Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400690 | XP_003625443.1 | 99.074 | 108 | 0 | 1 | 1 | 108 | 1 | 107 | 1.02e-69 | 214 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400690 | G7KYK0 | 99.074 | 108 | 0 | 1 | 1 | 108 | 1 | 107 | 4.88e-70 | 214 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1037070 | Msa1400690 | 0.936639 | 1.258594e-97 | -8.615850e-47 |
| Msa1124920 | Msa1400690 | 0.982438 | 4.313849e-155 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400690 | MtrunA17_Chr7g0262501 | 99.074 | 108 | 0 | 1 | 1 | 108 | 1 | 107 | 9.39e-74 | 214 |
| Msa1400690 | MtrunA17_Chr1g0183081 | 92.784 | 97 | 7 | 0 | 3 | 99 | 7 | 103 | 8.28e-65 | 191 |
| Msa1400690 | MtrunA17_Chr1g0188281 | 56.842 | 95 | 41 | 0 | 1 | 95 | 38 | 132 | 6.34e-38 | 124 |
| Msa1400690 | MtrunA17_Chr6g0476661 | 55.789 | 95 | 42 | 0 | 1 | 95 | 4 | 98 | 1.22e-37 | 122 |
| Msa1400690 | MtrunA17_Chr6g0476721 | 47.674 | 86 | 45 | 0 | 7 | 92 | 40 | 125 | 7.11e-25 | 90.9 |
| Msa1400690 | MtrunA17_Chr5g0394191 | 44.706 | 85 | 47 | 0 | 7 | 91 | 9 | 93 | 6.13e-23 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400690 | AT3G04300.1 | 67.368 | 95 | 31 | 0 | 5 | 99 | 1 | 95 | 6.44e-46 | 143 |
| Msa1400690 | AT4G28703.1 | 63.366 | 101 | 28 | 1 | 6 | 97 | 7 | 107 | 2.27e-41 | 132 |
| Msa1400690 | AT4G10300.1 | 61.538 | 91 | 35 | 0 | 5 | 95 | 44 | 134 | 9.30e-40 | 129 |
| Msa1400690 | AT4G10280.1 | 36.559 | 93 | 56 | 1 | 7 | 96 | 48 | 140 | 7.32e-18 | 73.9 |
| Msa1400690 | AT4G10290.1 | 34.343 | 99 | 62 | 1 | 7 | 102 | 20 | 118 | 2.64e-13 | 61.6 |
Find 22 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTCTGAAGCCATGAGGTTT+TGG | 0.241413 | tig0022476:-8021 | None:intergenic |
| TTACATGCAATGTGGGATTT+TGG | 0.280274 | tig0022476:-7942 | None:intergenic |
| AATTTCAATTGGTACTTTCC+TGG | 0.284978 | tig0022476:-8243 | None:intergenic |
| CAAGTACAACTGAGTCCTTT+TGG | 0.330475 | tig0022476:-8369 | None:intergenic |
| CAAAAGGACTCAGTTGTACT+TGG | 0.347089 | tig0022476:+8370 | Msa1400690:CDS |
| CTGAGAAGGGTTTCTTTCAA+TGG | 0.378765 | tig0022476:-8053 | None:intergenic |
| CTTCTGCATCAAATTTCAAT+TGG | 0.414470 | tig0022476:-8254 | None:intergenic |
| AAAAGATGGGGTTGTTCTCC+AGG | 0.417937 | tig0022476:+8225 | Msa1400690:intron |
| AAACATGTTATTTATTGAAA+GGG | 0.428832 | tig0022476:+8277 | Msa1400690:CDS |
| GAAACATGTTATTTATTGAA+AGG | 0.444821 | tig0022476:+8276 | Msa1400690:CDS |
| AAAGTGAAAGCATACACAAA+AGG | 0.467313 | tig0022476:+8300 | Msa1400690:CDS |
| TTCTTTCAATGGTGATTCGA+AGG | 0.500926 | tig0022476:-8042 | None:intergenic |
| CAGCTAAACGTGACTGAGAA+GGG | 0.513726 | tig0022476:-8066 | None:intergenic |
| GACCTTGTCACCATTCCAAA+AGG | 0.519526 | tig0022476:+8354 | Msa1400690:CDS |
| AAGTGAAAGCATACACAAAA+GGG | 0.539574 | tig0022476:+8301 | Msa1400690:CDS |
| GTTCTCTTCCCAAAACCTCA+TGG | 0.549774 | tig0022476:+8012 | Msa1400690:exon |
| ATTAATAGTAATTAACAAGA+TGG | 0.549911 | tig0022476:-8462 | None:intergenic |
| CTGAATTGAACATCAAGTGC+TGG | 0.553924 | tig0022476:+8086 | Msa1400690:CDS |
| TCAGCTAAACGTGACTGAGA+AGG | 0.572317 | tig0022476:-8067 | None:intergenic |
| AAAAGGACTCAGTTGTACTT+GGG | 0.580307 | tig0022476:+8371 | Msa1400690:CDS |
| TTATCTCTTCTTCATGTCAG+AGG | 0.585717 | tig0022476:-8575 | None:intergenic |
| TTCGAAGGTCTGAAGCCATG+AGG | 0.682433 | tig0022476:-8027 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAACATGTTATTTATTGAAA+GGG | + | tig0022476:8277-8296 | Msa1400690:CDS | 15.0% |
| !! | ATTAATAGTAATTAACAAGA+TGG | - | tig0022476:8465-8484 | None:intergenic | 15.0% |
| !! | TTAAGAAAGAATACATACTT+GGG | - | tig0022476:8112-8131 | None:intergenic | 20.0% |
| !! | ATTAAGAAAGAATACATACT+TGG | - | tig0022476:8113-8132 | None:intergenic | 20.0% |
| !!! | TTTTTGCATTAATCTAACAA+AGG | + | tig0022476:8163-8182 | Msa1400690:intron | 20.0% |
| !!! | GAAACATGTTATTTATTGAA+AGG | + | tig0022476:8276-8295 | Msa1400690:CDS | 20.0% |
| !!! | TTTTTTGTTACATGCAATGT+GGG | - | tig0022476:7952-7971 | None:intergenic | 25.0% |
| !!! | TTTTTTTGTTACATGCAATG+TGG | - | tig0022476:7953-7972 | None:intergenic | 25.0% |
| GGGGTGTAAAAATAAAAGAT+GGG | + | tig0022476:8212-8231 | Msa1400690:intron | 30.0% | |
| GGGTGTAAAAATAAAAGATG+GGG | + | tig0022476:8213-8232 | Msa1400690:intron | 30.0% | |
| AATTTCAATTGGTACTTTCC+TGG | - | tig0022476:8246-8265 | None:intergenic | 30.0% | |
| CTTCTGCATCAAATTTCAAT+TGG | - | tig0022476:8257-8276 | None:intergenic | 30.0% | |
| ! | AAAGTGAAAGCATACACAAA+AGG | + | tig0022476:8300-8319 | Msa1400690:CDS | 30.0% |
| ! | AAGTGAAAGCATACACAAAA+GGG | + | tig0022476:8301-8320 | Msa1400690:CDS | 30.0% |
| ! | TCATCAGATTTTGTAGAGTT+TGG | + | tig0022476:8324-8343 | Msa1400690:CDS | 30.0% |
| TTCTTTCAATGGTGATTCGA+AGG | - | tig0022476:8045-8064 | None:intergenic | 35.0% | |
| !!! | GGTCCTTTTGTTTTGTAATG+TGG | + | tig0022476:8184-8203 | Msa1400690:intron | 35.0% |
| CAGCCACATTACAAAACAAA+AGG | - | tig0022476:8190-8209 | None:intergenic | 35.0% | |
| !!! | TTTGTTTTGTAATGTGGCTG+TGG | + | tig0022476:8190-8209 | Msa1400690:intron | 35.0% |
| !! | TTGTTTTGTAATGTGGCTGT+GGG | + | tig0022476:8191-8210 | Msa1400690:intron | 35.0% |
| ! | GGGGGTGTAAAAATAAAAGA+TGG | + | tig0022476:8211-8230 | Msa1400690:intron | 35.0% |
| AAAAGGACTCAGTTGTACTT+GGG | + | tig0022476:8371-8390 | Msa1400690:CDS | 35.0% | |
| CTGAGAAGGGTTTCTTTCAA+TGG | - | tig0022476:8056-8075 | None:intergenic | 40.0% | |
| CTGAATTGAACATCAAGTGC+TGG | + | tig0022476:8086-8105 | Msa1400690:CDS | 40.0% | |
| ! | TGTTTTGTAATGTGGCTGTG+GGG | + | tig0022476:8192-8211 | Msa1400690:intron | 40.0% |
| !! | GATTTTGTAGAGTTTGGAGC+TGG | + | tig0022476:8330-8349 | Msa1400690:CDS | 40.0% |
| !! | ACAACTGAGTCCTTTTGGAA+TGG | - | tig0022476:8367-8386 | None:intergenic | 40.0% |
| ! | CAAGTACAACTGAGTCCTTT+TGG | - | tig0022476:8372-8391 | None:intergenic | 40.0% |
| CAAAAGGACTCAGTTGTACT+TGG | + | tig0022476:8370-8389 | Msa1400690:CDS | 40.0% | |
| GTTCTCTTCCCAAAACCTCA+TGG | + | tig0022476:8012-8031 | Msa1400690:exon | 45.0% | |
| !! | GTCTGAAGCCATGAGGTTTT+GGG | - | tig0022476:8023-8042 | None:intergenic | 45.0% |
| CAGCTAAACGTGACTGAGAA+GGG | - | tig0022476:8069-8088 | None:intergenic | 45.0% | |
| TCAGCTAAACGTGACTGAGA+AGG | - | tig0022476:8070-8089 | None:intergenic | 45.0% | |
| ! | GTTTTGTAATGTGGCTGTGG+GGG | + | tig0022476:8193-8212 | Msa1400690:intron | 45.0% |
| AAAAGATGGGGTTGTTCTCC+AGG | + | tig0022476:8225-8244 | Msa1400690:intron | 45.0% | |
| GACCTTGTCACCATTCCAAA+AGG | + | tig0022476:8354-8373 | Msa1400690:CDS | 45.0% | |
| !!! | GTCCTTTTGGAATGGTGACA+AGG | - | tig0022476:8359-8378 | None:intergenic | 45.0% |
| !! | GGTCTGAAGCCATGAGGTTT+TGG | - | tig0022476:8024-8043 | None:intergenic | 50.0% |
| ! | TTCGAAGGTCTGAAGCCATG+AGG | - | tig0022476:8030-8049 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0022476 | gene | 7943 | 8596 | 7943 | ID=Msa1400690;Name=Msa1400690 |
| tig0022476 | mRNA | 7943 | 8596 | 7943 | ID=Msa1400690-mRNA-1;Parent=Msa1400690;Name=Msa1400690-mRNA-1;_AED=0.01;_eAED=0.01;_QI=88|1|1|1|1|1|2|123|108 |
| tig0022476 | exon | 7943 | 8113 | 7943 | ID=Msa1400690-mRNA-1:exon:17806;Parent=Msa1400690-mRNA-1 |
| tig0022476 | exon | 8230 | 8596 | 8230 | ID=Msa1400690-mRNA-1:exon:17807;Parent=Msa1400690-mRNA-1 |
| tig0022476 | five_prime_UTR | 7943 | 8030 | 7943 | ID=Msa1400690-mRNA-1:five_prime_utr;Parent=Msa1400690-mRNA-1 |
| tig0022476 | CDS | 8031 | 8113 | 8031 | ID=Msa1400690-mRNA-1:cds;Parent=Msa1400690-mRNA-1 |
| tig0022476 | CDS | 8230 | 8473 | 8230 | ID=Msa1400690-mRNA-1:cds;Parent=Msa1400690-mRNA-1 |
| tig0022476 | three_prime_UTR | 8474 | 8596 | 8474 | ID=Msa1400690-mRNA-1:three_prime_utr;Parent=Msa1400690-mRNA-1 |
| Gene Sequence |
| Protein sequence |
