AlfalfaGEDB | Msa1400690

M. sativa cultivar zhongmu-4 / Msa1400690

KEGG: K06995
GO: -
PFAM: PF05899.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1400690	XP_003625443.1	99.074	108	0	1	1	108	1	107	1.02e-69	214

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1400690	G7KYK0	99.074	108	0	1	1	108	1	107	4.88e-70	214

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1037070	Msa1400690	0.936639	1.258594e-97	-8.615850e-47
Msa1124920	Msa1400690	0.982438	4.313849e-155	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1400690	MtrunA17_Chr7g0262501	99.074	108	0	1	1	108	1	107	9.39e-74	214
Msa1400690	MtrunA17_Chr1g0183081	92.784	97	7	0	3	99	7	103	8.28e-65	191
Msa1400690	MtrunA17_Chr1g0188281	56.842	95	41	0	1	95	38	132	6.34e-38	124
Msa1400690	MtrunA17_Chr6g0476661	55.789	95	42	0	1	95	4	98	1.22e-37	122
Msa1400690	MtrunA17_Chr6g0476721	47.674	86	45	0	7	92	40	125	7.11e-25	90.9
Msa1400690	MtrunA17_Chr5g0394191	44.706	85	47	0	7	91	9	93	6.13e-23	85.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1400690	AT3G04300.1	67.368	95	31	0	5	99	1	95	6.44e-46	143
Msa1400690	AT4G28703.1	63.366	101	28	1	6	97	7	107	2.27e-41	132
Msa1400690	AT4G10300.1	61.538	91	35	0	5	95	44	134	9.30e-40	129
Msa1400690	AT4G10280.1	36.559	93	56	1	7	96	48	140	7.32e-18	73.9
Msa1400690	AT4G10290.1	34.343	99	62	1	7	102	20	118	2.64e-13	61.6

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Find 22 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATGGGGTTGTTCTCC+AGG	0.417937	tig0022476:+8225	Msa1400690:intron
AAAAGGACTCAGTTGTACTT+GGG	0.580307	tig0022476:+8371	Msa1400690:CDS
AAACATGTTATTTATTGAAA+GGG	0.428832	tig0022476:+8277	Msa1400690:CDS
AAAGTGAAAGCATACACAAA+AGG	0.467313	tig0022476:+8300	Msa1400690:CDS
AAGTGAAAGCATACACAAAA+GGG	0.539574	tig0022476:+8301	Msa1400690:CDS
AATTTCAATTGGTACTTTCC+TGG	0.284978	tig0022476:-8243	None:intergenic
ATTAATAGTAATTAACAAGA+TGG	0.549911	tig0022476:-8462	None:intergenic
CAAAAGGACTCAGTTGTACT+TGG	0.347089	tig0022476:+8370	Msa1400690:CDS
CAAGTACAACTGAGTCCTTT+TGG	0.330475	tig0022476:-8369	None:intergenic
CAGCTAAACGTGACTGAGAA+GGG	0.513726	tig0022476:-8066	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGGGTGTAAAAATAAAAGAT+GGG	+	tig0022476:8212-8231	Msa1400690:intron	30.0%
GGGTGTAAAAATAAAAGATG+GGG	+	tig0022476:8213-8232	Msa1400690:intron	30.0%
AATTTCAATTGGTACTTTCC+TGG	-	tig0022476:8246-8265	None:intergenic	30.0%
CTTCTGCATCAAATTTCAAT+TGG	-	tig0022476:8257-8276	None:intergenic	30.0%
TTCTTTCAATGGTGATTCGA+AGG	-	tig0022476:8045-8064	None:intergenic	35.0%
CAGCCACATTACAAAACAAA+AGG	-	tig0022476:8190-8209	None:intergenic	35.0%
AAAAGGACTCAGTTGTACTT+GGG	+	tig0022476:8371-8390	Msa1400690:CDS	35.0%
CTGAGAAGGGTTTCTTTCAA+TGG	-	tig0022476:8056-8075	None:intergenic	40.0%
CTGAATTGAACATCAAGTGC+TGG	+	tig0022476:8086-8105	Msa1400690:CDS	40.0%
CAAAAGGACTCAGTTGTACT+TGG	+	tig0022476:8370-8389	Msa1400690:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0022476	gene	7943	8596	7943	ID=Msa1400690;Name=Msa1400690
tig0022476	mRNA	7943	8596	7943	ID=Msa1400690-mRNA-1;Parent=Msa1400690;Name=Msa1400690-mRNA-1;_AED=0.01;_eAED=0.01;_QI=88\|1\|1\|1\|1\|1\|2\|123\|108
tig0022476	exon	7943	8113	7943	ID=Msa1400690-mRNA-1:exon:17806;Parent=Msa1400690-mRNA-1
tig0022476	exon	8230	8596	8230	ID=Msa1400690-mRNA-1:exon:17807;Parent=Msa1400690-mRNA-1
tig0022476	five_prime_UTR	7943	8030	7943	ID=Msa1400690-mRNA-1:five_prime_utr;Parent=Msa1400690-mRNA-1
tig0022476	CDS	8031	8113	8031	ID=Msa1400690-mRNA-1:cds;Parent=Msa1400690-mRNA-1
tig0022476	CDS	8230	8473	8230	ID=Msa1400690-mRNA-1:cds;Parent=Msa1400690-mRNA-1
tig0022476	three_prime_UTR	8474	8596	8474	ID=Msa1400690-mRNA-1:three_prime_utr;Parent=Msa1400690-mRNA-1

Gene Sequence	>Msa1400690 ATGGCTTCAGACCTTCGAATCACCATTGAAAGAAACCCTTCTCAGTCACGTTTAGCTGAATTGAACATCAAGTGCTGGCCCAAATGGGGTTGTTCTCCAGGAAAGTACCAATTGAAATTTGATGCAGAAGAAACATGTTATTTATTGAAAGGGAAAGTGAAAGCATACACAAAAGGGTCATCAGATTTTGTAGAGTTTGGAGCTGGAGACCTTGTCACCATTCCAAAAGGACTCAGTTGTACTTGGGATGTTTCTGTAGCTGTTGACAAGTACTACAAATTTGAATCATCTTCTTCTTCATCATCATCATCTTCACCATCTTGTTAA
Protein sequence	>Msa1400690 MASDLRITIERNPSQSRLAELNIKCWPKWGCSPGKYQLKFDAEETCYLLKGKVKAYTKGSSDFVEFGAGDLVTIPKGLSCTWDVSVAVDKYYKFESSSSSSSSSSPSC*