG+GGG - chr7_1:66264189-66264208 None:intergenic 20.0%
!!! TTTTAGTAGGAATTTACATT+GGG - chr7_1:66264296-66264315 None:intergenic 20.0%
!!! TTTTTAGTAGGAATTTACAT+TGG - chr7_1:66264297-66264316 None:intergenic 20.0%
!! TAATTTAACATCAAAAAAGG+GGG - chr7_1:66264358-66264377 None:intergenic 20.0%
!! GTAATTTAACATCAAAAAAG+GGG - chr7_1:66264359-66264378 None:intergenic 20.0%
!! GAGTAATTTAACATCAAAAA+AGG - chr7_1:66264361-66264380 None:intergenic 20.0%
!!! ATCATTATTTTTTGTTGTTG+TGG + chr7_1:66264418-66264437 Msa1031540:intron 20.0%
!! TAATAACAATTACATGAATG+AGG + chr7_1:66264542-66264561 Msa1031540:CDS 20.0%
!!! TTTTTTTAACTTTTATGGCT+TGG + chr7_1:66264682-66264701 Msa1031540:intron 20.0%
!!! ATCAAAAATGAATGTTGTTT+TGG + chr7_1:66265560-66265579 Msa1031540:intron 20.0%
!!! TGTCAATGAAATTGTTTTTT+TGG + chr7_1:66265633-66265652 Msa1031540:intron 20.0%
!!! TTTTAGTGAATGCATAATTT+TGG + chr7_1:66266396-66266415 Msa1031540:three_prime_UTR 20.0%
!!! TTTAGTGAATGCATAATTTT+GGG + chr7_1:66266397-66266416 Msa1031540:three_prime_UTR 20.0%
!! TTGGGTTATGATTAATTAAT+GGG + chr7_1:66266421-66266440 Msa1031540:three_prime_UTR 20.0%
! CGTAGAGAATAGAAAATAAA+AGG - chr7_1:66264143-66264162 None:intergenic 25.0%
! AAAAATTGGGGGAAAAATTA+GGG - chr7_1:66264177-66264196 None:intergenic 25.0%
! CATCTATGGAATAGAAAAAT+TGG - chr7_1:66264210-66264229 None:intergenic 25.0%
! ATAGTAATGAATGACCTTAA+TGG + chr7_1:66264819-66264838 Msa1031540:intron 25.0%
! ACTTAATCACATTACCATTA+AGG - chr7_1:66264836-66264855 None:intergenic 25.0%
! ACATATAATGTAACTGTCTT+CGG - chr7_1:66265055-66265074 None:intergenic 25.0%
!!! AATTTTTTCCATATTGCTTC+TGG + chr7_1:66265171-66265190 Msa1031540:intron 25.0%
!!! GAAGAGTATGTTCAATATTT+TGG + chr7_1:66265461-66265480 Msa1031540:intron 25.0%
!!! TATTTTGGAAGTGCATAATT+TGG + chr7_1:66265476-66265495 Msa1031540:intron 25.0%
!!! TGAATGTTGTTTTGGAATTT+AGG + chr7_1:66265568-66265587 Msa1031540:intron 25.0%
!!! GAATGTTGTTTTGGAATTTA+GGG + chr7_1:66265569-66265588 Msa1031540:intron 25.0%
!!! AATGTTGTTTTGGAATTTAG+GGG + chr7_1:66265570-66265589 Msa1031540:intron 25.0%
! AACAATTTCATTGACAAAGA+AGG - chr7_1:66265629-66265648 None:intergenic 25.0%
! AACTCAGTCAAAATAACAAA+AGG - chr7_1:66266179-66266198 None:intergenic 25.0%
! AAGATTTATCATTGACATTG+TGG - chr7_1:66266319-66266338 None:intergenic 25.0%
!! TTAGTGAATGCATAATTTTG+GGG + chr7_1:66266398-66266417 Msa1031540:three_prime_UTR 25.0%
! GTTGGGTTATGATTAATTAA+TGG + chr7_1:66266420-66266439 Msa1031540:three_prime_UTR 25.0%
! TGGGTTATGATTAATTAATG+GGG + chr7_1:66266422-66266441 Msa1031540:three_prime_UTR 25.0%
AAATATTAGGGTTTGCGATT+GGG - chr7_1:66264039-66264058 None:intergenic 30.0%
GAAAAATTGGGGGAAAAATT+AGG - chr7_1:66264178-66264197 None:intergenic 30.0%
! TTCTTTTCGCATTTTCTCAA+TGG - chr7_1:66264580-66264599 None:intergenic 30.0%
GAAAATGCGAAAAGAAATTG+AGG + chr7_1:66264584-66264603 Msa1031540:CDS 30.0%
AAAAGAAATTGAGGCAAATC+AGG + chr7_1:66264593-66264612 Msa1031540:CDS 30.0%
TGTGAATTATTGTTGCAACA+AGG + chr7_1:66264744-66264763 Msa1031540:intron 30.0%
GTGAATTATTGTTGCAACAA+GGG + chr7_1:66264745-66264764 Msa1031540:intron 30.0%
TGAATTATTGTTGCAACAAG+GGG + chr7_1:66264746-66264765 Msa1031540:intron 30.0%
AGCTTAAACAAGAAGAATGA+TGG - chr7_1:66265009-66265028 None:intergenic 30.0%
!! TTTGCTTTAAGTTGTTTGAG+AGG + chr7_1:66265103-66265122 Msa1031540:intron 30.0%
CTTTAAATCTTCAGCAAAGT+AGG - chr7_1:66265300-66265319 None:intergenic 30.0%
! TTTGCTGAAGATTTAAAGCA+AGG + chr7_1:66265302-66265321 Msa1031540:CDS 30.0%
AAATCAGAATGCAAGTCAAA+TGG - chr7_1:66265336-66265355 None:intergenic 30.0%
CCAGATCATACTAATTGTAT+AGG - chr7_1:66265396-66265415 None:intergenic 30.0%
CCTATACAATTAGTATGATC+TGG + chr7_1:66265393-66265412 Msa1031540:intron 30.0%
CATTACATGTTTCTTTAGAC+AGG - chr7_1:66265512-66265531 None:intergenic 30.0%
!! TTCACATTTGATTTTGTCAG+AGG + chr7_1:66265694-66265713 Msa1031540:intron 30.0%
!! TCACATTTGATTTTGTCAGA+GGG + chr7_1:66265695-66265714 Msa1031540:intron 30.0%
AGTCAGTTTACTGAAATTTC+AGG + chr7_1:66266053-66266072 Msa1031540:intron 30.0%
!! CTGAAATTTCAGGTATCTTT+TGG + chr7_1:66266063-66266082 Msa1031540:intron 30.0%
GTCAAAATAACAAAAGGAGA+GGG - chr7_1:66266173-66266192 None:intergenic 30.0%
AGTCAAAATAACAAAAGGAG+AGG - chr7_1:66266174-66266193 None:intergenic 30.0%
ACTTATGAACTTAGTTCAAC+AGG - chr7_1:66266346-66266365 None:intergenic 30.0%
TTAACAAAAAACACTGGGAT+TGG + chr7_1:66266639-66266658 Msa1031540:three_prime_UTR 30.0%
GAAATATTAGGGTTTGCGAT+TGG - chr7_1:66264040-66264059 None:intergenic 35.0%
AAGCTATGATAAACCAGACA+AGG + chr7_1:66264457-66264476 Msa1031540:intron 35.0%
ATTGAGTTTATGTACCGAGA+AGG + chr7_1:66264623-66264642 Msa1031540:CDS 35.0%
AACTAACCAGTTCATATGCT+TGG - chr7_1:66264662-66264681 None:intergenic 35.0%
!! AACTTTTATGGCTTGGACAA+AGG + chr7_1:66264689-66264708 Msa1031540:intron 35.0%
!! ACTTTTATGGCTTGGACAAA+GGG + chr7_1:66264690-66264709 Msa1031540:intron 35.0%
GTTCAGAAATGTAAATCCCT+AGG - chr7_1:66264772-66264791 None:intergenic 35.0%
! TCACTGTACAATTTTTCTCG+AGG + chr7_1:66264979-66264998 Msa1031540:intron 35.0%
ATTCACAAAACTCCCAAGAT+AGG - chr7_1:66265154-66265173 None:intergenic 35.0%
ATGCATAACCAGAAGCAATA+TGG - chr7_1:66265182-66265201 None:intergenic 35.0%
TCATTGACAAAGAAGGAAGA+GGG - chr7_1:66265622-66265641 None:intergenic 35.0%
TTCATTGACAAAGAAGGAAG+AGG - chr7_1:66265623-66265642 None:intergenic 35.0%
!! CACATTTGATTTTGTCAGAG+GGG + chr7_1:66265696-66265715 Msa1031540:intron 35.0%
TAATCAAGTCTCTTCGATTG+AGG - chr7_1:66265806-66265825 None:intergenic 35.0%
AATCGAAGAGACTTGATTAC+AGG + chr7_1:66265807-66265826 Msa1031540:CDS 35.0%
ATCGAAGAGACTTGATTACA+GGG + chr7_1:66265808-66265827 Msa1031540:CDS 35.0%
GGATCATATAGTCCTTCTTA+TGG - chr7_1:66265880-66265899 None:intergenic 35.0%
!! TGGGTTGATCAATATCCATA+GGG - chr7_1:66265901-66265920 None:intergenic 35.0%
!! ATGGGTTGATCAATATCCAT+AGG - chr7_1:66265902-66265921 None:intergenic 35.0%
GTAGGTTCATTTGGATCAAT+GGG - chr7_1:66265920-66265939 None:intergenic 35.0%
TGTAGGTTCATTTGGATCAA+TGG - chr7_1:66265921-66265940 None:intergenic 35.0%
ACACAGTATGTAGGTTCATT+TGG - chr7_1:66265929-66265948 None:intergenic 35.0%
TGATGACAAACACAGTATGT+AGG - chr7_1:66265938-66265957 None:intergenic 35.0%
TACATACTGTGTTTGTCATC+AGG + chr7_1:66265937-66265956 Msa1031540:CDS 35.0%
! TATAGTTTCAAGTCTCCTTG+TGG + chr7_1:66265991-66266010 Msa1031540:intron 35.0%
ATATGACACAATTGTCCACA+AGG - chr7_1:66266009-66266028 None:intergenic 35.0%
!! TATTATTCTTGATAGTGCCG+AGG + chr7_1:66266205-66266224 Msa1031540:intron 35.0%
!!! TGAATGCATAATTTTGGGGT+TGG + chr7_1:66266402-66266421 Msa1031540:three_prime_UTR 35.0%
!!! GAATGCATAATTTTGGGGTT+GGG + chr7_1:66266403-66266422 Msa1031540:three_prime_UTR 35.0%
AAGGGAAAGAGTTACATAAG+AGG - chr7_1:66266466-66266485 None:intergenic 35.0%
!! CACGTGTATTTTCTAGTCAA+GGG - chr7_1:66266484-66266503 None:intergenic 35.0%
CTTGACTAGAAAATACACGT+GGG + chr7_1:66266483-66266502 Msa1031540:three_prime_UTR 35.0%
AACCAGCAAGAACACTTCTA+GGG + chr7_1:66264004-66264023 Msa1031540:five_prime_UTR 40.0%
AGTCTACGTCGATGACTATT+TGG + chr7_1:66264107-66264126 Msa1031540:CDS 40.0%
TGGGGGAAAAATTAGGGTTA+GGG - chr7_1:66264171-66264190 None:intergenic 40.0%
TTGGGGGAAAAATTAGGGTT+AGG - chr7_1:66264172-66264191 None:intergenic 40.0%
!! CAGCAGACAAAGTACTGTAT+GGG + chr7_1:66264480-66264499 Msa1031540:CDS 40.0%
!! AGCAGACAAAGTACTGTATG+GGG + chr7_1:66264481-66264500 Msa1031540:CDS 40.0%
!! TCATCTCATGGCTCTAAGAA+GGG + chr7_1:66264507-66264526 Msa1031540:CDS 40.0%
!! ATGTACCGAGAAGGTTTTGT+TGG + chr7_1:66264632-66264651 Msa1031540:CDS 40.0%
ATGATTGCAGCTCCTATCTT+GGG + chr7_1:66265139-66265158 Msa1031540:intron 40.0%
!! CCTCATCAAGTCGTTTTACA+TGG - chr7_1:66265271-66265290 None:intergenic 40.0%
CCATGTAAAACGACTTGATG+AGG + chr7_1:66265268-66265287 Msa1031540:CDS 40.0%
CATTGACAAAGAAGGAAGAG+GGG - chr7_1:66265621-66265640 None:intergenic 40.0%
TTTAGGGACAATAGGCAATG+GGG - chr7_1:66265753-66265772 None:intergenic 40.0%
GTTTAGGGACAATAGGCAAT+GGG - chr7_1:66265754-66265773 None:intergenic 40.0%
!!! TTTTCGGGTTTAGGGACAAT+AGG - chr7_1:66265761-66265780 None:intergenic 40.0%
GAAGGACTATATGATCCCTA+TGG + chr7_1:66265883-66265902 Msa1031540:CDS 40.0%
TGGTTCCACTATTCATGTGT+TGG + chr7_1:66266235-66266254 Msa1031540:CDS 40.0%
TTAGGCCAACACATGAATAG+TGG - chr7_1:66266243-66266262 None:intergenic 40.0%
TGAACCTTGTCTCTTGTGTT+AGG - chr7_1:66266261-66266280 None:intergenic 40.0%
AACACAAGAGACAAGGTTCA+AGG + chr7_1:66266261-66266280 Msa1031540:CDS 40.0%
ACACAAGAGACAAGGTTCAA+GGG + chr7_1:66266262-66266281 Msa1031540:CDS 40.0%
TGTGGTAGTAATCTGCAAGT+TGG - chr7_1:66266301-66266320 None:intergenic 40.0%
!! CCACGTGTATTTTCTAGTCA+AGG - chr7_1:66266485-66266504 None:intergenic 40.0%
CCTTGACTAGAAAATACACG+TGG + chr7_1:66266482-66266501 Msa1031540:three_prime_UTR 40.0%
TTGCTTGACTATTTGTGTGC+CGG + chr7_1:66266529-66266548 Msa1031540:three_prime_UTR 40.0%
!! AAAAAACACTGGGATTGGTC+TGG + chr7_1:66266644-66266663 Msa1031540:three_prime_UTR 40.0%
!! TTCTTGCTGGTTCAGATACG+AGG - chr7_1:66263996-66264015 None:intergenic 45.0%
GAACCAGCAAGAACACTTCT+AGG + chr7_1:66264003-66264022 Msa1031540:five_prime_UTR 45.0%
!! AACCCTAGAAGTGTTCTTGC+TGG - chr7_1:66264009-66264028 None:intergenic 45.0%
AAAATGGCAATTGCACGCAC+TGG + chr7_1:66264084-66264103 Msa1031540:exon 45.0%
! GGGCAATGTATTGGCATCTA+TGG - chr7_1:66264224-66264243 None:intergenic 45.0%
!! AAGAAGTCTCTGAAGCTCAG+CGG - chr7_1:66264245-66264264 None:intergenic 45.0%
TCGTTCATCGAGTTCACGAA+CGG - chr7_1:66264272-66264291 None:intergenic 45.0%
!! GCAGCAGACAAAGTACTGTA+TGG + chr7_1:66264479-66264498 Msa1031540:CDS 45.0%
TGTATGGGGTTCTCATCTCA+TGG + chr7_1:66264495-66264514 Msa1031540:CDS 45.0%
!! CTCATCTCATGGCTCTAAGA+AGG + chr7_1:66264506-66264525 Msa1031540:CDS 45.0%
GATGATTGCAGCTCCTATCT+TGG + chr7_1:66265138-66265157 Msa1031540:intron 45.0%
AAGGAAGAGGGGAATTTCAG+GGG - chr7_1:66265610-66265629 None:intergenic 45.0%
GAAGGAAGAGGGGAATTTCA+GGG - chr7_1:66265611-66265630 None:intergenic 45.0%
AGAAGGAAGAGGGGAATTTC+AGG - chr7_1:66265612-66265631 None:intergenic 45.0%
TTAGGGACAATAGGCAATGG+GGG - chr7_1:66265752-66265771 None:intergenic 45.0%
GGTTTAGGGACAATAGGCAA+TGG - chr7_1:66265755-66265774 None:intergenic 45.0%
! ATATACGTGCCTGCGCTTTT+CGG - chr7_1:66265777-66265796 None:intergenic 45.0%
TTGATTACAGGGAGAGAGAC+TGG + chr7_1:66265819-66265838 Msa1031540:CDS 45.0%
TGATTACAGGGAGAGAGACT+GGG + chr7_1:66265820-66265839 Msa1031540:CDS 45.0%
TAGTCCTTCTTATGGCTTCC+TGG - chr7_1:66265872-66265891 None:intergenic 45.0%
TGAACTTTGCTCTCTTGTGC+TGG + chr7_1:66266124-66266143 Msa1031540:intron 45.0%
!! TATTCTTGATAGTGCCGAGG+AGG + chr7_1:66266208-66266227 Msa1031540:intron 45.0%
TTGGCCTAACACAAGAGACA+AGG + chr7_1:66266254-66266273 Msa1031540:CDS 45.0%
AGACAAGGTTCAAGGGAAAG+TGG + chr7_1:66266269-66266288 Msa1031540:CDS 45.0%
GGATTTGAGTAGTATGCACG+AGG + chr7_1:66266673-66266692 Msa1031540:three_prime_UTR 45.0%
!! TAAAAAAAGTAAATTTAATA+GGG - chr7_1:66264335-66264354 None:intergenic 5.0%
!! AAAAAAAAAAAAAATAAGAA+AGG - chr7_1:66265676-66265695 None:intergenic 5.0%
! AAAACGCTGTCGTTTGAGCC+TGG - chr7_1:66263958-66263977 None:intergenic 50.0%
GTTCAGATACGAGGAAGCGA+AGG - chr7_1:66263987-66264006 None:intergenic 50.0%
AAGCTCAGCGGGCAATGTAT+TGG - chr7_1:66264233-66264252 None:intergenic 50.0%
!! AGAAGTCTCTGAAGCTCAGC+GGG - chr7_1:66264244-66264263 None:intergenic 50.0%
ACTTTGTCTGCTGCCTTGTC+TGG - chr7_1:66264473-66264492 None:intergenic 50.0%
ATTGTTGCAACAAGGGGCCT+AGG + chr7_1:66264752-66264771 Msa1031540:intron 50.0%
TTGTTGCAACAAGGGGCCTA+GGG + chr7_1:66264753-66264772 Msa1031540:intron 50.0%
! TATACGTGCCTGCGCTTTTC+GGG - chr7_1:66265776-66265795 None:intergenic 50.0%
! GAAAAGCGCAGGCACGTATA+TGG + chr7_1:66265776-66265795 Msa1031540:CDS 50.0%
TTTGAGCTAATGCCTCCACC+AGG + chr7_1:66265851-66265870 Msa1031540:CDS 50.0%
TTCTTATGGCTTCCTGGTGG+AGG - chr7_1:66265866-66265885 None:intergenic 50.0%
TCCACCAGGAAGCCATAAGA+AGG + chr7_1:66265865-66265884 Msa1031540:CDS 50.0%
TCCTTCTTATGGCTTCCTGG+TGG - chr7_1:66265869-66265888 None:intergenic 50.0%
AGTGGAACCATTCACCTCCT+CGG - chr7_1:66266225-66266244 None:intergenic 50.0%
ATGTCATCACTGTCACCGCA+TGG - chr7_1:66266561-66266580 None:intergenic 50.0%
CAGATACGAGGAAGCGAAGG+TGG - chr7_1:66263984-66264003 None:intergenic 55.0%
GCGCGCCAACAAAACCTTCT+CGG - chr7_1:66264640-66264659 None:intergenic 55.0%
GGCAATGGGGGAAGAATTGC+TGG - chr7_1:66265740-66265759 None:intergenic 55.0%
! TCCCTAAACCCGAAAAGCGC+AGG + chr7_1:66265765-66265784 Msa1031540:CDS 55.0%
!!! GCCTGCGCTTTTCGGGTTTA+GGG - chr7_1:66265769-66265788 None:intergenic 55.0%
!!! TGCCTGCGCTTTTCGGGTTT+AGG - chr7_1:66265770-66265789 None:intergenic 55.0%
! GATAGTGCCGAGGAGGTGAA+TGG + chr7_1:66266215-66266234 Msa1031540:intron 55.0%
TGTCACCGCATGGTGTGAAC+CGG - chr7_1:66266551-66266570 None:intergenic 55.0%
!! ACTGGGATTGGTCTGGTGTG+AGG + chr7_1:66266651-66266670 Msa1031540:three_prime_UTR 55.0%
!! CTGGGATTGGTCTGGTGTGA+GGG + chr7_1:66266652-66266671 Msa1031540:three_prime_UTR 55.0%
GGCGCGCCAAGCATATGAAC+TGG + chr7_1:66264653-66264672 Msa1031540:CDS 60.0%
!! GTGTGCCGGTTCACACCATG+CGG + chr7_1:66266543-66266562 Msa1031540:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 66263939 66266706 66263939 ID=Msa1031540;Name=Msa1031540
chr7_1 mRNA 66263939 66266706 66263939 ID=Msa1031540-mRNA-1;Parent=Msa1031540;Name=Msa1031540-mRNA-1;_AED=0.19;_eAED=0.19;_QI=148|1|1|1|1|1|7|376|263
chr7_1 exon 66263939 66264132 66263939 ID=Msa1031540-mRNA-1:exon:14842;Parent=Msa1031540-mRNA-1
chr7_1 exon 66264227 66264298 66264227 ID=Msa1031540-mRNA-1:exon:14843;Parent=Msa1031540-mRNA-1
chr7_1 exon 66264460 66264674 66264460 ID=Msa1031540-mRNA-1:exon:14844;Parent=Msa1031540-mRNA-1
chr7_1 exon 66265260 66265323 66265260 ID=Msa1031540-mRNA-1:exon:14845;Parent=Msa1031540-mRNA-1
chr7_1 exon 66265714 66265958 66265714 ID=Msa1031540-mRNA-1:exon:14846;Parent=Msa1031540-mRNA-1
chr7_1 exon 66266075 66266113 66266075 ID=Msa1031540-mRNA-1:exon:14847;Parent=Msa1031540-mRNA-1
chr7_1 exon 66266220 66266706 66266220 ID=Msa1031540-mRNA-1:exon:14848;Parent=Msa1031540-mRNA-1
chr7_1 five_prime_UTR 66263939 66264086 66263939 ID=Msa1031540-mRNA-1:five_prime_utr;Parent=Msa1031540-mRNA-1
chr7_1 CDS 66264087 66264132 66264087 ID=Msa1031540-mRNA-1:cds;Parent=Msa1031540-mRNA-1
chr7_1 CDS 66264227 66264298 66264227 ID=Msa1031540-mRNA-1:cds;Parent=Msa1031540-mRNA-1
chr7_1 CDS 66264460 66264674 66264460 ID=Msa1031540-mRNA-1:cds;Parent=Msa1031540-mRNA-1
chr7_1 CDS 66265260 66265323 66265260 ID=Msa1031540-mRNA-1:cds;Parent=Msa1031540-mRNA-1
chr7_1 CDS 66265714 66265958 66265714 ID=Msa1031540-mRNA-1:cds;Parent=Msa1031540-mRNA-1
chr7_1 CDS 66266075 66266113 66266075 ID=Msa1031540-mRNA-1:cds;Parent=Msa1031540-mRNA-1
chr7_1 CDS 66266220 66266330 66266220 ID=Msa1031540-mRNA-1:cds;Parent=Msa1031540-mRNA-1
chr7_1 three_prime_UTR 66266331 66266706 66266331 ID=Msa1031540-mRNA-1:three_prime_utr;Parent=Msa1031540-mRNA-1
Gene Sequence

>Msa1031540

ATGGCAATTGCACGCACTGGAGTCTACGTCGATGACTATTTGGAGTATGCCAATACATTGCCCGCTGAGCTTCAGAGACTTCTTAACACCGTTCGTGAACTCGATGAACGATCCCAATCTATGATAAACCAGACAAGGCAGCAGACAAAGTACTGTATGGGGTTCTCATCTCATGGCTCTAAGAAGGGTAATCATAATTATAATAACAATTACATGAATGAGGATGATGATGCTTCCATTGAGAAAATGCGAAAAGAAATTGAGGCAAATCAGGACAGTGCATTGAGTTTATGTACCGAGAAGGTTTTGTTGGCGCGCCAAGCATATGAACTGATAGACAGCCATGTAAAACGACTTGATGAGGATTTAACCTACTTTGCTGAAGATTTAAAGCAAGAGGGGAAAATATCACAAGATGAGCCAGCAATTCTTCCCCCATTGCCTATTGTCCCTAAACCCGAAAAGCGCAGGCACGTATATGGTACACCTCAATCGAAGAGACTTGATTACAGGGAGAGAGACTGGGATAGAGACTTTGAGCTAATGCCTCCACCAGGAAGCCATAAGAAGGACTATATGATCCCTATGGATATTGATCAACCCATTGATCCAAATGAACCTACATACTGTGTTTGTCATCAGGTATCTTTTGGAGATATGATTGCTTGTGACAACGAAAATTGCCGAGGAGGTGAATGGTTCCACTATTCATGTGTTGGCCTAACACAAGAGACAAGGTTCAAGGGAAAGTGGTATTGTCCAACTTGCAGATTACTACCACAATGTCAATGA
Protein sequence

>Msa1031540

MAIARTGVYVDDYLEYANTLPAELQRLLNTVRELDERSQSMINQTRQQTKYCMGFSSHGSKKGNHNYNNNYMNEDDDASIEKMRKEIEANQDSALSLCTEKVLLARQAYELIDSHVKRLDEDLTYFAEDLKQEGKISQDEPAILPPLPIVPKPEKRRHVYGTPQSKRLDYRERDWDRDFELMPPPGSHKKDYMIPMDIDQPIDPNEPTYCVCHQVSFGDMIACDNENCRGGEWFHYSCVGLTQETRFKGKWYCPTCRLLPQCQ*