AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1101310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1101310 MtrunA17_Chr7g0253141 99.620 263 1 0 1 263 1 263 0.0 553
Msa1101310 MtrunA17_Chr1g0169261 30.556 252 154 5 9 256 3 237 9.55e-32 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1101310 AT1G54390.4 78.707 263 51 4 1 261 1 260 5.20e-143 402
Msa1101310 AT1G54390.3 78.707 263 51 4 1 261 1 260 5.20e-143 402
Msa1101310 AT1G54390.7 75.111 225 51 4 39 261 19 240 7.17e-114 327
Msa1101310 AT1G54390.8 75.111 225 51 4 39 261 19 240 7.17e-114 327
Msa1101310 AT1G54390.5 75.111 225 51 4 39 261 19 240 7.17e-114 327
Msa1101310 AT1G54390.1 78.603 229 44 4 1 227 1 226 3.08e-113 328
Msa1101310 AT1G54390.2 77.729 229 46 4 1 227 1 226 3.90e-113 328
Msa1101310 AT1G54390.6 81.768 181 29 3 83 261 1 179 1.09e-99 289
Msa1101310 AT3G24010.1 31.452 248 144 6 9 256 3 224 7.87e-30 112

Find 90 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAATTTCAGGTATCTTT+TGG 0.176968 7_3:+32023412 Msa1101310:intron
ATTGCAATAGAAGAAATATT+AGG 0.220698 7_3:-32021391 None:intergenic
GTTGGGTTATGATTAATTAA+TGG 0.231604 7_3:+32023769 Msa1101310:three_prime_UTR
GAACCAGCAAGAACACTTCT+AGG 0.288960 7_3:+32021345 Msa1101310:five_prime_UTR
TGAACCTTGTCTCTTGTGTT+AGG 0.290251 7_3:-32023607 None:intergenic
TAGTCCTTCTTATGGCTTCC+TGG 0.301453 7_3:-32023218 None:intergenic
TTGCAATAGAAGAAATATTA+GGG 0.310592 7_3:-32021390 None:intergenic
TTGGGTTATGATTAATTAAT+GGG 0.310648 7_3:+32023770 Msa1101310:three_prime_UTR
AGTCTACGTCGATGACTATT+TGG 0.315508 7_3:+32021449 Msa1101310:CDS
CAATAATCATTCAATCAAAA+TGG 0.316662 7_3:+32021410 Msa1101310:exon
AATCGAAGAGACTTGATTAC+AGG 0.362393 7_3:+32023156 Msa1101310:CDS
ACACAGTATGTAGGTTCATT+TGG 0.368463 7_3:-32023275 None:intergenic
ACTTTGTCTGCTGCCTTGTC+TGG 0.368542 7_3:-32021812 None:intergenic
TGTAGGTTCATTTGGATCAA+TGG 0.378225 7_3:-32023267 None:intergenic
AAAAGAAATTGAGGCAAATC+AGG 0.389795 7_3:+32021935 Msa1101310:CDS
GGATCATATAGTCCTTCTTA+TGG 0.391014 7_3:-32023226 None:intergenic
AACCCTAGAAGTGTTCTTGC+TGG 0.406185 7_3:-32021348 None:intergenic
ACTTATGAACTTAGTTCAAC+AGG 0.416135 7_3:-32023692 None:intergenic
GTAGGTTCATTTGGATCAAT+GGG 0.417965 7_3:-32023266 None:intergenic
CTTTAAATCTTCAGCAAAGT+AGG 0.418687 7_3:-32022640 None:intergenic
AAATATTAGGGTTTGCGATT+GGG 0.421821 7_3:-32021378 None:intergenic
TACATACTGTGTTTGTCATC+AGG 0.423437 7_3:+32023286 Msa1101310:CDS
GAAATATTAGGGTTTGCGAT+TGG 0.424049 7_3:-32021379 None:intergenic
CAAAACAACGTAAATATTCC+AGG 0.431982 7_3:+32021279 None:intergenic
TTGATTACAGGGAGAGAGAC+TGG 0.434207 7_3:+32023168 Msa1101310:CDS
GAAAAGCGCAGGCACGTATA+TGG 0.434521 7_3:+32023125 Msa1101310:CDS
TTCTTATGGCTTCCTGGTGG+AGG 0.438248 7_3:-32023212 None:intergenic
TGTCACCGCATGGTGTGAAC+CGG 0.451384 7_3:-32023897 None:intergenic
AAGCTCAGCGGGCAATGTAT+TGG 0.453107 7_3:-32021572 None:intergenic
GCAGCAGACAAAGTACTGTA+TGG 0.463935 7_3:+32021821 Msa1101310:CDS
AAAACGCTGTCGTTTGAGCC+TGG 0.464707 7_3:-32021297 None:intergenic
CTCATCTCATGGCTCTAAGA+AGG 0.470707 7_3:+32021848 Msa1101310:CDS
AACTAACCAGTTCATATGCT+TGG 0.476561 7_3:-32022001 None:intergenic
GTTTAGGGACAATAGGCAAT+GGG 0.494080 7_3:-32023100 None:intergenic
AGAAGTCTCTGAAGCTCAGC+GGG 0.494940 7_3:-32021583 None:intergenic
TTTGCTGAAGATTTAAAGCA+AGG 0.503317 7_3:+32022645 Msa1101310:CDS
GCGCGCCAACAAAACCTTCT+CGG 0.503510 7_3:-32021979 None:intergenic
TTGCTCGACTATTTGTGTGC+CGG 0.505173 7_3:+32023878 Msa1101310:three_prime_UTR
GGCGCGCCAAGCATATGAAC+TGG 0.511810 7_3:+32021995 Msa1101310:CDS
TGTATGGGGTTCTCATCTCA+TGG 0.513570 7_3:+32021837 Msa1101310:CDS
AACACAAGAGACAAGGTTCA+AGG 0.514404 7_3:+32023610 Msa1101310:CDS
GAAGGACTATATGATCCCTA+TGG 0.521889 7_3:+32023232 Msa1101310:CDS
CAGCAGACAAAGTACTGTAT+GGG 0.532458 7_3:+32021822 Msa1101310:CDS
CTGGGATTGGTCTGGTGTGA+GGG 0.532859 7_3:+32024001 Msa1101310:three_prime_UTR
ATGGGTTGATCAATATCCAT+AGG 0.533374 7_3:-32023248 None:intergenic
AAGGGAAAGAGTTACATAAG+AGG 0.534525 7_3:-32023812 None:intergenic
GATAGTGCCGAGGAGGTGAA+TGG 0.537405 7_3:+32023564 Msa1101310:intron
AAAATGGCAATTGCACGCAC+TGG 0.541228 7_3:+32021426 Msa1101310:exon
TAATCAAGTCTCTTCGATTG+AGG 0.541620 7_3:-32023152 None:intergenic
TTAGGCCAACACATGAATAG+TGG 0.541653 7_3:-32023589 None:intergenic
TTTAGGGACAATAGGCAATG+GGG 0.542772 7_3:-32023099 None:intergenic
TCCCTAAACCCGAAAAGCGC+AGG 0.544587 7_3:+32023114 Msa1101310:CDS
TAATAACAATTACATGAATG+AGG 0.550580 7_3:+32021884 Msa1101310:CDS
TCCACCAGGAAGCCATAAGA+AGG 0.551349 7_3:+32023214 Msa1101310:CDS
AAGATTTATCATTGACATTG+TGG 0.551966 7_3:-32023665 None:intergenic
CAGATACGAGGAAGCGAAGG+TGG 0.561608 7_3:-32021323 None:intergenic
TGGTTCCACTATTCATGTGT+TGG 0.563319 7_3:+32023584 Msa1101310:CDS
AGACAAGGTTCAAGGGAAAG+TGG 0.569480 7_3:+32023618 Msa1101310:CDS
ACACAAGAGACAAGGTTCAA+GGG 0.570451 7_3:+32023611 Msa1101310:CDS
TTAGGGACAATAGGCAATGG+GGG 0.570565 7_3:-32023098 None:intergenic
AACCAGCAAGAACACTTCTA+GGG 0.576402 7_3:+32021346 Msa1101310:five_prime_UTR
TCCTTCTTATGGCTTCCTGG+TGG 0.577394 7_3:-32023215 None:intergenic
GAAAATGCGAAAAGAAATTG+AGG 0.579696 7_3:+32021926 Msa1101310:CDS
ATTGAGTTTATGTACCGAGA+AGG 0.582404 7_3:+32021965 Msa1101310:CDS
TGTGGTAGTAATCTGCAAGT+TGG 0.587319 7_3:-32023647 None:intergenic
TTCTTGCTGGTTCAGATACG+AGG 0.590084 7_3:-32021335 None:intergenic
GGTTTAGGGACAATAGGCAA+TGG 0.590587 7_3:-32023101 None:intergenic
TGGGTTGATCAATATCCATA+GGG 0.596146 7_3:-32023247 None:intergenic
TCGTTCATCGAGTTCACGAA+CGG 0.599115 7_3:-32021611 None:intergenic
TCATCTCATGGCTCTAAGAA+GGG 0.599282 7_3:+32021849 Msa1101310:CDS
TGATGACAAACACAGTATGT+AGG 0.600981 7_3:-32023284 None:intergenic
TGGGTTATGATTAATTAATG+GGG 0.608426 7_3:+32023771 Msa1101310:three_prime_UTR
TTTGAGCTAATGCCTCCACC+AGG 0.610776 7_3:+32023200 Msa1101310:CDS
CCATGTAAAACGACTTGATG+AGG 0.613217 7_3:+32022611 Msa1101310:CDS
AAGAAGTCTCTGAAGCTCAG+CGG 0.613708 7_3:-32021584 None:intergenic
AGTGGAACCATTCACCTCCT+CGG 0.620021 7_3:-32023571 None:intergenic
TGATTACAGGGAGAGAGACT+GGG 0.622459 7_3:+32023169 Msa1101310:CDS
CCTTGACTAGAAAATACACG+TGG 0.624932 7_3:+32023831 Msa1101310:three_prime_UTR
ATGTCATCACTGTCACCGCA+TGG 0.625430 7_3:-32023907 None:intergenic
GTTCAGATACGAGGAAGCGA+AGG 0.630829 7_3:-32021326 None:intergenic
ATCGAAGAGACTTGATTACA+GGG 0.633832 7_3:+32023157 Msa1101310:CDS
ACTGGGATTGGTCTGGTGTG+AGG 0.634021 7_3:+32024000 Msa1101310:three_prime_UTR
AAGCTATGATAAACCAGACA+AGG 0.648028 7_3:+32021799 Msa1101310:intron
TATTATTCTTGATAGTGCCG+AGG 0.649316 7_3:+32023554 Msa1101310:intron
TATTCTTGATAGTGCCGAGG+AGG 0.654486 7_3:+32023557 Msa1101310:intron
AGCAGACAAAGTACTGTATG+GGG 0.659495 7_3:+32021823 Msa1101310:CDS
GGATTTGAGTAGTATGCACG+AGG 0.667663 7_3:+32024022 Msa1101310:three_prime_UTR
TTGGCCTAACACAAGAGACA+AGG 0.699970 7_3:+32023603 Msa1101310:CDS
GTGTGCCGGTTCACACCATG+CGG 0.732749 7_3:+32023892 Msa1101310:three_prime_UTR
CTTGACTAGAAAATACACGT+GGG 0.752169 7_3:+32023832 Msa1101310:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAAAAAAGTAAATTTAAT+AGG - chr7_3:32021678-32021697 None:intergenic 10.0%
!!! AGTTTTTTTTTAAACTTTTA+TGG + chr7_3:32022020-32022039 Msa1101310:intron 10.0%
!!! TTTTATATATAGCATTTATA+AGG + chr7_3:32022056-32022075 Msa1101310:intron 10.0%
!! TGGAATAAAATTGAAAAATT+GGG - chr7_3:32021532-32021551 None:intergenic 15.0%
!! TTGGAATAAAATTGAAAAAT+TGG - chr7_3:32021533-32021552 None:intergenic 15.0%
!!! AAAAAAGGAAATTTTTTAGT+AGG - chr7_3:32021651-32021670 None:intergenic 15.0%
!! AATTTAATAGGGTTAAAAAA+AGG - chr7_3:32021666-32021685 None:intergenic 15.0%
!! AGTAATTTAACATCAAAAAA+GGG - chr7_3:32021702-32021721 None:intergenic 15.0%
!! AAAATATTAACAAAAAACAC+TGG + chr7_3:32023982-32024001 Msa1101310:three_prime_UTR 15.0%
!! AAATATTAACAAAAAACACT+GGG + chr7_3:32023983-32024002 Msa1101310:three_prime_UTR 15.0%
!! TTGCAATAGAAGAAATATTA+GGG - chr7_3:32021393-32021412 None:intergenic 20.0%
!! ATTGCAATAGAAGAAATATT+AGG - chr7_3:32021394-32021413 None:intergenic 20.0%
!! CAATAATCATTCAATCAAAA+TGG + chr7_3:32021410-32021429 Msa1101310:exon 20.0%
!! GAATAAAATTGAAAAATTGG+GGG - chr7_3:32021530-32021549 None:intergenic 20.0%
!! GGAATAAAATTGAAAAATTG+GGG - chr7_3:32021531-32021550 None:intergenic 20.0%
!!! TTTTAGTAGGAATTTACATT+GGG - chr7_3:32021638-32021657 None:intergenic 20.0%
!!! TTTTTAGTAGGAATTTACAT+TGG - chr7_3:32021639-32021658 None:intergenic 20.0%
!! TAATTTAACATCAAAAAAGG+GGG - chr7_3:32021700-32021719 None:intergenic 20.0%
!! GTAATTTAACATCAAAAAAG+GGG - chr7_3:32021701-32021720 None:intergenic 20.0%
!! GAGTAATTTAACATCAAAAA+AGG - chr7_3:32021703-32021722 None:intergenic 20.0%
!!! ATCATTATTTTTTGTTGTTG+TGG + chr7_3:32021760-32021779 Msa1101310:intron 20.0%
!! TAATAACAATTACATGAATG+AGG + chr7_3:32021884-32021903 Msa1101310:CDS 20.0%
!!! TTTTTTAAACTTTTATGGCT+TGG + chr7_3:32022025-32022044 Msa1101310:intron 20.0%
!!! ATCAAAAATGAATGTTGTTT+TGG + chr7_3:32022903-32022922 Msa1101310:intron 20.0%
!!! TGTCAATGAAATTGTTTTTT+TGG + chr7_3:32022976-32022995 Msa1101310:intron 20.0%
!!! TTTTAGTGAATGCATAATTT+TGG + chr7_3:32023745-32023764 Msa1101310:three_prime_UTR 20.0%
!!! TTTAGTGAATGCATAATTTT+GGG + chr7_3:32023746-32023765 Msa1101310:three_prime_UTR 20.0%
!! TTGGGTTATGATTAATTAAT+GGG + chr7_3:32023770-32023789 Msa1101310:three_prime_UTR 20.0%
! CGTAGAGAATAGAAAATAAA+AGG - chr7_3:32021485-32021504 None:intergenic 25.0%
! CATCTATGGAATAGAAAAAT+TGG - chr7_3:32021552-32021571 None:intergenic 25.0%
! ACTTAATCACATTACCATTA+AGG - chr7_3:32022179-32022198 None:intergenic 25.0%
! ACATATAATGTAACTGTCTT+CGG - chr7_3:32022398-32022417 None:intergenic 25.0%
!!! AATTTTTTCCATATTGCTTC+TGG + chr7_3:32022514-32022533 Msa1101310:intron 25.0%
!!! TATTTTGGAAGTGCATAATT+TGG + chr7_3:32022819-32022838 Msa1101310:intron 25.0%
!!! TGAATGTTGTTTTGGAATTT+AGG + chr7_3:32022911-32022930 Msa1101310:intron 25.0%
!!! GAATGTTGTTTTGGAATTTA+GGG + chr7_3:32022912-32022931 Msa1101310:intron 25.0%
!!! AATGTTGTTTTGGAATTTAG+GGG + chr7_3:32022913-32022932 Msa1101310:intron 25.0%
! AACAATTTCATTGACAAAGA+AGG - chr7_3:32022972-32022991 None:intergenic 25.0%
! AACTCAGTCAAAATAACAAA+AGG - chr7_3:32023528-32023547 None:intergenic 25.0%
! AAGATTTATCATTGACATTG+TGG - chr7_3:32023668-32023687 None:intergenic 25.0%
!! TTAGTGAATGCATAATTTTG+GGG + chr7_3:32023747-32023766 Msa1101310:three_prime_UTR 25.0%
! GTTGGGTTATGATTAATTAA+TGG + chr7_3:32023769-32023788 Msa1101310:three_prime_UTR 25.0%
! TGGGTTATGATTAATTAATG+GGG + chr7_3:32023771-32023790 Msa1101310:three_prime_UTR 25.0%
AAATATTAGGGTTTGCGATT+GGG - chr7_3:32021381-32021400 None:intergenic 30.0%
! TTCTTTTCGCATTTTCTCAA+TGG - chr7_3:32021922-32021941 None:intergenic 30.0%
GAAAATGCGAAAAGAAATTG+AGG + chr7_3:32021926-32021945 Msa1101310:CDS 30.0%
AAAAGAAATTGAGGCAAATC+AGG + chr7_3:32021935-32021954 Msa1101310:CDS 30.0%
TGTGAATTATTGTTGCAACA+AGG + chr7_3:32022087-32022106 Msa1101310:intron 30.0%
GTGAATTATTGTTGCAACAA+GGG + chr7_3:32022088-32022107 Msa1101310:intron 30.0%
TGAATTATTGTTGCAACAAG+GGG + chr7_3:32022089-32022108 Msa1101310:intron 30.0%
ATAGTAATGAACGACCTTAA+TGG + chr7_3:32022162-32022181 Msa1101310:intron 30.0%
AGCTTAAACAAGAAGAATGA+TGG - chr7_3:32022352-32022371 None:intergenic 30.0%
!! TTTGCTTTAAGTTGTTTGAG+AGG + chr7_3:32022446-32022465 Msa1101310:intron 30.0%
CTTTAAATCTTCAGCAAAGT+AGG - chr7_3:32022643-32022662 None:intergenic 30.0%
! TTTGCTGAAGATTTAAAGCA+AGG + chr7_3:32022645-32022664 Msa1101310:CDS 30.0%
AAATCAGAATGCAAGTCAAA+TGG - chr7_3:32022679-32022698 None:intergenic 30.0%
CCAGATCATACTAATTGTAT+AGG - chr7_3:32022739-32022758 None:intergenic 30.0%
CCTATACAATTAGTATGATC+TGG + chr7_3:32022736-32022755 Msa1101310:intron 30.0%
!! GAAGAGTGTGTTCAATATTT+TGG + chr7_3:32022804-32022823 Msa1101310:intron 30.0%
CATTACATGTTTCTTTAGAC+AGG - chr7_3:32022855-32022874 None:intergenic 30.0%
TCATTGACAAAGAAGGAAAA+GGG - chr7_3:32022965-32022984 None:intergenic 30.0%
TTCATTGACAAAGAAGGAAA+AGG - chr7_3:32022966-32022985 None:intergenic 30.0%
!! TTCACATTTGATTTTGTCAG+AGG + chr7_3:32023043-32023062 Msa1101310:intron 30.0%
!! TCACATTTGATTTTGTCAGA+GGG + chr7_3:32023044-32023063 Msa1101310:intron 30.0%
AGTCAGTTTACTGAAATTTC+AGG + chr7_3:32023402-32023421 Msa1101310:intron 30.0%
!! CTGAAATTTCAGGTATCTTT+TGG + chr7_3:32023412-32023431 Msa1101310:intron 30.0%
GTCAAAATAACAAAAGGAGA+GGG - chr7_3:32023522-32023541 None:intergenic 30.0%
AGTCAAAATAACAAAAGGAG+AGG - chr7_3:32023523-32023542 None:intergenic 30.0%
ACTTATGAACTTAGTTCAAC+AGG - chr7_3:32023695-32023714 None:intergenic 30.0%
TTAACAAAAAACACTGGGAT+TGG + chr7_3:32023988-32024007 Msa1101310:three_prime_UTR 30.0%
GAAATATTAGGGTTTGCGAT+TGG - chr7_3:32021382-32021401 None:intergenic 35.0%
TGGGGGAAAAATTAGAGTTA+GGG - chr7_3:32021513-32021532 None:intergenic 35.0%
TTGGGGGAAAAATTAGAGTT+AGG - chr7_3:32021514-32021533 None:intergenic 35.0%
AAGCTATGATAAACCAGACA+AGG + chr7_3:32021799-32021818 Msa1101310:intron 35.0%
ATTGAGTTTATGTACCGAGA+AGG + chr7_3:32021965-32021984 Msa1101310:CDS 35.0%
AACTAACCAGTTCATATGCT+TGG - chr7_3:32022004-32022023 None:intergenic 35.0%
!! AACTTTTATGGCTTGGACAA+AGG + chr7_3:32022032-32022051 Msa1101310:intron 35.0%
!! ACTTTTATGGCTTGGACAAA+GGG + chr7_3:32022033-32022052 Msa1101310:intron 35.0%
GTTCAGAAATGTAAATCCCT+AGG - chr7_3:32022115-32022134 None:intergenic 35.0%
! TCACTGTACAATTTTTCTCG+AGG + chr7_3:32022322-32022341 Msa1101310:intron 35.0%
ATGCATAACCAGAAGCAATA+TGG - chr7_3:32022525-32022544 None:intergenic 35.0%
CATTGACAAAGAAGGAAAAG+GGG - chr7_3:32022964-32022983 None:intergenic 35.0%
!! CACATTTGATTTTGTCAGAG+GGG + chr7_3:32023045-32023064 Msa1101310:intron 35.0%
TAATCAAGTCTCTTCGATTG+AGG - chr7_3:32023155-32023174 None:intergenic 35.0%
AATCGAAGAGACTTGATTAC+AGG + chr7_3:32023156-32023175 Msa1101310:CDS 35.0%
ATCGAAGAGACTTGATTACA+GGG + chr7_3:32023157-32023176 Msa1101310:CDS 35.0%
GGATCATATAGTCCTTCTTA+TGG - chr7_3:32023229-32023248 None:intergenic 35.0%
!! TGGGTTGATCAATATCCATA+GGG - chr7_3:32023250-32023269 None:intergenic 35.0%
!! ATGGGTTGATCAATATCCAT+AGG - chr7_3:32023251-32023270 None:intergenic 35.0%
GTAGGTTCATTTGGATCAAT+GGG - chr7_3:32023269-32023288 None:intergenic 35.0%
TGTAGGTTCATTTGGATCAA+TGG - chr7_3:32023270-32023289 None:intergenic 35.0%
ACACAGTATGTAGGTTCATT+TGG - chr7_3:32023278-32023297 None:intergenic 35.0%
TGATGACAAACACAGTATGT+AGG - chr7_3:32023287-32023306 None:intergenic 35.0%
TACATACTGTGTTTGTCATC+AGG + chr7_3:32023286-32023305 Msa1101310:CDS 35.0%
! TATAGTTTCAAGTCTCCTTG+TGG + chr7_3:32023340-32023359 Msa1101310:intron 35.0%
ATATGACACAATTGTCCACA+AGG - chr7_3:32023358-32023377 None:intergenic 35.0%
!! TATTATTCTTGATAGTGCCG+AGG + chr7_3:32023554-32023573 Msa1101310:intron 35.0%
!!! TGAATGCATAATTTTGGGGT+TGG + chr7_3:32023751-32023770 Msa1101310:three_prime_UTR 35.0%
!!! GAATGCATAATTTTGGGGTT+GGG + chr7_3:32023752-32023771 Msa1101310:three_prime_UTR 35.0%
AAGGGAAAGAGTTACATAAG+AGG - chr7_3:32023815-32023834 None:intergenic 35.0%
!! CACGTGTATTTTCTAGTCAA+GGG - chr7_3:32023833-32023852 None:intergenic 35.0%
CTTGACTAGAAAATACACGT+GGG + chr7_3:32023832-32023851 Msa1101310:three_prime_UTR 35.0%
AACCAGCAAGAACACTTCTA+GGG + chr7_3:32021346-32021365 Msa1101310:five_prime_UTR 40.0%
AGTCTACGTCGATGACTATT+TGG + chr7_3:32021449-32021468 Msa1101310:CDS 40.0%
!! CAGCAGACAAAGTACTGTAT+GGG + chr7_3:32021822-32021841 Msa1101310:CDS 40.0%
!! AGCAGACAAAGTACTGTATG+GGG + chr7_3:32021823-32021842 Msa1101310:CDS 40.0%
!! TCATCTCATGGCTCTAAGAA+GGG + chr7_3:32021849-32021868 Msa1101310:CDS 40.0%
!! ATGTACCGAGAAGGTTTTGT+TGG + chr7_3:32021974-32021993 Msa1101310:CDS 40.0%
ATGATTGCAGCTCCTATCTT+GGG + chr7_3:32022482-32022501 Msa1101310:intron 40.0%
ATTCACAAGACTCCCAAGAT+AGG - chr7_3:32022497-32022516 None:intergenic 40.0%
!! CCTCATCAAGTCGTTTTACA+TGG - chr7_3:32022614-32022633 None:intergenic 40.0%
CCATGTAAAACGACTTGATG+AGG + chr7_3:32022611-32022630 Msa1101310:CDS 40.0%
AAGGAAAAGGGGAATTTCAG+GGG - chr7_3:32022953-32022972 None:intergenic 40.0%
GAAGGAAAAGGGGAATTTCA+GGG - chr7_3:32022954-32022973 None:intergenic 40.0%
AGAAGGAAAAGGGGAATTTC+AGG - chr7_3:32022955-32022974 None:intergenic 40.0%
TTTAGGGACAATAGGCAATG+GGG - chr7_3:32023102-32023121 None:intergenic 40.0%
GTTTAGGGACAATAGGCAAT+GGG - chr7_3:32023103-32023122 None:intergenic 40.0%
!!! TTTTCGGGTTTAGGGACAAT+AGG - chr7_3:32023110-32023129 None:intergenic 40.0%
GAAGGACTATATGATCCCTA+TGG + chr7_3:32023232-32023251 Msa1101310:CDS 40.0%
TGGTTCCACTATTCATGTGT+TGG + chr7_3:32023584-32023603 Msa1101310:CDS 40.0%
TTAGGCCAACACATGAATAG+TGG - chr7_3:32023592-32023611 None:intergenic 40.0%
TGAACCTTGTCTCTTGTGTT+AGG - chr7_3:32023610-32023629 None:intergenic 40.0%
AACACAAGAGACAAGGTTCA+AGG + chr7_3:32023610-32023629 Msa1101310:CDS 40.0%
ACACAAGAGACAAGGTTCAA+GGG + chr7_3:32023611-32023630 Msa1101310:CDS 40.0%
TGTGGTAGTAATCTGCAAGT+TGG - chr7_3:32023650-32023669 None:intergenic 40.0%
!! CCACGTGTATTTTCTAGTCA+AGG - chr7_3:32023834-32023853 None:intergenic 40.0%
CCTTGACTAGAAAATACACG+TGG + chr7_3:32023831-32023850 Msa1101310:three_prime_UTR 40.0%
!! AAAAAACACTGGGATTGGTC+TGG + chr7_3:32023993-32024012 Msa1101310:three_prime_UTR 40.0%
!! TTCTTGCTGGTTCAGATACG+AGG - chr7_3:32021338-32021357 None:intergenic 45.0%
GAACCAGCAAGAACACTTCT+AGG + chr7_3:32021345-32021364 Msa1101310:five_prime_UTR 45.0%
!! AACCCTAGAAGTGTTCTTGC+TGG - chr7_3:32021351-32021370 None:intergenic 45.0%
AAAATGGCAATTGCACGCAC+TGG + chr7_3:32021426-32021445 Msa1101310:exon 45.0%
! GGGCAATGTATTGGCATCTA+TGG - chr7_3:32021566-32021585 None:intergenic 45.0%
!! AAGAAGTCTCTGAAGCTCAG+CGG - chr7_3:32021587-32021606 None:intergenic 45.0%
TCGTTCATCGAGTTCACGAA+CGG - chr7_3:32021614-32021633 None:intergenic 45.0%
!! GCAGCAGACAAAGTACTGTA+TGG + chr7_3:32021821-32021840 Msa1101310:CDS 45.0%
TGTATGGGGTTCTCATCTCA+TGG + chr7_3:32021837-32021856 Msa1101310:CDS 45.0%
!! CTCATCTCATGGCTCTAAGA+AGG + chr7_3:32021848-32021867 Msa1101310:CDS 45.0%
GATGATTGCAGCTCCTATCT+TGG + chr7_3:32022481-32022500 Msa1101310:intron 45.0%
TTAGGGACAATAGGCAATGG+GGG - chr7_3:32023101-32023120 None:intergenic 45.0%
GGTTTAGGGACAATAGGCAA+TGG - chr7_3:32023104-32023123 None:intergenic 45.0%
! ATATACGTGCCTGCGCTTTT+CGG - chr7_3:32023126-32023145 None:intergenic 45.0%
TTGATTACAGGGAGAGAGAC+TGG + chr7_3:32023168-32023187 Msa1101310:CDS 45.0%
TGATTACAGGGAGAGAGACT+GGG + chr7_3:32023169-32023188 Msa1101310:CDS 45.0%
TAGTCCTTCTTATGGCTTCC+TGG - chr7_3:32023221-32023240 None:intergenic 45.0%
TGAACTTTGCTCTCTTGTGC+TGG + chr7_3:32023473-32023492 Msa1101310:intron 45.0%
!! TATTCTTGATAGTGCCGAGG+AGG + chr7_3:32023557-32023576 Msa1101310:intron 45.0%
TTGGCCTAACACAAGAGACA+AGG + chr7_3:32023603-32023622 Msa1101310:CDS 45.0%
AGACAAGGTTCAAGGGAAAG+TGG + chr7_3:32023618-32023637 Msa1101310:CDS 45.0%
TTGCTCGACTATTTGTGTGC+CGG + chr7_3:32023878-32023897 Msa1101310:three_prime_UTR 45.0%
GGATTTGAGTAGTATGCACG+AGG + chr7_3:32024022-32024041 Msa1101310:three_prime_UTR 45.0%
!! TAAAAAAAGTAAATTTAATA+GGG - chr7_3:32021677-32021696 None:intergenic 5.0%
!! AAAAAAAAAAAAAATAAGAA+AGG - chr7_3:32023019-32023038 None:intergenic 5.0%
! AAAACGCTGTCGTTTGAGCC+TGG - chr7_3:32021300-32021319 None:intergenic 50.0%
GTTCAGATACGAGGAAGCGA+AGG - chr7_3:32021329-32021348 None:intergenic 50.0%
AAGCTCAGCGGGCAATGTAT+TGG - chr7_3:32021575-32021594 None:intergenic 50.0%
!! AGAAGTCTCTGAAGCTCAGC+GGG - chr7_3:32021586-32021605 None:intergenic 50.0%
ACTTTGTCTGCTGCCTTGTC+TGG - chr7_3:32021815-32021834 None:intergenic 50.0%
ATTGTTGCAACAAGGGGCCT+AGG + chr7_3:32022095-32022114 Msa1101310:intron 50.0%
TTGTTGCAACAAGGGGCCTA+GGG + chr7_3:32022096-32022115 Msa1101310:intron 50.0%
! TATACGTGCCTGCGCTTTTC+GGG - chr7_3:32023125-32023144 None:intergenic 50.0%
! GAAAAGCGCAGGCACGTATA+TGG + chr7_3:32023125-32023144 Msa1101310:CDS 50.0%
TTTGAGCTAATGCCTCCACC+AGG + chr7_3:32023200-32023219 Msa1101310:CDS 50.0%
TTCTTATGGCTTCCTGGTGG+AGG - chr7_3:32023215-32023234 None:intergenic 50.0%
TCCACCAGGAAGCCATAAGA+AGG + chr7_3:32023214-32023233 Msa1101310:CDS 50.0%
TCCTTCTTATGGCTTCCTGG+TGG - chr7_3:32023218-32023237 None:intergenic 50.0%
AGTGGAACCATTCACCTCCT+CGG - chr7_3:32023574-32023593 None:intergenic 50.0%
ATGTCATCACTGTCACCGCA+TGG - chr7_3:32023910-32023929 None:intergenic 50.0%
CAGATACGAGGAAGCGAAGG+TGG - chr7_3:32021326-32021345 None:intergenic 55.0%
GCGCGCCAACAAAACCTTCT+CGG - chr7_3:32021982-32022001 None:intergenic 55.0%
GGCAATGGGGGAAGAATTGC+TGG - chr7_3:32023089-32023108 None:intergenic 55.0%
! TCCCTAAACCCGAAAAGCGC+AGG + chr7_3:32023114-32023133 Msa1101310:CDS 55.0%
!!! GCCTGCGCTTTTCGGGTTTA+GGG - chr7_3:32023118-32023137 None:intergenic 55.0%
!!! TGCCTGCGCTTTTCGGGTTT+AGG - chr7_3:32023119-32023138 None:intergenic 55.0%
! GATAGTGCCGAGGAGGTGAA+TGG + chr7_3:32023564-32023583 Msa1101310:intron 55.0%
TGTCACCGCATGGTGTGAAC+CGG - chr7_3:32023900-32023919 None:intergenic 55.0%
!! ACTGGGATTGGTCTGGTGTG+AGG + chr7_3:32024000-32024019 Msa1101310:three_prime_UTR 55.0%
!! CTGGGATTGGTCTGGTGTGA+GGG + chr7_3:32024001-32024020 Msa1101310:three_prime_UTR 55.0%
GGCGCGCCAAGCATATGAAC+TGG + chr7_3:32021995-32022014 Msa1101310:CDS 60.0%
!! GTGTGCCGGTTCACACCATG+CGG + chr7_3:32023892-32023911 Msa1101310:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 32021281 32024055 32021281 ID=Msa1101310;Name=Msa1101310
chr7_3 mRNA 32021281 32024055 32021281 ID=Msa1101310-mRNA-1;Parent=Msa1101310;Name=Msa1101310-mRNA-1;_AED=0.19;_eAED=0.19;_QI=148|1|1|1|1|1|7|376|263
chr7_3 exon 32021281 32021474 32021281 ID=Msa1101310-mRNA-1:exon:6882;Parent=Msa1101310-mRNA-1
chr7_3 exon 32021569 32021640 32021569 ID=Msa1101310-mRNA-1:exon:6883;Parent=Msa1101310-mRNA-1
chr7_3 exon 32021802 32022016 32021802 ID=Msa1101310-mRNA-1:exon:6884;Parent=Msa1101310-mRNA-1
chr7_3 exon 32022603 32022666 32022603 ID=Msa1101310-mRNA-1:exon:6885;Parent=Msa1101310-mRNA-1
chr7_3 exon 32023063 32023307 32023063 ID=Msa1101310-mRNA-1:exon:6886;Parent=Msa1101310-mRNA-1
chr7_3 exon 32023424 32023462 32023424 ID=Msa1101310-mRNA-1:exon:6887;Parent=Msa1101310-mRNA-1
chr7_3 exon 32023569 32024055 32023569 ID=Msa1101310-mRNA-1:exon:6888;Parent=Msa1101310-mRNA-1
chr7_3 five_prime_UTR 32021281 32021428 32021281 ID=Msa1101310-mRNA-1:five_prime_utr;Parent=Msa1101310-mRNA-1
chr7_3 CDS 32021429 32021474 32021429 ID=Msa1101310-mRNA-1:cds;Parent=Msa1101310-mRNA-1
chr7_3 CDS 32021569 32021640 32021569 ID=Msa1101310-mRNA-1:cds;Parent=Msa1101310-mRNA-1
chr7_3 CDS 32021802 32022016 32021802 ID=Msa1101310-mRNA-1:cds;Parent=Msa1101310-mRNA-1
chr7_3 CDS 32022603 32022666 32022603 ID=Msa1101310-mRNA-1:cds;Parent=Msa1101310-mRNA-1
chr7_3 CDS 32023063 32023307 32023063 ID=Msa1101310-mRNA-1:cds;Parent=Msa1101310-mRNA-1
chr7_3 CDS 32023424 32023462 32023424 ID=Msa1101310-mRNA-1:cds;Parent=Msa1101310-mRNA-1
chr7_3 CDS 32023569 32023679 32023569 ID=Msa1101310-mRNA-1:cds;Parent=Msa1101310-mRNA-1
chr7_3 three_prime_UTR 32023680 32024055 32023680 ID=Msa1101310-mRNA-1:three_prime_utr;Parent=Msa1101310-mRNA-1
Gene Sequence

>Msa1101310

ATGGCAATTGCACGCACTGGAGTCTACGTCGATGACTATTTGGAGTATGCCAATACATTGCCCGCTGAGCTTCAGAGACTTCTTAACACCGTTCGTGAACTCGATGAACGATCCCAATCTATGATAAACCAGACAAGGCAGCAGACAAAGTACTGTATGGGGTTCTCATCTCATGGCTCTAAGAAGGGTAATCATAATTATAATAACAATTACATGAATGAGGATGATGATGCTTCCATTGAGAAAATGCGAAAAGAAATTGAGGCAAATCAGGACAGTGCATTGAGTTTATGTACCGAGAAGGTTTTGTTGGCGCGCCAAGCATATGAACTGATAGACAGCCATGTAAAACGACTTGATGAGGATTTAACCTACTTTGCTGAAGATTTAAAGCAAGAGGGGAAAATATCACAAGATGAGCCAGCAATTCTTCCCCCATTGCCTATTGTCCCTAAACCCGAAAAGCGCAGGCACGTATATGGTACACCTCAATCGAAGAGACTTGATTACAGGGAGAGAGACTGGGATAGAGACTTTGAGCTAATGCCTCCACCAGGAAGCCATAAGAAGGACTATATGATCCCTATGGATATTGATCAACCCATTGATCCAAATGAACCTACATACTGTGTTTGTCATCAGGTATCTTTTGGAGATATGATTGCTTGTGACAACGAAAATTGCCGAGGAGGTGAATGGTTCCACTATTCATGTGTTGGCCTAACACAAGAGACAAGGTTCAAGGGAAAGTGGTATTGTCCAACTTGCAGATTACTACCACAATGTCAATGA
Protein sequence

>Msa1101310

MAIARTGVYVDDYLEYANTLPAELQRLLNTVRELDERSQSMINQTRQQTKYCMGFSSHGSKKGNHNYNNNYMNEDDDASIEKMRKEIEANQDSALSLCTEKVLLARQAYELIDSHVKRLDEDLTYFAEDLKQEGKISQDEPAILPPLPIVPKPEKRRHVYGTPQSKRLDYRERDWDRDFELMPPPGSHKKDYMIPMDIDQPIDPNEPTYCVCHQVSFGDMIACDNENCRGGEWFHYSCVGLTQETRFKGKWYCPTCRLLPQCQ*