Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1037070 | XP_003625443.1 | 99.074 | 108 | 0 | 1 | 1 | 108 | 1 | 107 | 1.02e-69 | 214 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1037070 | G7KYK0 | 99.074 | 108 | 0 | 1 | 1 | 108 | 1 | 107 | 4.88e-70 | 214 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0816630 | Msa1037070 | 0.813276 | 2.778713e-51 | -8.615850e-47 |
| Msa1037070 | Msa1124920 | 0.942266 | 9.749222e-102 | -8.615850e-47 |
| Msa1037070 | Msa1238680 | 0.800175 | 1.630162e-48 | -8.615850e-47 |
| Msa1037070 | Msa1400690 | 0.936639 | 1.258594e-97 | -8.615850e-47 |
| Msa0863940 | Msa1037070 | 0.813933 | 1.991316e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1037070 | MtrunA17_Chr7g0262501 | 99.074 | 108 | 0 | 1 | 1 | 108 | 1 | 107 | 9.39e-74 | 214 |
| Msa1037070 | MtrunA17_Chr1g0183081 | 92.784 | 97 | 7 | 0 | 3 | 99 | 7 | 103 | 8.28e-65 | 191 |
| Msa1037070 | MtrunA17_Chr1g0188281 | 56.842 | 95 | 41 | 0 | 1 | 95 | 38 | 132 | 6.34e-38 | 124 |
| Msa1037070 | MtrunA17_Chr6g0476661 | 55.789 | 95 | 42 | 0 | 1 | 95 | 4 | 98 | 1.22e-37 | 122 |
| Msa1037070 | MtrunA17_Chr6g0476721 | 47.674 | 86 | 45 | 0 | 7 | 92 | 40 | 125 | 7.11e-25 | 90.9 |
| Msa1037070 | MtrunA17_Chr5g0394191 | 44.706 | 85 | 47 | 0 | 7 | 91 | 9 | 93 | 6.13e-23 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1037070 | AT3G04300.1 | 67.368 | 95 | 31 | 0 | 5 | 99 | 1 | 95 | 6.44e-46 | 143 |
| Msa1037070 | AT4G28703.1 | 63.366 | 101 | 28 | 1 | 6 | 97 | 7 | 107 | 2.27e-41 | 132 |
| Msa1037070 | AT4G10300.1 | 61.538 | 91 | 35 | 0 | 5 | 95 | 44 | 134 | 9.30e-40 | 129 |
| Msa1037070 | AT4G10280.1 | 36.559 | 93 | 56 | 1 | 7 | 96 | 48 | 140 | 7.32e-18 | 73.9 |
| Msa1037070 | AT4G10290.1 | 34.343 | 99 | 62 | 1 | 7 | 102 | 20 | 118 | 2.64e-13 | 61.6 |
Find 22 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTCTGAAGCCATGAGGTTT+TGG | 0.241413 | 7_1:+73585951 | None:intergenic |
| TTACATGCAATGTGGGATTT+TGG | 0.280274 | 7_1:+73586030 | None:intergenic |
| AATTTCAATTGGTACTTTCC+TGG | 0.284978 | 7_1:+73585729 | None:intergenic |
| CAAGTACAACTGAGTCCTTT+TGG | 0.330475 | 7_1:+73585603 | None:intergenic |
| CAAAAGGACTCAGTTGTACT+TGG | 0.347089 | 7_1:-73585602 | Msa1037070:CDS |
| CTGAGAAGGGTTTCTTTCAA+TGG | 0.378765 | 7_1:+73585919 | None:intergenic |
| CTTCTGCATCAAATTTCAAT+TGG | 0.414470 | 7_1:+73585718 | None:intergenic |
| AAAAGATGGGGTTGTTCTCC+AGG | 0.417937 | 7_1:-73585747 | Msa1037070:intron |
| AAACATGTTATTTATTGAAA+GGG | 0.428832 | 7_1:-73585695 | Msa1037070:CDS |
| GAAACATGTTATTTATTGAA+AGG | 0.444821 | 7_1:-73585696 | Msa1037070:CDS |
| AAAGTGAAAGCATACACAAA+AGG | 0.467313 | 7_1:-73585672 | Msa1037070:CDS |
| TTCTTTCAATGGTGATTCGA+AGG | 0.500926 | 7_1:+73585930 | None:intergenic |
| CAGCTAAACGTGACTGAGAA+GGG | 0.513726 | 7_1:+73585906 | None:intergenic |
| GACCTTGTCACCATTCCAAA+AGG | 0.519526 | 7_1:-73585618 | Msa1037070:CDS |
| AAGTGAAAGCATACACAAAA+GGG | 0.539574 | 7_1:-73585671 | Msa1037070:CDS |
| GTTCTCTTCCCAAAACCTCA+TGG | 0.549774 | 7_1:-73585960 | Msa1037070:exon |
| ATTAATAGTAATTAACAAGA+TGG | 0.549911 | 7_1:+73585510 | None:intergenic |
| CTGAATTGAACATCAAGTGC+TGG | 0.553924 | 7_1:-73585886 | Msa1037070:CDS |
| TCAGCTAAACGTGACTGAGA+AGG | 0.572317 | 7_1:+73585905 | None:intergenic |
| AAAAGGACTCAGTTGTACTT+GGG | 0.580307 | 7_1:-73585601 | Msa1037070:CDS |
| TTATCTCTTCTTCATGTCAG+AGG | 0.585717 | 7_1:+73585397 | None:intergenic |
| TTCGAAGGTCTGAAGCCATG+AGG | 0.682433 | 7_1:+73585945 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAACATGTTATTTATTGAAA+GGG | - | chr7_1:73585732-73585751 | Msa1037070:CDS | 15.0% |
| !! | ATTAATAGTAATTAACAAGA+TGG | + | chr7_1:73585920-73585939 | None:intergenic | 15.0% |
| !! | TTAAGAAAGAATACATACTT+GGG | + | chr7_1:73585567-73585586 | None:intergenic | 20.0% |
| !! | ATTAAGAAAGAATACATACT+TGG | + | chr7_1:73585568-73585587 | None:intergenic | 20.0% |
| !!! | TTTTTGCATTAATCTAACAA+AGG | - | chr7_1:73585618-73585637 | Msa1037070:CDS | 20.0% |
| !!! | GAAACATGTTATTTATTGAA+AGG | - | chr7_1:73585731-73585750 | Msa1037070:CDS | 20.0% |
| !!! | TTTTTTGTTACATGCAATGT+GGG | + | chr7_1:73585407-73585426 | None:intergenic | 25.0% |
| !!! | TTTTTTTGTTACATGCAATG+TGG | + | chr7_1:73585408-73585427 | None:intergenic | 25.0% |
| GGGGTGTAAAAATAAAAGAT+GGG | - | chr7_1:73585667-73585686 | Msa1037070:CDS | 30.0% | |
| GGGTGTAAAAATAAAAGATG+GGG | - | chr7_1:73585668-73585687 | Msa1037070:CDS | 30.0% | |
| AATTTCAATTGGTACTTTCC+TGG | + | chr7_1:73585701-73585720 | None:intergenic | 30.0% | |
| CTTCTGCATCAAATTTCAAT+TGG | + | chr7_1:73585712-73585731 | None:intergenic | 30.0% | |
| ! | AAAGTGAAAGCATACACAAA+AGG | - | chr7_1:73585755-73585774 | Msa1037070:intron | 30.0% |
| ! | AAGTGAAAGCATACACAAAA+GGG | - | chr7_1:73585756-73585775 | Msa1037070:intron | 30.0% |
| ! | TCATCAGATTTTGTAGAGTT+TGG | - | chr7_1:73585779-73585798 | Msa1037070:intron | 30.0% |
| TTCTTTCAATGGTGATTCGA+AGG | + | chr7_1:73585500-73585519 | None:intergenic | 35.0% | |
| !!! | GGTCCTTTTGTTTTGTAATG+TGG | - | chr7_1:73585639-73585658 | Msa1037070:CDS | 35.0% |
| CAGCCACATTACAAAACAAA+AGG | + | chr7_1:73585645-73585664 | None:intergenic | 35.0% | |
| !!! | TTTGTTTTGTAATGTGGCTG+TGG | - | chr7_1:73585645-73585664 | Msa1037070:CDS | 35.0% |
| !! | TTGTTTTGTAATGTGGCTGT+GGG | - | chr7_1:73585646-73585665 | Msa1037070:CDS | 35.0% |
| ! | GGGGGTGTAAAAATAAAAGA+TGG | - | chr7_1:73585666-73585685 | Msa1037070:CDS | 35.0% |
| AAAAGGACTCAGTTGTACTT+GGG | - | chr7_1:73585826-73585845 | Msa1037070:intron | 35.0% | |
| CTGAGAAGGGTTTCTTTCAA+TGG | + | chr7_1:73585511-73585530 | None:intergenic | 40.0% | |
| CTGAATTGAACATCAAGTGC+TGG | - | chr7_1:73585541-73585560 | Msa1037070:CDS | 40.0% | |
| ! | TGTTTTGTAATGTGGCTGTG+GGG | - | chr7_1:73585647-73585666 | Msa1037070:CDS | 40.0% |
| !! | GATTTTGTAGAGTTTGGAGC+TGG | - | chr7_1:73585785-73585804 | Msa1037070:intron | 40.0% |
| !! | ACAACTGAGTCCTTTTGGAA+TGG | + | chr7_1:73585822-73585841 | None:intergenic | 40.0% |
| ! | CAAGTACAACTGAGTCCTTT+TGG | + | chr7_1:73585827-73585846 | None:intergenic | 40.0% |
| CAAAAGGACTCAGTTGTACT+TGG | - | chr7_1:73585825-73585844 | Msa1037070:intron | 40.0% | |
| GTTCTCTTCCCAAAACCTCA+TGG | - | chr7_1:73585467-73585486 | Msa1037070:three_prime_UTR | 45.0% | |
| !! | GTCTGAAGCCATGAGGTTTT+GGG | + | chr7_1:73585478-73585497 | None:intergenic | 45.0% |
| CAGCTAAACGTGACTGAGAA+GGG | + | chr7_1:73585524-73585543 | None:intergenic | 45.0% | |
| TCAGCTAAACGTGACTGAGA+AGG | + | chr7_1:73585525-73585544 | None:intergenic | 45.0% | |
| ! | GTTTTGTAATGTGGCTGTGG+GGG | - | chr7_1:73585648-73585667 | Msa1037070:CDS | 45.0% |
| AAAAGATGGGGTTGTTCTCC+AGG | - | chr7_1:73585680-73585699 | Msa1037070:CDS | 45.0% | |
| GACCTTGTCACCATTCCAAA+AGG | - | chr7_1:73585809-73585828 | Msa1037070:intron | 45.0% | |
| !!! | GTCCTTTTGGAATGGTGACA+AGG | + | chr7_1:73585814-73585833 | None:intergenic | 45.0% |
| !! | GGTCTGAAGCCATGAGGTTT+TGG | + | chr7_1:73585479-73585498 | None:intergenic | 50.0% |
| ! | TTCGAAGGTCTGAAGCCATG+AGG | + | chr7_1:73585485-73585504 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 73585398 | 73586051 | 73585398 | ID=Msa1037070;Name=Msa1037070 |
| chr7_1 | mRNA | 73585398 | 73586051 | 73585398 | ID=Msa1037070-mRNA-1;Parent=Msa1037070;Name=Msa1037070-mRNA-1;_AED=0.01;_eAED=0.01;_QI=88|1|1|1|1|1|2|123|108 |
| chr7_1 | exon | 73585398 | 73585764 | 73585398 | ID=Msa1037070-mRNA-1:exon:18139;Parent=Msa1037070-mRNA-1 |
| chr7_1 | exon | 73585881 | 73586051 | 73585881 | ID=Msa1037070-mRNA-1:exon:18138;Parent=Msa1037070-mRNA-1 |
| chr7_1 | five_prime_UTR | 73585964 | 73586051 | 73585964 | ID=Msa1037070-mRNA-1:five_prime_utr;Parent=Msa1037070-mRNA-1 |
| chr7_1 | CDS | 73585881 | 73585963 | 73585881 | ID=Msa1037070-mRNA-1:cds;Parent=Msa1037070-mRNA-1 |
| chr7_1 | CDS | 73585521 | 73585764 | 73585521 | ID=Msa1037070-mRNA-1:cds;Parent=Msa1037070-mRNA-1 |
| chr7_1 | three_prime_UTR | 73585398 | 73585520 | 73585398 | ID=Msa1037070-mRNA-1:three_prime_utr;Parent=Msa1037070-mRNA-1 |
| Gene Sequence |
| Protein sequence |
